MD01G1035100.v1.1

lanC-like protein GCL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014537
Physical Location & Seq
Reverse (-)
11928882 .. 11934218
5337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1035100.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGTCGTTTGGAGTGGAGTTTGCGTTGCCTCCGGAGGACCACGAAGATGCCAACAAGGAACGGCTGGATTTGGTTGATCCAGCGGCTAAGAACTTGTCACTTCCGAGCGGGACTTTTCTCAGAGCGGCGGTGGCTCTCAAGGATCAGGTGGTGCAGGATACATGGGAAGGAGGACGTGGTTCGGGTACGCCTACGGGTATGGTTTTCGATCCGACAGTGTATACGGGTCTCCTTGGTACGGCTTTTACGTGCTTGAGGTCGTATGAGGCCACCGGCAACCGGCAGGACTTGCTGCTCTGCGCTGATATCGTCGACAAGTGCGGGGCCGTCGCACCTGCGTCCAGCAGGCACGTGACTTTTTTATGTGGTAGGGGAGGAGTCTACGCCCTAGGAGCTGTGGTGGCTAGTTTGATGGGGGACCATGAAAGATGTGGCCTCTATTTTAACCTGTTTCTTGAGGTGGCACAAGAGAAGGCCTTGTCTGTTGGGCCAGAGGAAGGTGGTTTTGGAATGTCGTACGATCTTCTCTACGGGAGAGCTGGGATTTTATGGGCAGCTTTGTTCATAAACAAGTATCTTGGACAGGACAAGTTGCCAAGTGATCTTCTAATGCCTGTTGTGGATGCTGTGTTAGCTGGTGGTAGGGCGGGCGCATCTCATGACCTGTCCTGCCCACTTATGTACAGATGGCACGGGACTAGGTACTTGGGGGCAGCCAATGGCGTGGCCGGAATTTTGCAAGTGCTGCTTCACTTCCCACTTTGTAATGAGGATGCAGAAGATGTGAAGGCAACTCTTAGATATATGATGGCTAATAGGTTTCCTCATAGTGGAAACTACCCCTCAAGTGAAGGAAACCCTAGAGACAAGTTTGTGCAGTGGTCTCATGGTGCAGCTGGCATGGCCATAACCTTGTGCAAGGCATCTCAGGTGTTTCCAGGTGATCGGGAATTTCGTGACGCTGCAATAGAAGCAGGAGAAGTTGTTTGGAAGAACGGTTTACTGAAGAAGGTAGGACTTGCTGACGGTGTGGCTGGGAATGCTTATGCCTTCCTTTCTCTGTATCGTCTGACTGGAGAGAGCATATACGAAGAGAGAGCAAAGGCATTTGCAAGCTTCTTGTATCATAGTGCTGGAGAGTTTTCGATTGCAGGACGCACTCATGGGGTTGAATATGTCTACTCTCTTTTTCAAGGTCTTGCTGGAACAGCTTGCCTCTGGTTTGATCTTCATGTGCCCGAAAATTCTAGGTTTCCGGGTTATGAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0019898 GO:0023052 GO:0044425 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LANC_like

Protein Analysis

424

Amino Acids

45.69

Weight (kDa)

5.41

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LANC_like PF05147 69 - 423 2.1e-89 Lanthionine synthetase C-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65280 AT5G65280
fragaria_vesca FvH4_1g20300
malus_domestica MD01G1035100.v1.1 MD15G1320800.v1.1
prunus_persica Prupe.6G192300_v2.0.a1
pyrus_communis pycom15g28180
rosa_chinensis RchiOBHm_Chr2g0112441
rosa_laevigata RLG00000017981
rosa_multiflora Rmu_sc0012231.1_g000002
rosa_roxburghii Rroxscaffold_2G00131440
rosa_rugosa Rorug02G0180600
rosa_samantha Rh2AG234000 Rh2BG247300 Rh2CG238500 Rh2DG241600
rosa_wichuraiana Rw2G018120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 1 cut(s) 343
AccBSI CCGCTC 2 cut(s) 108, 125
AccI GTMKAC 4 cut(s) 221, 312, 381, 1179
AccIII TCCGGA 1 cut(s) 31
AciI CCGC 6 cut(s) 83, 108, 125, 128, 321, 647
AclWI GGATC 3 cut(s) 71, 150, 203
AcoI YGGCCR 2 cut(s) 726, 903
AcsI RAATTY 3 cut(s) 732, 950, 1243
