Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0010017

Physical Location & Seq

Genomic Coordinates Reverse (-)

21543619 .. 21547602

Gene Structure 3984 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1103300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGGCCACCGCTATACACCTACGCCGGCTCGGAGCCGCCGCCGTCGTTGCTACGGCAGCCAGCGGCGCCTTTCTCCTCCAGCCTTCGCTCTCCGCCAGCGACCGCGGCGCCGGGTCCTCCTCCCTCGGCGCTGTCCGCCAGAGGATCAGCGACCCGAATGCTGTCGTTCCGTCTAGGGCGGCTCAGGAGGCCGCTCTGATTGGCGCGAGTGCTGCCAACCCGCTCGACATTCTCGTGATTGGCGGTGGCGCCACCGGCTGCGGCGTCGCCCTTGACGCAACCACCAGAGGCCTCCGCGTCGGCCTCGTCGAGCGCGAGGACTTCTCTTCTGGAACGTCGTCGCGGTCCACTAAGCTAATTCATGGAGGTGTTCGGTACTTGGAGAAAGCTGTTTTTAATCTTGACTACGGGCAGCTGAAGTTGGTATTTCATGCGCTTGAGGAGCGTAAACAGGTTATTGACAATGCACCGCACCTCTGCCATGCTTTGCCATGCATGACACCATGTTTTGACTGGTTTGAGGTAGTATACTACTGGATGGGCTTGAAAATGTACGATTTGGTTGCAGCACTACGGCTCTTACATGTGTCCAGATATTATTCTGCACAAGAATCTGTTGAGCTCTTCCCCACCCTTGCCAGGAAGGGTAACAATAAAAGCTTGAAGGGCACCGTGGTGTATTATGATGGGCAGATGAATGACGCGCGTCTTAATGTTGGATTGGCATGCACTGCTGCCTTAGCTGGTGCAGCGGTGCTTAACCATGCAGAAGTGGTTGATTTGTTGAAGGATGAGGCTAGCAATCGGACAATTGGTGCAAGAATCCGAGACAACTTATCAGGGAAAGAGTTTAATACATATGCAAAAGTTGTCGTGAATGCTGCTGGGCCATTTTGTGACTCTTTAAGGAAGATGACAGATCAAGATGCAAAACCAATGATATGCCCAAGCAGCGGTGTACACATCGTACTTCCTGACTATTACTCTCCTGAGGGTATGGGTTTGATTGTTCCTAAAACTAAGGATGGACGTGTTGTTTTCATGCTTCCTTGGTTGGGACGAACAATTGCTGGCACCACAGATTCCAACACCCCTATTACTCTGCTTCCTGAACCACATGAGGATGAGATTCAATTCATACTGGATGCCATTTGTGATTACCTTAATGTCAAGGTACGCCGTACGGATGTTCTCTCCGCGTGGAGTGGTATTCGTCCATTGGCAACGGATCCATCTGCGAAAAGCACGGAGAGCATCTCCAGAGATCATGTTGTATGTGAAGATTATCCTGGTTTGGTAACAATCACGGGCGGCAAGTGGACTACTTACCGTGGCATGGCAGAAGATGCGGTTAATGCAGCAATAAAGTCTGGAAAGCTGACCCCAGAAAATGGATGTTTAACCTCCAAGCTGCAAATTGTCGGTGGAGACGGATGGGATCCTGCATCTTTTACAGTTATCGCTCAACAATATGTGCGCATGAAGAACAGCCATGGTAAAGTTGTTCCTGGAGTGATGGACACTGCTGCAGCAAAGCATCTATCCCATGCATATGGTACTTTGGCTGAACGAGTTGCTGCTATTGCTCAGAACGAAAATCTGGGGAAGAGGCTTGCCCATGGATACCCTTTTCTAGAGGCTGAGGTTGCCTACTGTGCCCGAAACGAGTACTGTGAATCTGCCATTGATTTCATTGCTCGGAGATCTCGGCTTGCTTTCCTCGACACTGATGCAGCAAGCAGGGCATTGCCACGTGTGATTGAAATTTTAGCGACCGAACACAACTGGGATGATTCGAGGCAGAAGTACGAGTTAGAGAAGGCCACGGAATTTTTGAAAACTTTCAAATCTTCAAAAAATGCTCAGTTCCATGACGGGAAACATGTTTGTGCATTCATTTTTCACAGGTACATTGACCGGCGGCATATTTTCCGGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004368 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006066 GO:0006071 GO:0006072 GO:0006091 GO:0006116 GO:0006127 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009117 GO:0009987 GO:0015980 GO:0016052 GO:0016491 GO:0016614 GO:0016901 GO:0019362 GO:0019400 GO:0019405 GO:0019563 GO:0019637 GO:0019674 GO:0019751 GO:0022900 GO:0022904 GO:0034308 GO:0034641 GO:0042180 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044262 GO:0044275 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046164 GO:0046174 GO:0046483 GO:0046496 GO:0051186 GO:0052646 GO:0055086 GO:0055114 GO:0071704 GO:0072524 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1901615 GO:1901616

