FvH4_6g35120
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0018061
Physical Location & Seq
Reverse (-)
27762024 .. 27765230
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35120.t1

Sequence Viewer

Length: 2178 bp
ATGGCTGCAGCTGCCGGGCTCAATCTTCACCGCCCGTACCTCCTCCTCTGCATAATCTTCCTCTCCTTTTCCCCATTGACATATTCGATAACCGATGCCGAAGCGTTGCTCAAGTTCAAGCAATCACTCACCAATGCATCTGCTTTGGATTCTTGGGTGCCTAACTCTGCACCATGCACGTCAGGTACGCAATGGGCAGGAACCGTATGCTTTAATGGGAACGTTACGGGTCTTCGTCTAGGGGGAATGGGTTTGTCTGGCACACTAGACTTTAAATCGCTTATCGAAGTCAAGAGTCTTCGAAGCATGAGCGTAAAGAACAACAATTTCTCCGGCTCCATCCCGGATTTCAACGTCATTGGCCCCTTGAAGGCTATCTATTTGTCCGGGAATCAGTTTTCCGGTGAGATCCCATCCGACTTTTTCACCGACATGGACTCGTTGAGGAAAGTCTGGCTGGACCGCAATAATTTCAGTGGGAAAATTCCTGCATCTCTTGCGGAAACTCCGAATCTGCAGGTACTGCGTCTCGAGGACAATGAATTCAGTGGAAGCCTCCCGGATATGAGCCAAGTTACTTTGACGGTCCTCAATGTGTCTAATAACAAGCTGGAGGGGGAGATTCCGAAGAGTCTGCAGGATAAATTTGATAAGACTGTGTTTTCCGGAAACTCAGGTCTTTGTGGGTTGAATGTGGGCAAGGAATGTACTAATGCACCATCACCATCACCATCACCATCACCAACGAGTTCTAATGAGGGTAGTGGCTCAAAATCGAGTTCGAAAAGTAAGAAGGGAAGCTCTACAATTGTTATTGCATTGTCAGTAACTTGCGCGGTGGTGCTTCTCATTCTTATCCTTCTATTGTTTGTCAGATCAAGGAGAAAGAAGGCAGATGAGAATATGGATAGGCTTGGAACCCAAAACACTCAAAGCTATGATCATTCTCCTTCTCCTCAACAAGCACAAGTAGAAGCGGTTGAGTTGAATGTGGCGGAGATCAATCGTAGGGAAAGGGAAGTAGGGGAGTCATCTGCATCAGTTAGTAATGCTAGAAAGAGTACTAGCTCGGCGCGCAAGAGCACTTCGAGTCATGCTAAAGGATTAGGCGGGATGCCGGAGCTAGTGATGTTGAATGAAGAGAAGGGAGTGTTTGGGTTGACGGATTTGATGAAGGCCGCAGCCGAGGTACTTGGAAATGGGGGGATGGGTTCATCTTACAAGGCTGTCATGGCGAATGGGTTGGCGGTTGTGGTAAAAAGGATAAGAGAAATGAATGCAATGAGTAGAGAGGGGTTTGATGCAGAGATGAGGAGGCTTGGGACTCTACGCCATTGGAATGTCTTGACTCCTCTGGCTTATCACTACAGAAAAGAAGAGAAGCTGTTAGTTTATGAGTACATCCCCAAGAGCAGCTTGCTTTATCTATTACATGGTGATCGGGGATCGTCTCACGACCAACTTAACTGGCCTGCTAGGTTAAAGATTATTCAAGGAACAGCAAGAGGGATGGGTTTTATCCATAATGAATGTTCAGGCTATGATTTGCCCCATGGCGACCTAAAGTCAAGCAATGTACTAATTGGCCCCGACTATGAGCCCTTGCTTTCAGAGTTTGCATTTGCTCCAATGATCAACTCTTCACATTTACCACATGCCTTGTTTGCATACAAAGCCCCAGAAGCTTCAGAGTATGGCCTAGTATCACCCAAGTGCGATGTGTTCTGTCTAGGAGTCCTCATACTTGAAACCCTAACGGGCAAATTCCCTTCTCAGTATCTCAACCATGGCCAAGGTGGGACTGATGTAGTCCAATGGGTGCGGTCGGCTATGGCAGAAAGAAGAGAAATGGAACTATTAGACCCCGAGATTTCAAACGAACAGGGTTCAATCAGTGAAATGGAGAAGCTCCTCCACATTGGTGCAGCCTGCACAGAGAATGATCCGAGGCAACGGTTAGACATGATGGAGGCTATTCGAAGGATAGAGCAAATAAATGCCGTAGGAACTAGTGAAGAGGCTGCTAGAGACGGTTATGCAAACTCTTCTTTAGCAGTACCAAAGACAACTCATGTACCAACATTGCGAGAAGGGGTAGCAGATATGCCTGCACGGCCAGCTTCTGAGTCCGAGATCATCAGACTGGCATCGCGCAGCTTCCGAAGCAGATCGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

