Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006310

Physical Location & Seq

Genomic Coordinates Reverse (-)

20598796 .. 20603769

Gene Structure 4974 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1091000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGGATCACGGCGACCCCAACCAGTCCCGCCTCATCGGAGACTACATACTGGGCCCCAAAGTCGGGTCGGGTTCCTTCGCGGTTGTTTGGCGCTCACGGCACCGACAACTCGGTACTGAAGTTGCCGTCAAAGAAATCGACAAGAAGCCGCTCAGCCCAAAAGTCAGCGACAACGTACTCAAAGAAATATCCATTCTCAGCACCATCAACCACCCCAACATCATTCGCCTTTTCGAGGCCATCCAGACCGAGGACAGAATCTTCCTTGTGCTGGAGTACTGTGACGGCGGCGACCTGGCGGATTATATTCGCCGACATGGGAAGGTTTCAGAAACTGTTGCCAGGCACTTTATGAGGCAATTGGCTGCAGGACTGCAAGTGCTTCAAGAAAAGCACCTTATTCATAGGGATTTAAAACCGCAGAACTTACTCTTGTCAACGACAGTTGAAGATACCCCACATTTGAAGATTGGGGATTTTGGCTTTGCAAGATCCCTCATTGGGGGCTTGGCTGACACACTATGTGGTTCACCACTGTACATGGCTCCAGAGATTATTCAGAACCAAAAGTATGATGCAAAGGCCGATTTGTGGAGTGTTGGAGCAATTTTGTTTCAGCTGGTGACTGGGAAGCCACCATTTGATGGGAATAATCAATTACAGCTTTTCCAAAATATTCTGACATCGACTGAGCTGAGGTTTCCAGAAGGTGCTTTGGAAGAATTGCATCCAGATTGTGTAGATCTCTGCAGAAGCCTGTTACGTCTAAATCCAGTTGAGCGACTATCATTTACGGAGTTCTTCAATCACAAATTCCTCAGAGATTCTAGGTATCAAGTGGATATTGGTCACCCTTCAGTGCTTCCACCACTTCAATCAACGGTCGAACAGCCTAGTTCTTCTGTCTCCGACAATATATTGCAAATGCATTTCTGGCAAACTACCAACTCATCTATTACCCGAGATCCAAAGTCGCAGGATCAGCTTCGAGTTTCTGATTCAATGGAGTCCATCGAGAAGGACTATGTTATGGTCAATTCTTTCTCTCATTACCTTGAATCTTCCCTGCCACTTCATTCCACAACTGGAGCTTCTCTCAGTGCTTCAAAGCAGAAAGATCGTGATAAAACAGTTATAGTGAAAACCGAGGAGCAGGTAGCTAGTTCTGTGGGTGCAGAAAGTTCACAAACTAGTGGATCAGCTAAATTACCAGCATCTTGTGAATCAACACTGTTACTAGAAGTGCAAGGACTATCTATACTGCATCCTTCTACTAGGCTCCATCTTTTACATCAGTATGCTCAGGTTCTTGCAGACCTGTCGCAGGAAAAGTACAATGCAGGACTATTTCTAGACTCGTTTTCGGTTCAACTTCTTGTTTTGGCCATATGGAAGAAATCTATTCAAATTTGCAGCACTTGGCTGGCATCATCTAAAGAGGATAAATCACCTAAAGGTAGCTCAGCTAATGAATCCTTGCAGGGAGGTGCAGCTTCATCAACAAACGCCTCAGAAAATGTAGATTTTAGCCAGCCTTCATCTGTTTTGATGTGGGCAGAGCAAGGTTTCATTGTTGCTGTTGATCGAGCAGAGAAGCTATCCTATCATATCCAAGAAATGGATGGTGCTGCTGAATTGCCGGATGCCATGGAAATTATTTACCAAAAAGCGCTCAAAGTTGGCACAAGTGGTGCTGTAGACGAGTACATGGAGAACAAAGGCAGTGCTGAAGCATTGTACTCCAAGGCTATGCTTCTCCTTTCTTTTATTGCAGGAGAAGCAACGTCTCTGCCACTAACACCTCCGTTTTCCTTAACTCCTGCCAACAAGAAGCGAATCCAACAATATATTCTGAACTTGGAGTCCCGTCGGGTCAACTTTCTGAAATCACAGCCTGCTCCTGTGCAAACCCGCGGCTCCTCGGCAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006914 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0061919

