MD00G1015600.v1.1

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0022414
Physical Location & Seq
Reverse (-)
2245255 .. 2247379
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1015600.v1.1.491

Sequence Viewer

Length: 594 bp
ATGAAAGAATACCTCACACCAATGGGGCCAACTCCTCTTCACGTGAACCCAATCTTTGAGACAGGCCTGATTGAGCCAAGATTCTCAGAATGGTTGGTGTTTGAGGGCATCAGTGTTGACGAGAGTGGGAAGCAGCATTACCTAGATGCAACTGTGGCTTACAAGCCAGCAGTACTCAATGCAATTGACTACCTCTCTAAATTTGGATACTCCAAAGAACAGGTTTTCGACCTCTCTCTTTATCACATAGACGATTCCCCACAAACGCACAATCACAAGCATACACCGAGACACCGAAAGACATGCCTACCTAAATATATTATTGTAGTCTTCCATACGGATATGGCATATGTCATATATCAGAGCTATCTTCTGTTGTCATGCTGCCCGTGTGAGGGAAGAATTTCAGGAATAGTTGACTCTCCCAATGCTGTTGCAACTATAGCAATCCCAACATCTATCTTTGACCAGGATATTCGCCCAAAAGAAAACAAGGTTCCTGTTGGTCCCTGGATAGTGAGGAAGCCAGATGTCCTAAAATGTACTTATGATGGAAATTTGCCAACGACAAGGAACCCTAGCTCTGCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

198

Amino Acids

22.25

Weight (kDa)

6.43

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 16 - 78 1.2e-22 Acetamidase/Formamidase family
FmdA_AmdA PF03069 109 - 157 1.3e-13 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022414)

