Rh2DG472000
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018061
Physical Location & Seq
Reverse (-)
68385720 .. 68389354
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG472000.1

Sequence Viewer

Length: 2217 bp
ATGGCTGCAGCTGCCGGGCTCAATCTTCACCGCCCGTACCTCCTCCTCTCCATAATCTTCTTCTCCCTTCCTCCATTAACATATTCGATAACCGAAGCCGAAGCGTTGCTCAAGTTCAAGCAATCCCTCACCAATGCAAACGCTTTGGATTCTTGGGTCCCTAACTCTGCACCATGCACCAAAGATGCGCAATGGGCCGGACTCGTATGCTTTAATGGGATCGTTACGGGTCTTCGTCTCGGGGGAATGGGGTTATCTGGTAACCTAGACTTTGAATCGCTGTATGAAGTCAAGGGTCTTCGAACCATGAGCTTGGTGGACAACAATTTCTCCGGCTCCATCCCTAATTTCAACGTCATTGGCCCCTTGAAAGCTATCTATTTGTCCAGGAATGGGTTTTCCGGCGAGATCCCATCGGACTTTTTCACCAACATGGACTCATTGAAGAAAGTGTGGCTGGACCAAAATCAATTCAGCGGGAAAATTCCAGTTTCTCTGGCGGAAAGTCCCAATCTGCAGGTACTGCATCTCGAGAACAATCAGTTCAGCGGGAGCCTCCCGGAAATGAACCAGAAGTCGTTGAACACCCTCAATGTGTCTAATAACAAGCTGGAGGGGGAGATTCCGAAGAGTTTGGCTAAATTTGATGAGGCTGTGTTTTCTGGAAACTCAGGTCTTTGTGGGCAAAAGGTGGGCAAAGAATGCAAAAATGTAGCAGGGGAGCCAACACCATCACCCTCACCCTCACCAACGAGTTCTAATGACAGCAATGAGGGTAGTGGCTCGAAATCGAGTTCGAAAAGTAAGGGGGGAAGCTCTACAATTGTTATTGCATTGTCAGTAACTTGCGCGGTGGTGCTTCTCATTCTTATCCTTCTGTTGTTTGTCAGATCAAGGAGGAAGAAGGCAGATGAGAATTTCGATACTCTTGGAACCCAAAATAGTCAAAGCACTGATCATTCTCCTCCTCAAGAAAGAAACGTAGAAGCAGTTGAGTTGAATGTGGCGGAGATAAATAACAAGGAAAGGGAAGTGGTAGTTGAGCCATCTCCATCAGTTGGTAGTGCTAAAAAGGGTGGCTCGGAGCGCAAGAGTACTTCCAGTCATGCTAGAGGAGGCTGCGGGATGGGCGATCTAGTGATGTTGAGTGAAGAGAAGGGAGTGTTTGGGTTGACGGATTTGATGAAGGCCGCGGCAGAGGTGCTTGGCAATGGGGGGATGGGTTCGTCGTACAAGGCTGTCATGGCGAATGGGTTGGCGGTTGTGGTGAAACGGATAAGAGAAATGAATGCAATGGGGAGAGAGGGCTTTGATGCGGAGATGAGGAGGTATGGGACGCTACGTCATTGGAATGTCTTGACTCCTTTGGCTTATCACTACAGAAAAGAAGAGAAGCTGTTAATCTATGAGTACATCCCCAAGAGCAGCTTGCTTTATCTGTTACATGGTGATCGCGGACCGTCTCACGACCAACTTAACTGGCATGCTAGGTTAAAGATTATTCAAGGCACGGCAAGAGGGATGGGTTTTATCCATGCTGAATTTTCAGCCTATGATTTGCCCCATGGGGACCTAAAGTCCAGCAATGTACTAATTGGCCCCGACTATGAGCCATTGCTTTCAGACTTTGCATTTGCTCCAATGATCAACTCTTCACATTTGCCACAAGCCTTGTTTGCATACAAAGCCCCAGAAGCTTTAGAATTTGGCCAAGTATCACCCAAGTGCGATGTGTTTTGTCTAGGAGTCCTCATTCTCGAAACCCTAACGGGCAAATTCCCTTCTCAGTATCTCAACAATGGCAAAGGTGGGATTGATGTAGTCCAATGGGTGCGGTCGGCTATATCTGAAAGGAGAGAATTGGAACTATTAGACCCTGAGATTTCAAACGAAAAGGGTTCAATCAGTGAAATGGAGAAGCTCCTCCACATTGGTGCAGCCTGTACGGAGAATGATCCAAGGCAACGGTTAGATATGTTGGAGGCTATTCGAAGGATAGAGCAGATAAAGGCCATCGAAACTAGTGAAGACGCTGCTAGAACAATACAAGTGCTACCGTCACTTAGAGACGGTTGTGCAGACTCTTCTTTAGTAATGCCAAAGACAACTCATGCACCGACATTGCGGGAGGGGGTAGCAGATATCCACGCACCTGCAGCTGCTGAGACCGAGATTAGCAGACTGCCATCAAAGAGCTTCCGAAGCAAATCGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

738

Amino Acids

80.09

Weight (kDa)

6.26

