Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014067

Physical Location & Seq

Genomic Coordinates Reverse (-)

22873375 .. 22876002

Gene Structure 2628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1114100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGTTGAAAGGCATAGCCAAAATAATAACCAGAAAACTGAAGAAATATTGTTTATTGCTCTCTGTCTATTTAAATTCCTTATTTGTCCGTTTCCGGTTCCACGCTCTAAAGCCCAAGCATTTCCCGATGGCGCCCATCGACCCTCACTCGTTCACTGACTCGACTCACCCACTCGCCACCCATATTTCCCTCTCCTTCTTCTTCGACTTCCCCTCCTCCACCATCCACGCCTCCGCCCTCCTCACCCTCCCCGCCCCGTACTCCGGCCCACTCTCCCTCGACATCCGTGCCCTCACCGTCCACTCCGTCATCGACCCCCATAAACCCTCCCGTCCTCTCTCTTACGTCATCTCCCATCCTGACCCAATCAAAGGCTGCCACCTCACCTTCACCCTCTCCAACGCAGCCTCCGTCTTAATACTGTACTCCACCTCCCCCTCCTCCTCCGCCCTTCAGTGGCTCTCCTCTCCCCAGACCTTTAACAAGACGCTGCCGTTTGTCTACACCCAATGCCAGGCCATCCACGCGCGCTCCGTCTTCCCTTGCCAGGACACTCCCGCTGCGCGTGTACGCTACGCGGCCAAGCTCAACGTCCCCCGCCAGCTCTCCGCCGTCATGTCCGCGCGTCACGTCTCCCGCCGCGACCCGATCGCCGGCGAGGGGTCGGCGCTGGTCTCCGGCGACGCCTTGTGGTGCTCTGAGGACAGGGTCGTCGAGGAGTTCGTGATGGAGCAGCCAATCCCGCCGTACCTATTCGCGTTCGCCGTCGGTGAGCTGGGGTTCCGGGAGGTGGGCCCGAGGACTAGGGTTTACTCTGAGGCGGTGCCGGCTGTGCTTGAATCGGCGGCGACGGAGTTCGCGAGCAGCGAGGACATGATCAGGCAAGGGGAGGCCTTGTTTGGGCCGTACGTGTGGGAAAGGTTCGATCTTTTGGTGCTGCCCCCGAGCTTTCCGTACGGTGGAATGGAGAATCCGAGAATGACGTTCTTGACGCCGACGGTGCTCAAAGGCGACGCCAGCGGCGCGCAGGTGGTGGCGCACGAGCTGGCTCATAGCTGGACTGGGAACTTGATCACCAACAAAACTAACGAGCATTTTTGGTTGAATGAGGGTTTCACGACATATGCTGAGAGGAGAATTGTTGAGGCTGTGCAAGGCGAGGACAGAGCTGCACTGAACATTGGAATCGGCTGGCGGGGTTTGCACAAGGAAATGGAGAGGTTCAAGGATAGCTTGGAGGTCACAAAGCTGAAAACCAACCAGGAAGGAGTTGATCCAGATGATGTGTTTTCTCGAGTCCCTTATGAAAAGGGTTTCCAGTTTCTATGGCGCATCGAACGTCAGGTTGGAAGGCCTGCGTTTGATGAGTTTCTGAAGAAATACATTGCCACCTTCAAGTTCCAGTCAATTGATACCGATACGTTTCTCGAGTTTCTCAAGGCAAACCTACCTGGAATAGAGAAAGAGGTTGACTTAGTGGTGTGGACTGAGGGCACTGGTATCCCTCCTGATGCGTATGAACCGGTTTCTAACATATATGCAAAGATTGTGTCCATGGCAAATGAATTTAAGCTTGGTAGGATGCCGGGGGAGGATGAAGTTGCTGACTGGAGAGGGCAGGAGTGGGAACTCTACTTGGAGAGCCTCCCCAAGCCTATTGAACCTTCACAGGTTGTAGAGTTGAACTCGCGCTTCAGGCTGGCAGAATCAAAAGATTATGATGTGAAGGTGACATTTCTCCTGCTGGCCATTGCTTCCAACTGCAAAGACTACTTGGGCGAGGTGGAGAAAACTTTGAAGGAAGTTGGGAGGATGAAGTACCTTGTCCCCCTCTACAGGGCTCTTGTGGAAGGCAATGGAAATGAAGAAGAGAAGATTCTGGCCAAGCAGGTGTTTGCAGCCGCTCGCGAGACTTACCACCCTATAGCTCAAGGCGGTATTGAGACAATCTTAAACAAGCACTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0004301 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006508 GO:0006518 GO:0006629 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0008270 GO:0009056 GO:0009987 GO:0016787 GO:0016801 GO:0016803 GO:0019538 GO:0033218 GO:0034641 GO:0042277 GO:0043167 GO:0043169 GO:0043170 GO:0043171 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0070006 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00590 ko01100 map00590 map01100

