MD01G1035600.v1.1

LMBR1 domain-containing protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008090
Physical Location & Seq
Forward (+)
12010667 .. 12020048
9382 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1035600.v1.1.491

Sequence Viewer

Length: 2226 bp
ATGTGGGTCTTCTACCTGATCTCTCTGCCCCTGACCTTGGGCATGGTTCTTTTCACGCTCAAGTACTTCGCCGGCCCGGAGGTCCCTCGCTATGTTATGATCACCGTCGGGTACACCTGGTTCTGTTCTCTCTCCATCATCGTCATTGTGCCCGCCGACATCTGGACGACGATAAACCATATTGAGAGTGGAGGAATATCTTTCTTTTGGAGCTGGTCGTATTGGAGTACATTTTTATTAACTTGGACTGTGGTGCCCCTTATTCAGGGTTTTGAAGACGCTGGTGACTTCACCGTGACAGAGAGATTGAAGACTAGTGTGCATGTTAACTTGGTTTTCTATCTAATTCTGGGGTTTATTGGTCTTATTGGATTAATTCTTCTCATCAGCATGCACAAAAACTGGGGTGGAGGTGTTCTTGGTTTTGCCATGGGCTGCTCGAATACTTTTGGACTTGTTACAGGTGCATTTCTTCTTGGATTTGGTTTGAGTGAAATCCCAAAGGGCCTTTGGAAAAATTCCGATTGGACTATCCGCCAAAAAGTTCTATCTCATAAAATTGCCAAAATGGCTGTGAAACTTGACGACGCTCATCAAGATCTTTCAAATGCTATTGTGGTTGCCCAAGCAACATCCACTCAGATGTCCAAGCGCGATCCTTTGAGACCCTACATGGATATTATAGACAACATGTTAGCTCAAATGTTCAGGGAGGACCCATCCTTCAAACCACAAGGTGGCCGATTAGGTGAAAACGACATGGACTATGATACTGACGAAAAATCAATGGCAACACTTAGGCGTCATCTTCGGGGAGCCCGGGAAGAGTATTATCGGTACAAAAGTGAGTATATGACTTATGTTTTGGAGGCCCTTGAACTTGAAGATACTATAAAAAATTATGAACGCCGGAGTTCTACTGGATGGAAATATGTCTCAAGCTTCAGACCTACTCGAACTGGCACATTAGGATCCCTTGTTGATACAATAGAATTCTTATGGCGCTGCATACTAAGCAAAGAAGCTGAGAAACTTTTGGCTATCATACTTGGTATCATTTCAGTTGCTATTCTTTTAGCAGAGGCAACTCTTCTTCCTCGAGTTGATCTATCTCTCTTTTCAATTCTCATCAATTCTGTTGGAAAGCAAGAGGTTCTTGTACAGGTATTTGCCTTTGTTCCTTTGCTGTATATGTGCATCTGCACATACTACTCCTTGTTCAAAATTGGTATGTTGATGTTTTACTCATTAACACCCAGGCAAACAAGCTCAGTCAATTTGCTTATGATATGCTCGATGGTTGCTCGTTATGCTCCTCCAGTTTCATACAATTTCCTTAATCTCATTCGTCTTGGTGAACATAAGACTATATTTGAGAAGCGAATGGGAAACATTGATCAAGCAGTCCCTTTCTTTGGGAGTGAGTTTAACAGAATATATCCACTTATCATGGTTGTGTACACCCTGTTGGTTGCATGCAATTTTTTTGACCGCATGATTGATTTTTTTGGGAGCTGGAAGCGATTTAGGTTTCAAACCGAGGTTGATGATATGGATGGTTTTGATCCATCTGGATTAATTATCTTACAAAAGGAACGATCTTGGATTGAACAAGGTCTGAAAGTTGGTGAACATGTTATACCCCTAGCTAGGAATTTCAACAGCACGGATGTTGAGACGGGCAGCAACAACATGGATAGGACTCATGTTGAACTGAAAGCAACAACCAGTCTAGGCACTGAGGGTGCAAATGGAACTTCCTCAAAATCCTTGAGAGACGAATCTCGTATTAGATATGGCTCAAGCAAAGAAGCCATACTTAGATATGGCTCAAGCAAAGAAGCCATCAGCAACAAGTATGCTGCCATTAGAGATCAGAGCAGGCAATCATCTTCGAATACAAATCAGAATATTTCTGCTGTCAAGGTCTCCTTGCTTGATGAAGGGAACTCTAACCCTGATAGCACGGCAGGAGGGTTACATACTGGTTTGTCCTCAAAGTGGGAATCAATGAAAAACGGTTTCCAAAATTTCAAGGCAAACATAGCGGCCAAAAAATTTATTCCCTTGCACCAAGTTCAAGATACTGTAGAGCTATCTCGTGCTTCATCTAATGAGTCCCTCGATGAGATATTCCAGAGATTGAAACGGCGGTCGGTAGACCATGTGAGTTATGTTGACGAGGGTGAAGATGGCATGGAGGGGAAGTCTGGTCCCAGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LMBR1

Protein Analysis

742

Amino Acids

83.72

Weight (kDa)

7.18

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LMBR1 PF04791 3 - 495 9.2e-93 LMBR1-like membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 253
AccB7I CCANNNNNTGG 1 cut(s) 737
AccI GTMKAC 1 cut(s) 2160
AccII CGCG 1 cut(s) 654
AciI CCGC 5 cut(s) 153, 535, 1492, 2048, 2152
AclWI GGATC 4 cut(s) 650, 966, 979, 1559
AcoI YGGCCR 2 cut(s) 739, 2049
AcsI RAATTY 5 cut(s) 517, 992, 1654, 2029, 2057
AcuI CTGAAG 1 cut(s) 928
AcyI GRCGYC 1 cut(s) 802
AdeI CACNNNGTG 1 cut(s) 737
AfaI GTAC 6 cut(s) 65, 113, 229, 839, 1161, 1460
AfiI CCNNNNNNNGG 7 cut(s) 37, 162, 265, 737, 1415, 1650, 2001
AflIII ACRYGT 2 cut(s) 690, 1633
AhlI ACTAGT 1 cut(s) 314
AjnI CCWGG 2 cut(s) 116, 1256
AluBI AGCT 8 cut(s) 213, 698, 942, 1025, 1269, 1515, 1649, 2095
AluI AGCT 8 cut(s) 213, 698, 942, 1025, 1269, 1515, 1649, 2095
Alw26I GTCTC 5 cut(s) 658, 940, 1670, 1770, 1933
AlwI GGATC 4 cut(s) 650, 966, 979, 1559
Ama87I CYCGRG 2 cut(s) 819, 1098
AoxI GGCC 5 cut(s) 73, 505, 739, 870, 2049
ApeKI GCWGC 4 cut(s) 435, 1005, 1683, 1862
ApoI RAATTY 5 cut(s) 517, 992, 1654, 2029, 2057
ArsI GACNNNNNNTTYG 2 cut(s) 847, 879
AseI ATTAAT 2 cut(s) 374, 1577
AspLEI GCGC 2 cut(s) 654, 1005
AspS9I GGNCC 6 cut(s) 74, 82, 505, 715, 871, 2213
AsuC2I CCSGG 3 cut(s) 77, 820, 821
AsuHPI GGTGA 7 cut(s) 94, 283, 296, 761, 1367, 1640, 2198
AsuII TTCGAA 1 cut(s) 1895
AvaI CYCGRG 2 cut(s) 819, 1098
AvaII GGWCC 3 cut(s) 82, 715, 2213
BaeGI GKGCMC 2 cut(s) 153, 258
BaeI ACNNNNGTAYC 2 cut(s) 829, 862
BamHI GGATCC 1 cut(s) 971
BanI GGYRCC 1 cut(s) 253
BanII GRGCYC 1 cut(s) 820
BauI CACGAG 1 cut(s) 2100
BbsI GAAGAC 2 cut(s) 282, 317
BbvI GCAGC 4 cut(s) 422, 992, 1695, 1849
BccI CCATC 8 cut(s) 143, 727, 918, 1291, 1550, 1576, 1853, 2186
