Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0015497

Physical Location & Seq

Genomic Coordinates Reverse (-)

33617988 .. 33621942

Gene Structure 3955 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g43440.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGCTGCATCTTACTCAGTGCCTTCGATGATAATGGAGGAAGAAGGAAGGTTCGAAGCGGAGGTTGCGGAGGTGCAGGCATGGTGGGGATCCGAGAGGTTCAGGCTAACAAAACGACCCTACTCAGCCAAAGACGTTGTGGCACTCCGAGGCAGCCTCAGGCAGAGCTATGGCTCTAACGAAATGGCGAAAAAGCTCTGGAGAACTCTCAAGACTCACCAAGCCAACGGCACATCCTCAAGAACCTTCGGTGCTTTGGACCCTGTCCAAGTCACCATGATGGCCAAGCACTTGGACACAATCTATGTCTCCGGTTGGCAGTGCTCGTCGACACACACCAGCACCAACGAGCCGGGTCCTGACCTAGCTGACTACCCCTACGACACTGTCCCAAACAAGGTGGAGCACTTGTTCTTTGCTCAACAATACCATGACCGTAAGCAAAAGGAGGCGAGGATGAGCATGAGCAGGGAAGAGAGGGCTAGAACTCCTTATGTTGATTACTTGAAGCCCATCATTGCTGACGGGGACACCGGCTTTGGTGGCACCACCGCCACGGTGAAGCTCTGCAAGCTCTTTGTGGAGCGTGGCGCTGCTGGTGTTCACATTGAGGACCAGTCCTCCGTGACCAAAAAATGTGGTCACATGGCTGGCAAAGTGCTTGTTGCTGTGAGTGAGCACATCAACAGGCTCGTCTCGGCGAGGCTGCAGTTTGATGTGATGGGAACCGAGACGGTTTTGGTGGCCAGAACTGACGCCGTGGGAGCTACCTTGATCCAGACCAATGTGGACACTAGGGATCATCAGTTCATTTTGGGGGCAACGAATCCTAGTGTCAAGGGGAGAAGTTTGGCTACTCTTTTGGCTGAAGCAATGGCTGCTGGGAAAACAGGAGCTGAGCTTCAAGCACTTGAGGATGGCTGGATCTCAATGGCACAGCTGAAGACATTTAATCAATGTGTGACGGATGCGATCAATGCAACGAACATTGGTGAACATGAGAAGAGAAGGAGATTAAGCGAATGGGCGAACGATGCTAGTCATGAGAAGTGTTTGTCCAACGAGCAAGGCCGCCAGATTGCTGAGAGGTTGGGGCTTAATAACTTGTTCTGGGACTGGGACTTGCCTAGAACCAGAGAAGGGTTTTACAGGTTCCAAGGGTCCGTGGAGGCTGCAGTTGTCCGAGGATGGGCTTTTGCCAACCATGCTGATATTATCTGGATGGAGACGGCCAGCCCCGACATGGTTGAGTGCACGAAATTCGCACAAGGAGTAAAGACCATGAAACCAGAGATAATGCTGGCCTATAACCTCTCGCCCTCTTTCAATTGGGATGCTTCTGGAATGACTGATGAACAGATGAGGGACTTCATTCCGAGGATAGCCAAGTTGGGGTACTGCTGGCAGTTCATAACACTGGCTGGTTTCCATGCTGATGCACTAGTGATAGATACTTTTGCAAGGGACTACGCGAAGAGGGGAATGCTGGCTTATGTGGAGAGGATTCAGAGGGAGGAGAGGAACAATGGAGTCGACACGCTTGCGCATCAGAAATGGTCTGGTGCTAATTTCTATGATAGGTACCTCAAGACTGTCCAGGGAGGCATTTCTTCAACTGCTGCTATGGGAAAAGGAGTGACTGAAGAGCAATTTAAGGAATCATGGACTAGGCCAGGAGCTACTGATCTGAGTGAAGGCAATGTTGTGATGGCAAAGGCTAGAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016829 GO:0016830 GO:0016833 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00630 ko01100 ko01110 ko01120 ko01200 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

ICL

Protein Analysis

576

Amino Acids

64.28

Weight (kDa)

6.72

Isoelectric Point (pI)

