MD15G1320800.v1.1

lanC-like protein GCL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014537
Physical Location & Seq
Forward (+)
33338465 .. 33344777
6313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1320800.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGTCGTTTGGCGTGGAGTTTGCCCCTTCTCCGGAGGAACACGATGATGCCAACAACGAACGGCTGGATTTGGTCGATCCAGCGGCTCCGACCTTGTCACCTTCGAGCGGGACTTTTCTCAGAGCGGCGGTGACTCTCAAGGATCAGGTGGTGCAGGCTACGTGGGAAGGAGGGCATGATTCGGGTACGCCTACGGGTATGGTGTTGGATCCGACAGTTTATACGGGTCTTCTTGGTACGGCTTTTACGTGCTTGAGGTCGTACGAGGCCGCCGGTAACCGGCAGGACTTGGTGCTGTGCGCTGAGATCGTCGACAAGTGCCGGGCTGTCGCAAGTGCGTCAACCAGGCACGTGACTTTTTTATGTGGTAGAGGAGGAGTGTACGCCCTAGGAGCTGTGGTGGCCAATTTGATTGGGGACCATGAAAGACGTAACCTGTATTTGAATCTGTTTCTTGAGGTGGCACAAGAGAAGGCCCTGTCTGTTGGGCCGGAGGAAGGTGGTTTTGGAATGTCGTACGATCTTCTCTACGGGAGAGCTGGGTTTTTATGGGCAGCTTTGTTCATAAACAAGTATCTTGGACAAGAAAAGTTGCCAAATAATATTCTAATGCCTGTTGTGGATGCTGTGTTAGCTGGTGGTAGGGCAGGCTCATCTCATAACCCGTCCTGCCCGCTCATGTTCAGGTGGCACGGGACTAGGTATTTGGGGGCAGCCAATGGCGTGGCTGGAATTTTGCAAGTGCTGCTTCACTTCCCACTTTGCAAAGAGGATGCTGACGATGTGAAGGCAACGCTTAGATATATGATGGCTAATAGGTTCCCTCACAGTGGAAACTACCCGTCAAGTGAAGGAAACCCTAGAGACAAGTTTGTGCAGTGGTCTCATGGTGCAGCTGGCATGGCCATAACCTTGTGCAAGGCATCTCAGGTGTTTCCAGGTGATCGGGAATTTCGCGATGCTGCAATAGAAGCAGGAGAAGTTGTGTGGAAGAACGGTTTACTGAAGAAGGTAGGACTTGCTGATGGTGTGGCTGGGAATGCCTATGCCTTCCTTTCTCTGTATCGTCTGACTGGAGAGAGCATATACGAAGAGAGAGCAAGGGCATTTGCAAGCTTCTTGTATCATAATGCCGGAGAGTTTGCGACTGCGGGACACACACATGGGGCCGAATATGCCTACTCTCTCTTTCAAGGTCTTGCCGGAACAGCTTGCCTCTGGTTTGATCTTTATATACCCGAAAATTCTAGATTTCCGGGTTATGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0019898 GO:0023052 GO:0044425 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LANC_like

Protein Analysis

424

Amino Acids

45.77

Weight (kDa)

5.57

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LANC_like PF05147 68 - 423 2.8e-88 Lanthionine synthetase C-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65280 AT5G65280
fragaria_vesca FvH4_1g20300
malus_domestica MD01G1035100.v1.1 MD15G1320800.v1.1
prunus_persica Prupe.6G192300_v2.0.a1
pyrus_communis pycom15g28180
rosa_chinensis RchiOBHm_Chr2g0112441
rosa_laevigata RLG00000017981
rosa_multiflora Rmu_sc0012231.1_g000002
rosa_roxburghii Rroxscaffold_2G00131440
rosa_rugosa Rorug02G0180600
rosa_samantha Rh2AG234000 Rh2BG247300 Rh2CG238500 Rh2DG241600
rosa_wichuraiana Rw2G018120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 108, 125, 676
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 957
AccIII TCCGGA 1 cut(s) 31
AciI CCGC 7 cut(s) 83, 108, 125, 128, 270, 674, 1151
AclWI GGATC 4 cut(s) 71, 150, 203, 216
AcoI YGGCCR 2 cut(s) 402, 903
AcsI RAATTY 3 cut(s) 732, 950, 1243
AcuI CTGAAG 1 cut(s) 1025
AcvI CACGTG 1 cut(s) 352