BceAI ACGGC 2 cut(s) 1983, 2165
BciT130I CCWGG 2 cut(s) 118, 1258
BclI TGATCA 2 cut(s) 99, 1396
BcnI CCSGG 3 cut(s) 77, 820, 821
BcoDI GTCTC 5 cut(s) 658, 940, 1670, 1770, 1933
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 4 cut(s) 315, 1646, 1650, 1733
BfmI CTRYAG 1 cut(s) 2088
BfoI RGCGCY 1 cut(s) 1006
BglI GCCNNNNNGGC 1 cut(s) 569
BglII AGATCT 1 cut(s) 598
BisI GCNGC 5 cut(s) 436, 1006, 1684, 1863, 2049
BlsI GCNGC 5 cut(s) 437, 1007, 1685, 1864, 2050
BmcAI AGTACT 1 cut(s) 65
Bme1390I CCNGG 5 cut(s) 77, 118, 820, 821, 1258
Bme18I GGWCC 3 cut(s) 82, 715, 2213
BmeT110I CYCGRG 2 cut(s) 819, 1098
BmgT120I GGNCC 6 cut(s) 74, 82, 505, 715, 871, 2213
BmiI GGNNCC 6 cut(s) 84, 255, 717, 817, 973, 2215
BmrFI CCNGG 5 cut(s) 77, 118, 820, 821, 1258
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 1 cut(s) 1206
BmuI ACTGGG 1 cut(s) 412
BpiI GAAGAC 2 cut(s) 282, 317
BpmI CTGGAG 1 cut(s) 1302
Bpu14I TTCGAA 1 cut(s) 1895
BpuEI CTTGAG 5 cut(s) 44, 922, 1786, 1792, 1816
BpuMI CCSGG 3 cut(s) 77, 820, 821
BsaHI GRCGYC 1 cut(s) 802
BsaI GGTCTC 2 cut(s) 658, 1933
BsaJI CCNNGG 5 cut(s) 36, 429, 819, 1256, 1539
BsaXI ACNNNNNCTCC 2 cut(s) 2192, 2222
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 162, 265, 737, 1415, 1650, 2001
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 6 cut(s) 407, 925, 964, 1319, 1728, 1990
BseBI CCWGG 2 cut(s) 118, 1258
BseDI CCNNGG 5 cut(s) 36, 429, 819, 1256, 1539
BseGI GGATG 5 cut(s) 632, 719, 929, 1561, 1675
BseLI CCNNNNNNNGG 7 cut(s) 37, 162, 265, 737, 1415, 1650, 2001
BseMII CTCAG 4 cut(s) 653, 1017, 1284, 1731
BseNI ACTGG 6 cut(s) 407, 925, 964, 1319, 1728, 1990
BseRI GAGGAG 1 cut(s) 1305
BseSI GKGCMC 2 cut(s) 153, 258
BseXI GCAGC 4 cut(s) 422, 992, 1695, 1849
BseYI CCCAGC 1 cut(s) 2216
BsgI GTGCAG 1 cut(s) 1186
Bsh1236I CGCG 1 cut(s) 654
Bsh1285I CGRYCG 1 cut(s) 2156
BshFI GGCC 5 cut(s) 75, 507, 741, 872, 2051
BshNI GGYRCC 1 cut(s) 253
BsiEI CGRYCG 1 cut(s) 2156
BsiHKCI CYCGRG 2 cut(s) 819, 1098
BsiSI CCGG 4 cut(s) 72, 77, 820, 910
BslFI GGGAC 4 cut(s) 68, 1391, 2104, 2199
BslI CCNNNNNNNGG 7 cut(s) 37, 162, 265, 737, 1415, 1650, 2001
BsmAI GTCTC 5 cut(s) 658, 940, 1670, 1770, 1933