Pfam Domains

Protein Families

Leuk-A4-hydro_C Peptidase_M1

Protein Analysis

656

Amino Acids

73.63

Weight (kDa)

6.17

Isoelectric Point (pI)

36.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M1_N	PF17900	160 - 205	1.5e-09	Peptidase M1 N-terminal domain
Peptidase_M1	PF01433	297 - 482	5.6e-43	Peptidase family M1 domain
Leuk-A4-hydro_C	PF09127	537 - 651	7.4e-25	Leukotriene A4 hydrolase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13520
fragaria_vesca	FvH4_7g20511
malus_domestica	MD01G1113900.v1.1 MD01G1114100.v1.1 MD07G1182000.v1.1
prunus_persica	Prupe.2G220700_v2.0.a1
pyrus_communis	pycom01g14270 pycom01g14300
rosa_chinensis	RchiOBHm_Chr1g0364401
rosa_laevigata	RLG00000027483
rosa_multiflora	Rmu_sc0001342.1_g000003
rosa_roxburghii	Rroxscaffold_4G00292310
rosa_rugosa	Rorug01G0316000
rosa_samantha	Rh1BG286800 Rh1CG303400 Rh1DG318300
rosa_wichuraiana	Rw1G028720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1018, 1879
AasI	GACNNNNNNGTC	1 cut(s)	710
Acc36I	ACCTGC	2 cut(s)	1018, 1879
AccB1I	GGYRCC	2 cut(s)	130, 823
AccBSI	CCGCTC	1 cut(s)	1904
AccI	GTMKAC	1 cut(s)	501
AclWI	GGATC	1 cut(s)	1268
AcoI	YGGCCR	3 cut(s)	579, 1746, 1881
AcsI	RAATTY	2 cut(s)	73, 1565
AcuI	CTGAAG	4 cut(s)	59, 437, 1394, 1678
AcyI	GRCGYC	4 cut(s)	131, 684, 992, 1014
AfaI	GTAC	7 cut(s)	260, 425, 570, 749, 908, 956, 1820
AflIII	ACRYGT	1 cut(s)	909
AgeI	ACCGGT	1 cut(s)	1522
AgsI	TTSAA	8 cut(s)	7, 839, 1105, 1225, 1396, 1661, 1684, 1798
AjiI	CACGTC	1 cut(s)	631
AjnI	CCWGG	4 cut(s)	513, 546, 1260, 1450
AjuI	GAANNNNNNNTTGG	4 cut(s)	1329, 1361, 1557, 1589
Alw21I	GWGCWC	2 cut(s)	698, 1005
Alw26I	GTCTC	4 cut(s)	637, 679, 1904, 1937
AlwI	GGATC	1 cut(s)	1268
Ama87I	CYCGRG	4 cut(s)	796, 943, 1293, 1427
AoxI	GGCC	9 cut(s)	265, 516, 579, 793, 891, 902, 1352, 1746, 1881
ApaI	GGGCCC	1 cut(s)	797
ApeKI	GCWGC	9 cut(s)	375, 404, 490, 560, 733, 864, 937, 1169, 1898
ApoI	RAATTY	2 cut(s)	73, 1565
ArsI	GACNNNNNNTTYG	2 cut(s)	1535, 1567
AsiGI	ACCGGT	1 cut(s)	1522
AspS9I	GGNCC	4 cut(s)	266, 793, 794, 902
