FvH4_6g53120

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004680
Physical Location & Seq
Forward (+)
39001544 .. 39007247
5704 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53120.t2

Sequence Viewer

Length: 3156 bp
ATGGTGATAGGATTAAAGGCGAAGAATCACAGAGGTCCTACTGTTCAGATTGATTACCTAGTCCATATACTTGAGATTAAGCCTTGGCCACCGTCGCAGTCGCTTAAATCGCTGCGGTCCGTGTTGATTCAATGGGAGAATGGAGAACGCAGCTCCGGGATGACTAATGCGGTTGTGCCGTCGATTGGTTCGGTTGTTGGAGAGGGGAGAATTGAGTTCAATGAGTCTTTTAAGCTGCCGGTGACTTTGCTGAGGGATGTAGCTGTGAAAGGCGGTGTGAAAGGAAGCGGTGAAGGTGATGCATTTTTGAAGAATTGCTTGGAGTTGAATTTGTATGAGCCTCGGAGGGATAAGACTGCGAAAGGGCAGCTGTTGGCGACTGCGGTTGTAGATTTAGCTGATTATGGTGTTGTGAGAGAGAGTGTGTGTGTAAGTGCTCCGATGAATAGTAAGAGGAGCTTTAAGAACACGGATAAACCGATTTTGTATATGAAGATTCAGCCGTTTAAGAAGGGTCGCCCCAGCTCCTCGTCTAGGGACAGCTTGTCAAGAGCGGTTTCACTGGACAAGGCGGGTGGTGAATCTGTTTCGGCATTGATGGATGAAGAGTACGCTGATGAAGCGGAAGTTGCCTCTTTTACAGATGACGATGTATCCTCCCACTCATCTCAGACAGTATCTTCTTCTATAGAAACAAGTCGTGGGGTGTCTTCTCCGAAAGAAGAGACTGCACAAGCCAATATGCCTCATAGCAATGAAGGAGATAATGCAAAGCATTCCTTAGCCTCAAACCTAGGTTCTGAAAAATCAAGATCAATACCTCAGAGTGCACCACAAGAATATCTGAAGGGAAGTTCATCATGCTCCTCATCGGTTGACTTGTGTTCTGACCCTGGAAGCCCAGTAAATGGTCATGCTTCTGTAGCTTACTCTCGCAGCTCTAGTTTCACAACAGTCTCAAAAACTGCTGGTTCCCTGATTGTCTCTTCTTCTTCATCATCCTCGTTGAATGAAAATGCGGAGGAGTCCAATATCAGCATGCGAAGCAATGGCCATGCACATGCGGAGGAGGTAAATGATAAGGTTGTTAATGGAACAATTAAAGTCACTGCTGATATTCAAGAGAGTAGGAAGGATGATGAGAAGGCTCAGCAAATTAGCGGAGATTCCGTGGAGGCTGCTGCTGATGATGATAAATACGATAATGAGGATAAAGACAGACAGAAACAAGAAGAAAATGGTGATGAAAGACAAAATTGTGATGAGGAGAACCATTCAGGGGAAGGTGAACCATACATCGCAGGTCATGCTAATGGAAAAGATGTTCTGCTAGGAATGAATGAAATTATTGTGAGCAATGACAAACTGAAGCCTGTCAAGTCTGTTAGGTCAATTGCTGACTTAAGTAAGAATATATCATCTAGGAATGATCAGCATGTAGAAGTAAAGGATGGTGTTCAGGGAGATGCACAGAAGAGTGCAGGTGTTTCAGGAAACCTCAGAGTTAAGGAAAGGAAAGAGGCTAAGGTGTATCCCAAGGATACAAGGAGTGTCATTTTAGAAAGCAAGGTTAATCAGTTGGAACACAAAATAAAGATGCTTGAGGGAGAGCTAAGAGAAGCTGCAGCTGTTGAGTCTGCTCTTTATTCAGTAGTTGCTGAGCATGGAAGTTCCATGAGTAAGGTCCATGCTCCAGCTCGGCGCCTTTCTAGGTTGTATCTTCATGCTTGTGGAGAAACTTCCCGTTCTAGGAGAGCCAGTGCAGCTAGAAGTGTTGTTTCAGGACTAGTTTTGGTTTCAAAAGCTTGTGGGAATGATGTTCCAAGGTTAACATTTTGGCTCTCAAACTCGATTGTCTTGAGAACAATTATAAGCCAGGCTATTGGGGACCCTGCACTTCCCAAGTCTGCCAGATCGTCTATTGACAGAAATGGTGCTGAAAAAGTCAAACATATGGCTTCATCTCCATTAAAATGGGAGGCTCCATCCTCTGGGAAAAAACAAGGGATGAAACTATTGAATGGAAGTTTTGGCGACTGGGAGAACCCAAATACGTTTATGTCCACACTGGAGAAAATTGAATCATGGATCTTCTCCCGAATAGTTGAATCTATCTGGTGGCAGACTTTGACACCACATATGCAGTCTGTCACTGCAAAGGCAACTGATGAAGGGTCACGAAAGAATTATAGAAGGACATCTGGTTCAGTTGATCAAGAGCAAAGTGACTTTTCATTAGACCTTTGGAAGAAGGCTTTCAGGGATGCTTGTGAACGACTTTGTCCAGTAAGAGCTGGTGGGCATGAATGTGGCTGCTTGCCTTTGTTATCTAGATTGGTCATGGAGCAATCTGTGGCTAGATTAGATGTGGCAATGTTCAATGCCATTCTCCGGGAATCTTCTGACGAGATCCCCAGCGATCCCGTCTCTGACCCCATCAGTGATTTGAAGGTTCTTCCAATTCCAGCTGGGAAATCTAGTTTTGGGGCAGGTGCACAGCTGAAGAGTGTGATAGGGAACTGGTCCAGATGGTTAACTGACCTTTTTGGTATCGATGATGATGACTCATTTGAAGATGTAAATGGAGATGATGATAATGATGAAAGACATGATACATCCTTCAAGTCCTTTCATCTGCTTAATGCATTGAGTGATCTCATGATGCTTCCAAAGGACATGCTCTTAAGCAAATCCATCAGAAAAGAGGTATGCCCTACATTTGCTGCACCACTGATCAAAAGGATTCTTGACAACTTTGTCCCCGACGAATTTTGCACGGACCCAATTCCAGACATTGTGCTTAAAAACCTGGAGTCTGAGGACACTCATGAGATTGGGGAGGAGGCTCTCAGAAACATACCATGCACTGGACTTGGTACAGTCTATTTGCCCCCCTCAACAGCTTTGGTTGCAAATATAATTGGAGATGGTGGAGGCCAATCACAGCTGAGAAGAAGCGGATCTTCAGTGGTCAGGAAATCATACACCAGCGATGATGAGCTCGATGAACTGAATTCACCCTTGGCCTCGATCTTCATAGGTTCTTCTGGGTCTTCACCAGTTGCATCGAAGCTCAACTGGGTCCCAAAGGGAAACATTAATCAAAATGCTGTCAGATATGAACTCCTTCGGGATGTTTGGATGAATAGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1052

Amino Acids

114.31

Weight (kDa)

5.52

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 16 - 167 5.7e-08 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1871
AarI CACCTGC 2 cut(s) 1472, 2480
AasI GACNNNNNNGTC 1 cut(s) 2756
Acc36I ACCTGC 3 cut(s) 1292, 1472, 2480
AccB1I GGYRCC 1 cut(s) 1701
AccB7I CCANNNNNTGG 3 cut(s) 908, 1991, 2867
AccBSI CCGCTC 1 cut(s) 554
AclWI GGATC 4 cut(s) 2096, 2404, 2414, 2968
AcoI YGGCCR 2 cut(s) 86, 1051
AcsI RAATTY 3 cut(s) 328, 2768, 3013
AcuI CTGAAG 4 cut(s) 866, 1388, 2522, 2949
AcyI GRCGYC 1 cut(s) 1702
AfaI GTAC 2 cut(s) 611, 2878
AfiI CCNNNNNNNGG 8 cut(s) 185, 534, 908, 1279, 1749, 1991, 2391, 2867
AflII CTTAAG 2 cut(s) 1402, 2683
AhdI GACNNNNNGTC 1 cut(s) 544
AhlI ACTAGT 1 cut(s) 1786
AjnI CCWGG 3 cut(s) 892, 1875, 2808
AjuI GAANNNNNNNTTGG 4 cut(s) 302, 334, 3005, 3037
AloI GAACNNNNNNTCC 4 cut(s) 1308, 1340, 2188, 2220
Alw21I GWGCWC 4 cut(s) 439, 832, 2497, 3003
Alw26I GTCTC 4 cut(s) 719, 961, 988, 2431
Alw44I GTGCAC 2 cut(s) 828, 2493
AlwI GGATC 4 cut(s) 2096, 2404, 2414, 2968
AoxI GGCC 4 cut(s) 86, 1051, 2935, 3024
ApaLI GTGCAC 2 cut(s) 828, 2493
ApoI RAATTY 3 cut(s) 328, 2768, 3013
AseI ATTAAT 1 cut(s) 3099
Asp700I GAANNNNTTC 2 cut(s) 2255, 3126
AspA2I CCTAGG 1 cut(s) 793
AspLEI GCGC 1 cut(s) 1704
AspS9I GGNCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