BsmBI CGTCTC 2 cut(s) 1670, 1770
BsmFI GGGAC 4 cut(s) 68, 1391, 2104, 2199
BsnI GGCC 5 cut(s) 75, 507, 741, 872, 2051
Bso31I GGTCTC 2 cut(s) 658, 1933
BsoBI CYCGRG 2 cut(s) 819, 1098
Bsp119I TTCGAA 1 cut(s) 1895
Bsp1286I GDGCHC 3 cut(s) 153, 258, 820
Bsp1407I TGTACA 2 cut(s) 1159, 1458
Bsp19I CCATGG 1 cut(s) 429
BspACI CCGC 5 cut(s) 153, 535, 1492, 2048, 2152
BspANI GGCC 5 cut(s) 75, 507, 741, 872, 2051
BspCNI CTCAG 4 cut(s) 652, 1018, 1283, 1732
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 6 cut(s) 84, 255, 717, 817, 973, 2215
BspPI GGATC 4 cut(s) 650, 966, 979, 1559
BspT104I TTCGAA 1 cut(s) 1895
BspT107I GGYRCC 1 cut(s) 253
BspTNI GGTCTC 2 cut(s) 658, 1933
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 2 cut(s) 1159, 1458
BsrI ACTGG 6 cut(s) 407, 925, 964, 1319, 1728, 1990
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 5 cut(s) 36, 429, 819, 1256, 1539
BssNI GRCGYC 1 cut(s) 802
BssSI CACGAG 1 cut(s) 2100
BssT1I CCWWGG 2 cut(s) 36, 429
Bst2BI CACGAG 1 cut(s) 2100
Bst2UI CCWGG 2 cut(s) 118, 1258
Bst4CI ACNGT 5 cut(s) 106, 250, 295, 2021, 2089
Bst6I CTCTTC 2 cut(s) 819, 1095
BstACI GRCGYC 1 cut(s) 802
BstAUI TGTACA 2 cut(s) 1159, 1458
BstBI TTCGAA 1 cut(s) 1895
BstC8I GCNNGC 5 cut(s) 73, 153, 392, 1477, 1883
BstDEI CTNAG 7 cut(s) 639, 797, 1013, 1026, 1270, 1740, 1820
BstDSI CCRYGG 1 cut(s) 429
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 5 cut(s) 632, 719, 929, 1561, 1675
BstFNI CGCG 1 cut(s) 654
BstH2I RGCGCY 1 cut(s) 1006
BstHHI GCGC 2 cut(s) 654, 1005
BstMAI GTCTC 5 cut(s) 658, 940, 1670, 1770, 1933
BstMCI CGRYCG 1 cut(s) 2156
BstMWI GCNNNNNNNGC 4 cut(s) 569, 1014, 1310, 2045
BstNI CCWGG 2 cut(s) 118, 1258
BstNSI RCATGY 5 cut(s) 326, 394, 694, 1479, 1637
BstSCI CCNGG 5 cut(s) 75, 116, 818, 819, 1256
BstSFI CTRYAG 1 cut(s) 2088
BstSLI GKGCMC 2 cut(s) 153, 258
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 4 cut(s) 422, 992, 1695, 1849
BstV2I GAAGAC 2 cut(s) 282, 317
BstX2I RGATCY 2 cut(s) 598, 971
BstYI RGATCY 2 cut(s) 598, 971
BsuRI GGCC 5 cut(s) 75, 507, 741, 872, 2051
BtgI CCRYGG 1 cut(s) 429
BtsCI GGATG 5 cut(s) 632, 719, 929, 1561, 1675
BtsIMutI CAGTG 1 cut(s) 1737
