MD17G1143800.v1.1

(R)-mandelonitrile lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014093
Physical Location & Seq
Forward (+)
12967231 .. 12969351
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1143800.v1.1.491

Sequence Viewer

Length: 1653 bp
ATGTCGCATGCTTTCAATTCTTGGAAATTTGTATTTTCAGTGAATTTTCTGGGTTATCAACCAGAGGATAATTCTTCTCTGTTGCCTAGTTACCTCAAATTTGTTGTCAACGCCACCGATTTTCCAACAGAAGATTATTACGACTACATTATTGTAGGAGGTGGCACTGCTGGGTGCCCCTTGGCTGCGACATTATCATCTAGATTCCGAGTGCTCCTGCTCGAACGGGGTGGGGTTGCTTATGGAAATCGAAACTTGATGACCAGAGAAGGTTTCTTGGCCACACTCATGGATGTCAACTCCTTTGACTCACCTACTCAAGCCTTCACATCTGAAGAAGGGGTCGCAAATGTCCGAGGCCGCATTCTTGGAGGCAGCAGTGCCATAAATGCTGGTTTCTACAGCCGTGCAGATCAGGACTTTTACACCAAAACCAGTGTCGATTGGGATCCCCGAATGGTGAATGAGTCGTACGAGTGGGTTGAAAGGGCAATTGTGTTTAGGCCGGAGCTGAGGACTTGGCAATCGGCAGTACGAGATGGGTTATTGGAAGCTGGTGTTGATCCCTACAACGGGTTCGATTTGGAACATGCGGTGGGGACCAAGATTGGTGGTTCGACATTTGACACTTCGGGAAGGAGACACAGTGCCGCAGATCTTCTCAAGTATGCAAATCCACTTAACATCAAAGTTGTGACTCATGCAAGTGTGGAGAGGATTCTGTTGGCTTCAACTATGCCGTCTCCAGCATCAAGGCAGTCTGCAGCGGGTGTTGTTTTTCGTGACAGAGTCGGGAGGTATCACCACGCTGTATTACGTGAACATGGTGAGGTTATTCTATCAGCTGGTGCCATCGGAAGTCCACAGCTGCTTCTGCTGAGTGGTATTGGCCCAAGGCCTTATCTTTCGTCATGGGGGATTCCAGTGGCTCATCATCTTCCTTATGTTGGGCAATATCTCTATGATAACCCAAGAAATGGCATCTCAATTGTGCCACCATTTCCACTGGAGCACTCACTGATACAAGTTGTGGGAATTACTGAATCAGGAGCTTACATTGAAGCAGCCTCCAATGTTATCCCTTTTGCATCACCTGCAATGTCTGTGTTCATTCGGACACCATCCGCACCTCTCTACCTCACTGTGGCAACCCTCATGGAGAAGACCATTGGGCCAGCCTCAGCTGGTTCTCTGAGGTTGGCTTCAACAAATGTCAGGGTGAACCCAATTGTTCGATTCAACTACTTCAGCAACCCGGTGGATGTGCACAGGTGTGTGAATGGGACGCGCAAGATTGGTGATATACTCAAGACCAGGTCTATGGAGGATTTCAAGTTCCAGGGCTGGTTTGGGAGAAAAGACTTTAGGTTTGTGGGACCTGCATTGCCTGTAGACCAATCGAACTATGAGCTGATGGCAGATTTCTGTCGCCGCACTGTTAGCACCATATGGCATTACCATGGGGGCTGCATTTTGGGGAAGGTGGTTGATGGTGATTTCCGGGTCATTGGTACTGATGCTCTCAGAGTTGTCGATGGATCAACATTAGGTATATCACCTGGGACAAATCCTCAGGCTACTCTTATGATGCTTGGGAGATACGTTGGGCTGAGGATAATGAAAGACCGAAAGGAATATAAGATGATCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

551

Amino Acids

60.57

Weight (kDa)

8.49

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 47 - 323 3.7e-21 GMC oxidoreductase
GMC_oxred_C PF05199 392 - 532 3.7e-27 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73050
fragaria_vesca FvH4_6g37470 FvH4_6g37470
malus_domestica MD09G1157800.v1.1 MD17G1143800.v1.1
prunus_persica Prupe.3G168200_v2.0.a1
pyrus_communis pycom17g13690
rosa_chinensis RchiOBHm_Chr2g0150311
rosa_laevigata RLG00000020486
rosa_multiflora Rmu_sc0002179.1_g000001 Rmu_sc0025680.1_g000002
rosa_roxburghii Rroxscaffold_2G00097300
rosa_rugosa Rorug02G0420400
