FvH4_7g16270

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0017712
Physical Location & Seq
Reverse (-)
13976743 .. 13977177
435 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16270.t1

Sequence Viewer

Length: 435 bp
ATGCCGAAGAACAAAGGAAAGGGAGGCAAGAACCGCAAGAGGGGAAAGAACGAAGCCGACGACGAGAAGCGAGAGCTCCTCTTCAAAACAGACGGCCAGGAATACGCGCAGGTCACACGCATGTTAGGCAACGGCCGGCTTGAAGCCAGGTGCCACGACGGTGTCACCCGCCTCGGCCACATATCTGGCAAGTTACATAAGAAGGTCTGGATCGCCGCCGGCGATATTGTCCTCATCGGGCTGAGGGACTACCAGGATGGCAAGGCTGATGTGATTCACAAGTACATGCCGGATGAGGCCAGGTTGCTCAAGGCTTATGGGGAGTTGAATGACAGTTTCAGGCCTAATGAGGGCGGCGGCGGCAACAGTGACGACGACATCGACAGCGGCAATGAGTTCCTACAGTTTGAGGATGGTGATGTTGATAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

145

Amino Acids

16.07

Weight (kDa)

6.09

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 32 - 94 7e-24 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017712)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16270
rosa_chinensis RchiOBHm_Chr1g0359281
rosa_laevigata RLG00000027886
rosa_multiflora Rmu_sc0039464.1_g000001 Rmu_ssc0000135.1_g000036 Rmu_ssc0000135.1_g000037
rosa_roxburghii Rroxscaffold_4G00297160
rosa_rugosa Rorug01G0269100 Rorug01G0269200
rosa_samantha Rh1AG283100 Rh1CG267100
rosa_wichuraiana Rw1G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 100
AccB1I GGYRCC 1 cut(s) 150
AccII CGCG 1 cut(s) 107
AciI CCGC 7 cut(s) 34, 169, 216, 354, 357, 360, 387
AclWI GGATC 1 cut(s) 218
AcoI YGGCCR 3 cut(s) 94, 133, 175
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 2 cut(s) 40, 350
AgsI TTSAA 3 cut(s) 85, 143, 328
AjnI CCWGG 4 cut(s) 96, 146, 252, 299
AleI CACNNNNGTG 1 cut(s) 159
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw21I GWGCWC 1 cut(s) 78
AlwI GGATC 1 cut(s) 218
AoxI GGCC 5 cut(s) 94, 133, 175, 297, 341
AspLEI GCGC 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 157, 428
BanI GGYRCC 1 cut(s) 150
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 78
BbvCI CCTCAGC 1 cut(s) 242
BccI CCATC 2 cut(s) 251, 407
BceAI ACGGC 2 cut(s) 109, 148
BciT130I CCWGG 4 cut(s) 98, 148, 254, 301
BfmI CTRYAG 1 cut(s) 401
BfuAI ACCTGC 1 cut(s) 100
BisI GCNGC 5 cut(s) 216, 355, 358, 361, 388
BlsI GCNGC 5 cut(s) 217, 356, 359, 362, 389
Bme1390I CCNGG 4 cut(s) 98, 148, 254, 301
BmiI GGNNCC 1 cut(s) 152
BmrFI CCNGG 4 cut(s) 98, 148, 254, 301
BplI GAGNNNNNCTC 2 cut(s) 63, 95
Bpu10I CCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 172
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 350
Bse118I RCCGGY 2 cut(s) 135, 218
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 4 cut(s) 98, 148, 254, 301
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 3 cut(s) 262, 298, 418
BseLI CCNNNNNNNGG 2 cut(s) 40, 350
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 68
BseX3I CGGCCG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 107
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 5 cut(s) 96, 135, 177, 299, 343
BshNI GGYRCC 1 cut(s) 150
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 3 cut(s) 136, 219, 290
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 40, 350
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 5 cut(s) 96, 135, 177, 299, 343
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 7 cut(s) 34, 169, 216, 354, 357, 360, 387
BspANI GGCC 5 cut(s) 96, 135, 177, 299, 343
BspCNI CTCAG 1 cut(s) 234
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 152
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 397
BsrFI RCCGGY 2 cut(s) 135, 218
BssAI RCCGGY 2 cut(s) 135, 218
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 1 cut(s) 210
Bst2UI CCWGG 4 cut(s) 98, 148, 254, 301
Bst4CI ACNGT 4 cut(s) 161, 335, 368, 405
Bst6I CTCTTC 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 137, 220
BstDEI CTNAG 1 cut(s) 242
BstF5I GGATG 3 cut(s) 262, 298, 418
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 3 cut(s) 33, 126, 360
BstNI CCWGG 4 cut(s) 98, 148, 254, 301
BstNSI RCATGY 2 cut(s) 124, 289
BstSCI CCNGG 4 cut(s) 96, 146, 252, 299
BstSFI CTRYAG 1 cut(s) 401
