Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0015497

Physical Location & Seq

Genomic Coordinates Forward (+)

7726830 .. 7729861

Gene Structure 3032 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1106500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGCTGCATCTTACTCAGTGCCTTCAATGATAATGGAAGAAGAAGGAAGGTTTGAGGCAGAAGTCGCAGAGGTGCAGGCATGGTGGGGCTCAGAGAGGTTCAAGCTCACAAAACGACCATACTCAGCTAGAGACGTTGTGGCACTAAGGGGCACCCTAAGACAAAGCTATGGCTCAAATGAAATGGCCAAAAAGCTGTGGAGAACTCTCAAAAATCACCAAGCCAATGGCACATCCTCAAGAACTTTCGGTGCCCTAGACCCCGTCCAGGTCACCATGATGGCCAAGCACTTGGACACAATTTACGTCTCCGGTTGGCAATGCTCGTCCACACACACCAGCACCAATGAGCCTGGTCCCGACCTTGCTGACTACCCTTATGACACCGTCCCAAACAAGGTGGAGCATTTGTTTTTTGCTCAACAATACCACGACCGCAAGCAAAAGGAGGCGAGGATGAGCATGAGCCGGGAGGAGAGGGCTAGAACCCCTTATGTTGATTACTTGAAGCCAATCATTGCTGATGGTGACACCGGTTTTGGTGGCACCACGGCAACTGTTAAGCTTTGCAAGCTTTTCGTGGAGCGAGGCGCTGCTGGTGTTCACATTGAGGACCAGTCATCTGTGACAAAAAAATGTGGTCACATGGCTGGGAAAGTGCTTGTTGCTGTTAGTGAACATATTAACAGACTTGTGGCTGCAAGGCTACAATTTGATGTTATGGGAACTGAGACTGTATTGGTGGCTAGAACCGATGCAGTTGCAGCTACATTGATCCAGACCAATGTGGACACTAGGGATCATCAGTTCATATTGGGGGCTTCTAACCCGAATCTTAGAGGGAAGAGCTTGGCTACCCTTTTGGCTGAAGCAATGGCTGCTGGCAAAACAGGAGCTGAGCTGCAGGCCATTGAGGACAACTGGACTTCAATGGCACAGCTGAAGACATTTTCTGAATGTGTCACTGATGCAATCAAGGCAATGAACCTTGGTGAACACGAAAAAAGGAGGAGATTGAGTGAATGGATGAGCTATTCTAGTCCTGAAAAGTGTTTGTCAAATGAGCAAGCGCGAGAAATTGCTGAGAAGTTGGGGCTTAAGAACCTTTTCTGGGACTGGGACTTGCCTAGAACAAGAGAAGGGTTCTACAGGTTCAAAGGGTCTCTGATGGCAGCGGTCGTGCGTGGCTGGGCTTTTGCTGATCACGCTGACATTATTTGGATGGAGACTTCGAGCCCTGATATGGTGGAGTGCACCCAATTTGCTGAGGGGGTGAAGAATAGGCAGCCTGAAGTCATGTTGGCTTATAATCTTTCACCATCTTTCAACTGGGATGCATCTGGGATGAATGATCAGCAAATGGTGGACTTTATTCCTAGGATCGCCAAGCTTGGCTACTGCTGGCAGTTTATCACGCTGGCAGGATTCCACGCTGATGCGCTTGTGATCGACACTTTTGCGAGGGACTACGCGAGGAGGGGAATGCTGGCTTATGTTGAGAGGATCCAGAGGGAGGAGAGGAACAATGGAGTTGACACACTTGCTCATCAGAAATGGTCTGGTGCTAATTTCTATGATAGGTACCTCAAGACTGTCCAGGGAGGCATTTCTTCCACTGCTGCTATGGGCAAAGGAGTGACTGAAGAGCAATTCAAAGAATCATGGACCAGGCCAGGAACAACTGATCTGAGTGAAGGCAATGTTGTGATTGCCAAGGCAAGACTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004451 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016829 GO:0016830 GO:0016833 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00630 ko01100 ko01110 ko01120 ko01200 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