AcuI CTGAAG 1 cut(s) 1025
AcvI CACGTG 1 cut(s) 352
AfaI GTAC 5 cut(s) 187, 238, 518, 683, 704
AfiI CCNNNNNNNGG 4 cut(s) 238, 279, 497, 830
AgsI TTSAA 2 cut(s) 1172, 1193
AjiI CACGTC 1 cut(s) 176
AjnI CCWGG 1 cut(s) 937
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AluBI AGCT 7 cut(s) 395, 539, 557, 635, 896, 1116, 1211
AluI AGCT 7 cut(s) 395, 539, 557, 635, 896, 1116, 1211
Alw26I GTCTC 3 cut(s) 233, 858, 888
AlwI GGATC 3 cut(s) 71, 150, 203
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 7 cut(s) 267, 324, 433, 474, 488, 726, 903
ApeKI GCWGC 6 cut(s) 292, 554, 713, 745, 893, 962
ApoI RAATTY 3 cut(s) 732, 950, 1243
AspA2I CCTAGG 1 cut(s) 388
AspLEI GCGC 2 cut(s) 302, 653
AspS9I GGNCC 4 cut(s) 37, 324, 418, 488
AsuC2I CCSGG 1 cut(s) 1257
AsuHPI GGTGA 1 cut(s) 953
AvaII GGWCC 2 cut(s) 37, 418
AvrII CCTAGG 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 1239
BalI TGGCCA 1 cut(s) 905
BbrPI CACGTG 1 cut(s) 352
BbvI GCAGC 6 cut(s) 279, 566, 725, 732, 905, 949
BccI CCATC 3 cut(s) 406, 681, 802
BceAI ACGGC 3 cut(s) 77, 255, 311
BciT130I CCWGG 1 cut(s) 939
BciVI GTATCC 1 cut(s) 151
BcnI CCSGG 1 cut(s) 1257
BcoDI GTCTC 3 cut(s) 233, 858, 888
BfaI CTAG 5 cut(s) 389, 405, 699, 861, 1248
BfuAI ACCTGC 1 cut(s) 343
BfuI GTATCC 1 cut(s) 151
BisI GCNGC 8 cut(s) 84, 126, 293, 555, 714, 746, 894, 963
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 8 cut(s) 85, 127, 294, 556, 715, 747, 895, 964
Bme1390I CCNGG 2 cut(s) 939, 1257
Bme18I GGWCC 2 cut(s) 37, 418
BmgBI CACGTC 1 cut(s) 176
BmgT120I GGNCC 4 cut(s) 37, 324, 418, 488
BmiI GGNNCC 2 cut(s) 325, 419
BmrFI CCNGG 2 cut(s) 939, 1257
BmsI GCATC 5 cut(s) 37, 613, 662, 763, 932
BpmI CTGGAG 2 cut(s) 1095, 1155
BpuEI CTTGAG 4 cut(s) 122, 274, 476, 829
BpuMI CCSGG 1 cut(s) 1257
BsaAI YACGTR 2 cut(s) 249, 352
BsaI GGTCTC 2 cut(s) 233, 888
BsaJI CCNNGG 2 cut(s) 232, 388
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 384, 414, 969, 999
Bsc4I CCNNNNNNNGG 4 cut(s) 238, 279, 497, 830
Bse118I RCCGGY 2 cut(s) 272, 279
Bse1I ACTGG 1 cut(s) 1078
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 1 cut(s) 939
BseDI CCNNGG 2 cut(s) 232, 388
BseGI GGATG 2 cut(s) 628, 778
BseLI CCNNNNNNNGG 4 cut(s) 238, 279, 497, 830
BseMII CTCAG 2 cut(s) 133, 941
BseNI ACTGG 1 cut(s) 1078
BseRI GAGGAG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 1239
BseXI GCAGC 6 cut(s) 279, 566, 725, 732, 905, 949
BseYI CCCAGC 2 cut(s) 539, 1034
BsgI GTGCAG 3 cut(s) 173, 896, 912
BshFI GGCC 7 cut(s) 269, 326, 435, 476, 490, 728, 905
BsiSI CCGG 5 cut(s) 32, 273, 280, 729, 1256
BsiWI CGTACG 1 cut(s) 516
BslFI GGGAC 3 cut(s) 124, 431, 709