AsuC2I CCSGG 2 cut(s) 157, 2393
AsuHPI GGTGA 9 cut(s) 16, 253, 302, 308, 590, 1253, 1298, 3009, 3048
AvaII GGWCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
AvrII CCTAGG 1 cut(s) 793
BaeGI GKGCMC 2 cut(s) 832, 2497
BalI TGGCCA 2 cut(s) 88, 1053
BanI GGYRCC 1 cut(s) 1701
BanII GRGCYC 1 cut(s) 3003
BbsI GAAGAC 2 cut(s) 702, 3045
Bbv12I GWGCWC 4 cut(s) 439, 832, 2497, 3003
BbvCI CCTCAGC 1 cut(s) 251
BccI CCATC 7 cut(s) 592, 1445, 1993, 2444, 2523, 2702, 2921
BceAI ACGGC 2 cut(s) 163, 487
BciT130I CCWGG 3 cut(s) 894, 1877, 2810
BciVI GTATCC 3 cut(s) 664, 1534, 1542
BclI TGATCA 3 cut(s) 1429, 2212, 2733
BcnI CCSGG 2 cut(s) 157, 2393
BcoDI GTCTC 4 cut(s) 719, 961, 988, 2431
BcuI ACTAGT 1 cut(s) 1786
BfmI CTRYAG 3 cut(s) 687, 921, 1623
BfoI RGCGCY 1 cut(s) 1705
BfrI CTTAAG 2 cut(s) 1402, 2683
BfuAI ACCTGC 3 cut(s) 1292, 1472, 2480
BfuI GTATCC 3 cut(s) 664, 1534, 1542
BlnI CCTAGG 1 cut(s) 793
BlpI GCTNAGC 2 cut(s) 1149, 1659
Bme1390I CCNGG 5 cut(s) 157, 894, 1877, 2393, 2810
Bme18I GGWCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
BmeRI GACNNNNNGTC 1 cut(s) 544
BmgT120I GGNCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
BmiI GGNNCC 8 cut(s) 973, 1703, 1889, 1890, 1983, 2781, 3083, 3084
BmrFI CCNGG 5 cut(s) 157, 894, 1877, 2393, 2810
BmrI ACTGGG 3 cut(s) 896, 2047, 3088
BmsI GCATC 6 cut(s) 289, 1456, 1587, 2254, 2652, 3074
BmuI ACTGGG 3 cut(s) 896, 2047, 3088
BpiI GAAGAC 2 cut(s) 702, 3045
BpmI CTGGAG 3 cut(s) 1677, 2090, 2831
Bpu10I CCTNAGC 3 cut(s) 251, 781, 1524
Bpu1102I GCTNAGC 2 cut(s) 1149, 1659
BpuEI CTTGAG 3 cut(s) 92, 1622, 1879
BpuMI CCSGG 2 cut(s) 157, 2393
Bsa29I ATCGAT 1 cut(s) 2553
BsaHI GRCGYC 1 cut(s) 1702
BsaJI CCNNGG 8 cut(s) 83, 341, 793, 892, 1170, 1536, 1823, 3021
BsaXI ACNNNNNCTCC 4 cut(s) 1155, 1185, 1744, 1774
Bsc4I CCNNNNNNNGG 8 cut(s) 185, 534, 908, 1279, 1749, 1991, 2391, 2867
Bse118I RCCGGY 1 cut(s) 238
Bse3DI GCAATG 4 cut(s) 760, 1054, 1363, 2379
BseBI CCWGG 3 cut(s) 894, 1877, 2810
BseCI ATCGAT 1 cut(s) 2553
BseDI CCNNGG 8 cut(s) 83, 341, 793, 892, 1170, 1536, 1823, 3021
BseLI CCNNNNNNNGG 8 cut(s) 185, 534, 908, 1279, 1749, 1991, 2391, 2867
BseMI GCAATG 4 cut(s) 760, 1054, 1363, 2379
BseMII CTCAG 9 cut(s) 242, 683, 836, 1163, 1513, 1650, 2808, 2863, 2939
BseRI GAGGAG 7 cut(s) 469, 517, 856, 1037, 1082, 1280, 2855
BseSI GKGCMC 2 cut(s) 832, 2497
BseYI CCCAGC 3 cut(s) 521, 2414, 2468
