Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0014067

Physical Location & Seq

Genomic Coordinates Forward (+)

12656123 .. 12659318

Gene Structure 3196 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00292310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGGCTCCGATCGACCCTCACTCGTTCACCGATTCGACTCACCCACTCGCCACCCACATTTCCCTCTCTCTCTTCTTCGACTTCTCCTCCTCCACCATCCACGCCTCCGCCCTCCTCACCCTCCCGGCCCCTCACTCCGGCCCACTCTCCCTCGACTCGCGTGGCCTCACCGTCCACTCCGTCCTCGACCCCCAATCCTCTGCTCCCCTTCCTTTCTCCCTCTCCTCCCCCGACCCAATCAAAGGCTGCCACCTCACCGTCACTCTCGCCAACCAATCCTCGTGGCTCTCGCCGCCGCAGACCTTCAACAAAACGCTGCCGTTTGTGTACACTCAGTGCCAGGCCATCCACGCGCGGTCTGTGTTCCCGTGCCAGGACACCCCGGCGGCGCGTGTGTGCTACTCGGCAAGGTTGAACGTCCCGCGCCAGCTCTCCGCCGTCATGTCGGCTCGCCACGTCGACCGCAGGGACCCGATCGCCGGGGAGGGATCGGCGCTGGGGTGCGGCGACGGAGCGTGGTGCGCGGAGGGGAGGACGGTGGAGGAGTTCGTGATGGAGCAGCCGATTCCGCCGTACCTGTTCGCGTTCGCCGTCGGGGAGCTAGGGTTCCGGGAGGTGGGACCGAGGACCAGGGTTTACTCGGAGGCCGTGCCGGCGGTGCTGGAATTGGCGGCGAAGGAGTTTGCGAGCGCCGAGGACATGATCAGGCAAGGGGAGGCCTTGTTTGGGCCTTACGAGTGGGAGAGGTTTGATTTGCTGGTGCTGCCGCCGAGCTTTCCTTACGGCGGAATGGAGAATCCGAGGATGACGTTTCTGACGCCGACGGTGCTGAAAGGTGACTCGAGCGGCGCACAGGTGGTGGCGCATGAGCTGGCTCATAGTTGGACCGGGAATTTGATCACTAACAAGACTAATGAGCACTTCTGGTTGAATGAGGGTTTCACAACATATGCAGAGAGAAGAATTGTTGAGGCTGTGCAAGGTGAGGACAGAGCTGCACTGAATATTGGAATTGGTTGGAAGGGTTTAAATAAGGAAATGGAGAGGTTCAAGGATAACATGGAGTTCACAAAGCTGAAAACCAATCAGGAAGGAGTGGACCCAGATGATGTGTATTCTGAAGTTCCATATGAAAAGGGTTTCCAGTTTCTGTGGCGCATTGAACGTCAGGTTGGAAGGCCTGCATTTGATGAATTTACAAAAACGTATATTGCCACCTTCAAGTTCAAATCAATTGATACCGAAACATTTCTCGAATTTCTGAAAGCAAACCTACCTGGAATAGAGAAAGAGATTGACTTGATTCTGTGGACTGAGGGCACTGGTATCCCTCCTGATGCCTATGAACCAGTTTCCAGCATCTATACAAAAATTATATCGATGGCAAATGAATTTAAGCTTGGTAGGATGCCGAGGGAGGATGAAGTTGCTGACTGGGGAGGAATGGAGTGGGAGCTCTACTTGGAGAACCTGCCCAAATCTATTGAAGCTTCACAGATTTTAGACTTGAATGCTCGCTACAGTCTGTCTGAATCGAAAGATTATGAAGTAAAGGTGGCATTTCTTCTGCTGGCAATTGGTTCCAAGTGCAAAGATCACTATGCTGAGGTAGAAAAAACTTTGAAGGCAGTCGGGAGGAAGAAGTACCTTGTCCCACTATACACTGCTCTTGTAGAAGGCACTGGTAAGGAAGAGGAGAAGATTTTGGCCAAGAGGGTGTTTGCGGAGGCTCGCGAAACTTATCACCCTATAGCTCAAGGTGTTGTTGAGACAATCCTTAGCAAGCACATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0004301 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006518 GO:0006629 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0008270 GO:0009056 GO:0009987 GO:0016787 GO:0016801 GO:0016803 GO:0019538 GO:0033218 GO:0034641 GO:0042277 GO:0043167 GO:0043169 GO:0043170 GO:0043171 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0070006 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00590 ko01100 map00590 map01100