31.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ICL	PF00463	21 - 551	1.6e-271	Isocitrate lyase family
PEP_mutase	PF13714	170 - 255	1.1e-09	Phosphoenolpyruvate phosphomutase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015497)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21720
fragaria_vesca	FvH4_6g43440
malus_domestica	MD09G1106500.v1.1 MD17G1094300.v1.1
prunus_persica	Prupe.3G219100_v2.0.a1
pyrus_communis	pycom17g09100
rosa_chinensis	RchiOBHm_Chr2g0159821
rosa_laevigata	RLG00000021183
rosa_multiflora	Rmu_sc0016393.1_g000006
rosa_roxburghii	Rroxscaffold_2G00090220
rosa_rugosa	Rorug02G0478900
rosa_samantha	Rh2AG545300 Rh2BG558500 Rh2CG528500 Rh2DG567600
rosa_wichuraiana	Rw2G045100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1545
Acc65I	GGTACC	1 cut(s)	1581
AccB1I	GGYRCC	2 cut(s)	545, 1581
AccI	GTMKAC	2 cut(s)	329, 1533
AccII	CGCG	1 cut(s)	1472
AciI	CCGC	4 cut(s)	59, 68, 552, 1072
AclWI	GGATC	5 cut(s)	84, 97, 769, 807, 932
AcoI	YGGCCR	3 cut(s)	282, 744, 1230
AcsI	RAATTY	1 cut(s)	1259
AcuI	CTGAAG	3 cut(s)	888, 962, 1662
AcyI	GRCGYC	1 cut(s)	756
AfaI	GTAC	2 cut(s)	1397, 1583
AfiI	CCNNNNNNNGG	5 cut(s)	397, 1140, 1189, 1243, 1392
AgsI	TTSAA	4 cut(s)	508, 905, 1327, 1614
AhlI	ACTAGT	1 cut(s)	1441
AjnI	CCWGG	2 cut(s)	1596, 1672
AloI	GAACNNNNNNTCC	4 cut(s)	395, 427, 791, 823
Alw21I	GWGCWC	4 cut(s)	326, 408, 681, 1256
Alw26I	GTCTC	4 cut(s)	313, 700, 725, 1220
Alw44I	GTGCAC	1 cut(s)	1252
AlwI	GGATC	5 cut(s)	84, 97, 769, 807, 932
AlwNI	CAGNNNCTG	1 cut(s)	896
AoxI	GGCC	6 cut(s)	282, 744, 1069, 1230, 1302, 1670
ApaLI	GTGCAC	1 cut(s)	1252
ApeKI	GCWGC	7 cut(s)	5, 153, 593, 706, 878, 1172, 1619
ApoI	RAATTY	1 cut(s)	1259
ArsI	GACNNNNNNTTYG	2 cut(s)	521, 553
Asp718I	GGTACC	1 cut(s)	1581
AspLEI	GCGC	2 cut(s)	593, 1546
AspS9I	GGNCC	4 cut(s)	259, 356, 613, 1161
AsuC2I	CCSGG	1 cut(s)	354
AsuHPI	GGTGA	4 cut(s)	209, 265, 571, 1004
AsuII	TTCGAA	1 cut(s)	54
AvaII	GGWCC	4 cut(s)	259, 356, 613, 1161
AxyI	CCTNAGG	1 cut(s)	158
BaeGI	GKGCMC	1 cut(s)	1256
BalI	TGGCCA	2 cut(s)	284, 746
BamHI	GGATCC	1 cut(s)	89
BanI	GGYRCC	2 cut(s)	545, 1581
BarI	GAAGNNNNNNTAC	2 cut(s)	838, 870
BbsI	GAAGAC	1 cut(s)	950
Bbv12I	GWGCWC	4 cut(s)	326, 408, 681, 1256
BbvI	GCAGC	6 cut(s)	165, 580, 693, 865, 1159, 1606
