FvH4_7g20511

Leukotriene A-4 hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014067
Physical Location & Seq
Reverse (-)
16672536 .. 16675930
3395 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20511.t1

Sequence Viewer

Length: 1839 bp
ATGGCTCCGATCGACCCTCACTCGTTCACCGATTCGACTCACCCACTCGCCACCCACATTTCCCTCTCTCTCTTCTTCGACTTCTCCTCCTCCCTCATCCACGCCTCCGCCCTCCTCACCCTCCCCTCCCCTTACTCCGGCCCACTCTCCCTCGACGCGCGTGGCCTCACCGTCCTCTCCGTCCTCGACCCCCTCACCTCCTCCCCCCTCCCTTTCTCTCTCTCCTCCCCCGAACCAATCAAAGGCTGCCACCTCACCCTCACTCTCTCCAACCACTCCTCCGTCGTAATCGTCTACACCACCGCCCCAACCGCCTCCGCGCTCCAGTGGCTATCTCCGCCGCAGACGTTCAACAAGACGCTCCCGTTCGTCTACACGCAGTGCCAGGCCATCCACGCGCGGTCGGTTTTTCCTTGCCAGGACACCCCGGCGGCGCGTGTGTGCTACTCGGCGCGGCTGAACGTCCCGTGCCAGCTCTCCGCGGTCATGTCGGCGCGCCACGTCGACCGGAGGGACCCGATCGCCGGGGAGGGATCACTGCTGGCGTGCGGCGACGGCGCGTGGTGCGCGGAGGGGCGGACGGTGGAGGAGTTTGTGATGGAGCAGCCGATTCCGCCGTACCTGTTTGCGTTCGCCGTCGGGGAGCTGGGGTTCCGGGAGGTGGGACCGAGGACGAGGGTTTACTCGGAGGCGGTGCCGGCGGTGCTGGAAGCGGCGGCGAAGGAGTTTGCGAGCGCCGAGGACATGATCAGGCAAGGGGAGGCGTTGTTTGGGCCTTACGAGTGGGAGAGGTTTGACTTGCTGGTGCTGCCGCCGAGCTTTCCCTACGGCGGAATGGAGAATCCGAGGATGACGTTTCTGACGCCGACGGTGCTGAAAGGTGACTCGAGCGGCGCTCAGGTGGTGGCGCATGAGCTGGCTCATAGCTGGACTGGGAATTTGATCACTAACAAGACTAATGAGCACTTCTGGTTGAATGAGGGTTTCACCACGTATGCGGAGAGAAGAATTGTTGAGGCCGTGCAAGGTGAGGACAGAGCAGCATTGAATATTGGAATCGGTTGGAGGGGTTTGAATAAGGGAATGGAGAGGTTCAAGGATAACTTGGAGTTTACAAAGCTGAAAACCAACCAGGAAGGAGTGGACCCAGACGATGTGTATTCTGAAGTTCCATATGAAAAAGGTTTCCAGTTTCTGTGGCGCATTGAACGTCAGGTTGGAAGGCCTGCATTTGATGAATTTACGAAGAAGTATATTGCCACCTTCAAGTTCAAATCAATCGATACTGAAACATTTCTCGAATTTCTGAAAGCAAACCTACCTGGAATAGAGAAGGAGATTGACTTGACTCTGTGGACTGAGGGCACTGGTATCCCTCCTGATGCCTATGAACCAGTTTCCAGCATCTATACAAAGATTATATCAATGGCAAATGAATTTAAGCTTGGTAGGATGCCAAGGGAGGATGAAGTTGCCGATTGGGGGGGAATGGAGTGGGAGCTCTACTTGGAGAATCTGCCGAAATCTATTGAGGCCTCACAGATTTTAGACTTGAATGCTCGCTACAGTCTGTCTGAATCAAAAGATTATGAAGTGAAGGTGGCATTTCTTCTGCTGGCAATTGCTTCCAAGTGCAAAGATCATTATGCTGAGGTGGAAAAAACTTTGAAGGCAGTTGGAAGGAAGAAGTACCTTGTCCCACTATACACTGCTCTTGTAGAAGGCCCTGGTAAGGAAGAGGAGAAGATTTTGGCCAAGAGGGTGTTTGCAGAGGCTCGCGATACTTATCACCCTATAGCTCAAGGTGTGGTTGAGACGATCCTTAGCAAGCATATCTAG

Protein Analysis

613

Amino Acids

68.15

Weight (kDa)

5.27

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M1_N PF17900 118 - 162 2.6e-08 Peptidase M1 N-terminal domain