KEGG Pathways

Metabolic & Signaling

ko00564 ko01110 map00564 map01110

Pfam Domains

Protein Families

DAO DAO_C

Protein Analysis

647

Amino Acids

70.38

Weight (kDa)

8.12

Isoelectric Point (pI)

38.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAO	PF01266	76 - 444	5.7e-57	FAD dependent oxidoreductase
DAO_C	PF16901	466 - 600	5.5e-37	C-terminal domain of alpha-glycerophosphate oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010017)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10370
fragaria_vesca	FvH4_7g18820 FvH4_7g18820 FvH4_7g18820 FvH4_7g18820 FvH4_7g18820
malus_domestica	MD01G1103300.v1.1 MD07G1170100.v1.1 MD07G1170200.v1.1
prunus_persica	Prupe.2G209000_v2.0.a1
pyrus_communis	pycom01g12980
rosa_chinensis	RchiOBHm_Chr1g0362611
rosa_laevigata	RLG00000027634
rosa_multiflora	Rmu_sc0005725.1_g000012
rosa_roxburghii	Rroxscaffold_4G00293900
rosa_rugosa	Rorug01G0300100.1
rosa_samantha	Rh1AG308700 Rh1BG272400 Rh1CG289600 Rh1DG302800
rosa_wichuraiana	Rw1G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1478
AccB1I	GGYRCC	5 cut(s)	65, 107, 248, 668, 1073
AccB7I	CCANNNNNTGG	1 cut(s)	1391
AccBSI	CCGCTC	2 cut(s)	194, 223
AccI	GTMKAC	1 cut(s)	528
AccII	CGCG	8 cut(s)	105, 206, 297, 315, 343, 704, 706, 1199
AclWI	GGATC	5 cut(s)	152, 1223, 1236, 1433, 1446
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	1764, 1829
AcuI	CTGAAG	1 cut(s)	437
AcvI	CACGTG	1 cut(s)	1754
AcyI	GRCGYC	4 cut(s)	66, 108, 249, 264
AfiI	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
AflIII	ACRYGT	4 cut(s)	583, 1030, 1753, 1882
AgsI	TTSAA	8 cut(s)	547, 664, 787, 1133, 1763, 1837, 1846, 1854
AjiI	CACGTC	1 cut(s)	1031
AjnI	CCWGG	3 cut(s)	638, 1288, 1507
AleI	CACNNNNGTG	1 cut(s)	674
AluBI	AGCT	8 cut(s)	355, 389, 415, 622, 660, 743, 1378, 1411
AluI	AGCT	8 cut(s)	355, 389, 415, 622, 660, 743, 1378, 1411
Alw21I	GWGCWC	1 cut(s)	624
Alw26I	GTCTC	2 cut(s)	822, 1422
AlwI	GGATC	5 cut(s)	152, 1223, 1236, 1433, 1446
AoxI	GGCC	7 cut(s)	3, 189, 289, 301, 887, 1821, 1934
ApoI	RAATTY	2 cut(s)	1764, 1829
Asp700I	GAANNNNTTC	1 cut(s)	1841
AspLEI	GCGC	9 cut(s)	68, 110, 131, 206, 251, 315, 436, 706, 1479
AspS9I	GGNCC	3 cut(s)	114, 345, 887
AsuC2I	CCSGG	1 cut(s)	112
AsuNHI	GCTAGC	1 cut(s)	797
AvaII	GGWCC	2 cut(s)	114, 345
