RLG00000018450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017958
Physical Location & Seq
Forward (+)
29922800 .. 29924351
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018450

Sequence Viewer

Length: 801 bp
ATGTTCTTACTCGGTGGTAAAGCTTCTGCAAGATTTGTTATAAGGAATCATCATGGAATTGCTATAGTTGGTGGTGCGATTCCTATTAGATTTGCTTCAGTTCTTCGAGCAGAGGCTATTGGCTTGAAGATAAGTCTTCCTTGGGCTAAGCTACTCCATATCACTGACCTGGAGGTAGAAGGAGATTCATTGCTGCTAATCGAGATGGTGGAGGGATCTGAAAAAATGAAAACTGACTACGAAACCCACCACCACCATTCCTCGATCCCCAAAGAGACTGCCCTCCAAGCCCTCAACACCATCATCCAGCTCCACTTCGAGAAGACCCTCGAGAAGAAGCGATCCATCGACCTCCAAAAGAAGGAGCTCCAGAAGCTTTTCCAGTTCTTCTTCGTCTTCCTCGCCCTCGTCTTCCTCGCCCAGGCTCAGCCCTCACGCCTCCAATGCCGCCACTGCTGGGTCCCCATCGTCCTCCTCTCCCTCGCCCACCTCATCTTCTACGTCTCCGTCGCCCAGACCCTCCGCTGCATCAATGGCTTTAAGTACCAGAGGCGCTGCCACAAGCTGACGCTCGGCTTGGCCACCGAGAAGCTCAGGGAGATGAAGATGAGGCTCGGTGCCAACGGCGGAGGAGGGCATGAGTTTGATGGGATAGGGGACAATGAGTTCGAGATTCATTATCAGGAGCCACCCGAGAGCTATTTTGGCAAGTTCAAGAGGAACTGGGCTCTGCATTTCGGCTTCTTGATCGTCATCTATGGGTTCATGGTGGCTTCTTCTGTTGTCCTGCTCTGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

30.29

Weight (kDa)

