Rmu_sc0001149.1_g000017

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001149.1
OG0022414
Physical Location & Seq
Reverse (-)
46824 .. 48602
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001149.1_g000017.1.cds

Sequence Viewer

Length: 480 bp
atgattggtcgtcgtcttgagcccggaaaaaatccgtttcttccaaaggatgaggatgaaaagttattagaggccgaagtaccctatctaagtgcaataggcgcattgttgtacttagctcaattaatgcacaagaccggacatctcattcgcaatgaacttgaccccaagaggcaaattgctgccatccataacatgaccgtcgaccatgctaccgccgtcggcgccaccgtcgtccaagcaggccgatgtcctcctccttccctccatcaaaatgacaatgatgttttgatgggtttttctaatgcagggtacctctctgaccctcacaaaggtctcgtcgagccaagattctcggaatggttggtatttgagggcatcagtgttgatgagagtgggaagcagcattacctagatgcaactgttgcttacaagcgtgcagtactgaatgccatcgactacctctccaaatttggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

159

Amino Acids

17.59

Weight (kDa)

5.94

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022414)

Species Orthologous Gene IDs
malus_domestica MD00G1015600.v1.1
rosa_multiflora Rmu_sc0001149.1_g000017
rosa_samantha Rh2BG208000 Rh2CG200800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 2 cut(s) 224, 312
AccI GTMKAC 1 cut(s) 204
AciI CCGC 1 cut(s) 216
AcsI RAATTY 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 4 cut(s) 81, 113, 314, 444
AfiI CCNNNNNNNGG 1 cut(s) 332
AluBI AGCT 1 cut(s) 119
AluI AGCT 1 cut(s) 119
Alw26I GTCTC 1 cut(s) 341
AoxI GGCC 2 cut(s) 72, 244
ApeKI GCWGC 2 cut(s) 182, 403
ApoI RAATTY 1 cut(s) 470
ArsI GACNNNNNNTTYG 4 cut(s) 132, 164, 324, 356
AseI ATTAAT 1 cut(s) 125
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 2 cut(s) 104, 227
AsuC2I CCSGG 1 cut(s) 24
BanI GGYRCC 2 cut(s) 224, 312
BanII GRGCYC 1 cut(s) 24
BarI GAAGNNNNNNTAC 2 cut(s) 392, 424
BbvI GCAGC 2 cut(s) 169, 415
BccI CCATC 4 cut(s) 194, 276, 286, 461
BceAI ACGGC 1 cut(s) 203
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 341
BfaI CTAG 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 228
BisI GCNGC 2 cut(s) 183, 404
BlsI GCNGC 2 cut(s) 184, 405
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 1 cut(s) 24
BmiI GGNNCC 2 cut(s) 226, 314
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 2 cut(s) 387, 406
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 341
BsaWI WCCGGW 1 cut(s) 137
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 160
BseGI GGATG 3 cut(s) 55, 61, 186
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 160
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 2 cut(s) 169, 415
BsgI GTGCAG 1 cut(s) 459
BshFI GGCC 2 cut(s) 74, 246
BshNI GGYRCC 2 cut(s) 224, 312
BsiSI CCGG 2 cut(s) 24, 138
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 341
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 2 cut(s) 74, 246
Bso31I GGTCTC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 24
BspACI CCGC 1 cut(s) 216
BspANI GGCC 2 cut(s) 74, 246
BspLI GGNNCC 2 cut(s) 226, 314
BspT107I GGYRCC 2 cut(s) 224, 312
BspTNI GGTCTC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 202, 232, 424
BstACI GRCGYC 1 cut(s) 225
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 244, 438
BstDEI CTNAG 2 cut(s) 89, 115
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 55, 61, 186
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 2 cut(s) 104, 227
BstMAI GTCTC 1 cut(s) 341
BstMWI GCNNNNNNNGC 3 cut(s) 101, 224, 425
BstSCI CCNGG 1 cut(s) 22
BstV1I GCAGC 2 cut(s) 169, 415
BsuRI GGCC 2 cut(s) 74, 246
BtsCI GGATG 3 cut(s) 55, 61, 186
