FvH4_6g37470

(R)-mandelonitrile lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014093
Physical Location & Seq
Reverse (-)
29508542 .. 29511464
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37470.t2

Sequence Viewer

Length: 1731 bp
ATGTTCTTGAAGAAAAACAGAGAGGGCTCAACAATGGCGGCGAGACCCATTTCGCTTGGTCCACTTCTTCTCCAGGCGCTTGCTTTCTTGGTGCTGATCAATGCCGTCGCCGCTTCTTCACGGCCGCCGTCTCGGAACCCACACCGCACTCGTCAAGAGCCAAGTTACATGAAGTTTGTTTCCAACGCCACTGACTTCCCTTTGGAGGACTACTATGACTACATTATCGTGGGAGGTGGCACTGCTGGGTGCCCTTTGGCTGCGACGCTGTCATTCAGGTTTCGCGTCCTCCTGCTCGAGAGAGGTGGTGTTGCTTATGGCAATCGGAACTTGATGACCCAGGAAGGTTTCTTGGCCTCACTCCTGGATGTCAACGCCTTTGACTCTGCGACAGAAGCCTTCACATCTGAAGATGGCGTTCCAAACGTCCGAGGACGCATTCTTGGAGGCAGCAGTGCCATAAATGCTGGTTTCTACAGCCGTGCAGATCAGGAGTTCTACAAAAGATCCACTTTCAATTGGGATCTTAAACTGGTGAATGAGTCCTATGATTGGGTCGAAAGAGCAATTGTCTTCAGGCCTGAGCTTAGGAATTGGCAGTCAGCATTTCGAGATGGCTTATTGGAAGCTGGTGTTGATCCCTATAATGGGTATAGTTTTGATCATGTGGTGGGGACCAAGACTGGTGGTTCAACATTTGATACTTTGGGAAGGAGACACAGTGCTGCCGACCTCCTTAAATATGCAAAGCCATTGAATATTCGAGTTGCAGTGCATGCCACTGTGGAAAGAGTTCTATTGGCTTCATCTACTTCATCTCCGGTGTCGAGGCAATCTGCCGTTGGGGTTGTTTTTCGTGACAGAAGTGGGAGGTATCACCATGCTATGCTAAGAAAACACGGAGAGGTGCTTCTCTCAGCAGGTGCAATTGGAACTCCACAGCTACTTCTACTGAGTGGTATTGGCCCAAGGCCTTATCTTGCATCTTGGGGGATTCCAGTGGCTTATCATCTTCCTTATGTTGGGCAGTTTATGTTTGATAATCCCAGAAATGGCATCTCAATAGTGCCACCAATTCCACTGGAACACTCACTCATACAAGTGGTTGGCATTACTGAATCAGGGGCCTACATTGAATCAGCCTCCACTGTAATCCAATTTGCATCCCCAGCCCACTCTGGATTCCTTAGGACACCAGCTGCGCCTCTATATCTTACTGTGGCCACCCTCATGGAGAAGATTGTTGGGCCAGTCTCTGCTGGTTCTTTAAGGTTGGCTTCAACTGATGTCAAGGTGAATCCTATTGTTCGATTCAATTACTTCAACAGCCCAGTGGATGTGCAAAGGTGTGTGAATGGAACGCGAAAGATTGGTGAGATTCTCAGGACCAGGTCTATGGATGATTTCAAGTTCCAGGGATGGTTTGGGAACAAGGATTTTAGGTTTATAGGGCCTGCATTGCCACTTGATCAGTTTGACTTTGACGAGATGGCAGATTTCTGCCGCCGCACTGTAAGCACCATATGGCACTACCATGGGGGCTGCATTGCTGGGAAGGTGGTTGATCGTGATTTCCGGGTCATTGGCATAGATGCACTCCGTGTTGTTGATGGGTCTACTTTTGGTATATCACCAGGGACAAATCCACAGGCTACCCTTCTGATGCTCGGAAGATATGTTGGGCAGAAGATAATGAATGACAGAAAGGGAGGATATAAAATGGTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

577

Amino Acids

63.37

Weight (kDa)

9.35

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 16 - 288 3.7e-19 GMC oxidoreductase
GMC_oxred_C PF05199 361 - 501 4e-27 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73050
fragaria_vesca FvH4_6g37470 FvH4_6g37470
malus_domestica MD09G1157800.v1.1 MD17G1143800.v1.1
prunus_persica Prupe.3G168200_v2.0.a1
pyrus_communis pycom17g13690
rosa_chinensis RchiOBHm_Chr2g0150311
rosa_laevigata RLG00000020486