AxyI	CCTNAGG	1 cut(s)	990
BaeGI	GKGCMC	2 cut(s)	671, 1660
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	2 cut(s)	1228, 1438
BanI	GGYRCC	5 cut(s)	65, 107, 248, 668, 1073
BanII	GRGCYC	1 cut(s)	624
BauI	CACGAG	1 cut(s)	233
BbrPI	CACGTG	1 cut(s)	1754
Bbv12I	GWGCWC	1 cut(s)	624
BbvCI	CCTCAGC	1 cut(s)	1641
BccI	CCATC	6 cut(s)	532, 680, 1019, 1240, 1428, 1510
BceAI	ACGGC	4 cut(s)	26, 69, 590, 1164
BcgI	CGANNNNNNTGC	6 cut(s)	140, 174, 545, 579, 1712, 1746
BciT130I	CCWGG	3 cut(s)	640, 1290, 1509
BciVI	GTATCC	1 cut(s)	1616
BcnI	CCSGG	1 cut(s)	112
BcoDI	GTCTC	2 cut(s)	822, 1422
BfaI	CTAG	3 cut(s)	174, 798, 1634
BfmI	CTRYAG	1 cut(s)	1527
BfoI	RGCGCY	4 cut(s)	69, 111, 132, 252
BfuI	GTATCC	1 cut(s)	1616
BglII	AGATCT	1 cut(s)	1703
BmcAI	AGTACT	1 cut(s)	1670
Bme1390I	CCNGG	4 cut(s)	112, 640, 1290, 1509
Bme18I	GGWCC	2 cut(s)	114, 345
BmgBI	CACGTC	1 cut(s)	1031
BmgT120I	GGNCC	3 cut(s)	114, 345, 887
BmiI	GGNNCC	9 cut(s)	34, 67, 109, 115, 250, 670, 1075, 1230, 1440
BmrFI	CCNGG	4 cut(s)	112, 640, 1290, 1509
BmrI	ACTGGG	1 cut(s)	1795
BmsI	GCATC	7 cut(s)	916, 1135, 1263, 1336, 1454, 1546, 1720
BmtI	GCTAGC	1 cut(s)	801
BmuI	ACTGGG	1 cut(s)	1795
BoxI	GACNNNNGTC	1 cut(s)	705
BplI	GAGNNNNNCTC	4 cut(s)	308, 340, 1241, 1273
BpmI	CTGGAG	3 cut(s)	62, 1243, 1530
Bpu10I	CCTNAGC	3 cut(s)	183, 739, 1641
BpuEI	CTTGAG	1 cut(s)	458
BpuMI	CCSGG	1 cut(s)	112
BsaAI	YACGTR	1 cut(s)	1754
BsaHI	GRCGYC	4 cut(s)	66, 108, 249, 264
BsaJI	CCNNGG	8 cut(s)	103, 124, 672, 1049, 1330, 1492, 1618, 1824
Bsc4I	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
Bse118I	RCCGGY	3 cut(s)	24, 254, 1917
Bse1I	ACTGG	4 cut(s)	518, 539, 1146, 1790
Bse21I	CCTNAGG	1 cut(s)	990
Bse3DI	GCAATG	2 cut(s)	1692, 1745
BseBI	CCWGG	3 cut(s)	640, 1290, 1509
BseDI	CCNNGG	8 cut(s)	103, 124, 672, 1049, 1330, 1492, 1618, 1824
BseGI	GGATG	9 cut(s)	543, 796, 1030, 1129, 1150, 1192, 1400, 1439, 1795
BseLI	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
BseMI	GCAATG	2 cut(s)	1692, 1745
BseMII	CTCAG	5 cut(s)	197, 981, 1601, 1632, 1877
BseNI	ACTGG	4 cut(s)	518, 539, 1146, 1790
BseRI	GAGGAG	3 cut(s)	65, 109, 455
BseSI	GKGCMC	2 cut(s)	671, 1660
BseYI	CCCAGC	1 cut(s)	884
BsgI	GTGCAG	2 cut(s)	588, 768
Bsh1236I	CGCG	8 cut(s)	105, 206, 297, 315, 343, 704, 706, 1199
