Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0015497

Physical Location & Seq

Genomic Coordinates Forward (+)

7930647 .. 7933067

Gene Structure 2421 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1094300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGCTGCATCTTACTCAGTGCCTTCAATGATAATGGAAGAAGAAGGAAGGTTTGAGGCAGAAGTCGCGGAGGTGCATGCATGGTGGGGCTCAGAAAGGTTCAAGCTCACAAAACGACCATACTCAGCTAGAGACGTTGTGGCGCTAAGAGGCACCCTAAGACAAAGCTATGGCTCAAATGAAATGGCCAAAAAGCTGTGGAGAACTCTCAAAATTCACCAAGCCAATGGCACATCCTCAAGGACTTTTGGTGCCCTAGACCCCGTCCAGGTCACCATGATGGCCAAGCACTTGGACACAATTTACGTCTCTGGTTGGCAATGTTCGTCCACACACACCAGCACCAATGAGCCTGGTCCCGACCTTGCTGACTACCCTTATGACACGGTCCCAAACAAGGTGGAGCATTTGTTTTTCGCTCAACAATACCATGACCGCAAGCAAAAGGAGGCAAGGATGAGCATGAGCCGGGAGGAGAGGGCTAGAACCCCTTATGTTGATTACTTGAAGCCAATCGTTGCTGATGGTGACACTGGTTTTGGTGGCACCACGGCGACTGTTAAGCTTTGCAAGCTTTTCGTGGAGCGAGGCGCTGCTGGTGTTCACATTGAGGACCAGTCATCTGTGACAAAAAAATGTGGTCACATGGCTGGGAAAGTGCTTGTTGCTGTTAGTGAACATATTAACAGACTTGTGGCTGCAAGGCTACAATTTGATGTTATGGGAACTGAGACTGTATTGGTGGCTAGAACCGATGCGGTTGCAGCTACATTGATCCAGACCAATGTGGACACTAGGGATCATCAGTTTATATTGGGGGCTTCGAACCAAAATCTTAGAGGGAAGAGCTTGGCTACCCTTTTGGCTGAAGCAATGGCCGCTGGCAAAACAGGAGCTGAGCTGCAGGCCCTCGAGGACAACTGGACTTCAATGGCACAGCTGAAGACATTTTCAGAATGTGTCACTGATGCAATCAAGGCCATGAACCTTGGCGAACATGAAAAAAGGAGGAGATTGAGTGAATGGATGAGCTATTCTAGTCCTAAAAAGTGTTTGTCAAATGAGCAAGGGCGCGAAATCGCTGAGAAGTTGGGGCTTAAGAACCTTTTCTGGGACTGGGACTTGCCTAGAACAAGAGAAGGGTTCTACAGGTTCAAAGGGTCTGTGATGGCAGCTGTCGTGCGTGGATGGGCTTTTGCTGACCACGCTGACATTATTTGGATGGAGACTTCGAGCCCCAATATGGTTGAGTGCACCCAATTTGCTGAGGGGGTGAAGAATAAGCAGCCTGAAATCATGTTAGCTTATAATCTTTCACCATCTTTCAACTGGGATGCATCTGGGATGAATGATCAGCAAATGATGGACTTTATTCCTAGGATTGCCAAGCTTGGCTACTGCTGGCAATTTATTACACTGGCCGGTTTCCATGCAGATGCGCTTGTGGTTGATACTTTTGCGAGGGACTATGCGAGGAGGGGAATGCTGGCTTATGTTGAAAGGATCCAGAGGGAGGAGAGGAACAATGGAGTTGACACACTTGCTCATCAGAAGTGGTCTGGTGCTAATTTCTATGATAGGTACCTCAAGACTCAAAATTCTGTATGGAAAAAAAGTTCACGTGTTCAAGAAGAAGGCGATCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016829 GO:0016830 GO:0016833 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00630 ko01100 ko01110 ko01120 ko01200 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

ICL

Protein Analysis

549

Amino Acids

61.85

Weight (kDa)

7.64

Isoelectric Point (pI)

