Rorug01G0316000

Belongs to the adenylate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012875
Physical Location & Seq
Forward (+)
43368196 .. 43373289
5094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0316000.1

Sequence Viewer

Length: 3156 bp
ATGGACGAAAAGCCATTCCCGGCATGGTCATGGTCAGTTGATCAGTGTTTGAAAGAGCATCATGTAAAACTTGAGAAGGGTCTGGGCACTTATGAGGCTGAGAAGCGGCGTGAAAGGTATGGGTGGAATGAGCTTACCAAAGAGAAAGCGAAGCCCTTATGGCGGCTTGTATTAGAGCAGTTTGATGACACTCTTGTGAAGATACTTCTAGTTGCAGCCTTTATCTCGTTCGCGTTAGCTTTCATGCATGGAAGTGAGTCAGGGGAGTCGGGGTTTGAGGCCTATGTGGAACCATTTGTGATAGTCTTGATTCTAGTGCTTAATGCCATTGTTGGTGTTTGGCAAGAGAGCAATGCTGAAAAAGCACTTGAAGCTCTTAAACAGATGCAAAGTGAATCTGCAAAGGTGCTAAGGGATGGGTATTTGGTGCCGGATTTGCCTGCAAGAGAACTTGTTCCTGGCGATATTGTGGAGTTGCGAGTTGGGGACAAAGTCCCTGCTGACATGAGAGTTGCAGTTTTGAAAACCTCAACTTTGCGAGTTGAGCAGAGCTCATTGACCGGAGAGGCAATGCCTGTTTTGAAGAGCACTGATTCTATACTCATGGATGACAGTGACCTGCAAGCTAAAGAGAGTATGGTTTTTGCAGGCACAACAGTTGTAAATGGTAGCTGTCTTTGTATCGTTGTTAGCACTGGGATGAACACCGAGATTGGCAAAATACAAAAGCAAATACATGATGCTTCTTTGGAAGAAGATGACACCCCTTTGAAGAAGAAGCTGGATGAGTTTGGAAGCAAGTTTACTACAGCAATTGGGTTCGTTTGCCTCATTGTTTGGGTTATGAACTACAAAAACTTCATTTCATGGGATATGAAAGATGGATGGCCAGTAAATGTGCGGTTTTCTTTCGAGAGATGCACTTACTATTTTAAGATAGCTGTTGCACTTGCAGTAGCTGCAATCCCAGAAGGTCTCCCTGCTGTTATTACAACTTGCTTAGCTCTCGGGACAAGGAAGATGGCACAAAAAAATGCAATTGTGAGGAAGCTTCCGAGTGTAGAAACTTTAGGATGTACTAGCGTGATTTGCTCAGATAAAACTGGGACTTTGACAACAAATCAGATGTCTGTGACAGAATTCTTCACTTTGGGTGGGAAAACTACAGCTTCTCGAACGATTCATGTTCAAGGCACAACTTATGATCCCAAGGATGGGGGAATTGTTGACTGGACTTGCTTCAATATGGATGCAAATATGCAAGCCATGGCAGAAATATGTGCCGTTTGCAATGATGCTGGGATTTATTTTGATGGTCAACTCTTTCGGGCTACAGGTTTGCCAACTGAGGCAGCTCTTAAGGTTTTGGTTGAAAAGATGGGAGTTCCAGACGTCAAGGCTAGGAACAAAATTCGTGACTCACAGCTTGCTGCAAGCTACTTAATTGACTCCACTTCAGTGAAATTAGGCTGTTGTGAGTGGTGGACAAAAAGATCAAAAAGGGTTGCCACACTAGAGTTTGATCGTGTTCGAAAGTCAATGAGTGTTATTACTCGGGGGCCAACTGGGCATAATCGACTTCTTGTCAAGGGTGCTGTTGAGAGTTTACTGGAGCGAACTTCGCATGTGCAACTTGCAGATGGATCCCTTGTTCCAATAGATGAACCTTGTAAACAATCATTGCTTTTGAGACTCTTGGAGATGAGCTCAAAGGGTTTGAGGTGCTTGGGTTTTGCATATAAAGATGACTTGGGAAAGCTCTCAGATTATTCTTCTGGGAGTCATCCAGCTCACAAGATGTTGCAGGATCCAGCCTGCTACTCCTCCATCGAAAGTGACTTAGTTTTTGTAGGAATTGTTGGCTTAAGAGACCCTCCTCGTGATGAAGTTAGCAAAGCAATCGAAGATTGTAAAGAAGCTGGAATTAGAGTTATGGTAATAACCGGAGATAATAAGTCTACAGCTGAAGCTATCTGTCAAGAAATCAAATTGTTTTCGAGAAATGAGGATCTGAAAGGGAGAAGCTTTACAGGAAAAGAGTTTATGGCTCTTTCACCATCACAACAAATGGAAACTTTATCAAAACCAGGAGGGAAGGTTTTCTCTCGTGCCGAGCCTAGACATAAGCAAGAAATAGTAAGGATGCTGAAAGAGATGGGGGAGATTGTTGCAATGACTGGAGATGGAGTGAATGATGCACCAGCACTTAAACTTGCTGACATTGGAATCGCCATGGGCATTACTGGAACTGAGGTAGCAAAAGAAGCTTCAGATATGGTTTTGGCTGATGATAATTTCAGTACTATTGTTTCAGCTGTTGCAGAGGGCCGTGCCATTTACACTAACATGAAAGCTTTTATCAGGTACATGATATCTTCAAATGTTGGAGAGGTAATATCCATATTCTTGACTGCGGCATTGGGAATACCTGAGTGTATGATACCTGTGCAGCTTCTGTGGGTAAATTTGGTTACAGATGGACCACCTGCAACAGCTCTTGGCTTTAATCCAGCTGACATTCACATTATGAAGAAACCACCACGCAAAAGCAAAGAAGCTCTTATGAATTCTTGGGTTCTTCTCCGTTACTTGGTAATTGGTTCTTATGTGGGCATTGCAACTGTTGGAGTCTTCATCTTGTGGTACACTCAGGCTTCATTCATGGGTATCAATCTTGTGAGTGATGGACATACACTTGTTAAATTATCTCAGCTTCGCAATTGGGGAGAGTGTTCCTCGTGGTCAAATTTCACAGCGGCTCCATTCATGGTTAATGGGGGCAGGATGATCGCTTTTACTGACCCTTGTGACTATTTTTCTGTTGGTAAAGTGAAGGCAATGACTTTGTCACTCTCTGTCTTGGTGGCAATTGAGATGTTCAATTCCCTCAATGCTCTTTCTGAAGACATTAGCTTGCTCAAAATGCCTCCTTGGAAGAACCCTTGGCTTTTGGTCGCCATGTCAGTCTCATTCGGACTCCATTGCGTCATTCTCTACATTCCATTTTTAGCAGATGTGTTTGGTGTTGTCCCATTGAATCTGAACGAATGGCTTCTGGTAGTTTTGATTTCAGCACCGGTGATTCTTATCGATGAGGTTCTTAAGTTAGTAGGAAGTAGTAGACGATGGAGAGCAAAGAAGGAGAAGACAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 ko01110 ko01130 map00230 map00730 map01100 map01110 map01130
Pfam Domains
Protein Families
ADK

Protein Analysis

1051

Amino Acids

115.64

Weight (kDa)

6.22

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 9 - 76 3.7e-19 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 129 - 245 1.7e-26 P-type ATPase actuator domain
Cation_ATPase PF13246 420 - 629 2.7e-39 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 617 - 740 1.2e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 811 - 1037 5.1e-47 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39270
fragaria_vesca FvH4_7g20530
malus_domestica MD01G1114300.v1.1 MD07G1182200.v1.1
prunus_persica Prupe.2G220900_v2.0.a1
pyrus_communis pycom01g14330 pycom07g17490
rosa_chinensis RchiOBHm_Chr1g0364421
rosa_laevigata RLG00000027481
rosa_multiflora Rmu_sc0001342.1_g000005
rosa_roxburghii Rroxscaffold_4G00292290
rosa_rugosa Rorug01G0316000 Rorug01G0316000
rosa_samantha Rh1AG324300 Rh1BG287000 Rh1CG303600 Rh1DG318500
rosa_wichuraiana Rw1G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2494
AatII GACGTC 1 cut(s) 1395
Acc36I ACCTGC 2 cut(s) 627, 2494
AccB1I GGYRCC 1 cut(s) 427
AccI GTMKAC 2 cut(s) 1958, 3121