Pfam Domains

Protein Families

Leuk-A4-hydro_C Peptidase_M1

Protein Analysis

597

Amino Acids

66.65

Weight (kDa)

5.27

Isoelectric Point (pI)

37.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M1_N	PF17900	104 - 147	1.9e-08	Peptidase M1 N-terminal domain
Peptidase_M1	PF01433	238 - 424	2e-42	Peptidase family M1 domain
Leuk-A4-hydro_C	PF09127	480 - 593	7.2e-25	Leukotriene A4 hydrolase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13520
fragaria_vesca	FvH4_7g20511
malus_domestica	MD01G1113900.v1.1 MD01G1114100.v1.1 MD07G1182000.v1.1
prunus_persica	Prupe.2G220700_v2.0.a1
pyrus_communis	pycom01g14270 pycom01g14300
rosa_chinensis	RchiOBHm_Chr1g0364401
rosa_laevigata	RLG00000027483
rosa_multiflora	Rmu_sc0001342.1_g000003
rosa_roxburghii	Rroxscaffold_4G00292310
rosa_rugosa	Rorug01G0316000
rosa_samantha	Rh1BG286800 Rh1CG303400 Rh1DG318300
rosa_wichuraiana	Rw1G028720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1479
AccBSI	CCGCTC	1 cut(s)	846
AccI	GTMKAC	1 cut(s)	459
AccII	CGCG	8 cut(s)	160, 353, 355, 391, 424, 524, 584, 1734
AclWI	GGATC	1 cut(s)	496
AcoI	YGGCCR	1 cut(s)	1707
AcsI	RAATTY	4 cut(s)	892, 1193, 1256, 1391
AcuI	CTGAAG	1 cut(s)	1140
AcyI	GRCGYC	1 cut(s)	818
AdeI	CACNNNGTG	1 cut(s)	336
AfaI	GTAC	3 cut(s)	329, 575, 1646
AfiI	CCNNNNNNNGG	7 cut(s)	137, 242, 373, 479, 616, 726, 785
AjiI	CACGTC	1 cut(s)	457
AjnI	CCWGG	4 cut(s)	339, 372, 629, 1276
AjuI	GAANNNNNNNTTGG	4 cut(s)	1155, 1187, 1383, 1415
AloI	GAACNNNNNNTCC	2 cut(s)	590, 622
Alw21I	GWGCWC	2 cut(s)	921, 1458
Alw26I	GTCTC	1 cut(s)	1763
AlwI	GGATC	1 cut(s)	496
AlwNI	CAGNNNCTG	1 cut(s)	1150
Ama87I	CYCGRG	1 cut(s)	841
AoxI	GGCC	9 cut(s)	126, 139, 163, 342, 645, 717, 728, 1178, 1707
ApeKI	GCWGC	5 cut(s)	246, 316, 559, 763, 995
ApoI	RAATTY	4 cut(s)	892, 1193, 1256, 1391
ArsI	GACNNNNNNTTYG	2 cut(s)	877, 909
Asp700I	GAANNNNTTC	1 cut(s)	1248
AspLEI	GCGC	9 cut(s)	355, 391, 426, 496, 524, 692, 851, 865, 1158
AspS9I	GGNCC	8 cut(s)	127, 140, 469, 620, 627, 728, 885, 1099
AsuC2I	CCSGG	5 cut(s)	125, 383, 481, 611, 889
AsuHPI	GGTGA	8 cut(s)	19, 32, 109, 160, 247, 848, 995, 1736
AvaI	CYCGRG	1 cut(s)	841
AvaII	GGWCC	5 cut(s)	469, 620, 627, 885, 1099
BaeGI	GKGCMC	1 cut(s)	1322
BalI	TGGCCA	1 cut(s)	1709
BanII	GRGCYC	1 cut(s)	1458
BauI	CACGAG	1 cut(s)	280
Bbv12I	GWGCWC	2 cut(s)	921, 1458
BbvCI	CCTCAGC	1 cut(s)	1605
BbvI	GCAGC	5 cut(s)	233, 303, 571, 750, 982
