RLG00000020196
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018061
Physical Location & Seq
Reverse (-)
62321676 .. 62324581
2906 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020196

Sequence Viewer

Length: 2205 bp
ATGGCTACAGCTGCCGGGCTCAATCTTCACCGCCCGTACCTCCTCCTCTCCATAATTTTCCTCTCCCTTCCTCCATTAACATATTCGATAACCGAAGCCGAAGCGTTGCTCAAGTTCAAGCAATCCCTCACCAATGCAAACGCTTTGGATTCTTGGGTCCCTAACTCTGCACCATGCACCAAAGATGCGCAATGGGCCGGACTCGTATGCTTTAATGGGATCGTTACGGGTCTTCGTCTCGGGGGAATGGGGTTATCTGGTAACCTAGACTTTGAATCGCTGTATGAAGTCAAGGGTCTTCGAACCATGAGCTTAGTGGACAACAATTTCTCCGGCTCCATCCCTAATTTCAACGTCATTGGCCCCTTGAAAGCTATCTATTTGTCCAGGAATGGGTTTTCCGGCGAGATCCCATCGGACTTTTTCACCAACATGGACTCATTGAAGAAAGTGTGGCTGGACCACAATCAATTAAGCGGGAAAATTCCGGTTTCTCTGGCGGAAAGTCCCAATCTGCAGGTACTGCATCTCGAGAACAATCAGTTCAGCGGGAGCCTCCCGGAAATGAACCAGAAGTCGTTGAACACCCTCAATGTGTCCAATAACAAGCTGGAGGGGGAGATTCCGAAGAGTTTGGCTAAATTTGATGAGGCTGTGTTTTCTGGAAACTCAGGTCTTTGTGGGCAAAAGGTGGGCAAAGAATGCAAAAATGTAGCAGGGGAGCCAACACCATCACCCTCACCAACGAGTTCTAATGAGAGCAATGAGGGTAGTGGCTCGAAATCGAGTTCGAAAAGTAAGGGGGGAAGCTCTACGATTGTTATTGCATTGTCAGTAACTTGCGCGGTGGTGCTTCTCATTCTTATCCTTCTGTTGTTTGTCAGATCAAGGAGGAAGAAGGCAGATGAGAATTTCGATACTCTTGGAACCCAAAATAGTCAAAGCACTGATCGTTCTCCTCCTCAAGAAAGAAACGTAGAAGCAGTTGAGTTGAATGTGGCGGAGATAAATAACAAGGAAAGGGAAGTGGTAGTTGAGCCATCTCCATCAGTTGGTAGTGCTAAAAAGGGTGGCTCGGAGCGCAAGAGTACTTCCAGTCATGCTAGAGGAGGCTGCGGGATGGGCGATCTAGTGATGTTGAGTGAAGAGAAGGGAGTGTTTGGGTTGACGGATTTGATGAAGGCCGCGGCGGAGGTGCTTGGAAATGGGGGGATGGGTTCGTCGTATAAGGCTGTCATGGCGAATGGATTGGCGGTTGTGGTGAAACGGATAAGAGAAATGAATGCAATGGGGAGAGAGGGCTTTGATGCAGAGATGAGGAGGTATGGGACGCTACGTCATTGGAATGTCTTGACTCCTTTGGCTTATCACTACAGAAAAGAAGAGAAGCTGTTAATCTATGAGTACATCCCCAAGAGCAGCTTGCTTTATCTCTTACATGGTGATCGTGGACAGTCTCACGACCAACTTAACTGGCATGCTAGGTTAAAGATTATTCAAGGCACGGCAAGAGGGATGGGTTTTATCCATACTGAATTTTCAGCCTATGATTTGCCCCATGGGGACCTAAAGTCCAGCAATGTACTGATTGGTCCCGACTATGAGCCATTGCTTTCAGACTTTGCATTTGCTCCAATGATCAACTCTTCACATTTGCCACAAGCCTTGTTTGCATACAAAGCCCCAGAAGCTTTAGAATTTGGCCAAGTATCACCCAAGTGCGATGTGTTCTGTCTAGGAGTCCTCATTCTCGAAACACTAACGGGCAAATTCCCTTCTCAGTATCTCAACAATGGCAAAGGTGGGATTGATGTAGTCCAATGGGTGCGGTCGGCTATATCTGAAAGGAGAGAATTGGAGCTATTAGACCCTGAGATTTCAAACGAAAAGGGTTCAATCAGTGAAATGGAGAAGCTCCTCCACATTGGTGCAGCCTGCACGGAGAATGATCCAAGGCAACGATTAGATATGATGGAGGCTATTCGAAGGATAGAGCAGATAAAGGCCATCCAAACTAGTGAAGCTAGAACAATACAAGTGCTACCGTCTCTAAGTGACGGTTGTGCAGACTCTTCTTTAGTAATGCCAAAGACAACTCATGCACCAACATTGCGAGAAGGGGTAGCAGATATCCACGCACCTGCAGCTGCTGAGACCGAGATTAGCAGACTGCCATCAAAGAGCTTCCGAAGTAAATCGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