Bsh1285I	CGRYCG	2 cut(s)	103, 1776
BshFI	GGCC	7 cut(s)	5, 191, 291, 303, 889, 1823, 1936
BshNI	GGYRCC	5 cut(s)	65, 107, 248, 668, 1073
BsiEI	CGRYCG	2 cut(s)	103, 1776
BsiHKAI	GWGCWC	1 cut(s)	624
BsiSI	CCGG	5 cut(s)	25, 111, 255, 1918, 1933
BsiWI	CGTACG	1 cut(s)	1181
BslFI	GGGAC	1 cut(s)	1071
BslI	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
BsmAI	GTCTC	2 cut(s)	822, 1422
BsmBI	CGTCTC	1 cut(s)	1422
BsmFI	GGGAC	1 cut(s)	1071
BsmI	GAATGC	3 cut(s)	163, 883, 1892
BsnI	GGCC	7 cut(s)	5, 191, 291, 303, 889, 1823, 1936
Bsp1286I	GDGCHC	3 cut(s)	624, 671, 1660
Bsp1407I	TGTACA	1 cut(s)	958
Bsp143I	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
Bsp19I	CCATGG	2 cut(s)	1492, 1618
BspANI	GGCC	7 cut(s)	5, 191, 291, 303, 889, 1823, 1936
BspCNI	CTCAG	5 cut(s)	196, 982, 1600, 1633, 1876
BspFNI	CGCG	8 cut(s)	105, 206, 297, 315, 343, 704, 706, 1199
BspLI	GGNNCC	9 cut(s)	34, 67, 109, 115, 250, 670, 1075, 1230, 1440
BspMAI	CTGCAG	1 cut(s)	1531
BspOI	GCTAGC	1 cut(s)	801
BspPI	GGATC	5 cut(s)	152, 1223, 1236, 1433, 1446
BspQI	GCTCTTC	1 cut(s)	629
BspT107I	GGYRCC	5 cut(s)	65, 107, 248, 668, 1073
BsrBI	CCGCTC	2 cut(s)	194, 223
BsrDI	GCAATG	2 cut(s)	1692, 1745
BsrFI	RCCGGY	3 cut(s)	24, 254, 1917
BsrGI	TGTACA	1 cut(s)	958
BsrI	ACTGG	4 cut(s)	518, 539, 1146, 1790
BssAI	RCCGGY	3 cut(s)	24, 254, 1917
BssECI	CCNNGG	8 cut(s)	103, 124, 672, 1049, 1330, 1492, 1618, 1824
BssMI	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
BssNAI	GTATAC	1 cut(s)	529
BssNI	GRCGYC	4 cut(s)	66, 108, 249, 264
BssSI	CACGAG	1 cut(s)	233
BssT1I	CCWWGG	3 cut(s)	1049, 1492, 1618
Bst1107I	GTATAC	1 cut(s)	529
Bst2BI	CACGAG	1 cut(s)	233
Bst2UI	CCWGG	3 cut(s)	640, 1290, 1509
Bst4CI	ACNGT	5 cut(s)	673, 1331, 1456, 1655, 1673
Bst6I	CTCTTC	3 cut(s)	331, 629, 1601
BstACI	GRCGYC	4 cut(s)	66, 108, 249, 264
BstAPI	GCANNNNNTGC	2 cut(s)	731, 1346
BstAUI	TGTACA	1 cut(s)	958
BstBAI	YACGTR	1 cut(s)	1754
BstC8I	GCNNGC	9 cut(s)	26, 61, 97, 727, 799, 1072, 1614, 1713, 1738
BstDEI	CTNAG	8 cut(s)	183, 351, 739, 990, 1020, 1587, 1641, 1863
BstDSI	CCRYGG	6 cut(s)	103, 672, 1330, 1492, 1618, 1824
BstF5I	GGATG	9 cut(s)	543, 796, 1030, 1129, 1150, 1192, 1400, 1439, 1795
BstFNI	CGCG	8 cut(s)	105, 206, 297, 315, 343, 704, 706, 1199
BstH2I	RGCGCY	4 cut(s)	69, 111, 132, 252