726

Amino Acids

78.77

Weight (kDa)

6.73

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 70 5.4e-07 Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr PF07714 395 - 658 1.4e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 396 - 659 4.1e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018061)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35120
prunus_persica Prupe.3G037900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0146351
rosa_laevigata RLG00000020196
rosa_multiflora Rmu_sc0002054.1_g000009
rosa_rugosa Rorug02G0394100
rosa_samantha Rh2AG450600 Rh2BG463000 Rh2CG437400 Rh2DG472000
rosa_wichuraiana Rw2G036780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 508
AccB1I GGYRCC 1 cut(s) 157
AccII CGCG 3 cut(s) 836, 1075, 2152
AccIII TCCGGA 1 cut(s) 665
AclI AACGTT 1 cut(s) 222
AclWI GGATC 3 cut(s) 403, 1453, 1937
AcoI YGGCCR 2 cut(s) 1789, 2114
AcsI RAATTY 4 cut(s) 483, 542, 644, 1763
AcuI CTGAAG 1 cut(s) 1671
AfiI CCNNNNNNNGG 1 cut(s) 370
AhdI GACNNNNNGTC 1 cut(s) 1564
AhlI ACTAGT 1 cut(s) 2009
AjiI CACGTC 1 cut(s) 180
AleI CACNNNNGTG 2 cut(s) 1711, 1920
Alw21I GWGCWC 1 cut(s) 1085
Alw26I GTCTC 3 cut(s) 533, 1455, 2022
AlwI GGATC 3 cut(s) 403, 1453, 1937
AlwNI CAGNNNCTG 2 cut(s) 523, 2123
Ama87I CYCGRG 2 cut(s) 530, 1865
Aor13HI TCCGGA 1 cut(s) 665
AoxI GGCC 7 cut(s) 361, 1176, 1469, 1585, 1696, 1789, 2114
ApeKI GCWGC 8 cut(s) 5, 8, 11, 1181, 1413, 1925, 2021, 2154
ApoI RAATTY 4 cut(s) 483, 542, 644, 1763
AspLEI GCGC 4 cut(s) 836, 1075, 1077, 2154
AspS9I GGNCC 4 cut(s) 362, 460, 586, 1586
AsuC2I CCSGG 4 cut(s) 16, 344, 388, 560
AsuII TTCGAA 3 cut(s) 301, 782, 1978
AvaI CYCGRG 2 cut(s) 530, 1865
AvaII GGWCC 2 cut(s) 460, 586
BaeI ACNNNNGTAYC 2 cut(s) 2058, 2091
BalI TGGCCA 1 cut(s) 1791
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 21, 1602
BbsI GAAGAC 2 cut(s) 224, 290
Bbv12I GWGCWC 1 cut(s) 1085
BbvI GCAGC 6 cut(s) 20, 1193, 1425, 1937, 2008, 2166
BccI CCATC 9 cut(s) 347, 421, 727, 733, 739, 745, 1201, 1504, 1960
BceAI ACGGC 2 cut(s) 1985, 2129
BcgI CGANNNNNNTGC 2 cut(s) 616, 650
BclI TGATCA 2 cut(s) 940, 1632
BcnI CCSGG 4 cut(s) 16, 344, 388, 560
BcoDI GTCTC 3 cut(s) 533, 1455, 2022
BcuI ACTAGT 1 cut(s) 2009
BfmI CTRYAG 4 cut(s) 6, 515, 635, 1366
BfuAI ACCTGC 1 cut(s) 508
BglI GCCNNNNNGGC 1 cut(s) 2113