AsuC2I	CCSGG	2 cut(s)	785, 1587
AsuHPI	GGTGA	8 cut(s)	158, 235, 286, 376, 382, 782, 1066, 1741
AvaI	CYCGRG	4 cut(s)	796, 943, 1293, 1427
BaeGI	GKGCMC	3 cut(s)	292, 797, 1496
BalI	TGGCCA	2 cut(s)	1748, 1883
BanI	GGYRCC	2 cut(s)	130, 823
BanII	GRGCYC	2 cut(s)	797, 1843
BauI	CACGAG	1 cut(s)	1040
BbsI	GAAGAC	1 cut(s)	529
Bbv12I	GWGCWC	2 cut(s)	698, 1005
BbvI	GCAGC	9 cut(s)	362, 416, 477, 547, 745, 876, 924, 1156, 1910
BccI	CCATC	6 cut(s)	121, 143, 230, 363, 527, 721
BceAI	ACGGC	5 cut(s)	478, 596, 730, 749, 889
BcgI	CGANNNNNNTGC	2 cut(s)	269, 303
BciT130I	CCWGG	4 cut(s)	515, 548, 1262, 1452
BciVI	GTATCC	1 cut(s)	1511
BclI	TGATCA	2 cut(s)	876, 1071
BcnI	CCSGG	2 cut(s)	785, 1587
BcoDI	GTCTC	4 cut(s)	637, 679, 1904, 1937
BfaI	CTAG	1 cut(s)	804
BfmI	CTRYAG	2 cut(s)	1834, 1923
BfoI	RGCGCY	2 cut(s)	134, 671
BfuAI	ACCTGC	2 cut(s)	1018, 1879
BfuI	GTATCC	1 cut(s)	1511
Bme1390I	CCNGG	6 cut(s)	515, 548, 785, 1262, 1452, 1587
BmeT110I	CYCGRG	4 cut(s)	796, 943, 1293, 1427
BmgBI	CACGTC	1 cut(s)	631
BmgT120I	GGNCC	4 cut(s)	266, 793, 794, 902
BmiI	GGNNCC	5 cut(s)	98, 132, 782, 795, 825
BmrFI	CCNGG	6 cut(s)	515, 548, 785, 1262, 1452, 1587
BmrI	ACTGGG	1 cut(s)	1071
BmsI	GCATC	3 cut(s)	1341, 1501, 1572
BmuI	ACTGGG	1 cut(s)	1071
BpiI	GAAGAC	1 cut(s)	529
BplI	GAGNNNNNCTC	2 cut(s)	1670, 1702
BpmI	CTGGAG	1 cut(s)	1630
BpuEI	CTTGAG	2 cut(s)	1421, 1914
BpuMI	CCSGG	2 cut(s)	785, 1587
BsaAI	YACGTR	1 cut(s)	910
BsaHI	GRCGYC	4 cut(s)	131, 684, 992, 1014
BsaI	GGTCTC	1 cut(s)	679
BsaJI	CCNNGG	3 cut(s)	797, 1554, 1586
BsaWI	WCCGGW	2 cut(s)	93, 1522
Bse118I	RCCGGY	3 cut(s)	653, 826, 1522
Bse1I	ACTGG	5 cut(s)	1066, 1318, 1402, 1501, 1613
Bse3DI	GCAATG	3 cut(s)	1383, 1749, 1861
BseBI	CCWGG	4 cut(s)	515, 548, 1262, 1452
BseDI	CCNNGG	3 cut(s)	797, 1554, 1586
BseGI	GGATG	7 cut(s)	222, 282, 355, 519, 1587, 1600, 1818
BseMI	GCAATG	3 cut(s)	1383, 1749, 1861
BseMII	CTCAG	4 cut(s)	690, 807, 1119, 1479
BseNI	ACTGG	5 cut(s)	1066, 1318, 1402, 1501, 1613
BsePI	GCGCGC	2 cut(s)	527, 1023
BseRI	GAGGAG	7 cut(s)	205, 230, 430, 433, 454, 731, 1147
