MD01G1114300.v1.1

Belongs to the adenylate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012875
Physical Location & Seq
Forward (+)
22880410 .. 22882901
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1114300.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCGGTTCTGAATCGCGTTCTGAAATTACGACGACCGGGAAGCTACTTCTCACTCCGGAGCTTCCACTCCTCGCCTTTTCTAAATTCCGAAACTGACATCAAAGCGTCTGTCTCCGTCATCCCAAAGCCTCTTCCCCTGCATCGCGACGTCAAGGACAGGAATGTCCAGTGGGTCTTCCTCGGCTGCCCCGGCGTCGGCAAAGGCACCTACGCCTCCCGCCTCTGCCACCTCCTCGGCGTCCCCCACATCGCCACCGGCGATCTCGTCCGCGACGAGTTGTCGTCCTCCGGCCCCTTCGCTCTTCAGCTTGCGGAGGTTGTGAATCAGGGGAAATTGGTTTCGGATGAGATTATAATAAACTTGTTGTCCAAGCGTCTTGAGGCTATTGCAGCTAAGGGTGAAACTGGCTTCATCCTTGACGGCTTCCCTCGGACGATTCGACAGGCGGAAATTTTAGAGGGAGTAACAGACATTGACTTGGTGATCAACCTAAAGCTTCGTGAAGAGGCTTTGCTTGCAAAGTGCCTTGGAAGAAGGATTTGTAGTGATTGTGGAGGAAATTATAATGTTGCCTCCGTTGACATTAAGGCTGAGGACGGGAACCCTGGCATGTACATGGCTCCACTTCTTCCCCCTCCAAATTGTGCATCTAAGCTTATCACGCGATCCGATGACACTGAAGAAGTTGTTAAGGAGCGCCTCCGTATTTACAATGATATGAGTCAACCCGTGGAGGAGTTCTACCGAAGTCGAGGAAAATTGTTAGAGTTTGATCTTCCTGGAGGGATTCCTGAATCCTGGCCAAAGCTACTTCAAGCTTTGAATCTGGAAGACCATGAAGACAATCGGTCTGCGGCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 ko01110 ko01130 map00230 map00730 map01100 map01110 map01130
Pfam Domains
Protein Families
ADK

Protein Analysis

289

Amino Acids

31.77

Weight (kDa)

