Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0015852

Physical Location & Seq

Genomic Coordinates Forward (+)

5675721 .. 5678340

Gene Structure 2620 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1032400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGCATCTCCTTCTACTTTCTCTCCCATTTCCCTCTCTTCTCTTTTCCTTTTCCTTCTCCTCCTTCCACTTTCATCATCTGCATTGAACCCCGCCAGCGGCCACCTTCCTTTGTCTTCCCCCGCCTTCTCCGCGAAAGGCCAAGCTGAGAAGTTCATCAGGGCCCTCAACCTGTTCCCAAAGCATGATGTTAACATTCTGTCCCGTAAACCTTCATTGGATGCTGAGGCAGATGCTGGTGTTGAGATTGTGGAGAAGCAGTTCAAAATGCCTTTTCTTGACGCCGCTTCCGGTCCTTCCGCTAAAGATCTTGGTCACCACGCCGGCTACTACCGCCTTCCCCATTCGGAAGGTGCAAGGTTGTTCTACTTATTTTTCGAATCAAGGATTAACAAGAACGACCCTGTTGTGATATGGTTGACCGGAGGGCCGGGATGTAGCAGTGAACTGGCTCTGTTCTATGAAAATGGTCCTTTTCACATTCAAAACAACTTGTCACTCACTTGGAATAACTATGGCTGGGACAAGGCATCAAACATTCTTTACGTCGACCAACCTGTTGGAACTGGTTTCAGCTATACTACAAATTCGAGTGACATTCCTCATGATGAAGCAGGCGTCAGCAACGACTTATATGATTTTCTGCAGGGATTTTTCGCTCTACATCCTGAATTCGCGAAAAATGACTTCTACATCACCGGAGAGTCATATGCTGGGCACTATGTTCCCGCGCTTGCTTCTCGGGTTAACAAAGGAAATAAAGCAAAGGAAGGGAATCACATAAACCTCAAGGGTTTCGCCATAGGTAATGGGTTGACCAATCCGGGAATCCAGTACAAGGCATACACTGATTATGCACTGCAAATGAAGTTGATTACTAAAGCAGACTACGACGCTATATCGCAGATAATTCCAGACTGTGAAGAGTCAGTGAAGGCTTGCGACGCCTCTAAGAGTGGAGATGCTTGTGAGGAGTCGTTAAGTATTTGCAACAGCATATTTAGCCAGATAATGCAAAGAAATAGCGGTATAAATTACTATGATATTAGGAAGCAATGTGAGGGGAGCTTGTGCTATGACTTCTCAAACATGGAGACATTCTTGAACAAACAAACGGTTAGGGATGCCCTAGGAGTTGGGGACATCGAATTTGTGTCATGCAGCTCTGTGGTATATGATGCTATGCTGAATGACTGGATGAGAAATCTGGAAGTGGGAATTCCTGCCCTCCTTGAAGATGGAATAAAGTTGCTTGTGTATGCTGGGGAGTATGATCTCATCTGCAATTGGCTTGGGAATTCAAATTGGGCTCATGCCATGGAATGGTCCGGTCAGAAAGCATTCGTAGCAGCACAAACTGTTCCATTCAAGGTTGCGGGTGCAGAAGCAGGACTGCTAAAAAGCTATGGACCTCTAGCTTTCCTCAAGGTTCATAATGCTGGTCATATGGTTCCAATGGATCAACCGGAAGCTGCACTACAGATGCTTACAAACTGGATGCAAGGAAATCTGGCCGTGGCTGAGTCAGCCAAAAGAGTTGCTCCCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0017171 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070008 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

517

Amino Acids

56.54

Weight (kDa)

5.26

Isoelectric Point (pI)