BseSI	GKGCMC	3 cut(s)	292, 797, 1496
BseXI	GCAGC	9 cut(s)	362, 416, 477, 547, 745, 876, 924, 1156, 1910
BseYI	CCCAGC	1 cut(s)	775
BsgI	GTGCAG	1 cut(s)	1155
Bsh1285I	CGRYCG	1 cut(s)	651
BshFI	GGCC	9 cut(s)	267, 518, 581, 795, 893, 904, 1354, 1748, 1883
BshNI	GGYRCC	2 cut(s)	130, 823
BshTI	ACCGGT	1 cut(s)	1522
BsiEI	CGRYCG	1 cut(s)	651
BsiHKAI	GWGCWC	2 cut(s)	698, 1005
BsiHKCI	CYCGRG	4 cut(s)	796, 943, 1293, 1427
BsiSI	CCGG	8 cut(s)	94, 264, 654, 678, 784, 827, 1523, 1586
BsiWI	CGTACG	2 cut(s)	906, 954
BslFI	GGGAC	3 cut(s)	578, 1283, 1811
BsmAI	GTCTC	4 cut(s)	637, 679, 1904, 1937
BsmBI	CGTCTC	1 cut(s)	637
BsmFI	GGGAC	3 cut(s)	578, 1283, 1811
BsnI	GGCC	9 cut(s)	267, 518, 581, 795, 893, 904, 1354, 1748, 1883
Bso31I	GGTCTC	1 cut(s)	679
BsoBI	CYCGRG	4 cut(s)	796, 943, 1293, 1427
Bsp120I	GGGCCC	1 cut(s)	793
Bsp1286I	GDGCHC	6 cut(s)	292, 698, 797, 1005, 1496, 1843
Bsp143I	GATC	5 cut(s)	648, 876, 925, 1071, 1273
Bsp19I	CCATGG	1 cut(s)	1554
Bsp68I	TCGCGA	2 cut(s)	860, 1908
BspANI	GGCC	9 cut(s)	267, 518, 581, 795, 893, 904, 1354, 1748, 1883
BspCNI	CTCAG	4 cut(s)	691, 808, 1120, 1480
BspLI	GGNNCC	5 cut(s)	98, 132, 782, 795, 825
BspMI	ACCTGC	2 cut(s)	1018, 1879
BspPI	GGATC	1 cut(s)	1268
BspT107I	GGYRCC	2 cut(s)	130, 823
BspTNI	GGTCTC	1 cut(s)	679
BsrBI	CCGCTC	1 cut(s)	1904
BsrDI	GCAATG	3 cut(s)	1383, 1749, 1861
BsrFI	RCCGGY	3 cut(s)	653, 826, 1522
BsrI	ACTGG	5 cut(s)	1066, 1318, 1402, 1501, 1613
BssAI	RCCGGY	3 cut(s)	653, 826, 1522
BssECI	CCNNGG	3 cut(s)	797, 1554, 1586
BssHII	GCGCGC	2 cut(s)	527, 1023
BssMI	GATC	5 cut(s)	648, 876, 925, 1071, 1273
BssNI	GRCGYC	4 cut(s)	131, 684, 992, 1014
BssSI	CACGAG	1 cut(s)	1040
BssT1I	CCWWGG	1 cut(s)	1554
Bst2BI	CACGAG	1 cut(s)	1040
Bst2UI	CCWGG	4 cut(s)	515, 548, 1262, 1452
Bst4CI	ACNGT	4 cut(s)	298, 423, 959, 1000
Bst6I	CTCTTC	1 cut(s)	1863
BstACI	GRCGYC	4 cut(s)	131, 684, 992, 1014
BstBAI	YACGTR	1 cut(s)	910
BstDEI	CTNAG	5 cut(s)	699, 816, 1128, 1474, 1488
BstDSI	CCRYGG	1 cut(s)	1554
BstF5I	GGATG	7 cut(s)	222, 282, 355, 519, 1587, 1600, 1818
BstH2I	RGCGCY	2 cut(s)	134, 671