AccII CGCG 1 cut(s) 233
AciI CCGC 5 cut(s) 106, 163, 901, 2414, 2756
AclWI GGATC 6 cut(s) 1199, 1638, 1651, 1802, 1815, 2016
AcoI YGGCCR 1 cut(s) 887
AcsI RAATTY 5 cut(s) 1139, 1410, 2464, 2566, 2746
AcuI CTGAAG 4 cut(s) 1440, 1986, 2253, 2922
AcyI GRCGYC 1 cut(s) 1392
AfaI GTAC 4 cut(s) 1078, 2302, 2366, 2645
AfiI CCNNNNNNNGG 4 cut(s) 162, 1214, 1215, 2590
AflII CTTAAG 3 cut(s) 1358, 1864, 3101
AgeI ACCGGT 1 cut(s) 3076
AhdI GACNNNNNGTC 1 cut(s) 1583
AjnI CCWGG 2 cut(s) 457, 2086
AleI CACNNNNGTG 2 cut(s) 194, 1457
AloI GAACNNNNNNTCC 2 cut(s) 1635, 1667
Alw21I GWGCWC 3 cut(s) 554, 590, 1709
Alw26I GTCTC 4 cut(s) 980, 1684, 1863, 2971
AlwI GGATC 6 cut(s) 1199, 1638, 1651, 1802, 1815, 2016
AlwNI CAGNNNCTG 2 cut(s) 959, 2455
Ama87I CYCGRG 2 cut(s) 1007, 1554
AoxI GGCC 4 cut(s) 279, 887, 1559, 2326
ApeKI GCWGC 5 cut(s) 215, 959, 1352, 1430, 2449
ApoI RAATTY 5 cut(s) 1139, 1410, 2464, 2566, 2746
ArsI GACNNNNNNTTYG 4 cut(s) 1112, 1144, 1829, 1861
AsiGI ACCGGT 1 cut(s) 3076
Asp700I GAANNNNTTC 3 cut(s) 453, 2099, 3051
AspS9I GGNCC 3 cut(s) 1559, 2326, 2480
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 2 cut(s) 2046, 3091
AsuII TTCGAA 1 cut(s) 1531
AvaI CYCGRG 2 cut(s) 1007, 1554
AvaII GGWCC 1 cut(s) 2480
BaeGI GKGCMC 1 cut(s) 89
BalI TGGCCA 1 cut(s) 889
BamHI GGATCC 2 cut(s) 1643, 1807
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 554, 1709
BarI GAAGNNNNNNTAC 2 cut(s) 787, 819
BauI CACGAG 3 cut(s) 1878, 2106, 2737
BbsI GAAGAC 3 cut(s) 2623, 2910, 3152
Bbv12I GWGCWC 3 cut(s) 554, 590, 1709
BbvI GCAGC 5 cut(s) 227, 946, 1364, 1417, 2461
BceAI ACGGC 2 cut(s) 1268, 2313
BcgI CGANNNNNNTGC 4 cut(s) 1882, 1916, 2770, 2804
BciT130I CCWGG 2 cut(s) 459, 2088
BclI TGATCA 1 cut(s) 40
BcnI CCSGG 1 cut(s) 20
BcoDI GTCTC 4 cut(s) 980, 1684, 1863, 2971
BfaI CTAG 6 cut(s) 209, 314, 1080, 1401, 1514, 2118
BfmI CTRYAG 4 cut(s) 807, 1164, 1332, 1959
BfrI CTTAAG 3 cut(s) 1358, 1864, 3101
BfuAI ACCTGC 2 cut(s) 627, 2494
BglI GCCNNNNNGGC 2 cut(s) 160, 1567
BisI GCNGC 9 cut(s) 107, 164, 216, 960, 1353, 1431, 2415, 2450, 2757
BlpI GCTNAGC 1 cut(s) 1000
BlsI GCNGC 9 cut(s) 108, 165, 217, 961, 1354, 1432, 2416, 2451, 2758
BmcAI AGTACT 1 cut(s) 2302
Bme1390I CCNGG 3 cut(s) 20, 459, 2088
Bme18I GGWCC 1 cut(s) 2480
BmeRI GACNNNNNGTC 1 cut(s) 1583
BmeT110I CYCGRG 2 cut(s) 1007, 1554