BccI	CCATC	4 cut(s)	104, 353, 547, 1375
BceAI	ACGGC	6 cut(s)	304, 422, 556, 575, 632, 799
BciT130I	CCWGG	4 cut(s)	341, 374, 631, 1278
BciVI	GTATCC	1 cut(s)	1337
BclI	TGATCA	2 cut(s)	702, 897
BcnI	CCSGG	5 cut(s)	125, 383, 481, 611, 889
BcoDI	GTCTC	1 cut(s)	1763
BfaI	CTAG	2 cut(s)	602, 1792
BfmI	CTRYAG	2 cut(s)	1519, 1749
BfoI	RGCGCY	2 cut(s)	497, 693
BfuAI	ACCTGC	1 cut(s)	1479
BfuI	GTATCC	1 cut(s)	1337
BglI	GCCNNNNNGGC	1 cut(s)	653
Bme1390I	CCNGG	9 cut(s)	125, 341, 374, 383, 481, 611, 631, 889, 1278
Bme18I	GGWCC	5 cut(s)	469, 620, 627, 885, 1099
BmeT110I	CYCGRG	1 cut(s)	841
BmgBI	CACGTC	1 cut(s)	457
BmgT120I	GGNCC	8 cut(s)	127, 140, 469, 620, 627, 728, 885, 1099
BmiI	GGNNCC	8 cut(s)	6, 129, 470, 471, 608, 621, 1101, 1582
BmrFI	CCNGG	9 cut(s)	125, 341, 374, 383, 481, 611, 631, 889, 1278
BmrI	ACTGGG	1 cut(s)	1444
BmsI	GCATC	3 cut(s)	1327, 1368, 1398
BmuI	ACTGGG	1 cut(s)	1444
Bpu10I	CCTNAGC	2 cut(s)	1605, 1778
BpuEI	CTTGAG	1 cut(s)	1740
BpuMI	CCSGG	5 cut(s)	125, 383, 481, 611, 889
Bsa29I	ATCGAT	1 cut(s)	1379
BsaHI	GRCGYC	1 cut(s)	818
BsaJI	CCNNGG	7 cut(s)	381, 480, 623, 630, 693, 800, 1412
BsaXI	ACNNNNNCTCC	6 cut(s)	71, 101, 590, 620, 707, 737
Bsc4I	CCNNNNNNNGG	7 cut(s)	137, 242, 373, 479, 616, 726, 785
Bse118I	RCCGGY	1 cut(s)	652
Bse1I	ACTGG	5 cut(s)	1144, 1327, 1349, 1439, 1687
BseBI	CCWGG	4 cut(s)	341, 374, 631, 1278
BseCI	ATCGAT	1 cut(s)	1379
BseDI	CCNNGG	7 cut(s)	381, 480, 623, 630, 693, 800, 1412
BseGI	GGATG	5 cut(s)	96, 345, 810, 1413, 1426
BseLI	CCNNNNNNNGG	7 cut(s)	137, 242, 373, 479, 616, 726, 785
BseMII	CTCAG	3 cut(s)	347, 1305, 1596
BseNI	ACTGG	5 cut(s)	1144, 1327, 1349, 1439, 1687
BseRI	GAGGAG	6 cut(s)	76, 79, 104, 214, 557, 1709
BseSI	GKGCMC	1 cut(s)	1322
BseXI	GCAGC	5 cut(s)	233, 303, 571, 750, 982
BseYI	CCCAGC	1 cut(s)	496
BsgI	GTGCAG	1 cut(s)	981
Bsh1236I	CGCG	8 cut(s)	160, 353, 355, 391, 424, 524, 584, 1734
Bsh1285I	CGRYCG	3 cut(s)	12, 463, 477
BshFI	GGCC	9 cut(s)	128, 141, 165, 344, 647, 719, 730, 1180, 1709
BshVI	ATCGAT	1 cut(s)	1379
BsiEI	CGRYCG	3 cut(s)	12, 463, 477
BsiHKAI	GWGCWC	2 cut(s)	921, 1458
BsiHKCI	CYCGRG	1 cut(s)	841
BsiSI	CCGG	7 cut(s)	125, 138, 383, 480, 610, 653, 888
BslFI	GGGAC	4 cut(s)	404, 482, 633, 1637
BslI	CCNNNNNNNGG	7 cut(s)	137, 242, 373, 479, 616, 726, 785
BsmAI	GTCTC	1 cut(s)	1763
BsmFI	GGGAC	4 cut(s)	404, 482, 633, 1637
BsmI	GAATGC	1 cut(s)	1516
