Rw2G039130

(R)-mandelonitrile lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014093
Physical Location & Seq
Reverse (-)
64098587 .. 64101314
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039130.1

Sequence Viewer

Length: 1689 bp
ATGGCGGTGAGACCCGTTTTGCTCTCTCTACTTCTTCAGGCGTTTGCCTTCATGGTGGTGATCAATGCCGTCGTCGCTTCTCCAAGGCCGCCGTCTCGGAACCCACGCCGCCCTCTTCAAGAGCCAAGTTACCTGAAGTTTGTTTCCAACGCCACTGACTTCCCATTGGAGGACTACTATGACTACATTATCGTGGGAGGTGGCACTGCTGGGTGCCCTTTGGCTGCGACTCTGTCATCCAGGTTTCGGGTCCTCCTGCTGGAAAGAGGTGGTGTTGCTTATGGCAATCGAAACTTGATGACCCAGGAAGGTTTCTTGGCCACACTCATGGATGTCAACACCTTTGACTCTCCGACACAAGCCTTCACATCTGAAGATGGTGTTCCAAATGTCCGAGGACGCATTCTTGGAGGCAGCAGTGCCATAAATGCTGGTTTCTATAGCCGTGCAGATCACGAATTCTACAAAAGGTCCACTTTCAAATGGGATCTTAAAGTGGTGAATGAGTCCTATGAGTGGGTTGAAAGAGCAATTGTCTTCAGGCCTGAGCTTAGGAATTGGCAGTCAGCAGTTCGAGATGGCTTATTGCAAGCTGGTATTGATCCCTATAATGGGTATAGTTTTGATCATGTAGTGGGGACCAAGACTGGTGGTTCAACATTTGATACTTTGGGAAGGAGACACAGTGCTGCTGACCTTCTTAAATATGCAAAGCCATTGAATATTCGAGTTGCAGTGCATGCCACTGTGGAAAAAGTTCTGTTGGCTTCATCTACTTCATCTCCAGTGTCGAGGCAATCTGCTGTTGGAGTTATTTTTCGTGACAGAAGTGGGAGGTATCACCATGCTATGCTACGAAAACATGGAGAGGTCCTTCTCTCAGCTGGTGCAATTGGGACTCCACAGCTACTTCTTCTGAGTGGTATTGGCCCAAGGCCTTATCTTGCATCTTGGGGGATTCCAGTGGCTCATCATCTTCCTTATGTTGGGCAGTTTATGTTTGATAATCCCAGAAATGGCATCTCAATAGTGCCACCAATTCCACTGGAGCACTCACTCATTCAAGTGGTTGGCATTACTGAATCAGGGGCTTACATTGAATCAGCCTCTACTGTCATCCAATTTGCATCCCCAGCCCGCTCTGTGTTCCTTAGGACACCGTCTGCACCTCTATATCTTACTGTGGCCACCCTCATGGAGAAGATTGTCGGGCCAGTCTCTGCTGGTTCTTTAAGGCTGGCTTCAACTGATATCAACGTGAATCCTATTGTTCGATTCAATTACTTCAACAGCCCAGTGGATTTGCAAAGGTGTGTGAATGGGACGCGAAAGATTGGTGACATTCTCAGGACCAGGTCTATGGAGGATTTCAAGTTCCAGGGATGGTTTGGGAATAGAGATTTTAGGTTTATAGGGCCTGCATTGCCAGTTGATCAGTCTGACTTTGACGTAATGGCAGATTTCTGCCGCCGCACTGTAAGCACCATATGGCACTACCATGGGGGCTGCATTGCTGGGAAGGTGGTTGATGGTGATTTCCGGGTCATTGGTATAGATGCACTCCGTGTTGTTGATGGGTCTACTTTTGGTATATCACCAGGGACAAATCCTCAGGCTACCCTTCTGATGCTCGGAAGGTTGGTGTCCTCAAATCATGTTAGAAGTCCTTGTAGCTTTGTAGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

562

Amino Acids

61.59

Weight (kDa)

9.41

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 60 - 331 2.7e-18 GMC oxidoreductase
GMC_oxred_C PF05199 405 - 545 3.2e-26 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73050
fragaria_vesca FvH4_6g37470 FvH4_6g37470
malus_domestica MD09G1157800.v1.1 MD17G1143800.v1.1
prunus_persica Prupe.3G168200_v2.0.a1
pyrus_communis pycom17g13690
rosa_chinensis RchiOBHm_Chr2g0150311
rosa_laevigata RLG00000020486
rosa_multiflora Rmu_sc0002179.1_g000001 Rmu_sc0025680.1_g000002
