Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010017

Physical Location & Seq

Genomic Coordinates Reverse (-)

24496311 .. 24499753

Gene Structure 3443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1170200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGCCACCGCCACACGCCTGCGTCGGCTCGGAGCTGCCGCCTGCGTCGCCACCGCGGCAAGCGGCGCCTTTCTCCTCCAGCCTTCCTTCTCCGCCAACGACCGCGGCAATGGACCCTCCTCCCTCGGCGCCGTTCGCCAGAGGATCAGCGACCCGAACGCTGTCGTTCCGTCTAGAGCGGCTCAGGAGGCCGCTCTGATTAGCGCGAGCGCTTCCAACCCGCTCGACATTCTCGTCATCGGCGGTGGCGCTACCGGAAGTGGCGTCGCTCTTGACGCCGTCACCAGAGGCCTCCGCATCGGCCTCGTCGAGCGCGAGGACTTCTCTTCTGGAACGTCGTCGCGGTCCACCAAGCTAATTCATGGAGGTGTTCGGTACTTGGAGAAAGCCGTGTTTAATCTTGACTATGGACAGCTGAAGTTGGTATTCCATGCGCTTGAGGAGCGTAAACAGGTTATTGAGAATGCACCACACCTCTGTCATGCTTTGCCATGCATGACACCGTGTTTTGACTGGTTTGAGGTAGTGTACTACTGGATGGGCTTGAAAATGTACGATTTGGTGGCAGGACTACGACTCTTACATGTGTCCAGATATTATTCGGCACAAGAATCTGTTGAGCTGTTCCCCACCCTTGCCAGGAAGGGTAACAATAAAAGCTTGAAGGGCACCGTGGTGTATTATGATGGGCAGATGAATGACGCACGTCTTAATGTTGGATTGGCGTGCACTGCTGCCGTAGCTGGTGCAGCAGTGCTTAACCATGCAGAAGTGGTTGCTTTGTTGAAGGATGAGGCTAGCAATCGGACAATTGGTGCAAGAATCCGAGACAACTTATCAGGGAAAGAGTTTGATATATATGCAAAAGTCGTCGTGAATGCTGCCGGGCCATTTTGTGACTCTTTAAGGAAGATGGCAGATCAAGATGCAAAACCAATGATATGCCCAAGCAGCGGTGTACATATCGTACTTCCTGACTATTACTCTCCTGAGGGTATGGGTTTGATTGTTCCTAAAACTAAGGATGGACGTGTTGTTTTCATGCTTCCATGGTTGGGACGAACAATTGCTGGCACCACAGATTCCAACACCTCTATTACTCTTCTTCCTGAACCACATGAGGATGAGATTCAGTTCATACTGGATGCCATTTGTGATTACCTTAATGTCAAGGTACGCCGCACAGACGTTCTCTCTGCGTGGAGTGGTATTCGGCCATTGGCAACGGATCCATCTGCGAAAAGCACTGAGAGCATCTCCAGAGATCATGTTGTATGTGAAGATTATCCTGGTTTGGTAACAATCACGGGCGGCAAGTGGACTACTTACCGTGGCATGGCAGAAGATGCGGTTAATGCAGCAATAAAGTCTGGAAATCTGACCCCAGAAAATGGATGTTTGACCTCCAAGCTTCAGATCGTCGGTGGAGACGGATGGGACCCTGCATCTTTTACAGTTATAGCTCAACAATATGTGCGCATGAAGAACAGCCATGGTAAAGTTGTTCCTGCAGTGATGGACACTGCTGCAGCAAGGCATCTGTCCCATGCATATGGTACTTTTGCTGAACGAGTTGCTGCCATTGCTCAGAACGAAAATCTGGGGAAGAGGCTTGCCCATGGATACCCCTTTCTAGAGGCTGAGGTTGCCTACTGTGCTCGAAATGAGTACTGTGAATCTGCCATTGATTTCATTGCTAGGAGATCTCGGCTTGCTTTCCTCGACACTGATGCGGCAAGCAAGGCATTGCCACGTGTGATTGAAATTTTAGCAACTGAACACAAGTGGGACAATTCTAGGCAGAAGTACGAGTTAGAGAAAGCCAAGGAATTTTTGGAAACTTTCAAATCTTTCAAAAAATGCTCAGTTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004368 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0006066 GO:0006071 GO:0006072 GO:0006091 GO:0006116 GO:0006127 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009117 GO:0009987 GO:0015980 GO:0016052 GO:0016491 GO:0016614 GO:0016901 GO:0019362 GO:0019400 GO:0019405 GO:0019563 GO:0019637 GO:0019674 GO:0019751 GO:0022900 GO:0022904 GO:0034308 GO:0034641 GO:0042180 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044262 GO:0044275 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046164 GO:0046174 GO:0046483 GO:0046496 GO:0051186 GO:0052646 GO:0055086 GO:0055114 GO:0071704 GO:0072524 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1901615 GO:1901616

