FvH4_6g18670

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014763
Physical Location & Seq
Reverse (-)
12325435 .. 12326175
741 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18670.t1

Sequence Viewer

Length: 741 bp
ATGGCCCCAAGAAATTATTCTATCTCCATGGCTCAATACTGTTGTTGCGTTCTCAAACCGATTCCTGGAGGGTTTTCGTGGCGAAAACTTCCCAGATGGTTAAAATGTCTGGTCTTGTGCAAGTGCATAATCGGCCACATCACGTTCTGGTTTGGAGCGTTCCTATTGATTTTCTGGCTGGTCTTCCTTCCTCACGAGCCTAGATTTACTGTTACGGATGCCCCTCTAACCCAATTCATTTTCAGCAGCAGTGACAGCAGCACCGGTACTAATCTTCACTACCACCTCGCCCTCAACATCACCATCAGAAACCCCAATAGAAGGGTCGGCATTTACTACCGCAGCATGACAGTGTCTGCTAGCTACAGAAAGAAGAAGTTTGGCCTGGTAAATTTGACGGATCCGGTACCATTTTACCAAGGCCACAAGAACACAACTCTCTTGCATCATCTTGTGCTTAAAGGGCAGCAGCAGTTGGTGAAATTTGGGAAGCGTGACATCTCCCAGTTTGACAAGGAGACTGTTGCTGGGGTTTATAGTATTAATGTGCAGGTAACTTTTCGGGTCAACGTCAGGTATGGAAAGAACAAGGTGAAGTTTTTGAAACCGGAAACCTACGATGACCAACATATCAACTGCAAGCTGAAACTTCCTTTCAGTGTTACTAATGAGACATCTGGTAATGGTTTCAAGGCTACAAAGTGCGATGGTGTTGGTATATTCAAGGACTTACCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

247

Amino Acids

28.23

Weight (kDa)