rosa_multiflora Rmu_sc0002179.1_g000001 Rmu_sc0025680.1_g000002
rosa_roxburghii Rroxscaffold_2G00097300
rosa_rugosa Rorug02G0420400
rosa_samantha Rh2AG479400 Rh2BG491700 Rh2DG500500
rosa_wichuraiana Rw2G039130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 911
Acc36I ACCTGC 1 cut(s) 911
AccB1I GGYRCC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 1616
AccII CGCG 2 cut(s) 285, 1363
AciI CCGC 6 cut(s) 38, 111, 125, 145, 1504, 1507
AclWI GGATC 3 cut(s) 501, 531, 632
AcoI YGGCCR 2 cut(s) 122, 1221
AcuI CTGAAG 2 cut(s) 429, 559
AdeI CACNNNGTG 1 cut(s) 1601
AfiI CCNNNNNNNGG 6 cut(s) 205, 552, 647, 648, 1022, 1052
AgsI TTSAA 9 cut(s) 10, 517, 693, 757, 1136, 1281, 1315, 1324, 1408
AjnI CCWGG 6 cut(s) 72, 339, 363, 1388, 1413, 1633
AluBI AGCT 4 cut(s) 586, 629, 943, 1199
AluI AGCT 4 cut(s) 586, 629, 943, 1199
Alw26I GTCTC 4 cut(s) 37, 135, 709, 1258
AlwI GGATC 3 cut(s) 501, 531, 632
AlwNI CAGNNNCTG 1 cut(s) 1256
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 9 cut(s) 122, 354, 578, 964, 971, 1125, 1221, 1247, 1451
ApeKI GCWGC 5 cut(s) 260, 450, 725, 1199, 1542
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 2 cut(s) 79, 1204
AspS9I GGNCC 7 cut(s) 59, 675, 965, 1125, 1247, 1386, 1451
AsuC2I CCSGG 1 cut(s) 1577
AsuHPI GGTGA 5 cut(s) 547, 869, 1306, 1385, 1623
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 3 cut(s) 59, 675, 1386
AxyI CCTNAGG 1 cut(s) 1187
BaeGI GKGCMC 1 cut(s) 254
BalI TGGCCA 1 cut(s) 1223
BanI GGYRCC 1 cut(s) 249
BanII GRGCYC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 565
BbvI GCAGC 5 cut(s) 247, 462, 712, 1186, 1529
BccI CCATC 5 cut(s) 407, 608, 1413, 1483, 1604
BceAI ACGGC 5 cut(s) 89, 112, 137, 465, 824
BciT130I CCWGG 6 cut(s) 74, 341, 365, 1390, 1415, 1635
BclI TGATCA 3 cut(s) 96, 661, 1468
BcnI CCSGG 1 cut(s) 1577
BcoDI GTCTC 4 cut(s) 37, 135, 709, 1258
BfmI CTRYAG 1 cut(s) 475
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 911
Bme1390I CCNGG 7 cut(s) 74, 341, 365, 1390, 1415, 1577, 1635
Bme18I GGWCC 3 cut(s) 59, 675, 1386
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 7 cut(s) 59, 675, 965, 1125, 1247, 1386, 1451
BmiI GGNNCC 4 cut(s) 137, 251, 676, 1126
BmrFI CCNGG 7 cut(s) 74, 341, 365, 1390, 1415, 1577, 1635
BmrI ACTGGG 1 cut(s) 1325
BmsI GCATC 5 cut(s) 992, 1065, 1172, 1582, 1653
BmuI ACTGGG 1 cut(s) 1325
BpiI GAAGAC 1 cut(s) 565
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 2 cut(s) 582, 587
BpuMI CCSGG 1 cut(s) 1577
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 6 cut(s) 339, 430, 968, 1414, 1534, 1634