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 70 3.4e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 123 - 182 2e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 399 - 608 7.8e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 400 - 663 4.5e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018061)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35120
prunus_persica Prupe.3G037900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0146351
rosa_laevigata RLG00000020196
rosa_multiflora Rmu_sc0002054.1_g000009
rosa_rugosa Rorug02G0394100
rosa_samantha Rh2AG450600 Rh2BG463000 Rh2CG437400 Rh2DG472000
rosa_wichuraiana Rw2G036780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2161
Acc16I TGCGCA 1 cut(s) 189
Acc36I ACCTGC 2 cut(s) 508, 2161
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccII CGCG 3 cut(s) 851, 1193, 1455
AclWI GGATC 3 cut(s) 227, 403, 1949
AcoI YGGCCR 1 cut(s) 1708
AcsI RAATTY 6 cut(s) 483, 641, 916, 1541, 1703, 1775
AfaI GTAC 7 cut(s) 38, 522, 1096, 1232, 1412, 1590, 1945
AfiI CCNNNNNNNGG 2 cut(s) 393, 1058
AhdI GACNNNNNGTC 2 cut(s) 1341, 1576
AhlI ACTAGT 1 cut(s) 2021
AjnI CCWGG 1 cut(s) 386
AleI CACNNNNGTG 2 cut(s) 1723, 1932
Alw26I GTCTC 4 cut(s) 242, 1467, 2061, 2159
AlwI GGATC 3 cut(s) 227, 403, 1949
AlwNI CAGNNNCTG 2 cut(s) 523, 2162
Ama87I CYCGRG 2 cut(s) 239, 530
AoxI GGCC 6 cut(s) 195, 361, 1188, 1597, 1708, 2010
ApeKI GCWGC 9 cut(s) 5, 8, 11, 1119, 1425, 1937, 2033, 2156, 2159
ApoI RAATTY 6 cut(s) 483, 641, 916, 1541, 1703, 1775
ArsI GACNNNNNNTTYG 2 cut(s) 1616, 1648
AspLEI GCGC 3 cut(s) 190, 851, 1089
AspS9I GGNCC 7 cut(s) 157, 195, 362, 460, 1457, 1570, 1598
AsuC2I CCSGG 2 cut(s) 16, 560
AsuHPI GGTGA 9 cut(s) 20, 121, 418, 726, 732, 738, 1279, 1460, 1710
AsuII TTCGAA 3 cut(s) 301, 797, 1990
AvaI CYCGRG 2 cut(s) 239, 530
AvaII GGWCC 4 cut(s) 157, 460, 1457, 1570
BalI TGGCCA 1 cut(s) 1710
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 3 cut(s) 224, 290, 2034
BbvI GCAGC 7 cut(s) 20, 1106, 1437, 1949, 2020, 2146, 2168
BceAI ACGGC 1 cut(s) 1527
BciT130I CCWGG 1 cut(s) 388
BclI TGATCA 2 cut(s) 955, 1644
BcnI CCSGG 2 cut(s) 16, 560
BcoDI GTCTC 4 cut(s) 242, 1467, 2061, 2159
BcuI ACTAGT 1 cut(s) 2021
BfaI CTAG 7 cut(s) 266, 1110, 1136, 1488, 1742, 2022, 2037
BfmI CTRYAG 4 cut(s) 6, 515, 1378, 2154
BfuAI ACCTGC 2 cut(s) 508, 2161
BmcAI AGTACT 1 cut(s) 1096
Bme1390I CCNGG 3 cut(s) 16, 388, 560
Bme18I GGWCC 4 cut(s) 157, 460, 1457, 1570
BmeRI GACNNNNNGTC 2 cut(s) 1341, 1576
BmeT110I CYCGRG 2 cut(s) 239, 530
BmgT120I GGNCC 7 cut(s) 157, 195, 362, 460, 1457, 1570, 1598
BmiI GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 934, 1571, 1600
BmrFI CCNGG 3 cut(s) 16, 388, 560