BslI CCNNNNNNNGG 4 cut(s) 238, 279, 497, 830
BsmAI GTCTC 3 cut(s) 233, 858, 888
BsmFI GGGAC 3 cut(s) 124, 431, 709
BsmI GAATGC 1 cut(s) 1045
BsnI GGCC 7 cut(s) 269, 326, 435, 476, 490, 728, 905
Bso31I GGTCTC 2 cut(s) 233, 888
Bsp1286I GDGCHC 1 cut(s) 1239
Bsp13I TCCGGA 1 cut(s) 31
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
BspACI CCGC 6 cut(s) 83, 108, 125, 128, 321, 647
BspANI GGCC 7 cut(s) 269, 326, 435, 476, 490, 728, 905
BspCNI CTCAG 2 cut(s) 132, 940
BspEI TCCGGA 1 cut(s) 31
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 2 cut(s) 325, 419
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 3 cut(s) 71, 150, 203
BspTNI GGTCTC 2 cut(s) 233, 888
BsrBI CCGCTC 2 cut(s) 108, 125
BsrFI RCCGGY 2 cut(s) 272, 279
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 1 cut(s) 1078
BssAI RCCGGY 2 cut(s) 272, 279
BssECI CCNNGG 2 cut(s) 232, 388
BssMI GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
BssNAI GTATAC 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 232, 388
Bst1107I GTATAC 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 939
Bst4CI ACNGT 4 cut(s) 217, 998, 1028, 1269
Bst6I CTCTTC 1 cut(s) 1086
BstAPI GCANNNNNTGC 2 cut(s) 289, 745
BstAUI TGTACA 1 cut(s) 681
BstBAI YACGTR 2 cut(s) 249, 352
BstC8I GCNNGC 5 cut(s) 347, 649, 898, 1114, 1213
BstDEI CTNAG 4 cut(s) 87, 119, 797, 927
BstF5I GGATG 2 cut(s) 628, 778
BstHHI GCGC 2 cut(s) 302, 653
BstKTI GATC 7 cut(s) 79, 145, 211, 523, 604, 946, 1228
BstMAI GTCTC 3 cut(s) 233, 858, 888
BstMBI GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
BstNI CCWGG 1 cut(s) 939
BstSCI CCNGG 2 cut(s) 937, 1255
BstSLI GKGCMC 1 cut(s) 1239
BstV1I GCAGC 6 cut(s) 279, 566, 725, 732, 905, 949
BstXI CCANNNNNNTGG 1 cut(s) 724
BstZ17I GTATAC 1 cut(s) 222
BsuI GTATCC 1 cut(s) 151
BsuRI GGCC 7 cut(s) 269, 326, 435, 476, 490, 728, 905
BtrI CACGTC 1 cut(s) 176
BtsCI GGATG 2 cut(s) 628, 778
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 2 cut(s) 222, 884
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 5 cut(s) 347, 649, 898, 1114, 1213
CciI TCATGA 1 cut(s) 658
CfoI GCGC 2 cut(s) 302, 653
Cfr10I RCCGGY 2 cut(s) 272, 279
Cfr13I GGNCC 4 cut(s) 37, 324, 418, 488
CseI GACGC 3 cut(s) 327, 968, 1164
Csp6I GTAC 5 cut(s) 186, 237, 517, 682, 703
CviAII CATG 7 cut(s) 162, 422, 659, 887, 901, 1163, 1232
CviQI GTAC 5 cut(s) 186, 237, 517, 682, 703
DdeI CTNAG 4 cut(s) 87, 119, 797, 927
DpnI GATC 7 cut(s) 78, 144, 210, 522, 603, 945, 1227
DpnII GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
EaeI YGGCCR 2 cut(s) 726, 903
Eam1104I CTCTTC 1 cut(s) 1086
EarI CTCTTC 1 cut(s) 1086
Eco130I CCWWGG 2 cut(s) 232, 388
Eco147I AGGCCT 1 cut(s) 476
