Rorug03G0128000

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017692
Physical Location & Seq
Forward (+)
10368391 .. 10370281
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0128000.1

Sequence Viewer

Length: 1425 bp
ATGGAGAAATCTCAATCTCACGTAGTGATCTTTCCATTTCCACTGCAAGGCCACATCAAACCCTTGCTATGCTTAGCAGAGCTTCTCAGCCATGCAGGCCTTCACGTCACCTTCGTCAACACCCGCCACAACCACAACCGCTTAGCGAACCTCCGTGCCCTCTCCGCCCACTTCCCCACCCTCCACTTCGAGTCCATCTTGGACGGTCTCCCCGATGACCACCCACGCACTTTGGGCAGCGAGTTGTTGATCGCATTGAAGACATCAATTAAGCCTCATTTTAAGGAGCTGCTCCAGTCATCCCTTAAGGCCAACGGTGTTGCGCTGCCGCCGCCAACATGCATTATAACAGATGGACTTGTGACTTTTGCCTTTGATGTTGCAGAAGAGTTGGGGTTGCCCATACTTAGTTTTGATGTTGGTTGTGCTCCTTACATGTGGACTTGTCTTTGTCTCCCCAACCTCATCGAACAAGGCCAGCTTCCTTTCCCAGATGATGACATGAATGCGGAAATCACCGGCGTCCCGGGAATGGAAGGCCTTCTGCGACGTGTAGATTTACCTGGTTTTTGCCGGGTAAAACAATCGAGCCACCCGGCTCTCCAATTCGCCATCCACGAAACTCAGACACAAAAACGAGCTTCAGCTCTAATCCTCGACACCATTTACGAACTTGATGCTCAGTGTCTATCCCATATGGCAACCATGTTCCCCAAAATTTACACTCTCGGCCCTCTCCACTCTCTCCTACATTCTCAAATCGGCGACGTCTCGCGATTATTAGCATCACACGGCGGTCTTGGGAAGGGGGATCCAAATTGCATGACATGGCTCGACTCTCAGCCAACCAAATCGGTTCTTTATGTCAGCTTCGGAACCCTAGTGACCTTGACACGTACCCAAATCATAGAGTTTTGGTACGGTCTGGTCAACAGTGGGCACCCGTTCTTGTGGGTTCTACAGTCCGACATCACTTCGGGTTTAGGTGAAGACCCGATTCCCATCGAGCTTGAAATTGGGACAAAAGAAAGAGGGTGCATAGTGAATTGGGTCTCGCAAGAGGAAGTCTTGGCTCACAAGTCATTGGGTGGGTTCTTGACCCACAACGGATGGAATTCTACAGTAGAGAGCATTGTAGCAGGAATTCCTATGATTTGTTGGCCTTATTTAGGGGATCAGCACATCACTAGCAGAAGTGTTTGTGAGAAGTGGAAGATTGGTCTTCGATTGAAAGAAAACTGTGACAGAAAGGACATTGAAACCATTATAAGAATGCTGCTGGAATTTAAGAGGGGAGAGATTCAGAGCTCAATGGACACAATTTCAAAAGTTGCTTGTGATAGTGTTGCGAAAGGTGGATCTTCAAACCACAACTTGGAGATGCTAATCCAAGACATTAGGAATATGCCTACAAGACCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

474

Amino Acids

52.54

Weight (kDa)