Peptidase_M1 PF01433 253 - 438 1.3e-41 Peptidase family M1 domain
Leuk-A4-hydro_C PF09127 495 - 608 6e-25 Leukotriene A4 hydrolase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014067)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13520
fragaria_vesca FvH4_7g20511
malus_domestica MD01G1113900.v1.1 MD01G1114100.v1.1 MD07G1182000.v1.1
prunus_persica Prupe.2G220700_v2.0.a1
pyrus_communis pycom01g14270 pycom01g14300
rosa_chinensis RchiOBHm_Chr1g0364401
rosa_laevigata RLG00000027483
rosa_multiflora Rmu_sc0001342.1_g000003
rosa_roxburghii Rroxscaffold_4G00292310
rosa_rugosa Rorug01G0316000
rosa_samantha Rh1BG286800 Rh1CG303400 Rh1DG318300
rosa_wichuraiana Rw1G028720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 694
AccBSI CCGCTC 1 cut(s) 891
AccI GTMKAC 3 cut(s) 294, 372, 504
AclWI GGATC 2 cut(s) 541, 1813
AcoI YGGCCR 1 cut(s) 1752
AcsI RAATTY 4 cut(s) 937, 1238, 1301, 1436
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 863
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 2 cut(s) 620, 1691
AfiI CCNNNNNNNGG 7 cut(s) 137, 242, 524, 661, 830, 1482, 1732
AjiI CACGTC 1 cut(s) 502
AjnI CCWGG 5 cut(s) 384, 417, 1131, 1321, 1726
AjuI GAANNNNNNNTTGG 4 cut(s) 1200, 1232, 1428, 1460
AloI GAACNNNNNNTCC 2 cut(s) 635, 667
AluBI AGCT 9 cut(s) 475, 646, 819, 916, 927, 1120, 1444, 1501, 1799
AluI AGCT 9 cut(s) 475, 646, 819, 916, 927, 1120, 1444, 1501, 1799
Alw21I GWGCWC 2 cut(s) 966, 1503
Alw26I GTCTC 1 cut(s) 1808
AlwI GGATC 2 cut(s) 541, 1813
AlwNI CAGNNNCTG 1 cut(s) 1195
Ama87I CYCGRG 1 cut(s) 886
AoxI GGCC 9 cut(s) 139, 163, 387, 773, 1017, 1223, 1533, 1723, 1752
ApeKI GCWGC 4 cut(s) 246, 604, 808, 1040
ApoI RAATTY 4 cut(s) 937, 1238, 1301, 1436
ArsI GACNNNNNNTTYG 2 cut(s) 922, 954
AscI GGCGCGCC 1 cut(s) 494
Asp700I GAANNNNTTC 1 cut(s) 1293
AspS9I GGNCC 6 cut(s) 140, 514, 665, 773, 1144, 1724
AsuC2I CCSGG 3 cut(s) 428, 526, 656
AvaI CYCGRG 1 cut(s) 886
AvaII GGWCC 3 cut(s) 514, 665, 1144
BaeGI GKGCMC 1 cut(s) 1367
BalI TGGCCA 1 cut(s) 1754
BanI GGYRCC 1 cut(s) 694
BanII GRGCYC 1 cut(s) 1503
Bbv12I GWGCWC 2 cut(s) 966, 1503
BbvCI CCTCAGC 1 cut(s) 1650
BbvI GCAGC 4 cut(s) 233, 616, 795, 1052
BccI CCATC 2 cut(s) 398, 592
BceAI ACGGC 5 cut(s) 571, 601, 620, 844, 1004
BciT130I CCWGG 5 cut(s) 386, 419, 1133, 1323, 1728
