Rorug01G0269100

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017712
Physical Location & Seq
Reverse (-)
38155810 .. 38159004
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0269100.1

Sequence Viewer

Length: 3195 bp
ATGAAAACTTTGCTACATTTCTTCCTTCTCTTGATCACACTCTGGGTCAGTATCATCATCCCAGCAGTTCACAGCCAGTGTATTAAAGACCAGCAACTATCATTGCTCCAATTCAAGAAAAACCTCGTGTTCGAGGGTCCTTCCACCAAGCTTATTTCATGGAATGCAAGGACTGATTGTTGCTCTTGGGTGGGCGTCACTTGCAGCCTTAATGGGCGTGTTCTGGGTCTTGACATAAGCAGCGAAGGTATCTCAGCTGGAATTGACAATTCCAGCAGTCTCTTCCATCTTCATTATCTTCAAAGCCTCAATTTGGCGCACAACCAACTTGGCAGTATCTCTCAATCAATTCCATCCGCAATCGGAAATCTCCTGAACTTGAGGTATCTGAATTTATCTCTTACTGATTATTCAGGGCAAATTCCCATCGAGATTTCACGCTTGAGGAGATTGGTAGTTCTTGATTTTTCTAGTTATTATCCGGCCGACTTAAAACTTGGAAGTCCAAATTTACACACGTTTGTTCAAAACTTCACAGAGCTTAGAGAGTTGTATCTTGATTGGGTCCAAATATCAGAACAGGGGAGGGAGTGGTGCCGAGCCATATCAGATTCCCTTCCTAACCTCAGGGTCTTGAGCATGTCCTCGTGTGATCTCTCAGGCCCTTTTCTCCAGTCCCTTGCTAAGCTTCGGTCTCTATCCGTGATTAGGTTGGACTTCAACGATATCTCTGCGCCAGTTCCAGGATTCTTTGCCAACTTTTCAAACTTGACTGTCTTGAGTCTCCAGGGATGCGAGTTGCATGGAACATTTCCCAAAGAGATCTTTCAGTTACCTTCTCTACAAAGTATTGACCTTTCATTTAATGACCAACTTGATGGTTCCTTGCCAGAATTCTCAAGCAATGGATCTCTTCAGTACCTGCATCTAAGCCTGACAAACTTTTCAGGGTTATTGCCAAATTCTATTGGCAATCTCAAAATGTTGTCCGGCATAAGTATTGGAGAATGCAATTTCACGGGACCGATCCCCAAGTCAATGACAAACCTTACACAGTTGGTTTATTTGTTGATGCCCAATAACATGTTGGAAGGTTCAATTCCGTCATTCAGTGGGGCCAAGAATGTTGTCACCATACTCCTTTACAACAATGGTCTAACAGGTACTATTAATTCCACCCACTGGAGAAACCTTACTAAGCTATTCACTCTCTATTTGGGATCCAATAAGCTCGATGGGAATATCCCAGCATCTCTGTTTTCTCTTCCCCTGTTGGATCGTCTAGATCTTTCCAGAAATCAATTCTCTGGTCCATTCCCTGAAATTTCTAATGTGTCCTCCTCCTTGCTGAATGGTCCTGATCTTGCAATTTATTTGGGTAGCAACAATCTCGAAGGGCCAATACCTATGTCTATCTTTAGCTTGCGACGTCTTTGGGTTCTAGATCTTTCTTCAAATAATTTAAGTGGCTCGTTTCCTCTTGATGGTCTACACCAGCTGAGAAATCTTTCTCTACTTGATCTTTCACACAATAACTTGTTTCTTACCCATGATTATACCAATTTCTCATATTCCTCATTTTCTCAATTATCTACTCTGAGATTGGTGTCATTGAAATTAAGAACATTTCCTAATTTCTTGAGAAATCAATCTAACTTGCAAACTTTGGACCTTTCAGATAACCAGATAAATGGCAAGATACCCAACTGGATTTGGAGTCTTAGTGATCTTACTTACCTAAATCTTTCTTGCAACTCCCTAGATTCTCTAGAAGTTCCTTCAAACAATCTCAGTAAATTATGGTACCTTGACCTCCATTCCAACCAGTTTCATGGGCAAATCCCAATTTTTCCATCACCTTACAGTTTCGATTATCTAGATTTCTCGAGAAATTATTTCAGCTCTCCCATACCGAGTACCACTGGAGATATGCTTGCATACACTGGATTCTTGTCGCTTTCGAGCAATAACCTCAATGGGATCATTCCAGAATCATTATGCAATTCACAATTTCTTCAGATTCTTGATCTGTCCAATAATTCTTTGAGTGGCACGGTTCCCCGGTGCTTGACTACAATGAGCACGCTTGAAGTACTCAATTTAAGGAGAAACAATCTTACAAATGTTAATGAATTCTCTCAACATTGCAGTTTAGAAACGCTAGACATCAGTGGAAATCAAATTCAAGGCCAGTTTTTAAAATCTCTTGTACACTGCACCCAGTTAACGGTTTTAAATCTTGGACACAATCTTATAGCAGAACCATTTCCATGCTTTTTGAGGAACACATCCACCTTGCGTGTCCTTGTCTTGCGATCCAATAAATTCTATGGGCGCATTAGATGTTGCAAGACTAATGGCACTTGGCCAGTACTTCAAATCATAGACTTAGCTCACAACAATTTGAGTGGTGAAGTACCTGGAACAGCTTTGACAACTTGGCAGGCGATGAGGACTAGCAAAGATAATGCCCCATGGAAGATCAGTGACCTTCAATTTAGATACAGTGGAGCTTATTATAAAGAAGATACAGTCATGGTTAGCAACAAAGGTTTAGAGATGGAGCTGGTCAATATTTTAACAATCTTCACCTCCATTGACTTCTCATGCAACAAGTTCAACGGATCAATACCGGAGGAAATTGGAGAACTCAAATCATTATATGCCCTCAACTTGTCCAACAATGCTTTCACAGGTGCAGTTCCATCATCATTAAGTAACTTGAGTGAGCTAGAGTCTTTGGACCTCTCGAGAAACAAACTGAGCGGTCAAATCCCACCGGAGCTTACAAAACTCACTTTCCTTGAATTCTTGAATCTCTCATATAATCAATTGGTCGGGAGGATACCAAGCGGTGCTCAATTTTCAACATTTGATGCAGCTTCCTTCCAAGGTAACAAAGGGTTGTGGGGGCCTCCTTTAACAACATATAATGGAACAGGGTTCTCACCAAAGTTGGAAGGAAATCATTCAAATCCTGGACATGAGATTGATTGGGATATTATCTGTCCTGAAATTGGATTTACATGTGGGTTTGGGATTGTCATCGGGTCACTTCTGTTTTGCAAGAGATGGAGGAAATGGTATTACAGAGCTATGTATAACATGCTTCTAAAGATATTCCCTCAGCTGGAACAGAGATTTGGTCATCACAGGAGACATGTTTATGCACATCAAAGATTCTGGAGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

1064

Amino Acids

119.17

Weight (kDa)

6.92

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 69 9.8e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 73 - 288 1.9e-12 Leucine-rich repeat region
LRR_8 PF13855 231 - 290 3.4e-07 Leucine rich repeat
LRR_14 PF23598 304 - 362 5.9e-06 Leucine-rich repeat region
LRR_14 PF23598 390 - 632 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 533 - 587 2.7e-06 Leucine rich repeat
LRR_8 PF13855 551 - 610 1.4e-07 Leucine rich repeat
LRR_8 PF13855 672 - 728 3.6e-09 Leucine rich repeat
LRR_8 PF13855 695 - 752 6e-06 Leucine rich repeat
LRR_8 PF13855 886 - 945 1.2e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017712)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16270
rosa_chinensis RchiOBHm_Chr1g0359281