ICL

Protein Analysis

576

Amino Acids

64.49

Weight (kDa)

6.7

Isoelectric Point (pI)

34.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ICL	PF00463	21 - 551	2.4e-274	Isocitrate lyase family
PEP_mutase	PF13714	170 - 256	2.3e-10	Phosphoenolpyruvate phosphomutase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015497)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21720
fragaria_vesca	FvH4_6g43440
malus_domestica	MD09G1106500.v1.1 MD17G1094300.v1.1
prunus_persica	Prupe.3G219100_v2.0.a1
pyrus_communis	pycom17g09100
rosa_chinensis	RchiOBHm_Chr2g0159821
rosa_laevigata	RLG00000021183
rosa_multiflora	Rmu_sc0016393.1_g000006
rosa_roxburghii	Rroxscaffold_2G00090220
rosa_rugosa	Rorug02G0478900
rosa_samantha	Rh2AG545300 Rh2BG558500 Rh2CG528500 Rh2DG567600
rosa_wichuraiana	Rw2G045100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1308
Acc65I	GGTACC	1 cut(s)	1581
AccB1I	GGYRCC	4 cut(s)	152, 251, 545, 1581
AccII	CGCG	2 cut(s)	1072, 1472
AciI	CCGC	2 cut(s)	436, 1175
AclWI	GGATC	5 cut(s)	769, 807, 1388, 1498, 1511
AcoI	YGGCCR	2 cut(s)	186, 282
AcuI	CTGAAG	4 cut(s)	888, 962, 1311, 1662
AfaI	GTAC	1 cut(s)	1583
AfiI	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
AflII	CTTAAG	1 cut(s)	1097
AgeI	ACCGGT	1 cut(s)	533
AgsI	TTSAA	7 cut(s)	27, 103, 508, 930, 1156, 1327, 1654
AjnI	CCWGG	5 cut(s)	267, 352, 1596, 1667, 1672
AloI	GAACNNNNNNTCC	2 cut(s)	791, 823
Alw21I	GWGCWC	1 cut(s)	1256
Alw26I	GTCTC	5 cut(s)	126, 313, 725, 1167, 1220
Alw44I	GTGCAC	1 cut(s)	1252
AlwI	GGATC	5 cut(s)	769, 807, 1388, 1498, 1511
AlwNI	CAGNNNCTG	1 cut(s)	896
AoxI	GGCC	4 cut(s)	186, 282, 906, 1670
ApaLI	GTGCAC	1 cut(s)	1252
ApeKI	GCWGC	9 cut(s)	5, 593, 698, 764, 878, 901, 1172, 1285, 1619
ArsI	GACNNNNNNTTYG	2 cut(s)	521, 553
AsiGI	ACCGGT	1 cut(s)	533
Asp700I	GAANNNNTTC	1 cut(s)	1106
Asp718I	GGTACC	1 cut(s)	1581
AspA2I	CCTAGG	1 cut(s)	1376
AspLEI	GCGC	3 cut(s)	593, 1072, 1441
AspS9I	GGNCC	3 cut(s)	356, 613, 1665
AsuC2I	CCSGG	1 cut(s)	470
AsuHPI	GGTGA	6 cut(s)	209, 265, 539, 1004, 1285, 1308
AvaII	GGWCC	3 cut(s)	356, 613, 1665
AvrII	CCTAGG	1 cut(s)	1376
BaeGI	GKGCMC	3 cut(s)	155, 256, 1256
BalI	TGGCCA	2 cut(s)	188, 284
BamHI	GGATCC	1 cut(s)	1503
BanI	GGYRCC	4 cut(s)	152, 251, 545, 1581
BanII	GRGCYC	2 cut(s)	92, 1238
BbsI	GAAGAC	1 cut(s)	950
Bbv12I	GWGCWC	1 cut(s)	1256
BbvCI	CCTCAGC	1 cut(s)	1266