rosa_samantha Rh2AG479400 Rh2BG491700 Rh2DG500500
rosa_wichuraiana Rw2G039130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1102
Acc36I ACCTGC 2 cut(s) 1102, 1387
AccB1I GGYRCC 2 cut(s) 174, 848
AccB7I CCANNNNNTGG 1 cut(s) 977
AccI GTMKAC 1 cut(s) 1392
AccII CGCG 1 cut(s) 1288
AciI CCGC 6 cut(s) 361, 593, 651, 767, 1125, 1432
AclWI GGATC 4 cut(s) 443, 456, 557, 1546
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 3 cut(s) 26, 43, 98
AcuI CTGAAG 2 cut(s) 354, 1231
AfaI GTAC 3 cut(s) 473, 534, 1513
AfiI CCNNNNNNNGG 5 cut(s) 572, 573, 947, 977, 1144
AgsI TTSAA 7 cut(s) 16, 485, 732, 1061, 1206, 1240, 1333
AjnI CCWGG 3 cut(s) 1313, 1338, 1558
AleI CACNNNNGTG 1 cut(s) 1272
AloI GAACNNNNNNTCC 2 cut(s) 1319, 1351
AluBI AGCT 7 cut(s) 511, 554, 845, 868, 1052, 1184, 1411
AluI AGCT 7 cut(s) 511, 554, 845, 868, 1052, 1184, 1411
Alw21I GWGCWC 3 cut(s) 216, 1014, 1269
Alw26I GTCTC 2 cut(s) 634, 747
Alw44I GTGCAC 1 cut(s) 1265
AlwI GGATC 4 cut(s) 443, 456, 557, 1546
AoxI GGCC 6 cut(s) 279, 358, 503, 889, 896, 1172
ApaLI GTGCAC 1 cut(s) 1265
ApeKI GCWGC 6 cut(s) 185, 375, 764, 868, 1064, 1467
ApoI RAATTY 3 cut(s) 26, 43, 98
ArsI GACNNNNNNTTYG 6 cut(s) 90, 122, 423, 455, 1352, 1384
AspLEI GCGC 1 cut(s) 1290
AspS9I GGNCC 4 cut(s) 600, 890, 1172, 1376
AsuC2I CCSGG 2 cut(s) 1256, 1502
AsuHPI GGTGA 9 cut(s) 303, 472, 794, 839, 1083, 1231, 1310, 1505, 1548
AvaII GGWCC 2 cut(s) 600, 1376
AxyI CCTNAGG 1 cut(s) 1572
BaeGI GKGCMC 2 cut(s) 179, 1269
BalI TGGCCA 1 cut(s) 281
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 2 cut(s) 174, 848
BbsI GAAGAC 1 cut(s) 1169
Bbv12I GWGCWC 3 cut(s) 216, 1014, 1269
BbvCI CCTCAGC 3 cut(s) 512, 1180, 1610
BbvI GCAGC 6 cut(s) 172, 387, 776, 855, 1076, 1454
BccI CCATC 6 cut(s) 533, 860, 1129, 1408, 1484, 1529
BceAI ACGGC 2 cut(s) 390, 724
BciT130I CCWGG 3 cut(s) 1315, 1340, 1560
BcnI CCSGG 2 cut(s) 1256, 1502
BcoDI GTCTC 2 cut(s) 634, 747
BfaI CTAG 2 cut(s) 87, 201
BfmI CTRYAG 3 cut(s) 400, 762, 1389
BfuAI ACCTGC 2 cut(s) 1102, 1387
BglII AGATCT 1 cut(s) 655
BisI GCNGC 9 cut(s) 186, 361, 376, 651, 765, 869, 1065, 1432, 1468
BlsI GCNGC 9 cut(s) 187, 362, 377, 652, 766, 870, 1066, 1433, 1469
Bme1390I CCNGG 5 cut(s) 1256, 1315, 1340, 1502, 1560
Bme18I GGWCC 2 cut(s) 600, 1376
BmgT120I GGNCC 4 cut(s) 600, 890, 1172, 1376
BmiI GGNNCC 5 cut(s) 176, 450, 601, 850, 1377
BmrFI CCNGG 5 cut(s) 1256, 1315, 1340, 1502, 1560
BmsI GCATC 5 cut(s) 758, 990, 1097, 1507, 1578
BpiI GAAGAC 1 cut(s) 1169
BpmI CTGGAG 2 cut(s) 729, 1028
Bpu10I CCTNAGC 3 cut(s) 512, 1180, 1610
BpuEI CTTGAG 3 cut(s) 303, 647, 1292
BpuMI CCSGG 2 cut(s) 1256, 1502
