FvH4_7g22070

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016116
Physical Location & Seq
Reverse (-)
17498143 .. 17499864
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22070.t1

Sequence Viewer

Length: 588 bp
ATGCGCTCCTCCGCTCAGCCCCAAAACGGCGGCGAAAACTACCCAATCCACCACCCTAACTTCCACTCCACCGTCTCCCTCCGCAAGCTCCGCCGCTTCAACTACCTCATCCTCCTCTTCCGCATCGCCGCCTTCAGCTCCTCCCTCGCCTCCTTCGTCTTCATGCTCTCCACCTCCCGCTCCTCCTCCGCCCCCTCCTCTCCTCGCTGGTACCACTTCGACGCCTTCAGATTGGTGGTGGGAGCGAACGCGATAGTGGCGGTATACTCCCTCTTCGAAATGGTGGCCTCAGTTTGGGAGATTTCCAGAGGCGCCACGCTCTTCAGCGAGGTGTTACAAGTCTGGTTCGACTTCGGCCATGACCAGGTGTTCGCGTACCTGCTGCTCTCTGCGGACTCGGCGGGAACGGCGCTGGCGAGGGTTTTGAAAGGGATGGACACGTGTACGGAGTGGAGCGCGTTCTGCATAGAGACGGACATTGCGATTTCGTTGGGGTTTGTCGGGTTTTTGTTCCTGGGCTTTTCGTCCGTGCTCTCGGGTTTTCGGGTCGCCTGCTTCGTAATAAAGGGTTCTCGTTTTCATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

196

Amino Acids

21.69

Weight (kDa)