6.02

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADK PF00406 61 - 253 6.1e-38 Adenylate kinase
AAA_17 PF13207 61 - 180 2.4e-20 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39270
fragaria_vesca FvH4_7g20530
malus_domestica MD01G1114300.v1.1 MD07G1182200.v1.1
prunus_persica Prupe.2G220900_v2.0.a1
pyrus_communis pycom01g14330 pycom07g17490
rosa_chinensis RchiOBHm_Chr1g0364421
rosa_laevigata RLG00000027481
rosa_multiflora Rmu_sc0001342.1_g000005
rosa_roxburghii Rroxscaffold_4G00292290
rosa_rugosa Rorug01G0316000 Rorug01G0316000
rosa_samantha Rh1AG324300 Rh1BG287000 Rh1CG303600 Rh1DG318500
rosa_wichuraiana Rw1G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 356, 567
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 153
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 4 cut(s) 18, 147, 273, 667
AccIII TCCGGA 1 cut(s) 57
AciI CCGC 6 cut(s) 5, 220, 271, 314, 449, 857
AclWI GGATC 1 cut(s) 663
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 2 cut(s) 85, 453
AcuI CTGAAG 2 cut(s) 290, 702
AcyI GRCGYC 3 cut(s) 150, 195, 240
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 1 cut(s) 197
AgsI TTSAA 2 cut(s) 818, 826
AhdI GACNNNNNGTC 2 cut(s) 280, 850
AjnI CCWGG 3 cut(s) 607, 781, 800
AluBI AGCT 8 cut(s) 45, 63, 310, 395, 499, 658, 811, 821
AluI AGCT 8 cut(s) 45, 63, 310, 395, 499, 658, 811, 821
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 663
Aor13HI TCCGGA 1 cut(s) 57
AoxI GGCC 2 cut(s) 292, 803
ApeKI GCWGC 3 cut(s) 186, 392, 860
ApoI RAATTY 2 cut(s) 85, 453
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AspLEI GCGC 1 cut(s) 702
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 2 cut(s) 39, 192
AsuHPI GGTGA 2 cut(s) 413, 496
BalI TGGCCA 1 cut(s) 805
BanI GGYRCC 1 cut(s) 206
BbsI GAAGAC 3 cut(s) 169, 840, 849
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 173, 404
BceAI ACGGC 1 cut(s) 439
BciT130I CCWGG 3 cut(s) 609, 783, 802
BclI TGATCA 1 cut(s) 486
BcnI CCSGG 2 cut(s) 39, 192
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 703
BisI GCNGC 4 cut(s) 187, 393, 858, 861
BlsI GCNGC 4 cut(s) 188, 394, 859, 862
Bme1390I CCNGG 5 cut(s) 39, 192, 609, 783, 802
BmeRI GACNNNNNGTC 2 cut(s) 280, 850
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 4 cut(s) 208, 295, 605, 624
BmrFI CCNGG 5 cut(s) 39, 192, 609, 783, 802
BmsI GCATC 2 cut(s) 151, 659
BpiI GAAGAC 3 cut(s) 169, 840, 849
BpmI CTGGAG 1 cut(s) 804
Bpu10I CCTNAGC 2 cut(s) 396, 594
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 2 cut(s) 39, 192
BsaHI GRCGYC 3 cut(s) 150, 195, 240
BsaJI CCNNGG 7 cut(s) 181, 190, 235, 431, 529, 607, 732
BsaWI WCCGGW 1 cut(s) 57
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 169, 412
BseAI TCCGGA 1 cut(s) 57
BseBI CCWGG 3 cut(s) 609, 783, 802
BseDI CCNNGG 7 cut(s) 181, 190, 235, 431, 529, 607, 732
BseGI GGATG 3 cut(s) 120, 352, 414
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 2 cut(s) 169, 412
BseRI GAGGAG 3 cut(s) 61, 224, 752
BseXI GCAGC 2 cut(s) 173, 404
Bsh1236I CGCG 4 cut(s) 18, 147, 273, 667
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 294, 805
BshNI GGYRCC 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 38
BsiSI CCGG 5 cut(s) 38, 58, 192, 258, 291
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 2 cut(s) 294, 805