BstKTI	GATC	5 cut(s)	651, 879, 928, 1074, 1276
BstMAI	GTCTC	4 cut(s)	637, 679, 1904, 1937
BstMBI	GATC	5 cut(s)	648, 876, 925, 1071, 1273
BstMCI	CGRYCG	1 cut(s)	651
BstMWI	GCNNNNNNNGC	9 cut(s)	524, 742, 827, 832, 1000, 1017, 1022, 1201, 1696
BstNI	CCWGG	4 cut(s)	515, 548, 1262, 1452
BstSCI	CCNGG	6 cut(s)	513, 546, 783, 1260, 1450, 1585
BstSFI	CTRYAG	2 cut(s)	1834, 1923
BstSLI	GKGCMC	3 cut(s)	292, 797, 1496
BstV1I	GCAGC	9 cut(s)	362, 416, 477, 547, 745, 876, 924, 1156, 1910
BstV2I	GAAGAC	1 cut(s)	529
BsuI	GTATCC	1 cut(s)	1511
BsuRI	GGCC	9 cut(s)	267, 518, 581, 795, 893, 904, 1354, 1748, 1883
BtgI	CCRYGG	1 cut(s)	1554
BtrI	CACGTC	1 cut(s)	631
BtsCI	GGATG	7 cut(s)	222, 282, 355, 519, 1587, 1600, 1818
BtsI	GCAGTG	1 cut(s)	1960
BtsIMutI	CAGTG	5 cut(s)	153, 461, 1172, 1494, 1960
BtuMI	TCGCGA	2 cut(s)	860, 1908
BveI	ACCTGC	2 cut(s)	1018, 1879
Cfr10I	RCCGGY	3 cut(s)	653, 826, 1522
Cfr13I	GGNCC	4 cut(s)	266, 793, 794, 902
CseI	GACGC	5 cut(s)	496, 614, 692, 1000, 1022
Csp6I	GTAC	7 cut(s)	259, 424, 569, 748, 907, 955, 1819
CspAI	ACCGGT	1 cut(s)	1522
CviAII	CATG	3 cut(s)	616, 874, 1555
CviQI	GTAC	7 cut(s)	259, 424, 569, 748, 907, 955, 1819
DdeI	CTNAG	5 cut(s)	699, 816, 1128, 1474, 1488
DinI	GGCGCC	1 cut(s)	132
DpnI	GATC	5 cut(s)	650, 878, 927, 1073, 1275
DpnII	GATC	5 cut(s)	648, 876, 925, 1071, 1273
DraI	TTTAAA	1 cut(s)	72
DrdI	GACNNNNNNGTC	1 cut(s)	710
DseDI	GACNNNNNNGTC	1 cut(s)	710
EaeI	YGGCCR	3 cut(s)	579, 1746, 1881
Eam1104I	CTCTTC	1 cut(s)	1863
EarI	CTCTTC	1 cut(s)	1863
EciI	GGCGGA	3 cut(s)	223, 436, 598
Eco130I	CCWWGG	1 cut(s)	1554
Eco147I	AGGCCT	2 cut(s)	893, 1354
Eco24I	GRGCYC	2 cut(s)	797, 1843
Eco31I	GGTCTC	1 cut(s)	679
Eco57I	CTGAAG	4 cut(s)	59, 437, 1394, 1678
Eco88I	CYCGRG	4 cut(s)	796, 943, 1293, 1427
EcoRII	CCWGG	4 cut(s)	513, 546, 1260, 1450
EcoT14I	CCWWGG	1 cut(s)	1554
EcoT38I	GRGCYC	2 cut(s)	797, 1843
EgeI	GGCGCC	1 cut(s)	132
EheI	GGCGCC	1 cut(s)	132
ErhI	CCWWGG	1 cut(s)	1554
Esp3I	CGTCTC	1 cut(s)	637
FaeI	CATG	3 cut(s)	619, 877, 1558
FalI	AAGNNNNNCTT	2 cut(s)	1217, 1249