35.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ICL	PF00463	21 - 532	5.3e-259	Isocitrate lyase family
PEP_mutase	PF13714	170 - 256	3.9e-10	Phosphoenolpyruvate phosphomutase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015497)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21720
fragaria_vesca	FvH4_6g43440
malus_domestica	MD09G1106500.v1.1 MD17G1094300.v1.1
prunus_persica	Prupe.3G219100_v2.0.a1
pyrus_communis	pycom17g09100
rosa_chinensis	RchiOBHm_Chr2g0159821
rosa_laevigata	RLG00000021183
rosa_multiflora	Rmu_sc0016393.1_g000006
rosa_roxburghii	Rroxscaffold_2G00090220
rosa_rugosa	Rorug02G0478900
rosa_samantha	Rh2AG545300 Rh2BG558500 Rh2CG528500 Rh2DG567600
rosa_wichuraiana	Rw2G045100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1308
AbsI	CCTCGAGG	1 cut(s)	911
Acc65I	GGTACC	1 cut(s)	1581
AccB1I	GGYRCC	4 cut(s)	152, 251, 545, 1581
AccII	CGCG	2 cut(s)	68, 1074
AciI	CCGC	4 cut(s)	68, 436, 758, 879
AclWI	GGATC	5 cut(s)	769, 807, 1498, 1511, 1634
AcoI	YGGCCR	4 cut(s)	186, 282, 876, 1419
AcsI	RAATTY	2 cut(s)	213, 1597
AcuI	CTGAAG	2 cut(s)	888, 962
AcvI	CACGTG	1 cut(s)	1622
AfaI	GTAC	1 cut(s)	1583
AfiI	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
AflII	CTTAAG	1 cut(s)	1097
AflIII	ACRYGT	1 cut(s)	1621
AgsI	TTSAA	8 cut(s)	27, 103, 508, 930, 1156, 1327, 1499, 1628
AjnI	CCWGG	2 cut(s)	267, 352
AloI	GAACNNNNNNTCC	2 cut(s)	1600, 1632
Alw21I	GWGCWC	1 cut(s)	1256
Alw26I	GTCTC	4 cut(s)	126, 313, 725, 1220
Alw44I	GTGCAC	1 cut(s)	1252
AlwI	GGATC	5 cut(s)	769, 807, 1498, 1511, 1634
AlwNI	CAGNNNCTG	1 cut(s)	896
Ama87I	CYCGRG	1 cut(s)	911
AoxI	GGCC	6 cut(s)	186, 282, 876, 906, 978, 1419
ApaLI	GTGCAC	1 cut(s)	1252
ApeKI	GCWGC	7 cut(s)	5, 593, 698, 764, 901, 1172, 1285
ApoI	RAATTY	2 cut(s)	213, 1597
ArsI	GACNNNNNNTTYG	2 cut(s)	521, 553
Asp700I	GAANNNNTTC	1 cut(s)	1106
Asp718I	GGTACC	1 cut(s)	1581
AspA2I	CCTAGG	1 cut(s)	1376
AspLEI	GCGC	4 cut(s)	145, 593, 1074, 1441
AspS9I	GGNCC	4 cut(s)	356, 388, 613, 907
AsuC2I	CCSGG	1 cut(s)	470
AsuHPI	GGTGA	5 cut(s)	209, 265, 539, 1285, 1308
AsuII	TTCGAA	1 cut(s)	824
AvaI	CYCGRG	1 cut(s)	911
AvaII	GGWCC	3 cut(s)	356, 388, 613
AvrII	CCTAGG	1 cut(s)	1376
BaeGI	GKGCMC	2 cut(s)	256, 1256
BalI	TGGCCA	2 cut(s)	188, 284
BamHI	GGATCC	1 cut(s)	1503
BanI	GGYRCC	4 cut(s)	152, 251, 545, 1581