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04139 ko04140 ko04150 ko04212 map04136 map04138 map04139 map04140 map04150 map04212

Pfam Domains

Protein Families

Pkinase

Protein Analysis

644

Amino Acids

71.08

Weight (kDa)

5.94

Isoelectric Point (pI)

49.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	17 - 273	2.3e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	19 - 267	2.8e-43	Protein tyrosine and serine/threonine kinase
MIT_ATG1	PF24497	410 - 538	3.9e-46	ATG1, MIT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61960 AT3G61960
fragaria_vesca	FvH4_7g17860 FvH4_7g17860 FvH4_7g17860 FvH4_7g17860 FvH4_7g17860
malus_domestica	MD01G1091000.v1.1 MD07G1161200.v1.1
prunus_persica	Prupe.2G200500_v2.0.a1 Prupe.2G200500_v2.0.a1 Prupe.2G200500_v2.0.a1 Prupe.2G200500_v2.0.a1 Prupe.2G200500_v2.0.a1 Prupe.2G200500_v2.0.a1
pyrus_communis	pycom01g11510
rosa_chinensis	RchiOBHm_Chr1g0361311
rosa_laevigata	RLG00000027726
rosa_multiflora	Rmu_sc0002119.1_g000030
rosa_roxburghii	Rroxscaffold_4G00295060
rosa_rugosa	Rorug01G0288200
rosa_samantha	Rh1AG299400 Rh1BG263200 Rh1CG280700 Rh1DG293300 Rh4BG414700
rosa_wichuraiana	Rw1G026490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1144
AccB1I	GGYRCC	1 cut(s)	99
AccB7I	CCANNNNNTGG	2 cut(s)	644, 1618
AccBSI	CCGCTC	1 cut(s)	151
AccI	GTMKAC	1 cut(s)	1698
AccII	CGCG	2 cut(s)	80, 1914
AciI	CCGC	8 cut(s)	28, 80, 149, 288, 299, 419, 1912, 1914
AclWI	GGATC	5 cut(s)	12, 486, 959, 987, 1204
AcoI	YGGCCR	1 cut(s)	1383
AcsI	RAATTY	2 cut(s)	812, 1407
AcuI	CTGAAG	3 cut(s)	138, 840, 1749
AdeI	CACNNNGTG	1 cut(s)	524
AfaI	GTAC	7 cut(s)	115, 177, 278, 539, 1334, 1706, 1739
AfeI	AGCGCT	1 cut(s)	1671
AhlI	ACTAGT	1 cut(s)	1190
AjnI	CCWGG	2 cut(s)	294, 341
AjuI	GAANNNNNNNTTGG	2 cut(s)	1833, 1865
AloI	GAACNNNNNNTCC	2 cut(s)	1847, 1879
Alw26I	GTCTC	3 cut(s)	33, 910, 1791
AlwI	GGATC	5 cut(s)	12, 486, 959, 987, 1204
AlwNI	CAGNNNCTG	1 cut(s)	335
Ama87I	CYCGRG	1 cut(s)	960
Aor51HI	AGCGCT	1 cut(s)	1671
AoxI	GGCC	4 cut(s)	52, 237, 582, 1383
ApaI	GGGCCC	1 cut(s)	56
ApeKI	GCWGC	4 cut(s)	365, 1413, 1490, 1628
ApoI	RAATTY	2 cut(s)	812, 1407
AspLEI	GCGC	2 cut(s)	93, 1672
AspS9I	GGNCC	2 cut(s)	52, 53