43.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	103 - 502	1.1e-129	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015852)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45010
malus_domestica	MD00G1032400.v1.1 MD11G1146500.v1.1
prunus_persica	Prupe.6G109600_v2.0.a1
pyrus_communis	pycom11g11950
rosa_chinensis	RchiOBHm_Chr5g0057441
rosa_laevigata	RLG00000035154
rosa_multiflora	Rmu_sc0003730.1_g000012
rosa_roxburghii	Rroxscaffold_1G00023010
rosa_rugosa	Rorug05G0307200 Rorug05G0307300
rosa_samantha	Rh5AG375800 Rh5BG386600 Rh5DG400900
rosa_wichuraiana	Rw5G035480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1323
AccI	GTMKAC	1 cut(s)	549
AccII	CGCG	3 cut(s)	134, 677, 731
AclWI	GGATC	1 cut(s)	1467
AcoI	YGGCCR	2 cut(s)	100, 1512
AcsI	RAATTY	5 cut(s)	586, 671, 1148, 1218, 1297
AcyI	GRCGYC	3 cut(s)	282, 618, 945
AfaI	GTAC	1 cut(s)	836
AfiI	CCNNNNNNNGG	3 cut(s)	98, 838, 1323
AgsI	TTSAA	7 cut(s)	88, 265, 485, 1105, 1235, 1302, 1369
AloI	GAACNNNNNNTCC	2 cut(s)	245, 277
AluBI	AGCT	7 cut(s)	146, 576, 1068, 1164, 1404, 1418, 1472
AluI	AGCT	7 cut(s)	146, 576, 1068, 1164, 1404, 1418, 1472
Alw26I	GTCTC	1 cut(s)	1088
AlwI	GGATC	1 cut(s)	1467
AlwNI	CAGNNNCTG	1 cut(s)	236
Ama87I	CYCGRG	1 cut(s)	741
AoxI	GGCC	5 cut(s)	100, 139, 162, 428, 1512
ApaI	GGGCCC	1 cut(s)	166
ApeKI	GCWGC	3 cut(s)	1161, 1349, 1472
ApoI	RAATTY	5 cut(s)	586, 671, 1148, 1218, 1297
ArsI	GACNNNNNNTTYG	2 cut(s)	1133, 1165
Asp700I	GAANNNNTTC	2 cut(s)	260, 1340
AspA2I	CCTAGG	1 cut(s)	1129
AspLEI	GCGC	1 cut(s)	733
AspS9I	GGNCC	7 cut(s)	162, 163, 293, 428, 470, 1326, 1409
AsuC2I	CCSGG	2 cut(s)	432, 825
AsuHPI	GGTGA	2 cut(s)	308, 688
AsuII	TTCGAA	1 cut(s)	378
AvaI	CYCGRG	1 cut(s)	741
AvaII	GGWCC	4 cut(s)	293, 470, 1326, 1409
AvrII	CCTAGG	1 cut(s)	1129
BaeGI	GKGCMC	2 cut(s)	166, 720
BanII	GRGCYC	2 cut(s)	166, 1312
BarI	GAAGNNNNNNTAC	2 cut(s)	1461, 1493
BbsI	GAAGAC	1 cut(s)	108
BbvCI	CCTCAGC	1 cut(s)	225
BbvI	GCAGC	3 cut(s)	1173, 1361, 1459
BccI	CCATC	1 cut(s)	1232
BceAI	ACGGC	1 cut(s)	1499
BcnI	CCSGG	2 cut(s)	432, 825
BcoDI	GTCTC	1 cut(s)	1088
BfaI	CTAG	2 cut(s)	1130, 1415
BfmI	CTRYAG	2 cut(s)	644, 1478
BglII	AGATCT	1 cut(s)	307
BisI	GCNGC	5 cut(s)	100, 285, 1162, 1350, 1473
BlnI	CCTAGG	1 cut(s)	1129
BlsI	GCNGC	5 cut(s)	101, 286, 1163, 1351, 1474
Bme1390I	CCNGG	2 cut(s)	432, 825
Bme18I	GGWCC	4 cut(s)	293, 470, 1326, 1409