FaqI	GGGAC	3 cut(s)	578, 1283, 1811
FatI	CATG	3 cut(s)	615, 873, 1554
FauI	CCCGC	6 cut(s)	259, 565, 605, 644, 750, 1188
FauNDI	CATATG	1 cut(s)	1123
FbaI	TGATCA	2 cut(s)	876, 1071
FblI	GTMKAC	1 cut(s)	501
FokI	GGATG	7 cut(s)	209, 269, 342, 506, 1594, 1607, 1825
FriOI	GRGCYC	2 cut(s)	797, 1843
FspBI	CTAG	1 cut(s)	804
GsaI	CCCAGC	1 cut(s)	779
GsuI	CTGGAG	1 cut(s)	1630
HaeII	RGCGCY	2 cut(s)	134, 671
HaeIII	GGCC	9 cut(s)	267, 518, 581, 795, 893, 904, 1354, 1748, 1883
HapII	CCGG	8 cut(s)	94, 264, 654, 678, 784, 827, 1523, 1586
HgaI	GACGC	5 cut(s)	496, 614, 692, 1000, 1022
Hin1I	GRCGYC	4 cut(s)	131, 684, 992, 1014
Hin1II	CATG	3 cut(s)	619, 877, 1558
HincII	GTYRAC	1 cut(s)	1471
HindII	GTYRAC	1 cut(s)	1471
HindIII	AAGCTT	1 cut(s)	1571
HinfI	GANTC	8 cut(s)	158, 163, 839, 970, 1185, 1296, 1706, 1876
HpaII	CCGG	8 cut(s)	94, 264, 654, 678, 784, 827, 1523, 1586
HphI	GGTGA	8 cut(s)	158, 235, 286, 376, 382, 782, 1066, 1741
Hpy166II	GTNNAC	7 cut(s)	153, 301, 502, 569, 811, 1471, 1485
Hpy188I	TCNGA	4 cut(s)	700, 817, 975, 1374
Hpy8I	GTNNAC	7 cut(s)	153, 301, 502, 569, 811, 1471, 1485
Hpy99I	CGWCG	6 cut(s)	686, 716, 770, 853, 1000, 1016
HpyCH4III	ACNGT	4 cut(s)	298, 423, 959, 1000
HpyCH4IV	ACGT	7 cut(s)	345, 591, 630, 909, 983, 1339, 1421
HpyCH4V	TGCA	6 cut(s)	1153, 1172, 1204, 1541, 1764, 1898
HpyF10VI	GCNNNNNNNGC	9 cut(s)	524, 742, 827, 832, 1000, 1017, 1022, 1201, 1696
HpyF3I	CTNAG	5 cut(s)	699, 816, 1128, 1474, 1488
HpySE526I	ACGT	7 cut(s)	345, 591, 630, 909, 983, 1339, 1421
Hsp92I	GRCGYC	4 cut(s)	131, 684, 992, 1014
Hsp92II	CATG	3 cut(s)	619, 877, 1558
KasI	GGCGCC	1 cut(s)	130
KroI	GCCGGC	2 cut(s)	653, 826
KroNI	GCCGGC	2 cut(s)	655, 828
Ksp22I	TGATCA	2 cut(s)	876, 1071
Kzo9I	GATC	5 cut(s)	648, 876, 925, 1071, 1273
LmnI	GCTCC	2 cut(s)	536, 730
Lsp1109I	GCAGC	9 cut(s)	362, 416, 477, 547, 745, 876, 924, 1156, 1910
LweI	GCATC	3 cut(s)	1341, 1501, 1572
MaeI	CTAG	1 cut(s)	804
MaeII	ACGT	7 cut(s)	345, 591, 630, 909, 983, 1339, 1421
MaeIII	GTNAC	3 cut(s)	626, 1240, 1729
MalI	GATC	5 cut(s)	650, 878, 927, 1073, 1275