9.78

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 100 - 195 6.2e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 541
Acc65I GGTACC 1 cut(s) 406
AccB1I GGYRCC 1 cut(s) 406
AciI CCGC 1 cut(s) 340
AclWI GGATC 2 cut(s) 395, 408
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 2 cut(s) 391, 482
AfaI GTAC 2 cut(s) 268, 408
AfiI CCNNNNNNNGG 2 cut(s) 65, 321
AgeI ACCGGT 1 cut(s) 263
AgsI TTSAA 3 cut(s) 604, 691, 724
AjnI CCWGG 2 cut(s) 64, 384
AjuI GAANNNNNNNTTGG 1 cut(s) 33
AluBI AGCT 2 cut(s) 363, 643
AluI AGCT 2 cut(s) 363, 643
Alw26I GTCTC 2 cut(s) 512, 665
AlwI GGATC 2 cut(s) 395, 408
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 4 cut(s) 3, 133, 382, 421
ApeKI GCWGC 5 cut(s) 246, 258, 342, 466, 469
ApoI RAATTY 2 cut(s) 391, 482
AseI ATTAAT 1 cut(s) 543
AsiGI ACCGGT 1 cut(s) 263
Asp700I GAANNNNTTC 1 cut(s) 16
Asp718I GGTACC 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 3 cut(s) 292, 490, 604
AsuNHI GCTAGC 1 cut(s) 359
BamHI GGATCC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 406
BauI CACGAG 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 175
BbvI GCAGC 5 cut(s) 258, 270, 354, 478, 481
BccI CCATC 3 cut(s) 90, 311, 701
BciT130I CCWGG 2 cut(s) 66, 386
BcoDI GTCTC 2 cut(s) 512, 665
BfaI CTAG 2 cut(s) 201, 360
BfmI CTRYAG 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 541
BisI GCNGC 5 cut(s) 247, 259, 343, 467, 470
BlsI GCNGC 5 cut(s) 248, 260, 344, 468, 471
Bme1390I CCNGG 2 cut(s) 66, 386
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 3 cut(s) 6, 402, 408
BmrFI CCNGG 2 cut(s) 66, 386
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 2 cut(s) 208, 454
BmtI GCTAGC 1 cut(s) 363
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 1 cut(s) 175
BpmI CTGGAG 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 27, 418
BsaWI WCCGGW 3 cut(s) 263, 403, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 321
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 505
BseBI CCWGG 2 cut(s) 66, 386
BseDI CCNNGG 2 cut(s) 27, 418
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 2 cut(s) 65, 321
BseNI ACTGG 1 cut(s) 505
BseXI GCAGC 5 cut(s) 258, 270, 354, 478, 481
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 569
BshFI GGCC 4 cut(s) 5, 135, 384, 423
BshNI GGYRCC 1 cut(s) 406
BshTI ACCGGT 1 cut(s) 263
BsiSI CCGG 3 cut(s) 264, 404, 608
BslI CCNNNNNNNGG 2 cut(s) 65, 321
BsmAI GTCTC 2 cut(s) 512, 665
BsnI GGCC 4 cut(s) 5, 135, 384, 423
Bsp143I GATC 1 cut(s) 400
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 1 cut(s) 340
BspANI GGCC 4 cut(s) 5, 135, 384, 423
BspLI GGNNCC 3 cut(s) 6, 402, 408
BspMI ACCTGC 1 cut(s) 541
BspOI GCTAGC 1 cut(s) 363
BspPI GGATC 2 cut(s) 395, 408
BspT107I GGYRCC 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 1 cut(s) 505
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 27, 418
BssMI GATC 1 cut(s) 400
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 2 cut(s) 27, 418
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 2 cut(s) 66, 386
Bst4CI ACNGT 4 cut(s) 41, 211, 352, 523
BstC8I GCNNGC 2 cut(s) 361, 641
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 2 cut(s) 512, 665
BstMBI GATC 1 cut(s) 400
BstMWI GCNNNNNNNGC 3 cut(s) 132, 255, 463
BstNI CCWGG 2 cut(s) 66, 386
BstSCI CCNGG 2 cut(s) 64, 384
BstSFI CTRYAG 1 cut(s) 364
BstV1I GCAGC 5 cut(s) 258, 270, 354, 478, 481
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 4 cut(s) 5, 135, 384, 423
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 1 cut(s) 223
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 256, 357, 664
BveI ACCTGC 1 cut(s) 541
Cac8I GCNNGC 2 cut(s) 361, 641
CaiI CAGNNNCTG 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 267, 407