KEGG Pathways

Metabolic & Signaling

ko00564 ko01110 map00564 map01110

Pfam Domains

Protein Families

DAO DAO_C

Protein Analysis

625

Amino Acids

67.75

Weight (kDa)

7.88

Isoelectric Point (pI)

39.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAO	PF01266	76 - 444	8.4e-56	FAD dependent oxidoreductase
DAO_C	PF16901	466 - 600	2.9e-36	C-terminal domain of alpha-glycerophosphate oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010017)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10370
fragaria_vesca	FvH4_7g18820 FvH4_7g18820 FvH4_7g18820 FvH4_7g18820 FvH4_7g18820
malus_domestica	MD01G1103300.v1.1 MD07G1170100.v1.1 MD07G1170200.v1.1
prunus_persica	Prupe.2G209000_v2.0.a1
pyrus_communis	pycom01g12980
rosa_chinensis	RchiOBHm_Chr1g0362611
rosa_laevigata	RLG00000027634
rosa_multiflora	Rmu_sc0005725.1_g000012
rosa_roxburghii	Rroxscaffold_4G00293900
rosa_rugosa	Rorug01G0300100.1
rosa_samantha	Rh1AG308700 Rh1BG272400 Rh1CG289600 Rh1DG302800
rosa_wichuraiana	Rw1G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	233
Acc16I	TGCGCA	1 cut(s)	1478
AccB1I	GGYRCC	4 cut(s)	65, 128, 668, 1073
AccB7I	CCANNNNNTGG	1 cut(s)	1391
AccBSI	CCGCTC	3 cut(s)	179, 194, 223
AccII	CGCG	5 cut(s)	56, 105, 206, 315, 343
AclWI	GGATC	3 cut(s)	152, 1223, 1236
AcoI	YGGCCR	2 cut(s)	3, 1214
AcsI	RAATTY	2 cut(s)	1764, 1829
AcuI	CTGAAG	2 cut(s)	437, 1397
AcvI	CACGTG	1 cut(s)	1754
AcyI	GRCGYC	4 cut(s)	66, 129, 264, 276
AfaI	GTAC	9 cut(s)	377, 530, 554, 960, 969, 1176, 1558, 1670, 1808
AfeI	AGCGCT	1 cut(s)	211
AfiI	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
AflIII	ACRYGT	3 cut(s)	583, 1030, 1753
AgsI	TTSAA	6 cut(s)	547, 664, 787, 1763, 1846, 1855
AjiI	CACGTC	2 cut(s)	707, 1031
AjnI	CCWGG	2 cut(s)	638, 1288
AleI	CACNNNNGTG	1 cut(s)	674
AluBI	AGCT	8 cut(s)	35, 355, 415, 622, 660, 743, 1411, 1463
AluI	AGCT	8 cut(s)	35, 355, 415, 622, 660, 743, 1411, 1463
Alw21I	GWGCWC	2 cut(s)	731, 1660
Alw26I	GTCTC	2 cut(s)	822, 1422
Alw44I	GTGCAC	1 cut(s)	727
AlwI	GGATC	3 cut(s)	152, 1223, 1236
Aor51HI	AGCGCT	1 cut(s)	211
AoxI	GGCC	6 cut(s)	3, 189, 289, 301, 887, 1214
ApaLI	GTGCAC	1 cut(s)	727
ApeKI	GCWGC	9 cut(s)	35, 734, 749, 881, 951, 1358, 1526, 1529, 1577