Cac8I GCNNGC 5 cut(s) 73, 153, 392, 1477, 1883
CfoI GCGC 2 cut(s) 654, 1005
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 6 cut(s) 74, 82, 505, 715, 871, 2213
Cfr9I CCCGGG 1 cut(s) 819
CseI GACGC 3 cut(s) 287, 596, 791
CsiI ACCWGGT 1 cut(s) 116
Csp6I GTAC 6 cut(s) 64, 112, 228, 838, 1160, 1459
CviQI GTAC 6 cut(s) 64, 112, 228, 838, 1160, 1459
DdeI CTNAG 7 cut(s) 639, 797, 1013, 1026, 1270, 1740, 1820
DraIII CACNNNGTG 1 cut(s) 737
EaeI YGGCCR 2 cut(s) 739, 2049
Eam1104I CTCTTC 2 cut(s) 819, 1095
EarI CTCTTC 2 cut(s) 819, 1095
EciI GGCGGA 1 cut(s) 524
Eco130I CCWWGG 2 cut(s) 36, 429
Eco24I GRGCYC 1 cut(s) 820
Eco31I GGTCTC 2 cut(s) 658, 1933
Eco47I GGWCC 3 cut(s) 82, 715, 2213
Eco57I CTGAAG 1 cut(s) 928
Eco88I CYCGRG 2 cut(s) 819, 1098
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoO109I RGGNCCY 4 cut(s) 82, 505, 715, 871
EcoRI GAATTC 1 cut(s) 992
EcoRII CCWGG 2 cut(s) 116, 1256
EcoT14I CCWWGG 2 cut(s) 36, 429
EcoT38I GRGCYC 1 cut(s) 820
ErhI CCWWGG 2 cut(s) 36, 429
Esp3I CGTCTC 2 cut(s) 1670, 1770
FalI AAGNNNNNCTT 6 cut(s) 1140, 1172, 1803, 1835, 1916, 1948
FaqI GGGAC 4 cut(s) 68, 1391, 2104, 2199
FauI CCCGC 1 cut(s) 160
FbaI TGATCA 2 cut(s) 99, 1396
FblI GTMKAC 1 cut(s) 2160
Fnu4HI GCNGC 5 cut(s) 436, 1006, 1684, 1863, 2049
FokI GGATG 5 cut(s) 619, 706, 936, 1568, 1682
FriOI GRGCYC 1 cut(s) 820
Fsp4HI GCNGC 5 cut(s) 436, 1006, 1684, 1863, 2049
FspBI CTAG 4 cut(s) 315, 1646, 1650, 1733
GlaI GCGC 2 cut(s) 653, 1004
GluI GCNGC 5 cut(s) 436, 1006, 1684, 1863, 2049
GsaI CCCAGC 1 cut(s) 2220
GsuI CTGGAG 1 cut(s) 1302
HaeII RGCGCY 1 cut(s) 1006
HaeIII GGCC 5 cut(s) 75, 507, 741, 872, 2051
HapII CCGG 4 cut(s) 72, 77, 820, 910
HgaI GACGC 3 cut(s) 287, 596, 791
HhaI GCGC 2 cut(s) 654, 1005
Hin1I GRCGYC 1 cut(s) 802
Hin6I GCGC 2 cut(s) 652, 1003
HinP1I GCGC 2 cut(s) 652, 1003
HincII GTYRAC 2 cut(s) 328, 2179
HindII GTYRAC 2 cut(s) 328, 2179
HindIII AAGCTT 1 cut(s) 940
HinfI GANTC 4 cut(s) 1702, 1781, 2006, 2117
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 4 cut(s) 72, 77, 820, 910
HphI GGTGA 7 cut(s) 94, 283, 296, 761, 1367, 1640, 2198
Hpy166II GTNNAC 8 cut(s) 114, 328, 1358, 1459, 1461, 1631, 2161, 2179
