MD01G1016600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0017958
Physical Location & Seq
Forward (+)
7583510 .. 7584088
579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1016600.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGCGGAGTACGAGAGGCATCAGCTGCAGCAACAGTCAGGCGGTGGCGGCGGAATCCCCAAAGAGAGAGCTGTGCAAGCCATAAACACAATCATACAGCTCCACTTCGAGAAAACCCTCGAGAAGAAGAGGTCCATCGACCTCCAGAAGAAGGAGCTCCACAAGCTCTTCATCCTCTTCTTCATCTTCCTCGCCCTCGTCTTCTCCGCCCAGTCCTCTGCCTCCTCACGCCTCCAGTGCCGCCACTGCTGGGTCCCAATCATCCTTCTCTCCCTGGCCCACCTCATCTTCTACGTCTCGGTGGCGCAGACGCTCCGGTGCATCAACGGGTTCAAGTACCAGCGGCGCTGCCACAAGCTGACGCTGGGACTCGCCACCGAGAAGCTGAGGGAGATGAAGGTGAGGCTGAGCGATGAATTCGATGCCCCGATTTCCGACGAGGAATTTGAGATTCACTACCAGGAGCCTCCGGAGGCCTACTTTGCGAAGTTCAAGAGGAACTGGGCTTGCCATTTTGCCTTCTTGATCCTCATCTACTGCTTCATGGTCTCTTCCTCTGTTGTTCTCCTCTGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

22.38

Weight (kDa)

8.97

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18800
fragaria_vesca FvH4_1g23610
malus_domestica MD01G1016600.v1.1
prunus_persica Prupe.6G162500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0119191
rosa_laevigata RLG00000018450
rosa_multiflora Rmu_sc0000184.1_g000011
rosa_roxburghii Rroxscaffold_2G00124460
rosa_rugosa Rorug02G0220800
rosa_samantha Rh2AG278700
rosa_wichuraiana Rw2G022150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 7 cut(s) 5, 42, 48, 51, 207, 241, 343
AclWI GGATC 1 cut(s) 520
AcsI RAATTY 2 cut(s) 416, 443
AfaI GTAC 2 cut(s) 11, 338
AfiI CCNNNNNNNGG 2 cut(s) 151, 250
AgsI TTSAA 2 cut(s) 334, 493
AjnI CCWGG 2 cut(s) 273, 459
AluBI AGCT 7 cut(s) 25, 71, 100, 157, 166, 358, 385
AluI AGCT 7 cut(s) 25, 71, 100, 157, 166, 358, 385
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 2 cut(s) 301, 553
AlwI GGATC 1 cut(s) 520
Ama87I CYCGRG 1 cut(s) 119
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 2 cut(s) 276, 474
ApeKI GCWGC 3 cut(s) 25, 28, 348
ApoI RAATTY 2 cut(s) 416, 443
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspLEI GCGC 2 cut(s) 307, 348
AspS9I GGNCC 3 cut(s) 132, 253, 277
AsuHPI GGTGA 1 cut(s) 412
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 2 cut(s) 132, 253
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 159
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 3 cut(s) 12, 40, 335
BccI CCATC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 275, 461
BcoDI GTCTC 2 cut(s) 301, 553
BfmI CTRYAG 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 349
BisI GCNGC 6 cut(s) 26, 29, 49, 241, 344, 349
BlpI GCTNAGC 1 cut(s) 407
BlsI GCNGC 6 cut(s) 27, 30, 50, 242, 345, 350
Bme1390I CCNGG 2 cut(s) 275, 461
Bme18I GGWCC 2 cut(s) 132, 253
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 3 cut(s) 132, 253, 277
BmiI GGNNCC 3 cut(s) 254, 255, 465
BmrFI CCNGG 2 cut(s) 275, 461
BmrI ACTGGG 2 cut(s) 205, 511
BmsI GCATC 3 cut(s) 28, 330, 412
BmuI ACTGGG 2 cut(s) 205, 511
BpiI GAAGAC 1 cut(s) 193
BpmI CTGGAG 2 cut(s) 128, 218
Bpu10I CCTNAGC 1 cut(s) 386
Bpu1102I GCTNAGC 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 530
BsaI GGTCTC 1 cut(s) 553
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 2 cut(s) 315, 469
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 250