BstUI CGCG 1 cut(s) 107
BstXI CCANNNNNNTGG 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 133
BsuRI GGCC 5 cut(s) 96, 135, 177, 299, 343
BtsCI GGATG 3 cut(s) 262, 298, 418
BtsIMutI CAGTG 1 cut(s) 373
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 137, 220
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 2 cut(s) 135, 218
Csp6I GTAC 1 cut(s) 283
CviAII CATG 2 cut(s) 121, 286
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
EaeI YGGCCR 3 cut(s) 94, 133, 175
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Ecl136II GAGCTC 1 cut(s) 76
EclXI CGGCCG 1 cut(s) 133
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 4 cut(s) 96, 146, 252, 299
EcoT38I GRGCYC 1 cut(s) 78
FaeI CATG 2 cut(s) 124, 289
FaiI YATR 5 cut(s) 122, 182, 198, 287, 318
FaqI GGGAC 1 cut(s) 260
FatI CATG 2 cut(s) 120, 285
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 5 cut(s) 216, 355, 358, 361, 388
FokI GGATG 3 cut(s) 269, 305, 425
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 5 cut(s) 216, 355, 358, 361, 388
GlaI GCGC 1 cut(s) 108
GluI GCNGC 5 cut(s) 216, 355, 358, 361, 388
HaeIII GGCC 5 cut(s) 96, 135, 177, 299, 343
HapII CCGG 3 cut(s) 136, 219, 290
HhaI GCGC 1 cut(s) 109
Hin1II CATG 2 cut(s) 124, 289
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 2 cut(s) 274, 429
HpaII CCGG 3 cut(s) 136, 219, 290
HphI GGTGA 2 cut(s) 157, 428
Hpy188I TCNGA 1 cut(s) 434
Hpy188III TCNNGA 1 cut(s) 208
Hpy99I CGWCG 4 cut(s) 62, 65, 161, 377
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 4 cut(s) 161, 335, 368, 405
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 126, 360
HpyF3I CTNAG 1 cut(s) 242
Hsp92II CATG 2 cut(s) 124, 289
HspAI GCGC 1 cut(s) 107
KroI GCCGGC 2 cut(s) 135, 218
KroNI GCCGGC 2 cut(s) 137, 220
Kzo9I GATC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 81
MaeIII GTNAC 4 cut(s) 112, 163, 192, 368
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 2 cut(s) 19, 73
MhlI GDGCHC 1 cut(s) 78
MnlI CCTC 9 cut(s) 17, 33, 89, 182, 237, 242, 289, 343, 403
MreI CGCCGGCG 1 cut(s) 218
MroNI GCCGGC 2 cut(s) 135, 218
MslI CAYNNNNRTG 2 cut(s) 119, 159
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 3 cut(s) 136, 219, 290
MspR9I CCNGG 4 cut(s) 98, 148, 254, 301
MvaI CCWGG 4 cut(s) 98, 148, 254, 301
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 3 cut(s) 33, 126, 360
NaeI GCCGGC 2 cut(s) 137, 220
NdeII GATC 1 cut(s) 210
NgoMIV GCCGGC 2 cut(s) 135, 218
NlaIII CATG 2 cut(s) 124, 289
NlaIV GGNNCC 1 cut(s) 152
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 3 cut(s) 112, 163, 368
NspI RCATGY 2 cut(s) 124, 289
OliI CACNNNNGTG 1 cut(s) 159
PceI AGGCCT 1 cut(s) 343
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 219, 378
PdiI GCCGGC 2 cut(s) 137, 220
PfeI GAWTC 2 cut(s) 274, 429
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 5 cut(s) 217, 356, 359, 362, 389
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 4 cut(s) 96, 146, 252, 299
PspGI CCWGG 4 cut(s) 96, 146, 252, 299
PspN4I GGNNCC 1 cut(s) 152
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 2 cut(s) 119, 159
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 5 cut(s) 216, 355, 358, 361, 388
Sau3AI GATC 1 cut(s) 210
ScrFI CCNGG 4 cut(s) 98, 148, 254, 301
SduI GDGCHC 1 cut(s) 78
SetI ASST 5 cut(s) 78, 114, 152, 207, 305
SfcI CTRYAG 1 cut(s) 401
SgrAI CRCCGGYG 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 119, 159
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 7 cut(s) 34, 169, 216, 354, 357, 360, 387
SstI GAGCTC 1 cut(s) 78
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 4 cut(s) 96, 146, 252, 299
TaaI ACNGT 4 cut(s) 161, 335, 368, 405
TaqI TCGA 1 cut(s) 381
TatI WGTACW 1 cut(s) 282
TauI GCSGC 5 cut(s) 218, 357, 360, 363, 390
TfiI GAWTC 2 cut(s) 274, 429
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 3 cut(s) 112, 163, 368
Tsp45I GTSAC 3 cut(s) 112, 163, 368
TspRI CASTG 1 cut(s) 373
Tth111I GACNNNGTC 1 cut(s) 161
XceI RCATGY 2 cut(s) 124, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.