Hpy188I TCNGA 6 cut(s) 523, 642, 947, 1620, 1878, 1908
Hpy188III TCNNGA 5 cut(s) 163, 596, 1572, 2081, 2137
Hpy8I GTNNAC 8 cut(s) 114, 328, 1358, 1459, 1461, 1631, 2161, 2179
Hpy99I CGWCG 3 cut(s) 110, 172, 590
HpyAV CCTTC 2 cut(s) 733, 1937
HpyCH4III ACNGT 5 cut(s) 106, 250, 295, 2021, 2089
HpyF10VI GCNNNNNNNGC 4 cut(s) 569, 1014, 1310, 2045
HpyF3I CTNAG 7 cut(s) 639, 797, 1013, 1026, 1270, 1740, 1820
Hsp92I GRCGYC 1 cut(s) 802
HspAI GCGC 2 cut(s) 652, 1003
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Ksp22I TGATCA 2 cut(s) 99, 1396
KspAI GTTAAC 1 cut(s) 328
LmnI GCTCC 4 cut(s) 210, 815, 1318, 1512
Lsp1109I GCAGC 4 cut(s) 422, 992, 1695, 1849
LweI GCATC 1 cut(s) 1206
MabI ACCWGGT 1 cut(s) 116
MaeI CTAG 4 cut(s) 315, 1646, 1650, 1733
MaeIII GTNAC 4 cut(s) 284, 295, 457, 1977
MflI RGATCY 2 cut(s) 598, 971
MhlI GDGCHC 3 cut(s) 153, 258, 820
MlyI GAGTC 2 cut(s) 1696, 2126
MmeI TCCRAC 1 cut(s) 1120
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 7 cut(s) 239, 327, 374, 1250, 1338, 1428, 1577
MslI CAYNNNNRTG 3 cut(s) 389, 641, 1454
MspI CCGG 4 cut(s) 72, 77, 820, 910
MspR9I CCNGG 5 cut(s) 77, 118, 820, 821, 1258
MvaI CCWGG 2 cut(s) 118, 1258
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 4 cut(s) 569, 1014, 1310, 2045
NaeI GCCGGC 1 cut(s) 73
NciI CCSGG 3 cut(s) 77, 820, 821
NcoI CCATGG 1 cut(s) 429
NgoMIV GCCGGC 1 cut(s) 71
NlaIV GGNNCC 6 cut(s) 84, 255, 717, 817, 973, 2215
NmuCI GTSAC 2 cut(s) 284, 295
NspI RCATGY 5 cut(s) 326, 394, 694, 1479, 1637
NspV TTCGAA 1 cut(s) 1895
PaeI GCATGC 2 cut(s) 394, 1479
PaeR7I CTCGAG 1 cut(s) 1098
PciI ACATGT 2 cut(s) 690, 1633
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 2 cut(s) 1781, 2006
PflMI CCANNNNNTGG 1 cut(s) 737
PkrI GCNGC 5 cut(s) 437, 1007, 1685, 1864, 2050
PleI GAGTC 2 cut(s) 1696, 2125
PpsI GAGTC 2 cut(s) 1696, 2125
PpuMI RGGWCCY 2 cut(s) 82, 715
PscI ACATGT 2 cut(s) 690, 1633
PshBI ATTAAT 2 cut(s) 374, 1577
Psp5II RGGWCCY 2 cut(s) 82, 715
Psp6I CCWGG 2 cut(s) 116, 1256
PspFI CCCAGC 1 cut(s) 2216
PspGI CCWGG 2 cut(s) 116, 1256
PspN4I GGNNCC 6 cut(s) 84, 255, 717, 817, 973, 2215
PspPI GGNCC 6 cut(s) 74, 82, 505, 715, 871, 2213
PspPPI RGGWCCY 2 cut(s) 82, 715