6.01

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_N PF26168 7 - 53 2.5e-06 Glycosyltransferase, N-terminal domain
UDPGT PF00201 276 - 427 8.9e-20 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18350
rosa_chinensis RchiOBHm_Chr3g0472831
rosa_laevigata RLG00000024052
rosa_multiflora Rmu_sc0005608.1_g000009
rosa_roxburghii Rroxscaffold_6G00408570
rosa_rugosa Rorug03G0128000
rosa_samantha Rh3AG180700 Rh3BG206900 Rh3CG204100 Rh3CG222500 Rh3DG169000
rosa_wichuraiana Rw3G016230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 347, 1268
AatII GACGTC 1 cut(s) 771
AccB1I GGYRCC 1 cut(s) 939
AccB7I CCANNNNNTGG 1 cut(s) 1375
AccII CGCG 1 cut(s) 775
AciI CCGC 7 cut(s) 124, 139, 165, 329, 332, 509, 795
AclWI GGATC 4 cut(s) 806, 819, 1182, 1366
AcsI RAATTY 4 cut(s) 717, 1114, 1143, 1283
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 2 cut(s) 522, 768
AdeI CACNNNGTG 1 cut(s) 25
AfaI GTAC 2 cut(s) 898, 920
AfiI CCNNNNNNNGG 4 cut(s) 47, 532, 1169, 1375
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 3 cut(s) 435, 550, 893
AgsI TTSAA 6 cut(s) 259, 1013, 1231, 1259, 1326, 1365
AjiI CACGTC 2 cut(s) 106, 551
AjnI CCWGG 1 cut(s) 562
AluBI AGCT 8 cut(s) 82, 289, 481, 641, 647, 870, 1009, 1308
AluI AGCT 8 cut(s) 82, 289, 481, 641, 647, 870, 1009, 1308
Alw21I GWGCWC 2 cut(s) 430, 1310
Alw26I GTCTC 4 cut(s) 212, 458, 775, 1057
AlwI GGATC 4 cut(s) 806, 819, 1182, 1366
Ama87I CYCGRG 1 cut(s) 526
AoxI GGCC 7 cut(s) 49, 97, 309, 475, 538, 730, 1160
ApeKI GCWGC 4 cut(s) 237, 289, 325, 1276
ApoI RAATTY 4 cut(s) 717, 1114, 1143, 1283
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 1 cut(s) 731
AsuC2I CCSGG 4 cut(s) 527, 528, 575, 596
AsuHPI GGTGA 3 cut(s) 100, 508, 998
AvaI CYCGRG 1 cut(s) 526
BaeGI GKGCMC 2 cut(s) 160, 942
BamHI GGATCC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 939
BanII GRGCYC 1 cut(s) 1310
BbsI GAAGAC 3 cut(s) 266, 996, 1214
Bbv12I GWGCWC 2 cut(s) 430, 1310
BbvI GCAGC 4 cut(s) 249, 276, 312, 1263
BccI CCATC 5 cut(s) 203, 347, 620, 1010, 1104
BceAI ACGGC 1 cut(s) 808
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 1 cut(s) 564
BcnI CCSGG 4 cut(s) 527, 528, 575, 596
BcoDI GTCTC 4 cut(s) 212, 458, 775, 1057
BfaI CTAG 2 cut(s) 881, 1188
BfmI CTRYAG 2 cut(s) 959, 1119
BfrI CTTAAG 1 cut(s) 305
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 6 cut(s) 238, 290, 326, 329, 332, 1277
BlpI GCTNAGC 2 cut(s) 73, 142
BlsI GCNGC 6 cut(s) 239, 291, 327, 330, 333, 1278
Bme1390I CCNGG 5 cut(s) 527, 528, 564, 575, 596
BmeT110I CYCGRG 1 cut(s) 526
BmgBI CACGTC 2 cut(s) 106, 551
BmgT120I GGNCC 1 cut(s) 731
BmiI GGNNCC 3 cut(s) 813, 877, 941
BmrFI CCNGG 5 cut(s) 527, 528, 564, 575, 596
BmsI GCATC 3 cut(s) 667, 794, 1371
BpiI GAAGAC 3 cut(s) 266, 996, 1214
BpmI CTGGAG 1 cut(s) 278
Bpu1102I GCTNAGC 2 cut(s) 73, 142
BpuMI CCSGG 4 cut(s) 527, 528, 575, 596
BsaAI YACGTR 2 cut(s) 22, 896
BsaBI GATNNNNATC 2 cut(s) 1001, 1385
BsaHI GRCGYC 2 cut(s) 522, 768
BsaI GGTCTC 2 cut(s) 212, 1057
BsaJI CCNNGG 1 cut(s) 526
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 532, 1169, 1375