BccI	CCATC	7 cut(s)	274, 521, 715, 911, 1182, 1216, 1702
BceAI	ACGGC	3 cut(s)	244, 743, 1245
BcgI	CGANNNNNNTGC	4 cut(s)	1243, 1277, 1523, 1557
BciT130I	CCWGG	2 cut(s)	1598, 1674
BcnI	CCSGG	1 cut(s)	354
BcoDI	GTCTC	4 cut(s)	313, 700, 725, 1220
BcuI	ACTAGT	1 cut(s)	1441
BfaI	CTAG	9 cut(s)	365, 483, 795, 831, 1038, 1128, 1442, 1668, 1719
BfmI	CTRYAG	2 cut(s)	707, 1173
BfoI	RGCGCY	1 cut(s)	594
BisI	GCNGC	8 cut(s)	6, 154, 594, 707, 879, 1072, 1173, 1620
BlpI	GCTNAGC	1 cut(s)	897
BlsI	GCNGC	8 cut(s)	7, 155, 595, 708, 880, 1073, 1174, 1621
Bme1390I	CCNGG	3 cut(s)	354, 1598, 1674
Bme18I	GGWCC	4 cut(s)	259, 356, 613, 1161
BmgT120I	GGNCC	4 cut(s)	259, 356, 613, 1161
BmiI	GGNNCC	8 cut(s)	91, 261, 357, 547, 727, 1154, 1162, 1583
BmrFI	CCNGG	3 cut(s)	354, 1598, 1674
BmrI	ACTGGG	1 cut(s)	1126
BmsI	GCATC	6 cut(s)	17, 958, 1024, 1324, 1426, 1555
BmuI	ACTGGG	1 cut(s)	1126
BpiI	GAAGAC	1 cut(s)	950
BplI	GAGNNNNNCTC	2 cut(s)	141, 173
BpmI	CTGGAG	1 cut(s)	220
Bpu1102I	GCTNAGC	1 cut(s)	897
Bpu14I	TTCGAA	1 cut(s)	54
BpuEI	CTTGAG	4 cut(s)	194, 223, 932, 1571
BpuMI	CCSGG	1 cut(s)	354
BsaHI	GRCGYC	1 cut(s)	756
BsaJI	CCNNGG	8 cut(s)	148, 555, 759, 1156, 1164, 1183, 1376, 1597
BsaWI	WCCGGW	1 cut(s)	311
BsaXI	ACNNNNNCTCC	4 cut(s)	395, 425, 605, 635
Bsc4I	CCNNNNNNNGG	5 cut(s)	397, 1140, 1189, 1243, 1392
Bse118I	RCCGGY	1 cut(s)	533
Bse1I	ACTGG	3 cut(s)	616, 1121, 1422
Bse21I	CCTNAGG	1 cut(s)	158
Bse3DI	GCAATG	3 cut(s)	516, 879, 1705
BseBI	CCWGG	2 cut(s)	1598, 1674
BseDI	CCNNGG	8 cut(s)	148, 555, 759, 1156, 1164, 1183, 1376, 1597
BseGI	GGATG	7 cut(s)	233, 462, 922, 973, 1193, 1227, 1339
BseLI	CCNNNNNNNGG	5 cut(s)	397, 1140, 1189, 1243, 1392
BseMI	GCAATG	3 cut(s)	516, 879, 1705
BseMII	CTCAG	6 cut(s)	30, 138, 172, 888, 1074, 1679
BseNI	ACTGG	3 cut(s)	616, 1121, 1422
BseRI	GAGGAG	1 cut(s)	1529
BseSI	GKGCMC	1 cut(s)	1256
BseXI	GCAGC	6 cut(s)	165, 580, 693, 865, 1159, 1606
BseYI	CCCAGC	1 cut(s)	881
BsgI	GTGCAG	1 cut(s)	95
Bsh1236I	CGCG	1 cut(s)	1472
BshFI	GGCC	6 cut(s)	284, 746, 1071, 1232, 1304, 1672
BshNI	GGYRCC	2 cut(s)	545, 1581
BsiHKAI	GWGCWC	4 cut(s)	326, 408, 681, 1256
BsiSI	CCGG	3 cut(s)	312, 353, 534
BslFI	GGGAC	6 cut(s)	374, 542, 1127, 1133, 1379, 1478
BslI	CCNNNNNNNGG	5 cut(s)	397, 1140, 1189, 1243, 1392
BsmAI	GTCTC	4 cut(s)	313, 700, 725, 1220
BsmBI	CGTCTC	3 cut(s)	700, 725, 1220