BstHHI	GCGC	9 cut(s)	68, 110, 131, 206, 251, 315, 436, 706, 1479
BstKTI	GATC	6 cut(s)	147, 922, 1231, 1267, 1441, 1706
BstMAI	GTCTC	2 cut(s)	822, 1422
BstMBI	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
BstMCI	CGRYCG	2 cut(s)	103, 1776
BstNI	CCWGG	3 cut(s)	640, 1290, 1509
BstNSI	RCATGY	3 cut(s)	587, 729, 1886
BstPAI	GACNNNNGTC	1 cut(s)	705
BstSCI	CCNGG	4 cut(s)	110, 638, 1288, 1507
BstSFI	CTRYAG	1 cut(s)	1527
BstSLI	GKGCMC	2 cut(s)	671, 1660
BstUI	CGCG	8 cut(s)	105, 206, 297, 315, 343, 704, 706, 1199
BstX2I	RGATCY	3 cut(s)	1228, 1438, 1703
BstXI	CCANNNNNNTGG	1 cut(s)	1553
BstYI	RGATCY	3 cut(s)	1228, 1438, 1703
BstZ17I	GTATAC	1 cut(s)	529
Bsu36I	CCTNAGG	1 cut(s)	990
BsuI	GTATCC	1 cut(s)	1616
BsuRI	GGCC	7 cut(s)	5, 191, 291, 303, 889, 1823, 1936
BtgI	CCRYGG	6 cut(s)	103, 672, 1330, 1492, 1618, 1824
BtrI	CACGTC	1 cut(s)	1031
BtsCI	GGATG	9 cut(s)	543, 796, 1030, 1129, 1150, 1192, 1400, 1439, 1795
BtsI	GCAGTG	2 cut(s)	729, 1521
BtsIMutI	CAGTG	3 cut(s)	729, 1521, 1725
Cac8I	GCNNGC	9 cut(s)	26, 61, 97, 727, 799, 1072, 1614, 1713, 1738
CfoI	GCGC	9 cut(s)	68, 110, 131, 206, 251, 315, 436, 706, 1479
Cfr10I	RCCGGY	3 cut(s)	24, 254, 1917
Cfr13I	GGNCC	3 cut(s)	114, 345, 887
Cfr42I	CCGCGG	1 cut(s)	106
CseI	GACGC	5 cut(s)	253, 284, 286, 695, 710
DdeI	CTNAG	8 cut(s)	183, 351, 739, 990, 1020, 1587, 1641, 1863
DinI	GGCGCC	3 cut(s)	67, 109, 250
DpnI	GATC	6 cut(s)	146, 921, 1230, 1266, 1440, 1705
DpnII	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	331, 629, 1601
EarI	CTCTTC	3 cut(s)	331, 629, 1601
EciI	GGCGGA	2 cut(s)	82, 125
Ecl136II	GAGCTC	1 cut(s)	622
Eco130I	CCWWGG	3 cut(s)	1049, 1492, 1618
Eco147I	AGGCCT	1 cut(s)	291
Eco24I	GRGCYC	1 cut(s)	624
Eco47I	GGWCC	2 cut(s)	114, 345
Eco53kI	GAGCTC	1 cut(s)	622
Eco57I	CTGAAG	1 cut(s)	437
Eco72I	CACGTG	1 cut(s)	1754
Eco81I	CCTNAGG	1 cut(s)	990
EcoICRI	GAGCTC	1 cut(s)	622
EcoO109I	RGGNCCY	1 cut(s)	114
EcoRII	CCWGG	3 cut(s)	638, 1288, 1507
EcoT14I	CCWWGG	3 cut(s)	1049, 1492, 1618
EcoT22I	ATGCAT	2 cut(s)	497, 1552
EcoT38I	GRGCYC	1 cut(s)	624
EgeI	GGCGCC	3 cut(s)	67, 109, 250
EheI	GGCGCC	3 cut(s)	67, 109, 250
ErhI	CCWWGG	3 cut(s)	1049, 1492, 1618
Esp3I	CGTCTC	1 cut(s)	1422
FaqI	GGGAC	1 cut(s)	1071
FauI	CCCGC	1 cut(s)	228
FauNDI	CATATG	2 cut(s)	859, 1552
FblI	GTMKAC	1 cut(s)	528
