Rmu_ssc0000135.1_g000036

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000135.1
OG0017712
Physical Location & Seq
Reverse (-)
185480 .. 185908
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000135.1_g000036.1.cds

Sequence Viewer

Length: 429 bp
atgccgaagaacaaaggaaagggaggcaagaaccggaagagagggaaaaacgaagccgacgacgagaagcgcgagctcctcttcaaagaggacggccaggaatacgcgcaggtcacacgcatgttaggcaacggccggttggaagccaaatgccacgacggcgtcgcccgcctctgccacatctccggcaagctacacaagaaggtctggatcgccgccggcgatattctccttgtcgggctaagggactaccaggatggcaaggctgatgtcatccacaagtacatgcccgacgaggcacggttgctcaaggcttatggggagttgaatgacagtttcaggccgaatgagggcggcaatgatgacgacgacgacaacaatggcgatggttacatagagtttgaggatgctgatgttgataaaatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

142

Amino Acids

15.96

Weight (kDa)

5.41

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017712)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g16270
rosa_chinensis RchiOBHm_Chr1g0359281
rosa_laevigata RLG00000027886
rosa_multiflora Rmu_sc0039464.1_g000001 Rmu_ssc0000135.1_g000036 Rmu_ssc0000135.1_g000037
rosa_roxburghii Rroxscaffold_4G00297160
rosa_rugosa Rorug01G0269100 Rorug01G0269200
rosa_samantha Rh1AG283100 Rh1CG267100
rosa_wichuraiana Rw1G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 100
AccII CGCG 2 cut(s) 72, 107
AciI CCGC 3 cut(s) 169, 216, 354
AclWI GGATC 1 cut(s) 218
AcoI YGGCCR 2 cut(s) 94, 133
AcsI RAATTY 1 cut(s) 423
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 350
AgsI TTSAA 2 cut(s) 85, 328
AjnI CCWGG 2 cut(s) 96, 252
AluBI AGCT 2 cut(s) 76, 193
AluI AGCT 2 cut(s) 76, 193
Alw21I GWGCWC 1 cut(s) 78
AlwI GGATC 1 cut(s) 218
AoxI GGCC 3 cut(s) 94, 133, 341
ApoI RAATTY 1 cut(s) 423
AspLEI GCGC 2 cut(s) 72, 109
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 78
BccI CCATC 2 cut(s) 251, 380
BceAI ACGGC 3 cut(s) 109, 148, 175
BciT130I CCWGG 2 cut(s) 98, 254
BfuAI ACCTGC 1 cut(s) 100
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 2 cut(s) 216, 355
BlsI GCNGC 2 cut(s) 217, 356
Bme1390I CCNGG 2 cut(s) 98, 254
BmrFI CCNGG 2 cut(s) 98, 254
BmsI GCATC 1 cut(s) 397
Bpu10I CCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse118I RCCGGY 2 cut(s) 135, 218
Bse3DI GCAATG 1 cut(s) 364
BseBI CCWGG 2 cut(s) 98, 254
BseGI GGATG 3 cut(s) 262, 273, 412
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMI GCAATG 1 cut(s) 364
BseRI GAGGAG 1 cut(s) 68
BseX3I CGGCCG 1 cut(s) 133
Bsh1236I CGCG 2 cut(s) 72, 107
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 3 cut(s) 96, 135, 343
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 4 cut(s) 34, 136, 186, 219
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 3 cut(s) 96, 135, 343
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 3 cut(s) 169, 216, 354
BspANI GGCC 3 cut(s) 96, 135, 343
BspFNI CGCG 2 cut(s) 72, 107
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 364
BsrFI RCCGGY 2 cut(s) 135, 218
BssAI RCCGGY 2 cut(s) 135, 218
BssMI GATC 1 cut(s) 210
BssNI GRCGYC 1 cut(s) 162
Bst2UI CCWGG 2 cut(s) 98, 254
Bst4CI ACNGT 2 cut(s) 303, 335
Bst6I CTCTTC 2 cut(s) 32, 86
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 4 cut(s) 74, 169, 191, 220
BstDEI CTNAG 1 cut(s) 242
BstF5I GGATG 3 cut(s) 262, 273, 412
BstFNI CGCG 2 cut(s) 72, 107
BstHHI GCGC 2 cut(s) 72, 109
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 3 cut(s) 126, 159, 168
BstNI CCWGG 2 cut(s) 98, 254
BstNSI RCATGY 2 cut(s) 124, 289
BstSCI CCNGG 2 cut(s) 96, 252