BmgT120I GGNCC 3 cut(s) 1559, 2326, 2480
BmiI GGNNCC 6 cut(s) 291, 429, 1560, 1645, 1809, 2760
BmrFI CCNGG 3 cut(s) 20, 459, 2088
BmrI ACTGGG 3 cut(s) 705, 1113, 1575
BmsI GCATC 8 cut(s) 67, 375, 730, 908, 1240, 1285, 2133, 2185
BmuI ACTGGG 3 cut(s) 705, 1113, 1575
BpiI GAAGAC 3 cut(s) 2623, 2910, 3152
BplI GAGNNNNNCTC 6 cut(s) 536, 568, 1691, 1723, 2720, 2752
BpmI CTGGAG 2 cut(s) 1631, 2199
Bpu10I CCTNAGC 1 cut(s) 410
Bpu1102I GCTNAGC 1 cut(s) 1000
Bpu14I TTCGAA 1 cut(s) 1531
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 20
Bsa29I ATCGAT 1 cut(s) 3090
BsaBI GATNNNNATC 1 cut(s) 3086
BsaHI GRCGYC 1 cut(s) 1392
BsaI GGTCTC 2 cut(s) 980, 1863
BsaJI CCNNGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
BsaWI WCCGGW 3 cut(s) 560, 1943, 3076
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 2082, 2112, 2172, 2202, 2743, 2773
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 1214, 1215, 2590
Bse118I RCCGGY 1 cut(s) 3076
Bse1I ACTGG 8 cut(s) 700, 890, 1108, 1235, 1570, 1614, 2182, 2248
Bse3DI GCAATG 8 cut(s) 358, 576, 1297, 1679, 2178, 2613, 2844, 2980
Bse8I GATNNNNATC 1 cut(s) 3086
BseBI CCWGG 2 cut(s) 459, 2088
BseCI ATCGAT 1 cut(s) 3090
BseDI CCNNGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
BseJI GATNNNNATC 1 cut(s) 3086
BseLI CCNNNNNNNGG 4 cut(s) 162, 1214, 1215, 2590
BseMI GCAATG 8 cut(s) 358, 576, 1297, 1679, 2178, 2613, 2844, 2980
BseMII CTCAG 8 cut(s) 90, 1107, 1338, 1776, 2241, 2421, 2663, 2723
BseNI ACTGG 8 cut(s) 700, 890, 1108, 1235, 1570, 1614, 2182, 2248
BseRI GAGGAG 2 cut(s) 1813, 1866
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 5 cut(s) 227, 946, 1364, 1417, 2461
BseYI CCCAGC 1 cut(s) 1298
BsgI GTGCAG 1 cut(s) 2468
Bsh1236I CGCG 1 cut(s) 233
BshFI GGCC 4 cut(s) 281, 889, 1561, 2328
BshNI GGYRCC 1 cut(s) 427
BshTI ACCGGT 1 cut(s) 3076
BshVI ATCGAT 1 cut(s) 3090
BsiHKAI GWGCWC 3 cut(s) 554, 590, 1709
BsiHKCI CYCGRG 2 cut(s) 1007, 1554
BsiSI CCGG 5 cut(s) 20, 431, 561, 1944, 3077
BslFI GGGAC 5 cut(s) 479, 500, 1024, 1120, 3014
BslI CCNNNNNNNGG 4 cut(s) 162, 1214, 1215, 2590
BsmAI GTCTC 4 cut(s) 980, 1684, 1863, 2971
BsmFI GGGAC 5 cut(s) 479, 500, 1024, 1120, 3014
BsnI GGCC 4 cut(s) 281, 889, 1561, 2328
Bso31I GGTCTC 2 cut(s) 980, 1863
BsoBI CYCGRG 2 cut(s) 1007, 1554
Bsp119I TTCGAA 1 cut(s) 1531
Bsp1286I GDGCHC 4 cut(s) 89, 554, 590, 1709