Bsp13I TCCGGA 1 cut(s) 57
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 4 cut(s) 262, 486, 668, 775
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 6 cut(s) 5, 220, 271, 314, 449, 857
BspANI GGCC 2 cut(s) 294, 805
BspCNI CTCAG 1 cut(s) 586
BspEI TCCGGA 1 cut(s) 57
BspFNI CGCG 4 cut(s) 18, 147, 273, 667
BspLI GGNNCC 4 cut(s) 208, 295, 605, 624
BspPI GGATC 1 cut(s) 663
BspQI GCTCTTC 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 2 cut(s) 169, 412
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 7 cut(s) 181, 190, 235, 431, 529, 607, 732
BssMI GATC 4 cut(s) 262, 486, 668, 775
BssNI GRCGYC 3 cut(s) 150, 195, 240
BssT1I CCWWGG 1 cut(s) 529
Bst2UI CCWGG 3 cut(s) 609, 783, 802
Bst6I CTCTTC 3 cut(s) 138, 309, 501
BstACI GRCGYC 3 cut(s) 150, 195, 240
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 2 cut(s) 312, 519
BstDEI CTNAG 3 cut(s) 396, 594, 654
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 3 cut(s) 120, 352, 414
BstFNI CGCG 4 cut(s) 18, 147, 273, 667
BstH2I RGCGCY 1 cut(s) 703
BstHHI GCGC 1 cut(s) 702
BstKTI GATC 4 cut(s) 265, 489, 671, 778
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 4 cut(s) 262, 486, 668, 775
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 4 cut(s) 192, 392, 518, 664
BstNI CCWGG 3 cut(s) 609, 783, 802
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 5 cut(s) 37, 190, 607, 781, 800
BstUI CGCG 4 cut(s) 18, 147, 273, 667
BstV1I GCAGC 2 cut(s) 173, 404
BstV2I GAAGAC 3 cut(s) 169, 840, 849
BsuRI GGCC 2 cut(s) 294, 805
BtgI CCRYGG 1 cut(s) 732
BtgZI GCGATG 2 cut(s) 128, 235
BtsCI GGATG 3 cut(s) 120, 352, 414
BtsIMutI CAGTG 2 cut(s) 176, 678
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 312, 519
CfoI GCGC 1 cut(s) 702
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 4 cut(s) 96, 184, 229, 365
Csp6I GTAC 1 cut(s) 616
CviAII CATG 4 cut(s) 613, 619, 839, 864
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 3 cut(s) 396, 594, 654
DpnI GATC 4 cut(s) 264, 488, 670, 777
DpnII GATC 4 cut(s) 262, 486, 668, 775
DrdI GACNNNNNNGTC 1 cut(s) 164
DriI GACNNNNNGTC 2 cut(s) 280, 850
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 803
Eam1104I CTCTTC 3 cut(s) 138, 309, 501
Eam1105I GACNNNNNGTC 2 cut(s) 280, 850
EarI CTCTTC 3 cut(s) 138, 309, 501
EciI GGCGGA 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 529
Eco57I CTGAAG 2 cut(s) 290, 702
EcoRII CCWGG 3 cut(s) 607, 781, 800
EcoT14I CCWWGG 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 529
FaeI CATG 4 cut(s) 616, 622, 842, 867
FaiI YATR 7 cut(s) 356, 567, 614, 620, 722, 840, 865
FaqI GGGAC 1 cut(s) 227
FatI CATG 4 cut(s) 612, 618, 838, 863
FauI CCCGC 1 cut(s) 227
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 4 cut(s) 187, 393, 858, 861
FokI GGATG 3 cut(s) 107, 359, 401
Fsp4HI GCNGC 4 cut(s) 187, 393, 858, 861
GlaI GCGC 1 cut(s) 701
GluI GCNGC 4 cut(s) 187, 393, 858, 861
GsuI CTGGAG 1 cut(s) 804
HaeII RGCGCY 1 cut(s) 703
HaeIII GGCC 2 cut(s) 294, 805
HapII CCGG 5 cut(s) 38, 58, 192, 258, 291
HgaI GACGC 4 cut(s) 96, 184, 229, 365
HhaI GCGC 1 cut(s) 702
Hin1I GRCGYC 3 cut(s) 150, 195, 240
Hin1II CATG 4 cut(s) 616, 622, 842, 867
Hin6I GCGC 1 cut(s) 700
HinP1I GCGC 1 cut(s) 700
HincII GTYRAC 2 cut(s) 583, 728
HindII GTYRAC 2 cut(s) 583, 728
HindIII AAGCTT 3 cut(s) 497, 656, 819
