MD01G1125700.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016116
Physical Location & Seq
Reverse (-)
23783550 .. 23785304
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1125700.v1.1.491

Sequence Viewer

Length: 564 bp
ATGCGCTCTCCTCAGCCTCACCGCAACGGCGGCGACAATCCCTTCCACTCCACCGTCTCGCTCCGGAAGCTGAGGCGCTTCAACTACCTCATCCTCGTCTTCCGATTCGCCTCCTTCAGCTCCTCCCTCGCTTCCTTCGTCTTCATGCTCTCCACCTCTCGTGCCGCCGCCAACTCCCCCCGCTGGTACCACTTCGACGCCTTCAGATTCGTAGTGGGAGCGAATGCAATAGTGGCGCTCTACTCTCTCTTCGAAATGGTGGCTTCCGTCTGGGAAATTTCCAGAGAATCCACTCTCTTCGCCGAGGTCGTCCAGCTCTGGTTCGACTTCGGCCACGACCAGGCGTTCGCGTACCTGTTGCTGTCTGCAGACTCGGCGGGAACGGCGTTGGCGAGGGTTTTAAAAGGGATGGACACGTGTAAGGCGTGGAACGCGTTCTGCATACAGGTGGACATCGCAATCGCTTTGGGCTTTGTGGGGTTCCTGTTCCTGGGCTTCTCATCTCTCCTCTCGGGCTTTCGGATCGCCTGCTTCATCATCAAGGGATCTCGTTTTCATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

188

Amino Acids

20.98

Weight (kDa)