rosa_laevigata RLG00000027886
rosa_multiflora Rmu_sc0039464.1_g000001 Rmu_ssc0000135.1_g000036 Rmu_ssc0000135.1_g000037
rosa_roxburghii Rroxscaffold_4G00297160
rosa_rugosa Rorug01G0269100 Rorug01G0269200
rosa_samantha Rh1AG283100 Rh1CG267100
rosa_wichuraiana Rw1G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2520
AatII GACGTC 1 cut(s) 1432
Acc36I ACCTGC 1 cut(s) 930
Acc65I GGTACC 1 cut(s) 1803
AccB1I GGYRCC 2 cut(s) 594, 1803
AccB7I CCANNNNNTGG 1 cut(s) 1182
AccBSI CCGCTC 1 cut(s) 2766
AccI GTMKAC 1 cut(s) 1489
AciI CCGC 3 cut(s) 357, 2766, 2853
AclWI GGATC 8 cut(s) 916, 1021, 1215, 1228, 1284, 1988, 2310, 2632
AcoI YGGCCR 2 cut(s) 483, 2366
AcuI CTGAAG 2 cut(s) 899, 2000
AcyI GRCGYC 2 cut(s) 195, 1429
AfaI GTAC 8 cut(s) 920, 1165, 1805, 1918, 2094, 2211, 2373, 2418
AfiI CCNNNNNNNGG 8 cut(s) 313, 708, 743, 1182, 1484, 2227, 3017, 3130
AflIII ACRYGT 4 cut(s) 516, 1083, 3026, 3160
AjnI CCWGG 4 cut(s) 742, 786, 2419, 2977
AjuI GAANNNNNNNTTGG 4 cut(s) 2672, 2704, 3126, 3158
Alw21I GWGCWC 2 cut(s) 2084, 2860
Alw26I GTCTC 5 cut(s) 284, 699, 788, 3151, 3181
AlwI GGATC 8 cut(s) 916, 1021, 1215, 1228, 1284, 1988, 2310, 2632
AlwNI CAGNNNCTG 1 cut(s) 922
Ama87I CYCGRG 2 cut(s) 1885, 2749
AoxI GGCC 7 cut(s) 483, 661, 1116, 1397, 2188, 2366, 2912
ApeKI GCWGC 3 cut(s) 204, 240, 2879
AseI ATTAAT 1 cut(s) 1170
Asp700I GAANNNNTTC 3 cut(s) 1507, 2265, 2968
Asp718I GGTACC 1 cut(s) 1803
AspLEI GCGC 3 cut(s) 319, 736, 2337
AsuC2I CCSGG 1 cut(s) 2062
AsuHPI GGTGA 5 cut(s) 1123, 1848, 2423, 2581, 2940
AvaI CYCGRG 2 cut(s) 1885, 2749
AvaII GGWCC 7 cut(s) 137, 565, 1022, 1310, 1355, 1669, 2743
AxyI CCTNAGG 1 cut(s) 626
BalI TGGCCA 1 cut(s) 2368
BamHI GGATCC 1 cut(s) 1220
BanI GGYRCC 2 cut(s) 594, 1803
BarI GAAGNNNNNNTAC 2 cut(s) 1386, 1418
BauI CACGAG 2 cut(s) 125, 646
Bbv12I GWGCWC 2 cut(s) 2084, 2860
BbvCI CCTCAGC 1 cut(s) 3126
BbvI GCAGC 3 cut(s) 216, 252, 2891
BcgI CGANNNNNNTGC 4 cut(s) 713, 747, 1372, 1406
BciT130I CCWGG 4 cut(s) 744, 788, 2421, 2979
BciVI GTATCC 1 cut(s) 2838
BclI TGATCA 1 cut(s) 33
BcnI CCSGG 1 cut(s) 2062
BcoDI GTCTC 5 cut(s) 284, 699, 788, 3151, 3181