735

Amino Acids

79.74

Weight (kDa)

6.47

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 70 3.4e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 123 - 182 1.3e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 397 - 658 5.8e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 398 - 661 1.5e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018061)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g35120
prunus_persica Prupe.3G037900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0146351
rosa_laevigata RLG00000020196
rosa_multiflora Rmu_sc0002054.1_g000009
rosa_rugosa Rorug02G0394100
rosa_samantha Rh2AG450600 Rh2BG463000 Rh2CG437400 Rh2DG472000
rosa_wichuraiana Rw2G036780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2149
Acc16I TGCGCA 1 cut(s) 189
Acc36I ACCTGC 2 cut(s) 508, 2149
AccB7I CCANNNNNTGG 1 cut(s) 1052
AccII CGCG 2 cut(s) 845, 1187
AclWI GGATC 3 cut(s) 227, 403, 1943
AcoI YGGCCR 1 cut(s) 1702
AcsI RAATTY 6 cut(s) 483, 641, 910, 1535, 1697, 1769
AfaI GTAC 5 cut(s) 38, 522, 1090, 1406, 1584
AfiI CCNNNNNNNGG 2 cut(s) 393, 1052
AhdI GACNNNNNGTC 2 cut(s) 1335, 1570
AhlI ACTAGT 1 cut(s) 2015
AjnI CCWGG 1 cut(s) 386
AleI CACNNNNGTG 2 cut(s) 1717, 1926
Alw26I GTCTC 4 cut(s) 242, 1461, 2052, 2147
AlwI GGATC 3 cut(s) 227, 403, 1943
AlwNI CAGNNNCTG 2 cut(s) 523, 2150
Ama87I CYCGRG 2 cut(s) 239, 530
AoxI GGCC 5 cut(s) 195, 361, 1182, 1702, 2004
ApeKI GCWGC 6 cut(s) 11, 1113, 1419, 1931, 2144, 2147
ApoI RAATTY 6 cut(s) 483, 641, 910, 1535, 1697, 1769
ArsI GACNNNNNNTTYG 2 cut(s) 1610, 1642
AspLEI GCGC 3 cut(s) 190, 845, 1083
AspS9I GGNCC 6 cut(s) 157, 195, 362, 460, 1564, 1592
AsuC2I CCSGG 2 cut(s) 16, 560
AsuHPI GGTGA 8 cut(s) 20, 121, 418, 726, 732, 1273, 1454, 1704
AsuII TTCGAA 3 cut(s) 301, 791, 1984
AvaI CYCGRG 2 cut(s) 239, 530
AvaII GGWCC 4 cut(s) 157, 460, 1564, 1592
BalI TGGCCA 1 cut(s) 1704
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 2 cut(s) 224, 290
BbvI GCAGC 5 cut(s) 1100, 1431, 1943, 2134, 2156
BceAI ACGGC 1 cut(s) 1521
BciT130I CCWGG 1 cut(s) 388
BclI TGATCA 1 cut(s) 1638
BcnI CCSGG 2 cut(s) 16, 560
BcoDI GTCTC 4 cut(s) 242, 1461, 2052, 2147