Bsp143I GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
Bsp1720I GCTNAGC 1 cut(s) 1000
Bsp19I CCATGG 2 cut(s) 1266, 2232
BspACI CCGC 5 cut(s) 106, 163, 901, 2414, 2756
BspANI GGCC 4 cut(s) 281, 889, 1561, 2328
BspCNI CTCAG 8 cut(s) 91, 1106, 1339, 1775, 2242, 2422, 2662, 2722
BspDI ATCGAT 1 cut(s) 3090
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 6 cut(s) 291, 429, 1560, 1645, 1809, 2760
BspMI ACCTGC 2 cut(s) 627, 2494
BspPI GGATC 6 cut(s) 1199, 1638, 1651, 1802, 1815, 2016
BspQI GCTCTTC 1 cut(s) 578
BspT104I TTCGAA 1 cut(s) 1531
BspT107I GGYRCC 1 cut(s) 427
BspTI CTTAAG 3 cut(s) 1358, 1864, 3101
BspTNI GGTCTC 2 cut(s) 980, 1863
BsrDI GCAATG 8 cut(s) 358, 576, 1297, 1679, 2178, 2613, 2844, 2980
BsrFI RCCGGY 1 cut(s) 3076
BsrI ACTGG 8 cut(s) 700, 890, 1108, 1235, 1570, 1614, 2182, 2248
BssAI RCCGGY 1 cut(s) 3076
BssECI CCNNGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
BssMI GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
BssNI GRCGYC 1 cut(s) 1392
BssSI CACGAG 3 cut(s) 1878, 2106, 2737
BssT1I CCWWGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
Bst2BI CACGAG 3 cut(s) 1878, 2106, 2737
Bst2UI CCWGG 2 cut(s) 459, 2088
Bst4CI ACNGT 3 cut(s) 614, 658, 2623
Bst6I CTCTTC 1 cut(s) 578
BstACI GRCGYC 1 cut(s) 1392
BstAFI CTTAAG 3 cut(s) 1358, 1864, 3101
BstAPI GCANNNNNTGC 1 cut(s) 959
BstBI TTCGAA 1 cut(s) 1531
BstC8I GCNNGC 8 cut(s) 441, 624, 649, 1263, 1428, 1435, 1816, 2915
BstDSI CCRYGG 2 cut(s) 1266, 2232
BstFNI CGCG 1 cut(s) 233
BstKTI GATC 8 cut(s) 43, 1207, 1496, 1525, 1646, 1810, 2011, 2790
BstMAI GTCTC 4 cut(s) 980, 1684, 1863, 2971
BstMBI GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
BstNI CCWGG 2 cut(s) 459, 2088
BstNSI RCATGY 1 cut(s) 1628
BstSCI CCNGG 3 cut(s) 18, 457, 2086
BstSFI CTRYAG 4 cut(s) 807, 1164, 1332, 1959
BstSLI GKGCMC 1 cut(s) 89
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 5 cut(s) 227, 946, 1364, 1417, 2461
BstV2I GAAGAC 3 cut(s) 2623, 2910, 3152
BstX2I RGATCY 3 cut(s) 1643, 1807, 2008
BstYI RGATCY 3 cut(s) 1643, 1807, 2008
Bsu15I ATCGAT 1 cut(s) 3090
BsuRI GGCC 4 cut(s) 281, 889, 1561, 2328
BsuTUI ATCGAT 1 cut(s) 3090
BtgI CCRYGG 2 cut(s) 1266, 2232
BtsIMutI CAGTG 5 cut(s) 50, 588, 619, 693, 1464
BveI ACCTGC 2 cut(s) 627, 2494
Cac8I GCNNGC 8 cut(s) 441, 624, 649, 1263, 1428, 1435, 1816, 2915