BsnI	GGCC	9 cut(s)	128, 141, 165, 344, 647, 719, 730, 1180, 1709
BsoBI	CYCGRG	1 cut(s)	841
Bsp1286I	GDGCHC	3 cut(s)	921, 1322, 1458
Bsp1407I	TGTACA	1 cut(s)	327
Bsp143I	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
Bsp68I	TCGCGA	1 cut(s)	1734
BspANI	GGCC	9 cut(s)	128, 141, 165, 344, 647, 719, 730, 1180, 1709
BspCNI	CTCAG	3 cut(s)	346, 1306, 1597
BspDI	ATCGAT	1 cut(s)	1379
BspFNI	CGCG	8 cut(s)	160, 353, 355, 391, 424, 524, 584, 1734
BspLI	GGNNCC	8 cut(s)	6, 129, 470, 471, 608, 621, 1101, 1582
BspMI	ACCTGC	1 cut(s)	1479
BspPI	GGATC	1 cut(s)	496
BsrBI	CCGCTC	1 cut(s)	846
BsrFI	RCCGGY	1 cut(s)	652
BsrGI	TGTACA	1 cut(s)	327
BsrI	ACTGG	5 cut(s)	1144, 1327, 1349, 1439, 1687
BssAI	RCCGGY	1 cut(s)	652
BssECI	CCNNGG	7 cut(s)	381, 480, 623, 630, 693, 800, 1412
BssMI	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
BssNI	GRCGYC	1 cut(s)	818
BssSI	CACGAG	1 cut(s)	280
Bst2BI	CACGAG	1 cut(s)	280
Bst2UI	CCWGG	4 cut(s)	341, 374, 631, 1278
Bst4CI	ACNGT	5 cut(s)	172, 259, 538, 826, 1523
Bst6I	CTCTTC	2 cut(s)	77, 1686
BstACI	GRCGYC	1 cut(s)	818
BstAUI	TGTACA	1 cut(s)	327
BstDEI	CTNAG	4 cut(s)	333, 1314, 1605, 1778
BstF5I	GGATG	5 cut(s)	96, 345, 810, 1413, 1426
BstFNI	CGCG	8 cut(s)	160, 353, 355, 391, 424, 524, 584, 1734
BstH2I	RGCGCY	2 cut(s)	497, 693
BstHHI	GCGC	9 cut(s)	355, 391, 426, 496, 524, 692, 851, 865, 1158
BstKTI	GATC	6 cut(s)	12, 477, 491, 705, 900, 1597
BstMAI	GTCTC	1 cut(s)	1763
BstMBI	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
BstMCI	CGRYCG	3 cut(s)	12, 463, 477
BstMWI	GCNNNNNNNGC	8 cut(s)	292, 350, 521, 568, 653, 658, 763, 826
BstNI	CCWGG	4 cut(s)	341, 374, 631, 1278
BstSCI	CCNGG	9 cut(s)	123, 339, 372, 381, 479, 609, 629, 887, 1276
BstSFI	CTRYAG	2 cut(s)	1519, 1749
BstSLI	GKGCMC	1 cut(s)	1322
BstUI	CGCG	8 cut(s)	160, 353, 355, 391, 424, 524, 584, 1734
BstV1I	GCAGC	5 cut(s)	233, 303, 571, 750, 982
Bsu15I	ATCGAT	1 cut(s)	1379
BsuI	GTATCC	1 cut(s)	1337
BsuRI	GGCC	9 cut(s)	128, 141, 165, 344, 647, 719, 730, 1180, 1709
BsuTUI	ATCGAT	1 cut(s)	1379
BtrI	CACGTC	1 cut(s)	457
BtsCI	GGATG	5 cut(s)	96, 345, 810, 1413, 1426
BtsI	GCAGTG	1 cut(s)	1662
BtsIMutI	CAGTG	5 cut(s)	341, 998, 1320, 1662, 1680
BtuMI	TCGCGA	1 cut(s)	1734
BveI	ACCTGC	1 cut(s)	1479
CaiI	CAGNNNCTG	1 cut(s)	1150
CfoI	GCGC	9 cut(s)	355, 391, 426, 496, 524, 692, 851, 865, 1158
Cfr10I	RCCGGY	1 cut(s)	652