AfaI GTAC 5 cut(s) 187, 238, 263, 383, 518
AfiI CCNNNNNNNGG 5 cut(s) 31, 107, 279, 497, 830
AgsI TTSAA 2 cut(s) 445, 1193
AjnI CCWGG 2 cut(s) 344, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AluBI AGCT 7 cut(s) 395, 539, 557, 635, 896, 1116, 1211
AluI AGCT 7 cut(s) 395, 539, 557, 635, 896, 1116, 1211
Alw26I GTCTC 2 cut(s) 858, 888
AlwI GGATC 4 cut(s) 71, 150, 203, 216
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 6 cut(s) 267, 402, 474, 488, 903, 1167
ApeKI GCWGC 5 cut(s) 554, 713, 745, 893, 962
ApoI RAATTY 3 cut(s) 732, 950, 1243
ArsI GACNNNNNNTTYG 2 cut(s) 688, 720
AspA2I CCTAGG 1 cut(s) 388
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 4 cut(s) 418, 475, 488, 1167
AsuC2I CCSGG 2 cut(s) 323, 1257
AsuHPI GGTGA 3 cut(s) 90, 142, 953
AvaII GGWCC 1 cut(s) 418
AvrII CCTAGG 1 cut(s) 388
BalI TGGCCA 2 cut(s) 404, 905
BamHI GGATCC 1 cut(s) 208
BbrPI CACGTG 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 221
BbvI GCAGC 5 cut(s) 566, 725, 732, 905, 949
BccI CCATC 2 cut(s) 802, 1019
BceAI ACGGC 2 cut(s) 77, 255
BciT130I CCWGG 2 cut(s) 346, 939
BcnI CCSGG 2 cut(s) 323, 1257
BcoDI GTCTC 2 cut(s) 858, 888
BfaI CTAG 4 cut(s) 389, 699, 861, 1248
BfmI CTRYAG 1 cut(s) 1268
BisI GCNGC 8 cut(s) 84, 126, 270, 555, 714, 746, 894, 963
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 8 cut(s) 85, 127, 271, 556, 715, 747, 895, 964
Bme1390I CCNGG 4 cut(s) 323, 346, 939, 1257
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 4 cut(s) 418, 475, 488, 1167
BmiI GGNNCC 5 cut(s) 87, 210, 419, 821, 1168
BmrFI CCNGG 4 cut(s) 323, 346, 939, 1257
BmsI GCATC 5 cut(s) 37, 613, 763, 932, 949
BpiI GAAGAC 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 1095
BpuEI CTTGAG 3 cut(s) 122, 274, 476
BpuMI CCSGG 2 cut(s) 323, 1257
BsaAI YACGTR 3 cut(s) 162, 249, 352
BsaI GGTCTC 1 cut(s) 888
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 969, 999
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 107, 279, 497, 830
Bse118I RCCGGY 2 cut(s) 272, 279
Bse1I ACTGG 1 cut(s) 1078
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 2 cut(s) 346, 939
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 2 cut(s) 628, 778
BseLI CCNNNNNNNGG 5 cut(s) 31, 107, 279, 497, 830
BseMII CTCAG 3 cut(s) 133, 294, 941
BseNI ACTGG 1 cut(s) 1078
BseRI GAGGAG 2 cut(s) 387, 390
BseXI GCAGC 5 cut(s) 566, 725, 732, 905, 949
BseYI CCCAGC 2 cut(s) 539, 1034
BsgI GTGCAG 3 cut(s) 173, 896, 912
Bsh1236I CGCG 1 cut(s) 957
BshFI GGCC 6 cut(s) 269, 404, 476, 490, 905, 1169
BsiSI CCGG 8 cut(s) 32, 273, 280, 322, 491, 1134, 1203, 1256
BsiWI CGTACG 2 cut(s) 261, 516
BslFI GGGAC 4 cut(s) 124, 431, 709, 1167
BslI CCNNNNNNNGG 5 cut(s) 31, 107, 279, 497, 830
BsmAI GTCTC 2 cut(s) 858, 888
BsmFI GGGAC 4 cut(s) 124, 431, 709, 1167
BsmI GAATGC 1 cut(s) 1045
BsnI GGCC 6 cut(s) 269, 404, 476, 490, 905, 1169
Bso31I GGTCTC 1 cut(s) 888
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
Bsp68I TCGCGA 1 cut(s) 957
BspACI CCGC 7 cut(s) 83, 108, 125, 128, 270, 674, 1151
BspANI GGCC 6 cut(s) 269, 404, 476, 490, 905, 1169
BspCNI CTCAG 3 cut(s) 132, 295, 940
BspEI TCCGGA 1 cut(s) 31
BspFNI CGCG 1 cut(s) 957
BspLI GGNNCC 5 cut(s) 87, 210, 419, 821, 1168
BspPI GGATC 4 cut(s) 71, 150, 203, 216
BspTNI GGTCTC 1 cut(s) 888
BsrBI CCGCTC 3 cut(s) 108, 125, 676
BsrFI RCCGGY 2 cut(s) 272, 279
BsrI ACTGG 1 cut(s) 1078
BssAI RCCGGY 2 cut(s) 272, 279
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 2 cut(s) 346, 939
Bst4CI ACNGT 4 cut(s) 217, 830, 998, 1269
Bst6I CTCTTC 1 cut(s) 1086
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBAI YACGTR 3 cut(s) 162, 249, 352
BstC8I GCNNGC 6 cut(s) 156, 649, 674, 898, 1114, 1213
BstDEI CTNAG 4 cut(s) 119, 303, 797, 927
BstEII GGTNACC 1 cut(s) 275
BstF5I GGATG 2 cut(s) 628, 778
BstFNI CGCG 1 cut(s) 957
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 7 cut(s) 79, 145, 211, 309, 523, 946, 1228
BstMAI GTCTC 2 cut(s) 858, 888
BstMBI GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
BstMWI GCNNNNNNNGC 9 cut(s) 392, 401, 632, 745, 902, 971, 1040, 1175, 1208
BstNI CCWGG 2 cut(s) 346, 939
BstPI GGTNACC 1 cut(s) 275
BstSCI CCNGG 4 cut(s) 321, 344, 937, 1255
BstSFI CTRYAG 1 cut(s) 1268
BstUI CGCG 1 cut(s) 957
BstV1I GCAGC 5 cut(s) 566, 725, 732, 905, 949
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 6 cut(s) 269, 404, 476, 490, 905, 1169
BtgZI GCGATG 1 cut(s) 972
BtsCI GGATG 2 cut(s) 628, 778
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 2 cut(s) 835, 884
BtuMI TCGCGA 1 cut(s) 957
Cac8I GCNNGC 6 cut(s) 156, 649, 674, 898, 1114, 1213
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 2 cut(s) 272, 279
Cfr13I GGNCC 4 cut(s) 418, 475, 488, 1167
CseI GACGC 1 cut(s) 327
Csp6I GTAC 5 cut(s) 186, 237, 262, 382, 517
CviAII CATG 6 cut(s) 176, 422, 679, 887, 901, 1163
CviQI GTAC 5 cut(s) 186, 237, 262, 382, 517
DdeI CTNAG 4 cut(s) 119, 303, 797, 927
DpnI GATC 7 cut(s) 78, 144, 210, 308, 522, 945, 1227
DpnII GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
EaeI YGGCCR 2 cut(s) 402, 903
Eam1104I CTCTTC 1 cut(s) 1086
EarI CTCTTC 1 cut(s) 1086
Eco130I CCWWGG 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 888
Eco47I GGWCC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 1025
Eco72I CACGTG 1 cut(s) 352
Eco91I GGTNACC 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 475
EcoO65I GGTNACC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 344, 937