BstUI CGCG 2 cut(s) 72, 107
BstZI CGGCCG 1 cut(s) 133
BsuRI GGCC 3 cut(s) 96, 135, 343
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 3 cut(s) 262, 273, 412
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 4 cut(s) 74, 169, 191, 220
CfoI GCGC 2 cut(s) 72, 109
Cfr10I RCCGGY 2 cut(s) 135, 218
CseI GACGC 1 cut(s) 151
Csp6I GTAC 1 cut(s) 283
CviAII CATG 2 cut(s) 121, 286
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
EaeI YGGCCR 2 cut(s) 94, 133
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 32, 86
EarI CTCTTC 2 cut(s) 32, 86
Ecl136II GAGCTC 1 cut(s) 76
EclXI CGGCCG 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 2 cut(s) 96, 252
EcoT38I GRGCYC 1 cut(s) 78
FaeI CATG 2 cut(s) 124, 289
FaiI YATR 4 cut(s) 122, 287, 318, 395
FaqI GGGAC 1 cut(s) 260
FatI CATG 2 cut(s) 120, 285
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 216, 355
FokI GGATG 3 cut(s) 260, 269, 419
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 2 cut(s) 216, 355
GlaI GCGC 2 cut(s) 71, 108
GluI GCNGC 2 cut(s) 216, 355
HaeIII GGCC 3 cut(s) 96, 135, 343
HapII CCGG 4 cut(s) 34, 136, 186, 219
HgaI GACGC 1 cut(s) 151
HhaI GCGC 2 cut(s) 72, 109
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 2 cut(s) 124, 289
Hin6I GCGC 2 cut(s) 70, 107
HinP1I GCGC 2 cut(s) 70, 107
HpaII CCGG 4 cut(s) 34, 136, 186, 219
Hpy188III TCNNGA 1 cut(s) 208
Hpy99I CGWCG 7 cut(s) 62, 65, 161, 167, 296, 371, 374
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 2 cut(s) 303, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 159, 168
HpyF3I CTNAG 1 cut(s) 242
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 2 cut(s) 124, 289
HspAI GCGC 2 cut(s) 70, 107
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 81
LweI GCATC 1 cut(s) 397
MaeIII GTNAC 2 cut(s) 112, 389
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 3 cut(s) 19, 49, 73
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 1 cut(s) 423
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 8 cut(s) 17, 35, 82, 89, 182, 289, 343, 397
MreI CGCCGGCG 1 cut(s) 218
MroNI GCCGGC 1 cut(s) 218
MslI CAYNNNNRTG 1 cut(s) 119
MspI CCGG 4 cut(s) 34, 136, 186, 219
MspR9I CCNGG 2 cut(s) 98, 254
MvaI CCWGG 2 cut(s) 98, 254
MvnI CGCG 2 cut(s) 72, 107
MwoI GCNNNNNNNGC 3 cut(s) 126, 159, 168
NaeI GCCGGC 1 cut(s) 220
NdeII GATC 1 cut(s) 210
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 2 cut(s) 124, 289
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 2 cut(s) 124, 289
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 69, 219
PdiI GCCGGC 1 cut(s) 220
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 2 cut(s) 217, 356
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 96, 252
PspGI CCWGG 2 cut(s) 96, 252
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 119
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 2 cut(s) 216, 355
Sau3AI GATC 1 cut(s) 210
ScrFI CCNGG 2 cut(s) 98, 254
SduI GDGCHC 1 cut(s) 78
SetI ASST 4 cut(s) 78, 114, 195, 207
SfaNI GCATC 1 cut(s) 397
SgrAI CRCCGGYG 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 423
SsiI CCGC 3 cut(s) 169, 216, 354
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 96, 252
TaaI ACNGT 2 cut(s) 303, 335
TasI AATT 1 cut(s) 423
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 218, 357
TseFI GTSAC 1 cut(s) 112
Tsp45I GTSAC 1 cut(s) 112
Tth111I GACNNNGTC 1 cut(s) 161
XapI RAATTY 1 cut(s) 423
XceI RCATGY 2 cut(s) 124, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.