AsuHPI	GGTGA	4 cut(s)	522, 634, 842, 1440
AvaI	CYCGRG	1 cut(s)	960
BaeGI	GKGCMC	1 cut(s)	56
BaeI	ACNNNNGTAYC	2 cut(s)	105, 138
BalI	TGGCCA	1 cut(s)	1385
BanI	GGYRCC	1 cut(s)	99
BanII	GRGCYC	1 cut(s)	56
BbvCI	CCTCAGC	1 cut(s)	695
BbvI	GCAGC	4 cut(s)	352, 1425, 1502, 1615
BccI	CCATC	6 cut(s)	212, 248, 638, 1019, 1290, 1616
BceAI	ACGGC	4 cut(s)	25, 110, 113, 301
BcgI	CGANNNNNNTGC	4 cut(s)	1100, 1134, 1302, 1336
BciT130I	CCWGG	2 cut(s)	296, 343
BcoDI	GTCTC	3 cut(s)	33, 910, 1791
BcuI	ACTAGT	1 cut(s)	1190
BfaI	CTAG	7 cut(s)	828, 894, 1161, 1191, 1238, 1275, 1352
BfmI	CTRYAG	3 cut(s)	366, 748, 1695
BfoI	RGCGCY	2 cut(s)	94, 1673
BfuAI	ACCTGC	1 cut(s)	1144
BglII	AGATCT	1 cut(s)	742
BisI	GCNGC	7 cut(s)	149, 289, 366, 1414, 1491, 1629, 1915
BlpI	GCTNAGC	2 cut(s)	152, 1462
BlsI	GCNGC	7 cut(s)	150, 290, 367, 1415, 1492, 1630, 1916
BmcAI	AGTACT	1 cut(s)	278
Bme1390I	CCNGG	2 cut(s)	296, 343
BmeT110I	CYCGRG	1 cut(s)	960
BmgT120I	GGNCC	2 cut(s)	52, 53
BmiI	GGNNCC	7 cut(s)	54, 55, 73, 101, 546, 1280, 1918
BmrFI	CCNGG	2 cut(s)	296, 343
BmrI	ACTGGG	2 cut(s)	59, 636
BmsI	GCATC	6 cut(s)	565, 736, 1223, 1273, 1436, 1633
BmuI	ACTGGG	2 cut(s)	59, 636
BplI	GAGNNNNNCTC	2 cut(s)	1080, 1112
BpmI	CTGGAG	3 cut(s)	293, 531, 1107
Bpu10I	CCTNAGC	2 cut(s)	695, 1302
Bpu1102I	GCTNAGC	2 cut(s)	152, 1462
BsaJI	CCNNGG	6 cut(s)	249, 1146, 1647, 1743, 1912, 1920
Bse1I	ACTGG	5 cut(s)	22, 54, 631, 773, 1090
BseBI	CCWGG	2 cut(s)	296, 343
BseDI	CCNNGG	6 cut(s)	249, 1146, 1647, 1743, 1912, 1920
BseGI	GGATG	5 cut(s)	240, 727, 1264, 1627, 1648
BseMII	CTCAG	9 cut(s)	166, 211, 681, 686, 832, 1111, 1316, 1476, 1524
BseNI	ACTGG	5 cut(s)	22, 54, 631, 773, 1090
BseRI	GAGGAG	2 cut(s)	1163, 1909
BseSI	GKGCMC	1 cut(s)	56
BseXI	GCAGC	4 cut(s)	352, 1425, 1502, 1615
BsgI	GTGCAG	2 cut(s)	1194, 1509
Bsh1236I	CGCG	2 cut(s)	80, 1914
Bsh1285I	CGRYCG	1 cut(s)	885
BshFI	GGCC	4 cut(s)	54, 239, 584, 1385
BshNI	GGYRCC	1 cut(s)	99
BsiEI	CGRYCG	1 cut(s)	885
BsiHKCI	CYCGRG	1 cut(s)	960
BsiSI	CCGG	1 cut(s)	1640
BslFI	GGGAC	2 cut(s)	10, 1849
BsmAI	GTCTC	3 cut(s)	33, 910, 1791
BsmBI	CGTCTC	1 cut(s)	1791
BsmFI	GGGAC	2 cut(s)	10, 1849
BsnI	GGCC	4 cut(s)	54, 239, 584, 1385