BsgI GTGCAG 5 cut(s) 714, 1500, 1782, 1878, 2709
BshFI GGCC 4 cut(s) 88, 1053, 2937, 3026
BshNI GGYRCC 1 cut(s) 1701
BshVI ATCGAT 1 cut(s) 2553
BsiHKAI GWGCWC 4 cut(s) 439, 832, 2497, 3003
BsiSI CCGG 3 cut(s) 156, 239, 2392
BslFI GGGAC 4 cut(s) 551, 1901, 2744, 3068
BslI CCNNNNNNNGG 8 cut(s) 185, 534, 908, 1279, 1749, 1991, 2391, 2867
BsmAI GTCTC 4 cut(s) 719, 961, 988, 2431
BsmBI CGTCTC 1 cut(s) 2431
BsmFI GGGAC 4 cut(s) 551, 1901, 2744, 3068
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 4 cut(s) 88, 1053, 2937, 3026
Bsp1286I GDGCHC 4 cut(s) 439, 832, 2497, 3003
Bsp1720I GCTNAGC 2 cut(s) 1149, 1659
BspANI GGCC 4 cut(s) 88, 1053, 2937, 3026
BspCNI CTCAG 9 cut(s) 243, 682, 835, 1162, 1512, 1651, 2809, 2862, 2940
BspDI ATCGAT 1 cut(s) 2553
BspHI TCATGA 2 cut(s) 2658, 2827
BspLI GGNNCC 8 cut(s) 973, 1703, 1889, 1890, 1983, 2781, 3083, 3084
BspMAI CTGCAG 1 cut(s) 1627
BspMI ACCTGC 3 cut(s) 1292, 1472, 2480
BspPI GGATC 4 cut(s) 2096, 2404, 2414, 2968
BspT107I GGYRCC 1 cut(s) 1701
BspTI CTTAAG 2 cut(s) 1402, 2683
BsrBI CCGCTC 1 cut(s) 554
BsrDI GCAATG 4 cut(s) 760, 1054, 1363, 2379
BsrFI RCCGGY 1 cut(s) 238
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 8 cut(s) 83, 341, 793, 892, 1170, 1536, 1823, 3021
BssNI GRCGYC 1 cut(s) 1702
BssT1I CCWWGG 5 cut(s) 83, 793, 1536, 1823, 3021
Bst2UI CCWGG 3 cut(s) 894, 1877, 2810
Bst4CI ACNGT 5 cut(s) 43, 93, 676, 955, 2881
Bst6I CTCTTC 5 cut(s) 600, 717, 991, 1469, 2498
BstACI GRCGYC 1 cut(s) 1702
BstAFI CTTAAG 2 cut(s) 1402, 2683
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstC8I GCNNGC 2 cut(s) 1040, 2318
BstDSI CCRYGG 1 cut(s) 1170
BstENI CCTNNNNNAGG 1 cut(s) 532
BstH2I RGCGCY 1 cut(s) 1705
BstHHI GCGC 1 cut(s) 1704
BstMAI GTCTC 4 cut(s) 719, 961, 988, 2431
BstMWI GCNNNNNNNGC 9 cut(s) 94, 109, 620, 629, 923, 1044, 1307, 1763, 2909
BstNI CCWGG 3 cut(s) 894, 1877, 2810
BstNSI RCATGY 4 cut(s) 1042, 1064, 1439, 2680
BstSCI CCNGG 5 cut(s) 155, 892, 1875, 2391, 2808
BstSFI CTRYAG 3 cut(s) 687, 921, 1623
BstSLI GKGCMC 2 cut(s) 832, 2497
BstV2I GAAGAC 2 cut(s) 702, 3045
BstX2I RGATCY 3 cut(s) 2088, 2409, 2960
BstXI CCANNNNNNTGG 2 cut(s) 1883, 1974
BstYI RGATCY 3 cut(s) 2088, 2409, 2960
Bsu15I ATCGAT 1 cut(s) 2553
BsuI GTATCC 3 cut(s) 664, 1534, 1542
BsuRI GGCC 4 cut(s) 88, 1053, 2937, 3026
BsuTUI ATCGAT 1 cut(s) 2553
BtgI CCRYGG 1 cut(s) 1170
BtgZI GCGATG 2 cut(s) 1282, 3006