BfaI CTAG 9 cut(s) 471, 1283, 1442, 1760, 1769, 1877, 2162, 2457, 2732
BfuAI ACCTGC 1 cut(s) 930
BfuI GTATCC 1 cut(s) 2838
BglII AGATCT 3 cut(s) 822, 1285, 1444
BisI GCNGC 3 cut(s) 205, 241, 2880
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 3 cut(s) 206, 242, 2881
BmcAI AGTACT 2 cut(s) 2094, 2373
Bme1390I CCNGG 5 cut(s) 744, 788, 2062, 2421, 2979
Bme18I GGWCC 7 cut(s) 137, 565, 1022, 1310, 1355, 1669, 2743
BmeT110I CYCGRG 2 cut(s) 1885, 2749
BmrFI CCNGG 5 cut(s) 744, 788, 2062, 2421, 2979
BmrI ACTGGG 1 cut(s) 2215
BmsI GCATC 5 cut(s) 782, 934, 1062, 1259, 2866
BmuI ACTGGG 1 cut(s) 2215
BpmI CTGGAG 4 cut(s) 656, 770, 1204, 1944
Bpu10I CCTNAGC 1 cut(s) 3126
Bpu1102I GCTNAGC 1 cut(s) 684
BpuEI CTTGAG 7 cut(s) 400, 463, 655, 799, 883, 1660, 2743
BpuMI CCSGG 1 cut(s) 2062
BsaBI GATNNNNATC 1 cut(s) 3044
BsaHI GRCGYC 2 cut(s) 195, 1429
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 4 cut(s) 787, 2060, 2474, 2890
BsaWI WCCGGW 2 cut(s) 2632, 2779
BsaXI ACNNNNNCTCC 2 cut(s) 1708, 1738
Bsc4I CCNNNNNNNGG 8 cut(s) 313, 708, 743, 1182, 1484, 2227, 3017, 3130
Bse21I CCTNAGG 1 cut(s) 626
Bse3DI GCAATG 3 cut(s) 101, 910, 2143
Bse8I GATNNNNATC 1 cut(s) 3044
BseBI CCWGG 4 cut(s) 744, 788, 2421, 2979
BseDI CCNNGG 4 cut(s) 787, 2060, 2474, 2890
BseGI GGATG 4 cut(s) 57, 353, 797, 2288
BseJI GATNNNNATC 1 cut(s) 3044
BseLI CCNNNNNNNGG 8 cut(s) 313, 708, 743, 1182, 1484, 2227, 3017, 3130
BseMI GCAATG 3 cut(s) 101, 910, 2143
BseMII CTCAG 8 cut(s) 267, 640, 672, 1490, 1589, 1804, 2753, 3140
BseRI GAGGAG 2 cut(s) 460, 1330
BseX3I CGGCCG 1 cut(s) 483
BseXI GCAGC 3 cut(s) 216, 252, 2891
BseYI CCCAGC 2 cut(s) 61, 1246
BsgI GTGCAG 2 cut(s) 2200, 2718
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 7 cut(s) 485, 663, 1118, 1399, 2190, 2368, 2914
BshNI GGYRCC 2 cut(s) 594, 1803
BsiEI CGRYCG 1 cut(s) 486
BsiHKAI GWGCWC 2 cut(s) 2084, 2860
BsiHKCI CYCGRG 2 cut(s) 1885, 2749
BsiSI CCGG 5 cut(s) 482, 990, 2062, 2633, 2780
BslFI GGGAC 2 cut(s) 661, 1035
BslI CCNNNNNNNGG 8 cut(s) 313, 708, 743, 1182, 1484, 2227, 3017, 3130
BsmAI GTCTC 5 cut(s) 284, 699, 788, 3151, 3181
BsmBI CGTCTC 1 cut(s) 3181
BsmFI GGGAC 2 cut(s) 661, 1035