BsoBI	CYCGRG	1 cut(s)	960
Bsp120I	GGGCCC	1 cut(s)	52
Bsp1286I	GDGCHC	1 cut(s)	56
Bsp1407I	TGTACA	1 cut(s)	537
Bsp143I	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
Bsp1720I	GCTNAGC	2 cut(s)	152, 1462
Bsp19I	CCATGG	1 cut(s)	1647
BspACI	CCGC	8 cut(s)	28, 80, 149, 288, 299, 419, 1912, 1914
BspANI	GGCC	4 cut(s)	54, 239, 584, 1385
BspCNI	CTCAG	9 cut(s)	165, 210, 682, 687, 831, 1110, 1315, 1475, 1523
BspFNI	CGCG	2 cut(s)	80, 1914
BspLI	GGNNCC	7 cut(s)	54, 55, 73, 101, 546, 1280, 1918
BspMAI	CTGCAG	2 cut(s)	370, 752
BspMI	ACCTGC	1 cut(s)	1144
BspPI	GGATC	5 cut(s)	12, 486, 959, 987, 1204
BspT107I	GGYRCC	1 cut(s)	99
BsrBI	CCGCTC	1 cut(s)	151
BsrGI	TGTACA	1 cut(s)	537
BsrI	ACTGG	5 cut(s)	22, 54, 631, 773, 1090
BssECI	CCNNGG	6 cut(s)	249, 1146, 1647, 1743, 1912, 1920
BssMI	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
BssT1I	CCWWGG	2 cut(s)	1647, 1743
Bst2UI	CCWGG	2 cut(s)	296, 343
Bst4CI	ACNGT	7 cut(s)	281, 337, 445, 537, 883, 1132, 1233
BstAUI	TGTACA	1 cut(s)	537
BstC8I	GCNNGC	3 cut(s)	1425, 1532, 1896
BstDEI	CTNAG	9 cut(s)	152, 197, 690, 695, 818, 1097, 1302, 1462, 1510
BstDSI	CCRYGG	2 cut(s)	1647, 1912
BstEII	GGTNACC	1 cut(s)	848
BstENI	CCTNNNNNAGG	2 cut(s)	233, 1322
BstF5I	GGATG	5 cut(s)	240, 727, 1264, 1627, 1648
BstFNI	CGCG	2 cut(s)	80, 1914
BstH2I	RGCGCY	2 cut(s)	94, 1673
BstHHI	GCGC	2 cut(s)	93, 1672
BstKTI	GATC	8 cut(s)	7, 494, 745, 967, 982, 1120, 1199, 1585
BstMAI	GTCTC	3 cut(s)	33, 910, 1791
BstMBI	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
BstMCI	CGRYCG	1 cut(s)	885
BstMWI	GCNNNNNNNGC	4 cut(s)	97, 934, 982, 1778
BstNI	CCWGG	2 cut(s)	296, 343
BstPI	GGTNACC	1 cut(s)	848
BstSCI	CCNGG	2 cut(s)	294, 341
BstSFI	CTRYAG	3 cut(s)	366, 748, 1695
BstSLI	GKGCMC	1 cut(s)	56
BstUI	CGCG	2 cut(s)	80, 1914
BstV1I	GCAGC	4 cut(s)	352, 1425, 1502, 1615
BstX2I	RGATCY	3 cut(s)	491, 742, 964
BstYI	RGATCY	3 cut(s)	491, 742, 964
BsuRI	GGCC	4 cut(s)	54, 239, 584, 1385
BtgI	CCRYGG	2 cut(s)	1647, 1912
BtsCI	GGATG	5 cut(s)	240, 727, 1264, 1627, 1648
BtsI	GCAGTG	1 cut(s)	1729
BtsIMutI	CAGTG	5 cut(s)	533, 864, 1105, 1229, 1729
BveI	ACCTGC	1 cut(s)	1144
Cac8I	GCNNGC	3 cut(s)	1425, 1532, 1896
CaiI	CAGNNNCTG	1 cut(s)	335