BsaAI YACGTR 1 cut(s) 818
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 6 cut(s) 180, 355, 893, 1339, 1459, 1559
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 5 cut(s) 572, 573, 947, 977, 1144
Bse1I ACTGG 3 cut(s) 435, 923, 1011
Bse21I CCTNAGG 1 cut(s) 1572
Bse3DI GCAATG 2 cut(s) 1104, 1382
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 3 cut(s) 1315, 1340, 1560
BseDI CCNNGG 6 cut(s) 180, 355, 893, 1339, 1459, 1559
BseGI GGATG 3 cut(s) 298, 1121, 1267
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 5 cut(s) 572, 573, 947, 977, 1144
BseMI GCAATG 2 cut(s) 1104, 1382
BseMII CTCAG 7 cut(s) 503, 869, 1184, 1194, 1537, 1586, 1601
BseNI ACTGG 3 cut(s) 435, 923, 1011
BseSI GKGCMC 2 cut(s) 179, 1269
BseXI GCAGC 6 cut(s) 172, 387, 776, 855, 1076, 1454
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 1288
BshFI GGCC 6 cut(s) 281, 360, 505, 891, 898, 1174
BshNI GGYRCC 2 cut(s) 174, 848
BsiHKAI GWGCWC 3 cut(s) 216, 1014, 1269
BsiSI CCGG 3 cut(s) 506, 1256, 1501
BsiWI CGTACG 1 cut(s) 471
BslFI GGGAC 4 cut(s) 613, 1297, 1389, 1576
BslI CCNNNNNNNGG 5 cut(s) 572, 573, 947, 977, 1144
BsmAI GTCTC 2 cut(s) 634, 747
BsmBI CGTCTC 1 cut(s) 747
BsmFI GGGAC 4 cut(s) 613, 1297, 1389, 1576
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 6 cut(s) 281, 360, 505, 891, 898, 1174
Bsp1286I GDGCHC 4 cut(s) 179, 216, 1014, 1269
Bsp143I GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
Bsp19I CCATGG 1 cut(s) 1459
BspACI CCGC 6 cut(s) 361, 593, 651, 767, 1125, 1432
BspANI GGCC 6 cut(s) 281, 360, 505, 891, 898, 1174
BspCNI CTCAG 7 cut(s) 504, 870, 1185, 1193, 1536, 1585, 1602
BspFNI CGCG 1 cut(s) 1288
BspLI GGNNCC 5 cut(s) 176, 450, 601, 850, 1377
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 2 cut(s) 1102, 1387
BspPI GGATC 4 cut(s) 443, 456, 557, 1546
BspT107I GGYRCC 2 cut(s) 174, 848
BsrDI GCAATG 2 cut(s) 1104, 1382
BsrI ACTGG 3 cut(s) 435, 923, 1011
BssECI CCNNGG 6 cut(s) 180, 355, 893, 1339, 1459, 1559
BssMI GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
BssT1I CCWWGG 3 cut(s) 180, 893, 1459
Bst2UI CCWGG 3 cut(s) 1315, 1340, 1560
Bst4CI ACNGT 3 cut(s) 647, 1144, 1438
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstBAI YACGTR 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 9, 1176
BstDEI CTNAG 7 cut(s) 512, 878, 1180, 1193, 1523, 1572, 1610
BstDSI CCRYGG 1 cut(s) 1459
BstF5I GGATG 3 cut(s) 298, 1121, 1267
BstFNI CGCG 1 cut(s) 1288
BstHHI GCGC 1 cut(s) 1290
BstKTI GATC 6 cut(s) 415, 451, 565, 658, 1541, 1647
BstMAI GTCTC 2 cut(s) 634, 747
BstMBI GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