BmsI GCATC 3 cut(s) 175, 535, 1303
BpiI GAAGAC 3 cut(s) 224, 290, 2034
BpmI CTGGAG 1 cut(s) 632
Bpu14I TTCGAA 3 cut(s) 301, 797, 1990
BpuEI CTTGAG 2 cut(s) 95, 954
BpuMI CCSGG 2 cut(s) 16, 560
BsaI GGTCTC 1 cut(s) 2159
BsaJI CCNNGG 3 cut(s) 1191, 1564, 1958
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 1058
Bse1I ACTGG 3 cut(s) 488, 1101, 1484
Bse3DI GCAATG 7 cut(s) 197, 775, 1216, 1299, 1591, 1613, 2120
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 1191, 1564, 1958
BseGI GGATG 5 cut(s) 339, 1131, 1224, 1413, 1527
BseLI CCNNNNNNNGG 2 cut(s) 393, 1058
BseMI GCAATG 7 cut(s) 197, 775, 1216, 1299, 1591, 1613, 2120
BseMII CTCAG 4 cut(s) 684, 1799, 1869, 2154
BseNI ACTGG 3 cut(s) 488, 1101, 1484
BseRI GAGGAG 7 cut(s) 32, 35, 954, 957, 1128, 1339, 1913
BseXI GCAGC 7 cut(s) 20, 1106, 1437, 1949, 2020, 2146, 2168
BsgI GTGCAG 3 cut(s) 153, 1956, 2097
Bsh1236I CGCG 3 cut(s) 851, 1193, 1455
Bsh1285I CGRYCG 1 cut(s) 1838
BshFI GGCC 6 cut(s) 197, 363, 1190, 1599, 1710, 2012
BsiEI CGRYCG 1 cut(s) 1838
BsiHKCI CYCGRG 2 cut(s) 239, 530
BsiSI CCGG 5 cut(s) 15, 198, 333, 402, 560
BslFI GGGAC 4 cut(s) 143, 492, 1348, 1583
BslI CCNNNNNNNGG 2 cut(s) 393, 1058
BsmAI GTCTC 4 cut(s) 242, 1467, 2061, 2159
BsmBI CGTCTC 3 cut(s) 242, 1467, 2061
BsmFI GGGAC 4 cut(s) 143, 492, 1348, 1583
BsmI GAATGC 2 cut(s) 707, 1294
BsnI GGCC 6 cut(s) 197, 363, 1190, 1599, 1710, 2012
Bso31I GGTCTC 1 cut(s) 2159
BsoBI CYCGRG 2 cut(s) 239, 530
Bsp119I TTCGAA 3 cut(s) 301, 797, 1990
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
Bsp19I CCATGG 1 cut(s) 1564
BspANI GGCC 6 cut(s) 197, 363, 1190, 1599, 1710, 2012
BspCNI CTCAG 4 cut(s) 683, 1798, 1870, 2155
BspFNI CGCG 3 cut(s) 851, 1193, 1455
BspLI GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 934, 1571, 1600
BspMAI CTGCAG 3 cut(s) 10, 519, 2158
BspMI ACCTGC 2 cut(s) 508, 2161
BspPI GGATC 3 cut(s) 227, 403, 1949
BspT104I TTCGAA 3 cut(s) 301, 797, 1990
BspTNI GGTCTC 1 cut(s) 2159
BsrDI GCAATG 7 cut(s) 197, 775, 1216, 1299, 1591, 1613, 2120
BsrI ACTGG 3 cut(s) 488, 1101, 1484
BssECI CCNNGG 3 cut(s) 1191, 1564, 1958
BssMI GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
BssT1I CCWWGG 2 cut(s) 1564, 1958
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 4 cut(s) 1461, 1968, 2058, 2072
Bst6I CTCTTC 5 cut(s) 623, 1146, 1383, 1657, 2089
BstAPI GCANNNNNTGC 2 cut(s) 523, 702
BstBI TTCGAA 3 cut(s) 301, 797, 1990
BstC8I GCNNGC 2 cut(s) 1430, 1485
BstDEI CTNAG 5 cut(s) 670, 1785, 1878, 2063, 2163
BstDSI CCRYGG 2 cut(s) 1191, 1564
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 5 cut(s) 339, 1131, 1224, 1413, 1527