rosa_roxburghii Rroxscaffold_2G00097300
rosa_rugosa Rorug02G0420400
rosa_samantha Rh2AG479400 Rh2BG491700 Rh2DG500500
rosa_wichuraiana Rw2G039130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 1140
AccI GTMKAC 1 cut(s) 1580
AccII CGCG 1 cut(s) 1327
AciI CCGC 6 cut(s) 5, 89, 109, 1138, 1468, 1471
AclWI GGATC 2 cut(s) 495, 596
AcoI YGGCCR 2 cut(s) 318, 1185
AcsI RAATTY 1 cut(s) 458
AcuI CTGAAG 4 cut(s) 20, 155, 393, 523
AdeI CACNNNGTG 1 cut(s) 1565
AfiI CCNNNNNNNGG 8 cut(s) 169, 246, 259, 516, 611, 612, 986, 1016
AjnI CCWGG 5 cut(s) 239, 303, 1352, 1377, 1597
AloI GAACNNNNNNTCC 2 cut(s) 1358, 1390
AluBI AGCT 6 cut(s) 550, 593, 884, 907, 1674, 1682
AluI AGCT 6 cut(s) 550, 593, 884, 907, 1674, 1682
Alw21I GWGCWC 1 cut(s) 1053
Alw26I GTCTC 4 cut(s) 4, 99, 673, 1222
AlwI GGATC 2 cut(s) 495, 596
AlwNI CAGNNNCTG 1 cut(s) 1220
AoxI GGCC 8 cut(s) 86, 318, 542, 928, 935, 1185, 1211, 1415
ApeKI GCWGC 4 cut(s) 224, 414, 689, 1506
ApoI RAATTY 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 756
AspS9I GGNCC 8 cut(s) 250, 471, 639, 871, 929, 1211, 1350, 1415
AsuC2I CCSGG 1 cut(s) 1541
AsuHPI GGTGA 7 cut(s) 19, 70, 511, 833, 1349, 1544, 1587
AvaII GGWCC 5 cut(s) 250, 471, 639, 871, 1350
AxyI CCTNAGG 2 cut(s) 1151, 1611
BaeGI GKGCMC 1 cut(s) 218
BalI TGGCCA 2 cut(s) 320, 1187
BanI GGYRCC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 1053
BbvI GCAGC 4 cut(s) 211, 426, 676, 1493
BccI CCATC 5 cut(s) 371, 572, 1377, 1523, 1568
BceAI ACGGC 3 cut(s) 53, 76, 429
BciT130I CCWGG 5 cut(s) 241, 305, 1354, 1379, 1599
BclI TGATCA 3 cut(s) 60, 625, 1432
BcnI CCSGG 1 cut(s) 1541
BcoDI GTCTC 4 cut(s) 4, 99, 673, 1222
BfmI CTRYAG 1 cut(s) 439
BisI GCNGC 8 cut(s) 89, 109, 225, 415, 690, 1468, 1471, 1507
BlsI GCNGC 8 cut(s) 90, 110, 226, 416, 691, 1469, 1472, 1508
Bme1390I CCNGG 6 cut(s) 241, 305, 1354, 1379, 1541, 1599
Bme18I GGWCC 5 cut(s) 250, 471, 639, 871, 1350
BmgT120I GGNCC 8 cut(s) 250, 471, 639, 871, 929, 1211, 1350, 1415
BmiI GGNNCC 4 cut(s) 101, 215, 251, 640
BmrFI CCNGG 6 cut(s) 241, 305, 1354, 1379, 1541, 1599
BmrI ACTGGG 1 cut(s) 1289
BmsI GCATC 5 cut(s) 956, 1029, 1136, 1546, 1617
BmuI ACTGGG 1 cut(s) 1289
BpiI GAAGAC 1 cut(s) 529
BpmI CTGGAG 2 cut(s) 768, 1067
Bpu10I CCTNAGC 2 cut(s) 546, 551
BpuMI CCSGG 1 cut(s) 1541
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 7 cut(s) 83, 303, 394, 932, 1378, 1498, 1598
BsaXI ACNNNNNCTCC 4 cut(s) 670, 700, 766, 796
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 246, 259, 516, 611, 612, 986, 1016