Eco31I GGTCTC 2 cut(s) 233, 888
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 37, 418
Eco57I CTGAAG 1 cut(s) 1025
Eco72I CACGTG 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 937
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 232, 388
ErhI CCWWGG 2 cut(s) 232, 388
FaeI CATG 7 cut(s) 165, 425, 662, 890, 904, 1166, 1235
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 3 cut(s) 124, 431, 709
FatI CATG 7 cut(s) 161, 421, 658, 886, 900, 1162, 1231
FauI CCCGC 3 cut(s) 101, 314, 640
FblI GTMKAC 4 cut(s) 221, 312, 381, 1179
Fnu4HI GCNGC 8 cut(s) 84, 126, 293, 555, 714, 746, 894, 963
FokI GGATG 2 cut(s) 635, 785
Fsp4HI GCNGC 8 cut(s) 84, 126, 293, 555, 714, 746, 894, 963
FspBI CTAG 5 cut(s) 389, 405, 699, 861, 1248
GlaI GCGC 2 cut(s) 301, 652
GluI GCNGC 8 cut(s) 84, 126, 293, 555, 714, 746, 894, 963
GsaI CCCAGC 2 cut(s) 543, 1038
GsuI CTGGAG 2 cut(s) 1095, 1155
HaeIII GGCC 7 cut(s) 269, 326, 435, 476, 490, 728, 905
HapII CCGG 5 cut(s) 32, 273, 280, 729, 1256
HgaI GACGC 3 cut(s) 327, 968, 1164
HhaI GCGC 2 cut(s) 302, 653
Hin1II CATG 7 cut(s) 165, 425, 662, 890, 904, 1166, 1235
Hin6I GCGC 2 cut(s) 300, 651
HinP1I GCGC 2 cut(s) 300, 651
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 1114
HinfI GANTC 1 cut(s) 378
HpaII CCGG 5 cut(s) 32, 273, 280, 729, 1256
HphI GGTGA 1 cut(s) 953
Hpy166II GTNNAC 5 cut(s) 222, 313, 382, 1001, 1180
Hpy188I TCNGA 4 cut(s) 105, 122, 213, 1071
Hpy188III TCNNGA 5 cut(s) 32, 455, 659, 947, 956
Hpy8I GTNNAC 5 cut(s) 222, 313, 382, 1001, 1180
Hpy99I CGWCG 2 cut(s) 314, 332
HpyAV CCTTC 7 cut(s) 161, 466, 491, 781, 845, 1003, 1060
HpyCH4III ACNGT 4 cut(s) 217, 998, 1028, 1269
HpyCH4IV ACGT 3 cut(s) 175, 248, 351
HpyCH4V TGCA 9 cut(s) 154, 739, 776, 877, 893, 918, 965, 1112, 1151
HpyF3I CTNAG 4 cut(s) 87, 119, 797, 927
HpySE526I ACGT 3 cut(s) 175, 248, 351
Hsp92II CATG 7 cut(s) 165, 425, 662, 890, 904, 1166, 1235
HspAI GCGC 2 cut(s) 300, 651
Kpn2I TCCGGA 1 cut(s) 31
Kzo9I GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
LmnI GCTCC 1 cut(s) 392
Lsp1109I GCAGC 6 cut(s) 279, 566, 725, 732, 905, 949
LweI GCATC 5 cut(s) 37, 613, 662, 763, 932
MaeI CTAG 5 cut(s) 389, 405, 699, 861, 1248
MaeII ACGT 3 cut(s) 175, 248, 351
MaeIII GTNAC 3 cut(s) 96, 352, 956
MalI GATC 7 cut(s) 78, 144, 210, 522, 603, 945, 1227
MbiI CCGCTC 2 cut(s) 108, 125
MboI GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
MboII GAAGA 8 cut(s) 56, 515, 596, 791, 1003, 1018, 1103, 1220
MhlI GDGCHC 1 cut(s) 1239
MlsI TGGCCA 1 cut(s) 905
MluCI AATT 3 cut(s) 732, 950, 1243
MluNI TGGCCA 1 cut(s) 905
MlyI GAGTC 1 cut(s) 387
MmeI TCCRAC 1 cut(s) 236
Mox20I TGGCCA 1 cut(s) 905
MroI TCCGGA 1 cut(s) 31