BmeT110I	CYCGRG	1 cut(s)	741
BmgT120I	GGNCC	7 cut(s)	162, 163, 293, 428, 470, 1326, 1409
BmiI	GGNNCC	2 cut(s)	164, 1452
BmrFI	CCNGG	2 cut(s)	432, 825
BmsI	GCATC	9 cut(s)	14, 211, 223, 539, 952, 1114, 1168, 1473, 1488
BpiI	GAAGAC	1 cut(s)	108
Bpu10I	CCTNAGC	1 cut(s)	225
Bpu14I	TTCGAA	1 cut(s)	378
BpuEI	CTTGAG	2 cut(s)	773, 1409
BpuMI	CCSGG	2 cut(s)	432, 825
BsaHI	GRCGYC	3 cut(s)	282, 618, 945
BsaJI	CCNNGG	3 cut(s)	1129, 1317, 1515
BsaWI	WCCGGW	5 cut(s)	290, 422, 698, 1328, 1465
BsaXI	ACNNNNNCTCC	4 cut(s)	7, 37, 245, 275
Bsc4I	CCNNNNNNNGG	3 cut(s)	98, 838, 1323
Bse118I	RCCGGY	1 cut(s)	323
Bse1I	ACTGG	5 cut(s)	453, 571, 832, 1199, 1499
Bse3DI	GCAATG	1 cut(s)	1061
BseDI	CCNNGG	3 cut(s)	1129, 1317, 1515
BseGI	GGATG	6 cut(s)	226, 440, 664, 1129, 1203, 1503
BseLI	CCNNNNNNNGG	3 cut(s)	98, 838, 1323
BseMI	GCAATG	1 cut(s)	1061
BseMII	CTCAG	3 cut(s)	138, 216, 1512
BseNI	ACTGG	5 cut(s)	453, 571, 832, 1199, 1499
BseRI	GAGGAG	2 cut(s)	50, 986
BseSI	GKGCMC	2 cut(s)	166, 720
BseXI	GCAGC	3 cut(s)	1173, 1361, 1459
BseYI	CCCAGC	3 cut(s)	519, 713, 1262
BsgI	GTGCAG	2 cut(s)	1401, 1458
Bsh1236I	CGCG	3 cut(s)	134, 677, 731
BshFI	GGCC	5 cut(s)	102, 141, 164, 430, 1514
BsiHKCI	CYCGRG	1 cut(s)	741
BsiSI	CCGG	8 cut(s)	291, 324, 423, 431, 699, 824, 1329, 1466
BslFI	GGGAC	3 cut(s)	187, 536, 1154
BslI	CCNNNNNNNGG	3 cut(s)	98, 838, 1323
BsmAI	GTCTC	1 cut(s)	1088
BsmFI	GGGAC	3 cut(s)	187, 536, 1154
BsmI	GAATGC	1 cut(s)	1340
BsnI	GGCC	5 cut(s)	102, 141, 164, 430, 1514
BsoBI	CYCGRG	1 cut(s)	741
Bsp119I	TTCGAA	1 cut(s)	378
Bsp120I	GGGCCC	1 cut(s)	162
Bsp1286I	GDGCHC	3 cut(s)	166, 720, 1312
Bsp143I	GATC	3 cut(s)	307, 1273, 1459
Bsp19I	CCATGG	1 cut(s)	1317
Bsp68I	TCGCGA	1 cut(s)	677
BspANI	GGCC	5 cut(s)	102, 141, 164, 430, 1514
BspCNI	CTCAG	3 cut(s)	139, 217, 1513
BspFNI	CGCG	3 cut(s)	134, 677, 731
BspHI	TCATGA	1 cut(s)	604
BspLI	GGNNCC	2 cut(s)	164, 1452
BspMAI	CTGCAG	1 cut(s)	648
BspPI	GGATC	1 cut(s)	1467
BspT104I	TTCGAA	1 cut(s)	378
BsrDI	GCAATG	1 cut(s)	1061
BsrFI	RCCGGY	1 cut(s)	323
BsrI	ACTGG	5 cut(s)	453, 571, 832, 1199, 1499
BssAI	RCCGGY	1 cut(s)	323
BssECI	CCNNGG	3 cut(s)	1129, 1317, 1515
BssMI	GATC	3 cut(s)	307, 1273, 1459
BssNI	GRCGYC	3 cut(s)	282, 618, 945
BssT1I	CCWWGG	2 cut(s)	1129, 1317
Bst4CI	ACNGT	3 cut(s)	920, 1117, 1360
Bst6I	CTCTTC	2 cut(s)	43, 918
BstACI	GRCGYC	3 cut(s)	282, 618, 945
BstBI	TTCGAA	1 cut(s)	378