BstMWI GCNNNNNNNGC 4 cut(s) 389, 874, 1094, 1440
BstNI CCWGG 3 cut(s) 1315, 1340, 1560
BstNSI RCATGY 2 cut(s) 11, 593
BstSCI CCNGG 5 cut(s) 1254, 1313, 1338, 1500, 1558
BstSFI CTRYAG 3 cut(s) 400, 762, 1389
BstSLI GKGCMC 2 cut(s) 179, 1269
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 6 cut(s) 172, 387, 776, 855, 1076, 1454
BstV2I GAAGAC 1 cut(s) 1169
BstX2I RGATCY 2 cut(s) 448, 655
BstXI CCANNNNNNTGG 2 cut(s) 289, 1321
BstYI RGATCY 2 cut(s) 448, 655
Bsu36I CCTNAGG 1 cut(s) 1572
BsuRI GGCC 6 cut(s) 281, 360, 505, 891, 898, 1174
BtgI CCRYGG 1 cut(s) 1459
BtsCI GGATG 3 cut(s) 298, 1121, 1267
BtsI GCAGTG 2 cut(s) 165, 385
BveI ACCTGC 2 cut(s) 1102, 1387
Cac8I GCNNGC 2 cut(s) 9, 1176
CfoI GCGC 1 cut(s) 1290
Cfr13I GGNCC 4 cut(s) 600, 890, 1172, 1376
CseI GACGC 1 cut(s) 1294
CsiI ACCWGGT 1 cut(s) 1313
Csp6I GTAC 3 cut(s) 472, 533, 1512
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 8 cut(s) 8, 289, 590, 701, 824, 912, 1156, 1460
CviQI GTAC 3 cut(s) 472, 533, 1512
DdeI CTNAG 7 cut(s) 512, 878, 1180, 1193, 1523, 1572, 1610
DpnI GATC 6 cut(s) 414, 450, 564, 657, 1540, 1646
DpnII GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
EaeI YGGCCR 1 cut(s) 279
Eco130I CCWWGG 3 cut(s) 180, 893, 1459
Eco147I AGGCCT 1 cut(s) 898
Eco47I GGWCC 2 cut(s) 600, 1376
Eco57I CTGAAG 2 cut(s) 354, 1231
Eco81I CCTNAGG 1 cut(s) 1572
EcoO109I RGGNCCY 1 cut(s) 1376
EcoRII CCWGG 3 cut(s) 1313, 1338, 1558
EcoT14I CCWWGG 3 cut(s) 180, 893, 1459
ErhI CCWWGG 3 cut(s) 180, 893, 1459
Esp3I CGTCTC 1 cut(s) 747
FaeI CATG 8 cut(s) 11, 292, 593, 704, 827, 915, 1159, 1463
FaqI GGGAC 4 cut(s) 613, 1297, 1389, 1576
FatI CATG 8 cut(s) 7, 288, 589, 700, 823, 911, 1155, 1459
FauI CCCGC 1 cut(s) 760
FauNDI CATATG 1 cut(s) 1448
FblI GTMKAC 1 cut(s) 1392
Fnu4HI GCNGC 9 cut(s) 186, 361, 376, 651, 765, 869, 1065, 1432, 1468
FokI GGATG 3 cut(s) 305, 1108, 1274
Fsp4HI GCNGC 9 cut(s) 186, 361, 376, 651, 765, 869, 1065, 1432, 1468
FspBI CTAG 2 cut(s) 87, 201
GlaI GCGC 1 cut(s) 1289
GluI GCNGC 9 cut(s) 186, 361, 376, 651, 765, 869, 1065, 1432, 1468
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 2 cut(s) 729, 1028
HaeIII GGCC 6 cut(s) 281, 360, 505, 891, 898, 1174
HapII CCGG 3 cut(s) 506, 1256, 1501
HgaI GACGC 1 cut(s) 1294
HhaI GCGC 1 cut(s) 1290
Hin1II CATG 8 cut(s) 11, 292, 593, 704, 827, 915, 1159, 1463
Hin6I GCGC 1 cut(s) 1288
HinP1I GCGC 1 cut(s) 1288
HincII GTYRAC 2 cut(s) 109, 298
HindII GTYRAC 2 cut(s) 109, 298
HinfI GANTC 9 cut(s) 204, 308, 467, 697, 718, 789, 919, 1043, 1236
HpaII CCGG 3 cut(s) 506, 1256, 1501