Bse1I ACTGG 7 cut(s) 652, 785, 962, 1050, 1214, 1295, 1427
Bse21I CCTNAGG 2 cut(s) 1151, 1611
Bse3DI GCAATG 2 cut(s) 1421, 1509
BseBI CCWGG 5 cut(s) 241, 305, 1354, 1379, 1599
BseDI CCNNGG 7 cut(s) 83, 303, 394, 932, 1378, 1498, 1598
BseGI GGATG 5 cut(s) 236, 337, 1116, 1127, 1388
BseLI CCNNNNNNNGG 8 cut(s) 169, 246, 259, 516, 611, 612, 986, 1016
BseMI GCAATG 2 cut(s) 1421, 1509
BseMII CTCAG 5 cut(s) 537, 894, 908, 1360, 1625
BseNI ACTGG 7 cut(s) 652, 785, 962, 1050, 1214, 1295, 1427
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 4 cut(s) 211, 426, 676, 1493
BseYI CCCAGC 3 cut(s) 209, 1132, 1514
BsgI GTGCAG 2 cut(s) 468, 1149
Bsh1236I CGCG 1 cut(s) 1327
BshFI GGCC 8 cut(s) 88, 320, 544, 930, 937, 1187, 1213, 1417
BshNI GGYRCC 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 1053
BsiSI CCGG 1 cut(s) 1540
BslFI GGGAC 4 cut(s) 652, 910, 1336, 1615
BslI CCNNNNNNNGG 8 cut(s) 169, 246, 259, 516, 611, 612, 986, 1016
BsmAI GTCTC 4 cut(s) 4, 99, 673, 1222
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 4 cut(s) 652, 910, 1336, 1615
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 8 cut(s) 88, 320, 544, 930, 937, 1187, 1213, 1417
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 2 cut(s) 218, 1053
Bsp143I GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
Bsp19I CCATGG 1 cut(s) 1498
BspACI CCGC 6 cut(s) 5, 89, 109, 1138, 1468, 1471
BspANI GGCC 8 cut(s) 88, 320, 544, 930, 937, 1187, 1213, 1417
BspCNI CTCAG 5 cut(s) 538, 893, 909, 1359, 1624
BspFNI CGCG 1 cut(s) 1327
BspLI GGNNCC 4 cut(s) 101, 215, 251, 640
BspPI GGATC 2 cut(s) 495, 596
BspT107I GGYRCC 1 cut(s) 213
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 1 cut(s) 1140
BsrDI GCAATG 2 cut(s) 1421, 1509
BsrI ACTGG 7 cut(s) 652, 785, 962, 1050, 1214, 1295, 1427
BssECI CCNNGG 7 cut(s) 83, 303, 394, 932, 1378, 1498, 1598
BssMI GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
BssT1I CCWWGG 3 cut(s) 83, 932, 1498
Bst2UI CCWGG 5 cut(s) 241, 305, 1354, 1379, 1599
Bst4CI ACNGT 6 cut(s) 686, 748, 1114, 1161, 1183, 1477
Bst6I CTCTTC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 740
BstC8I GCNNGC 5 cut(s) 591, 741, 1138, 1239, 1419
BstDEI CTNAG 7 cut(s) 546, 551, 880, 917, 1151, 1346, 1611
BstDSI CCRYGG 1 cut(s) 1498
BstF5I GGATG 5 cut(s) 236, 337, 1116, 1127, 1388
BstFNI CGCG 1 cut(s) 1327
BstKTI GATC 6 cut(s) 63, 454, 490, 604, 628, 1435
BstMAI GTCTC 4 cut(s) 4, 99, 673, 1222
BstMBI GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