BanII	GRGCYC	2 cut(s)	92, 1238
BbrPI	CACGTG	1 cut(s)	1622
BbsI	GAAGAC	1 cut(s)	950
Bbv12I	GWGCWC	1 cut(s)	1256
BbvCI	CCTCAGC	1 cut(s)	1266
BbvI	GCAGC	6 cut(s)	580, 685, 776, 888, 1184, 1297
BccI	CCATC	7 cut(s)	274, 518, 1162, 1182, 1216, 1327, 1357
BceAI	ACGGC	1 cut(s)	567
BcgI	CGANNNNNNTGC	4 cut(s)	559, 593, 743, 777
BciT130I	CCWGG	2 cut(s)	269, 354
BclI	TGATCA	1 cut(s)	1351
BcnI	CCSGG	1 cut(s)	470
BcoDI	GTCTC	4 cut(s)	126, 313, 725, 1220
BfaI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
BfmI	CTRYAG	2 cut(s)	902, 1147
BfoI	RGCGCY	2 cut(s)	146, 594
BfrI	CTTAAG	1 cut(s)	1097
BisI	GCNGC	8 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286
BlnI	CCTAGG	1 cut(s)	1376
BlpI	GCTNAGC	1 cut(s)	897
BlsI	GCNGC	8 cut(s)	7, 595, 700, 766, 880, 903, 1174, 1287
Bme1390I	CCNGG	3 cut(s)	269, 354, 470
Bme18I	GGWCC	3 cut(s)	356, 388, 613
BmeT110I	CYCGRG	1 cut(s)	911
BmgT120I	GGNCC	4 cut(s)	356, 388, 613, 907
BmiI	GGNNCC	7 cut(s)	154, 253, 358, 390, 547, 1505, 1583
BmrFI	CCNGG	3 cut(s)	269, 354, 470
BmrI	ACTGGG	2 cut(s)	1126, 1339
BmsI	GCATC	6 cut(s)	17, 745, 958, 1324, 1346, 1426
BmuI	ACTGGG	2 cut(s)	1126, 1339
BpiI	GAAGAC	1 cut(s)	950
Bpu10I	CCTNAGC	1 cut(s)	1266
Bpu1102I	GCTNAGC	1 cut(s)	897
Bpu14I	TTCGAA	1 cut(s)	824
BpuEI	CTTGAG	2 cut(s)	223, 1571
BpuMI	CCSGG	1 cut(s)	470
BsaAI	YACGTR	1 cut(s)	1622
BsaJI	CCNNGG	3 cut(s)	549, 988, 1376
BsaXI	ACNNNNNCTCC	4 cut(s)	395, 425, 1003, 1033
Bsc4I	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
Bse118I	RCCGGY	1 cut(s)	1421
Bse1I	ACTGG	6 cut(s)	538, 616, 926, 1121, 1334, 1422
Bse3DI	GCAATG	2 cut(s)	326, 879
BseBI	CCWGG	2 cut(s)	269, 354
BseDI	CCNNGG	3 cut(s)	549, 988, 1376
BseGI	GGATG	7 cut(s)	233, 462, 1032, 1193, 1227, 1339, 1350
BseLI	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
BseMI	GCAATG	2 cut(s)	326, 879
BseMII	CTCAG	7 cut(s)	30, 105, 138, 720, 888, 1074, 1257
BseNI	ACTGG	6 cut(s)	538, 616, 926, 1121, 1334, 1422
BseRI	GAGGAG	4 cut(s)	488, 1024, 1489, 1529
BseSI	GKGCMC	2 cut(s)	256, 1256
BseXI	GCAGC	6 cut(s)	580, 685, 776, 888, 1184, 1297
BseYI	CCCAGC	1 cut(s)	650
Bsh1236I	CGCG	2 cut(s)	68, 1074
BshFI	GGCC	6 cut(s)	188, 284, 878, 908, 980, 1421
BshNI	GGYRCC	4 cut(s)	152, 251, 545, 1581
BsiHKAI	GWGCWC	1 cut(s)	1256
BsiHKCI	CYCGRG	1 cut(s)	911
BsiSI	CCGG	2 cut(s)	469, 1422