BbvI	GCAGC	8 cut(s)	580, 685, 776, 865, 888, 1184, 1297, 1606
BccI	CCATC	5 cut(s)	274, 518, 1162, 1216, 1327
BceAI	ACGGC	1 cut(s)	567
BcgI	CGANNNNNNTGC	6 cut(s)	559, 593, 743, 777, 1439, 1473
BciT130I	CCWGG	5 cut(s)	269, 354, 1598, 1669, 1674
BclI	TGATCA	2 cut(s)	1201, 1351
BcnI	CCSGG	1 cut(s)	470
BcoDI	GTCTC	5 cut(s)	126, 313, 725, 1167, 1220
BfaI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
BfmI	CTRYAG	2 cut(s)	902, 1147
BfoI	RGCGCY	1 cut(s)	594
BfrI	CTTAAG	1 cut(s)	1097
BisI	GCNGC	9 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286, 1620
BlnI	CCTAGG	1 cut(s)	1376
BlpI	GCTNAGC	1 cut(s)	897
BlsI	GCNGC	9 cut(s)	7, 595, 700, 766, 880, 903, 1174, 1287, 1621
Bme1390I	CCNGG	6 cut(s)	269, 354, 470, 1598, 1669, 1674
Bme18I	GGWCC	3 cut(s)	356, 613, 1665
BmgT120I	GGNCC	3 cut(s)	356, 613, 1665
BmiI	GGNNCC	6 cut(s)	154, 253, 358, 547, 1505, 1583
BmrFI	CCNGG	6 cut(s)	269, 354, 470, 1598, 1669, 1674
BmrI	ACTGGG	2 cut(s)	1126, 1339
BmsI	GCATC	6 cut(s)	17, 745, 958, 1324, 1346, 1426
BmuI	ACTGGG	2 cut(s)	1126, 1339
BpiI	GAAGAC	1 cut(s)	950
Bpu10I	CCTNAGC	1 cut(s)	1266
Bpu1102I	GCTNAGC	1 cut(s)	897
BpuEI	CTTGAG	2 cut(s)	223, 1571
BpuMI	CCSGG	1 cut(s)	470
BsaI	GGTCTC	1 cut(s)	1167
BsaJI	CCNNGG	5 cut(s)	549, 988, 1376, 1597, 1713
BsaWI	WCCGGW	2 cut(s)	311, 533
BsaXI	ACNNNNNCTCC	4 cut(s)	395, 425, 1003, 1033
Bsc4I	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
Bse118I	RCCGGY	1 cut(s)	533
Bse1I	ACTGG	4 cut(s)	616, 926, 1121, 1334
Bse3DI	GCAATG	5 cut(s)	326, 516, 879, 987, 1705
BseBI	CCWGG	5 cut(s)	269, 354, 1598, 1669, 1674
BseDI	CCNNGG	5 cut(s)	549, 988, 1376, 1597, 1713
BseGI	GGATG	6 cut(s)	233, 462, 1032, 1227, 1339, 1350
BseLI	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
BseMI	GCAATG	5 cut(s)	326, 516, 879, 987, 1705
BseMII	CTCAG	8 cut(s)	30, 105, 138, 720, 888, 1074, 1257, 1679
BseNI	ACTGG	4 cut(s)	616, 926, 1121, 1334
BseRI	GAGGAG	4 cut(s)	488, 1024, 1489, 1529
BseSI	GKGCMC	3 cut(s)	155, 256, 1256
BseXI	GCAGC	8 cut(s)	580, 685, 776, 865, 888, 1184, 1297, 1606
BseYI	CCCAGC	2 cut(s)	650, 1188
BsgI	GTGCAG	1 cut(s)	95
Bsh1236I	CGCG	2 cut(s)	1072, 1472
Bsh1285I	CGRYCG	2 cut(s)	436, 1179
BshFI	GGCC	4 cut(s)	188, 284, 908, 1672
BshNI	GGYRCC	4 cut(s)	152, 251, 545, 1581
BshTI	ACCGGT	1 cut(s)	533
BsiEI	CGRYCG	2 cut(s)	436, 1179
BsiHKAI	GWGCWC	1 cut(s)	1256
BsiSI	CCGG	3 cut(s)	312, 469, 534