ApoI	RAATTY	2 cut(s)	1764, 1829
AspLEI	GCGC	8 cut(s)	68, 131, 206, 212, 251, 315, 436, 1479
AspS9I	GGNCC	4 cut(s)	113, 345, 887, 1438
AsuC2I	CCSGG	1 cut(s)	886
AsuHPI	GGTGA	1 cut(s)	274
AsuNHI	GCTAGC	1 cut(s)	797
AvaII	GGWCC	3 cut(s)	113, 345, 1438
AxyI	CCTNAGG	1 cut(s)	990
BaeGI	GKGCMC	2 cut(s)	671, 731
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	1228
BanI	GGYRCC	4 cut(s)	65, 128, 668, 1073
BbrPI	CACGTG	1 cut(s)	1754
Bbv12I	GWGCWC	2 cut(s)	731, 1660
BbvCI	CCTCAGC	1 cut(s)	1641
BbvI	GCAGC	9 cut(s)	22, 721, 761, 868, 963, 1370, 1513, 1541, 1564
BccI	CCATC	7 cut(s)	532, 680, 907, 1019, 1240, 1428, 1510
BceAI	ACGGC	4 cut(s)	116, 263, 374, 722
BcgI	CGANNNNNNTGC	4 cut(s)	286, 320, 1712, 1746
BciT130I	CCWGG	2 cut(s)	640, 1290
BciVI	GTATCC	1 cut(s)	1616
BcnI	CCSGG	1 cut(s)	886
BcoDI	GTCTC	2 cut(s)	822, 1422
BfaI	CTAG	5 cut(s)	174, 798, 1634, 1698, 1797
BfmI	CTRYAG	2 cut(s)	1509, 1527
BfoI	RGCGCY	4 cut(s)	69, 132, 213, 252
BfuI	GTATCC	1 cut(s)	1616
BglI	GCCNNNNNGGC	1 cut(s)	56
BglII	AGATCT	1 cut(s)	1703
BmcAI	AGTACT	1 cut(s)	1670
Bme1390I	CCNGG	3 cut(s)	640, 886, 1290
Bme18I	GGWCC	3 cut(s)	113, 345, 1438
BmgBI	CACGTC	2 cut(s)	707, 1031
BmgT120I	GGNCC	4 cut(s)	113, 345, 887, 1438
BmiI	GGNNCC	8 cut(s)	67, 115, 130, 670, 1075, 1230, 1439, 1440
BmrFI	CCNGG	3 cut(s)	640, 886, 1290
BmsI	GCATC	8 cut(s)	306, 916, 1135, 1263, 1336, 1454, 1546, 1720
BmtI	GCTAGC	1 cut(s)	801
BoxI	GACNNNNGTC	1 cut(s)	705
BplI	GAGNNNNNCTC	4 cut(s)	308, 340, 1241, 1273
BpmI	CTGGAG	2 cut(s)	62, 1243
Bpu10I	CCTNAGC	2 cut(s)	183, 1641
BpuEI	CTTGAG	1 cut(s)	458
BpuMI	CCSGG	1 cut(s)	886
BsaAI	YACGTR	1 cut(s)	1754
BsaHI	GRCGYC	4 cut(s)	66, 129, 264, 276
BsaJI	CCNNGG	9 cut(s)	54, 103, 124, 672, 1049, 1330, 1492, 1618, 1824
BsaWI	WCCGGW	1 cut(s)	254
Bsc4I	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
Bse1I	ACTGG	3 cut(s)	518, 539, 1146
Bse21I	CCTNAGG	1 cut(s)	990
Bse3DI	GCAATG	4 cut(s)	115, 1581, 1692, 1745
BseBI	CCWGG	2 cut(s)	640, 1290
BseDI	CCNNGG	9 cut(s)	54, 103, 124, 672, 1049, 1330, 1492, 1618, 1824
BseGI	GGATG	7 cut(s)	543, 796, 1030, 1129, 1150, 1400, 1439
BseLI	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
BseMI	GCAATG	4 cut(s)	115, 1581, 1692, 1745