BslFI	GGGAC	5 cut(s)	342, 374, 1127, 1133, 1478
BslI	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
BsmAI	GTCTC	4 cut(s)	126, 313, 725, 1220
BsmBI	CGTCTC	2 cut(s)	126, 313
BsmFI	GGGAC	5 cut(s)	342, 374, 1127, 1133, 1478
BsmI	GAATGC	1 cut(s)	1488
BsnI	GGCC	6 cut(s)	188, 284, 878, 908, 980, 1421
BsoBI	CYCGRG	1 cut(s)	911
Bsp119I	TTCGAA	1 cut(s)	824
Bsp1286I	GDGCHC	4 cut(s)	92, 256, 1238, 1256
Bsp143I	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
Bsp1720I	GCTNAGC	1 cut(s)	897
BspACI	CCGC	4 cut(s)	68, 436, 758, 879
BspANI	GGCC	6 cut(s)	188, 284, 878, 908, 980, 1421
BspCNI	CTCAG	7 cut(s)	29, 104, 137, 721, 889, 1075, 1258
BspFNI	CGCG	2 cut(s)	68, 1074
BspLI	GGNNCC	7 cut(s)	154, 253, 358, 390, 547, 1505, 1583
BspMAI	CTGCAG	1 cut(s)	906
BspPI	GGATC	5 cut(s)	769, 807, 1498, 1511, 1634
BspQI	GCTCTTC	1 cut(s)	839
BspT104I	TTCGAA	1 cut(s)	824
BspT107I	GGYRCC	4 cut(s)	152, 251, 545, 1581
BspTI	CTTAAG	1 cut(s)	1097
BsrDI	GCAATG	2 cut(s)	326, 879
BsrFI	RCCGGY	1 cut(s)	1421
BsrI	ACTGG	6 cut(s)	538, 616, 926, 1121, 1334, 1422
BssAI	RCCGGY	1 cut(s)	1421
BssECI	CCNNGG	3 cut(s)	549, 988, 1376
BssMI	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
BssT1I	CCWWGG	2 cut(s)	988, 1376
Bst2UI	CCWGG	2 cut(s)	269, 354
Bst4CI	ACNGT	3 cut(s)	388, 559, 736
Bst6I	CTCTTC	1 cut(s)	839
BstAFI	CTTAAG	1 cut(s)	1097
BstBAI	YACGTR	1 cut(s)	1622
BstBI	TTCGAA	1 cut(s)	824
BstC8I	GCNNGC	7 cut(s)	78, 440, 572, 883, 906, 1403, 1488
BstDSI	CCRYGG	1 cut(s)	549
BstEII	GGTNACC	1 cut(s)	271
BstF5I	GGATG	7 cut(s)	233, 462, 1032, 1193, 1227, 1339, 1350
BstFNI	CGCG	2 cut(s)	68, 1074
BstH2I	RGCGCY	2 cut(s)	146, 594
BstHHI	GCGC	4 cut(s)	145, 593, 1074, 1441
BstKTI	GATC	5 cut(s)	777, 802, 1354, 1506, 1642
BstMAI	GTCTC	4 cut(s)	126, 313, 725, 1220
BstMBI	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
BstMWI	GCNNNNNNNGC	6 cut(s)	65, 571, 764, 878, 977, 1205
BstNI	CCWGG	2 cut(s)	269, 354
BstNSI	RCATGY	1 cut(s)	80
BstPI	GGTNACC	1 cut(s)	271
BstSCI	CCNGG	3 cut(s)	267, 352, 468
BstSFI	CTRYAG	2 cut(s)	902, 1147
BstSLI	GKGCMC	2 cut(s)	256, 1256
BstUI	CGCG	2 cut(s)	68, 1074
BstV1I	GCAGC	6 cut(s)	580, 685, 776, 888, 1184, 1297
BstV2I	GAAGAC	1 cut(s)	950
BstX2I	RGATCY	1 cut(s)	1503
BstXI	CCANNNNNNTGG	2 cut(s)	227, 292
BstYI	RGATCY	1 cut(s)	1503
BsuRI	GGCC	6 cut(s)	188, 284, 878, 908, 980, 1421