9.65

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 30 - 163 7.9e-28 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G55390
fragaria_vesca FvH4_7g22070
malus_domestica MD01G1125700.v1.1
prunus_persica Prupe.2G232700_v2.0.a1
pyrus_communis pycom01g15150
rosa_chinensis RchiOBHm_Chr1g0366461
rosa_laevigata RLG00000027346
rosa_roxburghii Rroxscaffold_4G00289920 Rroxscaffold_4G00290180
rosa_rugosa Rorug01G0330000
rosa_samantha Rh1AG337900 Rh1BG299400 Rh1CG314500 Rh1DG331200
rosa_wichuraiana Rw1G029920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 186
AccII CGCG 2 cut(s) 350, 434
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 6 cut(s) 22, 30, 165, 168, 181, 377
AclWI GGATC 2 cut(s) 530, 553
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 2 cut(s) 100, 187
AcvI CACGTG 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 188, 353
AfiI CCNNNNNNNGG 3 cut(s) 183, 340, 490
AflIII ACRYGT 3 cut(s) 414, 416, 432
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 2 cut(s) 339, 489
AluBI AGCT 3 cut(s) 70, 120, 316
AluI AGCT 3 cut(s) 70, 120, 316
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 2 cut(s) 530, 553
Ama87I CYCGRG 1 cut(s) 511
Aor13HI TCCGGA 1 cut(s) 63
AoxI GGCC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 276
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
Asp700I GAANNNNTTC 1 cut(s) 434
Asp718I GGTACC 1 cut(s) 186
AspLEI GCGC 3 cut(s) 6, 78, 238
AsuHPI GGTGA 1 cut(s) 11
AsuII TTCGAA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 511
BanI GGYRCC 1 cut(s) 186
BauI CACGAG 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 417
BbsI GAAGAC 2 cut(s) 91, 133
BbvCI CCTCAGC 2 cut(s) 12, 71
BccI CCATC 1 cut(s) 403
BceAI ACGGC 2 cut(s) 43, 399
BciT130I CCWGG 2 cut(s) 341, 491
BcoDI GTCTC 1 cut(s) 61
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 2 cut(s) 79, 239
BisI GCNGC 3 cut(s) 31, 165, 168
BlsI GCNGC 3 cut(s) 32, 166, 169
Bme1390I CCNGG 2 cut(s) 341, 491
BmeT110I CYCGRG 1 cut(s) 511
BmiI GGNNCC 2 cut(s) 188, 482
BmrFI CCNGG 2 cut(s) 341, 491
BpiI GAAGAC 2 cut(s) 91, 133
Bpu10I CCTNAGC 2 cut(s) 12, 71
Bpu14I TTCGAA 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 303, 490
BsaWI WCCGGW 1 cut(s) 63
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 340, 490
BseAI TCCGGA 1 cut(s) 63
BseBI CCWGG 2 cut(s) 341, 491
BseDI CCNNGG 2 cut(s) 303, 490
BseGI GGATG 2 cut(s) 90, 414
BseLI CCNNNNNNNGG 3 cut(s) 183, 340, 490
BseMII CTCAG 2 cut(s) 26, 62
BseRI GAGGAG 2 cut(s) 112, 497
Bsh1236I CGCG 2 cut(s) 350, 434
BshFI GGCC 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 1 cut(s) 64
BslI CCNNNNNNNGG 3 cut(s) 183, 340, 490
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 252
Bsp13I TCCGGA 1 cut(s) 63
Bsp143I GATC 2 cut(s) 522, 545
BspACI CCGC 6 cut(s) 22, 30, 165, 168, 181, 377
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 2 cut(s) 25, 63
BspEI TCCGGA 1 cut(s) 63
BspFNI CGCG 2 cut(s) 350, 434
BspLI GGNNCC 2 cut(s) 188, 482
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 2 cut(s) 530, 553
BspT104I TTCGAA 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 186
BssECI CCNNGG 2 cut(s) 303, 490
BssMI GATC 2 cut(s) 522, 545
BssNI GRCGYC 1 cut(s) 198
BssSI CACGAG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 2 cut(s) 341, 491
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 2 cut(s) 254, 302
BstACI GRCGYC 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 417
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 2 cut(s) 12, 71
BstF5I GGATG 2 cut(s) 90, 414
BstFNI CGCG 2 cut(s) 350, 434
BstH2I RGCGCY 2 cut(s) 79, 239
BstHHI GCGC 3 cut(s) 6, 78, 238
BstKTI GATC 2 cut(s) 525, 548
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 2 cut(s) 522, 545
BstMWI GCNNNNNNNGC 6 cut(s) 30, 67, 233, 374, 383, 431
BstNI CCWGG 2 cut(s) 341, 491
BstSCI CCNGG 2 cut(s) 339, 489
BstSFI CTRYAG 1 cut(s) 366
BstUI CGCG 2 cut(s) 350, 434
BstV2I GAAGAC 2 cut(s) 91, 133
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