Cfr13I	GGNCC	8 cut(s)	127, 140, 469, 620, 627, 728, 885, 1099
ClaI	ATCGAT	1 cut(s)	1379
CseI	GACGC	1 cut(s)	826
Csp6I	GTAC	3 cut(s)	328, 574, 1645
CspCI	CAANNNNNGTGG	2 cut(s)	263, 298
CviAII	CATG	4 cut(s)	442, 700, 866, 1060
CviQI	GTAC	3 cut(s)	328, 574, 1645
DdeI	CTNAG	4 cut(s)	333, 1314, 1605, 1778
DpnI	GATC	6 cut(s)	11, 476, 490, 704, 899, 1596
DpnII	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
DraI	TTTAAA	1 cut(s)	1029
DraIII	CACNNNGTG	1 cut(s)	336
EaeI	YGGCCR	1 cut(s)	1707
Eam1104I	CTCTTC	2 cut(s)	77, 1686
EarI	CTCTTC	2 cut(s)	77, 1686
EciI	GGCGGA	4 cut(s)	97, 424, 558, 801
Ecl136II	GAGCTC	1 cut(s)	1456
Eco147I	AGGCCT	2 cut(s)	719, 1180
Eco24I	GRGCYC	1 cut(s)	1458
Eco47I	GGWCC	5 cut(s)	469, 620, 627, 885, 1099
Eco53kI	GAGCTC	1 cut(s)	1456
Eco57I	CTGAAG	1 cut(s)	1140
Eco88I	CYCGRG	1 cut(s)	841
EcoICRI	GAGCTC	1 cut(s)	1456
EcoO109I	RGGNCCY	1 cut(s)	469
EcoRII	CCWGG	4 cut(s)	339, 372, 629, 1276
EcoT38I	GRGCYC	1 cut(s)	1458
FaeI	CATG	4 cut(s)	445, 703, 869, 1063
FaqI	GGGAC	4 cut(s)	404, 482, 633, 1637
FatI	CATG	4 cut(s)	441, 699, 865, 1059
FauI	CCCGC	1 cut(s)	429
FauNDI	CATATG	2 cut(s)	949, 1129
FbaI	TGATCA	2 cut(s)	702, 897
FblI	GTMKAC	1 cut(s)	459
FokI	GGATG	5 cut(s)	83, 332, 817, 1420, 1433
FriOI	GRGCYC	1 cut(s)	1458
FspBI	CTAG	2 cut(s)	602, 1792
GlaI	GCGC	9 cut(s)	354, 390, 425, 495, 523, 691, 850, 864, 1157
GsaI	CCCAGC	1 cut(s)	500
HaeII	RGCGCY	2 cut(s)	497, 693
HaeIII	GGCC	9 cut(s)	128, 141, 165, 344, 647, 719, 730, 1180, 1709
HapII	CCGG	7 cut(s)	125, 138, 383, 480, 610, 653, 888
HgaI	GACGC	1 cut(s)	826
HhaI	GCGC	9 cut(s)	355, 391, 426, 496, 524, 692, 851, 865, 1158
Hin1I	GRCGYC	1 cut(s)	818
Hin1II	CATG	4 cut(s)	445, 703, 869, 1063
Hin6I	GCGC	9 cut(s)	353, 389, 424, 494, 522, 690, 849, 863, 1156
HinP1I	GCGC	9 cut(s)	353, 389, 424, 494, 522, 690, 849, 863, 1156
HincII	GTYRAC	1 cut(s)	460
HindII	GTYRAC	1 cut(s)	460
HindIII	AAGCTT	2 cut(s)	1397, 1488
HinfI	GANTC	8 cut(s)	32, 37, 155, 565, 796, 839, 1303, 1532
HpaII	CCGG	7 cut(s)	125, 138, 383, 480, 610, 653, 888
HphI	GGTGA	8 cut(s)	19, 32, 109, 160, 247, 848, 995, 1736
Hpy166II	GTNNAC	9 cut(s)	27, 175, 328, 330, 460, 637, 1068, 1099, 1311
Hpy188I	TCNGA	7 cut(s)	9, 643, 801, 816, 1120, 1263, 1531
Hpy188III	TCNNGA	6 cut(s)	550, 1088, 1253, 1334, 1633, 1733
Hpy8I	GTNNAC	9 cut(s)	27, 175, 328, 330, 460, 637, 1068, 1099, 1311
Hpy99I	CGWCG	4 cut(s)	461, 512, 596, 826