BstMWI GCNNNNNNNGC 6 cut(s) 74, 428, 740, 1133, 1423, 1479
BstNI CCWGG 5 cut(s) 241, 305, 1354, 1379, 1599
BstNSI RCATGY 1 cut(s) 743
BstSCI CCNGG 6 cut(s) 239, 303, 1352, 1377, 1539, 1597
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 1327
BstV1I GCAGC 4 cut(s) 211, 426, 676, 1493
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 487
BstXI CCANNNNNNTGG 3 cut(s) 328, 1195, 1360
BstYI RGATCY 1 cut(s) 487
Bsu36I CCTNAGG 2 cut(s) 1151, 1611
BsuRI GGCC 8 cut(s) 88, 320, 544, 930, 937, 1187, 1213, 1417
BtgI CCRYGG 1 cut(s) 1498
BtsCI GGATG 5 cut(s) 236, 337, 1116, 1127, 1388
BtsI GCAGTG 3 cut(s) 204, 424, 741
Cac8I GCNNGC 5 cut(s) 591, 741, 1138, 1239, 1419
CaiI CAGNNNCTG 1 cut(s) 1220
Cfr13I GGNCC 8 cut(s) 250, 471, 639, 871, 929, 1211, 1350, 1415
CseI GACGC 2 cut(s) 408, 1333
CsiI ACCWGGT 1 cut(s) 1352
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviAII CATG 9 cut(s) 52, 328, 629, 740, 845, 863, 1195, 1499, 1655
DdeI CTNAG 7 cut(s) 546, 551, 880, 917, 1151, 1346, 1611
DpnI GATC 6 cut(s) 62, 453, 489, 603, 627, 1434
DpnII GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
DraIII CACNNNGTG 1 cut(s) 1565
EaeI YGGCCR 2 cut(s) 318, 1185
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 83, 932, 1498
Eco147I AGGCCT 2 cut(s) 544, 937
Eco31I GGTCTC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 1252
Eco47I GGWCC 5 cut(s) 250, 471, 639, 871, 1350
Eco57I CTGAAG 4 cut(s) 20, 155, 393, 523
Eco81I CCTNAGG 2 cut(s) 1151, 1611
EcoO109I RGGNCCY 3 cut(s) 250, 871, 1415
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 5 cut(s) 239, 303, 1352, 1377, 1597
EcoRV GATATC 1 cut(s) 1252
EcoT14I CCWWGG 3 cut(s) 83, 932, 1498
ErhI CCWWGG 3 cut(s) 83, 932, 1498
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 9 cut(s) 55, 331, 632, 743, 848, 866, 1198, 1502, 1658
FalI AAGNNNNNCTT 4 cut(s) 460, 492, 1225, 1257
FaqI GGGAC 4 cut(s) 652, 910, 1336, 1615
FatI CATG 9 cut(s) 51, 327, 628, 739, 844, 862, 1194, 1498, 1654
FauI CCCGC 1 cut(s) 1145
FauNDI CATATG 1 cut(s) 1487
FbaI TGATCA 3 cut(s) 60, 625, 1432
FblI GTMKAC 1 cut(s) 1580
Fnu4HI GCNGC 8 cut(s) 89, 109, 225, 415, 690, 1468, 1471, 1507
FokI GGATG 5 cut(s) 223, 344, 1103, 1114, 1395
Fsp4HI GCNGC 8 cut(s) 89, 109, 225, 415, 690, 1468, 1471, 1507
GluI GCNGC 8 cut(s) 89, 109, 225, 415, 690, 1468, 1471, 1507
GsaI CCCAGC 3 cut(s) 213, 1136, 1518
GsuI CTGGAG 2 cut(s) 768, 1067
HaeIII GGCC 8 cut(s) 88, 320, 544, 930, 937, 1187, 1213, 1417
HapII CCGG 1 cut(s) 1540
HgaI GACGC 2 cut(s) 408, 1333
