Rw2G036780
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018061
Physical Location & Seq
Reverse (-)
60571749 .. 60574868
3120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G036780.1

Sequence Viewer

Length: 2223 bp
ATGGCTGCAGCTGCCGGGCTCAATCTTCACCGCCCGTACCTCCTCCTCTCCATAATCTTCTTCTCCCTTCCTCCATTAACATATTCGATAACCGAAGCCGAAGCGTTGCTCAAGTTCAAGCAATCCCTCACCAATGCAAACGCTTTGGATTCTTGGGTCCCTAACTCTGCACCATGCACCAAAGATGCGCAATGGGCCGGACTCGTATGCTTTAATGGGATCGTTACGGGTCTTCGTCTCGGGGGAATGGGGTTATCTGGTAACCTAGACTTTGAATCGCTGTATGAAGTCAAGGGTCTTCGAACCATGAGCTTGGTGGACAACAATTTCTCCGGCTCCATCCCTAATTTCAACGTCATTGGCCCCTTGAAAGCTATCTATTTGTCCAGGAATGGGTTTTCCGGCGAGATCCCATCGGACTTTTTCACCAACATGGACTCATTGAAGAAAGTGTGGCTGGACCAAAATCAATTCAGCGGGAAAATTCCGGTTTCTCTGGCGGAAAGTCCCAATCTGCAGGTACTGCATCTCGAGAACAATCAGTTCAGCGGGAGCCTCCCGGAAATGAACCAGAAGTCGTTGAACACCCTCAATGTGTCTAATAACAAGCTGGAGGGGGAGATTCCGAAGAGTTTGGCTAAATTTGATGAGGCTGTGTTTTCTGGAAACTCAGGTCTTTGTGGGCAAAAGGTGGGCAAAGAATGCAAAAATGTAGCAGGGGAGCCAACACCATCACCCTCACCCTCACCCTCACCAACGAGTTCTAATGACAGCAATGAGGGTAGTGGCTCGAAATCGAGTTCGAAAAGTAAGGGGGGAAGCTCTACAATTGTTATTGCATTGTCAGTAACTTGCGCGGTGGTGCTTCTCATTCTTATCCTTCTGTTGTTTGTCAGATCAAGGAGGAAGAAGGCAGATGAGAATTTCGATACTCTTGGAACCCAAAATAGTCAAAGCACTGATCATTCTCCTCCTCAAGAAAGAAACGTAGAAGCAGTTGAGTTGAATGTGGCGGAGATAAATAACAAGGAAAGGGAAGTGGTAGTTGAGCCATCTCCATCAGTTGGTAGTGCTAAAAAGGGCGGCTCGGAGCGCAAGAGTACTTCCAGTCATGCTAGAGGAGGCTGCGGGATGGGCGATCTAGTGATGTTGAGTGAAGAGAAGGGAGTGTTTGGGTTGACGGATTTGATGAAGGCCGCGGCAGAGGTGCTTGGCAATGGGGGGATGGGTTCGTCGTACAAGGCTGTCATGGCGAATGGGTTGGCGGTTGTGGTGAAACGGATAAGAGAAATGAATGCAATGGGGAGAGAGGGCTTTGATGCAGAGATGAGGAGGTATGGGACGCTACGTCATTGGAATGTCTTGACTCCTTTGGCTTATCACTACAGAAAAGAAGAGAAGCTGTTAATCTATGAGTACATCCCCAAGAGCAGCTTGCTTTATCTGTTACATGGTGATCGTGGACCGTCTCACGACCAACTTCACTGGCATGCTAGGTTAAAGATTATTCAAGGCACGGCAAGAGGGATGGGTTTTATCCATGCTGAATTTTCAGCCTATGATTTGCCCCATGGGGACCTAAAGTCCAGCAATGTACTAATTGGCCCCGACTATGAGCCATTGCTTTCAGACTTTGCATTTGCTCCAATGATCAACTCTTCACATTTGCCACAAGCCTTGTTTGCATACAAAGCCCCAGAAGCTTTAGAATTTGGCCAAGTATCACCCAAGTGCGATGTGTTTTGTCTAGGAGTCCTCATTCTCGAAACCCTAACGGGCAAATTCCCTTCTCAGTATCTCAACAATGGCAAAGGTGGGATTGATGTAGTCCAATGGGTGCGGTCAGCTATATCTGAAAGGAGAGAATTGGAACTATTAGACCCTGAGATTTCAAACGAAAAGGGTTCAATCAGTGAAATGGAGAAGCTCCTCCACATTGGTGCAGCCTGCACGGAGAATGATCCAAGGCAACGGTTAGATATGTTGGAGGCTATTCGAAGGATAGAGCAGATAAAGGCCATCGAAACTAGTGAAGACGCTGCTAGAACAATACAAGTGCTACCGTCACTTAGAGACGGTTGTGCAGACTCTTCTTTAGTAATGCCAAAGACAACTCATGCACCGACATTGCGGGAGGGGGTAGCAGATATCCACGCACCTGCAGCTGCTGAGACCGAGATTAGCAGACTGCCATCAAAGAGCTTCCGAAGCAAATCGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

740

Amino Acids

80.3

Weight (kDa)

6.29