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 295
Bse8I GATNNNNATC 2 cut(s) 1001, 1385
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 1 cut(s) 526
BseGI GGATG 3 cut(s) 299, 612, 1115
BseJI GATNNNNATC 2 cut(s) 1001, 1385
BseLI CCNNNNNNNGG 4 cut(s) 47, 532, 1169, 1375
BseMII CTCAG 4 cut(s) 100, 638, 695, 854
BseNI ACTGG 1 cut(s) 295
BseSI GKGCMC 2 cut(s) 160, 942
BseXI GCAGC 4 cut(s) 249, 276, 312, 1263
Bsh1236I CGCG 1 cut(s) 775
BshFI GGCC 7 cut(s) 51, 99, 311, 477, 540, 732, 1162
BshNI GGYRCC 1 cut(s) 939
BsiHKAI GWGCWC 2 cut(s) 430, 1310
BsiHKCI CYCGRG 1 cut(s) 526
BsiSI CCGG 4 cut(s) 519, 527, 574, 596
BslFI GGGAC 2 cut(s) 509, 1033
BslI CCNNNNNNNGG 4 cut(s) 47, 532, 1169, 1375
BsmAI GTCTC 4 cut(s) 212, 458, 775, 1057
BsmBI CGTCTC 1 cut(s) 775
BsmFI GGGAC 2 cut(s) 509, 1033
BsmI GAATGC 2 cut(s) 511, 1278
BsnI GGCC 7 cut(s) 51, 99, 311, 477, 540, 732, 1162
Bso31I GGTCTC 2 cut(s) 212, 1057
BsoBI CYCGRG 1 cut(s) 526
Bsp1286I GDGCHC 4 cut(s) 160, 430, 942, 1310
Bsp143I GATC 5 cut(s) 27, 249, 811, 1174, 1358
Bsp1720I GCTNAGC 2 cut(s) 73, 142
Bsp68I TCGCGA 1 cut(s) 775
BspACI CCGC 7 cut(s) 124, 139, 165, 329, 332, 509, 795
BspANI GGCC 7 cut(s) 51, 99, 311, 477, 540, 732, 1162
BspCNI CTCAG 4 cut(s) 99, 637, 694, 853
BspFNI CGCG 1 cut(s) 775
BspLI GGNNCC 3 cut(s) 813, 877, 941
BspPI GGATC 4 cut(s) 806, 819, 1182, 1366
BspT107I GGYRCC 1 cut(s) 939
BspTI CTTAAG 1 cut(s) 305
BspTNI GGTCTC 2 cut(s) 212, 1057
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 1 cut(s) 526
BssMI GATC 5 cut(s) 27, 249, 811, 1174, 1358
BssNI GRCGYC 2 cut(s) 522, 768
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 8 cut(s) 206, 317, 923, 935, 963, 1123, 1241, 1422
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 2 cut(s) 522, 768
BstAFI CTTAAG 1 cut(s) 305
BstBAI YACGTR 2 cut(s) 22, 896
BstC8I GCNNGC 2 cut(s) 97, 479
BstDEI CTNAG 7 cut(s) 73, 86, 142, 407, 624, 681, 840
BstENI CCTNNNNNAGG 1 cut(s) 1167
BstF5I GGATG 3 cut(s) 299, 612, 1115
BstFNI CGCG 1 cut(s) 775
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 5 cut(s) 30, 252, 814, 1177, 1361
BstMAI GTCTC 4 cut(s) 212, 458, 775, 1057
BstMBI GATC 5 cut(s) 27, 249, 811, 1174, 1358
BstMWI GCNNNNNNNGC 4 cut(s) 96, 164, 234, 331
BstNI CCWGG 1 cut(s) 564
BstNSI RCATGY 2 cut(s) 342, 439
BstSCI CCNGG 5 cut(s) 525, 526, 562, 573, 594
BstSFI CTRYAG 2 cut(s) 959, 1119
BstSLI GKGCMC 2 cut(s) 160, 942
BstUI CGCG 1 cut(s) 775
BstV1I GCAGC 4 cut(s) 249, 276, 312, 1263
BstV2I GAAGAC 3 cut(s) 266, 996, 1214
BstX2I RGATCY 2 cut(s) 811, 1358
BstYI RGATCY 2 cut(s) 811, 1358
BsuRI GGCC 7 cut(s) 51, 99, 311, 477, 540, 732, 1162
BtrI CACGTC 2 cut(s) 106, 551
BtsCI GGATG 3 cut(s) 299, 612, 1115
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 3 cut(s) 41, 689, 940
BtuMI TCGCGA 1 cut(s) 775
Cac8I GCNNGC 2 cut(s) 97, 479
CfoI GCGC 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 731
Cfr9I CCCGGG 1 cut(s) 526
CseI GACGC 1 cut(s) 511
CsiI ACCWGGT 1 cut(s) 562
Csp6I GTAC 2 cut(s) 897, 919