BtgI	CCRYGG	1 cut(s)	549
BtsCI	GGATG	7 cut(s)	233, 462, 1032, 1193, 1227, 1339, 1350
BtsIMutI	CAGTG	4 cut(s)	24, 531, 963, 1415
Cac8I	GCNNGC	7 cut(s)	78, 440, 572, 883, 906, 1403, 1488
CaiI	CAGNNNCTG	1 cut(s)	896
CfoI	GCGC	4 cut(s)	145, 593, 1074, 1441
Cfr10I	RCCGGY	1 cut(s)	1421
Cfr13I	GGNCC	4 cut(s)	356, 388, 613, 907
Csp6I	GTAC	1 cut(s)	1582
CspCI	CAANNNNNGTGG	2 cut(s)	381, 416
CviQI	GTAC	1 cut(s)	1582
DpnI	GATC	5 cut(s)	776, 801, 1353, 1505, 1641
DpnII	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
EaeI	YGGCCR	4 cut(s)	186, 282, 876, 1419
Eam1104I	CTCTTC	1 cut(s)	839
EarI	CTCTTC	1 cut(s)	839
Eco130I	CCWWGG	2 cut(s)	988, 1376
Eco24I	GRGCYC	2 cut(s)	92, 1238
Eco47I	GGWCC	3 cut(s)	356, 388, 613
Eco57I	CTGAAG	2 cut(s)	888, 962
Eco72I	CACGTG	1 cut(s)	1622
Eco88I	CYCGRG	1 cut(s)	911
Eco91I	GGTNACC	1 cut(s)	271
EcoO109I	RGGNCCY	1 cut(s)	907
EcoO65I	GGTNACC	1 cut(s)	271
EcoRII	CCWGG	2 cut(s)	267, 352
EcoT14I	CCWWGG	2 cut(s)	988, 1376
EcoT22I	ATGCAT	2 cut(s)	82, 1339
EcoT38I	GRGCYC	2 cut(s)	92, 1238
ErhI	CCWWGG	2 cut(s)	988, 1376
Esp3I	CGTCTC	2 cut(s)	126, 313
FaqI	GGGAC	5 cut(s)	342, 374, 1127, 1133, 1478
FbaI	TGATCA	1 cut(s)	1351
Fnu4HI	GCNGC	8 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286
FokI	GGATG	7 cut(s)	220, 469, 1039, 1200, 1234, 1346, 1357
FriOI	GRGCYC	2 cut(s)	92, 1238
Fsp4HI	GCNGC	8 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286
FspBI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
GlaI	GCGC	4 cut(s)	144, 592, 1073, 1440
GluI	GCNGC	8 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286
GsaI	CCCAGC	1 cut(s)	654
HaeII	RGCGCY	2 cut(s)	146, 594
HaeIII	GGCC	6 cut(s)	188, 284, 878, 908, 980, 1421
HapII	CCGG	2 cut(s)	469, 1422
HhaI	GCGC	4 cut(s)	145, 593, 1074, 1441
Hin6I	GCGC	4 cut(s)	143, 591, 1072, 1439
HinP1I	GCGC	4 cut(s)	143, 591, 1072, 1439
HincII	GTYRAC	1 cut(s)	1534
HindII	GTYRAC	1 cut(s)	1534
HindIII	AAGCTT	3 cut(s)	563, 572, 1388
HinfI	GANTC	1 cut(s)	1591
HpaII	CCGG	2 cut(s)	469, 1422
HphI	GGTGA	5 cut(s)	209, 265, 539, 1285, 1308
Hpy166II	GTNNAC	7 cut(s)	330, 604, 677, 790, 1254, 1534, 1619
Hpy188I	TCNGA	3 cut(s)	94, 955, 1551
Hpy188III	TCNNGA	5 cut(s)	359, 778, 1507, 1588, 1628
Hpy8I	GTNNAC	7 cut(s)	330, 604, 677, 790, 1254, 1534, 1619
HpyAV	CCTTC	5 cut(s)	33, 38, 42, 1133, 1628