BsaWI WCCGGW 1 cut(s) 820
BsaXI ACNNNNNCTCC 6 cut(s) 54, 84, 706, 736, 802, 832
Bsc4I CCNNNNNNNGG 6 cut(s) 205, 552, 647, 648, 1022, 1052
Bse1I ACTGG 6 cut(s) 537, 688, 998, 1086, 1250, 1331
Bse21I CCTNAGG 1 cut(s) 1187
Bse3DI GCAATG 2 cut(s) 1457, 1545
BseBI CCWGG 6 cut(s) 74, 341, 365, 1390, 1415, 1635
BseDI CCNNGG 6 cut(s) 339, 430, 968, 1414, 1534, 1634
BseGI GGATG 5 cut(s) 373, 1163, 1342, 1405, 1424
BseLI CCNNNNNNNGG 6 cut(s) 205, 552, 647, 648, 1022, 1052
BseMI GCAATG 2 cut(s) 1457, 1545
BseMII CTCAG 4 cut(s) 573, 930, 944, 1396
BseNI ACTGG 6 cut(s) 537, 688, 998, 1086, 1250, 1331
BseSI GKGCMC 1 cut(s) 254
BseX3I CGGCCG 1 cut(s) 122
BseXI GCAGC 5 cut(s) 247, 462, 712, 1186, 1529
BseYI CCCAGC 3 cut(s) 245, 1168, 1550
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 2 cut(s) 285, 1363
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 9 cut(s) 124, 356, 580, 966, 973, 1127, 1223, 1249, 1453
BshNI GGYRCC 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 2 cut(s) 821, 1576
BslFI GGGAC 2 cut(s) 688, 1651
BslI CCNNNNNNNGG 6 cut(s) 205, 552, 647, 648, 1022, 1052
BsmAI GTCTC 4 cut(s) 37, 135, 709, 1258
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 688, 1651
BsmI GAATGC 1 cut(s) 438
BsnI GGCC 9 cut(s) 124, 356, 580, 966, 973, 1127, 1223, 1249, 1453
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 29, 254
Bsp143I GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
Bsp19I CCATGG 1 cut(s) 1534
BspACI CCGC 6 cut(s) 38, 111, 125, 145, 1504, 1507
BspANI GGCC 9 cut(s) 124, 356, 580, 966, 973, 1127, 1223, 1249, 1453
BspCNI CTCAG 4 cut(s) 574, 929, 945, 1395
BspFNI CGCG 2 cut(s) 285, 1363
BspLI GGNNCC 4 cut(s) 137, 251, 676, 1126
BspMI ACCTGC 1 cut(s) 911
BspPI GGATC 3 cut(s) 501, 531, 632
BspT107I GGYRCC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 37
BsrDI GCAATG 2 cut(s) 1457, 1545
BsrI ACTGG 6 cut(s) 537, 688, 998, 1086, 1250, 1331
BssECI CCNNGG 6 cut(s) 339, 430, 968, 1414, 1534, 1634
BssMI GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
BssT1I CCWWGG 2 cut(s) 968, 1534
Bst2UI CCWGG 6 cut(s) 74, 341, 365, 1390, 1415, 1635
Bst4CI ACNGT 5 cut(s) 722, 784, 1150, 1219, 1513
BstAPI GCANNNNNTGC 1 cut(s) 776
BstC8I GCNNGC 3 cut(s) 81, 777, 1455
BstDEI CTNAG 7 cut(s) 582, 587, 890, 916, 953, 1187, 1382
BstDSI CCRYGG 1 cut(s) 1534
BstF5I GGATG 5 cut(s) 373, 1163, 1342, 1405, 1424
BstFNI CGCG 2 cut(s) 285, 1363
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 2 cut(s) 79, 1204
BstKTI GATC 8 cut(s) 99, 490, 509, 526, 640, 664, 1471, 1567
