Rh2BG208000

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0022414
Physical Location & Seq
Forward (+)
19418954 .. 19419424
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG208000.1

Sequence Viewer

Length: 378 bp
ATGAAAGAATACCTTACACCGACGGGGCCAACTCCTCTTCATGTGAATCCAATCTTTCAAATAGGTCCCGTCGAGCCAAGATTCTGGGAATGGTTGGTATTTGAGGGCATCAGTGTTGATGAGAGTGGGAAGCAGCATTACCTAGATGCAACTGTTGCTTACAAGCGTGCAGTACTGAATGCCATCGACTACCTCTCCAAATTTGGATACTCAAAAGAACAGAGCTCCCTTCTGCTGTCATGCTGTCCATGTGAGGGAAGAATTTCTGGAATAGTCGACGCTCCCAATGCTGTTGCAACCCTAGCAATCCCAACAGCTATCTTTGACCAGTTATCTTTTTCTGGCCAAGATTCCAACAAAAACATACTATTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

125

Amino Acids

13.78

Weight (kDa)

5.0

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 16 - 110 1e-40 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022414)

Species Orthologous Gene IDs
malus_domestica MD00G1015600.v1.1
rosa_multiflora Rmu_sc0001149.1_g000017
rosa_samantha Rh2BG208000 Rh2CG200800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 2 cut(s) 200, 261
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 1 cut(s) 59
AluBI AGCT 2 cut(s) 225, 317
AluI AGCT 2 cut(s) 225, 317
Alw21I GWGCWC 1 cut(s) 227
AoxI GGCC 2 cut(s) 26, 343
ApeKI GCWGC 1 cut(s) 133
ApoI RAATTY 2 cut(s) 200, 261
Asp700I GAANNNNTTC 1 cut(s) 262
AspS9I GGNCC 2 cut(s) 26, 65
AvaII GGWCC 1 cut(s) 65
BalI TGGCCA 1 cut(s) 345
BanII GRGCYC 1 cut(s) 227
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 145
BccI CCATC 1 cut(s) 191
BciVI GTATCC 1 cut(s) 200
BfaI CTAG 2 cut(s) 143, 302
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 135
BmcAI AGTACT 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 26, 65
BmiI GGNNCC 2 cut(s) 27, 67
BmsI GCATC 2 cut(s) 117, 136
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 328
BseLI CCNNNNNNNGG 1 cut(s) 254
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 2 cut(s) 28, 345
BsiHKAI GWGCWC 1 cut(s) 227
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 51
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 2 cut(s) 28, 345
Bsp1286I GDGCHC 1 cut(s) 227
BspANI GGCC 2 cut(s) 28, 345
BspLI GGNNCC 2 cut(s) 27, 67
BsrI ACTGG 1 cut(s) 328
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 168
BstMWI GCNNNNNNNGC 3 cut(s) 155, 287, 302
BstV1I GCAGC 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 84
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 2 cut(s) 28, 345
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 26, 65
CseI GACGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 173
CviAII CATG 3 cut(s) 41, 240, 249
CviJI RGCY 5 cut(s) 28, 76, 225, 317, 345
CviKI_1 RGCY 5 cut(s) 28, 76, 225, 317, 345
CviQI GTAC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 65
Eco53kI GAGCTC 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 3 cut(s) 44, 243, 252
FaiI YATR 4 cut(s) 42, 241, 250, 365
FalI AAGNNNNNCTT 1 cut(s) 29
FaqI GGGAC 1 cut(s) 51
FatI CATG 3 cut(s) 40, 239, 248
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 134
FspBI CTAG 2 cut(s) 143, 302
GluI GCNGC 1 cut(s) 134
HaeIII GGCC 2 cut(s) 28, 345
HgaI GACGC 1 cut(s) 287
Hin1II CATG 3 cut(s) 44, 243, 252
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 46, 81, 350
Hpy166II GTNNAC 1 cut(s) 277
Hpy188III TCNNGA 1 cut(s) 267
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 3 cut(s) 25, 74, 281
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 3 cut(s) 149, 170, 296
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 287, 302
Hsp92II CATG 3 cut(s) 44, 243, 252
LmnI GCTCC 2 cut(s) 230, 286
LpnPI CCDG 4 cut(s) 70, 252, 327, 341
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 2 cut(s) 117, 136
MaeI CTAG 2 cut(s) 143, 302
MboII GAAGA 2 cut(s) 29, 270
MhlI GDGCHC 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 2 cut(s) 200, 261
MluNI TGGCCA 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 4 cut(s) 45, 97, 203, 247
Mox20I TGGCCA 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 262
MscI TGGCCA 1 cut(s) 345
Msp20I TGGCCA 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 184
MwoI GCNNNNNNNGC 3 cut(s) 155, 287, 302
NlaIII CATG 3 cut(s) 44, 243, 252
NlaIV GGNNCC 2 cut(s) 27, 67
PctI GAATGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 262
PfeI GAWTC 3 cut(s) 46, 81, 350
PkrI GCNGC 1 cut(s) 135
PpuMI RGGWCCY 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 65
PspN4I GGNNCC 2 cut(s) 27, 67
PspPI GGNCC 2 cut(s) 26, 65
PspPPI RGGWCCY 1 cut(s) 65
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
SacI GAGCTC 1 cut(s) 227
SalI GTCGAC 1 cut(s) 275
SatI GCNGC 1 cut(s) 134
Sau96I GGNCC 2 cut(s) 26, 65
ScaI AGTACT 1 cut(s) 174
SduI GDGCHC 1 cut(s) 227
SetI ASST 6 cut(s) 15, 67, 144, 195, 227, 319
SfaNI GCATC 2 cut(s) 117, 136
SinI GGWCC 1 cut(s) 65
Sse9I AATT 2 cut(s) 200, 261
SspMI CTAG 2 cut(s) 143, 302
SstI GAGCTC 1 cut(s) 227
TaaI ACNGT 1 cut(s) 154
TaqI TCGA 3 cut(s) 72, 186, 276
TasI AATT 2 cut(s) 200, 261
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 3 cut(s) 46, 81, 350
TscAI CASTG 1 cut(s) 118
TseI GCWGC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 17, 29
TspRI CASTG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 65
XapI RAATTY 2 cut(s) 200, 261
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 262
XspI CTAG 2 cut(s) 143, 302
ZrmI AGTACT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.