8.93

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 36 - 171 7e-28 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55390
fragaria_vesca FvH4_7g22070
malus_domestica MD01G1125700.v1.1
prunus_persica Prupe.2G232700_v2.0.a1
pyrus_communis pycom01g15150
rosa_chinensis RchiOBHm_Chr1g0366461
rosa_laevigata RLG00000027346
rosa_roxburghii Rroxscaffold_4G00289920 Rroxscaffold_4G00290180
rosa_rugosa Rorug01G0330000
rosa_samantha Rh1AG337900 Rh1BG299400 Rh1CG314500 Rh1DG331200
rosa_wichuraiana Rw1G029920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 387
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 2 cut(s) 210, 311
AccBSI CCGCTC 2 cut(s) 14, 180
AccI GTMKAC 1 cut(s) 264
AccII CGCG 3 cut(s) 251, 374, 458
AcoI YGGCCR 1 cut(s) 355
AcuI CTGAAG 3 cut(s) 118, 211, 307
AcvI CACGTG 1 cut(s) 441
AcyI GRCGYC 2 cut(s) 222, 312
AfaI GTAC 3 cut(s) 212, 377, 445
AfiI CCNNNNNNNGG 3 cut(s) 26, 294, 364
AflIII ACRYGT 2 cut(s) 438, 440
AgsI TTSAA 2 cut(s) 100, 427
AjnI CCWGG 2 cut(s) 363, 513
AluBI AGCT 2 cut(s) 88, 138
AluI AGCT 2 cut(s) 88, 138
Alw21I GWGCWC 1 cut(s) 534
Alw26I GTCTC 2 cut(s) 79, 464
Ama87I CYCGRG 1 cut(s) 535
AoxI GGCC 2 cut(s) 285, 355
ApeKI GCWGC 1 cut(s) 382
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
Asp718I GGTACC 1 cut(s) 210
AspLEI GCGC 4 cut(s) 6, 314, 412, 458
AsuII TTCGAA 1 cut(s) 276
AvaI CYCGRG 1 cut(s) 535
BanI GGYRCC 2 cut(s) 210, 311
BbrPI CACGTG 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 151
Bbv12I GWGCWC 1 cut(s) 534
BbvI GCAGC 1 cut(s) 369
BccI CCATC 1 cut(s) 427
BceAI ACGGC 2 cut(s) 43, 423
BciT130I CCWGG 2 cut(s) 365, 515
BcoDI GTCTC 2 cut(s) 79, 464
BfaI CTAG 1 cut(s) 586
BfoI RGCGCY 2 cut(s) 315, 413
BfuAI ACCTGC 1 cut(s) 387
BisI GCNGC 4 cut(s) 31, 94, 129, 383
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 4 cut(s) 32, 95, 130, 384
Bme1390I CCNGG 2 cut(s) 365, 515
BmeT110I CYCGRG 1 cut(s) 535
BmiI GGNNCC 2 cut(s) 212, 313
BmrFI CCNGG 2 cut(s) 365, 515
BmsI GCATC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 151
Bpu1102I GCTNAGC 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 276
BsaAI YACGTR 1 cut(s) 441
BsaHI GRCGYC 2 cut(s) 222, 312
BsaJI CCNNGG 1 cut(s) 514
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 294, 364
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 2 cut(s) 365, 515
BseDI CCNNGG 1 cut(s) 514
BseGI GGATG 2 cut(s) 108, 438
BseLI CCNNNNNNNGG 3 cut(s) 26, 294, 364
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 2 cut(s) 29, 303
BseRI GAGGAG 6 cut(s) 104, 130, 172, 175, 187, 192
BseXI GCAGC 1 cut(s) 369
Bsh1236I CGCG 3 cut(s) 251, 374, 458
BshFI GGCC 2 cut(s) 287, 357
BshNI GGYRCC 2 cut(s) 210, 311
BsiHKAI GWGCWC 1 cut(s) 534
BsiHKCI CYCGRG 1 cut(s) 535
BslI CCNNNNNNNGG 3 cut(s) 26, 294, 364
BsmAI GTCTC 2 cut(s) 79, 464
BsmBI CGTCTC 2 cut(s) 79, 464
BsnI GGCC 2 cut(s) 287, 357
BsoBI CYCGRG 1 cut(s) 535
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 534
Bsp1720I GCTNAGC 1 cut(s) 15
BspANI GGCC 2 cut(s) 287, 357
BspCNI CTCAG 2 cut(s) 28, 302
BspFNI CGCG 3 cut(s) 251, 374, 458
BspLI GGNNCC 2 cut(s) 212, 313
BspMI ACCTGC 1 cut(s) 387
BspQI GCTCTTC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 276
BspT107I GGYRCC 2 cut(s) 210, 311
BsrBI CCGCTC 2 cut(s) 14, 180
BsrDI GCAATG 1 cut(s) 477
BssECI CCNNGG 1 cut(s) 514
BssNAI GTATAC 1 cut(s) 265
BssNI GRCGYC 2 cut(s) 222, 312
Bst1107I GTATAC 1 cut(s) 265
Bst2UI CCWGG 2 cut(s) 365, 515
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 3 cut(s) 122, 278, 326
BstACI GRCGYC 2 cut(s) 222, 312
BstBAI YACGTR 1 cut(s) 441
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 3 cut(s) 86, 414, 553
BstDEI CTNAG 2 cut(s) 15, 289
BstF5I GGATG 2 cut(s) 108, 438
BstFNI CGCG 3 cut(s) 251, 374, 458
BstH2I RGCGCY 2 cut(s) 315, 413
BstHHI GCGC 4 cut(s) 6, 314, 412, 458
BstMAI GTCTC 2 cut(s) 79, 464
BstMWI GCNNNNNNNGC 5 cut(s) 90, 257, 398, 407, 462
BstNI CCWGG 2 cut(s) 365, 515
BstSCI CCNGG 2 cut(s) 363, 513
BstUI CGCG 3 cut(s) 251, 374, 458
BstV1I GCAGC 1 cut(s) 369