BstMAI GTCTC 4 cut(s) 37, 135, 709, 1258
BstMBI GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 7 cut(s) 110, 395, 464, 776, 1169, 1459, 1515
BstNI CCWGG 6 cut(s) 74, 341, 365, 1390, 1415, 1635
BstNSI RCATGY 1 cut(s) 779
BstSCI CCNGG 7 cut(s) 72, 339, 363, 1388, 1413, 1575, 1633
BstSFI CTRYAG 1 cut(s) 475
BstSLI GKGCMC 1 cut(s) 254
BstUI CGCG 2 cut(s) 285, 1363
BstV1I GCAGC 5 cut(s) 247, 462, 712, 1186, 1529
BstV2I GAAGAC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 506, 523
BstXI CCANNNNNNTGG 2 cut(s) 1231, 1396
BstYI RGATCY 2 cut(s) 506, 523
BstZI CGGCCG 1 cut(s) 122
Bsu36I CCTNAGG 1 cut(s) 1187
BsuRI GGCC 9 cut(s) 124, 356, 580, 966, 973, 1127, 1223, 1249, 1453
BtgI CCRYGG 1 cut(s) 1534
BtsCI GGATG 5 cut(s) 373, 1163, 1342, 1405, 1424
BtsI GCAGTG 3 cut(s) 240, 460, 777
BveI ACCTGC 1 cut(s) 911
Cac8I GCNNGC 3 cut(s) 81, 777, 1455
CaiI CAGNNNCTG 1 cut(s) 1256
CfoI GCGC 2 cut(s) 79, 1204
Cfr13I GGNCC 7 cut(s) 59, 675, 965, 1125, 1247, 1386, 1451
CseI GACGC 3 cut(s) 274, 274, 444
CsiI ACCWGGT 1 cut(s) 1388
CspCI CAANNNNNGTGG 2 cut(s) 667, 702
CviAII CATG 6 cut(s) 169, 665, 776, 881, 1231, 1535
DdeI CTNAG 7 cut(s) 582, 587, 890, 916, 953, 1187, 1382
DpnI GATC 8 cut(s) 98, 489, 508, 525, 639, 663, 1470, 1566
DpnII GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
DraIII CACNNNGTG 1 cut(s) 1601
EaeI YGGCCR 2 cut(s) 122, 1221
EagI CGGCCG 1 cut(s) 122
EclXI CGGCCG 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 968, 1534
Eco147I AGGCCT 2 cut(s) 580, 973
Eco24I GRGCYC 1 cut(s) 29
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 3 cut(s) 59, 675, 1386
Eco52I CGGCCG 1 cut(s) 122
Eco57I CTGAAG 2 cut(s) 429, 559
Eco81I CCTNAGG 1 cut(s) 1187
Eco88I CYCGRG 1 cut(s) 296
EcoO109I RGGNCCY 2 cut(s) 1125, 1451
EcoRII CCWGG 6 cut(s) 72, 339, 363, 1388, 1413, 1633
EcoT14I CCWWGG 2 cut(s) 968, 1534
EcoT38I GRGCYC 1 cut(s) 29
ErhI CCWWGG 2 cut(s) 968, 1534
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 6 cut(s) 172, 668, 779, 884, 1234, 1538
FalI AAGNNNNNCTT 4 cut(s) 496, 528, 1261, 1293
FaqI GGGAC 2 cut(s) 688, 1651
FatI CATG 6 cut(s) 168, 664, 775, 880, 1230, 1534
FauNDI CATATG 1 cut(s) 1523
FbaI TGATCA 3 cut(s) 96, 661, 1468
FblI GTMKAC 1 cut(s) 1616
FokI GGATG 5 cut(s) 380, 1150, 1349, 1412, 1431
FriOI GRGCYC 1 cut(s) 29
GlaI GCGC 2 cut(s) 78, 1203
GsaI CCCAGC 3 cut(s) 249, 1172, 1554
GsuI CTGGAG 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 9 cut(s) 124, 356, 580, 966, 973, 1127, 1223, 1249, 1453
HapII CCGG 2 cut(s) 821, 1576