BcuI ACTAGT 1 cut(s) 2015
BfaI CTAG 7 cut(s) 266, 1104, 1130, 1482, 1736, 2016, 2025
BfmI CTRYAG 4 cut(s) 6, 515, 1372, 2142
BfuAI ACCTGC 2 cut(s) 508, 2149
BisI GCNGC 8 cut(s) 12, 1114, 1185, 1188, 1420, 1932, 2145, 2148
BlsI GCNGC 8 cut(s) 13, 1115, 1186, 1189, 1421, 1933, 2146, 2149
BmcAI AGTACT 1 cut(s) 1090
Bme1390I CCNGG 3 cut(s) 16, 388, 560
Bme18I GGWCC 4 cut(s) 157, 460, 1564, 1592
BmeRI GACNNNNNGTC 2 cut(s) 1335, 1570
BmeT110I CYCGRG 2 cut(s) 239, 530
BmgT120I GGNCC 6 cut(s) 157, 195, 362, 460, 1564, 1592
BmiI GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 928, 1565, 1594
BmrFI CCNGG 3 cut(s) 16, 388, 560
BmsI GCATC 3 cut(s) 175, 535, 1297
BpiI GAAGAC 2 cut(s) 224, 290
BpmI CTGGAG 1 cut(s) 632
Bpu14I TTCGAA 3 cut(s) 301, 791, 1984
BpuEI CTTGAG 2 cut(s) 95, 948
BpuMI CCSGG 2 cut(s) 16, 560
BsaI GGTCTC 1 cut(s) 2147
BsaJI CCNNGG 3 cut(s) 1185, 1558, 1952
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 1052
Bse1I ACTGG 2 cut(s) 1095, 1478
Bse3DI GCAATG 6 cut(s) 197, 769, 1293, 1585, 1607, 2108
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 1185, 1558, 1952
BseGI GGATG 6 cut(s) 339, 1125, 1218, 1407, 1521, 2007
BseLI CCNNNNNNNGG 2 cut(s) 393, 1052
BseMI GCAATG 6 cut(s) 197, 769, 1293, 1585, 1607, 2108
BseMII CTCAG 4 cut(s) 684, 1793, 1863, 2142
BseNI ACTGG 2 cut(s) 1095, 1478
BseRI GAGGAG 7 cut(s) 32, 35, 948, 951, 1122, 1333, 1907
BseXI GCAGC 5 cut(s) 1100, 1431, 1943, 2134, 2156
BsgI GTGCAG 4 cut(s) 153, 1921, 1950, 2085
Bsh1236I CGCG 2 cut(s) 845, 1187
Bsh1285I CGRYCG 1 cut(s) 1832
BshFI GGCC 5 cut(s) 197, 363, 1184, 1704, 2006
BsiEI CGRYCG 1 cut(s) 1832
BsiHKCI CYCGRG 2 cut(s) 239, 530
BsiSI CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
BslFI GGGAC 5 cut(s) 143, 492, 1342, 1577, 1578
BslI CCNNNNNNNGG 2 cut(s) 393, 1052
BsmAI GTCTC 4 cut(s) 242, 1461, 2052, 2147
BsmBI CGTCTC 2 cut(s) 242, 2052
BsmFI GGGAC 5 cut(s) 143, 492, 1342, 1577, 1578
BsmI GAATGC 2 cut(s) 707, 1288
BsnI GGCC 5 cut(s) 197, 363, 1184, 1704, 2006
Bso31I GGTCTC 1 cut(s) 2147
BsoBI CYCGRG 2 cut(s) 239, 530
Bsp119I TTCGAA 3 cut(s) 301, 791, 1984
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
Bsp19I CCATGG 1 cut(s) 1558
BspANI GGCC 5 cut(s) 197, 363, 1184, 1704, 2006
BspCNI CTCAG 4 cut(s) 683, 1792, 1864, 2143
BspFNI CGCG 2 cut(s) 845, 1187