BsmI GAATGC 2 cut(s) 169, 1013
BsnI GGCC 7 cut(s) 485, 663, 1118, 1399, 2190, 2368, 2914
Bso31I GGTCTC 1 cut(s) 699
BsoBI CYCGRG 2 cut(s) 1885, 2749
Bsp1286I GDGCHC 2 cut(s) 2084, 2860
Bsp1407I TGTACA 1 cut(s) 2209
Bsp1720I GCTNAGC 1 cut(s) 684
Bsp19I CCATGG 1 cut(s) 2474
BspACI CCGC 3 cut(s) 357, 2766, 2853
BspANI GGCC 7 cut(s) 485, 663, 1118, 1399, 2190, 2368, 2914
BspCNI CTCAG 8 cut(s) 266, 639, 671, 1491, 1590, 1803, 2754, 3139
BspMI ACCTGC 1 cut(s) 930
BspPI GGATC 8 cut(s) 916, 1021, 1215, 1228, 1284, 1988, 2310, 2632
BspT107I GGYRCC 2 cut(s) 594, 1803
BspTNI GGTCTC 1 cut(s) 699
BsrBI CCGCTC 1 cut(s) 2766
BsrDI GCAATG 3 cut(s) 101, 910, 2143
BsrGI TGTACA 1 cut(s) 2209
BssECI CCNNGG 4 cut(s) 787, 2060, 2474, 2890
BssNI GRCGYC 2 cut(s) 195, 1429
BssSI CACGAG 2 cut(s) 125, 646
BssT1I CCWWGG 2 cut(s) 2474, 2890
Bst2BI CACGAG 2 cut(s) 125, 646
Bst2UI CCWGG 4 cut(s) 744, 788, 2421, 2979
Bst4CI ACNGT 7 cut(s) 775, 1056, 1865, 2056, 2230, 2507, 2533
Bst6I CTCTTC 3 cut(s) 287, 918, 1269
BstACI GRCGYC 2 cut(s) 195, 1429
BstAUI TGTACA 1 cut(s) 2209
BstC8I GCNNGC 4 cut(s) 1424, 1935, 2084, 2445
BstDSI CCRYGG 1 cut(s) 2474
BstF5I GGATG 4 cut(s) 57, 353, 797, 2288
BstHHI GCGC 3 cut(s) 319, 736, 2337
BstMAI GTCTC 5 cut(s) 284, 699, 788, 3151, 3181
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstNI CCWGG 4 cut(s) 744, 788, 2421, 2979
BstNSI RCATGY 5 cut(s) 643, 1087, 3030, 3109, 3164
BstSCI CCNGG 5 cut(s) 742, 786, 2060, 2419, 2977
BstV1I GCAGC 3 cut(s) 216, 252, 2891
BstX2I RGATCY 5 cut(s) 822, 908, 1220, 1285, 1444
BstXI CCANNNNNNTGG 3 cut(s) 878, 1691, 1832
BstYI RGATCY 5 cut(s) 822, 908, 1220, 1285, 1444
BstZI CGGCCG 1 cut(s) 483
Bsu36I CCTNAGG 1 cut(s) 626
BsuI GTATCC 1 cut(s) 2838
BsuRI GGCC 7 cut(s) 485, 663, 1118, 1399, 2190, 2368, 2914
BtgI CCRYGG 1 cut(s) 2474
BtgZI GCGATG 1 cut(s) 2462
BtsCI GGATG 4 cut(s) 57, 353, 797, 2288
BtsI GCAGTG 1 cut(s) 2212
BtsIMutI CAGTG 9 cut(s) 83, 1117, 1180, 1920, 1941, 2176, 2212, 2491, 2512
BveI ACCTGC 1 cut(s) 930
Cac8I GCNNGC 4 cut(s) 1424, 1935, 2084, 2445
CaiI CAGNNNCTG 1 cut(s) 922
CfoI GCGC 3 cut(s) 319, 736, 2337
CseI GACGC 1 cut(s) 184