CspAI ACCGGT 1 cut(s) 263
CviAII CATG 2 cut(s) 28, 346
CviQI GTAC 2 cut(s) 267, 407
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 27, 418
EcoRII CCWGG 2 cut(s) 64, 384
EcoT14I CCWWGG 2 cut(s) 27, 418
ErhI CCWWGG 2 cut(s) 27, 418
FaeI CATG 2 cut(s) 31, 349
FaiI YATR 8 cut(s) 29, 128, 347, 537, 579, 630, 719, 736
FatI CATG 2 cut(s) 27, 345
Fnu4HI GCNGC 5 cut(s) 247, 259, 343, 467, 470
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 247, 259, 343, 467, 470
FspBI CTAG 2 cut(s) 201, 360
GluI GCNGC 5 cut(s) 247, 259, 343, 467, 470
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 4 cut(s) 5, 135, 384, 423
HapII CCGG 3 cut(s) 264, 404, 608
Hin1II CATG 2 cut(s) 31, 349
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 1 cut(s) 61
HpaII CCGG 3 cut(s) 264, 404, 608
HphI GGTGA 3 cut(s) 292, 490, 604
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 1 cut(s) 308
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 197, 315
HpyCH4III ACNGT 4 cut(s) 41, 211, 352, 523
HpyCH4IV ACGT 2 cut(s) 143, 570
HpyCH4V TGCA 5 cut(s) 120, 126, 445, 550, 639
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 255, 463
HpySE526I ACGT 2 cut(s) 143, 570
Hsp92II CATG 2 cut(s) 31, 349
KpnI GGTACC 1 cut(s) 410
Kzo9I GATC 1 cut(s) 400
LmnI GCTCC 1 cut(s) 155
Lsp1109I GCAGC 5 cut(s) 258, 270, 354, 478, 481
LweI GCATC 2 cut(s) 208, 454
MaeI CTAG 2 cut(s) 201, 360
MaeII ACGT 2 cut(s) 143, 570
MaeIII GTNAC 5 cut(s) 211, 251, 494, 553, 661
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MboII GAAGA 3 cut(s) 175, 266, 385
MflI RGATCY 1 cut(s) 400
MluCI AATT 4 cut(s) 13, 233, 391, 482
MnlI CCTC 5 cut(s) 62, 201, 234, 296, 302
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 3 cut(s) 101, 459, 543
MslI CAYNNNNRTG 1 cut(s) 350
MspI CCGG 3 cut(s) 264, 404, 608
MspR9I CCNGG 2 cut(s) 66, 386
MvaI CCWGG 2 cut(s) 66, 386
MwoI GCNNNNNNNGC 3 cut(s) 132, 255, 463
NcoI CCATGG 1 cut(s) 27
NdeII GATC 1 cut(s) 400
NheI GCTAGC 1 cut(s) 359
NlaIII CATG 2 cut(s) 31, 349
NlaIV GGNNCC 3 cut(s) 6, 402, 408
NmuCI GTSAC 2 cut(s) 251, 494
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 61
PflFI GACNNNGTC 1 cut(s) 352
PfoI TCCNGGA 1 cut(s) 64
PinAI ACCGGT 1 cut(s) 263
PkrI GCNGC 5 cut(s) 248, 260, 344, 468, 471
PshBI ATTAAT 1 cut(s) 543
Psp6I CCWGG 2 cut(s) 64, 384
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 2 cut(s) 64, 384
PspN4I GGNNCC 3 cut(s) 6, 402, 408
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 400
PsyI GACNNNGTC 1 cut(s) 352
RsaI GTAC 2 cut(s) 268, 408
RsaNI GTAC 2 cut(s) 267, 407
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 3 cut(s) 101, 459, 543
SatI GCNGC 5 cut(s) 247, 259, 343, 467, 470
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 66, 386
SetI ASST 9 cut(s) 146, 288, 365, 555, 573, 578, 594, 617, 645
SfaNI GCATC 2 cut(s) 208, 454
SfcI CTRYAG 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 350
Sse9I AATT 4 cut(s) 13, 233, 391, 482
SsiI CCGC 1 cut(s) 340
SspMI CTAG 2 cut(s) 201, 360
StyD4I CCNGG 2 cut(s) 64, 384
StyI CCWWGG 2 cut(s) 27, 418
TaaI ACNGT 4 cut(s) 41, 211, 352, 523
TaiI ACGT 2 cut(s) 146, 573
TasI AATT 4 cut(s) 13, 233, 391, 482
TfiI GAWTC 1 cut(s) 61
Tru1I TTAA 3 cut(s) 101, 459, 543
Tru9I TTAA 3 cut(s) 101, 459, 543
TscAI CASTG 3 cut(s) 256, 357, 664
TseFI GTSAC 2 cut(s) 251, 494
TseI GCWGC 5 cut(s) 246, 258, 342, 466, 469
Tsp45I GTSAC 2 cut(s) 251, 494
TspDTI ATGAA 1 cut(s) 226
TspGWI ACGGA 2 cut(s) 230, 413
TspRI CASTG 3 cut(s) 256, 357, 664
Tth111I GACNNNGTC 1 cut(s) 352
VspI ATTAAT 1 cut(s) 543
XapI RAATTY 2 cut(s) 391, 482
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 2 cut(s) 201, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.