Bse1I ACTGG 3 cut(s) 211, 235, 506
Bse8I GATNNNNATC 1 cut(s) 530
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 2 cut(s) 275, 461
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 2 cut(s) 171, 261
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 2 cut(s) 151, 250
BseMII CTCAG 2 cut(s) 377, 398
BseNI ACTGG 3 cut(s) 211, 235, 506
BseRI GAGGAG 2 cut(s) 214, 557
BseXI GCAGC 3 cut(s) 12, 40, 335
BseYI CCCAGC 2 cut(s) 249, 364
BshFI GGCC 2 cut(s) 278, 476
BsiHKAI GWGCWC 1 cut(s) 159
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 2 cut(s) 316, 470
BslFI GGGAC 2 cut(s) 239, 381
BslI CCNNNNNNNGG 2 cut(s) 151, 250
BsmAI GTCTC 2 cut(s) 301, 553
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 2 cut(s) 239, 381
BsnI GGCC 2 cut(s) 278, 476
Bso31I GGTCTC 1 cut(s) 553
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 159
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 1 cut(s) 525
Bsp1720I GCTNAGC 1 cut(s) 407
BspACI CCGC 7 cut(s) 5, 42, 48, 51, 207, 241, 343
BspANI GGCC 2 cut(s) 278, 476
BspCNI CTCAG 2 cut(s) 378, 399
BspEI TCCGGA 1 cut(s) 469
BspLI GGNNCC 3 cut(s) 254, 255, 465
BspMAI CTGCAG 1 cut(s) 30
BspPI GGATC 1 cut(s) 520
BspQI GCTCTTC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 553
BsrI ACTGG 3 cut(s) 211, 235, 506
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 1 cut(s) 525
Bst2UI CCWGG 2 cut(s) 275, 461
Bst4CI ACNGT 1 cut(s) 36
Bst6I CTCTTC 4 cut(s) 122, 173, 182, 556
BstAPI GCANNNNNTGC 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 78, 508
BstDEI CTNAG 2 cut(s) 386, 407
BstF5I GGATG 2 cut(s) 171, 261
BstH2I RGCGCY 1 cut(s) 349
BstHHI GCGC 2 cut(s) 307, 348
BstKTI GATC 1 cut(s) 528
BstMAI GTCTC 2 cut(s) 301, 553
BstMBI GATC 1 cut(s) 525
BstMWI GCNNNNNNNGC 7 cut(s) 25, 48, 77, 163, 237, 246, 482
BstNI CCWGG 2 cut(s) 275, 461
BstSCI CCNGG 2 cut(s) 273, 459
BstSFI CTRYAG 1 cut(s) 26
BstV1I GCAGC 3 cut(s) 12, 40, 335
BstV2I GAAGAC 1 cut(s) 193
BsuRI GGCC 2 cut(s) 278, 476
BtgZI GCGATG 1 cut(s) 426
BtsCI GGATG 2 cut(s) 171, 261
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 2 cut(s) 242, 244
Cac8I GCNNGC 2 cut(s) 78, 508
CfoI GCGC 2 cut(s) 307, 348
Cfr13I GGNCC 3 cut(s) 132, 253, 277
CseI GACGC 2 cut(s) 319, 370
Csp6I GTAC 2 cut(s) 10, 337
CviAII CATG 1 cut(s) 544
CviQI GTAC 2 cut(s) 10, 337
DdeI CTNAG 2 cut(s) 386, 407
DpnI GATC 1 cut(s) 527
DpnII GATC 1 cut(s) 525
Eam1104I CTCTTC 4 cut(s) 122, 173, 182, 556
EarI CTCTTC 4 cut(s) 122, 173, 182, 556
EciI GGCGGA 3 cut(s) 20, 66, 196
Ecl136II GAGCTC 1 cut(s) 157
Eco147I AGGCCT 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 132, 253
Eco53kI GAGCTC 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 253
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 273, 459
EcoT38I GRGCYC 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 1 cut(s) 547
FaiI YATR 3 cut(s) 83, 95, 545
FaqI GGGAC 2 cut(s) 239, 381
FatI CATG 1 cut(s) 543
Fnu4HI GCNGC 6 cut(s) 26, 29, 49, 241, 344, 349
FokI GGATG 2 cut(s) 158, 248
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 6 cut(s) 26, 29, 49, 241, 344, 349
GlaI GCGC 2 cut(s) 306, 347
GluI GCNGC 6 cut(s) 26, 29, 49, 241, 344, 349
GsaI CCCAGC 2 cut(s) 253, 368