BsmFI	GGGAC	6 cut(s)	374, 542, 1127, 1133, 1379, 1478
BsmI	GAATGC	1 cut(s)	1488
BsnI	GGCC	6 cut(s)	284, 746, 1071, 1232, 1304, 1672
Bsp119I	TTCGAA	1 cut(s)	54
Bsp1286I	GDGCHC	4 cut(s)	326, 408, 681, 1256
Bsp143I	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
Bsp1720I	GCTNAGC	1 cut(s)	897
BspACI	CCGC	4 cut(s)	59, 68, 552, 1072
BspANI	GGCC	6 cut(s)	284, 746, 1071, 1232, 1304, 1672
BspCNI	CTCAG	6 cut(s)	29, 137, 171, 889, 1075, 1680
BspFNI	CGCG	1 cut(s)	1472
BspHI	TCATGA	1 cut(s)	1042
BspLI	GGNNCC	8 cut(s)	91, 261, 357, 547, 727, 1154, 1162, 1583
BspMAI	CTGCAG	2 cut(s)	711, 1177
BspPI	GGATC	5 cut(s)	84, 97, 769, 807, 932
BspQI	GCTCTTC	1 cut(s)	1638
BspT104I	TTCGAA	1 cut(s)	54
BspT107I	GGYRCC	2 cut(s)	545, 1581
BsrDI	GCAATG	3 cut(s)	516, 879, 1705
BsrFI	RCCGGY	1 cut(s)	533
BsrI	ACTGG	3 cut(s)	616, 1121, 1422
BssAI	RCCGGY	1 cut(s)	533
BssECI	CCNNGG	8 cut(s)	148, 555, 759, 1156, 1164, 1183, 1376, 1597
BssMI	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
BssNI	GRCGYC	1 cut(s)	756
BssT1I	CCWWGG	1 cut(s)	1156
Bst2UI	CCWGG	2 cut(s)	1598, 1674
Bst4CI	ACNGT	5 cut(s)	388, 437, 559, 736, 1594
Bst6I	CTCTTC	4 cut(s)	468, 998, 1469, 1638
BstACI	GRCGYC	1 cut(s)	756
BstAPI	GCANNNNNTGC	1 cut(s)	878
BstBI	TTCGAA	1 cut(s)	54
BstC8I	GCNNGC	8 cut(s)	78, 572, 652, 1234, 1302, 1403, 1488, 1542
BstDEI	CTNAG	6 cut(s)	16, 124, 158, 897, 1083, 1688
BstDSI	CCRYGG	3 cut(s)	555, 759, 1164
BstF5I	GGATG	7 cut(s)	233, 462, 922, 973, 1193, 1227, 1339
BstFNI	CGCG	1 cut(s)	1472
BstH2I	RGCGCY	1 cut(s)	594
BstHHI	GCGC	2 cut(s)	593, 1546
BstKTI	GATC	6 cut(s)	92, 777, 802, 927, 975, 1687
BstMAI	GTCTC	4 cut(s)	313, 700, 725, 1220
BstMBI	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
BstMWI	GCNNNNNNNGC	8 cut(s)	65, 543, 571, 764, 878, 977, 1034, 1205
BstNI	CCWGG	2 cut(s)	1598, 1674
BstSCI	CCNGG	3 cut(s)	352, 1596, 1672
BstSFI	CTRYAG	2 cut(s)	707, 1173
BstSLI	GKGCMC	1 cut(s)	1256
BstUI	CGCG	1 cut(s)	1472
BstV1I	GCAGC	6 cut(s)	165, 580, 693, 865, 1159, 1606
BstV2I	GAAGAC	1 cut(s)	950
BstX2I	RGATCY	2 cut(s)	89, 924
BstXI	CCANNNNNNTGG	1 cut(s)	292
BstYI	RGATCY	2 cut(s)	89, 924
Bsu36I	CCTNAGG	1 cut(s)	158
BsuRI	GGCC	6 cut(s)	284, 746, 1071, 1232, 1304, 1672
BtgI	CCRYGG	3 cut(s)	555, 759, 1164
BtsCI	GGATG	7 cut(s)	233, 462, 922, 973, 1193, 1227, 1339