BspLI GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 928, 1565, 1594
BspMAI CTGCAG 2 cut(s) 519, 2146
BspMI ACCTGC 2 cut(s) 508, 2149
BspPI GGATC 3 cut(s) 227, 403, 1943
BspT104I TTCGAA 3 cut(s) 301, 791, 1984
BspTNI GGTCTC 1 cut(s) 2147
BsrDI GCAATG 6 cut(s) 197, 769, 1293, 1585, 1607, 2108
BsrI ACTGG 2 cut(s) 1095, 1478
BssECI CCNNGG 3 cut(s) 1185, 1558, 1952
BssMI GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
BssT1I CCWWGG 2 cut(s) 1558, 1952
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 3 cut(s) 1455, 2046, 2060
Bst6I CTCTTC 5 cut(s) 623, 1140, 1377, 1651, 2077
BstAPI GCANNNNNTGC 2 cut(s) 523, 702
BstBI TTCGAA 3 cut(s) 301, 791, 1984
BstC8I GCNNGC 3 cut(s) 1424, 1479, 1936
BstDEI CTNAG 6 cut(s) 313, 670, 1779, 1872, 2051, 2151
BstDSI CCRYGG 2 cut(s) 1185, 1558
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 6 cut(s) 339, 1125, 1218, 1407, 1521, 2007
BstFNI CGCG 2 cut(s) 845, 1187
BstHHI GCGC 3 cut(s) 190, 845, 1083
BstKTI GATC 8 cut(s) 222, 411, 887, 952, 1129, 1447, 1641, 1951
BstMAI GTCTC 4 cut(s) 242, 1461, 2052, 2147
BstMBI GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
BstMCI CGRYCG 1 cut(s) 1832
BstNI CCWGG 1 cut(s) 388
BstNSI RCATGY 1 cut(s) 1481
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 14, 386, 558
BstSFI CTRYAG 4 cut(s) 6, 515, 1372, 2142
BstUI CGCG 2 cut(s) 845, 1187
BstV1I GCAGC 5 cut(s) 1100, 1431, 1943, 2134, 2156
BstV2I GAAGAC 2 cut(s) 224, 290
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
BsuRI GGCC 5 cut(s) 197, 363, 1184, 1704, 2006
BtgI CCRYGG 2 cut(s) 1185, 1558
BtgZI GCGATG 1 cut(s) 1737
BtsCI GGATG 6 cut(s) 339, 1125, 1218, 1407, 1521, 2007
BtsIMutI CAGTG 2 cut(s) 945, 1906
BveI ACCTGC 2 cut(s) 508, 2149
Cac8I GCNNGC 3 cut(s) 1424, 1479, 1936
CaiI CAGNNNCTG 2 cut(s) 523, 2150
CfoI GCGC 3 cut(s) 190, 845, 1083
Cfr13I GGNCC 6 cut(s) 157, 195, 362, 460, 1564, 1592
Cfr42I CCGCGG 1 cut(s) 1188
CseI GACGC 1 cut(s) 1339
Csp6I GTAC 5 cut(s) 37, 521, 1089, 1405, 1583
CspCI CAANNNNNGTGG 2 cut(s) 1647, 1682
CviAII CATG 9 cut(s) 174, 307, 433, 1100, 1237, 1439, 1478, 1559, 2099
CviQI GTAC 5 cut(s) 37, 521, 1089, 1405, 1583
DdeI CTNAG 6 cut(s) 313, 670, 1779, 1872, 2051, 2151
DpnI GATC 8 cut(s) 221, 410, 886, 951, 1128, 1446, 1640, 1950
DpnII GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
DriI GACNNNNNGTC 2 cut(s) 1335, 1570