BciVI GTATCC 1 cut(s) 1382
BclI TGATCA 2 cut(s) 747, 942
BcnI CCSGG 3 cut(s) 428, 526, 656
BcoDI GTCTC 1 cut(s) 1808
BfaI CTAG 1 cut(s) 1837
BfmI CTRYAG 2 cut(s) 1564, 1794
BfoI RGCGCY 2 cut(s) 738, 897
BfuI GTATCC 1 cut(s) 1382
Bme1390I CCNGG 8 cut(s) 386, 419, 428, 526, 656, 1133, 1323, 1728
Bme18I GGWCC 3 cut(s) 514, 665, 1144
BmeT110I CYCGRG 1 cut(s) 886
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 6 cut(s) 140, 514, 665, 773, 1144, 1724
BmiI GGNNCC 7 cut(s) 6, 515, 516, 653, 666, 696, 1146
BmrFI CCNGG 8 cut(s) 386, 419, 428, 526, 656, 1133, 1323, 1728
BmrI ACTGGG 1 cut(s) 942
BmsI GCATC 3 cut(s) 1372, 1413, 1443
BmuI ACTGGG 1 cut(s) 942
BplI GAGNNNNNCTC 2 cut(s) 880, 912
BpmI CTGGAG 1 cut(s) 308
Bpu10I CCTNAGC 3 cut(s) 897, 1650, 1823
BpuEI CTTGAG 1 cut(s) 1785
BpuMI CCSGG 3 cut(s) 428, 526, 656
Bsa29I ATCGAT 1 cut(s) 1281
BsaAI YACGTR 1 cut(s) 993
BsaBI GATNNNNATC 1 cut(s) 1785
BsaHI GRCGYC 1 cut(s) 863
BsaJI CCNNGG 8 cut(s) 426, 480, 525, 668, 738, 845, 1457, 1726
BsaWI WCCGGW 1 cut(s) 507
BsaXI ACNNNNNCTCC 8 cut(s) 71, 101, 263, 293, 635, 665, 752, 782
Bsc4I CCNNNNNNNGG 7 cut(s) 137, 242, 524, 661, 830, 1482, 1732
Bse118I RCCGGY 1 cut(s) 697
Bse1I ACTGG 5 cut(s) 325, 937, 1189, 1372, 1394
Bse8I GATNNNNATC 1 cut(s) 1785
BseBI CCWGG 5 cut(s) 386, 419, 1133, 1323, 1728
BseCI ATCGAT 1 cut(s) 1281
BseDI CCNNGG 8 cut(s) 426, 480, 525, 668, 738, 845, 1457, 1726
BseGI GGATG 5 cut(s) 96, 390, 855, 1458, 1471
BseJI GATNNNNATC 1 cut(s) 1785
BseLI CCNNNNNNNGG 7 cut(s) 137, 242, 524, 661, 830, 1482, 1732
BseMII CTCAG 3 cut(s) 911, 1350, 1641
BseNI ACTGG 5 cut(s) 325, 937, 1189, 1372, 1394
BsePI GCGCGC 1 cut(s) 494
BseRI GAGGAG 8 cut(s) 76, 79, 104, 190, 214, 268, 602, 1754
BseSI GKGCMC 1 cut(s) 1367
BseXI GCAGC 4 cut(s) 233, 616, 795, 1052
BseYI CCCAGC 1 cut(s) 646
Bsh1285I CGRYCG 4 cut(s) 12, 404, 508, 522
BshFI GGCC 9 cut(s) 141, 165, 389, 775, 1019, 1225, 1535, 1725, 1754
BshNI GGYRCC 1 cut(s) 694
BshVI ATCGAT 1 cut(s) 1281
BsiEI CGRYCG 4 cut(s) 12, 404, 508, 522
BsiHKAI GWGCWC 2 cut(s) 966, 1503
BsiHKCI CYCGRG 1 cut(s) 886
BsiSI CCGG 6 cut(s) 138, 428, 508, 525, 655, 698
BslFI GGGAC 4 cut(s) 449, 527, 678, 1682
BslI CCNNNNNNNGG 7 cut(s) 137, 242, 524, 661, 830, 1482, 1732
BsmAI GTCTC 1 cut(s) 1808
BsmBI CGTCTC 1 cut(s) 1808