BstC8I	GCNNGC	5 cut(s)	97, 325, 616, 735, 940
BstDEI	CTNAG	4 cut(s)	147, 225, 951, 1521
BstDSI	CCRYGG	2 cut(s)	1317, 1515
BstEII	GGTNACC	1 cut(s)	314
BstF5I	GGATG	6 cut(s)	226, 440, 664, 1129, 1203, 1503
BstFNI	CGCG	3 cut(s)	134, 677, 731
BstHHI	GCGC	1 cut(s)	733
BstKTI	GATC	3 cut(s)	310, 1276, 1462
BstMAI	GTCTC	1 cut(s)	1088
BstMBI	GATC	3 cut(s)	307, 1273, 1459
BstMWI	GCNNNNNNNGC	6 cut(s)	131, 333, 944, 1002, 1346, 1526
BstPI	GGTNACC	1 cut(s)	314
BstSCI	CCNGG	2 cut(s)	430, 823
BstSFI	CTRYAG	2 cut(s)	644, 1478
BstSLI	GKGCMC	2 cut(s)	166, 720
BstUI	CGCG	3 cut(s)	134, 677, 731
BstV1I	GCAGC	3 cut(s)	1173, 1361, 1459
BstV2I	GAAGAC	1 cut(s)	108
BstX2I	RGATCY	1 cut(s)	307
BstXI	CCANNNNNNTGG	1 cut(s)	560
BstYI	RGATCY	1 cut(s)	307
BsuRI	GGCC	5 cut(s)	102, 141, 164, 430, 1514
BtgI	CCRYGG	2 cut(s)	1317, 1515
BtsCI	GGATG	6 cut(s)	226, 440, 664, 1129, 1203, 1503
BtsI	GCAGTG	2 cut(s)	448, 857
BtsIMutI	CAGTG	4 cut(s)	448, 846, 857, 936
BtuMI	TCGCGA	1 cut(s)	677
Cac8I	GCNNGC	5 cut(s)	97, 325, 616, 735, 940
CaiI	CAGNNNCTG	1 cut(s)	236
CciI	TCATGA	1 cut(s)	604
CfoI	GCGC	1 cut(s)	733
Cfr10I	RCCGGY	1 cut(s)	323
Cfr13I	GGNCC	7 cut(s)	162, 163, 293, 428, 470, 1326, 1409
CseI	GACGC	4 cut(s)	290, 607, 902, 953
Csp6I	GTAC	1 cut(s)	835
CviAII	CATG	6 cut(s)	185, 605, 1090, 1158, 1313, 1318
CviQI	GTAC	1 cut(s)	835
DdeI	CTNAG	4 cut(s)	147, 225, 951, 1521
DpnI	GATC	3 cut(s)	309, 1275, 1461
DpnII	GATC	3 cut(s)	307, 1273, 1459
EaeI	YGGCCR	2 cut(s)	100, 1512
Eam1104I	CTCTTC	2 cut(s)	43, 918
EarI	CTCTTC	2 cut(s)	43, 918
Eco130I	CCWWGG	2 cut(s)	1129, 1317
Eco24I	GRGCYC	2 cut(s)	166, 1312
Eco47I	GGWCC	4 cut(s)	293, 470, 1326, 1409
Eco88I	CYCGRG	1 cut(s)	741
Eco91I	GGTNACC	1 cut(s)	314
EcoO109I	RGGNCCY	2 cut(s)	162, 163
EcoO65I	GGTNACC	1 cut(s)	314
EcoRI	GAATTC	3 cut(s)	671, 1218, 1297
EcoT14I	CCWWGG	2 cut(s)	1129, 1317
EcoT38I	GRGCYC	2 cut(s)	166, 1312
ErhI	CCWWGG	2 cut(s)	1129, 1317
FaeI	CATG	6 cut(s)	188, 608, 1093, 1161, 1316, 1321
FaqI	GGGAC	3 cut(s)	187, 536, 1154
FatI	CATG	6 cut(s)	184, 604, 1089, 1157, 1312, 1317
FauI	CCCGC	4 cut(s)	100, 130, 736, 1369
FauNDI	CATATG	2 cut(s)	709, 1446
FblI	GTMKAC	1 cut(s)	549
Fnu4HI	GCNGC	5 cut(s)	100, 285, 1162, 1350, 1473
FokI	GGATG	6 cut(s)	233, 447, 651, 1136, 1210, 1510
FriOI	GRGCYC	2 cut(s)	166, 1312
Fsp4HI	GCNGC	5 cut(s)	100, 285, 1162, 1350, 1473
FspBI	CTAG	2 cut(s)	1130, 1415