BtsI	GCAGTG	1 cut(s)	326
BtsIMutI	CAGTG	4 cut(s)	24, 326, 384, 1415
Cac8I	GCNNGC	8 cut(s)	78, 572, 652, 1234, 1302, 1403, 1488, 1542
CaiI	CAGNNNCTG	1 cut(s)	896
CciI	TCATGA	1 cut(s)	1042
CfoI	GCGC	2 cut(s)	593, 1546
Cfr10I	RCCGGY	1 cut(s)	533
Cfr13I	GGNCC	4 cut(s)	259, 356, 613, 1161
CseI	GACGC	1 cut(s)	764
Csp6I	GTAC	2 cut(s)	1396, 1582
CspCI	CAANNNNNGTGG	4 cut(s)	381, 416, 619, 654
CviQI	GTAC	2 cut(s)	1396, 1582
DdeI	CTNAG	6 cut(s)	16, 124, 158, 897, 1083, 1688
DpnI	GATC	6 cut(s)	91, 776, 801, 926, 974, 1686
DpnII	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
EaeI	YGGCCR	3 cut(s)	282, 744, 1230
Eam1104I	CTCTTC	4 cut(s)	468, 998, 1469, 1638
EarI	CTCTTC	4 cut(s)	468, 998, 1469, 1638
Eco130I	CCWWGG	1 cut(s)	1156
Eco47I	GGWCC	4 cut(s)	259, 356, 613, 1161
Eco57I	CTGAAG	3 cut(s)	888, 962, 1662
Eco81I	CCTNAGG	1 cut(s)	158
EcoO109I	RGGNCCY	1 cut(s)	356
EcoRII	CCWGG	2 cut(s)	1596, 1672
EcoT14I	CCWWGG	1 cut(s)	1156
ErhI	CCWWGG	1 cut(s)	1156
Esp3I	CGTCTC	3 cut(s)	700, 725, 1220
FaqI	GGGAC	6 cut(s)	374, 542, 1127, 1133, 1379, 1478
FblI	GTMKAC	2 cut(s)	329, 1533
Fnu4HI	GCNGC	8 cut(s)	6, 154, 594, 707, 879, 1072, 1173, 1620
FokI	GGATG	7 cut(s)	220, 469, 929, 980, 1200, 1234, 1346
Fsp4HI	GCNGC	8 cut(s)	6, 154, 594, 707, 879, 1072, 1173, 1620
FspBI	CTAG	9 cut(s)	365, 483, 795, 831, 1038, 1128, 1442, 1668, 1719
FspI	TGCGCA	1 cut(s)	1545
GlaI	GCGC	2 cut(s)	592, 1545
GluI	GCNGC	8 cut(s)	6, 154, 594, 707, 879, 1072, 1173, 1620
GsaI	CCCAGC	1 cut(s)	885
GsuI	CTGGAG	1 cut(s)	220
HaeII	RGCGCY	1 cut(s)	594
HaeIII	GGCC	6 cut(s)	284, 746, 1071, 1232, 1304, 1672
HapII	CCGG	3 cut(s)	312, 353, 534
HgaI	GACGC	1 cut(s)	764
HhaI	GCGC	2 cut(s)	593, 1546
Hin1I	GRCGYC	1 cut(s)	756
Hin6I	GCGC	2 cut(s)	591, 1544
HinP1I	GCGC	2 cut(s)	591, 1544
HincII	GTYRAC	2 cut(s)	330, 1534
HindII	GTYRAC	2 cut(s)	330, 1534
HinfI	GANTC	5 cut(s)	214, 826, 1504, 1530, 1658
HpaII	CCGG	3 cut(s)	312, 353, 534
HphI	GGTGA	4 cut(s)	209, 265, 571, 1004
Hpy166II	GTNNAC	6 cut(s)	330, 604, 790, 995, 1254, 1534
Hpy188I	TCNGA	7 cut(s)	94, 149, 1184, 1377, 1509, 1551, 1689
Hpy188III	TCNNGA	9 cut(s)	199, 211, 240, 359, 778, 1043, 1219, 1341, 1588
Hpy8I	GTNNAC	6 cut(s)	330, 604, 790, 995, 1254, 1534
Hpy99I	CGWCG	1 cut(s)	331