GsuI CTGGAG 2 cut(s) 128, 218
HaeII RGCGCY 1 cut(s) 349
HaeIII GGCC 2 cut(s) 278, 476
HapII CCGG 2 cut(s) 316, 470
HgaI GACGC 2 cut(s) 319, 370
HhaI GCGC 2 cut(s) 307, 348
Hin1II CATG 1 cut(s) 547
Hin6I GCGC 2 cut(s) 305, 346
HinP1I GCGC 2 cut(s) 305, 346
HinfI GANTC 3 cut(s) 54, 369, 451
HpaII CCGG 2 cut(s) 316, 470
HphI GGTGA 1 cut(s) 412
Hpy188I TCNGA 1 cut(s) 436
Hpy188III TCNNGA 6 cut(s) 109, 121, 145, 470, 493, 523
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 4 cut(s) 145, 275, 391, 529
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 3 cut(s) 28, 76, 321
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 48, 77, 163, 237, 246, 482
HpyF3I CTNAG 2 cut(s) 386, 407
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 1 cut(s) 547
HspAI GCGC 2 cut(s) 305, 346
KflI GGGWCCC 1 cut(s) 253
Kpn2I TCCGGA 1 cut(s) 469
Kzo9I GATC 1 cut(s) 525
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 5 cut(s) 105, 154, 162, 318, 463
Lsp1109I GCAGC 3 cut(s) 12, 40, 335
LweI GCATC 3 cut(s) 28, 330, 412
MaeII ACGT 1 cut(s) 294
MalI GATC 1 cut(s) 527
MboI GATC 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 2 cut(s) 416, 443
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 459
MroI TCCGGA 1 cut(s) 469
MspA1I CMGCKG 2 cut(s) 25, 343
MspI CCGG 2 cut(s) 316, 470
MspR9I CCNGG 2 cut(s) 275, 461
MvaI CCWGG 2 cut(s) 275, 461
MwoI GCNNNNNNNGC 7 cut(s) 25, 48, 77, 163, 237, 246, 482
NdeII GATC 1 cut(s) 525
NlaIII CATG 1 cut(s) 547
NlaIV GGNNCC 3 cut(s) 254, 255, 465
PaeR7I CTCGAG 1 cut(s) 119
PceI AGGCCT 1 cut(s) 476
PciSI GCTCTTC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 54, 451
PkrI GCNGC 6 cut(s) 27, 30, 50, 242, 345, 350
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
PpuMI RGGWCCY 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 273, 459
PspFI CCCAGC 2 cut(s) 249, 364
PspGI CCWGG 2 cut(s) 273, 459
PspN4I GGNNCC 3 cut(s) 254, 255, 465
PspPI GGNCC 3 cut(s) 132, 253, 277
PspPPI RGGWCCY 1 cut(s) 253
PstI CTGCAG 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 2 cut(s) 11, 338
RsaNI GTAC 2 cut(s) 10, 337
SacI GAGCTC 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 173
SatI GCNGC 6 cut(s) 26, 29, 49, 241, 344, 349
Sau3AI GATC 1 cut(s) 525
Sau96I GGNCC 3 cut(s) 132, 253, 277
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 2 cut(s) 275, 461
SduI GDGCHC 1 cut(s) 159
SfaNI GCATC 3 cut(s) 28, 330, 412
SfcI CTRYAG 1 cut(s) 26
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 2 cut(s) 132, 253
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 2 cut(s) 416, 443
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 7 cut(s) 5, 42, 48, 51, 207, 241, 343
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 2 cut(s) 273, 459
TaaI ACNGT 1 cut(s) 36
TaiI ACGT 1 cut(s) 297
TaqI TCGA 4 cut(s) 108, 120, 138, 420
TasI AATT 2 cut(s) 416, 443
TauI GCSGC 3 cut(s) 51, 243, 346
TfiI GAWTC 2 cut(s) 54, 451
TscAI CASTG 2 cut(s) 242, 251
TseI GCWGC 3 cut(s) 25, 28, 348
TspDTI ATGAA 5 cut(s) 160, 172, 410, 429, 532
TspRI CASTG 2 cut(s) 242, 251
VpaK11BI GGWCC 2 cut(s) 132, 253
XapI RAATTY 2 cut(s) 416, 443
XhoI CTCGAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.