BslFI	GGGAC	5 cut(s)	342, 374, 1127, 1133, 1478
BslI	CCNNNNNNNGG	5 cut(s)	268, 397, 1111, 1243, 1513
BsmAI	GTCTC	5 cut(s)	126, 313, 725, 1167, 1220
BsmBI	CGTCTC	2 cut(s)	126, 313
BsmFI	GGGAC	5 cut(s)	342, 374, 1127, 1133, 1478
BsmI	GAATGC	1 cut(s)	1488
BsnI	GGCC	4 cut(s)	188, 284, 908, 1672
Bso31I	GGTCTC	1 cut(s)	1167
Bsp1286I	GDGCHC	5 cut(s)	92, 155, 256, 1238, 1256
Bsp143I	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
Bsp1720I	GCTNAGC	1 cut(s)	897
BspACI	CCGC	2 cut(s)	436, 1175
BspANI	GGCC	4 cut(s)	188, 284, 908, 1672
BspCNI	CTCAG	8 cut(s)	29, 104, 137, 721, 889, 1075, 1258, 1680
BspFNI	CGCG	2 cut(s)	1072, 1472
BspLI	GGNNCC	6 cut(s)	154, 253, 358, 547, 1505, 1583
BspMAI	CTGCAG	1 cut(s)	906
BspPI	GGATC	5 cut(s)	769, 807, 1388, 1498, 1511
BspQI	GCTCTTC	2 cut(s)	839, 1638
BspT107I	GGYRCC	4 cut(s)	152, 251, 545, 1581
BspTI	CTTAAG	1 cut(s)	1097
BspTNI	GGTCTC	1 cut(s)	1167
BsrDI	GCAATG	5 cut(s)	326, 516, 879, 987, 1705
BsrFI	RCCGGY	1 cut(s)	533
BsrI	ACTGG	4 cut(s)	616, 926, 1121, 1334
BssAI	RCCGGY	1 cut(s)	533
BssECI	CCNNGG	5 cut(s)	549, 988, 1376, 1597, 1713
BssMI	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
BssT1I	CCWWGG	3 cut(s)	988, 1376, 1713
Bst2UI	CCWGG	5 cut(s)	269, 354, 1598, 1669, 1674
Bst4CI	ACNGT	5 cut(s)	388, 559, 736, 1594, 1725
Bst6I	CTCTTC	2 cut(s)	839, 1638
BstAFI	CTTAAG	1 cut(s)	1097
BstAPI	GCANNNNNTGC	1 cut(s)	878
BstC8I	GCNNGC	9 cut(s)	78, 440, 572, 883, 906, 1068, 1403, 1419, 1488
BstDSI	CCRYGG	1 cut(s)	549
BstEII	GGTNACC	1 cut(s)	271
BstF5I	GGATG	6 cut(s)	233, 462, 1032, 1227, 1339, 1350
BstFNI	CGCG	2 cut(s)	1072, 1472
BstH2I	RGCGCY	1 cut(s)	594
BstHHI	GCGC	3 cut(s)	593, 1072, 1441
BstKTI	GATC	8 cut(s)	777, 802, 1204, 1354, 1383, 1449, 1506, 1687
BstMAI	GTCTC	5 cut(s)	126, 313, 725, 1167, 1220
BstMBI	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
BstMCI	CGRYCG	2 cut(s)	436, 1179
BstMWI	GCNNNNNNNGC	6 cut(s)	65, 571, 764, 878, 977, 1205
BstNI	CCWGG	5 cut(s)	269, 354, 1598, 1669, 1674
BstPI	GGTNACC	1 cut(s)	271
BstSCI	CCNGG	6 cut(s)	267, 352, 468, 1596, 1667, 1672
BstSFI	CTRYAG	2 cut(s)	902, 1147
BstSLI	GKGCMC	3 cut(s)	155, 256, 1256
BstUI	CGCG	2 cut(s)	1072, 1472
BstV1I	GCAGC	8 cut(s)	580, 685, 776, 865, 888, 1184, 1297, 1606
BstV2I	GAAGAC	1 cut(s)	950
BstX2I	RGATCY	1 cut(s)	1503
BstXI	CCANNNNNNTGG	2 cut(s)	227, 292