BisI GCNGC 9 cut(s) 6, 9, 12, 1179, 1182, 1414, 1926, 2022, 2155
BlsI GCNGC 9 cut(s) 7, 10, 13, 1180, 1183, 1415, 1927, 2023, 2156
BmcAI AGTACT 1 cut(s) 1063
Bme1390I CCNGG 4 cut(s) 16, 344, 388, 560
Bme18I GGWCC 2 cut(s) 460, 586
BmeRI GACNNNNNGTC 1 cut(s) 1564
BmeT110I CYCGRG 2 cut(s) 530, 1865
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 4 cut(s) 362, 460, 586, 1586
BmiI GGNNCC 6 cut(s) 159, 202, 337, 364, 919, 1588
BmrFI CCNGG 4 cut(s) 16, 344, 388, 560
BmsI GCATC 7 cut(s) 85, 146, 500, 1046, 1104, 1291, 2156
BpiI GAAGAC 2 cut(s) 224, 290
BpmI CTGGAG 1 cut(s) 632
Bpu14I TTCGAA 3 cut(s) 301, 782, 1978
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 4 cut(s) 16, 344, 388, 560
BsaJI CCNNGG 5 cut(s) 1185, 1552, 1786, 1792, 1946
BsaWI WCCGGW 2 cut(s) 401, 665
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse1I ACTGG 2 cut(s) 1472, 2148
Bse3DI GCAATG 4 cut(s) 197, 1287, 1579, 2081
BseAI TCCGGA 1 cut(s) 665
BseDI CCNNGG 5 cut(s) 1185, 1552, 1786, 1792, 1946
BseGI GGATG 6 cut(s) 339, 413, 1119, 1212, 1401, 1515
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMI GCAATG 4 cut(s) 197, 1287, 1579, 2081
BseMII CTCAG 3 cut(s) 687, 1787, 2115
BseNI ACTGG 2 cut(s) 1472, 2148
BsePI GCGCGC 1 cut(s) 1073
BseRI GAGGAG 6 cut(s) 32, 35, 945, 1327, 1341, 1901
BseXI GCAGC 6 cut(s) 20, 1193, 1425, 1937, 2008, 2166
BsgI GTGCAG 4 cut(s) 153, 1915, 1944, 2094
Bsh1236I CGCG 3 cut(s) 836, 1075, 2152
Bsh1285I CGRYCG 1 cut(s) 1826
BshFI GGCC 7 cut(s) 363, 1178, 1471, 1587, 1698, 1791, 2116
BshNI GGYRCC 1 cut(s) 157
BsiEI CGRYCG 1 cut(s) 1826
BsiHKAI GWGCWC 1 cut(s) 1085
BsiHKCI CYCGRG 2 cut(s) 530, 1865
BsiSI CCGG 8 cut(s) 15, 333, 344, 387, 402, 560, 666, 1118
BslFI GGGAC 2 cut(s) 1336, 1813
BslI CCNNNNNNNGG 1 cut(s) 370
BsmAI GTCTC 3 cut(s) 533, 1455, 2022
BsmBI CGTCTC 3 cut(s) 533, 1455, 2022
BsmFI GGGAC 2 cut(s) 1336, 1813
BsmI GAATGC 1 cut(s) 1282
BsnI GGCC 7 cut(s) 363, 1178, 1471, 1587, 1698, 1791, 2116
BsoBI CYCGRG 2 cut(s) 530, 1865
Bsp119I TTCGAA 3 cut(s) 301, 782, 1978
Bsp1286I GDGCHC 3 cut(s) 21, 1085, 1602
Bsp13I TCCGGA 1 cut(s) 665
Bsp19I CCATGG 2 cut(s) 1552, 1786
BspANI GGCC 7 cut(s) 363, 1178, 1471, 1587, 1698, 1791, 2116
BspCNI CTCAG 3 cut(s) 686, 1786, 2116
BspEI TCCGGA 1 cut(s) 665
BspFNI CGCG 3 cut(s) 836, 1075, 2152
BspLI GGNNCC 6 cut(s) 159, 202, 337, 364, 919, 1588
BspMAI CTGCAG 3 cut(s) 10, 519, 639
BspMI ACCTGC 1 cut(s) 508
BspPI GGATC 3 cut(s) 403, 1453, 1937