BseMII	CTCAG	5 cut(s)	197, 981, 1239, 1601, 1632
BseNI	ACTGG	3 cut(s)	518, 539, 1146
BseRI	GAGGAG	3 cut(s)	65, 109, 455
BseSI	GKGCMC	2 cut(s)	671, 731
BseXI	GCAGC	9 cut(s)	22, 721, 761, 868, 963, 1370, 1513, 1541, 1564
BsgI	GTGCAG	1 cut(s)	768
Bsh1236I	CGCG	5 cut(s)	56, 105, 206, 315, 343
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	6 cut(s)	5, 191, 291, 303, 889, 1216
BshNI	GGYRCC	4 cut(s)	65, 128, 668, 1073
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	2 cut(s)	731, 1660
BsiSI	CCGG	2 cut(s)	255, 885
BslFI	GGGAC	4 cut(s)	1071, 1451, 1528, 1802
BslI	CCNNNNNNNGG	5 cut(s)	639, 953, 1055, 1336, 1391
BsmAI	GTCTC	2 cut(s)	822, 1422
BsmBI	CGTCTC	1 cut(s)	1422
BsmFI	GGGAC	4 cut(s)	1071, 1451, 1528, 1802
BsmI	GAATGC	2 cut(s)	469, 883
BsnI	GGCC	6 cut(s)	5, 191, 291, 303, 889, 1216
Bsp1286I	GDGCHC	3 cut(s)	671, 731, 1660
Bsp1407I	TGTACA	1 cut(s)	958
Bsp143I	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
Bsp19I	CCATGG	3 cut(s)	1049, 1492, 1618
BspANI	GGCC	6 cut(s)	5, 191, 291, 303, 889, 1216
BspCNI	CTCAG	5 cut(s)	196, 982, 1240, 1600, 1633
BspFNI	CGCG	5 cut(s)	56, 105, 206, 315, 343
BspLI	GGNNCC	8 cut(s)	67, 115, 130, 670, 1075, 1230, 1439, 1440
BspMAI	CTGCAG	2 cut(s)	1513, 1531
BspOI	GCTAGC	1 cut(s)	801
BspPI	GGATC	3 cut(s)	152, 1223, 1236
BspT107I	GGYRCC	4 cut(s)	65, 128, 668, 1073
BsrBI	CCGCTC	3 cut(s)	179, 194, 223
BsrDI	GCAATG	4 cut(s)	115, 1581, 1692, 1745
BsrGI	TGTACA	1 cut(s)	958
BsrI	ACTGG	3 cut(s)	518, 539, 1146
BssECI	CCNNGG	9 cut(s)	54, 103, 124, 672, 1049, 1330, 1492, 1618, 1824
BssMI	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
BssNI	GRCGYC	4 cut(s)	66, 129, 264, 276
BssT1I	CCWWGG	4 cut(s)	1049, 1492, 1618, 1824
Bst2UI	CCWGG	2 cut(s)	640, 1290
Bst4CI	ACNGT	6 cut(s)	504, 673, 1331, 1456, 1655, 1673
Bst6I	CTCTTC	3 cut(s)	331, 1107, 1601
BstACI	GRCGYC	4 cut(s)	66, 129, 264, 276
BstAPI	GCANNNNNTGC	1 cut(s)	1346
BstAUI	TGTACA	1 cut(s)	958
BstBAI	YACGTR	1 cut(s)	1754
BstDEI	CTNAG	7 cut(s)	183, 990, 1020, 1248, 1587, 1641, 1864
BstDSI	CCRYGG	7 cut(s)	54, 103, 672, 1049, 1330, 1492, 1618
BstF5I	GGATG	7 cut(s)	543, 796, 1030, 1129, 1150, 1400, 1439
BstFNI	CGCG	5 cut(s)	56, 105, 206, 315, 343
BstH2I	RGCGCY	4 cut(s)	69, 132, 213, 252