FokI	GGATG	9 cut(s)	550, 803, 1037, 1136, 1157, 1199, 1407, 1446, 1802
FriOI	GRGCYC	1 cut(s)	624
FspAI	RTGCGCAY	1 cut(s)	1478
FspBI	CTAG	3 cut(s)	174, 798, 1634
FspI	TGCGCA	1 cut(s)	1478
GlaI	GCGC	9 cut(s)	67, 109, 130, 205, 250, 314, 435, 705, 1478
GsaI	CCCAGC	1 cut(s)	888
GsuI	CTGGAG	3 cut(s)	62, 1243, 1530
HaeII	RGCGCY	4 cut(s)	69, 111, 132, 252
HaeIII	GGCC	7 cut(s)	5, 191, 291, 303, 889, 1823, 1936
HapII	CCGG	5 cut(s)	25, 111, 255, 1918, 1933
HgaI	GACGC	5 cut(s)	253, 284, 286, 695, 710
HhaI	GCGC	9 cut(s)	68, 110, 131, 206, 251, 315, 436, 706, 1479
Hin1I	GRCGYC	4 cut(s)	66, 108, 249, 264
Hin6I	GCGC	9 cut(s)	66, 108, 129, 204, 249, 313, 434, 704, 1477
HinP1I	GCGC	9 cut(s)	66, 108, 129, 204, 249, 313, 434, 704, 1477
HindIII	AAGCTT	1 cut(s)	658
HinfI	GANTC	7 cut(s)	611, 822, 899, 1082, 1129, 1676, 1793
HpaII	CCGG	5 cut(s)	25, 111, 255, 1918, 1933
Hpy166II	GTNNAC	6 cut(s)	348, 449, 529, 959, 961, 1320
Hpy188I	TCNGA	6 cut(s)	32, 198, 807, 827, 1590, 1701
Hpy8I	GTNNAC	6 cut(s)	348, 449, 529, 959, 961, 1320
Hpy99I	CGWCG	6 cut(s)	47, 269, 302, 311, 340, 343
HpyAV	CCTTC	5 cut(s)	93, 637, 658, 781, 1813
HpyCH4III	ACNGT	5 cut(s)	673, 1331, 1456, 1655, 1673
HpyCH4IV	ACGT	3 cut(s)	335, 1030, 1753
HpyF3I	CTNAG	8 cut(s)	183, 351, 739, 990, 1020, 1587, 1641, 1863
HpySE526I	ACGT	3 cut(s)	335, 1030, 1753
Hsp92I	GRCGYC	4 cut(s)	66, 108, 249, 264
HspAI	GCGC	9 cut(s)	66, 108, 129, 204, 249, 313, 434, 704, 1477
KasI	GGCGCC	3 cut(s)	65, 107, 248
KroI	GCCGGC	1 cut(s)	24
KroNI	GCCGGC	1 cut(s)	26
KspI	CCGCGG	1 cut(s)	106
Kzo9I	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
LguI	GCTCTTC	1 cut(s)	629
LmnI	GCTCC	2 cut(s)	32, 442
LweI	GCATC	7 cut(s)	916, 1135, 1263, 1336, 1454, 1546, 1720
MaeI	CTAG	3 cut(s)	174, 798, 1634
MaeII	ACGT	3 cut(s)	335, 1030, 1753
MaeIII	GTNAC	3 cut(s)	647, 896, 1297
MalI	GATC	6 cut(s)	146, 921, 1230, 1266, 1440, 1705
MbiI	CCGCTC	2 cut(s)	194, 223
MboI	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
MboII	GAAGA	8 cut(s)	318, 616, 922, 1292, 1355, 1495, 1618, 1842
MfeI	CAATTG	2 cut(s)	810, 1065
MflI	RGATCY	3 cut(s)	1228, 1438, 1703
MhlI	GDGCHC	3 cut(s)	624, 671, 1660
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	7 cut(s)	357, 810, 1065, 1133, 1416, 1764, 1829