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 70 3.5e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 123 - 182 2e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 401 - 610 1.2e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 402 - 665 4.8e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018061)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35120
prunus_persica Prupe.3G037900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0146351
rosa_laevigata RLG00000020196
rosa_multiflora Rmu_sc0002054.1_g000009
rosa_rugosa Rorug02G0394100
rosa_samantha Rh2AG450600 Rh2BG463000 Rh2CG437400 Rh2DG472000
rosa_wichuraiana Rw2G036780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2167
Acc16I TGCGCA 1 cut(s) 189
Acc36I ACCTGC 2 cut(s) 508, 2167
AccB7I CCANNNNNTGG 1 cut(s) 1064
AccII CGCG 2 cut(s) 857, 1199
AclWI GGATC 3 cut(s) 227, 403, 1955
AcoI YGGCCR 1 cut(s) 1714
AcsI RAATTY 6 cut(s) 483, 641, 922, 1547, 1709, 1781
AfaI GTAC 6 cut(s) 38, 522, 1102, 1238, 1418, 1596
AfiI CCNNNNNNNGG 2 cut(s) 393, 1064
AhdI GACNNNNNGTC 2 cut(s) 1347, 1582
AhlI ACTAGT 1 cut(s) 2027
AjnI CCWGG 1 cut(s) 386
AleI CACNNNNGTG 2 cut(s) 1729, 1938
Alw26I GTCTC 4 cut(s) 242, 1473, 2067, 2165
AlwI GGATC 3 cut(s) 227, 403, 1955
AlwNI CAGNNNCTG 2 cut(s) 523, 2168
Ama87I CYCGRG 2 cut(s) 239, 530
AoxI GGCC 6 cut(s) 195, 361, 1194, 1603, 1714, 2016
ApeKI GCWGC 9 cut(s) 5, 8, 11, 1125, 1431, 1943, 2039, 2162, 2165
ApoI RAATTY 6 cut(s) 483, 641, 922, 1547, 1709, 1781
ArsI GACNNNNNNTTYG 2 cut(s) 1622, 1654
AspLEI GCGC 3 cut(s) 190, 857, 1095
AspS9I GGNCC 7 cut(s) 157, 195, 362, 460, 1463, 1576, 1604
AsuC2I CCSGG 2 cut(s) 16, 560
AsuII TTCGAA 3 cut(s) 301, 803, 1996
AvaI CYCGRG 2 cut(s) 239, 530
AvaII GGWCC 4 cut(s) 157, 460, 1463, 1576
BalI TGGCCA 1 cut(s) 1716
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 3 cut(s) 224, 290, 2040
BbvI GCAGC 7 cut(s) 20, 1112, 1443, 1955, 2026, 2152, 2174
BceAI ACGGC 1 cut(s) 1533
BciT130I CCWGG 1 cut(s) 388
BclI TGATCA 2 cut(s) 961, 1650
BcnI CCSGG 2 cut(s) 16, 560
BcoDI GTCTC 4 cut(s) 242, 1473, 2067, 2165
BcuI ACTAGT 1 cut(s) 2027
BfaI CTAG 7 cut(s) 266, 1116, 1142, 1494, 1748, 2028, 2043
BfmI CTRYAG 4 cut(s) 6, 515, 1384, 2160
BfuAI ACCTGC 2 cut(s) 508, 2167
BmcAI AGTACT 1 cut(s) 1102
Bme1390I CCNGG 3 cut(s) 16, 388, 560
Bme18I GGWCC 4 cut(s) 157, 460, 1463, 1576
BmeRI GACNNNNNGTC 2 cut(s) 1347, 1582
BmeT110I CYCGRG 2 cut(s) 239, 530
BmgT120I GGNCC 7 cut(s) 157, 195, 362, 460, 1463, 1576, 1604
BmiI GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 940, 1577, 1606