Csp6I GTAC 8 cut(s) 919, 1164, 1804, 1917, 2093, 2210, 2372, 2417
CspCI CAANNNNNGTGG 2 cut(s) 2389, 2424
CviQI GTAC 8 cut(s) 919, 1164, 1804, 1917, 2093, 2210, 2372, 2417
DraI TTTAAA 2 cut(s) 2199, 2235
EaeI YGGCCR 2 cut(s) 483, 2366
EagI CGGCCG 1 cut(s) 483
Eam1104I CTCTTC 3 cut(s) 287, 918, 1269
EarI CTCTTC 3 cut(s) 287, 918, 1269
EclXI CGGCCG 1 cut(s) 483
Eco130I CCWWGG 2 cut(s) 2474, 2890
Eco31I GGTCTC 1 cut(s) 699
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 7 cut(s) 137, 565, 1022, 1310, 1355, 1669, 2743
Eco52I CGGCCG 1 cut(s) 483
Eco57I CTGAAG 2 cut(s) 899, 2000
Eco81I CCTNAGG 1 cut(s) 626
Eco88I CYCGRG 2 cut(s) 1885, 2749
EcoO109I RGGNCCY 3 cut(s) 137, 662, 2912
EcoRI GAATTC 3 cut(s) 893, 2132, 2807
EcoRII CCWGG 4 cut(s) 742, 786, 2419, 2977
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 2 cut(s) 2474, 2890
ErhI CCWWGG 2 cut(s) 2474, 2890
Esp3I CGTCTC 1 cut(s) 3181
FaqI GGGAC 2 cut(s) 661, 1035
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 1 cut(s) 1489
Fnu4HI GCNGC 3 cut(s) 205, 241, 2880
FokI GGATG 4 cut(s) 44, 340, 804, 2275
Fsp4HI GCNGC 3 cut(s) 205, 241, 2880
FspBI CTAG 9 cut(s) 471, 1283, 1442, 1760, 1769, 1877, 2162, 2457, 2732
GlaI GCGC 3 cut(s) 318, 735, 2336
GluI GCNGC 3 cut(s) 205, 241, 2880
GsaI CCCAGC 2 cut(s) 65, 1250
GsuI CTGGAG 4 cut(s) 656, 770, 1204, 1944
HaeIII GGCC 7 cut(s) 485, 663, 1118, 1399, 2190, 2368, 2914
HapII CCGG 5 cut(s) 482, 990, 2062, 2633, 2780
HgaI GACGC 1 cut(s) 184
HhaI GCGC 3 cut(s) 319, 736, 2337
Hin1I GRCGYC 2 cut(s) 195, 1429
Hin6I GCGC 3 cut(s) 317, 734, 2335
HinP1I GCGC 3 cut(s) 317, 734, 2335
HincII GTYRAC 1 cut(s) 2226
HindII GTYRAC 1 cut(s) 2226
HindIII AAGCTT 2 cut(s) 149, 686
HpaI GTTAAC 1 cut(s) 2226
HpaII CCGG 5 cut(s) 482, 990, 2062, 2633, 2780
HphI GGTGA 5 cut(s) 1123, 1848, 2423, 2581, 2940
Hpy166II GTNNAC 4 cut(s) 70, 1490, 2212, 2226
Hpy188I TCNGA 7 cut(s) 365, 390, 577, 610, 1599, 1678, 2019
Hpy8I GTNNAC 4 cut(s) 70, 1490, 2212, 2226
Hpy99I CGWCG 1 cut(s) 1431
HpyCH4III ACNGT 7 cut(s) 775, 1056, 1865, 2056, 2230, 2507, 2533
HpyCH4IV ACGT 3 cut(s) 518, 1429, 3190
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