Species Orthologous Gene IDs
malus_domestica MD00G1015600.v1.1
rosa_multiflora Rmu_sc0001149.1_g000017
rosa_samantha Rh2BG208000 Rh2CG200800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 200, 402, 556
AcvI CACGTG 1 cut(s) 43
AfaI GTAC 2 cut(s) 174, 544
AfiI CCNNNNNNNGG 2 cut(s) 394, 395
AjnI CCWGG 2 cut(s) 468, 509
AluBI AGCT 2 cut(s) 366, 582
AluI AGCT 2 cut(s) 366, 582
Alw26I GTCTC 2 cut(s) 53, 283
AoxI GGCC 2 cut(s) 26, 64
ApeKI GCWGC 2 cut(s) 133, 384
ApoI RAATTY 3 cut(s) 200, 402, 556
Asp700I GAANNNNTTC 1 cut(s) 403
AspS9I GGNCC 2 cut(s) 26, 506
AvaII GGWCC 1 cut(s) 506
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BbrPI CACGTG 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 322
BbvI GCAGC 2 cut(s) 145, 371
BccI CCATC 1 cut(s) 545
BcgI CGANNNNNNTGC 2 cut(s) 285, 319
BciT130I CCWGG 2 cut(s) 470, 511
BciVI GTATCC 1 cut(s) 200
BcoDI GTCTC 2 cut(s) 53, 283
BfaI CTAG 2 cut(s) 143, 579
BfmI CTRYAG 1 cut(s) 441
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 2 cut(s) 134, 385
BlsI GCNGC 2 cut(s) 135, 386
BmcAI AGTACT 1 cut(s) 174
Bme1390I CCNGG 2 cut(s) 470, 511
Bme18I GGWCC 1 cut(s) 506
BmgT120I GGNCC 2 cut(s) 26, 506
BmiI GGNNCC 4 cut(s) 27, 498, 508, 575
BmrFI CCNGG 2 cut(s) 470, 511
BmsI GCATC 2 cut(s) 117, 136
BpiI GAAGAC 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 509
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 395
BseBI CCWGG 2 cut(s) 470, 511
BseDI CCNNGG 1 cut(s) 509
BseLI CCNNNNNNNGG 2 cut(s) 394, 395
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 2 cut(s) 145, 371
BshFI GGCC 2 cut(s) 28, 66
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 2 cut(s) 394, 395
BsmAI GTCTC 2 cut(s) 53, 283
BsmFI GGGAC 1 cut(s) 492
BsnI GGCC 2 cut(s) 28, 66
BspANI GGCC 2 cut(s) 28, 66
BspCNI CTCAG 1 cut(s) 98
BspLI GGNNCC 4 cut(s) 27, 498, 508, 575
BssECI CCNNGG 1 cut(s) 509
Bst2UI CCWGG 2 cut(s) 470, 511
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 42
BstBAI YACGTR 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 1 cut(s) 85
BstMAI GTCTC 2 cut(s) 53, 283
BstMWI GCNNNNNNNGC 2 cut(s) 155, 443
BstNI CCWGG 2 cut(s) 470, 511
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 2 cut(s) 468, 509
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 145, 371
BstV2I GAAGAC 1 cut(s) 322
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 2 cut(s) 28, 66
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 26, 506
Csp6I GTAC 2 cut(s) 173, 543
CviAII CATG 2 cut(s) 303, 381
CviJI RGCY 8 cut(s) 28, 66, 76, 158, 166, 366, 526, 582
CviKI_1 RGCY 8 cut(s) 28, 66, 76, 158, 166, 366, 526, 582
CviQI GTAC 2 cut(s) 173, 543
DdeI CTNAG 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 506
Eco72I CACGTG 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 468, 509
FaeI CATG 2 cut(s) 306, 384
FaqI GGGAC 1 cut(s) 492
FatI CATG 2 cut(s) 302, 380
FauNDI CATATG 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 134, 385
Fsp4HI GCNGC 2 cut(s) 134, 385
FspBI CTAG 2 cut(s) 143, 579
GluI GCNGC 2 cut(s) 134, 385
HaeIII GGCC 2 cut(s) 28, 66
Hin1II CATG 2 cut(s) 306, 384
HincII GTYRAC 2 cut(s) 118, 418
HindII GTYRAC 2 cut(s) 118, 418
HinfI GANTC 3 cut(s) 81, 254, 419
Hpy166II GTNNAC 3 cut(s) 46, 118, 418
Hpy188I TCNGA 2 cut(s) 88, 363
Hpy188III TCNNGA 1 cut(s) 408
Hpy8I GTNNAC 3 cut(s) 46, 118, 418
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 149, 182, 437, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 443
HpyF3I CTNAG 1 cut(s) 85
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 2 cut(s) 306, 384
Lsp1109I GCAGC 2 cut(s) 145, 371
LweI GCATC 2 cut(s) 117, 136
MaeI CTAG 2 cut(s) 143, 579
MaeII ACGT 1 cut(s) 42
MboII GAAGA 4 cut(s) 29, 322, 362, 411
MfeI CAATTG 1 cut(s) 183
MluCI AATT 4 cut(s) 183, 200, 402, 556
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 7 cut(s) 23, 45, 97, 203, 242, 388, 513
MroXI GAANNNNTTC 1 cut(s) 403
MslI CAYNNNNRTG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 470, 511
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 2 cut(s) 470, 511
MwoI GCNNNNNNNGC 2 cut(s) 155, 443
NdeI CATATG 1 cut(s) 349
NlaIII CATG 2 cut(s) 306, 384
NlaIV GGNNCC 4 cut(s) 27, 498, 508, 575
NspI RCATGY 1 cut(s) 306
PceI AGGCCT 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 2 cut(s) 81, 254
PkrI GCNGC 2 cut(s) 135, 386
PleI GAGTC 1 cut(s) 413
PmaCI CACGTG 1 cut(s) 43
PmlI CACGTG 1 cut(s) 43
PpsI GAGTC 1 cut(s) 413
Ppu21I YACGTR 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 468, 509
PspCI CACGTG 1 cut(s) 43
PspGI CCWGG 2 cut(s) 468, 509
PspN4I GGNNCC 4 cut(s) 27, 498, 508, 575
PspPI GGNCC 2 cut(s) 26, 506
RsaI GTAC 2 cut(s) 174, 544
RsaNI GTAC 2 cut(s) 173, 543
RseI CAYNNNNRTG 1 cut(s) 20
SatI GCNGC 2 cut(s) 134, 385
Sau96I GGNCC 2 cut(s) 26, 506
ScaI AGTACT 1 cut(s) 174
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 2 cut(s) 470, 511
SfaNI GCATC 2 cut(s) 117, 136
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 1 cut(s) 506
SmiMI CAYNNNNRTG 1 cut(s) 20
Sse9I AATT 4 cut(s) 183, 200, 402, 556
SseBI AGGCCT 1 cut(s) 66
SspMI CTAG 2 cut(s) 143, 579
StuI AGGCCT 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 468, 509
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 1 cut(s) 45
TaqI TCGA 1 cut(s) 228
TasI AATT 4 cut(s) 183, 200, 402, 556
TatI WGTACW 2 cut(s) 172, 542
TfiI GAWTC 2 cut(s) 81, 254
TscAI CASTG 1 cut(s) 118
TseI GCWGC 2 cut(s) 133, 384
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 3 cut(s) 200, 402, 556
XceI RCATGY 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 403
XspI CTAG 2 cut(s) 143, 579
ZrmI AGTACT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.