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	3 cut(s)	66, 108, 249
MlyI	GAGTC	1 cut(s)	893
MmeI	TCCRAC	2 cut(s)	697, 1110
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	497, 1552
MroNI	GCCGGC	1 cut(s)	24
MroXI	GAANNNNTTC	1 cut(s)	1841
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	9 cut(s)	396, 711, 759, 852, 905, 1164, 1353, 1400, 1939
MslI	CAYNNNNRTG	6 cut(s)	233, 366, 674, 1122, 1551, 1887
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	5 cut(s)	63, 105, 415, 752, 954
MspI	CCGG	5 cut(s)	25, 111, 255, 1918, 1933
MspR9I	CCNGG	4 cut(s)	112, 640, 1290, 1509
MunI	CAATTG	2 cut(s)	810, 1065
Mva1269I	GAATGC	3 cut(s)	163, 883, 1892
MvaI	CCWGG	3 cut(s)	640, 1290, 1509
MvnI	CGCG	8 cut(s)	105, 206, 297, 315, 343, 704, 706, 1199
NaeI	GCCGGC	1 cut(s)	26
NarI	GGCGCC	3 cut(s)	66, 108, 249
NciI	CCSGG	1 cut(s)	112
NcoI	CCATGG	2 cut(s)	1492, 1618
NdeI	CATATG	2 cut(s)	859, 1552
NdeII	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
NgoMIV	GCCGGC	1 cut(s)	24
NheI	GCTAGC	1 cut(s)	797
NlaIV	GGNNCC	9 cut(s)	34, 67, 109, 115, 250, 670, 1075, 1230, 1440
NmeAIII	GCCGAG	2 cut(s)	105, 1687
NmuCI	GTSAC	1 cut(s)	896
NsbI	TGCGCA	1 cut(s)	1478
NsiI	ATGCAT	2 cut(s)	497, 1552
NspI	RCATGY	3 cut(s)	587, 729, 1886
OliI	CACNNNNGTG	1 cut(s)	674
PaeI	GCATGC	1 cut(s)	729
PceI	AGGCCT	1 cut(s)	291
PciI	ACATGT	2 cut(s)	583, 1882
PciSI	GCTCTTC	1 cut(s)	629
PcsI	WCGNNNNNNNCGW	3 cut(s)	231, 306, 312
PctI	GAATGC	3 cut(s)	163, 883, 1892
PdiI	GCCGGC	1 cut(s)	26
PdmI	GAANNNNTTC	1 cut(s)	1841
PfeI	GAWTC	6 cut(s)	611, 822, 1082, 1129, 1676, 1793
Pfl23II	CGTACG	1 cut(s)	1181
PflMI	CCANNNNNTGG	1 cut(s)	1391
PfoI	TCCNGGA	1 cut(s)	1507
PleI	GAGTC	1 cut(s)	893
PluTI	GGCGCC	3 cut(s)	69, 111, 252
PmaCI	CACGTG	1 cut(s)	1754
PmlI	CACGTG	1 cut(s)	1754
PpsI	GAGTC	1 cut(s)	893
Ppu21I	YACGTR	1 cut(s)	1754
PpuMI	RGGWCCY	1 cut(s)	114
PscI	ACATGT	2 cut(s)	583, 1882
PshAI	GACNNNNGTC	1 cut(s)	705
Psp124BI	GAGCTC	1 cut(s)	624
Psp5II	RGGWCCY	1 cut(s)	114
Psp6I	CCWGG	3 cut(s)	638, 1288, 1507
PspCI	CACGTG	1 cut(s)	1754
PspFI	CCCAGC	1 cut(s)	884
PspGI	CCWGG	3 cut(s)	638, 1288, 1507
PspLI	CGTACG	1 cut(s)	1181
PspN4I	GGNNCC	9 cut(s)	34, 67, 109, 115, 250, 670, 1075, 1230, 1440
PspPI	GGNCC	3 cut(s)	114, 345, 887
PspPPI	RGGWCCY	1 cut(s)	114
PstI	CTGCAG	1 cut(s)	1531