MbiI	CCGCTC	1 cut(s)	1904
MboI	GATC	5 cut(s)	648, 876, 925, 1071, 1273
MboII	GAAGA	8 cut(s)	52, 190, 193, 529, 1387, 1877, 1880, 1885
MfeI	CAATTG	1 cut(s)	1407
MhlI	GDGCHC	6 cut(s)	292, 698, 797, 1005, 1496, 1843
MlsI	TGGCCA	2 cut(s)	1748, 1883
MluCI	AATT	4 cut(s)	73, 1137, 1407, 1565
MluNI	TGGCCA	2 cut(s)	1748, 1883
Mly113I	GGCGCC	1 cut(s)	131
MlyI	GAGTC	3 cut(s)	152, 157, 1305
MmeI	TCCRAC	3 cut(s)	423, 1327, 1782
Mox20I	TGGCCA	2 cut(s)	1748, 1883
MreI	CGCCGGCG	1 cut(s)	653
MroNI	GCCGGC	2 cut(s)	653, 826
MscI	TGGCCA	2 cut(s)	1748, 1883
MseI	TTAA	5 cut(s)	71, 416, 480, 1569, 1952
Msp20I	TGGCCA	2 cut(s)	1748, 1883
MspA1I	CMGCKG	2 cut(s)	560, 1020
MspI	CCGG	8 cut(s)	94, 264, 654, 678, 784, 827, 1523, 1586
MspR9I	CCNGG	6 cut(s)	515, 548, 785, 1262, 1452, 1587
MunI	CAATTG	1 cut(s)	1407
MvaI	CCWGG	4 cut(s)	515, 548, 1262, 1452
MwoI	GCNNNNNNNGC	9 cut(s)	524, 742, 827, 832, 1000, 1017, 1022, 1201, 1696
NaeI	GCCGGC	2 cut(s)	655, 828
NarI	GGCGCC	1 cut(s)	131
NciI	CCSGG	2 cut(s)	785, 1587
NcoI	CCATGG	1 cut(s)	1554
NdeI	CATATG	1 cut(s)	1123
NdeII	GATC	5 cut(s)	648, 876, 925, 1071, 1273
NgoMIV	GCCGGC	2 cut(s)	653, 826
NlaIII	CATG	3 cut(s)	619, 877, 1558
NlaIV	GGNNCC	5 cut(s)	98, 132, 782, 795, 825
NmuCI	GTSAC	3 cut(s)	626, 1240, 1729
NruI	TCGCGA	2 cut(s)	860, 1908
PaeR7I	CTCGAG	2 cut(s)	1293, 1427
PaqCI	CACCTGC	2 cut(s)	1018, 1879
PauI	GCGCGC	2 cut(s)	527, 1023
PceI	AGGCCT	2 cut(s)	893, 1354
PcsI	WCGNNNNNNNCGW	6 cut(s)	408, 531, 720, 762, 857, 864
PdiI	GCCGGC	2 cut(s)	655, 828
PfeI	GAWTC	5 cut(s)	839, 970, 1185, 1706, 1876
Pfl23II	CGTACG	2 cut(s)	906, 954
PflFI	GACNNNGTC	1 cut(s)	707
PfoI	TCCNGGA	1 cut(s)	783
PinAI	ACCGGT	1 cut(s)	1522
Ple19I	CGATCG	1 cut(s)	651
PleI	GAGTC	3 cut(s)	152, 157, 1304
PluTI	GGCGCC	1 cut(s)	134
PpsI	GAGTC	3 cut(s)	152, 157, 1304
Ppu21I	YACGTR	1 cut(s)	910
Psp6I	CCWGG	4 cut(s)	513, 546, 1260, 1450
PspFI	CCCAGC	1 cut(s)	775
PspGI	CCWGG	4 cut(s)	513, 546, 1260, 1450
PspLI	CGTACG	2 cut(s)	906, 954
PspN4I	GGNNCC	5 cut(s)	98, 132, 782, 795, 825
PspOMI	GGGCCC	1 cut(s)	793