BtsI GCAGTG 2 cut(s) 1107, 2151
BtsIMutI CAGTG 9 cut(s) 560, 1107, 1765, 2066, 2151, 2446, 2729, 2865, 2973
BveI ACCTGC 3 cut(s) 1292, 1472, 2480
Cac8I GCNNGC 2 cut(s) 1040, 2318
CciI TCATGA 2 cut(s) 2658, 2827
CfoI GCGC 1 cut(s) 1704
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
ClaI ATCGAT 1 cut(s) 2553
CpoI CGGWCCG 1 cut(s) 117
Csp6I GTAC 2 cut(s) 610, 2877
CspCI CAANNNNNGTGG 2 cut(s) 556, 591
CspI CGGWCCG 1 cut(s) 117
CviQI GTAC 2 cut(s) 610, 2877
DinI GGCGCC 1 cut(s) 1703
DrdI GACNNNNNNGTC 1 cut(s) 2756
DriI GACNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 1 cut(s) 2756
EaeI YGGCCR 2 cut(s) 86, 1051
Eam1104I CTCTTC 5 cut(s) 600, 717, 991, 1469, 2498
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 5 cut(s) 600, 717, 991, 1469, 2498
Ecl136II GAGCTC 1 cut(s) 3001
Eco130I CCWWGG 5 cut(s) 83, 793, 1536, 1823, 3021
Eco24I GRGCYC 1 cut(s) 3003
Eco47I GGWCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
Eco53kI GAGCTC 1 cut(s) 3001
Eco57I CTGAAG 4 cut(s) 866, 1388, 2522, 2949
EcoICRI GAGCTC 1 cut(s) 3001
EcoNI CCTNNNNNAGG 1 cut(s) 532
EcoO109I RGGNCCY 3 cut(s) 35, 1888, 3082
EcoRI GAATTC 1 cut(s) 3013
EcoRII CCWGG 3 cut(s) 892, 1875, 2808
EcoT14I CCWWGG 5 cut(s) 83, 793, 1536, 1823, 3021
EcoT22I ATGCAT 2 cut(s) 304, 2647
EcoT38I GRGCYC 1 cut(s) 3003
EgeI GGCGCC 1 cut(s) 1703
EheI GGCGCC 1 cut(s) 1703
ErhI CCWWGG 5 cut(s) 83, 793, 1536, 1823, 3021
Esp3I CGTCTC 1 cut(s) 2431
FalI AAGNNNNNCTT 8 cut(s) 302, 334, 443, 475, 764, 796, 2947, 2979
FaqI GGGAC 4 cut(s) 551, 1901, 2744, 3068
FauI CCCGC 1 cut(s) 565
FauNDI CATATG 2 cut(s) 1953, 2139
FbaI TGATCA 3 cut(s) 1429, 2212, 2733
FriOI GRGCYC 1 cut(s) 3003
GlaI GCGC 1 cut(s) 1703
GsaI CCCAGC 3 cut(s) 525, 2418, 2472
GsuI CTGGAG 3 cut(s) 1677, 2090, 2831
HaeII RGCGCY 1 cut(s) 1705
HaeIII GGCC 4 cut(s) 88, 1053, 2937, 3026
HapII CCGG 3 cut(s) 156, 239, 2392
HhaI GCGC 1 cut(s) 1704
Hin1I GRCGYC 1 cut(s) 1702
Hin6I GCGC 1 cut(s) 1702
HinP1I GCGC 1 cut(s) 1702
HincII GTYRAC 3 cut(s) 877, 1830, 2535
HindII GTYRAC 3 cut(s) 877, 1830, 2535
HindIII AAGCTT 1 cut(s) 1803
HpaI GTTAAC 2 cut(s) 1830, 2535
HpaII CCGG 3 cut(s) 156, 239, 2392
HphI GGTGA 9 cut(s) 16, 253, 302, 308, 590, 1253, 1298, 3009, 3048
Hpy166II GTNNAC 8 cut(s) 830, 877, 1289, 1830, 2064, 2273, 2495, 2535
Hpy8I GTNNAC 8 cut(s) 830, 877, 1289, 1830, 2064, 2273, 2495, 2535
Hpy99I CGWCG 3 cut(s) 97, 184, 2768