CfoI	GCGC	2 cut(s)	93, 1672
Cfr13I	GGNCC	2 cut(s)	52, 53
Cfr42I	CCGCGG	1 cut(s)	1915
Csp6I	GTAC	7 cut(s)	114, 176, 277, 538, 1333, 1705, 1738
CviAII	CATG	4 cut(s)	317, 541, 1648, 1708
CviQI	GTAC	7 cut(s)	114, 176, 277, 538, 1333, 1705, 1738
DdeI	CTNAG	9 cut(s)	152, 197, 690, 695, 818, 1097, 1302, 1462, 1510
DpnI	GATC	8 cut(s)	6, 493, 744, 966, 981, 1119, 1198, 1584
DpnII	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
DraI	TTTAAA	1 cut(s)	414
DraIII	CACNNNGTG	1 cut(s)	524
EaeI	YGGCCR	1 cut(s)	1383
EciI	GGCGGA	1 cut(s)	314
Eco130I	CCWWGG	2 cut(s)	1647, 1743
Eco24I	GRGCYC	1 cut(s)	56
Eco47III	AGCGCT	1 cut(s)	1671
Eco57I	CTGAAG	3 cut(s)	138, 840, 1749
Eco88I	CYCGRG	1 cut(s)	960
Eco91I	GGTNACC	1 cut(s)	848
EcoNI	CCTNNNNNAGG	2 cut(s)	233, 1322
EcoO109I	RGGNCCY	1 cut(s)	53
EcoO65I	GGTNACC	1 cut(s)	848
EcoRII	CCWGG	2 cut(s)	294, 341
EcoT14I	CCWWGG	2 cut(s)	1647, 1743
EcoT22I	ATGCAT	1 cut(s)	930
EcoT38I	GRGCYC	1 cut(s)	56
ErhI	CCWWGG	2 cut(s)	1647, 1743
Esp3I	CGTCTC	1 cut(s)	1791
FaeI	CATG	4 cut(s)	320, 544, 1651, 1711
FaqI	GGGAC	2 cut(s)	10, 1849
FatI	CATG	4 cut(s)	316, 540, 1647, 1707
FauI	CCCGC	2 cut(s)	35, 1919
FauNDI	CATATG	1 cut(s)	1388
FblI	GTMKAC	1 cut(s)	1698
Fnu4HI	GCNGC	7 cut(s)	149, 289, 366, 1414, 1491, 1629, 1915
FokI	GGATG	5 cut(s)	227, 714, 1251, 1634, 1655
FriOI	GRGCYC	1 cut(s)	56
Fsp4HI	GCNGC	7 cut(s)	149, 289, 366, 1414, 1491, 1629, 1915
FspBI	CTAG	7 cut(s)	828, 894, 1161, 1191, 1238, 1275, 1352
GlaI	GCGC	2 cut(s)	92, 1671
GluI	GCNGC	7 cut(s)	149, 289, 366, 1414, 1491, 1629, 1915
GsuI	CTGGAG	3 cut(s)	293, 531, 1107
HaeII	RGCGCY	2 cut(s)	94, 1673
HaeIII	GGCC	4 cut(s)	54, 239, 584, 1385
HapII	CCGG	1 cut(s)	1640
HhaI	GCGC	2 cut(s)	93, 1672
Hin1II	CATG	4 cut(s)	320, 544, 1651, 1711
Hin6I	GCGC	2 cut(s)	91, 1670
HinP1I	GCGC	2 cut(s)	91, 1670
HincII	GTYRAC	2 cut(s)	438, 1876
HindII	GTYRAC	2 cut(s)	438, 1876
HpaII	CCGG	1 cut(s)	1640
HphI	GGTGA	4 cut(s)	522, 634, 842, 1440
Hpy166II	GTNNAC	5 cut(s)	438, 530, 1184, 1699, 1876
Hpy188III	TCNNGA	8 cut(s)	244, 386, 548, 704, 731, 1015, 1121, 1352
Hpy8I	GTNNAC	5 cut(s)	438, 530, 1184, 1699, 1876
Hpy99I	CGWCG	1 cut(s)	1872
HpyAV	CCTTC	7 cut(s)	85, 316, 701, 864, 1012, 1278, 1545