PspPI	GGNCC	4 cut(s)	266, 793, 794, 902
PsyI	GACNNNGTC	1 cut(s)	707
PteI	GCGCGC	2 cut(s)	527, 1023
PvuI	CGATCG	1 cut(s)	651
RruI	TCGCGA	2 cut(s)	860, 1908
RsaI	GTAC	7 cut(s)	260, 425, 570, 749, 908, 956, 1820
RsaNI	GTAC	7 cut(s)	259, 424, 569, 748, 907, 955, 1819
SaqAI	TTAA	5 cut(s)	71, 416, 480, 1569, 1952
Sau3AI	GATC	5 cut(s)	648, 876, 925, 1071, 1273
Sau96I	GGNCC	4 cut(s)	266, 793, 794, 902
SchI	GAGTC	3 cut(s)	152, 157, 1305
ScrFI	CCNGG	6 cut(s)	515, 548, 785, 1262, 1452, 1587
SduI	GDGCHC	6 cut(s)	292, 698, 797, 1005, 1496, 1843
SfaNI	GCATC	3 cut(s)	1341, 1501, 1572
SfcI	CTRYAG	2 cut(s)	1834, 1923
SfoI	GGCGCC	1 cut(s)	132
Sfr274I	CTCGAG	2 cut(s)	1293, 1427
SgrAI	CRCCGGYG	1 cut(s)	653
SlaI	CTCGAG	2 cut(s)	1293, 1427
SmiI	ATTTAAAT	1 cut(s)	72
SmlI	CTYRAG	4 cut(s)	1293, 1427, 1436, 1929
SmoI	CTYRAG	4 cut(s)	1293, 1427, 1436, 1929
Sse9I	AATT	4 cut(s)	73, 1137, 1407, 1565
SseBI	AGGCCT	2 cut(s)	893, 1354
SspDI	GGCGCC	1 cut(s)	130
SspI	AATATT	1 cut(s)	47
SspMI	CTAG	1 cut(s)	804
StuI	AGGCCT	2 cut(s)	893, 1354
StyD4I	CCNGG	6 cut(s)	513, 546, 783, 1260, 1450, 1585
StyI	CCWWGG	1 cut(s)	1554
SwaI	ATTTAAAT	1 cut(s)	72
TaaI	ACNGT	4 cut(s)	298, 423, 959, 1000
TaiI	ACGT	7 cut(s)	348, 594, 633, 912, 986, 1342, 1424
TasI	AATT	4 cut(s)	73, 1137, 1407, 1565
TatI	WGTACW	1 cut(s)	423
TauI	GCSGC	5 cut(s)	581, 642, 848, 1023, 1904
TfiI	GAWTC	5 cut(s)	839, 970, 1185, 1706, 1876
Tru1I	TTAA	5 cut(s)	71, 416, 480, 1569, 1952
Tru9I	TTAA	5 cut(s)	71, 416, 480, 1569, 1952
TscAI	CASTG	5 cut(s)	160, 461, 1179, 1501, 1967
TseFI	GTSAC	3 cut(s)	626, 1240, 1729
TseI	GCWGC	9 cut(s)	375, 404, 490, 560, 733, 864, 937, 1169, 1898
Tsp45I	GTSAC	3 cut(s)	626, 1240, 1729
TspDTI	ATGAA	6 cut(s)	1320, 1533, 1578, 1611, 1829, 1878
TspGWI	ACGGA	7 cut(s)	77, 275, 295, 400, 523, 866, 942
TspRI	CASTG	5 cut(s)	160, 461, 1179, 1501, 1967
Tth111I	GACNNNGTC	1 cut(s)	707
XapI	RAATTY	2 cut(s)	73, 1565
XhoI	CTCGAG	2 cut(s)	1293, 1427
XmiI	GTMKAC	1 cut(s)	501
XspI	CTAG	1 cut(s)	804

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.