BmrFI CCNGG 3 cut(s) 16, 388, 560
BmsI GCATC 3 cut(s) 175, 535, 1309
BpiI GAAGAC 3 cut(s) 224, 290, 2040
BpmI CTGGAG 1 cut(s) 632
Bpu14I TTCGAA 3 cut(s) 301, 803, 1996
BpuEI CTTGAG 2 cut(s) 95, 960
BpuMI CCSGG 2 cut(s) 16, 560
BsaI GGTCTC 1 cut(s) 2165
BsaJI CCNNGG 3 cut(s) 1197, 1570, 1964
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 1064
Bse1I ACTGG 2 cut(s) 1107, 1490
Bse3DI GCAATG 7 cut(s) 197, 781, 1222, 1305, 1597, 1619, 2126
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 1197, 1570, 1964
BseGI GGATG 5 cut(s) 339, 1137, 1230, 1419, 1533
BseLI CCNNNNNNNGG 2 cut(s) 393, 1064
BseMI GCAATG 7 cut(s) 197, 781, 1222, 1305, 1597, 1619, 2126
BseMII CTCAG 4 cut(s) 684, 1805, 1875, 2160
BseNI ACTGG 2 cut(s) 1107, 1490
BseRI GAGGAG 7 cut(s) 32, 35, 960, 963, 1134, 1345, 1919
BseXI GCAGC 7 cut(s) 20, 1112, 1443, 1955, 2026, 2152, 2174
BsgI GTGCAG 4 cut(s) 153, 1933, 1962, 2103
Bsh1236I CGCG 2 cut(s) 857, 1199
BshFI GGCC 6 cut(s) 197, 363, 1196, 1605, 1716, 2018
BsiHKCI CYCGRG 2 cut(s) 239, 530
BsiSI CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
BslFI GGGAC 4 cut(s) 143, 492, 1354, 1589
BslI CCNNNNNNNGG 2 cut(s) 393, 1064
BsmAI GTCTC 4 cut(s) 242, 1473, 2067, 2165
BsmBI CGTCTC 3 cut(s) 242, 1473, 2067
BsmFI GGGAC 4 cut(s) 143, 492, 1354, 1589
BsmI GAATGC 2 cut(s) 707, 1300
BsnI GGCC 6 cut(s) 197, 363, 1196, 1605, 1716, 2018
Bso31I GGTCTC 1 cut(s) 2165
BsoBI CYCGRG 2 cut(s) 239, 530
Bsp119I TTCGAA 3 cut(s) 301, 803, 1996
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
Bsp19I CCATGG 1 cut(s) 1570
BspANI GGCC 6 cut(s) 197, 363, 1196, 1605, 1716, 2018
BspCNI CTCAG 4 cut(s) 683, 1804, 1876, 2161
BspFNI CGCG 2 cut(s) 857, 1199
BspLI GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 940, 1577, 1606
BspMAI CTGCAG 3 cut(s) 10, 519, 2164
BspMI ACCTGC 2 cut(s) 508, 2167
BspPI GGATC 3 cut(s) 227, 403, 1955
BspT104I TTCGAA 3 cut(s) 301, 803, 1996
BspTNI GGTCTC 1 cut(s) 2165
BsrDI GCAATG 7 cut(s) 197, 781, 1222, 1305, 1597, 1619, 2126
BsrI ACTGG 2 cut(s) 1107, 1490
BssECI CCNNGG 3 cut(s) 1197, 1570, 1964
BssMI GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
BssT1I CCWWGG 2 cut(s) 1570, 1964
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 4 cut(s) 1467, 1974, 2064, 2078
Bst6I CTCTTC 5 cut(s) 623, 1152, 1389, 1663, 2095
BstAPI GCANNNNNTGC 2 cut(s) 523, 702
BstBI TTCGAA 3 cut(s) 301, 803, 1996
BstC8I GCNNGC 3 cut(s) 1436, 1491, 1948
BstDEI CTNAG 5 cut(s) 670, 1791, 1884, 2069, 2169
BstDSI CCRYGG 2 cut(s) 1197, 1570
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 5 cut(s) 339, 1137, 1230, 1419, 1533