HinfI GANTC 7 cut(s) 13, 325, 439, 724, 790, 797, 826
HpaII CCGG 5 cut(s) 38, 58, 192, 258, 291
HphI GGTGA 2 cut(s) 413, 496
Hpy166II GTNNAC 2 cut(s) 583, 728
Hpy188I TCNGA 6 cut(s) 12, 24, 91, 346, 435, 673
Hpy188III TCNNGA 6 cut(s) 58, 146, 380, 503, 794, 830
Hpy8I GTNNAC 2 cut(s) 583, 728
Hpy99I CGWCG 4 cut(s) 36, 152, 200, 278
HpyAV CCTTC 2 cut(s) 307, 531
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 4 cut(s) 142, 392, 521, 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 192, 392, 518, 664
HpyF3I CTNAG 3 cut(s) 396, 594, 654
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 3 cut(s) 150, 195, 240
Hsp92II CATG 4 cut(s) 616, 622, 842, 867
HspAI GCGC 1 cut(s) 700
Kpn2I TCCGGA 1 cut(s) 57
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 4 cut(s) 262, 486, 668, 775
LguI GCTCTTC 1 cut(s) 309
LmnI GCTCC 3 cut(s) 60, 628, 697
Lsp1109I GCAGC 2 cut(s) 173, 404
LweI GCATC 2 cut(s) 151, 659
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 466
MalI GATC 4 cut(s) 264, 488, 670, 777
MboI GATC 4 cut(s) 262, 486, 668, 775
MlsI TGGCCA 1 cut(s) 805
MluCI AATT 7 cut(s) 26, 85, 335, 453, 562, 643, 761
MluNI TGGCCA 1 cut(s) 805
MlyI GAGTC 1 cut(s) 733
Mox20I TGGCCA 1 cut(s) 805
MroI TCCGGA 1 cut(s) 57
MscI TGGCCA 1 cut(s) 805
MseI TTAA 2 cut(s) 588, 693
MslI CAYNNNNRTG 1 cut(s) 617
Msp20I TGGCCA 1 cut(s) 805
MspI CCGG 5 cut(s) 38, 58, 192, 258, 291
MspR9I CCNGG 5 cut(s) 39, 192, 609, 783, 802
MvaI CCWGG 3 cut(s) 609, 783, 802
MvnI CGCG 4 cut(s) 18, 147, 273, 667
MwoI GCNNNNNNNGC 4 cut(s) 192, 392, 518, 664
NciI CCSGG 2 cut(s) 39, 192
NdeII GATC 4 cut(s) 262, 486, 668, 775
NlaIII CATG 4 cut(s) 616, 622, 842, 867
NlaIV GGNNCC 4 cut(s) 208, 295, 605, 624
NmeAIII GCCGAG 2 cut(s) 162, 216
NruI TCGCGA 1 cut(s) 147
NspI RCATGY 1 cut(s) 616
PciSI GCTCTTC 1 cut(s) 309
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 273, 439
PfeI GAWTC 6 cut(s) 13, 325, 439, 790, 797, 826
PfoI TCCNGGA 1 cut(s) 781
PkrI GCNGC 4 cut(s) 188, 394, 859, 862
PleI GAGTC 1 cut(s) 732
PpsI GAGTC 1 cut(s) 732
PsiI TTATAA 2 cut(s) 356, 567
Psp6I CCWGG 3 cut(s) 607, 781, 800
PspGI CCWGG 3 cut(s) 607, 781, 800
PspN4I GGNNCC 4 cut(s) 208, 295, 605, 624
PspPI GGNCC 1 cut(s) 293
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 309
SaqAI TTAA 2 cut(s) 588, 693
SatI GCNGC 4 cut(s) 187, 393, 858, 861
Sau3AI GATC 4 cut(s) 262, 486, 668, 775
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 5 cut(s) 39, 192, 609, 783, 802
SfaNI GCATC 2 cut(s) 151, 659
SgrAI CRCCGGYG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 7 cut(s) 26, 85, 335, 453, 562, 643, 761
SsiI CCGC 6 cut(s) 5, 220, 271, 314, 449, 857
StyD4I CCNGG 5 cut(s) 37, 190, 607, 781, 800
StyI CCWWGG 1 cut(s) 529
TaiI ACGT 1 cut(s) 153
TaqI TCGA 2 cut(s) 442, 754
TaqII GACCGA 1 cut(s) 840
TasI AATT 7 cut(s) 26, 85, 335, 453, 562, 643, 761
TatI WGTACW 1 cut(s) 615
TauI GCSGC 1 cut(s) 860
TfiI GAWTC 6 cut(s) 13, 325, 439, 790, 797, 826
Tru1I TTAA 2 cut(s) 588, 693
Tru9I TTAA 2 cut(s) 588, 693
TscAI CASTG 2 cut(s) 176, 685
TseI GCWGC 3 cut(s) 186, 392, 860
TspDTI ATGAA 2 cut(s) 403, 855
TspGWI ACGGA 3 cut(s) 106, 568, 695
TspRI CASTG 2 cut(s) 176, 685
XapI RAATTY 2 cut(s) 85, 453
XceI RCATGY 1 cut(s) 616
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.