PspXI VCTCGAGB 1 cut(s) 1098
PsrI GAACNNNNNNTAC 4 cut(s) 104, 136, 1623, 1655
PsuI RGATCY 2 cut(s) 598, 971
RsaI GTAC 6 cut(s) 65, 113, 229, 839, 1161, 1460
RsaNI GTAC 6 cut(s) 64, 112, 228, 838, 1160, 1459
RseI CAYNNNNRTG 3 cut(s) 389, 641, 1454
SaqAI TTAA 7 cut(s) 239, 327, 374, 1250, 1338, 1428, 1577
SatI GCNGC 5 cut(s) 436, 1006, 1684, 1863, 2049
Sau96I GGNCC 6 cut(s) 74, 82, 505, 715, 871, 2213
ScaI AGTACT 1 cut(s) 65
SchI GAGTC 2 cut(s) 1696, 2126
ScrFI CCNGG 5 cut(s) 77, 118, 820, 821, 1258
SduI GDGCHC 3 cut(s) 153, 258, 820
SexAI ACCWGGT 1 cut(s) 116
SfaNI GCATC 1 cut(s) 1206
SfcI CTRYAG 1 cut(s) 2088
Sfr274I CTCGAG 1 cut(s) 1098
SfuI TTCGAA 1 cut(s) 1895
SinI GGWCC 3 cut(s) 82, 715, 2213
SlaI CTCGAG 1 cut(s) 1098
SmaI CCCGGG 1 cut(s) 821
SmiMI CAYNNNNRTG 3 cut(s) 389, 641, 1454
SmlI CTYRAG 6 cut(s) 59, 937, 1098, 1771, 1801, 1831
SmoI CTYRAG 6 cut(s) 59, 937, 1098, 1771, 1801, 1831
SpeI ACTAGT 1 cut(s) 314
SphI GCATGC 2 cut(s) 394, 1479
SsiI CCGC 5 cut(s) 153, 535, 1492, 2048, 2152
SspI AATATT 1 cut(s) 1912
SspMI CTAG 4 cut(s) 315, 1646, 1650, 1733
StyD4I CCNGG 5 cut(s) 75, 116, 818, 819, 1256
StyI CCWWGG 2 cut(s) 36, 429
TaaI ACNGT 5 cut(s) 106, 250, 295, 2021, 2089
TaqI TCGA 6 cut(s) 440, 955, 1099, 1295, 1895, 2124
TatI WGTACW 4 cut(s) 63, 227, 1159, 1458
TauI GCSGC 1 cut(s) 2051
TfiI GAWTC 2 cut(s) 1781, 2006
Tru1I TTAA 7 cut(s) 239, 327, 374, 1250, 1338, 1428, 1577
Tru9I TTAA 7 cut(s) 239, 327, 374, 1250, 1338, 1428, 1577
TscAI CASTG 1 cut(s) 1744
TseFI GTSAC 2 cut(s) 284, 295
TseI GCWGC 4 cut(s) 435, 1005, 1683, 1862
Tsp45I GTSAC 2 cut(s) 284, 295
TspDTI ATGAA 5 cut(s) 918, 1314, 1956, 2027, 2097
TspGWI ACGGA 1 cut(s) 1682
TspMI CCCGGG 1 cut(s) 819
TspRI CASTG 1 cut(s) 1744
Van91I CCANNNNNTGG 1 cut(s) 737
VpaK11BI GGWCC 3 cut(s) 82, 715, 2213
VspI ATTAAT 2 cut(s) 374, 1577
XagI CCTNNNNNAGG 1 cut(s) 263
XapI RAATTY 5 cut(s) 517, 992, 1654, 2029, 2057
XceI RCATGY 5 cut(s) 326, 394, 694, 1479, 1637
XcmI CCANNNNNNNNNTGG 2 cut(s) 185, 507
XhoI CTCGAG 1 cut(s) 1098
XmaI CCCGGG 1 cut(s) 819
XmiI GTMKAC 1 cut(s) 2160
XspI CTAG 4 cut(s) 315, 1646, 1650, 1733
ZrmI AGTACT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.