CaiI CAGNNNCTG 2 cut(s) 959, 2455
Cfr10I RCCGGY 1 cut(s) 3076
Cfr13I GGNCC 3 cut(s) 1559, 2326, 2480
ClaI ATCGAT 1 cut(s) 3090
CseI GACGC 1 cut(s) 2974
Csp6I GTAC 4 cut(s) 1077, 2301, 2365, 2644
CspAI ACCGGT 1 cut(s) 3076
CviQI GTAC 4 cut(s) 1077, 2301, 2365, 2644
DpnI GATC 8 cut(s) 42, 1206, 1495, 1524, 1645, 1809, 2010, 2789
DpnII GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
DriI GACNNNNNGTC 1 cut(s) 1583
EaeI YGGCCR 1 cut(s) 887
Eam1104I CTCTTC 1 cut(s) 578
Eam1105I GACNNNNNGTC 1 cut(s) 1583
EarI CTCTTC 1 cut(s) 578
Ecl136II GAGCTC 2 cut(s) 552, 1707
Eco130I CCWWGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
Eco147I AGGCCT 1 cut(s) 281
Eco24I GRGCYC 2 cut(s) 554, 1709
Eco31I GGTCTC 2 cut(s) 980, 1863
Eco32I GATATC 1 cut(s) 2373
Eco47I GGWCC 1 cut(s) 2480
Eco53kI GAGCTC 2 cut(s) 552, 1707
Eco57I CTGAAG 4 cut(s) 1440, 1986, 2253, 2922
Eco88I CYCGRG 2 cut(s) 1007, 1554
EcoICRI GAGCTC 2 cut(s) 552, 1707
EcoRI GAATTC 2 cut(s) 1139, 2566
EcoRII CCWGG 2 cut(s) 457, 2086
EcoRV GATATC 1 cut(s) 2373
EcoT14I CCWWGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
EcoT22I ATGCAT 1 cut(s) 249
EcoT38I GRGCYC 2 cut(s) 554, 1709
ErhI CCWWGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
FalI AAGNNNNNCTT 2 cut(s) 2544, 2576
FaqI GGGAC 5 cut(s) 479, 500, 1024, 1120, 3014
FbaI TGATCA 1 cut(s) 40
FblI GTMKAC 2 cut(s) 1958, 3121
Fnu4HI GCNGC 9 cut(s) 107, 164, 216, 960, 1353, 1431, 2415, 2450, 2757
FriOI GRGCYC 2 cut(s) 554, 1709
Fsp4HI GCNGC 9 cut(s) 107, 164, 216, 960, 1353, 1431, 2415, 2450, 2757
FspBI CTAG 6 cut(s) 209, 314, 1080, 1401, 1514, 2118
GluI GCNGC 9 cut(s) 107, 164, 216, 960, 1353, 1431, 2415, 2450, 2757
GsaI CCCAGC 1 cut(s) 1302
GsuI CTGGAG 2 cut(s) 1631, 2199
HaeIII GGCC 4 cut(s) 281, 889, 1561, 2328
HapII CCGG 5 cut(s) 20, 431, 561, 1944, 3077
HgaI GACGC 1 cut(s) 2974
Hin1I GRCGYC 1 cut(s) 1392
HincII GTYRAC 2 cut(s) 1228, 1319
HindII GTYRAC 2 cut(s) 1228, 1319
HindIII AAGCTT 4 cut(s) 1049, 2023, 2265, 2352
HpaII CCGG 5 cut(s) 20, 431, 561, 1944, 3077
HphI GGTGA 2 cut(s) 2046, 3091
Hpy166II GTNNAC 9 cut(s) 804, 1228, 1319, 1485, 1607, 1673, 1959, 2646, 3122
Hpy188I TCNGA 9 cut(s) 1056, 1096, 1125, 1765, 2013, 2272, 2902, 2975, 3042
Hpy8I GTNNAC 9 cut(s) 804, 1228, 1319, 1485, 1607, 1673, 1959, 2646, 3122
HpyAV CCTTC 5 cut(s) 70, 965, 2089, 2827, 3133