HgaI GACGC 3 cut(s) 274, 274, 444
HhaI GCGC 2 cut(s) 79, 1204
Hin1II CATG 6 cut(s) 172, 668, 779, 884, 1234, 1538
Hin6I GCGC 2 cut(s) 77, 1202
HinP1I GCGC 2 cut(s) 77, 1202
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 9 cut(s) 383, 542, 994, 1118, 1136, 1182, 1297, 1311, 1378
HpaII CCGG 2 cut(s) 821, 1576
HphI GGTGA 5 cut(s) 547, 869, 1306, 1385, 1623
Hpy166II GTNNAC 3 cut(s) 62, 373, 1617
Hpy188I TCNGA 6 cut(s) 135, 327, 409, 431, 1662, 1670
Hpy188III TCNNGA 9 cut(s) 7, 155, 298, 491, 611, 857, 1179, 1384, 1568
Hpy8I GTNNAC 3 cut(s) 62, 373, 1617
Hpy99I CGWCG 2 cut(s) 110, 268
HpyAV CCTTC 5 cut(s) 338, 409, 705, 1549, 1667
HpyCH4III ACNGT 5 cut(s) 722, 784, 1150, 1219, 1513
HpyCH4IV ACGT 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 395, 464, 776, 1169, 1459, 1515
HpyF3I CTNAG 7 cut(s) 582, 587, 890, 916, 953, 1187, 1382
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 6 cut(s) 172, 668, 779, 884, 1234, 1538
HspAI GCGC 2 cut(s) 77, 1202
Ksp22I TGATCA 3 cut(s) 96, 661, 1468
Kzo9I GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
Lsp1109I GCAGC 5 cut(s) 247, 462, 712, 1186, 1529
LweI GCATC 5 cut(s) 992, 1065, 1172, 1582, 1653
MabI ACCWGGT 1 cut(s) 1388
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 164, 857
MalI GATC 8 cut(s) 98, 489, 508, 525, 639, 663, 1470, 1566
MboI GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
MboII GAAGA 9 cut(s) 22, 59, 108, 422, 565, 1004, 1249, 1683, 1699
MfeI CAATTG 3 cut(s) 517, 567, 927
MflI RGATCY 2 cut(s) 506, 523
MhlI GDGCHC 2 cut(s) 29, 254
MlsI TGGCCA 1 cut(s) 1223
MluCI AATT 7 cut(s) 517, 567, 592, 927, 1074, 1157, 1315
MluNI TGGCCA 1 cut(s) 1223
MlyI GAGTC 2 cut(s) 377, 551
MmeI TCCRAC 1 cut(s) 207
Mox20I TGGCCA 1 cut(s) 1223
MroXI GAANNNNTTC 1 cut(s) 792
MscI TGGCCA 1 cut(s) 1223
MseI TTAA 3 cut(s) 528, 738, 1268
MslI CAYNNNNRTG 4 cut(s) 227, 1100, 1229, 1533
Msp20I TGGCCA 1 cut(s) 1223
MspA1I CMGCKG 1 cut(s) 1199
MspI CCGG 2 cut(s) 821, 1576
MspR9I CCNGG 7 cut(s) 74, 341, 365, 1390, 1415, 1577, 1635
MunI CAATTG 3 cut(s) 517, 567, 927
Mva1269I GAATGC 1 cut(s) 438
MvaI CCWGG 6 cut(s) 74, 341, 365, 1390, 1415, 1635
MvnI CGCG 2 cut(s) 285, 1363
MwoI GCNNNNNNNGC 7 cut(s) 110, 395, 464, 776, 1169, 1459, 1515
NciI CCSGG 1 cut(s) 1577
NcoI CCATGG 1 cut(s) 1534
NdeI CATATG 1 cut(s) 1523
NdeII GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
NlaIII CATG 6 cut(s) 172, 668, 779, 884, 1234, 1538
NlaIV GGNNCC 4 cut(s) 137, 251, 676, 1126
NmuCI GTSAC 1 cut(s) 857
NspI RCATGY 1 cut(s) 779