HpyCH4III	ACNGT	7 cut(s)	281, 337, 445, 537, 883, 1132, 1233
HpyCH4IV	ACGT	3 cut(s)	174, 763, 1784
HpyF10VI	GCNNNNNNNGC	4 cut(s)	97, 934, 982, 1778
HpyF3I	CTNAG	9 cut(s)	152, 197, 690, 695, 818, 1097, 1302, 1462, 1510
HpySE526I	ACGT	3 cut(s)	174, 763, 1784
Hsp92II	CATG	4 cut(s)	320, 544, 1651, 1711
HspAI	GCGC	2 cut(s)	91, 1670
KspI	CCGCGG	1 cut(s)	1915
Kzo9I	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
LmnI	GCTCC	7 cut(s)	550, 602, 1088, 1150, 1284, 1903, 1922
Lsp1109I	GCAGC	4 cut(s)	352, 1425, 1502, 1615
LweI	GCATC	6 cut(s)	565, 736, 1223, 1273, 1436, 1633
MaeI	CTAG	7 cut(s)	828, 894, 1161, 1191, 1238, 1275, 1352
MaeII	ACGT	3 cut(s)	174, 763, 1784
MaeIII	GTNAC	5 cut(s)	281, 622, 759, 848, 1233
MalI	GATC	8 cut(s)	6, 493, 744, 966, 981, 1119, 1198, 1584
MbiI	CCGCTC	1 cut(s)	151
MboI	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
MboII	GAAGA	8 cut(s)	253, 461, 478, 731, 793, 891, 1053, 1405
MfeI	CAATTG	1 cut(s)	359
MflI	RGATCY	3 cut(s)	491, 742, 964
MhlI	GDGCHC	1 cut(s)	56
MlsI	TGGCCA	1 cut(s)	1385
MluNI	TGGCCA	1 cut(s)	1385
MlyI	GAGTC	3 cut(s)	1016, 1349, 1871
MmeI	TCCRAC	3 cut(s)	580, 933, 1864
Mox20I	TGGCCA	1 cut(s)	1385
Mph1103I	ATGCAT	1 cut(s)	930
MscI	TGGCCA	1 cut(s)	1385
MseI	TTAA	2 cut(s)	413, 1814
MslI	CAYNNNNRTG	1 cut(s)	1296
Msp20I	TGGCCA	1 cut(s)	1385
MspA1I	CMGCKG	2 cut(s)	619, 1914
MspI	CCGG	1 cut(s)	1640
MspR9I	CCNGG	2 cut(s)	296, 343
MunI	CAATTG	1 cut(s)	359
MvaI	CCWGG	2 cut(s)	296, 343
MvnI	CGCG	2 cut(s)	80, 1914
MwoI	GCNNNNNNNGC	4 cut(s)	97, 934, 982, 1778
NcoI	CCATGG	1 cut(s)	1647
NdeI	CATATG	1 cut(s)	1388
NdeII	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
NlaIII	CATG	4 cut(s)	320, 544, 1651, 1711
NlaIV	GGNNCC	7 cut(s)	54, 55, 73, 101, 546, 1280, 1918
NmeAIII	GCCGAG	1 cut(s)	1901
NmuCI	GTSAC	3 cut(s)	281, 622, 848
NsiI	ATGCAT	1 cut(s)	930
PfeI	GAWTC	7 cut(s)	258, 824, 998, 1058, 1223, 1472, 1836
PflMI	CCANNNNNTGG	2 cut(s)	644, 1618
PkrI	GCNGC	7 cut(s)	150, 290, 367, 1415, 1492, 1630, 1916
PleI	GAGTC	3 cut(s)	1015, 1349, 1870
PpsI	GAGTC	3 cut(s)	1015, 1349, 1870
Psp6I	CCWGG	2 cut(s)	294, 341
PspEI	GGTNACC	1 cut(s)	848
PspGI	CCWGG	2 cut(s)	294, 341