BsmFI GGGAC 4 cut(s) 449, 527, 678, 1682
BsmI GAATGC 1 cut(s) 1561
BsnI GGCC 9 cut(s) 141, 165, 389, 775, 1019, 1225, 1535, 1725, 1754
BsoBI CYCGRG 1 cut(s) 886
Bsp1286I GDGCHC 3 cut(s) 966, 1367, 1503
Bsp143I GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
Bsp68I TCGCGA 1 cut(s) 1779
BspANI GGCC 9 cut(s) 141, 165, 389, 775, 1019, 1225, 1535, 1725, 1754
BspCNI CTCAG 3 cut(s) 910, 1351, 1642
BspDI ATCGAT 1 cut(s) 1281
BspLI GGNNCC 7 cut(s) 6, 515, 516, 653, 666, 696, 1146
BspPI GGATC 2 cut(s) 541, 1813
BspT107I GGYRCC 1 cut(s) 694
BsrBI CCGCTC 1 cut(s) 891
BsrFI RCCGGY 1 cut(s) 697
BsrI ACTGG 5 cut(s) 325, 937, 1189, 1372, 1394
BssAI RCCGGY 1 cut(s) 697
BssECI CCNNGG 8 cut(s) 426, 480, 525, 668, 738, 845, 1457, 1726
BssHII GCGCGC 1 cut(s) 494
BssMI GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
BssNI GRCGYC 1 cut(s) 863
BssT1I CCWWGG 1 cut(s) 1457
Bst2UI CCWGG 5 cut(s) 386, 419, 1133, 1323, 1728
Bst4CI ACNGT 4 cut(s) 172, 583, 871, 1568
Bst6I CTCTTC 2 cut(s) 77, 1731
BstACI GRCGYC 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 993
BstDEI CTNAG 4 cut(s) 897, 1359, 1650, 1823
BstDSI CCRYGG 1 cut(s) 480
BstF5I GGATG 5 cut(s) 96, 390, 855, 1458, 1471
BstH2I RGCGCY 2 cut(s) 738, 897
BstKTI GATC 7 cut(s) 12, 522, 536, 750, 945, 1642, 1821
BstMAI GTCTC 1 cut(s) 1808
BstMBI GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
BstMCI CGRYCG 4 cut(s) 12, 404, 508, 522
BstNI CCWGG 5 cut(s) 386, 419, 1133, 1323, 1728
BstSCI CCNGG 8 cut(s) 384, 417, 426, 524, 654, 1131, 1321, 1726
BstSFI CTRYAG 2 cut(s) 1564, 1794
BstSLI GKGCMC 1 cut(s) 1367
BstV1I GCAGC 4 cut(s) 233, 616, 795, 1052
Bsu15I ATCGAT 1 cut(s) 1281
BsuI GTATCC 1 cut(s) 1382
BsuRI GGCC 9 cut(s) 141, 165, 389, 775, 1019, 1225, 1535, 1725, 1754
BsuTUI ATCGAT 1 cut(s) 1281
BtgI CCRYGG 1 cut(s) 480
BtrI CACGTC 1 cut(s) 502
BtsCI GGATG 5 cut(s) 96, 390, 855, 1458, 1471
BtsI GCAGTG 3 cut(s) 386, 536, 1707
BtsIMutI CAGTG 5 cut(s) 332, 386, 536, 1365, 1707
BtuMI TCGCGA 1 cut(s) 1779
CaiI CAGNNNCTG 1 cut(s) 1195
Cfr10I RCCGGY 1 cut(s) 697
Cfr13I GGNCC 6 cut(s) 140, 514, 665, 773, 1144, 1724
Cfr42I CCGCGG 1 cut(s) 483
ClaI ATCGAT 1 cut(s) 1281
CseI GACGC 3 cut(s) 164, 367, 871
Csp6I GTAC 2 cut(s) 619, 1690
CviAII CATG 3 cut(s) 487, 745, 911
CviQI GTAC 2 cut(s) 619, 1690