BstFNI CGCG 3 cut(s) 851, 1193, 1455
BstHHI GCGC 3 cut(s) 190, 851, 1089
BstKTI GATC 8 cut(s) 222, 411, 893, 958, 1135, 1453, 1647, 1957
BstMAI GTCTC 4 cut(s) 242, 1467, 2061, 2159
BstMBI GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
BstMCI CGRYCG 1 cut(s) 1838
BstNI CCWGG 1 cut(s) 388
BstNSI RCATGY 1 cut(s) 1487
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 14, 386, 558
BstSFI CTRYAG 4 cut(s) 6, 515, 1378, 2154
BstUI CGCG 3 cut(s) 851, 1193, 1455
BstV1I GCAGC 7 cut(s) 20, 1106, 1437, 1949, 2020, 2146, 2168
BstV2I GAAGAC 3 cut(s) 224, 290, 2034
BstX2I RGATCY 1 cut(s) 408
BstXI CCANNNNNNTGG 1 cut(s) 313
BstYI RGATCY 1 cut(s) 408
BsuRI GGCC 6 cut(s) 197, 363, 1190, 1599, 1710, 2012
BtgI CCRYGG 2 cut(s) 1191, 1564
BtgZI GCGATG 1 cut(s) 1743
BtsCI GGATG 5 cut(s) 339, 1131, 1224, 1413, 1527
BtsIMutI CAGTG 2 cut(s) 951, 1912
BveI ACCTGC 2 cut(s) 508, 2161
Cac8I GCNNGC 2 cut(s) 1430, 1485
CaiI CAGNNNCTG 2 cut(s) 523, 2162
CfoI GCGC 3 cut(s) 190, 851, 1089
Cfr13I GGNCC 7 cut(s) 157, 195, 362, 460, 1457, 1570, 1598
Cfr42I CCGCGG 1 cut(s) 1194
CpoI CGGWCCG 1 cut(s) 1457
CseI GACGC 2 cut(s) 1345, 2039
Csp6I GTAC 7 cut(s) 37, 521, 1095, 1231, 1411, 1589, 1944
CspCI CAANNNNNGTGG 2 cut(s) 1653, 1688
CspI CGGWCCG 1 cut(s) 1457
CviQI GTAC 7 cut(s) 37, 521, 1095, 1231, 1411, 1589, 1944
DdeI CTNAG 5 cut(s) 670, 1785, 1878, 2063, 2163
DpnI GATC 8 cut(s) 221, 410, 892, 957, 1134, 1452, 1646, 1956
DpnII GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
DriI GACNNNNNGTC 2 cut(s) 1341, 1576
EaeI YGGCCR 1 cut(s) 1708
Eam1104I CTCTTC 5 cut(s) 623, 1146, 1383, 1657, 2089
Eam1105I GACNNNNNGTC 2 cut(s) 1341, 1576
EarI CTCTTC 5 cut(s) 623, 1146, 1383, 1657, 2089
EciI GGCGGA 2 cut(s) 515, 1022
Eco130I CCWWGG 2 cut(s) 1564, 1958
Eco24I GRGCYC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 2159
Eco32I GATATC 1 cut(s) 2143
Eco47I GGWCC 4 cut(s) 157, 460, 1457, 1570
Eco88I CYCGRG 2 cut(s) 239, 530
Eco91I GGTNACC 1 cut(s) 260
EcoO109I RGGNCCY 2 cut(s) 157, 1570
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 386
EcoRV GATATC 1 cut(s) 2143
EcoT14I CCWWGG 2 cut(s) 1564, 1958
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 2 cut(s) 1564, 1958
Esp3I CGTCTC 3 cut(s) 242, 1467, 2061
FalI AAGNNNNNCTT 2 cut(s) 1412, 1444
FaqI GGGAC 4 cut(s) 143, 492, 1348, 1583
FauI CCCGC 4 cut(s) 470, 542, 1115, 2118
FbaI TGATCA 2 cut(s) 955, 1644
FokI GGATG 5 cut(s) 326, 1138, 1231, 1400, 1534
FriOI GRGCYC 1 cut(s) 21
FspBI CTAG 7 cut(s) 266, 1110, 1136, 1488, 1742, 2022, 2037
FspI TGCGCA 1 cut(s) 189
GlaI GCGC 3 cut(s) 189, 850, 1088