EaeI YGGCCR 1 cut(s) 1702
Eam1104I CTCTTC 5 cut(s) 623, 1140, 1377, 1651, 2077
Eam1105I GACNNNNNGTC 2 cut(s) 1335, 1570
EarI CTCTTC 5 cut(s) 623, 1140, 1377, 1651, 2077
EciI GGCGGA 3 cut(s) 515, 1016, 1205
Eco130I CCWWGG 2 cut(s) 1558, 1952
Eco24I GRGCYC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 2147
Eco32I GATATC 1 cut(s) 2131
Eco47I GGWCC 4 cut(s) 157, 460, 1564, 1592
Eco88I CYCGRG 2 cut(s) 239, 530
Eco91I GGTNACC 1 cut(s) 260
EcoO109I RGGNCCY 2 cut(s) 157, 1564
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 386
EcoRV GATATC 1 cut(s) 2131
EcoT14I CCWWGG 2 cut(s) 1558, 1952
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 2 cut(s) 1558, 1952
Esp3I CGTCTC 2 cut(s) 242, 2052
FaeI CATG 9 cut(s) 177, 310, 436, 1103, 1240, 1442, 1481, 1562, 2102
FalI AAGNNNNNCTT 2 cut(s) 1406, 1438
FaqI GGGAC 5 cut(s) 143, 492, 1342, 1577, 1578
FatI CATG 9 cut(s) 173, 306, 432, 1099, 1236, 1438, 1477, 1558, 2098
FauI CCCGC 3 cut(s) 470, 542, 1109
FbaI TGATCA 1 cut(s) 1638
Fnu4HI GCNGC 8 cut(s) 12, 1114, 1185, 1188, 1420, 1932, 2145, 2148
FokI GGATG 6 cut(s) 326, 1132, 1225, 1394, 1528, 1994
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 8 cut(s) 12, 1114, 1185, 1188, 1420, 1932, 2145, 2148
FspBI CTAG 7 cut(s) 266, 1104, 1130, 1482, 1736, 2016, 2025
FspI TGCGCA 1 cut(s) 189
GlaI GCGC 3 cut(s) 189, 844, 1082
GluI GCNGC 8 cut(s) 12, 1114, 1185, 1188, 1420, 1932, 2145, 2148
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 5 cut(s) 197, 363, 1184, 1704, 2006
HapII CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
HgaI GACGC 1 cut(s) 1339
HhaI GCGC 3 cut(s) 190, 845, 1083
Hin1II CATG 9 cut(s) 177, 310, 436, 1103, 1240, 1442, 1481, 1562, 2102
Hin6I GCGC 3 cut(s) 188, 843, 1081
HinP1I GCGC 3 cut(s) 188, 843, 1081
HincII GTYRAC 1 cut(s) 1167
HindII GTYRAC 1 cut(s) 1167
HindIII AAGCTT 1 cut(s) 1689
HinfI GANTC 8 cut(s) 149, 201, 275, 437, 622, 1354, 1740, 2069
HpaII CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
HphI GGTGA 8 cut(s) 20, 121, 418, 726, 732, 1273, 1454, 1704
Hpy166II GTNNAC 3 cut(s) 319, 1167, 1451
Hpy188I TCNGA 7 cut(s) 418, 627, 884, 1078, 1618, 1843, 2189
Hpy188III TCNNGA 8 cut(s) 530, 532, 663, 965, 1351, 1460, 1595, 1751
Hpy8I GTNNAC 3 cut(s) 319, 1167, 1451
Hpy99I CGWCG 1 cut(s) 1225
HpyAV CCTTC 8 cut(s) 77, 878, 892, 1144, 1174, 1785, 1980, 2111