BstFNI CGCG 2 cut(s) 857, 1199
BstHHI GCGC 3 cut(s) 190, 857, 1095
BstKTI GATC 8 cut(s) 222, 411, 899, 964, 1141, 1459, 1653, 1963
BstMAI GTCTC 4 cut(s) 242, 1473, 2067, 2165
BstMBI GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
BstNI CCWGG 1 cut(s) 388
BstNSI RCATGY 1 cut(s) 1493
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 14, 386, 558
BstSFI CTRYAG 4 cut(s) 6, 515, 1384, 2160
BstUI CGCG 2 cut(s) 857, 1199
BstV1I GCAGC 7 cut(s) 20, 1112, 1443, 1955, 2026, 2152, 2174
BstV2I GAAGAC 3 cut(s) 224, 290, 2040
BstX2I RGATCY 1 cut(s) 408
BstXI CCANNNNNNTGG 1 cut(s) 313
BstYI RGATCY 1 cut(s) 408
BsuRI GGCC 6 cut(s) 197, 363, 1196, 1605, 1716, 2018
BtgI CCRYGG 2 cut(s) 1197, 1570
BtgZI GCGATG 1 cut(s) 1749
BtsCI GGATG 5 cut(s) 339, 1137, 1230, 1419, 1533
BtsIMutI CAGTG 3 cut(s) 957, 1483, 1918
BveI ACCTGC 2 cut(s) 508, 2167
Cac8I GCNNGC 3 cut(s) 1436, 1491, 1948
CaiI CAGNNNCTG 2 cut(s) 523, 2168
CfoI GCGC 3 cut(s) 190, 857, 1095
Cfr13I GGNCC 7 cut(s) 157, 195, 362, 460, 1463, 1576, 1604
Cfr42I CCGCGG 1 cut(s) 1200
CseI GACGC 2 cut(s) 1351, 2045
Csp6I GTAC 6 cut(s) 37, 521, 1101, 1237, 1417, 1595
CspCI CAANNNNNGTGG 2 cut(s) 1659, 1694
CviQI GTAC 6 cut(s) 37, 521, 1101, 1237, 1417, 1595
DdeI CTNAG 5 cut(s) 670, 1791, 1884, 2069, 2169
DpnI GATC 8 cut(s) 221, 410, 898, 963, 1140, 1458, 1652, 1962
DpnII GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
DriI GACNNNNNGTC 2 cut(s) 1347, 1582
EaeI YGGCCR 1 cut(s) 1714
Eam1104I CTCTTC 5 cut(s) 623, 1152, 1389, 1663, 2095
Eam1105I GACNNNNNGTC 2 cut(s) 1347, 1582
EarI CTCTTC 5 cut(s) 623, 1152, 1389, 1663, 2095
EciI GGCGGA 2 cut(s) 515, 1028
Eco130I CCWWGG 2 cut(s) 1570, 1964
Eco24I GRGCYC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 2165
Eco32I GATATC 1 cut(s) 2149
Eco47I GGWCC 4 cut(s) 157, 460, 1463, 1576
Eco88I CYCGRG 2 cut(s) 239, 530
Eco91I GGTNACC 1 cut(s) 260
EcoO109I RGGNCCY 2 cut(s) 157, 1576
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 386
EcoRV GATATC 1 cut(s) 2149
EcoT14I CCWWGG 2 cut(s) 1570, 1964
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 2 cut(s) 1570, 1964
Esp3I CGTCTC 3 cut(s) 242, 1473, 2067
FalI AAGNNNNNCTT 2 cut(s) 1418, 1450
FaqI GGGAC 4 cut(s) 143, 492, 1354, 1589
FauI CCCGC 4 cut(s) 470, 542, 1121, 2124
FbaI TGATCA 2 cut(s) 961, 1650
FokI GGATG 5 cut(s) 326, 1144, 1237, 1406, 1540
FriOI GRGCYC 1 cut(s) 21
FspBI CTAG 7 cut(s) 266, 1116, 1142, 1494, 1748, 2028, 2043
FspI TGCGCA 1 cut(s) 189
GlaI GCGC 3 cut(s) 189, 856, 1094
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 6 cut(s) 197, 363, 1196, 1605, 1716, 2018