GlaI	GCGC	1 cut(s)	732
GluI	GCNGC	5 cut(s)	100, 285, 1162, 1350, 1473
GsaI	CCCAGC	3 cut(s)	523, 717, 1266
HaeIII	GGCC	5 cut(s)	102, 141, 164, 430, 1514
HapII	CCGG	8 cut(s)	291, 324, 423, 431, 699, 824, 1329, 1466
HgaI	GACGC	4 cut(s)	290, 607, 902, 953
HhaI	GCGC	1 cut(s)	733
Hin1I	GRCGYC	3 cut(s)	282, 618, 945
Hin1II	CATG	6 cut(s)	188, 608, 1093, 1161, 1316, 1321
Hin6I	GCGC	1 cut(s)	731
HinP1I	GCGC	1 cut(s)	731
HincII	GTYRAC	5 cut(s)	193, 420, 550, 748, 816
HindII	GTYRAC	5 cut(s)	193, 420, 550, 748, 816
HinfI	GANTC	7 cut(s)	380, 704, 775, 828, 926, 974, 1523
HpaI	GTTAAC	2 cut(s)	193, 748
HpaII	CCGG	8 cut(s)	291, 324, 423, 431, 699, 824, 1329, 1466
HphI	GGTGA	2 cut(s)	308, 688
Hpy166II	GTNNAC	7 cut(s)	193, 209, 420, 446, 550, 748, 816
Hpy188I	TCNGA	2 cut(s)	349, 1335
Hpy188III	TCNNGA	7 cut(s)	278, 605, 668, 676, 914, 1102, 1208
Hpy8I	GTNNAC	7 cut(s)	193, 209, 420, 446, 550, 748, 816
Hpy99I	CGWCG	3 cut(s)	551, 896, 947
HpyCH4III	ACNGT	3 cut(s)	920, 1117, 1360
HpyCH4IV	ACGT	1 cut(s)	546
HpyF10VI	GCNNNNNNNGC	6 cut(s)	131, 333, 944, 1002, 1346, 1526
HpyF3I	CTNAG	4 cut(s)	147, 225, 951, 1521
HpySE526I	ACGT	1 cut(s)	546
Hsp92I	GRCGYC	3 cut(s)	282, 618, 945
Hsp92II	CATG	6 cut(s)	188, 608, 1093, 1161, 1316, 1321
HspAI	GCGC	1 cut(s)	731
KroI	GCCGGC	1 cut(s)	323
KroNI	GCCGGC	1 cut(s)	325
KspAI	GTTAAC	2 cut(s)	193, 748
Kzo9I	GATC	3 cut(s)	307, 1273, 1459
LmnI	GCTCC	2 cut(s)	1065, 1546
Lsp1109I	GCAGC	3 cut(s)	1173, 1361, 1459
LweI	GCATC	9 cut(s)	14, 211, 223, 539, 952, 1114, 1168, 1473, 1488
MaeI	CTAG	2 cut(s)	1130, 1415
MaeII	ACGT	1 cut(s)	546
MaeIII	GTNAC	3 cut(s)	314, 495, 593
MalI	GATC	3 cut(s)	309, 1275, 1461
MboI	GATC	3 cut(s)	307, 1273, 1459
MboII	GAAGA	4 cut(s)	30, 108, 935, 1247
MfeI	CAATTG	2 cut(s)	1285, 1546
MflI	RGATCY	1 cut(s)	307
MhlI	GDGCHC	3 cut(s)	166, 720, 1312
MlyI	GAGTC	4 cut(s)	713, 935, 983, 1532
MmeI	TCCRAC	1 cut(s)	541
MroNI	GCCGGC	1 cut(s)	323
MroXI	GAANNNNTTC	2 cut(s)	260, 1340
MseI	TTAA	4 cut(s)	192, 390, 747, 980
MspA1I	CMGCKG	1 cut(s)	99
MspI	CCGG	8 cut(s)	291, 324, 423, 431, 699, 824, 1329, 1466
MspR9I	CCNGG	2 cut(s)	432, 825
MunI	CAATTG	2 cut(s)	1285, 1546
Mva1269I	GAATGC	1 cut(s)	1340
MvnI	CGCG	3 cut(s)	134, 677, 731
MwoI	GCNNNNNNNGC	6 cut(s)	131, 333, 944, 1002, 1346, 1526
NaeI	GCCGGC	1 cut(s)	325
NciI	CCSGG	2 cut(s)	432, 825
NcoI	CCATGG	1 cut(s)	1317
NdeI	CATATG	2 cut(s)	709, 1446