BstYI	RGATCY	1 cut(s)	1503
BsuRI	GGCC	4 cut(s)	188, 284, 908, 1672
BtgI	CCRYGG	1 cut(s)	549
BtsCI	GGATG	6 cut(s)	233, 462, 1032, 1227, 1339, 1350
BtsI	GCAGTG	1 cut(s)	1614
BtsIMutI	CAGTG	3 cut(s)	24, 963, 1614
Cac8I	GCNNGC	9 cut(s)	78, 440, 572, 883, 906, 1068, 1403, 1419, 1488
CaiI	CAGNNNCTG	1 cut(s)	896
CfoI	GCGC	3 cut(s)	593, 1072, 1441
Cfr10I	RCCGGY	1 cut(s)	533
Cfr13I	GGNCC	3 cut(s)	356, 613, 1665
Csp6I	GTAC	1 cut(s)	1582
CspAI	ACCGGT	1 cut(s)	533
CspCI	CAANNNNNGTGG	2 cut(s)	381, 416
CviAII	CATG	6 cut(s)	81, 277, 463, 646, 1297, 1662
CviQI	GTAC	1 cut(s)	1582
DpnI	GATC	8 cut(s)	776, 801, 1203, 1353, 1382, 1448, 1505, 1686
DpnII	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
EaeI	YGGCCR	2 cut(s)	186, 282
Eam1104I	CTCTTC	2 cut(s)	839, 1638
EarI	CTCTTC	2 cut(s)	839, 1638
Eco130I	CCWWGG	3 cut(s)	988, 1376, 1713
Eco24I	GRGCYC	2 cut(s)	92, 1238
Eco31I	GGTCTC	1 cut(s)	1167
Eco47I	GGWCC	3 cut(s)	356, 613, 1665
Eco57I	CTGAAG	4 cut(s)	888, 962, 1311, 1662
Eco91I	GGTNACC	1 cut(s)	271
EcoO65I	GGTNACC	1 cut(s)	271
EcoRII	CCWGG	5 cut(s)	267, 352, 1596, 1667, 1672
EcoT14I	CCWWGG	3 cut(s)	988, 1376, 1713
EcoT22I	ATGCAT	1 cut(s)	1339
EcoT38I	GRGCYC	2 cut(s)	92, 1238
ErhI	CCWWGG	3 cut(s)	988, 1376, 1713
Esp3I	CGTCTC	2 cut(s)	126, 313
FaeI	CATG	6 cut(s)	84, 280, 466, 649, 1300, 1665
FaqI	GGGAC	5 cut(s)	342, 374, 1127, 1133, 1478
FatI	CATG	6 cut(s)	80, 276, 462, 645, 1296, 1661
FbaI	TGATCA	2 cut(s)	1201, 1351
Fnu4HI	GCNGC	9 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286, 1620
FokI	GGATG	6 cut(s)	220, 469, 1039, 1234, 1346, 1357
FriOI	GRGCYC	2 cut(s)	92, 1238
Fsp4HI	GCNGC	9 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286, 1620
FspBI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
GlaI	GCGC	3 cut(s)	592, 1071, 1440
GluI	GCNGC	9 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286, 1620
GsaI	CCCAGC	2 cut(s)	654, 1192
HaeII	RGCGCY	1 cut(s)	594
HaeIII	GGCC	4 cut(s)	188, 284, 908, 1672
HapII	CCGG	3 cut(s)	312, 469, 534
HhaI	GCGC	3 cut(s)	593, 1072, 1441
Hin1II	CATG	6 cut(s)	84, 280, 466, 649, 1300, 1665
Hin6I	GCGC	3 cut(s)	591, 1070, 1439
HinP1I	GCGC	3 cut(s)	591, 1070, 1439
HincII	GTYRAC	1 cut(s)	1534
HindII	GTYRAC	1 cut(s)	1534
HindIII	AAGCTT	3 cut(s)	563, 572, 1388
HinfI	GANTC	3 cut(s)	832, 1425, 1658
HpaII	CCGG	3 cut(s)	312, 469, 534
HphI	GGTGA	6 cut(s)	209, 265, 539, 1004, 1285, 1308