Hin1II CATG 9 cut(s) 55, 331, 632, 743, 848, 866, 1198, 1502, 1658
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 9 cut(s) 229, 347, 506, 898, 958, 1082, 1100, 1261, 1275
HpaII CCGG 1 cut(s) 1540
HphI GGTGA 7 cut(s) 19, 70, 511, 833, 1349, 1544, 1587
Hpy166II GTNNAC 3 cut(s) 337, 474, 1581
Hpy188I TCNGA 8 cut(s) 99, 354, 373, 395, 918, 1441, 1626, 1634
Hpy188III TCNNGA 5 cut(s) 119, 455, 575, 821, 1348
Hpy8I GTNNAC 3 cut(s) 337, 474, 1581
Hpy99I CGWCG 2 cut(s) 74, 77
HpyAV CCTTC 9 cut(s) 58, 302, 373, 669, 707, 884, 1513, 1629, 1631
HpyCH4III ACNGT 6 cut(s) 686, 748, 1114, 1161, 1183, 1477
HpyCH4IV ACGT 2 cut(s) 1257, 1449
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 428, 740, 1133, 1423, 1479
HpyF3I CTNAG 7 cut(s) 546, 551, 880, 917, 1151, 1346, 1611
HpySE526I ACGT 2 cut(s) 1257, 1449
Hsp92II CATG 9 cut(s) 55, 331, 632, 743, 848, 866, 1198, 1502, 1658
Ksp22I TGATCA 3 cut(s) 60, 625, 1432
Kzo9I GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
LmnI GCTCC 1 cut(s) 1048
Lsp1109I GCAGC 4 cut(s) 211, 426, 676, 1493
LweI GCATC 5 cut(s) 956, 1029, 1136, 1546, 1617
MabI ACCWGGT 1 cut(s) 1352
MaeII ACGT 2 cut(s) 1257, 1449
MaeIII GTNAC 3 cut(s) 128, 821, 1337
MalI GATC 6 cut(s) 62, 453, 489, 603, 627, 1434
MbiI CCGCTC 1 cut(s) 1140
MboI GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
MboII GAAGA 7 cut(s) 26, 107, 386, 529, 905, 968, 1213
MfeI CAATTG 2 cut(s) 531, 891
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 2 cut(s) 218, 1053
MlsI TGGCCA 2 cut(s) 320, 1187
MluCI AATT 8 cut(s) 458, 531, 556, 891, 1038, 1121, 1279, 1684
MluNI TGGCCA 2 cut(s) 320, 1187
MlyI GAGTC 4 cut(s) 223, 341, 515, 892
MmeI TCCRAC 3 cut(s) 171, 377, 787
Mox20I TGGCCA 2 cut(s) 320, 1187
MroXI GAANNNNTTC 1 cut(s) 756
MscI TGGCCA 2 cut(s) 320, 1187
MseI TTAA 3 cut(s) 492, 702, 1232
MslI CAYNNNNRTG 6 cut(s) 56, 191, 326, 1064, 1193, 1497
Msp20I TGGCCA 2 cut(s) 320, 1187
MspA1I CMGCKG 1 cut(s) 884
MspI CCGG 1 cut(s) 1540
MspR9I CCNGG 6 cut(s) 241, 305, 1354, 1379, 1541, 1599
MunI CAATTG 2 cut(s) 531, 891
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 5 cut(s) 241, 305, 1354, 1379, 1599
MvnI CGCG 1 cut(s) 1327
MwoI GCNNNNNNNGC 6 cut(s) 74, 428, 740, 1133, 1423, 1479
NciI CCSGG 1 cut(s) 1541
NcoI CCATGG 1 cut(s) 1498
NdeI CATATG 1 cut(s) 1487
NdeII GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
NlaIII CATG 9 cut(s) 55, 331, 632, 743, 848, 866, 1198, 1502, 1658
NlaIV GGNNCC 4 cut(s) 101, 215, 251, 640
NmuCI GTSAC 2 cut(s) 821, 1337
NspI RCATGY 1 cut(s) 743
PaeI GCATGC 1 cut(s) 743