CspCI CAANNNNNGTGG 2 cut(s) 213, 248
CviAII CATG 7 cut(s) 92, 339, 436, 502, 706, 823, 828
CviQI GTAC 2 cut(s) 897, 919
DdeI CTNAG 7 cut(s) 73, 86, 142, 407, 624, 681, 840
DpnI GATC 5 cut(s) 29, 251, 813, 1176, 1360
DpnII GATC 5 cut(s) 27, 249, 811, 1174, 1358
DraIII CACNNNGTG 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EciI GGCGGA 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 1308
Eco147I AGGCCT 2 cut(s) 99, 540
Eco24I GRGCYC 1 cut(s) 1310
Eco31I GGTCTC 2 cut(s) 212, 1057
Eco53kI GAGCTC 1 cut(s) 1308
Eco57I CTGAAG 1 cut(s) 627
Eco88I CYCGRG 1 cut(s) 526
EcoICRI GAGCTC 1 cut(s) 1308
EcoNI CCTNNNNNAGG 1 cut(s) 1167
EcoRI GAATTC 2 cut(s) 1114, 1143
EcoRII CCWGG 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 1310
Esp3I CGTCTC 1 cut(s) 775
FaeI CATG 7 cut(s) 95, 342, 439, 505, 709, 826, 831
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 2 cut(s) 509, 1033
FatI CATG 7 cut(s) 91, 338, 435, 501, 705, 822, 827
FauI CCCGC 1 cut(s) 131
FauNDI CATATG 1 cut(s) 696
Fnu4HI GCNGC 6 cut(s) 238, 290, 326, 329, 332, 1277
FokI GGATG 3 cut(s) 286, 599, 1122
FriOI GRGCYC 1 cut(s) 1310
Fsp4HI GCNGC 6 cut(s) 238, 290, 326, 329, 332, 1277
FspBI CTAG 2 cut(s) 881, 1188
GlaI GCGC 1 cut(s) 324
GluI GCNGC 6 cut(s) 238, 290, 326, 329, 332, 1277
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 7 cut(s) 51, 99, 311, 477, 540, 732, 1162
HapII CCGG 4 cut(s) 519, 527, 574, 596
HgaI GACGC 1 cut(s) 511
HhaI GCGC 1 cut(s) 325
Hin1I GRCGYC 2 cut(s) 522, 768
Hin1II CATG 7 cut(s) 95, 342, 439, 505, 709, 826, 831
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 2 cut(s) 118, 931
HindII GTYRAC 2 cut(s) 118, 931
HinfI GANTC 4 cut(s) 191, 836, 997, 1300
HpaII CCGG 4 cut(s) 519, 527, 574, 596
HphI GGTGA 3 cut(s) 100, 508, 998
Hpy166II GTNNAC 3 cut(s) 118, 441, 931
Hpy188I TCNGA 4 cut(s) 627, 875, 967, 1305
Hpy188III TCNNGA 2 cut(s) 774, 1096
Hpy8I GTNNAC 3 cut(s) 118, 441, 931
Hpy99I CGWCG 2 cut(s) 552, 770
HpyAV CCTTC 5 cut(s) 110, 121, 530, 551, 799
HpyCH4III ACNGT 8 cut(s) 206, 317, 923, 935, 963, 1123, 1241, 1422
HpyCH4IV ACGT 5 cut(s) 21, 105, 550, 768, 895
HpyCH4V TGCA 6 cut(s) 46, 95, 342, 383, 822, 1038
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 164, 234, 331
HpyF3I CTNAG 7 cut(s) 73, 86, 142, 407, 624, 681, 840
HpySE526I ACGT 5 cut(s) 21, 105, 550, 768, 895
Hsp92I GRCGYC 2 cut(s) 522, 768
Hsp92II CATG 7 cut(s) 95, 342, 439, 505, 709, 826, 831
HspAI GCGC 1 cut(s) 323
Kzo9I GATC 5 cut(s) 27, 249, 811, 1174, 1358
LmnI GCTCC 3 cut(s) 286, 297, 433
Lsp1109I GCAGC 4 cut(s) 249, 276, 312, 1263
LweI GCATC 3 cut(s) 667, 794, 1371
MabI ACCWGGT 1 cut(s) 562
MaeI CTAG 2 cut(s) 881, 1188
MaeII ACGT 5 cut(s) 21, 105, 550, 768, 895
MaeIII GTNAC 4 cut(s) 106, 361, 883, 1241
MalI GATC 5 cut(s) 29, 251, 813, 1176, 1360
MboI GATC 5 cut(s) 27, 249, 811, 1174, 1358
MboII GAAGA 6 cut(s) 271, 398, 1001, 1214, 1225, 1353
MflI RGATCY 2 cut(s) 811, 1358
MhlI GDGCHC 4 cut(s) 160, 430, 942, 1310
MlyI GAGTC 2 cut(s) 200, 830
MmeI TCCRAC 1 cut(s) 990
Mph1103I ATGCAT 1 cut(s) 344