BspT104I TTCGAA 3 cut(s) 301, 782, 1978
BspT107I GGYRCC 1 cut(s) 157
BsrDI GCAATG 4 cut(s) 197, 1287, 1579, 2081
BsrI ACTGG 2 cut(s) 1472, 2148
BssECI CCNNGG 5 cut(s) 1185, 1552, 1786, 1792, 1946
BssHII GCGCGC 1 cut(s) 1073
BssT1I CCWWGG 3 cut(s) 1552, 1786, 1792
Bst4CI ACNGT 5 cut(s) 205, 586, 658, 1956, 2033
Bst6I CTCTTC 7 cut(s) 623, 1134, 1371, 1645, 1837, 2010, 2050
BstAPI GCANNNNNTGC 2 cut(s) 497, 523
BstBI TTCGAA 3 cut(s) 301, 782, 1978
BstC8I GCNNGC 6 cut(s) 1075, 1418, 1473, 1930, 2109, 2118
BstDEI CTNAG 3 cut(s) 673, 1773, 2124
BstDSI CCRYGG 2 cut(s) 1552, 1786
BstF5I GGATG 6 cut(s) 339, 413, 1119, 1212, 1401, 1515
BstFNI CGCG 3 cut(s) 836, 1075, 2152
BstHHI GCGC 4 cut(s) 836, 1075, 1077, 2154
BstMAI GTCTC 3 cut(s) 533, 1455, 2022
BstMCI CGRYCG 1 cut(s) 1826
BstNSI RCATGY 1 cut(s) 1658
BstSCI CCNGG 4 cut(s) 14, 342, 386, 558
BstSFI CTRYAG 4 cut(s) 6, 515, 635, 1366
BstUI CGCG 3 cut(s) 836, 1075, 2152
BstV1I GCAGC 6 cut(s) 20, 1193, 1425, 1937, 2008, 2166
BstV2I GAAGAC 2 cut(s) 224, 290
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
BsuRI GGCC 7 cut(s) 363, 1178, 1471, 1587, 1698, 1791, 2116
BtgI CCRYGG 2 cut(s) 1552, 1786
BtgZI GCGATG 2 cut(s) 1731, 2133
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 6 cut(s) 339, 413, 1119, 1212, 1401, 1515
BtsIMutI CAGTG 3 cut(s) 481, 553, 1900
BveI ACCTGC 1 cut(s) 508
Cac8I GCNNGC 6 cut(s) 1075, 1418, 1473, 1930, 2109, 2118
CaiI CAGNNNCTG 2 cut(s) 523, 2123
CfoI GCGC 4 cut(s) 836, 1075, 1077, 2154
Cfr13I GGNCC 4 cut(s) 362, 460, 586, 1586
CseI GACGC 1 cut(s) 515
CspCI CAANNNNNGTGG 2 cut(s) 1641, 1676
DdeI CTNAG 3 cut(s) 673, 1773, 2124
DraI TTTAAA 1 cut(s) 274
DriI GACNNNNNGTC 1 cut(s) 1564
EaeI YGGCCR 2 cut(s) 1789, 2114
Eam1104I CTCTTC 7 cut(s) 623, 1134, 1371, 1645, 1837, 2010, 2050
Eam1105I GACNNNNNGTC 1 cut(s) 1564
EarI CTCTTC 7 cut(s) 623, 1134, 1371, 1645, 1837, 2010, 2050
EciI GGCGGA 1 cut(s) 1010
Eco130I CCWWGG 3 cut(s) 1552, 1786, 1792
Eco24I GRGCYC 2 cut(s) 21, 1602
Eco47I GGWCC 2 cut(s) 460, 586
Eco57I CTGAAG 1 cut(s) 1671
Eco88I CYCGRG 2 cut(s) 530, 1865
EcoRI GAATTC 1 cut(s) 542
EcoT14I CCWWGG 3 cut(s) 1552, 1786, 1792
EcoT22I ATGCAT 1 cut(s) 139
EcoT38I GRGCYC 2 cut(s) 21, 1602
ErhI CCWWGG 3 cut(s) 1552, 1786, 1792
Esp3I CGTCTC 3 cut(s) 533, 1455, 2022
FalI AAGNNNNNCTT 2 cut(s) 1400, 1432
FaqI GGGAC 2 cut(s) 1336, 1813
FauI CCCGC 1 cut(s) 1103