HpyCH4III	ACNGT	3 cut(s)	388, 559, 736
HpyCH4IV	ACGT	3 cut(s)	135, 306, 1621
HpyF10VI	GCNNNNNNNGC	6 cut(s)	65, 571, 764, 878, 977, 1205
HpySE526I	ACGT	3 cut(s)	135, 306, 1621
HspAI	GCGC	4 cut(s)	143, 591, 1072, 1439
KpnI	GGTACC	1 cut(s)	1585
Ksp22I	TGATCA	1 cut(s)	1351
Kzo9I	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
LguI	GCTCTTC	1 cut(s)	839
LmnI	GCTCC	3 cut(s)	403, 583, 893
Lsp1109I	GCAGC	6 cut(s)	580, 685, 776, 888, 1184, 1297
LweI	GCATC	6 cut(s)	17, 745, 958, 1324, 1346, 1426
MaeI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
MaeII	ACGT	3 cut(s)	135, 306, 1621
MaeIII	GTNAC	5 cut(s)	271, 527, 625, 641, 961
MalI	GATC	5 cut(s)	776, 801, 1353, 1505, 1641
MboI	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
MboII	GAAGA	6 cut(s)	50, 53, 856, 955, 1288, 1643
MflI	RGATCY	1 cut(s)	1503
MhlI	GDGCHC	4 cut(s)	92, 256, 1238, 1256
MlsI	TGGCCA	2 cut(s)	188, 284
MluCI	AATT	7 cut(s)	213, 300, 710, 1259, 1406, 1567, 1597
MluNI	TGGCCA	2 cut(s)	188, 284
MlyI	GAGTC	1 cut(s)	1585
Mox20I	TGGCCA	2 cut(s)	188, 284
Mph1103I	ATGCAT	2 cut(s)	82, 1339
MroXI	GAANNNNTTC	1 cut(s)	1106
MscI	TGGCCA	2 cut(s)	188, 284
MseI	TTAA	4 cut(s)	561, 684, 1098, 1645
MslI	CAYNNNNRTG	2 cut(s)	278, 1434
Msp20I	TGGCCA	2 cut(s)	188, 284
MspA1I	CMGCKG	3 cut(s)	881, 940, 1175
MspCI	CTTAAG	1 cut(s)	1097
MspI	CCGG	2 cut(s)	469, 1422
MspR9I	CCNGG	3 cut(s)	269, 354, 470
Mva1269I	GAATGC	1 cut(s)	1488
MvaI	CCWGG	2 cut(s)	269, 354
MvnI	CGCG	2 cut(s)	68, 1074
MwoI	GCNNNNNNNGC	6 cut(s)	65, 571, 764, 878, 977, 1205
NciI	CCSGG	1 cut(s)	470
NdeII	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
NlaIV	GGNNCC	7 cut(s)	154, 253, 358, 390, 547, 1505, 1583
NmuCI	GTSAC	5 cut(s)	271, 527, 625, 641, 961
NsiI	ATGCAT	2 cut(s)	82, 1339
NspI	RCATGY	1 cut(s)	80
NspV	TTCGAA	1 cut(s)	824
PaeI	GCATGC	1 cut(s)	80
PaeR7I	CTCGAG	1 cut(s)	911
PciSI	GCTCTTC	1 cut(s)	839
PctI	GAATGC	1 cut(s)	1488
PdmI	GAANNNNTTC	1 cut(s)	1106
PflFI	GACNNNGTC	2 cut(s)	263, 386
PkrI	GCNGC	8 cut(s)	7, 595, 700, 766, 880, 903, 1174, 1287
PleI	GAGTC	1 cut(s)	1585
PmaCI	CACGTG	1 cut(s)	1622
PmlI	CACGTG	1 cut(s)	1622
PpsI	GAGTC	1 cut(s)	1585
Ppu21I	YACGTR	1 cut(s)	1622
PsiI	TTATAA	1 cut(s)	1308
Psp6I	CCWGG	2 cut(s)	267, 352
PspCI	CACGTG	1 cut(s)	1622
PspEI	GGTNACC	1 cut(s)	271
PspFI	CCCAGC	1 cut(s)	650