DdeI CTNAG 4 cut(s) 897, 1359, 1650, 1823
DpnI GATC 7 cut(s) 11, 521, 535, 749, 944, 1641, 1820
DpnII GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
DraIII CACNNNGTG 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 1752
Eam1104I CTCTTC 2 cut(s) 77, 1731
EarI CTCTTC 2 cut(s) 77, 1731
EciI GGCGGA 5 cut(s) 97, 327, 592, 603, 846
Ecl136II GAGCTC 1 cut(s) 1501
Eco130I CCWWGG 1 cut(s) 1457
Eco147I AGGCCT 2 cut(s) 1225, 1535
Eco24I GRGCYC 1 cut(s) 1503
Eco47I GGWCC 3 cut(s) 514, 665, 1144
Eco53kI GAGCTC 1 cut(s) 1501
Eco57I CTGAAG 1 cut(s) 1185
Eco88I CYCGRG 1 cut(s) 886
EcoICRI GAGCTC 1 cut(s) 1501
EcoO109I RGGNCCY 2 cut(s) 514, 1724
EcoRII CCWGG 5 cut(s) 384, 417, 1131, 1321, 1726
EcoT14I CCWWGG 1 cut(s) 1457
EcoT38I GRGCYC 1 cut(s) 1503
ErhI CCWWGG 1 cut(s) 1457
Esp3I CGTCTC 1 cut(s) 1808
FaeI CATG 3 cut(s) 490, 748, 914
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 4 cut(s) 449, 527, 678, 1682
FatI CATG 3 cut(s) 486, 744, 910
FauNDI CATATG 1 cut(s) 1174
FbaI TGATCA 2 cut(s) 747, 942
FblI GTMKAC 3 cut(s) 294, 372, 504
FokI GGATG 5 cut(s) 83, 377, 862, 1465, 1478
FriOI GRGCYC 1 cut(s) 1503
FspBI CTAG 1 cut(s) 1837
GsaI CCCAGC 1 cut(s) 650
GsuI CTGGAG 1 cut(s) 308
HaeII RGCGCY 2 cut(s) 738, 897
HaeIII GGCC 9 cut(s) 141, 165, 389, 775, 1019, 1225, 1535, 1725, 1754
HapII CCGG 6 cut(s) 138, 428, 508, 525, 655, 698
HgaI GACGC 3 cut(s) 164, 367, 871
Hin1I GRCGYC 1 cut(s) 863
Hin1II CATG 3 cut(s) 490, 748, 914
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 1442
HinfI GANTC 9 cut(s) 32, 37, 610, 841, 884, 1056, 1348, 1513, 1577
HpaII CCGG 6 cut(s) 138, 428, 508, 525, 655, 698
Hpy166II GTNNAC 8 cut(s) 27, 295, 373, 505, 682, 1113, 1144, 1356
Hpy188I TCNGA 7 cut(s) 9, 688, 846, 861, 1165, 1308, 1576
Hpy188III TCNNGA 3 cut(s) 1298, 1379, 1778
Hpy8I GTNNAC 8 cut(s) 27, 295, 373, 505, 682, 1113, 1144, 1356
Hpy99I CGWCG 6 cut(s) 158, 287, 506, 557, 641, 871
HpyAV CCTTC 9 cut(s) 715, 1130, 1215, 1273, 1327, 1591, 1663, 1674, 1715
HpyCH4III ACNGT 4 cut(s) 172, 583, 871, 1568
HpyCH4IV ACGT 6 cut(s) 347, 462, 501, 854, 992, 1210
HpyCH4V TGCA 4 cut(s) 1024, 1229, 1635, 1769
HpyF3I CTNAG 4 cut(s) 897, 1359, 1650, 1823
HpySE526I ACGT 6 cut(s) 347, 462, 501, 854, 992, 1210
Hsp92I GRCGYC 1 cut(s) 863
Hsp92II CATG 3 cut(s) 490, 748, 914