FbaI TGATCA 2 cut(s) 940, 1632
Fnu4HI GCNGC 9 cut(s) 6, 9, 12, 1179, 1182, 1414, 1926, 2022, 2155
FokI GGATG 6 cut(s) 326, 400, 1126, 1219, 1388, 1522
FriOI GRGCYC 2 cut(s) 21, 1602
Fsp4HI GCNGC 9 cut(s) 6, 9, 12, 1179, 1182, 1414, 1926, 2022, 2155
GlaI GCGC 4 cut(s) 835, 1074, 1076, 2153
GluI GCNGC 9 cut(s) 6, 9, 12, 1179, 1182, 1414, 1926, 2022, 2155
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 7 cut(s) 363, 1178, 1471, 1587, 1698, 1791, 2116
HapII CCGG 8 cut(s) 15, 333, 344, 387, 402, 560, 666, 1118
HgaI GACGC 1 cut(s) 515
HhaI GCGC 4 cut(s) 836, 1075, 1077, 2154
Hin6I GCGC 4 cut(s) 834, 1073, 1075, 2152
HinP1I GCGC 4 cut(s) 834, 1073, 1075, 2152
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HindIII AAGCTT 1 cut(s) 1683
HpaII CCGG 8 cut(s) 15, 333, 344, 387, 402, 560, 666, 1118
Hpy166II GTNNAC 1 cut(s) 1161
Hpy188III TCNNGA 5 cut(s) 292, 530, 666, 1345, 1454
Hpy8I GTNNAC 1 cut(s) 1161
HpyCH4III ACNGT 5 cut(s) 205, 586, 658, 1956, 2033
HpyCH4IV ACGT 3 cut(s) 179, 222, 354
HpyF3I CTNAG 3 cut(s) 673, 1773, 2124
HpySE526I ACGT 3 cut(s) 179, 222, 354
HspAI GCGC 4 cut(s) 834, 1073, 1075, 2152
Kpn2I TCCGGA 1 cut(s) 665
Ksp22I TGATCA 2 cut(s) 940, 1632
LmnI GCTCC 4 cut(s) 341, 1120, 1630, 1914
Lsp1109I GCAGC 6 cut(s) 20, 1193, 1425, 1937, 2008, 2166
LweI GCATC 7 cut(s) 85, 146, 500, 1046, 1104, 1291, 2156
MaeII ACGT 3 cut(s) 179, 222, 354
MaeIII GTNAC 3 cut(s) 223, 574, 826
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 3 cut(s) 21, 1085, 1602
MlsI TGGCCA 1 cut(s) 1791
MluCI AATT 8 cut(s) 325, 469, 483, 542, 644, 807, 1581, 1763
MluNI TGGCCA 1 cut(s) 1791
MlyI GAGTC 9 cut(s) 304, 431, 640, 1037, 1099, 1318, 1342, 1743, 2135
MmeI TCCRAC 1 cut(s) 441
Mox20I TGGCCA 1 cut(s) 1791
Mph1103I ATGCAT 1 cut(s) 139
MroI TCCGGA 1 cut(s) 665
MscI TGGCCA 1 cut(s) 1791
MseI TTAA 4 cut(s) 213, 273, 1464, 1481
MslI CAYNNNNRTG 4 cut(s) 431, 1338, 1711, 1920
Msp20I TGGCCA 1 cut(s) 1791
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 8 cut(s) 15, 333, 344, 387, 402, 560, 666, 1118
MspR9I CCNGG 4 cut(s) 16, 344, 388, 560
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 1 cut(s) 1282
MvnI CGCG 3 cut(s) 836, 1075, 2152
NciI CCSGG 4 cut(s) 16, 344, 388, 560
NcoI CCATGG 2 cut(s) 1552, 1786
NlaIV GGNNCC 6 cut(s) 159, 202, 337, 364, 919, 1588
NmeAIII GCCGAG 2 cut(s) 1049, 1210
NsiI ATGCAT 1 cut(s) 139
NspI RCATGY 1 cut(s) 1658
NspV TTCGAA 3 cut(s) 301, 782, 1978