HpyAV	CCTTC	9 cut(s)	218, 313, 670, 1015, 1085, 1170, 1228, 1618, 1670
HpyCH4III	ACNGT	5 cut(s)	172, 259, 538, 826, 1523
HpyCH4IV	ACGT	5 cut(s)	417, 456, 809, 1165, 1205
HpyCH4V	TGCA	5 cut(s)	953, 979, 998, 1184, 1590
HpyF10VI	GCNNNNNNNGC	8 cut(s)	292, 350, 521, 568, 653, 658, 763, 826
HpyF3I	CTNAG	4 cut(s)	333, 1314, 1605, 1778
HpySE526I	ACGT	5 cut(s)	417, 456, 809, 1165, 1205
Hsp92I	GRCGYC	1 cut(s)	818
Hsp92II	CATG	4 cut(s)	445, 703, 869, 1063
HspAI	GCGC	9 cut(s)	353, 389, 424, 494, 522, 690, 849, 863, 1156
KflI	GGGWCCC	1 cut(s)	469
KroI	GCCGGC	1 cut(s)	652
KroNI	GCCGGC	1 cut(s)	654
Ksp22I	TGATCA	2 cut(s)	702, 897
Kzo9I	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
LmnI	GCTCC	6 cut(s)	10, 208, 512, 556, 598, 1453
Lsp1109I	GCAGC	5 cut(s)	233, 303, 571, 750, 982
LweI	GCATC	3 cut(s)	1327, 1368, 1398
MaeI	CTAG	2 cut(s)	602, 1792
MaeII	ACGT	5 cut(s)	417, 456, 809, 1165, 1205
MaeIII	GTNAC	2 cut(s)	259, 836
MalI	GATC	6 cut(s)	11, 476, 490, 704, 899, 1596
MbiI	CCGCTC	1 cut(s)	846
MboI	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
MboII	GAAGA	7 cut(s)	64, 67, 972, 1556, 1651, 1703, 1711
MfeI	CAATTG	2 cut(s)	1233, 1575
MhlI	GDGCHC	3 cut(s)	921, 1322, 1458
MlsI	TGGCCA	1 cut(s)	1709
MluNI	TGGCCA	1 cut(s)	1709
MlyI	GAGTC	3 cut(s)	31, 149, 833
MmeI	TCCRAC	3 cut(s)	863, 998, 1153
Mox20I	TGGCCA	1 cut(s)	1709
MroNI	GCCGGC	1 cut(s)	652
MroXI	GAANNNNTTC	1 cut(s)	1248
MscI	TGGCCA	1 cut(s)	1709
MseI	TTAA	2 cut(s)	1028, 1395
Msp20I	TGGCCA	1 cut(s)	1709
MspI	CCGG	7 cut(s)	125, 138, 383, 480, 610, 653, 888
MspR9I	CCNGG	9 cut(s)	125, 341, 374, 383, 481, 611, 631, 889, 1278
MunI	CAATTG	2 cut(s)	1233, 1575
Mva1269I	GAATGC	1 cut(s)	1516
MvaI	CCWGG	4 cut(s)	341, 374, 631, 1278
MvnI	CGCG	8 cut(s)	160, 353, 355, 391, 424, 524, 584, 1734
MwoI	GCNNNNNNNGC	8 cut(s)	292, 350, 521, 568, 653, 658, 763, 826
NaeI	GCCGGC	1 cut(s)	654
NciI	CCSGG	5 cut(s)	125, 383, 481, 611, 889
NdeI	CATATG	2 cut(s)	949, 1129
NdeII	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
NgoMIV	GCCGGC	1 cut(s)	652
NlaIII	CATG	4 cut(s)	445, 703, 869, 1063
NlaIV	GGNNCC	8 cut(s)	6, 129, 470, 471, 608, 621, 1101, 1582
NmeAIII	GCCGAG	4 cut(s)	383, 718, 795, 1437
NmuCI	GTSAC	2 cut(s)	259, 836
NruI	TCGCGA	1 cut(s)	1734
PaeR7I	CTCGAG	1 cut(s)	841
PceI	AGGCCT	2 cut(s)	719, 1180
PcsI	WCGNNNNNNNCGW	1 cut(s)	588
PctI	GAATGC	1 cut(s)	1516
PdiI	GCCGGC	1 cut(s)	654