9.16

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 6 - 79 5.5e-08 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18800
fragaria_vesca FvH4_1g23610
malus_domestica MD01G1016600.v1.1
prunus_persica Prupe.6G162500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0119191
rosa_laevigata RLG00000018450
rosa_multiflora Rmu_sc0000184.1_g000011
rosa_roxburghii Rroxscaffold_2G00124460
rosa_rugosa Rorug02G0220800
rosa_samantha Rh2AG278700
rosa_wichuraiana Rw2G022150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 617
AciI CCGC 3 cut(s) 448, 523, 627
AclWI GGATC 3 cut(s) 223, 259, 336
AcoI YGGCCR 1 cut(s) 579
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 3 cut(s) 361, 421, 457
AgsI TTSAA 2 cut(s) 127, 715
AjnI CCWGG 2 cut(s) 168, 420
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 8 cut(s) 23, 151, 310, 367, 376, 565, 592, 699
AluI AGCT 8 cut(s) 23, 151, 310, 367, 376, 565, 592, 699
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 269, 508
AlwI GGATC 3 cut(s) 223, 259, 336
Ama87I CYCGRG 2 cut(s) 329, 692
AoxI GGCC 1 cut(s) 579
ApeKI GCWGC 3 cut(s) 193, 525, 555
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 460
AvaI CYCGRG 2 cut(s) 329, 692
AvaII GGWCC 1 cut(s) 460
BalI TGGCCA 1 cut(s) 581
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 2 cut(s) 369, 730
BbsI GAAGAC 4 cut(s) 128, 329, 388, 403
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 180, 512, 542
BccI CCATC 5 cut(s) 199, 308, 353, 473, 641
BceAI ACGGC 1 cut(s) 640
BciT130I CCWGG 2 cut(s) 170, 422
BcoDI GTCTC 2 cut(s) 269, 508
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 556
BisI GCNGC 4 cut(s) 194, 448, 526, 556
BlpI GCTNAGC 2 cut(s) 147, 426
BlsI GCNGC 4 cut(s) 195, 449, 527, 557
Bme1390I CCNGG 2 cut(s) 170, 422
Bme18I GGWCC 1 cut(s) 460
BmeT110I CYCGRG 2 cut(s) 329, 692
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 4 cut(s) 461, 462, 619, 687
BmrFI CCNGG 2 cut(s) 170, 422
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 537
BmuI ACTGGG 1 cut(s) 733
BpiI GAAGAC 4 cut(s) 128, 329, 388, 403
BpmI CTGGAG 2 cut(s) 191, 353
Bpu10I CCTNAGC 1 cut(s) 593
Bpu1102I GCTNAGC 2 cut(s) 147, 426
BsaBI GATNNNNATC 1 cut(s) 752
BsaJI CCNNGG 2 cut(s) 140, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 421, 457
Bse1I ACTGG 2 cut(s) 382, 728
Bse3DI GCAATG 1 cut(s) 188
Bse8I GATNNNNATC 1 cut(s) 752
BseBI CCWGG 2 cut(s) 170, 422
BseDI CCNNGG 2 cut(s) 140, 420
BseGI GGATG 1 cut(s) 303
BseJI GATNNNNATC 1 cut(s) 752
BseLI CCNNNNNNNGG 3 cut(s) 361, 421, 457
BseMI GCAATG 1 cut(s) 188
BseMII CTCAG 2 cut(s) 440, 607
BseNI ACTGG 2 cut(s) 382, 728
BseRI GAGGAG 2 cut(s) 464, 645
BseXI GCAGC 3 cut(s) 180, 512, 542
BseYI CCCAGC 1 cut(s) 456
BshFI GGCC 1 cut(s) 581
BshNI GGYRCC 1 cut(s) 617
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 2 cut(s) 329, 692
BslFI GGGAC 2 cut(s) 446, 671
BslI CCNNNNNNNGG 3 cut(s) 361, 421, 457
BsmAI GTCTC 2 cut(s) 269, 508
BsmBI CGTCTC 1 cut(s) 508
BsmFI GGGAC 2 cut(s) 446, 671
BsnI GGCC 1 cut(s) 581
BsoBI CYCGRG 2 cut(s) 329, 692
Bsp1286I GDGCHC 2 cut(s) 369, 730
Bsp143I GATC 4 cut(s) 215, 264, 341, 747
Bsp1720I GCTNAGC 2 cut(s) 147, 426
BspACI CCGC 3 cut(s) 448, 523, 627
BspANI GGCC 1 cut(s) 581
BspCNI CTCAG 2 cut(s) 439, 606
BspLI GGNNCC 4 cut(s) 461, 462, 619, 687
BspPI GGATC 3 cut(s) 223, 259, 336
BspT107I GGYRCC 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 188
BsrI ACTGG 2 cut(s) 382, 728
BssECI CCNNGG 2 cut(s) 140, 420
BssMI GATC 4 cut(s) 215, 264, 341, 747
BssT1I CCWWGG 1 cut(s) 140
Bst2UI CCWGG 2 cut(s) 170, 422
BstDEI CTNAG 3 cut(s) 147, 426, 593
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 556
BstHHI GCGC 1 cut(s) 555
BstKTI GATC 4 cut(s) 218, 267, 344, 750
BstMAI GTCTC 2 cut(s) 269, 508
BstMBI GATC 4 cut(s) 215, 264, 341, 747
BstMWI GCNNNNNNNGC 6 cut(s) 287, 373, 444, 453, 534, 705
BstNI CCWGG 2 cut(s) 170, 422
BstSCI CCNGG 2 cut(s) 168, 420
BstSFI CTRYAG 1 cut(s) 63
BstV1I GCAGC 3 cut(s) 180, 512, 542
BstV2I GAAGAC 4 cut(s) 128, 329, 388, 403
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 1 cut(s) 581
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 162, 451
CfoI GCGC 1 cut(s) 555
Cfr13I GGNCC 1 cut(s) 460
CseI GACGC 1 cut(s) 577