BsuRI GGCC 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 439
BtsCI GGATG 2 cut(s) 90, 414
Cac8I GCNNGC 1 cut(s) 529
CfoI GCGC 3 cut(s) 6, 78, 238
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 187, 352
CviAII CATG 1 cut(s) 145
CviJI RGCY 9 cut(s) 16, 70, 120, 263, 316, 333, 471, 495, 516
CviKI_1 RGCY 9 cut(s) 16, 70, 120, 263, 316, 333, 471, 495, 516
CviQI GTAC 2 cut(s) 187, 352
DdeI CTNAG 2 cut(s) 12, 71
DpnI GATC 2 cut(s) 524, 547
DpnII GATC 2 cut(s) 522, 545
DraI TTTAAA 1 cut(s) 402
EaeI YGGCCR 1 cut(s) 331
Eam1104I CTCTTC 2 cut(s) 254, 302
EarI CTCTTC 2 cut(s) 254, 302
Eco57I CTGAAG 2 cut(s) 100, 187
Eco72I CACGTG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 511
EcoRII CCWGG 2 cut(s) 339, 489
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 1 cut(s) 148
FaiI YATR 2 cut(s) 146, 443
FatI CATG 1 cut(s) 144
FauI CCCGC 2 cut(s) 188, 370
Fnu4HI GCNGC 3 cut(s) 31, 165, 168
FokI GGATG 2 cut(s) 77, 421
Fsp4HI GCNGC 3 cut(s) 31, 165, 168
GlaI GCGC 3 cut(s) 5, 77, 237
GluI GCNGC 3 cut(s) 31, 165, 168
HaeII RGCGCY 2 cut(s) 79, 239
HaeIII GGCC 1 cut(s) 333
HapII CCGG 1 cut(s) 64
HgaI GACGC 1 cut(s) 206
HhaI GCGC 3 cut(s) 6, 78, 238
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 3 cut(s) 4, 76, 236
HinP1I GCGC 3 cut(s) 4, 76, 236
HinfI GANTC 4 cut(s) 105, 207, 287, 371
HpaII CCGG 1 cut(s) 64
HphI GGTGA 1 cut(s) 11
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 3 cut(s) 104, 206, 522
Hpy188III TCNNGA 2 cut(s) 64, 282
Hpy8I GTNNAC 1 cut(s) 451
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 4 cut(s) 52, 124, 145, 211
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 3 cut(s) 227, 368, 441
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 67, 233, 374, 383, 431
HpyF3I CTNAG 2 cut(s) 12, 71
HpySE526I ACGT 1 cut(s) 416
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 3 cut(s) 4, 76, 236
Kpn2I TCCGGA 1 cut(s) 63
KpnI GGTACC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 522, 545
LmnI GCTCC 3 cut(s) 66, 125, 218
MaeII ACGT 1 cut(s) 416
MalI GATC 2 cut(s) 524, 547
MboI GATC 2 cut(s) 522, 545
MboII GAAGA 4 cut(s) 91, 133, 241, 289
MflI RGATCY 1 cut(s) 545
MluCI AATT 1 cut(s) 276
MluI ACGCGT 1 cut(s) 432
MlyI GAGTC 1 cut(s) 365
MroI TCCGGA 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 434
MseI TTAA 1 cut(s) 401
MslI CAYNNNNRTG 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 2 cut(s) 341, 491
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 2 cut(s) 341, 491
MvnI CGCG 2 cut(s) 350, 434
MwoI GCNNNNNNNGC 6 cut(s) 30, 67, 233, 374, 383, 431
NdeII GATC 2 cut(s) 522, 545
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 188, 482
NmeAIII GCCGAG 2 cut(s) 328, 353
NspV TTCGAA 1 cut(s) 252
PcsI WCGNNNNNNNCGW 4 cut(s) 135, 306, 389, 422
PctI GAATGC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 3 cut(s) 105, 207, 287
PkrI GCNGC 3 cut(s) 32, 166, 169
PleI GAGTC 1 cut(s) 365
PmaCI CACGTG 1 cut(s) 417
PmlI CACGTG 1 cut(s) 417
PpsI GAGTC 1 cut(s) 365
Ppu21I YACGTR 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 339, 489
PspCI CACGTG 1 cut(s) 417
PspGI CCWGG 2 cut(s) 339, 489
PspN4I GGNNCC 2 cut(s) 188, 482
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 545
RsaI GTAC 2 cut(s) 188, 353
RsaNI GTAC 2 cut(s) 187, 352
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 3 cut(s) 31, 165, 168
Sau3AI GATC 2 cut(s) 522, 545
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 2 cut(s) 341, 491
SetI ASST 9 cut(s) 72, 90, 122, 158, 309, 318, 357, 419, 450
SfcI CTRYAG 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 1 cut(s) 276
SsiI CCGC 6 cut(s) 22, 30, 165, 168, 181, 377
StyD4I CCNGG 2 cut(s) 339, 489
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 419
TaqI TCGA 3 cut(s) 195, 252, 324
TasI AATT 1 cut(s) 276
TauI GCSGC 3 cut(s) 33, 167, 170
TfiI GAWTC 3 cut(s) 105, 207, 287
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TspDTI ATGAA 3 cut(s) 133, 523, 545
TspGWI ACGGA 1 cut(s) 256
XapI RAATTY 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.