PspGI	CCWGG	2 cut(s)	267, 352
PspN4I	GGNNCC	7 cut(s)	154, 253, 358, 390, 547, 1505, 1583
PspPI	GGNCC	4 cut(s)	356, 388, 613, 907
PspXI	VCTCGAGB	1 cut(s)	911
PstI	CTGCAG	1 cut(s)	906
PstNI	CAGNNNCTG	1 cut(s)	896
PsuI	RGATCY	1 cut(s)	1503
PsyI	GACNNNGTC	2 cut(s)	263, 386
PvuII	CAGCTG	2 cut(s)	940, 1175
RsaI	GTAC	1 cut(s)	1583
RsaNI	GTAC	1 cut(s)	1582
RseI	CAYNNNNRTG	2 cut(s)	278, 1434
SapI	GCTCTTC	1 cut(s)	839
SaqAI	TTAA	4 cut(s)	561, 684, 1098, 1645
SatI	GCNGC	8 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286
Sau3AI	GATC	5 cut(s)	774, 799, 1351, 1503, 1639
Sau96I	GGNCC	4 cut(s)	356, 388, 613, 907
SchI	GAGTC	1 cut(s)	1585
ScrFI	CCNGG	3 cut(s)	269, 354, 470
SduI	GDGCHC	4 cut(s)	92, 256, 1238, 1256
SfaNI	GCATC	6 cut(s)	17, 745, 958, 1324, 1346, 1426
SfcI	CTRYAG	2 cut(s)	902, 1147
Sfr274I	CTCGAG	1 cut(s)	911
SfuI	TTCGAA	1 cut(s)	824
SinI	GGWCC	3 cut(s)	356, 388, 613
SlaI	CTCGAG	1 cut(s)	911
SmiMI	CAYNNNNRTG	2 cut(s)	278, 1434
SmlI	CTYRAG	4 cut(s)	238, 911, 1097, 1586
SmoI	CTYRAG	4 cut(s)	238, 911, 1097, 1586
SphI	GCATGC	1 cut(s)	80
Sse9I	AATT	7 cut(s)	213, 300, 710, 1259, 1406, 1567, 1597
SsiI	CCGC	4 cut(s)	68, 436, 758, 879
SspMI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
StyD4I	CCNGG	3 cut(s)	267, 352, 468
StyI	CCWWGG	2 cut(s)	988, 1376
TaaI	ACNGT	3 cut(s)	388, 559, 736
TaiI	ACGT	3 cut(s)	138, 309, 1624
TaqI	TCGA	3 cut(s)	824, 912, 1232
TasI	AATT	7 cut(s)	213, 300, 710, 1259, 1406, 1567, 1597
TauI	GCSGC	1 cut(s)	881
Tru1I	TTAA	4 cut(s)	561, 684, 1098, 1645
Tru9I	TTAA	4 cut(s)	561, 684, 1098, 1645
TscAI	CASTG	4 cut(s)	24, 538, 970, 1422
TseFI	GTSAC	5 cut(s)	271, 527, 625, 641, 961
TseI	GCWGC	7 cut(s)	5, 593, 698, 764, 901, 1172, 1285
Tsp45I	GTSAC	5 cut(s)	271, 527, 625, 641, 961
TspDTI	ATGAA	4 cut(s)	195, 998, 1014, 1361
TspRI	CASTG	4 cut(s)	24, 538, 970, 1422
Tth111I	GACNNNGTC	2 cut(s)	263, 386
Vha464I	CTTAAG	1 cut(s)	1097
VneI	GTGCAC	1 cut(s)	1252
VpaK11BI	GGWCC	3 cut(s)	356, 388, 613
XapI	RAATTY	2 cut(s)	213, 1597
XceI	RCATGY	1 cut(s)	80
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1326
XhoI	CTCGAG	1 cut(s)	911
XmaJI	CCTAGG	1 cut(s)	1376
XmnI	GAANNNNTTC	1 cut(s)	1106
XspI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
Zsp2I	ATGCAT	2 cut(s)	82, 1339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.