PaeI GCATGC 1 cut(s) 779
PaeR7I CTCGAG 1 cut(s) 296
PaqCI CACCTGC 1 cut(s) 911
PceI AGGCCT 2 cut(s) 580, 973
PctI GAATGC 1 cut(s) 438
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 7 cut(s) 994, 1118, 1136, 1182, 1297, 1311, 1378
PflFI GACNNNGTC 2 cut(s) 268, 1390
PfoI TCCNGGA 1 cut(s) 363
PleI GAGTC 2 cut(s) 377, 550
PpsI GAGTC 2 cut(s) 377, 550
Psp6I CCWGG 6 cut(s) 72, 339, 363, 1388, 1413, 1633
PspFI CCCAGC 3 cut(s) 245, 1168, 1550
PspGI CCWGG 6 cut(s) 72, 339, 363, 1388, 1413, 1633
PspN4I GGNNCC 4 cut(s) 137, 251, 676, 1126
PspPI GGNCC 7 cut(s) 59, 675, 965, 1125, 1247, 1386, 1451
PstNI CAGNNNCTG 1 cut(s) 1256
PsuI RGATCY 2 cut(s) 506, 523
PsyI GACNNNGTC 2 cut(s) 268, 1390
PvuII CAGCTG 1 cut(s) 1199
RseI CAYNNNNRTG 4 cut(s) 227, 1100, 1229, 1533
SaqAI TTAA 3 cut(s) 528, 738, 1268
Sau3AI GATC 8 cut(s) 96, 487, 506, 523, 637, 661, 1468, 1564
Sau96I GGNCC 7 cut(s) 59, 675, 965, 1125, 1247, 1386, 1451
SchI GAGTC 2 cut(s) 377, 551
ScrFI CCNGG 7 cut(s) 74, 341, 365, 1390, 1415, 1577, 1635
SduI GDGCHC 2 cut(s) 29, 254
SexAI ACCWGGT 1 cut(s) 1388
SfaNI GCATC 5 cut(s) 992, 1065, 1172, 1582, 1653
SfcI CTRYAG 1 cut(s) 475
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 3 cut(s) 59, 675, 1386
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 4 cut(s) 227, 1100, 1229, 1533
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SphI GCATGC 1 cut(s) 779
Sse9I AATT 7 cut(s) 517, 567, 592, 927, 1074, 1157, 1315
SseBI AGGCCT 2 cut(s) 580, 973
SsiI CCGC 6 cut(s) 38, 111, 125, 145, 1504, 1507
SspI AATATT 1 cut(s) 760
StuI AGGCCT 2 cut(s) 580, 973
StyD4I CCNGG 7 cut(s) 72, 339, 363, 1388, 1413, 1575, 1633
StyI CCWWGG 2 cut(s) 968, 1534
TaaI ACNGT 5 cut(s) 722, 784, 1150, 1219, 1513
TaiI ACGT 1 cut(s) 429
TaqI TCGA 6 cut(s) 297, 558, 610, 763, 827, 1309
TasI AATT 7 cut(s) 517, 567, 592, 927, 1074, 1157, 1315
TauI GCSGC 5 cut(s) 41, 113, 127, 1506, 1509
TfiI GAWTC 7 cut(s) 994, 1118, 1136, 1182, 1297, 1311, 1378
Tru1I TTAA 3 cut(s) 528, 738, 1268
Tru9I TTAA 3 cut(s) 528, 738, 1268
TseFI GTSAC 1 cut(s) 857
TseI GCWGC 5 cut(s) 260, 450, 725, 1199, 1542
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 4 cut(s) 185, 795, 804, 1709
TspGWI ACGGA 2 cut(s) 915, 1589
Tth111I GACNNNGTC 2 cut(s) 268, 1390
VpaK11BI GGWCC 3 cut(s) 59, 675, 1386
XceI RCATGY 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 1 cut(s) 1421
XhoI CTCGAG 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 1616
XmnI GAANNNNTTC 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.