OliI CACNNNNGTG 2 cut(s) 1711, 1920
PaeR7I CTCGAG 1 cut(s) 530
PauI GCGCGC 1 cut(s) 1073
PctI GAATGC 1 cut(s) 1282
PfeI GAWTC 4 cut(s) 149, 391, 511, 622
PfoI TCCNGGA 3 cut(s) 342, 386, 558
PkrI GCNGC 9 cut(s) 7, 10, 13, 1180, 1183, 1415, 1927, 2023, 2156
PleI GAGTC 9 cut(s) 303, 431, 639, 1036, 1098, 1318, 1342, 1742, 2134
PpsI GAGTC 9 cut(s) 303, 431, 639, 1036, 1098, 1318, 1342, 1742, 2134
Psp1406I AACGTT 1 cut(s) 222
PspN4I GGNNCC 6 cut(s) 159, 202, 337, 364, 919, 1588
PspPI GGNCC 4 cut(s) 362, 460, 586, 1586
PstI CTGCAG 3 cut(s) 10, 519, 639
PstNI CAGNNNCTG 2 cut(s) 523, 2123
PsuI RGATCY 1 cut(s) 408
PteI GCGCGC 1 cut(s) 1073
PvuII CAGCTG 1 cut(s) 11
RseI CAYNNNNRTG 4 cut(s) 431, 1338, 1711, 1920
SaqAI TTAA 4 cut(s) 213, 273, 1464, 1481
SatI GCNGC 9 cut(s) 6, 9, 12, 1179, 1182, 1414, 1926, 2022, 2155
Sau96I GGNCC 4 cut(s) 362, 460, 586, 1586
ScaI AGTACT 1 cut(s) 1063
SchI GAGTC 9 cut(s) 304, 431, 640, 1037, 1099, 1318, 1342, 1743, 2135
ScrFI CCNGG 4 cut(s) 16, 344, 388, 560
SduI GDGCHC 3 cut(s) 21, 1085, 1602
SfaNI GCATC 7 cut(s) 85, 146, 500, 1046, 1104, 1291, 2156
SfcI CTRYAG 4 cut(s) 6, 515, 635, 1366
Sfr274I CTCGAG 1 cut(s) 530
SfuI TTCGAA 3 cut(s) 301, 782, 1978
SinI GGWCC 2 cut(s) 460, 586
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 4 cut(s) 431, 1338, 1711, 1920
SmlI CTYRAG 2 cut(s) 110, 530
SmoI CTYRAG 2 cut(s) 110, 530
SpeI ACTAGT 1 cut(s) 2009
Sse9I AATT 8 cut(s) 325, 469, 483, 542, 644, 807, 1581, 1763
StyD4I CCNGG 4 cut(s) 14, 342, 386, 558
StyI CCWWGG 3 cut(s) 1552, 1786, 1792
TaaI ACNGT 5 cut(s) 205, 586, 658, 1956, 2033
TaiI ACGT 3 cut(s) 182, 225, 357
TaqI TCGA 8 cut(s) 86, 285, 301, 531, 776, 782, 1088, 1978
TasI AATT 8 cut(s) 325, 469, 483, 542, 644, 807, 1581, 1763
TatI WGTACW 4 cut(s) 707, 1061, 1398, 1576
TauI GCSGC 1 cut(s) 1181
TfiI GAWTC 4 cut(s) 149, 391, 511, 622
Tru1I TTAA 4 cut(s) 213, 273, 1464, 1481
Tru9I TTAA 4 cut(s) 213, 273, 1464, 1481
TscAI CASTG 3 cut(s) 481, 553, 1900
TseI GCWGC 8 cut(s) 5, 8, 11, 1181, 1413, 1925, 2021, 2154
TspDTI ATGAA 6 cut(s) 555, 1152, 1187, 1203, 1289, 1542
TspGWI ACGGA 1 cut(s) 1178
TspRI CASTG 3 cut(s) 481, 553, 1900
VpaK11BI GGWCC 2 cut(s) 460, 586
XapI RAATTY 4 cut(s) 483, 542, 644, 1763
XceI RCATGY 1 cut(s) 1658
XcmI CCANNNNNNNNNTGG 1 cut(s) 1793
XhoI CTCGAG 1 cut(s) 530
ZrmI AGTACT 1 cut(s) 1063
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.