PceI AGGCCT 2 cut(s) 544, 937
PctI GAATGC 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 5 cut(s) 958, 1082, 1100, 1261, 1275
PflFI GACNNNGTC 3 cut(s) 232, 1159, 1354
PkrI GCNGC 8 cut(s) 90, 110, 226, 416, 691, 1469, 1472, 1508
PleI GAGTC 4 cut(s) 223, 341, 514, 892
PpsI GAGTC 4 cut(s) 223, 341, 514, 892
PpuMI RGGWCCY 2 cut(s) 250, 871
Psp5II RGGWCCY 2 cut(s) 250, 871
Psp6I CCWGG 5 cut(s) 239, 303, 1352, 1377, 1597
PspFI CCCAGC 3 cut(s) 209, 1132, 1514
PspGI CCWGG 5 cut(s) 239, 303, 1352, 1377, 1597
PspN4I GGNNCC 4 cut(s) 101, 215, 251, 640
PspPI GGNCC 8 cut(s) 250, 471, 639, 871, 929, 1211, 1350, 1415
PspPPI RGGWCCY 2 cut(s) 250, 871
PstNI CAGNNNCTG 1 cut(s) 1220
PsuI RGATCY 1 cut(s) 487
PsyI GACNNNGTC 3 cut(s) 232, 1159, 1354
PvuII CAGCTG 1 cut(s) 884
RseI CAYNNNNRTG 6 cut(s) 56, 191, 326, 1064, 1193, 1497
SaqAI TTAA 3 cut(s) 492, 702, 1232
SatI GCNGC 8 cut(s) 89, 109, 225, 415, 690, 1468, 1471, 1507
Sau3AI GATC 6 cut(s) 60, 451, 487, 601, 625, 1432
Sau96I GGNCC 8 cut(s) 250, 471, 639, 871, 929, 1211, 1350, 1415
SchI GAGTC 4 cut(s) 223, 341, 515, 892
ScrFI CCNGG 6 cut(s) 241, 305, 1354, 1379, 1541, 1599
SduI GDGCHC 2 cut(s) 218, 1053
SexAI ACCWGGT 1 cut(s) 1352
SfaNI GCATC 5 cut(s) 956, 1029, 1136, 1546, 1617
SfcI CTRYAG 1 cut(s) 439
SinI GGWCC 5 cut(s) 250, 471, 639, 871, 1350
SmiMI CAYNNNNRTG 6 cut(s) 56, 191, 326, 1064, 1193, 1497
SphI GCATGC 1 cut(s) 743
Sse9I AATT 8 cut(s) 458, 531, 556, 891, 1038, 1121, 1279, 1684
SseBI AGGCCT 2 cut(s) 544, 937
SsiI CCGC 6 cut(s) 5, 89, 109, 1138, 1468, 1471
SspI AATATT 1 cut(s) 724
StuI AGGCCT 2 cut(s) 544, 937
StyD4I CCNGG 6 cut(s) 239, 303, 1352, 1377, 1539, 1597
StyI CCWWGG 3 cut(s) 83, 932, 1498
TaaI ACNGT 6 cut(s) 686, 748, 1114, 1161, 1183, 1477
TaiI ACGT 2 cut(s) 1260, 1452
TaqI TCGA 5 cut(s) 289, 574, 727, 791, 1273
TasI AATT 8 cut(s) 458, 531, 556, 891, 1038, 1121, 1279, 1684
TauI GCSGC 4 cut(s) 91, 111, 1470, 1473
TfiI GAWTC 5 cut(s) 958, 1082, 1100, 1261, 1275
Tru1I TTAA 3 cut(s) 492, 702, 1232
Tru9I TTAA 3 cut(s) 492, 702, 1232
TseFI GTSAC 2 cut(s) 821, 1337
TseI GCWGC 4 cut(s) 224, 414, 689, 1506
Tsp45I GTSAC 2 cut(s) 821, 1337
TspDTI ATGAA 3 cut(s) 40, 759, 768
TspGWI ACGGA 1 cut(s) 1553
Tth111I GACNNNGTC 3 cut(s) 232, 1159, 1354
VpaK11BI GGWCC 5 cut(s) 250, 471, 639, 871, 1350
XapI RAATTY 1 cut(s) 458
XceI RCATGY 1 cut(s) 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 1385
XmiI GTMKAC 1 cut(s) 1580
XmnI GAANNNNTTC 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.