BstV2I GAAGAC 1 cut(s) 151
BstZ17I GTATAC 1 cut(s) 265
BsuRI GGCC 2 cut(s) 287, 357
BtgZI GCGATG 1 cut(s) 109
BtsCI GGATG 2 cut(s) 108, 438
BveI ACCTGC 1 cut(s) 387
Cac8I GCNNGC 3 cut(s) 86, 414, 553
CfoI GCGC 4 cut(s) 6, 314, 412, 458
CseI GACGC 1 cut(s) 230
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 3 cut(s) 211, 376, 444
CviAII CATG 2 cut(s) 163, 359
CviJI RGCY 6 cut(s) 19, 88, 138, 287, 357, 519
CviKI_1 RGCY 6 cut(s) 19, 88, 138, 287, 357, 519
CviQI GTAC 3 cut(s) 211, 376, 444
DdeI CTNAG 2 cut(s) 15, 289
DinI GGCGCC 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 355
Eam1104I CTCTTC 3 cut(s) 122, 278, 326
EarI CTCTTC 3 cut(s) 122, 278, 326
EciI GGCGGA 2 cut(s) 80, 178
Eco57I CTGAAG 3 cut(s) 118, 211, 307
Eco72I CACGTG 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 535
EcoRII CCWGG 2 cut(s) 363, 513
EgeI GGCGCC 1 cut(s) 313
EheI GGCGCC 1 cut(s) 313
Esp3I CGTCTC 2 cut(s) 79, 464
FaeI CATG 2 cut(s) 166, 362
FaiI YATR 4 cut(s) 164, 265, 360, 467
FatI CATG 2 cut(s) 162, 358
FauI CCCGC 2 cut(s) 185, 394
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 4 cut(s) 31, 94, 129, 383
FokI GGATG 2 cut(s) 95, 445
Fsp4HI GCNGC 4 cut(s) 31, 94, 129, 383
FspBI CTAG 1 cut(s) 586
GlaI GCGC 4 cut(s) 5, 313, 411, 457
GluI GCNGC 4 cut(s) 31, 94, 129, 383
HaeII RGCGCY 2 cut(s) 315, 413
HaeIII GGCC 2 cut(s) 287, 357
HgaI GACGC 1 cut(s) 230
HhaI GCGC 4 cut(s) 6, 314, 412, 458
Hin1I GRCGYC 2 cut(s) 222, 312
Hin1II CATG 2 cut(s) 166, 362
Hin6I GCGC 4 cut(s) 4, 312, 410, 456
HinP1I GCGC 4 cut(s) 4, 312, 410, 456
HinfI GANTC 1 cut(s) 395
Hpy166II GTNNAC 2 cut(s) 265, 444
Hpy188I TCNGA 1 cut(s) 230
Hpy188III TCNNGA 1 cut(s) 306
Hpy8I GTNNAC 2 cut(s) 265, 444
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 3 cut(s) 142, 163, 235
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 1 cut(s) 465
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 257, 398, 407, 462
HpyF3I CTNAG 2 cut(s) 15, 289
HpySE526I ACGT 1 cut(s) 440
Hsp92I GRCGYC 2 cut(s) 222, 312
Hsp92II CATG 2 cut(s) 166, 362
HspAI GCGC 4 cut(s) 4, 312, 410, 456
KasI GGCGCC 1 cut(s) 311
KpnI GGTACC 1 cut(s) 214
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 6 cut(s) 11, 93, 143, 185, 242, 453
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 1 cut(s) 132
MabI ACCWGGT 1 cut(s) 363
MaeI CTAG 1 cut(s) 586
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 1 cut(s) 333
MbiI CCGCTC 2 cut(s) 14, 180
MboII GAAGA 4 cut(s) 109, 151, 265, 313
MhlI GDGCHC 1 cut(s) 534
Mly113I GGCGCC 1 cut(s) 312
MlyI GAGTC 1 cut(s) 389
MspR9I CCNGG 2 cut(s) 365, 515
MvaI CCWGG 2 cut(s) 365, 515
MvnI CGCG 3 cut(s) 251, 374, 458
MwoI GCNNNNNNNGC 5 cut(s) 90, 257, 398, 407, 462
NarI GGCGCC 1 cut(s) 312
NlaIII CATG 2 cut(s) 166, 362
NlaIV GGNNCC 2 cut(s) 212, 313
NmeAIII GCCGAG 1 cut(s) 377
NspV TTCGAA 1 cut(s) 276
PciSI GCTCTTC 1 cut(s) 326
PcsI WCGNNNNNNNCGW 4 cut(s) 153, 413, 479, 555
PkrI GCNGC 4 cut(s) 32, 95, 130, 384
PleI GAGTC 1 cut(s) 389
PluTI GGCGCC 1 cut(s) 315
PmaCI CACGTG 1 cut(s) 441
PmlI CACGTG 1 cut(s) 441
PpsI GAGTC 1 cut(s) 389
Ppu21I YACGTR 1 cut(s) 441
Psp6I CCWGG 2 cut(s) 363, 513
PspCI CACGTG 1 cut(s) 441
PspGI CCWGG 2 cut(s) 363, 513
PspN4I GGNNCC 2 cut(s) 212, 313
RsaI GTAC 3 cut(s) 212, 377, 445
RsaNI GTAC 3 cut(s) 211, 376, 444
SapI GCTCTTC 1 cut(s) 326
SatI GCNGC 4 cut(s) 31, 94, 129, 383
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 365, 515
SduI GDGCHC 1 cut(s) 534
SetI ASST 8 cut(s) 90, 108, 140, 176, 333, 369, 381, 443
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 1 cut(s) 132
SfoI GGCGCC 1 cut(s) 313
SfuI TTCGAA 1 cut(s) 276
SspDI GGCGCC 1 cut(s) 311
SspMI CTAG 1 cut(s) 586
StyD4I CCNGG 2 cut(s) 363, 513
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 443
TaqI TCGA 3 cut(s) 219, 276, 348
TauI GCSGC 3 cut(s) 33, 96, 131
TseI GCWGC 1 cut(s) 382
TspDTI ATGAA 2 cut(s) 151, 569
TspGWI ACGGA 3 cut(s) 461, 488, 517
XmiI GTMKAC 1 cut(s) 264
XspI CTAG 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.