HpyCH4III ACNGT 3 cut(s) 1455, 2046, 2060
HpyCH4IV ACGT 3 cut(s) 354, 975, 1336
HpyF3I CTNAG 6 cut(s) 313, 670, 1779, 1872, 2051, 2151
HpySE526I ACGT 3 cut(s) 354, 975, 1336
Hsp92II CATG 9 cut(s) 177, 310, 436, 1103, 1240, 1442, 1481, 1562, 2102
HspAI GCGC 3 cut(s) 188, 843, 1081
KflI GGGWCCC 1 cut(s) 157
Ksp22I TGATCA 1 cut(s) 1638
KspI CCGCGG 1 cut(s) 1188
Kzo9I GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
LmnI GCTCC 7 cut(s) 341, 552, 721, 1078, 1636, 1858, 1920
Lsp1109I GCAGC 5 cut(s) 1100, 1431, 1943, 2134, 2156
LweI GCATC 3 cut(s) 175, 535, 1297
MaeI CTAG 7 cut(s) 266, 1104, 1130, 1482, 1736, 2016, 2025
MaeII ACGT 3 cut(s) 354, 975, 1336
MaeIII GTNAC 4 cut(s) 223, 260, 835, 2054
MalI GATC 8 cut(s) 221, 410, 886, 951, 1128, 1446, 1640, 1950
MboI GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 1704
MluNI TGGCCA 1 cut(s) 1704
MlyI GAGTC 5 cut(s) 195, 431, 1348, 1749, 2063
Mox20I TGGCCA 1 cut(s) 1704
MscI TGGCCA 1 cut(s) 1704
MseI TTAA 6 cut(s) 77, 213, 473, 1394, 1470, 1487
MslI CAYNNNNRTG 4 cut(s) 431, 1344, 1717, 1926
Msp20I TGGCCA 1 cut(s) 1704
MspA1I CMGCKG 4 cut(s) 11, 549, 1187, 2147
MspI CCGG 6 cut(s) 15, 198, 333, 402, 488, 560
MspR9I CCNGG 3 cut(s) 16, 388, 560
Mva1269I GAATGC 2 cut(s) 707, 1288
MvaI CCWGG 1 cut(s) 388
MvnI CGCG 2 cut(s) 845, 1187
NciI CCSGG 2 cut(s) 16, 560
NcoI CCATGG 1 cut(s) 1558
NdeII GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
NlaIII CATG 9 cut(s) 177, 310, 436, 1103, 1240, 1442, 1481, 1562, 2102
NlaIV GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 928, 1565, 1594
NmuCI GTSAC 1 cut(s) 2054
NsbI TGCGCA 1 cut(s) 189
NspI RCATGY 1 cut(s) 1481
NspV TTCGAA 3 cut(s) 301, 791, 1984
OliI CACNNNNGTG 2 cut(s) 1717, 1926
PaeI GCATGC 1 cut(s) 1481
PaeR7I CTCGAG 1 cut(s) 530
PaqCI CACCTGC 1 cut(s) 2149
PctI GAATGC 2 cut(s) 707, 1288
PfeI GAWTC 3 cut(s) 149, 275, 622
PflMI CCANNNNNTGG 1 cut(s) 1052
PfoI TCCNGGA 2 cut(s) 386, 558
PkrI GCNGC 8 cut(s) 13, 1115, 1186, 1189, 1421, 1933, 2146, 2149
PleI GAGTC 5 cut(s) 195, 431, 1348, 1748, 2063
PpsI GAGTC 5 cut(s) 195, 431, 1348, 1748, 2063
PpuMI RGGWCCY 2 cut(s) 157, 1564
Psp5II RGGWCCY 2 cut(s) 157, 1564
Psp6I CCWGG 1 cut(s) 386
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 9 cut(s) 158, 159, 337, 364, 554, 723, 928, 1565, 1594