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 6 cut(s) 179, 425, 682, 890, 904, 1166
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 4 cut(s) 124, 431, 709, 1167
FatI CATG 6 cut(s) 175, 421, 678, 886, 900, 1162
FauI CCCGC 3 cut(s) 101, 681, 1144
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 8 cut(s) 84, 126, 270, 555, 714, 746, 894, 963
FokI GGATG 2 cut(s) 635, 785
Fsp4HI GCNGC 8 cut(s) 84, 126, 270, 555, 714, 746, 894, 963
FspBI CTAG 4 cut(s) 389, 699, 861, 1248
GlaI GCGC 1 cut(s) 301
GluI GCNGC 8 cut(s) 84, 126, 270, 555, 714, 746, 894, 963
GsaI CCCAGC 2 cut(s) 543, 1038
GsuI CTGGAG 1 cut(s) 1095
HaeIII GGCC 6 cut(s) 269, 404, 476, 490, 905, 1169
HapII CCGG 8 cut(s) 32, 273, 280, 322, 491, 1134, 1203, 1256
HgaI GACGC 1 cut(s) 327
HhaI GCGC 1 cut(s) 302
Hin1II CATG 6 cut(s) 179, 425, 682, 890, 904, 1166
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 2 cut(s) 313, 342
HindII GTYRAC 2 cut(s) 313, 342
HindIII AAGCTT 1 cut(s) 1114
HinfI GANTC 3 cut(s) 133, 179, 445
HpaII CCGG 8 cut(s) 32, 273, 280, 322, 491, 1134, 1203, 1256
HphI GGTGA 3 cut(s) 90, 142, 953
Hpy166II GTNNAC 4 cut(s) 313, 342, 382, 1001
Hpy188I TCNGA 4 cut(s) 90, 122, 213, 1071
Hpy188III TCNNGA 5 cut(s) 32, 455, 947, 956, 1248
Hpy8I GTNNAC 4 cut(s) 313, 342, 382, 1001
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 9 cut(s) 36, 111, 161, 466, 491, 781, 845, 1003, 1060
HpyCH4III ACNGT 4 cut(s) 217, 830, 998, 1269
HpyCH4IV ACGT 4 cut(s) 161, 248, 351, 430
HpyCH4V TGCA 8 cut(s) 154, 739, 765, 877, 893, 918, 965, 1112
HpyF10VI GCNNNNNNNGC 9 cut(s) 392, 401, 632, 745, 902, 971, 1040, 1175, 1208
HpyF3I CTNAG 4 cut(s) 119, 303, 797, 927
HpySE526I ACGT 4 cut(s) 161, 248, 351, 430
Hsp92II CATG 6 cut(s) 179, 425, 682, 890, 904, 1166
HspAI GCGC 1 cut(s) 300
Kpn2I TCCGGA 1 cut(s) 31
Kzo9I GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
LmnI GCTCC 2 cut(s) 91, 392
Lsp1109I GCAGC 5 cut(s) 566, 725, 732, 905, 949
LweI GCATC 5 cut(s) 37, 613, 763, 932, 949
MaeI CTAG 4 cut(s) 389, 699, 861, 1248
MaeII ACGT 4 cut(s) 161, 248, 351, 430
MaeIII GTNAC 5 cut(s) 96, 130, 275, 352, 431
MalI GATC 7 cut(s) 78, 144, 210, 308, 522, 945, 1227
MbiI CCGCTC 3 cut(s) 108, 125, 676
MboI GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
MboII GAAGA 5 cut(s) 221, 515, 1003, 1018, 1103
MflI RGATCY 1 cut(s) 208
MlsI TGGCCA 2 cut(s) 404, 905
MluCI AATT 4 cut(s) 406, 732, 950, 1243
MluNI TGGCCA 2 cut(s) 404, 905
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 3 cut(s) 113, 186, 236
Mox20I TGGCCA 2 cut(s) 404, 905
MroI TCCGGA 1 cut(s) 31
MscI TGGCCA 2 cut(s) 404, 905
MslI CAYNNNNRTG 2 cut(s) 45, 1161
Msp20I TGGCCA 2 cut(s) 404, 905
MspA1I CMGCKG 2 cut(s) 83, 896
MspI CCGG 8 cut(s) 32, 273, 280, 322, 491, 1134, 1203, 1256