BstHHI	GCGC	8 cut(s)	68, 131, 206, 212, 251, 315, 436, 1479
BstKTI	GATC	6 cut(s)	147, 922, 1231, 1267, 1419, 1706
BstMAI	GTCTC	2 cut(s)	822, 1422
BstMBI	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
BstMCI	CGRYCG	1 cut(s)	103
BstNI	CCWGG	2 cut(s)	640, 1290
BstNSI	RCATGY	1 cut(s)	587
BstPAI	GACNNNNGTC	1 cut(s)	705
BstSCI	CCNGG	3 cut(s)	638, 884, 1288
BstSFI	CTRYAG	2 cut(s)	1509, 1527
BstSLI	GKGCMC	2 cut(s)	671, 731
BstUI	CGCG	5 cut(s)	56, 105, 206, 315, 343
BstV1I	GCAGC	9 cut(s)	22, 721, 761, 868, 963, 1370, 1513, 1541, 1564
BstX2I	RGATCY	2 cut(s)	1228, 1703
BstXI	CCANNNNNNTGG	1 cut(s)	1553
BstYI	RGATCY	2 cut(s)	1228, 1703
Bsu36I	CCTNAGG	1 cut(s)	990
BsuI	GTATCC	1 cut(s)	1616
BsuRI	GGCC	6 cut(s)	5, 191, 291, 303, 889, 1216
BtgI	CCRYGG	7 cut(s)	54, 103, 672, 1049, 1330, 1492, 1618
BtrI	CACGTC	2 cut(s)	707, 1031
BtsCI	GGATG	7 cut(s)	543, 796, 1030, 1129, 1150, 1400, 1439
BtsI	GCAGTG	4 cut(s)	729, 759, 1518, 1521
BtsIMutI	CAGTG	6 cut(s)	729, 759, 1245, 1518, 1521, 1725
CfoI	GCGC	8 cut(s)	68, 131, 206, 212, 251, 315, 436, 1479
Cfr13I	GGNCC	4 cut(s)	113, 345, 887, 1438
Cfr42I	CCGCGG	2 cut(s)	57, 106
CseI	GACGC	5 cut(s)	11, 34, 253, 284, 710
Csp6I	GTAC	9 cut(s)	376, 529, 553, 959, 968, 1175, 1557, 1669, 1807
CviQI	GTAC	9 cut(s)	376, 529, 553, 959, 968, 1175, 1557, 1669, 1807
DdeI	CTNAG	7 cut(s)	183, 990, 1020, 1248, 1587, 1641, 1864
DinI	GGCGCC	2 cut(s)	67, 130
DpnI	GATC	6 cut(s)	146, 921, 1230, 1266, 1418, 1705
DpnII	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
DrdI	GACNNNNNNGTC	1 cut(s)	233
DseDI	GACNNNNNNGTC	1 cut(s)	233
EaeI	YGGCCR	2 cut(s)	3, 1214
Eam1104I	CTCTTC	3 cut(s)	331, 1107, 1601
EarI	CTCTTC	3 cut(s)	331, 1107, 1601
EciI	GGCGGA	1 cut(s)	82
Eco130I	CCWWGG	4 cut(s)	1049, 1492, 1618, 1824
Eco147I	AGGCCT	1 cut(s)	291
Eco47I	GGWCC	3 cut(s)	113, 345, 1438
Eco47III	AGCGCT	1 cut(s)	211
Eco57I	CTGAAG	2 cut(s)	437, 1397
Eco72I	CACGTG	1 cut(s)	1754
Eco81I	CCTNAGG	1 cut(s)	990
EcoO109I	RGGNCCY	1 cut(s)	1438
EcoRII	CCWGG	2 cut(s)	638, 1288
EcoT14I	CCWWGG	4 cut(s)	1049, 1492, 1618, 1824
EcoT22I	ATGCAT	2 cut(s)	497, 1552
EgeI	GGCGCC	2 cut(s)	67, 130
EheI	GGCGCC	2 cut(s)	67, 130
ErhI	CCWWGG	4 cut(s)	1049, 1492, 1618, 1824