NdeII	GATC	3 cut(s)	307, 1273, 1459
NgoMIV	GCCGGC	1 cut(s)	323
NlaIII	CATG	6 cut(s)	188, 608, 1093, 1161, 1316, 1321
NlaIV	GGNNCC	2 cut(s)	164, 1452
NmuCI	GTSAC	3 cut(s)	314, 495, 593
NruI	TCGCGA	1 cut(s)	677
NspV	TTCGAA	1 cut(s)	378
PagI	TCATGA	1 cut(s)	604
PctI	GAATGC	1 cut(s)	1340
PdiI	GCCGGC	1 cut(s)	325
PdmI	GAANNNNTTC	2 cut(s)	260, 1340
PfeI	GAWTC	3 cut(s)	380, 775, 828
PflMI	CCANNNNNTGG	1 cut(s)	1323
PfoI	TCCNGGA	1 cut(s)	823
PkrI	GCNGC	5 cut(s)	101, 286, 1163, 1351, 1474
PleI	GAGTC	4 cut(s)	712, 934, 982, 1531
PpsI	GAGTC	4 cut(s)	712, 934, 982, 1531
PspEI	GGTNACC	1 cut(s)	314
PspFI	CCCAGC	3 cut(s)	519, 713, 1262
PspN4I	GGNNCC	2 cut(s)	164, 1452
PspOMI	GGGCCC	1 cut(s)	162
PspPI	GGNCC	7 cut(s)	162, 163, 293, 428, 470, 1326, 1409
PstI	CTGCAG	1 cut(s)	648
PstNI	CAGNNNCTG	1 cut(s)	236
PsuI	RGATCY	1 cut(s)	307
RruI	TCGCGA	1 cut(s)	677
RsaI	GTAC	1 cut(s)	836
RsaNI	GTAC	1 cut(s)	835
SalI	GTCGAC	1 cut(s)	548
SaqAI	TTAA	4 cut(s)	192, 390, 747, 980
SatI	GCNGC	5 cut(s)	100, 285, 1162, 1350, 1473
Sau3AI	GATC	3 cut(s)	307, 1273, 1459
Sau96I	GGNCC	7 cut(s)	162, 163, 293, 428, 470, 1326, 1409
SchI	GAGTC	4 cut(s)	713, 935, 983, 1532
ScrFI	CCNGG	2 cut(s)	432, 825
SduI	GDGCHC	3 cut(s)	166, 720, 1312
SfaNI	GCATC	9 cut(s)	14, 211, 223, 539, 952, 1114, 1168, 1473, 1488
SfcI	CTRYAG	2 cut(s)	644, 1478
SfuI	TTCGAA	1 cut(s)	378
SinI	GGWCC	4 cut(s)	293, 470, 1326, 1409
SmlI	CTYRAG	2 cut(s)	788, 1424
SmoI	CTYRAG	2 cut(s)	788, 1424
SspMI	CTAG	2 cut(s)	1130, 1415
StyD4I	CCNGG	2 cut(s)	430, 823
StyI	CCWWGG	2 cut(s)	1129, 1317
TaaI	ACNGT	3 cut(s)	920, 1117, 1360
TaiI	ACGT	1 cut(s)	549
TaqI	TCGA	4 cut(s)	378, 549, 590, 1146
TatI	WGTACW	1 cut(s)	834
TauI	GCSGC	2 cut(s)	102, 287
TfiI	GAWTC	3 cut(s)	380, 775, 828
Tru1I	TTAA	4 cut(s)	192, 390, 747, 980
Tru9I	TTAA	4 cut(s)	192, 390, 747, 980
TscAI	CASTG	4 cut(s)	448, 853, 864, 936
TseFI	GTSAC	3 cut(s)	314, 495, 593
TseI	GCWGC	3 cut(s)	1161, 1349, 1472
Tsp45I	GTSAC	3 cut(s)	314, 495, 593
TspDTI	ATGAA	7 cut(s)	63, 145, 204, 477, 624, 881, 1421
TspRI	CASTG	4 cut(s)	448, 853, 864, 936
Van91I	CCANNNNNTGG	1 cut(s)	1323
VpaK11BI	GGWCC	4 cut(s)	293, 470, 1326, 1409
XapI	RAATTY	5 cut(s)	586, 671, 1148, 1218, 1297
XmaJI	CCTAGG	1 cut(s)	1129
XmiI	GTMKAC	1 cut(s)	549
XmnI	GAANNNNTTC	2 cut(s)	260, 1340
XspI	CTAG	2 cut(s)	1130, 1415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.