MspR9I CCNGG 4 cut(s) 323, 346, 939, 1257
Mva1269I GAATGC 1 cut(s) 1045
MvaI CCWGG 2 cut(s) 346, 939
MvnI CGCG 1 cut(s) 957
MwoI GCNNNNNNNGC 9 cut(s) 392, 401, 632, 745, 902, 971, 1040, 1175, 1208
NciI CCSGG 2 cut(s) 323, 1257
NdeII GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
NlaIII CATG 6 cut(s) 179, 425, 682, 890, 904, 1166
NlaIV GGNNCC 5 cut(s) 87, 210, 419, 821, 1168
NmuCI GTSAC 3 cut(s) 96, 130, 352
NruI TCGCGA 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PctI GAATGC 1 cut(s) 1045
PfeI GAWTC 2 cut(s) 179, 445
Pfl23II CGTACG 2 cut(s) 261, 516
PflFI GACNNNGTC 1 cut(s) 94
PkrI GCNGC 8 cut(s) 85, 127, 271, 556, 715, 747, 895, 964
PleI GAGTC 1 cut(s) 127
PmaCI CACGTG 1 cut(s) 352
PmlI CACGTG 1 cut(s) 352
PpsI GAGTC 1 cut(s) 127
Ppu21I YACGTR 3 cut(s) 162, 249, 352
Psp6I CCWGG 2 cut(s) 344, 937
PspCI CACGTG 1 cut(s) 352
PspEI GGTNACC 1 cut(s) 275
PspFI CCCAGC 2 cut(s) 539, 1034
PspGI CCWGG 2 cut(s) 344, 937
PspLI CGTACG 2 cut(s) 261, 516
PspN4I GGNNCC 5 cut(s) 87, 210, 419, 821, 1168
PspPI GGNCC 4 cut(s) 418, 475, 488, 1167
PsuI RGATCY 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 896
RruI TCGCGA 1 cut(s) 957
RsaI GTAC 5 cut(s) 187, 238, 263, 383, 518
RsaNI GTAC 5 cut(s) 186, 237, 262, 382, 517
RseI CAYNNNNRTG 2 cut(s) 45, 1161
SalI GTCGAC 1 cut(s) 311
SatI GCNGC 8 cut(s) 84, 126, 270, 555, 714, 746, 894, 963
Sau3AI GATC 7 cut(s) 76, 142, 208, 306, 520, 943, 1225
Sau96I GGNCC 4 cut(s) 418, 475, 488, 1167
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 4 cut(s) 323, 346, 939, 1257
SfaNI GCATC 5 cut(s) 37, 613, 763, 932, 949
SfcI CTRYAG 1 cut(s) 1268
SinI GGWCC 1 cut(s) 418
SmiMI CAYNNNNRTG 2 cut(s) 45, 1161
SmlI CTYRAG 3 cut(s) 137, 253, 455
SmoI CTYRAG 3 cut(s) 137, 253, 455
Sse9I AATT 4 cut(s) 406, 732, 950, 1243
SsiI CCGC 7 cut(s) 83, 108, 125, 128, 270, 674, 1151
SspI AATATT 1 cut(s) 604
SspMI CTAG 4 cut(s) 389, 699, 861, 1248
StyD4I CCNGG 4 cut(s) 321, 344, 937, 1255
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 4 cut(s) 217, 830, 998, 1269
TaiI ACGT 4 cut(s) 164, 251, 354, 433
TaqI TCGA 3 cut(s) 75, 104, 312
TasI AATT 4 cut(s) 406, 732, 950, 1243
TauI GCSGC 3 cut(s) 86, 128, 272
TfiI GAWTC 2 cut(s) 179, 445
TscAI CASTG 2 cut(s) 835, 884
TseFI GTSAC 3 cut(s) 96, 130, 352
TseI GCWGC 5 cut(s) 554, 713, 745, 893, 962
Tsp45I GTSAC 3 cut(s) 96, 130, 352
TspDTI ATGAA 2 cut(s) 438, 553
TspRI CASTG 2 cut(s) 835, 884
Tth111I GACNNNGTC 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 418
XapI RAATTY 3 cut(s) 732, 950, 1243
XbaI TCTAGA 1 cut(s) 1247
XmaJI CCTAGG 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 4 cut(s) 389, 699, 861, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.