HpyAV	CCTTC	7 cut(s)	33, 38, 42, 256, 1002, 1133, 1688
HpyCH4III	ACNGT	5 cut(s)	388, 437, 559, 736, 1594
HpyCH4IV	ACGT	1 cut(s)	135
HpyCH4V	TGCA	9 cut(s)	8, 76, 570, 709, 980, 1175, 1254, 1439, 1460
HpyF10VI	GCNNNNNNNGC	8 cut(s)	65, 543, 571, 764, 878, 977, 1034, 1205
HpyF3I	CTNAG	6 cut(s)	16, 124, 158, 897, 1083, 1688
HpySE526I	ACGT	1 cut(s)	135
Hsp92I	GRCGYC	1 cut(s)	756
HspAI	GCGC	2 cut(s)	591, 1544
KpnI	GGTACC	1 cut(s)	1585
Kzo9I	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
LguI	GCTCTTC	1 cut(s)	1638
LmnI	GCTCC	5 cut(s)	403, 583, 764, 893, 1676
Lsp1109I	GCAGC	6 cut(s)	165, 580, 693, 865, 1159, 1606
LweI	GCATC	6 cut(s)	17, 958, 1024, 1324, 1426, 1555
MaeI	CTAG	9 cut(s)	365, 483, 795, 831, 1038, 1128, 1442, 1668, 1719
MaeII	ACGT	1 cut(s)	135
MaeIII	GTNAC	5 cut(s)	271, 625, 641, 961, 1636
MalI	GATC	6 cut(s)	91, 776, 801, 926, 974, 1686
MboI	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
MboII	GAAGA	7 cut(s)	53, 485, 955, 1015, 1486, 1602, 1655
MfeI	CAATTG	1 cut(s)	1327
MflI	RGATCY	2 cut(s)	89, 924
MhlI	GDGCHC	4 cut(s)	326, 408, 681, 1256
MlsI	TGGCCA	2 cut(s)	284, 746
MluCI	AATT	4 cut(s)	1259, 1327, 1567, 1649
MluNI	TGGCCA	2 cut(s)	284, 746
MlyI	GAGTC	2 cut(s)	208, 1539
MmeI	TCCRAC	1 cut(s)	1083
Mox20I	TGGCCA	2 cut(s)	284, 746
MscI	TGGCCA	2 cut(s)	284, 746
MseI	TTAA	4 cut(s)	951, 1016, 1098, 1653
MslI	CAYNNNNRTG	2 cut(s)	278, 1434
Msp20I	TGGCCA	2 cut(s)	284, 746
MspA1I	CMGCKG	1 cut(s)	940
MspI	CCGG	3 cut(s)	312, 353, 534
MspR9I	CCNGG	3 cut(s)	354, 1598, 1674
MunI	CAATTG	1 cut(s)	1327
Mva1269I	GAATGC	1 cut(s)	1488
MvaI	CCWGG	2 cut(s)	1598, 1674
MvnI	CGCG	1 cut(s)	1472
MwoI	GCNNNNNNNGC	8 cut(s)	65, 543, 571, 764, 878, 977, 1034, 1205
NciI	CCSGG	1 cut(s)	354
NdeII	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
NlaIV	GGNNCC	8 cut(s)	91, 261, 357, 547, 727, 1154, 1162, 1583
NmeAIII	GCCGAG	1 cut(s)	677
NmuCI	GTSAC	5 cut(s)	271, 625, 641, 961, 1636
NsbI	TGCGCA	1 cut(s)	1545
NspV	TTCGAA	1 cut(s)	54
PagI	TCATGA	1 cut(s)	1042
PciSI	GCTCTTC	1 cut(s)	1638
PctI	GAATGC	1 cut(s)	1488
PfeI	GAWTC	3 cut(s)	826, 1504, 1658
PflFI	GACNNNGTC	2 cut(s)	263, 386
PkrI	GCNGC	8 cut(s)	7, 155, 595, 708, 880, 1073, 1174, 1621
PleI	GAGTC	2 cut(s)	208, 1538
PpsI	GAGTC	2 cut(s)	208, 1538
PpuMI	RGGWCCY	1 cut(s)	356
Psp5II	RGGWCCY	1 cut(s)	356