HpyCH4III ACNGT 3 cut(s) 614, 658, 2623
HpyCH4IV ACGT 1 cut(s) 1392
HpySE526I ACGT 1 cut(s) 1392
Hsp92I GRCGYC 1 cut(s) 1392
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
LguI GCTCTTC 1 cut(s) 578
LmnI GCTCC 2 cut(s) 1612, 2764
Lsp1109I GCAGC 5 cut(s) 227, 946, 1364, 1417, 2461
LweI GCATC 8 cut(s) 67, 375, 730, 908, 1240, 1285, 2133, 2185
MaeI CTAG 6 cut(s) 209, 314, 1080, 1401, 1514, 2118
MaeII ACGT 1 cut(s) 1392
MaeIII GTNAC 8 cut(s) 614, 1132, 1415, 1835, 2470, 2585, 2807, 2847
MalI GATC 8 cut(s) 42, 1206, 1495, 1524, 1645, 1809, 2010, 2789
MboI GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
MfeI CAATTG 4 cut(s) 813, 1038, 2719, 2868
MflI RGATCY 3 cut(s) 1643, 1807, 2008
MhlI GDGCHC 4 cut(s) 89, 554, 590, 1709
MlsI TGGCCA 1 cut(s) 889
MluNI TGGCCA 1 cut(s) 889
MlyI GAGTC 8 cut(s) 266, 275, 1412, 1442, 1686, 1789, 2637, 2970
MmeI TCCRAC 2 cut(s) 2365, 2605
Mox20I TGGCCA 1 cut(s) 889
Mph1103I ATGCAT 1 cut(s) 249
MroXI GAANNNNTTC 3 cut(s) 453, 2099, 3051
MscI TGGCCA 1 cut(s) 889
MslI CAYNNNNRTG 6 cut(s) 28, 194, 252, 698, 1457, 2345
Msp20I TGGCCA 1 cut(s) 889
MspA1I CMGCKG 4 cut(s) 1964, 2315, 2513, 2756
MspCI CTTAAG 3 cut(s) 1358, 1864, 3101
MspI CCGG 5 cut(s) 20, 431, 561, 1944, 3077
MspR9I CCNGG 3 cut(s) 20, 459, 2088
MunI CAATTG 4 cut(s) 813, 1038, 2719, 2868
MvaI CCWGG 2 cut(s) 459, 2088
MvnI CGCG 1 cut(s) 233
NciI CCSGG 1 cut(s) 20
NcoI CCATGG 2 cut(s) 1266, 2232
NdeII GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
NlaIV GGNNCC 6 cut(s) 291, 429, 1560, 1645, 1809, 2760
NmeAIII GCCGAG 1 cut(s) 2137
NmuCI GTSAC 6 cut(s) 614, 1132, 1415, 1835, 2807, 2847
NsiI ATGCAT 1 cut(s) 249
NspI RCATGY 1 cut(s) 1628
NspV TTCGAA 1 cut(s) 1531
OliI CACNNNNGTG 2 cut(s) 194, 1457
PaqCI CACCTGC 1 cut(s) 2494
PceI AGGCCT 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 578
PdmI GAANNNNTTC 3 cut(s) 453, 2099, 3051
PfeI GAWTC 7 cut(s) 310, 395, 593, 1180, 2226, 3037, 3082
PflFI GACNNNGTC 2 cut(s) 491, 2845
PinAI ACCGGT 1 cut(s) 3076
PkrI GCNGC 9 cut(s) 108, 165, 217, 961, 1354, 1432, 2416, 2451, 2758
PleI GAGTC 8 cut(s) 265, 274, 1412, 1442, 1686, 1788, 2636, 2970
PpsI GAGTC 8 cut(s) 265, 274, 1412, 1442, 1686, 1788, 2636, 2970
Psp124BI GAGCTC 2 cut(s) 554, 1709
Psp6I CCWGG 2 cut(s) 457, 2086
PspFI CCCAGC 1 cut(s) 1298
PspGI CCWGG 2 cut(s) 457, 2086