KflI GGGWCCC 1 cut(s) 514
KroI GCCGGC 1 cut(s) 697
KroNI GCCGGC 1 cut(s) 699
Ksp22I TGATCA 2 cut(s) 747, 942
KspI CCGCGG 1 cut(s) 483
Kzo9I GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
LmnI GCTCC 6 cut(s) 10, 327, 366, 601, 643, 1498
Lsp1109I GCAGC 4 cut(s) 233, 616, 795, 1052
LweI GCATC 3 cut(s) 1372, 1413, 1443
MaeI CTAG 1 cut(s) 1837
MaeII ACGT 6 cut(s) 347, 462, 501, 854, 992, 1210
MaeIII GTNAC 1 cut(s) 881
MalI GATC 7 cut(s) 11, 521, 535, 749, 944, 1641, 1820
MbiI CCGCTC 1 cut(s) 891
MboI GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
MboII GAAGA 8 cut(s) 64, 67, 1017, 1258, 1601, 1696, 1748, 1756
MfeI CAATTG 1 cut(s) 1620
MhlI GDGCHC 3 cut(s) 966, 1367, 1503
MlsI TGGCCA 1 cut(s) 1754
MluCI AATT 6 cut(s) 937, 1008, 1238, 1301, 1436, 1620
MluNI TGGCCA 1 cut(s) 1754
MlyI GAGTC 3 cut(s) 31, 878, 1342
MmeI TCCRAC 4 cut(s) 294, 1043, 1198, 1657
Mox20I TGGCCA 1 cut(s) 1754
MroNI GCCGGC 1 cut(s) 697
MroXI GAANNNNTTC 1 cut(s) 1293
MscI TGGCCA 1 cut(s) 1754
MseI TTAA 1 cut(s) 1440
Msp20I TGGCCA 1 cut(s) 1754
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 6 cut(s) 138, 428, 508, 525, 655, 698
MspR9I CCNGG 8 cut(s) 386, 419, 428, 526, 656, 1133, 1323, 1728
MunI CAATTG 1 cut(s) 1620
Mva1269I GAATGC 1 cut(s) 1561
MvaI CCWGG 5 cut(s) 386, 419, 1133, 1323, 1728
NaeI GCCGGC 1 cut(s) 699
NciI CCSGG 3 cut(s) 428, 526, 656
NdeI CATATG 1 cut(s) 1174
NdeII GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
NgoMIV GCCGGC 1 cut(s) 697
NlaIII CATG 3 cut(s) 490, 748, 914
NlaIV GGNNCC 7 cut(s) 6, 515, 516, 653, 666, 696, 1146
NmeAIII GCCGAG 3 cut(s) 428, 763, 840
NmuCI GTSAC 1 cut(s) 881
NruI TCGCGA 1 cut(s) 1779
PaeR7I CTCGAG 1 cut(s) 886
PalAI GGCGCGCC 1 cut(s) 494
PauI GCGCGC 1 cut(s) 494
PceI AGGCCT 2 cut(s) 1225, 1535
PctI GAATGC 1 cut(s) 1561
PdiI GCCGGC 1 cut(s) 699
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 6 cut(s) 32, 610, 841, 1056, 1513, 1577
PfoI TCCNGGA 1 cut(s) 654
Ple19I CGATCG 2 cut(s) 12, 522
PleI GAGTC 3 cut(s) 31, 878, 1342
PpsI GAGTC 3 cut(s) 31, 878, 1342
Ppu21I YACGTR 1 cut(s) 993
PpuMI RGGWCCY 1 cut(s) 514
Psp124BI GAGCTC 1 cut(s) 1503
Psp5II RGGWCCY 1 cut(s) 514
Psp6I CCWGG 5 cut(s) 384, 417, 1131, 1321, 1726
PspFI CCCAGC 1 cut(s) 646
PspGI CCWGG 5 cut(s) 384, 417, 1131, 1321, 1726