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 4 cut(s) 270, 282, 306, 1287
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 4 cut(s) 519, 527, 574, 596
MspR9I CCNGG 5 cut(s) 527, 528, 564, 575, 596
Mva1269I GAATGC 2 cut(s) 511, 1278
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 1 cut(s) 775
MwoI GCNNNNNNNGC 4 cut(s) 96, 164, 234, 331
NciI CCSGG 4 cut(s) 527, 528, 575, 596
NdeI CATATG 1 cut(s) 696
NdeII GATC 5 cut(s) 27, 249, 811, 1174, 1358
NlaIII CATG 7 cut(s) 95, 342, 439, 505, 709, 826, 831
NlaIV GGNNCC 3 cut(s) 813, 877, 941
NmeAIII GCCGAG 1 cut(s) 708
NmuCI GTSAC 4 cut(s) 106, 361, 883, 1241
NruI TCGCGA 1 cut(s) 775
NsiI ATGCAT 1 cut(s) 344
NspI RCATGY 2 cut(s) 342, 439
PceI AGGCCT 2 cut(s) 99, 540
PciI ACATGT 1 cut(s) 435
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 210, 615
PctI GAATGC 2 cut(s) 511, 1278
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 2 cut(s) 997, 1300
PflMI CCANNNNNTGG 1 cut(s) 1375
PkrI GCNGC 6 cut(s) 239, 291, 327, 330, 333, 1278
PleI GAGTC 2 cut(s) 199, 830
PpsI GAGTC 2 cut(s) 199, 830
Ppu21I YACGTR 2 cut(s) 22, 896
PscI ACATGT 1 cut(s) 435
PsiI TTATAA 2 cut(s) 347, 1268
Psp124BI GAGCTC 1 cut(s) 1310
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 3 cut(s) 813, 877, 941
PspPI GGNCC 1 cut(s) 731
PsuI RGATCY 2 cut(s) 811, 1358
RruI TCGCGA 1 cut(s) 775
RsaI GTAC 2 cut(s) 898, 920
RsaNI GTAC 2 cut(s) 897, 919
SacI GAGCTC 1 cut(s) 1310
SaqAI TTAA 4 cut(s) 270, 282, 306, 1287
SatI GCNGC 6 cut(s) 238, 290, 326, 329, 332, 1277
Sau3AI GATC 5 cut(s) 27, 249, 811, 1174, 1358
Sau96I GGNCC 1 cut(s) 731
SchI GAGTC 2 cut(s) 200, 830
ScrFI CCNGG 5 cut(s) 527, 528, 564, 575, 596
SduI GDGCHC 4 cut(s) 160, 430, 942, 1310
SexAI ACCWGGT 1 cut(s) 562
SfaNI GCATC 3 cut(s) 667, 794, 1371
SfcI CTRYAG 2 cut(s) 959, 1119
SgrAI CRCCGGYG 1 cut(s) 518
SmaI CCCGGG 1 cut(s) 528
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SseBI AGGCCT 2 cut(s) 99, 540
SsiI CCGC 7 cut(s) 124, 139, 165, 329, 332, 509, 795
SspMI CTAG 2 cut(s) 881, 1188
SstI GAGCTC 1 cut(s) 1310
StuI AGGCCT 2 cut(s) 99, 540
StyD4I CCNGG 5 cut(s) 525, 526, 562, 573, 594
TaaI ACNGT 8 cut(s) 206, 317, 923, 935, 963, 1123, 1241, 1422
TaiI ACGT 5 cut(s) 24, 108, 553, 771, 898
TaqI TCGA 7 cut(s) 189, 468, 587, 657, 834, 1005, 1225
TauI GCSGC 2 cut(s) 331, 334
TfiI GAWTC 2 cut(s) 997, 1300
Tru1I TTAA 4 cut(s) 270, 282, 306, 1287
Tru9I TTAA 4 cut(s) 270, 282, 306, 1287
TscAI CASTG 3 cut(s) 48, 689, 940
TseFI GTSAC 4 cut(s) 106, 361, 883, 1241
TseI GCWGC 4 cut(s) 237, 289, 325, 1276
Tsp45I GTSAC 4 cut(s) 106, 361, 883, 1241
TspDTI ATGAA 1 cut(s) 518
TspGWI ACGGA 2 cut(s) 143, 1122
TspMI CCCGGG 1 cut(s) 526
TspRI CASTG 3 cut(s) 48, 689, 940
Van91I CCANNNNNTGG 1 cut(s) 1375
Vha464I CTTAAG 1 cut(s) 305
XagI CCTNNNNNAGG 1 cut(s) 1167
XapI RAATTY 4 cut(s) 717, 1114, 1143, 1283
XceI RCATGY 2 cut(s) 342, 439
XmaI CCCGGG 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 2 cut(s) 881, 1188
ZraI GACGTC 1 cut(s) 769
Zsp2I ATGCAT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.