HapII CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
HgaI GACGC 2 cut(s) 1351, 2045
HhaI GCGC 3 cut(s) 190, 857, 1095
Hin6I GCGC 3 cut(s) 188, 855, 1093
HinP1I GCGC 3 cut(s) 188, 855, 1093
HincII GTYRAC 1 cut(s) 1179
HindII GTYRAC 1 cut(s) 1179
HindIII AAGCTT 1 cut(s) 1701
HinfI GANTC 8 cut(s) 149, 201, 275, 437, 622, 1366, 1752, 2087
HpaII CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
Hpy166II GTNNAC 3 cut(s) 319, 1179, 1463
Hpy188I TCNGA 7 cut(s) 418, 627, 896, 1090, 1630, 1855, 2207
Hpy188III TCNNGA 7 cut(s) 530, 532, 663, 977, 1363, 1472, 1763
Hpy8I GTNNAC 3 cut(s) 319, 1179, 1463
Hpy99I CGWCG 1 cut(s) 1237
HpyAV CCTTC 7 cut(s) 77, 890, 904, 1156, 1186, 1797, 1992
HpyCH4III ACNGT 4 cut(s) 1467, 1974, 2064, 2078
HpyCH4IV ACGT 3 cut(s) 354, 987, 1348
HpyF3I CTNAG 5 cut(s) 670, 1791, 1884, 2069, 2169
HpySE526I ACGT 3 cut(s) 354, 987, 1348
HspAI GCGC 3 cut(s) 188, 855, 1093
KflI GGGWCCC 1 cut(s) 157
Ksp22I TGATCA 2 cut(s) 961, 1650
KspI CCGCGG 1 cut(s) 1200
Kzo9I GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
LmnI GCTCC 6 cut(s) 341, 552, 721, 1090, 1648, 1932
Lsp1109I GCAGC 7 cut(s) 20, 1112, 1443, 1955, 2026, 2152, 2174
LweI GCATC 3 cut(s) 175, 535, 1309
MaeI CTAG 7 cut(s) 266, 1116, 1142, 1494, 1748, 2028, 2043
MaeII ACGT 3 cut(s) 354, 987, 1348
MaeIII GTNAC 5 cut(s) 223, 260, 847, 1446, 2064
MalI GATC 8 cut(s) 221, 410, 898, 963, 1140, 1458, 1652, 1962
MboI GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
MfeI CAATTG 1 cut(s) 828
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 1716
MluNI TGGCCA 1 cut(s) 1716
MlyI GAGTC 5 cut(s) 195, 431, 1360, 1761, 2081
MmeI TCCRAC 1 cut(s) 1965
Mox20I TGGCCA 1 cut(s) 1716
MscI TGGCCA 1 cut(s) 1716
MseI TTAA 4 cut(s) 77, 213, 1406, 1499
MslI CAYNNNNRTG 5 cut(s) 431, 1356, 1488, 1729, 1938
Msp20I TGGCCA 1 cut(s) 1716
MspA1I CMGCKG 5 cut(s) 11, 477, 549, 1199, 2165
MspI CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
MspR9I CCNGG 3 cut(s) 16, 388, 560
MunI CAATTG 1 cut(s) 828
Mva1269I GAATGC 2 cut(s) 707, 1300
MvaI CCWGG 1 cut(s) 388
MvnI CGCG 2 cut(s) 857, 1199
NciI CCSGG 2 cut(s) 16, 560
NcoI CCATGG 1 cut(s) 1570
NdeII GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
NlaIV GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 940, 1577, 1606
NmuCI GTSAC 1 cut(s) 2064
NsbI TGCGCA 1 cut(s) 189
NspI RCATGY 1 cut(s) 1493
NspV TTCGAA 3 cut(s) 301, 803, 1996
OliI CACNNNNGTG 2 cut(s) 1729, 1938
PaeI GCATGC 1 cut(s) 1493
PaeR7I CTCGAG 1 cut(s) 530
PaqCI CACCTGC 1 cut(s) 2167
PctI GAATGC 2 cut(s) 707, 1300