Csp6I GTAC 1 cut(s) 544
CviAII CATG 3 cut(s) 53, 638, 766
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 3 cut(s) 147, 426, 593
DpnI GATC 4 cut(s) 217, 266, 343, 749
DpnII GATC 4 cut(s) 215, 264, 341, 747
EaeI YGGCCR 1 cut(s) 579
EciI GGCGGA 1 cut(s) 642
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 369, 730
Eco47I GGWCC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 81
Eco88I CYCGRG 2 cut(s) 329, 692
EcoICRI GAGCTC 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 460
EcoRII CCWGG 2 cut(s) 168, 420
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 2 cut(s) 369, 730
ErhI CCWWGG 1 cut(s) 140
Esp3I CGTCTC 1 cut(s) 508
FaeI CATG 3 cut(s) 56, 641, 769
FaiI YATR 7 cut(s) 41, 54, 65, 159, 639, 759, 767
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FaqI GGGAC 2 cut(s) 446, 671
FatI CATG 3 cut(s) 52, 637, 765
Fnu4HI GCNGC 4 cut(s) 194, 448, 526, 556
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 2 cut(s) 369, 730
Fsp4HI GCNGC 4 cut(s) 194, 448, 526, 556
GlaI GCGC 1 cut(s) 554
GluI GCNGC 4 cut(s) 194, 448, 526, 556
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 2 cut(s) 191, 353
HaeII RGCGCY 1 cut(s) 556
HaeIII GGCC 1 cut(s) 581
HgaI GACGC 1 cut(s) 577
HhaI GCGC 1 cut(s) 555
Hin1II CATG 3 cut(s) 56, 641, 769
Hin6I GCGC 1 cut(s) 553
HinP1I GCGC 1 cut(s) 553
HindIII AAGCTT 2 cut(s) 21, 374
HinfI GANTC 4 cut(s) 46, 79, 185, 673
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 8 cut(s) 202, 319, 331, 370, 670, 683, 715, 745
Hpy99I CGWCG 1 cut(s) 512
HpyAV CCTTC 2 cut(s) 173, 355
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 3 cut(s) 29, 528, 733
HpyF10VI GCNNNNNNNGC 6 cut(s) 287, 373, 444, 453, 534, 705
HpyF3I CTNAG 3 cut(s) 147, 426, 593
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 3 cut(s) 56, 641, 769
HspAI GCGC 1 cut(s) 553
KflI GGGWCCC 1 cut(s) 460
Kzo9I GATC 4 cut(s) 215, 264, 341, 747
LmnI GCTCC 4 cut(s) 315, 364, 372, 685
Lsp1109I GCAGC 3 cut(s) 180, 512, 542
LweI GCATC 1 cut(s) 537
MaeII ACGT 1 cut(s) 501
MalI GATC 4 cut(s) 217, 266, 343, 749
MboI GATC 4 cut(s) 215, 264, 341, 747
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 2 cut(s) 369, 730
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 1 cut(s) 57
MluNI TGGCCA 1 cut(s) 581
Mox20I TGGCCA 1 cut(s) 581
MroXI GAANNNNTTC 1 cut(s) 377
MscI TGGCCA 1 cut(s) 581
MseI TTAA 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 525
MspR9I CCNGG 2 cut(s) 170, 422
MvaI CCWGG 2 cut(s) 170, 422
MwoI GCNNNNNNNGC 6 cut(s) 287, 373, 444, 453, 534, 705
NdeII GATC 4 cut(s) 215, 264, 341, 747
NlaIII CATG 3 cut(s) 56, 641, 769
NlaIV GGNNCC 4 cut(s) 461, 462, 619, 687
NmeAIII GCCGAG 1 cut(s) 552
PaeR7I CTCGAG 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 4 cut(s) 46, 79, 185, 673
PkrI GCNGC 4 cut(s) 195, 449, 527, 557
PpuMI RGGWCCY 1 cut(s) 460
PsiI TTATAA 1 cut(s) 41
Psp124BI GAGCTC 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 460
Psp6I CCWGG 2 cut(s) 168, 420
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 2 cut(s) 168, 420
PspN4I GGNNCC 4 cut(s) 461, 462, 619, 687
PspPI GGNCC 1 cut(s) 460
PspPPI RGGWCCY 1 cut(s) 460
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
SacI GAGCTC 1 cut(s) 369
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 4 cut(s) 194, 448, 526, 556
Sau3AI GATC 4 cut(s) 215, 264, 341, 747
Sau96I GGNCC 1 cut(s) 460
ScrFI CCNGG 2 cut(s) 170, 422
SduI GDGCHC 2 cut(s) 369, 730
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 1 cut(s) 63
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 460
SlaI CTCGAG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 1 cut(s) 57
SsiI CCGC 3 cut(s) 448, 523, 627
SstI GAGCTC 1 cut(s) 369
StyD4I CCNGG 2 cut(s) 168, 420
StyI CCWWGG 1 cut(s) 140
TaiI ACGT 1 cut(s) 504
TaqI TCGA 7 cut(s) 106, 201, 263, 318, 330, 348, 669
TasI AATT 1 cut(s) 57
TauI GCSGC 1 cut(s) 450
TfiI GAWTC 4 cut(s) 46, 79, 185, 673
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 2 cut(s) 169, 458
TseI GCWGC 3 cut(s) 193, 525, 555
TspDTI ATGAA 5 cut(s) 177, 242, 617, 665, 754
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 2 cut(s) 169, 458
VpaK11BI GGWCC 1 cut(s) 460
XagI CCTNNNNNAGG 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.