HpySE526I ACGT 3 cut(s) 518, 1429, 3190
Hsp92I GRCGYC 2 cut(s) 195, 1429
HspAI GCGC 3 cut(s) 317, 734, 2335
KpnI GGTACC 1 cut(s) 1807
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 2226
LmnI GCTCC 4 cut(s) 111, 2510, 2563, 2782
Lsp1109I GCAGC 3 cut(s) 216, 252, 2891
LweI GCATC 5 cut(s) 782, 934, 1062, 1259, 2866
MaeI CTAG 9 cut(s) 471, 1283, 1442, 1760, 1769, 1877, 2162, 2457, 2732
MaeII ACGT 3 cut(s) 518, 1429, 3190
MaeIII GTNAC 7 cut(s) 196, 831, 1129, 2486, 2717, 2894, 3051
MbiI CCGCTC 1 cut(s) 2766
MfeI CAATTG 1 cut(s) 2831
MflI RGATCY 5 cut(s) 822, 908, 1220, 1285, 1444
MhlI GDGCHC 2 cut(s) 2084, 2860
MlsI TGGCCA 1 cut(s) 2368
MluNI TGGCCA 1 cut(s) 2368
MlyI GAGTC 3 cut(s) 790, 1726, 2744
MmeI TCCRAC 6 cut(s) 693, 1068, 1254, 1845, 2703, 2937
Mox20I TGGCCA 1 cut(s) 2368
MroXI GAANNNNTTC 3 cut(s) 1507, 2265, 2968
MscI TGGCCA 1 cut(s) 2368
MslI CAYNNNNRTG 2 cut(s) 2269, 3165
Msp20I TGGCCA 1 cut(s) 2368
MspA1I CMGCKG 3 cut(s) 257, 1498, 3130
MspI CCGG 5 cut(s) 482, 990, 2062, 2633, 2780
MspR9I CCNGG 5 cut(s) 744, 788, 2062, 2421, 2979
MunI CAATTG 1 cut(s) 2831
Mva1269I GAATGC 2 cut(s) 169, 1013
MvaI CCWGG 4 cut(s) 744, 788, 2421, 2979
MwoI GCNNNNNNNGC 1 cut(s) 201
NciI CCSGG 1 cut(s) 2062
NcoI CCATGG 1 cut(s) 2474
NmeAIII GCCGAG 1 cut(s) 623
NmuCI GTSAC 4 cut(s) 196, 1129, 2486, 3051
NspI RCATGY 5 cut(s) 643, 1087, 3030, 3109, 3164
PaeR7I CTCGAG 2 cut(s) 1885, 2749
PciI ACATGT 3 cut(s) 1083, 3026, 3160
PctI GAATGC 2 cut(s) 169, 1013
PdmI GAANNNNTTC 3 cut(s) 1507, 2265, 2968
PfeI GAWTC 8 cut(s) 611, 747, 1763, 1947, 1991, 2020, 2815, 3180
PflMI CCANNNNNTGG 1 cut(s) 1182
PfoI TCCNGGA 2 cut(s) 742, 2977
PkrI GCNGC 3 cut(s) 206, 242, 2881
PleI GAGTC 3 cut(s) 789, 1725, 2743
PpsI GAGTC 3 cut(s) 789, 1725, 2743
PpuMI RGGWCCY 1 cut(s) 137
PscI ACATGT 3 cut(s) 1083, 3026, 3160
PshBI ATTAAT 1 cut(s) 1170
PsiI TTATAA 1 cut(s) 2520
Psp5II RGGWCCY 1 cut(s) 137
Psp6I CCWGG 4 cut(s) 742, 786, 2419, 2977
PspFI CCCAGC 2 cut(s) 61, 1246
PspGI CCWGG 4 cut(s) 742, 786, 2419, 2977
PspPPI RGGWCCY 1 cut(s) 137
PstNI CAGNNNCTG 1 cut(s) 922
PsuI RGATCY 5 cut(s) 822, 908, 1220, 1285, 1444