HpyCH4III ACNGT 5 cut(s) 43, 93, 676, 955, 2881
HpyCH4IV ACGT 1 cut(s) 2054
HpyF10VI GCNNNNNNNGC 9 cut(s) 94, 109, 620, 629, 923, 1044, 1307, 1763, 2909
HpySE526I ACGT 1 cut(s) 2054
Hsp92I GRCGYC 1 cut(s) 1702
HspAI GCGC 1 cut(s) 1702
KasI GGCGCC 1 cut(s) 1701
KflI GGGWCCC 2 cut(s) 1888, 3082
Ksp22I TGATCA 3 cut(s) 1429, 2212, 2733
KspAI GTTAAC 2 cut(s) 1830, 2535
LmnI GCTCC 8 cut(s) 158, 442, 456, 530, 869, 1696, 1987, 2344
LweI GCATC 6 cut(s) 289, 1456, 1587, 2254, 2652, 3074
MaeII ACGT 1 cut(s) 2054
MaeIII GTNAC 5 cut(s) 241, 1105, 2149, 2175, 2225
MbiI CCGCTC 1 cut(s) 554
MfeI CAATTG 1 cut(s) 1392
MflI RGATCY 3 cut(s) 2088, 2409, 2960
MhlI GDGCHC 4 cut(s) 439, 832, 2497, 3003
MlsI TGGCCA 2 cut(s) 88, 1053
MluNI TGGCCA 2 cut(s) 88, 1053
Mly113I GGCGCC 1 cut(s) 1702
MlyI GAGTC 5 cut(s) 233, 1034, 1643, 2558, 2822
MmeI TCCRAC 2 cut(s) 178, 1560
Mox20I TGGCCA 2 cut(s) 88, 1053
Mph1103I ATGCAT 2 cut(s) 304, 2647
MroXI GAANNNNTTC 2 cut(s) 2255, 3126
MscI TGGCCA 2 cut(s) 88, 1053
MslI CAYNNNNRTG 6 cut(s) 753, 1059, 1311, 1728, 1972, 2307
Msp20I TGGCCA 2 cut(s) 88, 1053
MspA1I CMGCKG 5 cut(s) 370, 1628, 2468, 2500, 2947
MspCI CTTAAG 2 cut(s) 1402, 2683
MspI CCGG 3 cut(s) 156, 239, 2392
MspR9I CCNGG 5 cut(s) 157, 894, 1877, 2393, 2810
MunI CAATTG 1 cut(s) 1392
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 3 cut(s) 894, 1877, 2810
MwoI GCNNNNNNNGC 9 cut(s) 94, 109, 620, 629, 923, 1044, 1307, 1763, 2909
NarI GGCGCC 1 cut(s) 1702
NciI CCSGG 2 cut(s) 157, 2393
NdeI CATATG 2 cut(s) 1953, 2139
NlaIV GGNNCC 8 cut(s) 973, 1703, 1889, 1890, 1983, 2781, 3083, 3084
NmeAIII GCCGAG 1 cut(s) 1678
NmuCI GTSAC 5 cut(s) 241, 1105, 2149, 2175, 2225
NsiI ATGCAT 2 cut(s) 304, 2647
NspI RCATGY 4 cut(s) 1042, 1064, 1439, 2680
PaeI GCATGC 1 cut(s) 1042
PagI TCATGA 2 cut(s) 2658, 2827
PaqCI CACCTGC 2 cut(s) 1472, 2480
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PctI GAATGC 1 cut(s) 775
PdmI GAANNNNTTC 2 cut(s) 2255, 3126
PfeI GAWTC 9 cut(s) 25, 127, 496, 581, 1166, 2081, 2108, 2396, 2743
PflFI GACNNNGTC 1 cut(s) 2280
PflMI CCANNNNNTGG 3 cut(s) 908, 1991, 2867
PfoI TCCNGGA 2 cut(s) 155, 2391
PleI GAGTC 5 cut(s) 232, 1033, 1642, 2558, 2821
PluTI GGCGCC 1 cut(s) 1705
PpsI GAGTC 5 cut(s) 232, 1033, 1642, 2558, 2821
PpuMI RGGWCCY 3 cut(s) 35, 1888, 3082
PshBI ATTAAT 1 cut(s) 3099
PsiI TTATAA 1 cut(s) 1871
Psp124BI GAGCTC 1 cut(s) 3003