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 2 cut(s) 244, 438
CfoI GCGC 2 cut(s) 104, 227
Csp6I GTAC 4 cut(s) 80, 112, 313, 443
CviAII CATG 2 cut(s) 196, 209
CviJI RGCY 5 cut(s) 22, 74, 119, 246, 346
CviKI_1 RGCY 5 cut(s) 22, 74, 119, 246, 346
CviQI GTAC 4 cut(s) 80, 112, 313, 443
DdeI CTNAG 2 cut(s) 89, 115
DinI GGCGCC 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 341
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 24
EgeI GGCGCC 1 cut(s) 226
EheI GGCGCC 1 cut(s) 226
FaeI CATG 2 cut(s) 199, 212
FaiI YATR 3 cut(s) 192, 197, 210
FatI CATG 2 cut(s) 195, 208
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 183, 404
FokI GGATG 3 cut(s) 62, 68, 173
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 2 cut(s) 183, 404
FspBI CTAG 1 cut(s) 413
GlaI GCGC 2 cut(s) 103, 226
GluI GCNGC 2 cut(s) 183, 404
HaeII RGCGCY 1 cut(s) 228
HaeIII GGCC 2 cut(s) 74, 246
HapII CCGG 2 cut(s) 24, 138
HhaI GCGC 2 cut(s) 104, 227
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 2 cut(s) 199, 212
Hin6I GCGC 2 cut(s) 102, 225
HinP1I GCGC 2 cut(s) 102, 225
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 1 cut(s) 351
HpaII CCGG 2 cut(s) 24, 138
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 322, 358
Hpy188III TCNNGA 1 cut(s) 17
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 5 cut(s) 15, 206, 224, 236, 344
HpyAV CCTTC 1 cut(s) 270
HpyCH4III ACNGT 3 cut(s) 202, 232, 424
HpyCH4V TGCA 5 cut(s) 95, 130, 308, 419, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 224, 425
HpyF3I CTNAG 2 cut(s) 89, 115
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 2 cut(s) 199, 212
HspAI GCGC 2 cut(s) 102, 225
KasI GGCGCC 1 cut(s) 224
KpnI GGTACC 1 cut(s) 316
LpnPI CCDG 4 cut(s) 37, 151, 228, 294
Lsp1109I GCAGC 2 cut(s) 169, 415
LweI GCATC 2 cut(s) 387, 406
MaeI CTAG 1 cut(s) 413
MboII GAAGA 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 3 cut(s) 122, 177, 470
Mly113I GGCGCC 1 cut(s) 225
MseI TTAA 1 cut(s) 125
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 2 cut(s) 24, 138
MspR9I CCNGG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 454
MwoI GCNNNNNNNGC 3 cut(s) 101, 224, 425
NarI GGCGCC 1 cut(s) 225
NciI CCSGG 1 cut(s) 24
NlaIII CATG 2 cut(s) 199, 212
NlaIV GGNNCC 2 cut(s) 226, 314
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 351
PkrI GCNGC 2 cut(s) 184, 405
PluTI GGCGCC 1 cut(s) 228
PshBI ATTAAT 1 cut(s) 125
PspN4I GGNNCC 2 cut(s) 226, 314
RsaI GTAC 4 cut(s) 81, 113, 314, 444
RsaNI GTAC 4 cut(s) 80, 112, 313, 443
RseI CAYNNNNRTG 1 cut(s) 273
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 2 cut(s) 183, 404
ScaI AGTACT 1 cut(s) 444
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 24
SetI ASST 5 cut(s) 121, 318, 337, 414, 465
SfaNI GCATC 2 cut(s) 387, 406
SfoI GGCGCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 3 cut(s) 122, 177, 470
SsiI CCGC 1 cut(s) 216
SspDI GGCGCC 1 cut(s) 224
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 3 cut(s) 202, 232, 424
TaqI TCGA 3 cut(s) 204, 342, 456
TasI AATT 3 cut(s) 122, 177, 470
TatI WGTACW 2 cut(s) 111, 442
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 388
TseI GCWGC 2 cut(s) 182, 403
TspDTI ATGAA 2 cut(s) 72, 171
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 1 cut(s) 388
VspI ATTAAT 1 cut(s) 125
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 413
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.