PspPI GGNCC 6 cut(s) 157, 195, 362, 460, 1564, 1592
PspPPI RGGWCCY 2 cut(s) 157, 1564
PstI CTGCAG 2 cut(s) 519, 2146
PstNI CAGNNNCTG 2 cut(s) 523, 2150
PsuI RGATCY 1 cut(s) 408
PvuII CAGCTG 2 cut(s) 11, 2147
RsaI GTAC 5 cut(s) 38, 522, 1090, 1406, 1584
RsaNI GTAC 5 cut(s) 37, 521, 1089, 1405, 1583
RseI CAYNNNNRTG 4 cut(s) 431, 1344, 1717, 1926
SacII CCGCGG 1 cut(s) 1188
SaqAI TTAA 6 cut(s) 77, 213, 473, 1394, 1470, 1487
SatI GCNGC 8 cut(s) 12, 1114, 1185, 1188, 1420, 1932, 2145, 2148
Sau3AI GATC 8 cut(s) 219, 408, 884, 949, 1126, 1444, 1638, 1948
Sau96I GGNCC 6 cut(s) 157, 195, 362, 460, 1564, 1592
ScaI AGTACT 1 cut(s) 1090
SchI GAGTC 5 cut(s) 195, 431, 1348, 1749, 2063
ScrFI CCNGG 3 cut(s) 16, 388, 560
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 3 cut(s) 175, 535, 1297
SfcI CTRYAG 4 cut(s) 6, 515, 1372, 2142
Sfr274I CTCGAG 1 cut(s) 530
Sfr303I CCGCGG 1 cut(s) 1188
SfuI TTCGAA 3 cut(s) 301, 791, 1984
SgrBI CCGCGG 1 cut(s) 1188
SinI GGWCC 4 cut(s) 157, 460, 1564, 1592
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 4 cut(s) 431, 1344, 1717, 1926
SmlI CTYRAG 3 cut(s) 110, 530, 963
SmoI CTYRAG 3 cut(s) 110, 530, 963
SpeI ACTAGT 1 cut(s) 2015
SphI GCATGC 1 cut(s) 1481
SspMI CTAG 7 cut(s) 266, 1104, 1130, 1482, 1736, 2016, 2025
StyD4I CCNGG 3 cut(s) 14, 386, 558
StyI CCWWGG 2 cut(s) 1558, 1952
TaaI ACNGT 3 cut(s) 1455, 2046, 2060
TaiI ACGT 3 cut(s) 357, 978, 1339
TaqI TCGA 9 cut(s) 86, 301, 531, 779, 785, 791, 915, 1752, 1984
TaqII GACCGA 1 cut(s) 2171
TatI WGTACW 3 cut(s) 1088, 1404, 1582
TauI GCSGC 2 cut(s) 1187, 1190
TfiI GAWTC 3 cut(s) 149, 275, 622
Tru1I TTAA 6 cut(s) 77, 213, 473, 1394, 1470, 1487
Tru9I TTAA 6 cut(s) 77, 213, 473, 1394, 1470, 1487
TscAI CASTG 2 cut(s) 952, 1906
TseFI GTSAC 1 cut(s) 2054
TseI GCWGC 6 cut(s) 11, 1113, 1419, 1931, 2144, 2147
Tsp45I GTSAC 1 cut(s) 2054
TspDTI ATGAA 4 cut(s) 300, 581, 1193, 1295
TspGWI ACGGA 3 cut(s) 1184, 1282, 1955
TspRI CASTG 2 cut(s) 952, 1906
Van91I CCANNNNNTGG 1 cut(s) 1052
VpaK11BI GGWCC 4 cut(s) 157, 460, 1564, 1592
XapI RAATTY 6 cut(s) 483, 641, 910, 1535, 1697, 1769
XceI RCATGY 1 cut(s) 1481
XcmI CCANNNNNNNNNTGG 2 cut(s) 313, 607
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 7 cut(s) 266, 1104, 1130, 1482, 1736, 2016, 2025
ZrmI AGTACT 1 cut(s) 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.