Psp6I	CCWGG	2 cut(s)	1596, 1672
PspFI	CCCAGC	1 cut(s)	881
PspGI	CCWGG	2 cut(s)	1596, 1672
PspN4I	GGNNCC	8 cut(s)	91, 261, 357, 547, 727, 1154, 1162, 1583
PspPI	GGNCC	4 cut(s)	259, 356, 613, 1161
PspPPI	RGGWCCY	1 cut(s)	356
PstI	CTGCAG	2 cut(s)	711, 1177
PstNI	CAGNNNCTG	1 cut(s)	896
PsuI	RGATCY	2 cut(s)	89, 924
PsyI	GACNNNGTC	2 cut(s)	263, 386
PvuII	CAGCTG	1 cut(s)	940
RsaI	GTAC	2 cut(s)	1397, 1583
RsaNI	GTAC	2 cut(s)	1396, 1582
RseI	CAYNNNNRTG	2 cut(s)	278, 1434
SalI	GTCGAC	2 cut(s)	328, 1532
SapI	GCTCTTC	1 cut(s)	1638
SaqAI	TTAA	4 cut(s)	951, 1016, 1098, 1653
SatI	GCNGC	8 cut(s)	6, 154, 594, 707, 879, 1072, 1173, 1620
Sau3AI	GATC	6 cut(s)	89, 774, 799, 924, 972, 1684
Sau96I	GGNCC	4 cut(s)	259, 356, 613, 1161
SchI	GAGTC	2 cut(s)	208, 1539
ScrFI	CCNGG	3 cut(s)	354, 1598, 1674
SduI	GDGCHC	4 cut(s)	326, 408, 681, 1256
SfaNI	GCATC	6 cut(s)	17, 958, 1024, 1324, 1426, 1555
SfcI	CTRYAG	2 cut(s)	707, 1173
SfuI	TTCGAA	1 cut(s)	54
SinI	GGWCC	4 cut(s)	259, 356, 613, 1161
SmiMI	CAYNNNNRTG	2 cut(s)	278, 1434
SmlI	CTYRAG	4 cut(s)	209, 238, 911, 1586
SmoI	CTYRAG	4 cut(s)	209, 238, 911, 1586
SpeI	ACTAGT	1 cut(s)	1441
Sse9I	AATT	4 cut(s)	1259, 1327, 1567, 1649
SsiI	CCGC	4 cut(s)	59, 68, 552, 1072
SspMI	CTAG	9 cut(s)	365, 483, 795, 831, 1038, 1128, 1442, 1668, 1719
StyD4I	CCNGG	3 cut(s)	352, 1596, 1672
StyI	CCWWGG	1 cut(s)	1156
TaaI	ACNGT	5 cut(s)	388, 437, 559, 736, 1594
TaiI	ACGT	1 cut(s)	138
TaqI	TCGA	4 cut(s)	26, 54, 329, 1533
TasI	AATT	4 cut(s)	1259, 1327, 1567, 1649
TauI	GCSGC	1 cut(s)	1074
TfiI	GAWTC	3 cut(s)	826, 1504, 1658
Tru1I	TTAA	4 cut(s)	951, 1016, 1098, 1653
Tru9I	TTAA	4 cut(s)	951, 1016, 1098, 1653
TscAI	CASTG	4 cut(s)	24, 326, 391, 1422
TseFI	GTSAC	5 cut(s)	271, 625, 641, 961, 1636
TseI	GCWGC	7 cut(s)	5, 153, 593, 706, 878, 1172, 1619
Tsp45I	GTSAC	5 cut(s)	271, 625, 641, 961, 1636
TspDTI	ATGAA	5 cut(s)	799, 1298, 1360, 1368, 1399
TspGWI	ACGGA	3 cut(s)	613, 980, 1153
TspRI	CASTG	4 cut(s)	24, 326, 391, 1422
Tth111I	GACNNNGTC	2 cut(s)	263, 386
VneI	GTGCAC	1 cut(s)	1252
VpaK11BI	GGWCC	4 cut(s)	259, 356, 613, 1161
XapI	RAATTY	1 cut(s)	1259
XcmI	CCANNNNNNNNNTGG	2 cut(s)	136, 1240
XmiI	GTMKAC	2 cut(s)	329, 1533
XspI	CTAG	9 cut(s)	365, 483, 795, 831, 1038, 1128, 1442, 1668, 1719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.