PfeI GAWTC 3 cut(s) 149, 275, 622
PflMI CCANNNNNTGG 1 cut(s) 1064
PfoI TCCNGGA 2 cut(s) 386, 558
PleI GAGTC 5 cut(s) 195, 431, 1360, 1760, 2081
PpsI GAGTC 5 cut(s) 195, 431, 1360, 1760, 2081
PpuMI RGGWCCY 2 cut(s) 157, 1576
Psp5II RGGWCCY 2 cut(s) 157, 1576
Psp6I CCWGG 1 cut(s) 386
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 940, 1577, 1606
PspPI GGNCC 7 cut(s) 157, 195, 362, 460, 1463, 1576, 1604
PspPPI RGGWCCY 2 cut(s) 157, 1576
PstI CTGCAG 3 cut(s) 10, 519, 2164
PstNI CAGNNNCTG 2 cut(s) 523, 2168
PsuI RGATCY 1 cut(s) 408
PvuII CAGCTG 2 cut(s) 11, 2165
RsaI GTAC 6 cut(s) 38, 522, 1102, 1238, 1418, 1596
RsaNI GTAC 6 cut(s) 37, 521, 1101, 1237, 1417, 1595
RseI CAYNNNNRTG 5 cut(s) 431, 1356, 1488, 1729, 1938
SacII CCGCGG 1 cut(s) 1200
SaqAI TTAA 4 cut(s) 77, 213, 1406, 1499
Sau3AI GATC 8 cut(s) 219, 408, 896, 961, 1138, 1456, 1650, 1960
Sau96I GGNCC 7 cut(s) 157, 195, 362, 460, 1463, 1576, 1604
ScaI AGTACT 1 cut(s) 1102
SchI GAGTC 5 cut(s) 195, 431, 1360, 1761, 2081
ScrFI CCNGG 3 cut(s) 16, 388, 560
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 3 cut(s) 175, 535, 1309
SfcI CTRYAG 4 cut(s) 6, 515, 1384, 2160
Sfr274I CTCGAG 1 cut(s) 530
Sfr303I CCGCGG 1 cut(s) 1200
SfuI TTCGAA 3 cut(s) 301, 803, 1996
SgrBI CCGCGG 1 cut(s) 1200
SinI GGWCC 4 cut(s) 157, 460, 1463, 1576
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 5 cut(s) 431, 1356, 1488, 1729, 1938
SmlI CTYRAG 3 cut(s) 110, 530, 975
SmoI CTYRAG 3 cut(s) 110, 530, 975
SpeI ACTAGT 1 cut(s) 2027
SphI GCATGC 1 cut(s) 1493
SspMI CTAG 7 cut(s) 266, 1116, 1142, 1494, 1748, 2028, 2043
StyD4I CCNGG 3 cut(s) 14, 386, 558
StyI CCWWGG 2 cut(s) 1570, 1964
TaaI ACNGT 4 cut(s) 1467, 1974, 2064, 2078
TaiI ACGT 3 cut(s) 357, 990, 1351
TaqII GACCGA 1 cut(s) 2189
TatI WGTACW 3 cut(s) 1100, 1416, 1594
TauI GCSGC 3 cut(s) 1086, 1199, 1202
TfiI GAWTC 3 cut(s) 149, 275, 622
Tru1I TTAA 4 cut(s) 77, 213, 1406, 1499
Tru9I TTAA 4 cut(s) 77, 213, 1406, 1499
TscAI CASTG 3 cut(s) 964, 1490, 1918
TseFI GTSAC 1 cut(s) 2064
TseI GCWGC 9 cut(s) 5, 8, 11, 1125, 1431, 1943, 2039, 2162, 2165
Tsp45I GTSAC 1 cut(s) 2064
TspDTI ATGAA 4 cut(s) 300, 581, 1205, 1307
TspGWI ACGGA 3 cut(s) 1196, 1294, 1967
TspRI CASTG 3 cut(s) 964, 1490, 1918
Van91I CCANNNNNTGG 1 cut(s) 1064
VpaK11BI GGWCC 4 cut(s) 157, 460, 1463, 1576
XapI RAATTY 6 cut(s) 483, 641, 922, 1547, 1709, 1781
XceI RCATGY 1 cut(s) 1493
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 7 cut(s) 266, 1116, 1142, 1494, 1748, 2028, 2043
ZrmI AGTACT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.