Hpy166II	GTNNAC	8 cut(s)	330, 604, 677, 790, 995, 1254, 1366, 1534
Hpy188I	TCNGA	5 cut(s)	94, 955, 1167, 1551, 1689
Hpy188III	TCNNGA	6 cut(s)	240, 359, 778, 1043, 1507, 1588
Hpy8I	GTNNAC	8 cut(s)	330, 604, 677, 790, 995, 1254, 1366, 1534
HpyAV	CCTTC	5 cut(s)	33, 38, 42, 1133, 1688
HpyCH4III	ACNGT	5 cut(s)	388, 559, 736, 1594, 1725
HpyCH4IV	ACGT	2 cut(s)	135, 306
HpyF10VI	GCNNNNNNNGC	6 cut(s)	65, 571, 764, 878, 977, 1205
HpySE526I	ACGT	2 cut(s)	135, 306
Hsp92II	CATG	6 cut(s)	84, 280, 466, 649, 1300, 1665
HspAI	GCGC	3 cut(s)	591, 1070, 1439
KpnI	GGTACC	1 cut(s)	1585
Ksp22I	TGATCA	2 cut(s)	1201, 1351
Kzo9I	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
LguI	GCTCTTC	2 cut(s)	839, 1638
LmnI	GCTCC	3 cut(s)	403, 583, 893
Lsp1109I	GCAGC	8 cut(s)	580, 685, 776, 865, 888, 1184, 1297, 1606
LweI	GCATC	6 cut(s)	17, 745, 958, 1324, 1346, 1426
MaeI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
MaeII	ACGT	2 cut(s)	135, 306
MaeIII	GTNAC	6 cut(s)	271, 527, 625, 641, 961, 1636
MalI	GATC	8 cut(s)	776, 801, 1203, 1353, 1382, 1448, 1505, 1686
MboI	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
MboII	GAAGA	7 cut(s)	50, 53, 856, 955, 1288, 1602, 1655
MflI	RGATCY	1 cut(s)	1503
MhlI	GDGCHC	5 cut(s)	92, 155, 256, 1238, 1256
MlsI	TGGCCA	2 cut(s)	188, 284
MluCI	AATT	6 cut(s)	300, 710, 1077, 1259, 1567, 1649
MluNI	TGGCCA	2 cut(s)	188, 284
Mox20I	TGGCCA	2 cut(s)	188, 284
Mph1103I	ATGCAT	1 cut(s)	1339
MroXI	GAANNNNTTC	1 cut(s)	1106
MscI	TGGCCA	2 cut(s)	188, 284
MseI	TTAA	3 cut(s)	561, 684, 1098
MslI	CAYNNNNRTG	2 cut(s)	278, 1434
Msp20I	TGGCCA	2 cut(s)	188, 284
MspA1I	CMGCKG	2 cut(s)	940, 1175
MspCI	CTTAAG	1 cut(s)	1097
MspI	CCGG	3 cut(s)	312, 469, 534
MspR9I	CCNGG	6 cut(s)	269, 354, 470, 1598, 1669, 1674
Mva1269I	GAATGC	1 cut(s)	1488
MvaI	CCWGG	5 cut(s)	269, 354, 1598, 1669, 1674
MvnI	CGCG	2 cut(s)	1072, 1472
MwoI	GCNNNNNNNGC	6 cut(s)	65, 571, 764, 878, 977, 1205
NciI	CCSGG	1 cut(s)	470
NdeII	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
NlaIII	CATG	6 cut(s)	84, 280, 466, 649, 1300, 1665
NlaIV	GGNNCC	6 cut(s)	154, 253, 358, 547, 1505, 1583
NmuCI	GTSAC	6 cut(s)	271, 527, 625, 641, 961, 1636
NsiI	ATGCAT	1 cut(s)	1339
PciSI	GCTCTTC	2 cut(s)	839, 1638
PctI	GAATGC	1 cut(s)	1488
PdmI	GAANNNNTTC	1 cut(s)	1106
PfeI	GAWTC	3 cut(s)	832, 1425, 1658
PflFI	GACNNNGTC	2 cut(s)	263, 386