PvuII CAGCTG 3 cut(s) 257, 1498, 3130
RsaI GTAC 8 cut(s) 920, 1165, 1805, 1918, 2094, 2211, 2373, 2418
RsaNI GTAC 8 cut(s) 919, 1164, 1804, 1917, 2093, 2210, 2372, 2417
RseI CAYNNNNRTG 2 cut(s) 2269, 3165
SatI GCNGC 3 cut(s) 205, 241, 2880
ScaI AGTACT 2 cut(s) 2094, 2373
SchI GAGTC 3 cut(s) 790, 1726, 2744
ScrFI CCNGG 5 cut(s) 744, 788, 2062, 2421, 2979
SduI GDGCHC 2 cut(s) 2084, 2860
SfaNI GCATC 5 cut(s) 782, 934, 1062, 1259, 2866
Sfr274I CTCGAG 2 cut(s) 1885, 2749
SinI GGWCC 7 cut(s) 137, 565, 1022, 1310, 1355, 1669, 2743
SlaI CTCGAG 2 cut(s) 1885, 2749
SmiMI CAYNNNNRTG 2 cut(s) 2269, 3165
SmlI CTYRAG 9 cut(s) 379, 442, 634, 778, 898, 1639, 1885, 2722, 2749
SmoI CTYRAG 9 cut(s) 379, 442, 634, 778, 898, 1639, 1885, 2722, 2749
SsiI CCGC 3 cut(s) 357, 2766, 2853
SspI AATATT 1 cut(s) 2575
SspMI CTAG 9 cut(s) 471, 1283, 1442, 1760, 1769, 1877, 2162, 2457, 2732
StyD4I CCNGG 5 cut(s) 742, 786, 2060, 2419, 2977
StyI CCWWGG 2 cut(s) 2474, 2890
TaaI ACNGT 7 cut(s) 775, 1056, 1865, 2056, 2230, 2507, 2533
TaiI ACGT 3 cut(s) 521, 1432, 3193
TaqI TCGA 8 cut(s) 132, 429, 1233, 1392, 1869, 1886, 1961, 2750
TaqII GACCGA 2 cut(s) 681, 1039
TatI WGTACW 3 cut(s) 2092, 2209, 2371
TfiI GAWTC 8 cut(s) 611, 747, 1763, 1947, 1991, 2020, 2815, 3180
TscAI CASTG 9 cut(s) 83, 1117, 1187, 1927, 1948, 2176, 2219, 2491, 2512
TseFI GTSAC 4 cut(s) 196, 1129, 2486, 3051
TseI GCWGC 3 cut(s) 204, 240, 2879
Tsp45I GTSAC 4 cut(s) 196, 1129, 2486, 3051
TspDTI ATGAA 6 cut(s) 17, 147, 281, 849, 1820, 2145
TspGWI ACGGA 3 cut(s) 691, 1092, 2637
TspRI CASTG 9 cut(s) 83, 1117, 1187, 1927, 1948, 2176, 2219, 2491, 2512
Van91I CCANNNNNTGG 1 cut(s) 1182
VpaK11BI GGWCC 7 cut(s) 137, 565, 1022, 1310, 1355, 1669, 2743
VspI ATTAAT 1 cut(s) 1170
XbaI TCTAGA 4 cut(s) 1282, 1441, 1768, 1876
XceI RCATGY 5 cut(s) 643, 1087, 3030, 3109, 3164
XcmI CCANNNNNNNNNTGG 1 cut(s) 1084
XhoI CTCGAG 2 cut(s) 1885, 2749
XmiI GTMKAC 1 cut(s) 1489
XmnI GAANNNNTTC 3 cut(s) 1507, 2265, 2968
XspI CTAG 9 cut(s) 471, 1283, 1442, 1760, 1769, 1877, 2162, 2457, 2732
ZraI GACGTC 1 cut(s) 1430
ZrmI AGTACT 2 cut(s) 2094, 2373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.