PspN4I GGNNCC 7 cut(s) 6, 515, 516, 653, 666, 696, 1146
PspPI GGNCC 6 cut(s) 140, 514, 665, 773, 1144, 1724
PspPPI RGGWCCY 1 cut(s) 514
PspXI VCTCGAGB 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 1195
PteI GCGCGC 1 cut(s) 494
PvuI CGATCG 2 cut(s) 12, 522
RruI TCGCGA 1 cut(s) 1779
RsaI GTAC 2 cut(s) 620, 1691
RsaNI GTAC 2 cut(s) 619, 1690
SacI GAGCTC 1 cut(s) 1503
SacII CCGCGG 1 cut(s) 483
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 1 cut(s) 1440
Sau3AI GATC 7 cut(s) 9, 519, 533, 747, 942, 1639, 1818
Sau96I GGNCC 6 cut(s) 140, 514, 665, 773, 1144, 1724
SchI GAGTC 3 cut(s) 31, 878, 1342
ScrFI CCNGG 8 cut(s) 386, 419, 428, 526, 656, 1133, 1323, 1728
SduI GDGCHC 3 cut(s) 966, 1367, 1503
SfaNI GCATC 3 cut(s) 1372, 1413, 1443
SfcI CTRYAG 2 cut(s) 1564, 1794
Sfr274I CTCGAG 1 cut(s) 886
Sfr303I CCGCGG 1 cut(s) 483
SgrBI CCGCGG 1 cut(s) 483
SgsI GGCGCGCC 1 cut(s) 494
SinI GGWCC 3 cut(s) 514, 665, 1144
SlaI CTCGAG 1 cut(s) 886
SmlI CTYRAG 2 cut(s) 886, 1800
SmoI CTYRAG 2 cut(s) 886, 1800
Sse9I AATT 6 cut(s) 937, 1008, 1238, 1301, 1436, 1620
SseBI AGGCCT 2 cut(s) 1225, 1535
SspI AATATT 1 cut(s) 1051
SspMI CTAG 1 cut(s) 1837
SstI GAGCTC 1 cut(s) 1503
StuI AGGCCT 2 cut(s) 1225, 1535
StyD4I CCNGG 8 cut(s) 384, 417, 426, 524, 654, 1131, 1321, 1726
StyI CCWWGG 1 cut(s) 1457
TaaI ACNGT 4 cut(s) 172, 583, 871, 1568
TaiI ACGT 6 cut(s) 350, 465, 504, 857, 995, 1213
TaqI TCGA 9 cut(s) 12, 35, 78, 153, 186, 504, 887, 1281, 1299
TaqII GACCGA 1 cut(s) 682
TasI AATT 6 cut(s) 937, 1008, 1238, 1301, 1436, 1620
TauI GCSGC 8 cut(s) 343, 434, 457, 552, 716, 719, 814, 894
TfiI GAWTC 6 cut(s) 32, 610, 841, 1056, 1513, 1577
Tru1I TTAA 1 cut(s) 1440
Tru9I TTAA 1 cut(s) 1440
TscAI CASTG 5 cut(s) 332, 386, 543, 1372, 1714
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 4 cut(s) 246, 604, 808, 1040
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 6 cut(s) 1191, 1251, 1404, 1449, 1482, 1605
TspGWI ACGGA 2 cut(s) 169, 271
TspRI CASTG 5 cut(s) 332, 386, 543, 1372, 1714
VpaK11BI GGWCC 3 cut(s) 514, 665, 1144
XapI RAATTY 4 cut(s) 937, 1238, 1301, 1436
XhoI CTCGAG 1 cut(s) 886
XmiI GTMKAC 3 cut(s) 294, 372, 504
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 1 cut(s) 1837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.