PdmI	GAANNNNTTC	1 cut(s)	1248
PfeI	GAWTC	5 cut(s)	32, 565, 796, 1303, 1532
PfoI	TCCNGGA	1 cut(s)	609
Ple19I	CGATCG	2 cut(s)	12, 477
PleI	GAGTC	3 cut(s)	31, 149, 833
PpsI	GAGTC	3 cut(s)	31, 149, 833
PpuMI	RGGWCCY	1 cut(s)	469
Psp124BI	GAGCTC	1 cut(s)	1458
Psp5II	RGGWCCY	1 cut(s)	469
Psp6I	CCWGG	4 cut(s)	339, 372, 629, 1276
PspFI	CCCAGC	1 cut(s)	496
PspGI	CCWGG	4 cut(s)	339, 372, 629, 1276
PspN4I	GGNNCC	8 cut(s)	6, 129, 470, 471, 608, 621, 1101, 1582
PspPI	GGNCC	8 cut(s)	127, 140, 469, 620, 627, 728, 885, 1099
PspPPI	RGGWCCY	1 cut(s)	469
PspXI	VCTCGAGB	1 cut(s)	841
PstNI	CAGNNNCTG	1 cut(s)	1150
PvuI	CGATCG	2 cut(s)	12, 477
RruI	TCGCGA	1 cut(s)	1734
RsaI	GTAC	3 cut(s)	329, 575, 1646
RsaNI	GTAC	3 cut(s)	328, 574, 1645
SacI	GAGCTC	1 cut(s)	1458
SalI	GTCGAC	1 cut(s)	458
SaqAI	TTAA	2 cut(s)	1028, 1395
Sau3AI	GATC	6 cut(s)	9, 474, 488, 702, 897, 1594
Sau96I	GGNCC	8 cut(s)	127, 140, 469, 620, 627, 728, 885, 1099
SchI	GAGTC	3 cut(s)	31, 149, 833
ScrFI	CCNGG	9 cut(s)	125, 341, 374, 383, 481, 611, 631, 889, 1278
SduI	GDGCHC	3 cut(s)	921, 1322, 1458
SfaNI	GCATC	3 cut(s)	1327, 1368, 1398
SfcI	CTRYAG	2 cut(s)	1519, 1749
Sfr274I	CTCGAG	1 cut(s)	841
SinI	GGWCC	5 cut(s)	469, 620, 627, 885, 1099
SlaI	CTCGAG	1 cut(s)	841
SmlI	CTYRAG	2 cut(s)	841, 1755
SmoI	CTYRAG	2 cut(s)	841, 1755
SseBI	AGGCCT	2 cut(s)	719, 1180
SspI	AATATT	1 cut(s)	1006
SspMI	CTAG	2 cut(s)	602, 1792
SstI	GAGCTC	1 cut(s)	1458
StuI	AGGCCT	2 cut(s)	719, 1180
StyD4I	CCNGG	9 cut(s)	123, 339, 372, 381, 479, 609, 629, 887, 1276
TaaI	ACNGT	5 cut(s)	172, 259, 538, 826, 1523
TaiI	ACGT	5 cut(s)	420, 459, 812, 1168, 1208
TaqII	GACCGA	1 cut(s)	637
TatI	WGTACW	1 cut(s)	327
TauI	GCSGC	7 cut(s)	295, 298, 389, 507, 674, 769, 849
TfiI	GAWTC	5 cut(s)	32, 565, 796, 1303, 1532
Tru1I	TTAA	2 cut(s)	1028, 1395
Tru9I	TTAA	2 cut(s)	1028, 1395
TscAI	CASTG	5 cut(s)	341, 1005, 1327, 1669, 1687
TseFI	GTSAC	2 cut(s)	259, 836
TseI	GCWGC	5 cut(s)	246, 316, 559, 763, 995
Tsp45I	GTSAC	2 cut(s)	259, 836
TspDTI	ATGAA	6 cut(s)	1146, 1206, 1359, 1404, 1437, 1560
TspGWI	ACGGA	2 cut(s)	169, 525
TspRI	CASTG	5 cut(s)	341, 1005, 1327, 1669, 1687
VpaK11BI	GGWCC	5 cut(s)	469, 620, 627, 885, 1099
XapI	RAATTY	4 cut(s)	892, 1193, 1256, 1391
XhoI	CTCGAG	1 cut(s)	841
XmiI	GTMKAC	1 cut(s)	459
XmnI	GAANNNNTTC	1 cut(s)	1248
XspI	CTAG	2 cut(s)	602, 1792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.