HphI GGTGA 9 cut(s) 303, 472, 794, 839, 1083, 1231, 1310, 1505, 1548
Hpy166II GTNNAC 7 cut(s) 109, 298, 821, 863, 1222, 1267, 1393
Hpy188I TCNGA 7 cut(s) 209, 334, 356, 857, 1116, 1194, 1526
Hpy188III TCNNGA 7 cut(s) 201, 416, 633, 782, 793, 1047, 1309
Hpy8I GTNNAC 7 cut(s) 109, 298, 821, 863, 1222, 1267, 1393
HpyAV CCTTC 5 cut(s) 263, 332, 334, 630, 1474
HpyCH4III ACNGT 3 cut(s) 647, 1144, 1438
HpyCH4IV ACGT 2 cut(s) 817, 1602
HpyCH4V TGCA 9 cut(s) 410, 671, 704, 764, 1088, 1097, 1267, 1382, 1470
HpyF10VI GCNNNNNNNGC 4 cut(s) 389, 874, 1094, 1440
HpyF3I CTNAG 7 cut(s) 512, 878, 1180, 1193, 1523, 1572, 1610
HpySE526I ACGT 2 cut(s) 817, 1602
Hsp92II CATG 8 cut(s) 11, 292, 593, 704, 827, 915, 1159, 1463
HspAI GCGC 1 cut(s) 1288
Kzo9I GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
LmnI GCTCC 4 cut(s) 219, 508, 1009, 1049
Lsp1109I GCAGC 6 cut(s) 172, 387, 776, 855, 1076, 1454
LweI GCATC 5 cut(s) 758, 990, 1097, 1507, 1578
MabI ACCWGGT 1 cut(s) 1313
MaeI CTAG 2 cut(s) 87, 201
MaeII ACGT 2 cut(s) 817, 1602
MaeIII GTNAC 3 cut(s) 89, 694, 782
MalI GATC 6 cut(s) 414, 450, 564, 657, 1540, 1646
MboI GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
MboII GAAGA 6 cut(s) 66, 143, 347, 650, 929, 1174
MfeI CAATTG 3 cut(s) 492, 987, 1227
MflI RGATCY 2 cut(s) 448, 655
MhlI GDGCHC 4 cut(s) 179, 216, 1014, 1269
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 9 cut(s) 16, 26, 43, 70, 98, 492, 987, 1035, 1227
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 4 cut(s) 302, 476, 691, 798
MmeI TCCRAC 1 cut(s) 149
Mox20I TGGCCA 1 cut(s) 281
MscI TGGCCA 1 cut(s) 281
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 4 cut(s) 287, 705, 1272, 1458
Msp20I TGGCCA 1 cut(s) 281
MspA1I CMGCKG 4 cut(s) 767, 845, 868, 1184
MspI CCGG 3 cut(s) 506, 1256, 1501
MspR9I CCNGG 5 cut(s) 1256, 1315, 1340, 1502, 1560
MunI CAATTG 3 cut(s) 492, 987, 1227
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 3 cut(s) 1315, 1340, 1560
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 4 cut(s) 389, 874, 1094, 1440
NciI CCSGG 2 cut(s) 1256, 1502
NcoI CCATGG 1 cut(s) 1459
NdeI CATATG 1 cut(s) 1448
NdeII GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
NlaIII CATG 8 cut(s) 11, 292, 593, 704, 827, 915, 1159, 1463
NlaIV GGNNCC 5 cut(s) 176, 450, 601, 850, 1377
NmuCI GTSAC 2 cut(s) 694, 782
NspI RCATGY 2 cut(s) 11, 593
OliI CACNNNNGTG 1 cut(s) 1272
PaeI GCATGC 1 cut(s) 11
PaqCI CACCTGC 1 cut(s) 1102
PceI AGGCCT 1 cut(s) 898
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 5 cut(s) 204, 718, 919, 1043, 1236
Pfl23II CGTACG 1 cut(s) 471