PspN4I GGNNCC 6 cut(s) 291, 429, 1560, 1645, 1809, 2760
PspPI GGNCC 3 cut(s) 1559, 2326, 2480
PstNI CAGNNNCTG 2 cut(s) 959, 2455
PsuI RGATCY 3 cut(s) 1643, 1807, 2008
PsyI GACNNNGTC 2 cut(s) 491, 2845
PvuII CAGCTG 3 cut(s) 1964, 2315, 2513
RsaI GTAC 4 cut(s) 1078, 2302, 2366, 2645
RsaNI GTAC 4 cut(s) 1077, 2301, 2365, 2644
RseI CAYNNNNRTG 6 cut(s) 28, 194, 252, 698, 1457, 2345
SacI GAGCTC 2 cut(s) 554, 1709
SapI GCTCTTC 1 cut(s) 578
SatI GCNGC 9 cut(s) 107, 164, 216, 960, 1353, 1431, 2415, 2450, 2757
Sau3AI GATC 8 cut(s) 40, 1204, 1493, 1522, 1643, 1807, 2008, 2787
Sau96I GGNCC 3 cut(s) 1559, 2326, 2480
ScaI AGTACT 1 cut(s) 2302
SchI GAGTC 8 cut(s) 266, 275, 1412, 1442, 1686, 1789, 2637, 2970
ScrFI CCNGG 3 cut(s) 20, 459, 2088
SduI GDGCHC 4 cut(s) 89, 554, 590, 1709
SfaNI GCATC 8 cut(s) 67, 375, 730, 908, 1240, 1285, 2133, 2185
SfcI CTRYAG 4 cut(s) 807, 1164, 1332, 1959
SfuI TTCGAA 1 cut(s) 1531
SgrAI CRCCGGYG 1 cut(s) 3076
SinI GGWCC 1 cut(s) 2480
SmiMI CAYNNNNRTG 6 cut(s) 28, 194, 252, 698, 1457, 2345
SmlI CTYRAG 4 cut(s) 71, 1358, 1864, 3101
SmoI CTYRAG 4 cut(s) 71, 1358, 1864, 3101
SseBI AGGCCT 1 cut(s) 281
SsiI CCGC 5 cut(s) 106, 163, 901, 2414, 2756
SspMI CTAG 6 cut(s) 209, 314, 1080, 1401, 1514, 2118
SstI GAGCTC 2 cut(s) 554, 1709
StuI AGGCCT 1 cut(s) 281
StyD4I CCNGG 3 cut(s) 18, 457, 2086
StyI CCWWGG 5 cut(s) 1209, 1266, 2232, 2930, 2942
TaaI ACNGT 3 cut(s) 614, 658, 2623
TaiI ACGT 1 cut(s) 1395
TaqI TCGA 8 cut(s) 912, 1174, 1531, 1576, 1830, 1902, 1997, 3090
TatI WGTACW 2 cut(s) 1076, 2300
TauI GCSGC 4 cut(s) 109, 166, 2417, 2759
TfiI GAWTC 7 cut(s) 310, 395, 593, 1180, 2226, 3037, 3082
TscAI CASTG 5 cut(s) 50, 595, 619, 700, 1464
TseFI GTSAC 6 cut(s) 614, 1132, 1415, 1835, 2807, 2847
TseI GCWGC 5 cut(s) 215, 959, 1352, 1430, 2449
Tsp45I GTSAC 6 cut(s) 614, 1132, 1415, 1835, 2807, 2847
TspGWI ACGGA 1 cut(s) 2573
TspRI CASTG 5 cut(s) 50, 595, 619, 700, 1464
Tth111I GACNNNGTC 2 cut(s) 491, 2845
Vha464I CTTAAG 3 cut(s) 1358, 1864, 3101
VpaK11BI GGWCC 1 cut(s) 2480
XapI RAATTY 5 cut(s) 1139, 1410, 2464, 2566, 2746
XceI RCATGY 1 cut(s) 1628
XcmI CCANNNNNNNNNTGG 1 cut(s) 21
XmiI GTMKAC 2 cut(s) 1958, 3121
XmnI GAANNNNTTC 3 cut(s) 453, 2099, 3051
XspI CTAG 6 cut(s) 209, 314, 1080, 1401, 1514, 2118
ZraI GACGTC 1 cut(s) 1393
ZrmI AGTACT 1 cut(s) 2302
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.