Rh2CG200800

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0022414
Physical Location & Seq
Forward (+)
19293539 .. 19334013
40475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG200800.1

Sequence Viewer

Length: 291 bp
ATGAATGATTTGGTCAGTAAAGCCAGTACATTAATTACATATGCAAACCCGGAGCACGTGTCTCCATGTGAAGGACTACAAGCTTATTTCAATCTCTCCTCTACTACTAAAAGTCCCGTCGAGCCAAGATTCTCGGAATGGTTGGTATTTGAGGGCATCAGTGTTGATGAGAGTGGGAAGCAGCATTACCTAGATGCAACTGTTGCTTACAAGCGTGCAGTACTGAATGCCATCGACTACCTCTCCAAATTTGGATACTCAAAAGAACAGTCTTCTTCGTTGCTGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

96

Amino Acids

10.68

Weight (kDa)

5.21

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 36 - 95 1.2e-21 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022414)

Species Orthologous Gene IDs
malus_domestica MD00G1015600.v1.1
rosa_multiflora Rmu_sc0001149.1_g000017
rosa_samantha Rh2BG208000 Rh2CG200800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 248
AcvI CACGTG 1 cut(s) 58
AfaI GTAC 2 cut(s) 28, 222
AfiI CCNNNNNNNGG 1 cut(s) 71
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 1 cut(s) 91
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 181
ApoI RAATTY 1 cut(s) 248
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AseI ATTAAT 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 50
BarI GAAGNNNNNNTAC 2 cut(s) 170, 202
BbrPI CACGTG 1 cut(s) 58
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 193
BccI CCATC 1 cut(s) 239
BciVI GTATCC 1 cut(s) 248
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 2 cut(s) 191, 289
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 1 cut(s) 182
BlsI GCNGC 1 cut(s) 183
BmcAI AGTACT 1 cut(s) 222
Bme1390I CCNGG 1 cut(s) 50
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 165, 184
BpiI GAAGAC 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 58
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 71
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 88
BseXI GCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 1 cut(s) 66
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 1 cut(s) 285
BsrI ACTGG 1 cut(s) 24
BssMI GATC 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 202, 270
BstAPI GCANNNNNTGC 1 cut(s) 203
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 216
BstKTI GATC 1 cut(s) 288
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 1 cut(s) 285
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 264
BsuI GTATCC 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 216
Csp6I GTAC 2 cut(s) 27, 221
CviAII CATG 1 cut(s) 66
CviJI RGCY 3 cut(s) 23, 83, 124
CviKI_1 RGCY 3 cut(s) 23, 83, 124
CviQI GTAC 2 cut(s) 27, 221
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
Eco72I CACGTG 1 cut(s) 58
FaeI CATG 1 cut(s) 69
FaiI YATR 3 cut(s) 40, 42, 67
FaqI GGGAC 1 cut(s) 99
FatI CATG 1 cut(s) 65
FauNDI CATATG 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 182
Fsp4HI GCNGC 1 cut(s) 182
FspBI CTAG 2 cut(s) 191, 289
GluI GCNGC 1 cut(s) 182
HapII CCGG 1 cut(s) 50
Hin1II CATG 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 1 cut(s) 129
HpaII CCGG 1 cut(s) 50
Hpy188I TCNGA 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 2 cut(s) 202, 270
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 3 cut(s) 44, 197, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 1 cut(s) 69
Kzo9I GATC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 2 cut(s) 37, 63
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 2 cut(s) 165, 184
MaeI CTAG 2 cut(s) 191, 289
MaeII ACGT 1 cut(s) 57
MalI GATC 1 cut(s) 287
MboI GATC 1 cut(s) 285
MboII GAAGA 2 cut(s) 264, 267
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 2 cut(s) 33, 248
MnlI CCTC 3 cut(s) 109, 145, 251
MseI TTAA 1 cut(s) 32
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 232
MwoI GCNNNNNNNGC 1 cut(s) 203
NciI CCSGG 1 cut(s) 50
NdeI CATATG 1 cut(s) 40
NdeII GATC 1 cut(s) 285
NlaIII CATG 1 cut(s) 69
PctI GAATGC 1 cut(s) 232
PfeI GAWTC 1 cut(s) 129
PkrI GCNGC 1 cut(s) 183
PmaCI CACGTG 1 cut(s) 58
PmlI CACGTG 1 cut(s) 58
Ppu21I YACGTR 1 cut(s) 58
PshBI ATTAAT 1 cut(s) 32
PspCI CACGTG 1 cut(s) 58
RsaI GTAC 2 cut(s) 28, 222
RsaNI GTAC 2 cut(s) 27, 221
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 1 cut(s) 182
Sau3AI GATC 1 cut(s) 285
ScaI AGTACT 1 cut(s) 222
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 57
SetI ASST 4 cut(s) 60, 85, 192, 243
SfaNI GCATC 2 cut(s) 165, 184
Sse9I AATT 2 cut(s) 33, 248
SspMI CTAG 2 cut(s) 191, 289
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 202, 270
TaiI ACGT 1 cut(s) 60
TaqI TCGA 2 cut(s) 120, 234
TasI AATT 2 cut(s) 33, 248
TatI WGTACW 2 cut(s) 26, 220
TfiI GAWTC 1 cut(s) 129
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 1 cut(s) 166
TseI GCWGC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 166
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 1 cut(s) 248
XspI CTAG 2 cut(s) 191, 289
ZrmI AGTACT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.