PsuI	RGATCY	3 cut(s)	1228, 1438, 1703
PvuII	CAGCTG	1 cut(s)	415
RseI	CAYNNNNRTG	6 cut(s)	233, 366, 674, 1122, 1551, 1887
SacI	GAGCTC	1 cut(s)	624
SacII	CCGCGG	1 cut(s)	106
SapI	GCTCTTC	1 cut(s)	629
SaqAI	TTAA	9 cut(s)	396, 711, 759, 852, 905, 1164, 1353, 1400, 1939
Sau3AI	GATC	6 cut(s)	144, 919, 1228, 1264, 1438, 1703
Sau96I	GGNCC	3 cut(s)	114, 345, 887
ScaI	AGTACT	1 cut(s)	1670
SchI	GAGTC	1 cut(s)	893
ScrFI	CCNGG	4 cut(s)	112, 640, 1290, 1509
SduI	GDGCHC	3 cut(s)	624, 671, 1660
SfaNI	GCATC	7 cut(s)	916, 1135, 1263, 1336, 1454, 1546, 1720
SfcI	CTRYAG	1 cut(s)	1527
SfoI	GGCGCC	3 cut(s)	67, 109, 250
Sfr303I	CCGCGG	1 cut(s)	106
SgrBI	CCGCGG	1 cut(s)	106
SinI	GGWCC	2 cut(s)	114, 345
SmiMI	CAYNNNNRTG	6 cut(s)	233, 366, 674, 1122, 1551, 1887
SmlI	CTYRAG	1 cut(s)	437
SmoI	CTYRAG	1 cut(s)	437
SphI	GCATGC	1 cut(s)	729
Sse9I	AATT	7 cut(s)	357, 810, 1065, 1133, 1416, 1764, 1829
SseBI	AGGCCT	1 cut(s)	291
SspDI	GGCGCC	3 cut(s)	65, 107, 248
SspMI	CTAG	3 cut(s)	174, 798, 1634
SstI	GAGCTC	1 cut(s)	624
StuI	AGGCCT	1 cut(s)	291
StyD4I	CCNGG	4 cut(s)	110, 638, 1288, 1507
StyI	CCWWGG	3 cut(s)	1049, 1492, 1618
TaaI	ACNGT	5 cut(s)	673, 1331, 1456, 1655, 1673
TaiI	ACGT	3 cut(s)	338, 1033, 1756
TaqI	TCGA	4 cut(s)	225, 309, 1722, 1796
TaqII	GACCGA	1 cut(s)	1790
TasI	AATT	7 cut(s)	357, 810, 1065, 1133, 1416, 1764, 1829
TatI	WGTACW	2 cut(s)	958, 1668
TauI	GCSGC	9 cut(s)	38, 41, 66, 108, 182, 194, 264, 1314, 1924
TfiI	GAWTC	6 cut(s)	611, 822, 1082, 1129, 1676, 1793
Tru1I	TTAA	9 cut(s)	396, 711, 759, 852, 905, 1164, 1353, 1400, 1939
Tru9I	TTAA	9 cut(s)	396, 711, 759, 852, 905, 1164, 1353, 1400, 1939
TscAI	CASTG	3 cut(s)	736, 1528, 1732
TseFI	GTSAC	1 cut(s)	896
Tsp45I	GTSAC	1 cut(s)	896
TspDTI	ATGAA	8 cut(s)	350, 419, 710, 1030, 1126, 1496, 1681, 1885
TspGWI	ACGGA	6 cut(s)	159, 1199, 1241, 1262, 1446, 1841
TspRI	CASTG	3 cut(s)	736, 1528, 1732
Van91I	CCANNNNNTGG	1 cut(s)	1391
VpaK11BI	GGWCC	2 cut(s)	114, 345
XapI	RAATTY	2 cut(s)	1764, 1829
XbaI	TCTAGA	1 cut(s)	1633
XceI	RCATGY	3 cut(s)	587, 729, 1886
XcmI	CCANNNNNNNNNTGG	1 cut(s)	510
XmiI	GTMKAC	1 cut(s)	528
XmnI	GAANNNNTTC	1 cut(s)	1841
XspI	CTAG	3 cut(s)	174, 798, 1634
ZrmI	AGTACT	1 cut(s)	1670
Zsp2I	ATGCAT	2 cut(s)	497, 1552

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.