PspN4I	GGNNCC	7 cut(s)	54, 55, 73, 101, 546, 1280, 1918
PspOMI	GGGCCC	1 cut(s)	52
PspPI	GGNCC	2 cut(s)	52, 53
PstI	CTGCAG	2 cut(s)	370, 752
PstNI	CAGNNNCTG	1 cut(s)	335
PsuI	RGATCY	3 cut(s)	491, 742, 964
PvuII	CAGCTG	1 cut(s)	619
RsaI	GTAC	7 cut(s)	115, 177, 278, 539, 1334, 1706, 1739
RsaNI	GTAC	7 cut(s)	114, 176, 277, 538, 1333, 1705, 1738
RseI	CAYNNNNRTG	1 cut(s)	1296
SacII	CCGCGG	1 cut(s)	1915
SaqAI	TTAA	2 cut(s)	413, 1814
SatI	GCNGC	7 cut(s)	149, 289, 366, 1414, 1491, 1629, 1915
Sau3AI	GATC	8 cut(s)	4, 491, 742, 964, 979, 1117, 1196, 1582
Sau96I	GGNCC	2 cut(s)	52, 53
ScaI	AGTACT	1 cut(s)	278
SchI	GAGTC	3 cut(s)	1016, 1349, 1871
ScrFI	CCNGG	2 cut(s)	296, 343
SduI	GDGCHC	1 cut(s)	56
SfaNI	GCATC	6 cut(s)	565, 736, 1223, 1273, 1436, 1633
SfcI	CTRYAG	3 cut(s)	366, 748, 1695
Sfr303I	CCGCGG	1 cut(s)	1915
SgrBI	CCGCGG	1 cut(s)	1915
SmiMI	CAYNNNNRTG	1 cut(s)	1296
SpeI	ACTAGT	1 cut(s)	1190
SsiI	CCGC	8 cut(s)	28, 80, 149, 288, 299, 419, 1912, 1914
SspI	AATATT	1 cut(s)	676
SspMI	CTAG	7 cut(s)	828, 894, 1161, 1191, 1238, 1275, 1352
StyD4I	CCNGG	2 cut(s)	294, 341
StyI	CCWWGG	2 cut(s)	1647, 1743
TaaI	ACNGT	7 cut(s)	281, 337, 445, 537, 883, 1132, 1233
TaiI	ACGT	3 cut(s)	177, 766, 1787
TaqI	TCGA	7 cut(s)	138, 234, 686, 885, 988, 1014, 1585
TaqII	GACCGA	1 cut(s)	263
TatI	WGTACW	5 cut(s)	276, 537, 1332, 1704, 1737
TauI	GCSGC	3 cut(s)	151, 291, 1917
TfiI	GAWTC	7 cut(s)	258, 824, 998, 1058, 1223, 1472, 1836
Tru1I	TTAA	2 cut(s)	413, 1814
Tru9I	TTAA	2 cut(s)	413, 1814
TscAI	CASTG	5 cut(s)	540, 864, 1105, 1236, 1729
TseFI	GTSAC	3 cut(s)	281, 622, 848
TseI	GCWGC	4 cut(s)	365, 1413, 1490, 1628
Tsp45I	GTSAC	3 cut(s)	281, 622, 848
TspDTI	ATGAA	6 cut(s)	392, 1064, 1485, 1485, 1527, 1558
TspGWI	ACGGA	2 cut(s)	809, 1794
TspRI	CASTG	5 cut(s)	540, 864, 1105, 1236, 1729
Van91I	CCANNNNNTGG	2 cut(s)	644, 1618
XagI	CCTNNNNNAGG	2 cut(s)	233, 1322
XapI	RAATTY	2 cut(s)	812, 1407
XbaI	TCTAGA	1 cut(s)	1351
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1619
XmiI	GTMKAC	1 cut(s)	1698
XspI	CTAG	7 cut(s)	828, 894, 1161, 1191, 1238, 1275, 1352
ZrmI	AGTACT	1 cut(s)	278
Zsp2I	ATGCAT	1 cut(s)	930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.