MscI TGGCCA 1 cut(s) 905
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 905
MspA1I CMGCKG 2 cut(s) 83, 896
MspI CCGG 5 cut(s) 32, 273, 280, 729, 1256
MspR9I CCNGG 2 cut(s) 939, 1257
Mva1269I GAATGC 1 cut(s) 1045
MvaI CCWGG 1 cut(s) 939
NciI CCSGG 1 cut(s) 1257
NdeII GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
NlaIII CATG 7 cut(s) 165, 425, 662, 890, 904, 1166, 1235
NlaIV GGNNCC 2 cut(s) 325, 419
NmuCI GTSAC 3 cut(s) 96, 352, 956
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 343
PceI AGGCCT 1 cut(s) 476
PcsI WCGNNNNNNNCGW 2 cut(s) 245, 952
PctI GAATGC 1 cut(s) 1045
Pfl23II CGTACG 1 cut(s) 516
PkrI GCNGC 8 cut(s) 85, 127, 294, 556, 715, 747, 895, 964
PleI GAGTC 1 cut(s) 386
PmaCI CACGTG 1 cut(s) 352
PmlI CACGTG 1 cut(s) 352
PpsI GAGTC 1 cut(s) 386
Ppu21I YACGTR 2 cut(s) 249, 352
Psp6I CCWGG 1 cut(s) 937
PspCI CACGTG 1 cut(s) 352
PspFI CCCAGC 2 cut(s) 539, 1034
PspGI CCWGG 1 cut(s) 937
PspLI CGTACG 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 325, 419
PspPI GGNCC 4 cut(s) 37, 324, 418, 488
PvuII CAGCTG 1 cut(s) 896
RsaI GTAC 5 cut(s) 187, 238, 518, 683, 704
RsaNI GTAC 5 cut(s) 186, 237, 517, 682, 703
RseI CAYNNNNRTG 1 cut(s) 45
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 8 cut(s) 84, 126, 293, 555, 714, 746, 894, 963
Sau3AI GATC 7 cut(s) 76, 142, 208, 520, 601, 943, 1225
Sau96I GGNCC 4 cut(s) 37, 324, 418, 488
SchI GAGTC 1 cut(s) 387
ScrFI CCNGG 2 cut(s) 939, 1257
SduI GDGCHC 1 cut(s) 1239
SfaNI GCATC 5 cut(s) 37, 613, 662, 763, 932
SinI GGWCC 2 cut(s) 37, 418
SmiMI CAYNNNNRTG 1 cut(s) 45
SmlI CTYRAG 4 cut(s) 137, 253, 455, 844
SmoI CTYRAG 4 cut(s) 137, 253, 455, 844
Sse9I AATT 3 cut(s) 732, 950, 1243
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 6 cut(s) 83, 108, 125, 128, 321, 647
SspMI CTAG 5 cut(s) 389, 405, 699, 861, 1248
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 2 cut(s) 937, 1255
StyI CCWWGG 2 cut(s) 232, 388
TaaI ACNGT 4 cut(s) 217, 998, 1028, 1269
TaiI ACGT 3 cut(s) 178, 251, 354
TaqI TCGA 3 cut(s) 207, 312, 1145
TasI AATT 3 cut(s) 732, 950, 1243
TatI WGTACW 1 cut(s) 681
TauI GCSGC 2 cut(s) 86, 128
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 2 cut(s) 222, 884
TseFI GTSAC 3 cut(s) 96, 352, 956
TseI GCWGC 6 cut(s) 292, 554, 713, 745, 893, 962
Tsp45I GTSAC 3 cut(s) 96, 352, 956
TspDTI ATGAA 3 cut(s) 438, 553, 1220
TspRI CASTG 2 cut(s) 222, 884
VpaK11BI GGWCC 2 cut(s) 37, 418
XapI RAATTY 3 cut(s) 732, 950, 1243
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XmaJI CCTAGG 1 cut(s) 388
XmiI GTMKAC 4 cut(s) 221, 312, 381, 1179
XspI CTAG 5 cut(s) 389, 405, 699, 861, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.