Psp5II RGGWCCY 3 cut(s) 35, 1888, 3082
Psp6I CCWGG 3 cut(s) 892, 1875, 2808
PspFI CCCAGC 3 cut(s) 521, 2414, 2468
PspGI CCWGG 3 cut(s) 892, 1875, 2808
PspN4I GGNNCC 8 cut(s) 973, 1703, 1889, 1890, 1983, 2781, 3083, 3084
PspPI GGNCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
PspPPI RGGWCCY 3 cut(s) 35, 1888, 3082
PstI CTGCAG 1 cut(s) 1627
PsuI RGATCY 3 cut(s) 2088, 2409, 2960
PsyI GACNNNGTC 1 cut(s) 2280
PvuII CAGCTG 5 cut(s) 370, 1628, 2468, 2500, 2947
RsaI GTAC 2 cut(s) 611, 2878
RsaNI GTAC 2 cut(s) 610, 2877
RseI CAYNNNNRTG 6 cut(s) 753, 1059, 1311, 1728, 1972, 2307
Rsr2I CGGWCCG 1 cut(s) 117
RsrII CGGWCCG 1 cut(s) 117
SacI GAGCTC 1 cut(s) 3003
Sau96I GGNCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
SchI GAGTC 5 cut(s) 233, 1034, 1643, 2558, 2822
ScrFI CCNGG 5 cut(s) 157, 894, 1877, 2393, 2810
SduI GDGCHC 4 cut(s) 439, 832, 2497, 3003
SfaNI GCATC 6 cut(s) 289, 1456, 1587, 2254, 2652, 3074
SfcI CTRYAG 3 cut(s) 687, 921, 1623
SfoI GGCGCC 1 cut(s) 1703
SinI GGWCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
SmiMI CAYNNNNRTG 6 cut(s) 753, 1059, 1311, 1728, 1972, 2307
SmlI CTYRAG 5 cut(s) 71, 1402, 1601, 1858, 2683
SmoI CTYRAG 5 cut(s) 71, 1402, 1601, 1858, 2683
SpeI ACTAGT 1 cut(s) 1786
SphI GCATGC 1 cut(s) 1042
SspDI GGCGCC 1 cut(s) 1701
SstI GAGCTC 1 cut(s) 3003
StyD4I CCNGG 5 cut(s) 155, 892, 1875, 2391, 2808
StyI CCWWGG 5 cut(s) 83, 793, 1536, 1823, 3021
TaaI ACNGT 5 cut(s) 43, 93, 676, 955, 2881
TaiI ACGT 1 cut(s) 2057
TaqI TCGA 6 cut(s) 182, 1850, 2553, 3003, 3029, 3068
TfiI GAWTC 9 cut(s) 25, 127, 496, 581, 1166, 2081, 2108, 2396, 2743
TscAI CASTG 9 cut(s) 567, 1114, 1765, 2073, 2158, 2446, 2736, 2872, 2973
TseFI GTSAC 5 cut(s) 241, 1105, 2149, 2175, 2225
Tsp45I GTSAC 5 cut(s) 241, 1105, 2149, 2175, 2225
TspGWI ACGGA 4 cut(s) 109, 485, 1159, 2792
TspRI CASTG 9 cut(s) 567, 1114, 1765, 2073, 2158, 2446, 2736, 2872, 2973
Tth111I GACNNNGTC 1 cut(s) 2280
Van91I CCANNNNNTGG 3 cut(s) 908, 1991, 2867
Vha464I CTTAAG 2 cut(s) 1402, 2683
VneI GTGCAC 2 cut(s) 828, 2493
VpaK11BI GGWCC 7 cut(s) 35, 117, 1684, 1888, 2523, 2779, 3082
VspI ATTAAT 1 cut(s) 3099
XagI CCTNNNNNAGG 1 cut(s) 532
XapI RAATTY 3 cut(s) 328, 2768, 3013
XbaI TCTAGA 1 cut(s) 2330
XceI RCATGY 4 cut(s) 1042, 1064, 1439, 2680
XmaJI CCTAGG 1 cut(s) 793
XmnI GAANNNNTTC 2 cut(s) 2255, 3126
Zsp2I ATGCAT 2 cut(s) 304, 2647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.