PinAI	ACCGGT	1 cut(s)	533
PkrI	GCNGC	9 cut(s)	7, 595, 700, 766, 880, 903, 1174, 1287, 1621
PsiI	TTATAA	1 cut(s)	1308
Psp6I	CCWGG	5 cut(s)	267, 352, 1596, 1667, 1672
PspEI	GGTNACC	1 cut(s)	271
PspFI	CCCAGC	2 cut(s)	650, 1188
PspGI	CCWGG	5 cut(s)	267, 352, 1596, 1667, 1672
PspN4I	GGNNCC	6 cut(s)	154, 253, 358, 547, 1505, 1583
PspPI	GGNCC	3 cut(s)	356, 613, 1665
PstI	CTGCAG	1 cut(s)	906
PstNI	CAGNNNCTG	1 cut(s)	896
PsuI	RGATCY	1 cut(s)	1503
PsyI	GACNNNGTC	2 cut(s)	263, 386
PvuII	CAGCTG	1 cut(s)	940
RsaI	GTAC	1 cut(s)	1583
RsaNI	GTAC	1 cut(s)	1582
RseI	CAYNNNNRTG	2 cut(s)	278, 1434
SapI	GCTCTTC	2 cut(s)	839, 1638
SaqAI	TTAA	3 cut(s)	561, 684, 1098
SatI	GCNGC	9 cut(s)	6, 594, 699, 765, 879, 902, 1173, 1286, 1620
Sau3AI	GATC	8 cut(s)	774, 799, 1201, 1351, 1380, 1446, 1503, 1684
Sau96I	GGNCC	3 cut(s)	356, 613, 1665
ScrFI	CCNGG	6 cut(s)	269, 354, 470, 1598, 1669, 1674
SduI	GDGCHC	5 cut(s)	92, 155, 256, 1238, 1256
SfaNI	GCATC	6 cut(s)	17, 745, 958, 1324, 1346, 1426
SfcI	CTRYAG	2 cut(s)	902, 1147
SinI	GGWCC	3 cut(s)	356, 613, 1665
SmiMI	CAYNNNNRTG	2 cut(s)	278, 1434
SmlI	CTYRAG	3 cut(s)	238, 1097, 1586
SmoI	CTYRAG	3 cut(s)	238, 1097, 1586
Sse9I	AATT	6 cut(s)	300, 710, 1077, 1259, 1567, 1649
SsiI	CCGC	2 cut(s)	436, 1175
SspMI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
StyD4I	CCNGG	6 cut(s)	267, 352, 468, 1596, 1667, 1672
StyI	CCWWGG	3 cut(s)	988, 1376, 1713
TaaI	ACNGT	5 cut(s)	388, 559, 736, 1594, 1725
TaiI	ACGT	2 cut(s)	138, 309
TaqI	TCGA	2 cut(s)	1232, 1449
TasI	AATT	6 cut(s)	300, 710, 1077, 1259, 1567, 1649
TfiI	GAWTC	3 cut(s)	832, 1425, 1658
Tru1I	TTAA	3 cut(s)	561, 684, 1098
Tru9I	TTAA	3 cut(s)	561, 684, 1098
TscAI	CASTG	3 cut(s)	24, 970, 1621
TseFI	GTSAC	6 cut(s)	271, 527, 625, 641, 961, 1636
TseI	GCWGC	9 cut(s)	5, 593, 698, 764, 878, 901, 1172, 1285, 1619
Tsp45I	GTSAC	6 cut(s)	271, 527, 625, 641, 961, 1636
TspDTI	ATGAA	4 cut(s)	195, 799, 998, 1361
TspRI	CASTG	3 cut(s)	24, 970, 1621
Tth111I	GACNNNGTC	2 cut(s)	263, 386
Vha464I	CTTAAG	1 cut(s)	1097
VneI	GTGCAC	1 cut(s)	1252
VpaK11BI	GGWCC	3 cut(s)	356, 613, 1665
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1326, 1621
XmaJI	CCTAGG	1 cut(s)	1376
XmnI	GAANNNNTTC	1 cut(s)	1106
XspI	CTAG	8 cut(s)	129, 257, 483, 747, 795, 1038, 1128, 1377
Zsp2I	ATGCAT	1 cut(s)	1339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.