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 6 cut(s) 197, 363, 1190, 1599, 1710, 2012
HapII CCGG 5 cut(s) 15, 198, 333, 402, 560
HgaI GACGC 2 cut(s) 1345, 2039
HhaI GCGC 3 cut(s) 190, 851, 1089
Hin6I GCGC 3 cut(s) 188, 849, 1087
HinP1I GCGC 3 cut(s) 188, 849, 1087
HincII GTYRAC 1 cut(s) 1173
HindII GTYRAC 1 cut(s) 1173
HindIII AAGCTT 1 cut(s) 1695
HinfI GANTC 8 cut(s) 149, 201, 275, 437, 622, 1360, 1746, 2081
HpaII CCGG 5 cut(s) 15, 198, 333, 402, 560
HphI GGTGA 9 cut(s) 20, 121, 418, 726, 732, 738, 1279, 1460, 1710
Hpy166II GTNNAC 2 cut(s) 319, 1173
Hpy188I TCNGA 7 cut(s) 418, 627, 890, 1084, 1624, 1849, 2201
Hpy188III TCNNGA 7 cut(s) 530, 532, 663, 971, 1357, 1466, 1757
Hpy8I GTNNAC 2 cut(s) 319, 1173
Hpy99I CGWCG 1 cut(s) 1231
HpyAV CCTTC 7 cut(s) 77, 884, 898, 1150, 1180, 1791, 1986
HpyCH4III ACNGT 4 cut(s) 1461, 1968, 2058, 2072
HpyCH4IV ACGT 3 cut(s) 354, 981, 1342
HpyF3I CTNAG 5 cut(s) 670, 1785, 1878, 2063, 2163
HpySE526I ACGT 3 cut(s) 354, 981, 1342
HspAI GCGC 3 cut(s) 188, 849, 1087
KflI GGGWCCC 1 cut(s) 157
Ksp22I TGATCA 2 cut(s) 955, 1644
KspI CCGCGG 1 cut(s) 1194
Kzo9I GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
LmnI GCTCC 6 cut(s) 341, 552, 721, 1084, 1642, 1926
Lsp1109I GCAGC 7 cut(s) 20, 1106, 1437, 1949, 2020, 2146, 2168
LweI GCATC 3 cut(s) 175, 535, 1303
MaeI CTAG 7 cut(s) 266, 1110, 1136, 1488, 1742, 2022, 2037
MaeII ACGT 3 cut(s) 354, 981, 1342
MaeIII GTNAC 5 cut(s) 223, 260, 841, 1440, 2058
MalI GATC 8 cut(s) 221, 410, 892, 957, 1134, 1452, 1646, 1956
MboI GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
MfeI CAATTG 1 cut(s) 822
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 1710
MluNI TGGCCA 1 cut(s) 1710
MlyI GAGTC 5 cut(s) 195, 431, 1354, 1755, 2075
MmeI TCCRAC 1 cut(s) 1959
Mox20I TGGCCA 1 cut(s) 1710
MscI TGGCCA 1 cut(s) 1710
MseI TTAA 5 cut(s) 77, 213, 1400, 1476, 1493
MslI CAYNNNNRTG 4 cut(s) 431, 1350, 1723, 1932
Msp20I TGGCCA 1 cut(s) 1710
MspA1I CMGCKG 5 cut(s) 11, 477, 549, 1193, 2159
MspI CCGG 5 cut(s) 15, 198, 333, 402, 560
MspR9I CCNGG 3 cut(s) 16, 388, 560
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 2 cut(s) 707, 1294
MvaI CCWGG 1 cut(s) 388
MvnI CGCG 3 cut(s) 851, 1193, 1455
NciI CCSGG 2 cut(s) 16, 560
NcoI CCATGG 1 cut(s) 1564
NdeII GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
NlaIV GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 934, 1571, 1600
NmuCI GTSAC 1 cut(s) 2058
NsbI TGCGCA 1 cut(s) 189
NspI RCATGY 1 cut(s) 1487
NspV TTCGAA 3 cut(s) 301, 797, 1990
OliI CACNNNNGTG 2 cut(s) 1723, 1932
PaeI GCATGC 1 cut(s) 1487
PaeR7I CTCGAG 1 cut(s) 530
PaqCI CACCTGC 1 cut(s) 2161
PctI GAATGC 2 cut(s) 707, 1294