PflFI GACNNNGTC 2 cut(s) 788, 1315
PflMI CCANNNNNTGG 1 cut(s) 977
PkrI GCNGC 9 cut(s) 187, 362, 377, 652, 766, 870, 1066, 1433, 1469
PleI GAGTC 4 cut(s) 302, 475, 691, 797
PpsI GAGTC 4 cut(s) 302, 475, 691, 797
Ppu21I YACGTR 1 cut(s) 818
PpuMI RGGWCCY 1 cut(s) 1376
Psp5II RGGWCCY 1 cut(s) 1376
Psp6I CCWGG 3 cut(s) 1313, 1338, 1558
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 3 cut(s) 1313, 1338, 1558
PspLI CGTACG 1 cut(s) 471
PspN4I GGNNCC 5 cut(s) 176, 450, 601, 850, 1377
PspPI GGNCC 4 cut(s) 600, 890, 1172, 1376
PspPPI RGGWCCY 1 cut(s) 1376
PstI CTGCAG 1 cut(s) 766
PsuI RGATCY 2 cut(s) 448, 655
PsyI GACNNNGTC 2 cut(s) 788, 1315
PvuII CAGCTG 3 cut(s) 845, 868, 1184
RsaI GTAC 3 cut(s) 473, 534, 1513
RsaNI GTAC 3 cut(s) 472, 533, 1512
RseI CAYNNNNRTG 4 cut(s) 287, 705, 1272, 1458
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 9 cut(s) 186, 361, 376, 651, 765, 869, 1065, 1432, 1468
Sau3AI GATC 6 cut(s) 412, 448, 562, 655, 1538, 1644
Sau96I GGNCC 4 cut(s) 600, 890, 1172, 1376
SchI GAGTC 4 cut(s) 302, 476, 691, 798
ScrFI CCNGG 5 cut(s) 1256, 1315, 1340, 1502, 1560
SduI GDGCHC 4 cut(s) 179, 216, 1014, 1269
SexAI ACCWGGT 1 cut(s) 1313
SfaNI GCATC 5 cut(s) 758, 990, 1097, 1507, 1578
SfcI CTRYAG 3 cut(s) 400, 762, 1389
SinI GGWCC 2 cut(s) 600, 1376
SmiMI CAYNNNNRTG 4 cut(s) 287, 705, 1272, 1458
SmlI CTYRAG 3 cut(s) 318, 662, 1307
SmoI CTYRAG 3 cut(s) 318, 662, 1307
SphI GCATGC 1 cut(s) 11
Sse9I AATT 9 cut(s) 16, 26, 43, 70, 98, 492, 987, 1035, 1227
SseBI AGGCCT 1 cut(s) 898
SsiI CCGC 6 cut(s) 361, 593, 651, 767, 1125, 1432
SspMI CTAG 2 cut(s) 87, 201
StuI AGGCCT 1 cut(s) 898
StyD4I CCNGG 5 cut(s) 1254, 1313, 1338, 1500, 1558
StyI CCWWGG 3 cut(s) 180, 893, 1459
TaaI ACNGT 3 cut(s) 647, 1144, 1438
TaiI ACGT 2 cut(s) 820, 1605
TaqI TCGA 9 cut(s) 222, 250, 441, 579, 617, 1234, 1400, 1533, 1647
TaqII GACCGA 1 cut(s) 1641
TasI AATT 9 cut(s) 16, 26, 43, 70, 98, 492, 987, 1035, 1227
TauI GCSGC 3 cut(s) 363, 653, 1434
TfiI GAWTC 5 cut(s) 204, 718, 919, 1043, 1236
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TseFI GTSAC 2 cut(s) 694, 782
TseI GCWGC 6 cut(s) 185, 375, 764, 868, 1064, 1467
Tsp45I GTSAC 2 cut(s) 694, 782
TspDTI ATGAA 2 cut(s) 1099, 1634
Tth111I GACNNNGTC 2 cut(s) 788, 1315
Van91I CCANNNNNTGG 1 cut(s) 977
VneI GTGCAC 1 cut(s) 1265
VpaK11BI GGWCC 2 cut(s) 600, 1376
XapI RAATTY 3 cut(s) 26, 43, 98
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 2 cut(s) 11, 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 1346
XmiI GTMKAC 1 cut(s) 1392
XspI CTAG 2 cut(s) 87, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.