PfeI GAWTC 3 cut(s) 149, 275, 622
PflMI CCANNNNNTGG 1 cut(s) 1058
PfoI TCCNGGA 2 cut(s) 386, 558
PleI GAGTC 5 cut(s) 195, 431, 1354, 1754, 2075
PpsI GAGTC 5 cut(s) 195, 431, 1354, 1754, 2075
PpuMI RGGWCCY 2 cut(s) 157, 1570
Psp5II RGGWCCY 2 cut(s) 157, 1570
Psp6I CCWGG 1 cut(s) 386
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 934, 1571, 1600
PspPI GGNCC 7 cut(s) 157, 195, 362, 460, 1457, 1570, 1598
PspPPI RGGWCCY 2 cut(s) 157, 1570
PstI CTGCAG 3 cut(s) 10, 519, 2158
PstNI CAGNNNCTG 2 cut(s) 523, 2162
PsuI RGATCY 1 cut(s) 408
PvuII CAGCTG 2 cut(s) 11, 2159
RsaI GTAC 7 cut(s) 38, 522, 1096, 1232, 1412, 1590, 1945
RsaNI GTAC 7 cut(s) 37, 521, 1095, 1231, 1411, 1589, 1944
RseI CAYNNNNRTG 4 cut(s) 431, 1350, 1723, 1932
Rsr2I CGGWCCG 1 cut(s) 1457
RsrII CGGWCCG 1 cut(s) 1457
SacII CCGCGG 1 cut(s) 1194
SaqAI TTAA 5 cut(s) 77, 213, 1400, 1476, 1493
Sau3AI GATC 8 cut(s) 219, 408, 890, 955, 1132, 1450, 1644, 1954
Sau96I GGNCC 7 cut(s) 157, 195, 362, 460, 1457, 1570, 1598
ScaI AGTACT 1 cut(s) 1096
SchI GAGTC 5 cut(s) 195, 431, 1354, 1755, 2075
ScrFI CCNGG 3 cut(s) 16, 388, 560
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 3 cut(s) 175, 535, 1303
SfcI CTRYAG 4 cut(s) 6, 515, 1378, 2154
Sfr274I CTCGAG 1 cut(s) 530
Sfr303I CCGCGG 1 cut(s) 1194
SfuI TTCGAA 3 cut(s) 301, 797, 1990
SgrBI CCGCGG 1 cut(s) 1194
SinI GGWCC 4 cut(s) 157, 460, 1457, 1570
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 4 cut(s) 431, 1350, 1723, 1932
SmlI CTYRAG 3 cut(s) 110, 530, 969
SmoI CTYRAG 3 cut(s) 110, 530, 969
SpeI ACTAGT 1 cut(s) 2021
SphI GCATGC 1 cut(s) 1487
SspMI CTAG 7 cut(s) 266, 1110, 1136, 1488, 1742, 2022, 2037
StyD4I CCNGG 3 cut(s) 14, 386, 558
StyI CCWWGG 2 cut(s) 1564, 1958
TaaI ACNGT 4 cut(s) 1461, 1968, 2058, 2072
TaiI ACGT 3 cut(s) 357, 984, 1345
TaqII GACCGA 1 cut(s) 2183
TatI WGTACW 3 cut(s) 1094, 1410, 1588
TauI GCSGC 2 cut(s) 1193, 1196
TfiI GAWTC 3 cut(s) 149, 275, 622
Tru1I TTAA 5 cut(s) 77, 213, 1400, 1476, 1493
Tru9I TTAA 5 cut(s) 77, 213, 1400, 1476, 1493
TscAI CASTG 2 cut(s) 958, 1912
TseFI GTSAC 1 cut(s) 2058
TseI GCWGC 9 cut(s) 5, 8, 11, 1119, 1425, 1937, 2033, 2156, 2159
Tsp45I GTSAC 1 cut(s) 2058
TspDTI ATGAA 4 cut(s) 300, 581, 1199, 1301
TspGWI ACGGA 3 cut(s) 1190, 1288, 1961
TspRI CASTG 2 cut(s) 958, 1912
Van91I CCANNNNNTGG 1 cut(s) 1058
VpaK11BI GGWCC 4 cut(s) 157, 460, 1457, 1570
XapI RAATTY 6 cut(s) 483, 641, 916, 1541, 1703, 1775
XceI RCATGY 1 cut(s) 1487
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 7 cut(s) 266, 1110, 1136, 1488, 1742, 2022, 2037
ZrmI AGTACT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.