Esp3I	CGTCTC	1 cut(s)	1422
FaqI	GGGAC	4 cut(s)	1071, 1451, 1528, 1802
FauI	CCCGC	1 cut(s)	228
FauNDI	CATATG	1 cut(s)	1552
FokI	GGATG	7 cut(s)	550, 803, 1037, 1136, 1157, 1407, 1446
FspAI	RTGCGCAY	1 cut(s)	1478
FspBI	CTAG	5 cut(s)	174, 798, 1634, 1698, 1797
FspI	TGCGCA	1 cut(s)	1478
GlaI	GCGC	8 cut(s)	67, 130, 205, 211, 250, 314, 435, 1478
GsuI	CTGGAG	2 cut(s)	62, 1243
HaeII	RGCGCY	4 cut(s)	69, 132, 213, 252
HaeIII	GGCC	6 cut(s)	5, 191, 291, 303, 889, 1216
HapII	CCGG	2 cut(s)	255, 885
HgaI	GACGC	5 cut(s)	11, 34, 253, 284, 710
HhaI	GCGC	8 cut(s)	68, 131, 206, 212, 251, 315, 436, 1479
Hin1I	GRCGYC	4 cut(s)	66, 129, 264, 276
Hin6I	GCGC	8 cut(s)	66, 129, 204, 210, 249, 313, 434, 1477
HinP1I	GCGC	8 cut(s)	66, 129, 204, 210, 249, 313, 434, 1477
HindIII	AAGCTT	2 cut(s)	658, 1409
HinfI	GANTC	7 cut(s)	576, 611, 822, 899, 1082, 1129, 1676
HpaII	CCGG	2 cut(s)	255, 885
HphI	GGTGA	1 cut(s)	274
Hpy166II	GTNNAC	6 cut(s)	348, 449, 529, 729, 959, 1320
Hpy188I	TCNGA	7 cut(s)	32, 198, 807, 827, 1380, 1416, 1590
Hpy8I	GTNNAC	6 cut(s)	348, 449, 529, 729, 959, 1320
Hpy99I	CGWCG	8 cut(s)	27, 50, 269, 311, 340, 343, 875, 1424
HpyAV	CCTTC	5 cut(s)	93, 97, 637, 658, 781
HpyCH4III	ACNGT	6 cut(s)	504, 673, 1331, 1456, 1655, 1673
HpyCH4IV	ACGT	5 cut(s)	335, 706, 1030, 1188, 1753
HpyF3I	CTNAG	7 cut(s)	183, 990, 1020, 1248, 1587, 1641, 1864
HpySE526I	ACGT	5 cut(s)	335, 706, 1030, 1188, 1753
Hsp92I	GRCGYC	4 cut(s)	66, 129, 264, 276
HspAI	GCGC	8 cut(s)	66, 129, 204, 210, 249, 313, 434, 1477
KasI	GGCGCC	2 cut(s)	65, 128
KflI	GGGWCCC	1 cut(s)	1438
KspI	CCGCGG	2 cut(s)	57, 106
Kzo9I	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
LmnI	GCTCC	2 cut(s)	32, 442
Lsp1109I	GCAGC	9 cut(s)	22, 721, 761, 868, 963, 1370, 1513, 1541, 1564
LweI	GCATC	8 cut(s)	306, 916, 1135, 1263, 1336, 1454, 1546, 1720
MaeI	CTAG	5 cut(s)	174, 798, 1634, 1698, 1797
MaeII	ACGT	5 cut(s)	335, 706, 1030, 1188, 1753
MaeIII	GTNAC	4 cut(s)	280, 647, 896, 1297
MalI	GATC	6 cut(s)	146, 921, 1230, 1266, 1418, 1705
MbiI	CCGCTC	3 cut(s)	179, 194, 223
MboI	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
MboII	GAAGA	8 cut(s)	318, 922, 1094, 1097, 1292, 1355, 1495, 1618
MfeI	CAATTG	2 cut(s)	810, 1065
MflI	RGATCY	2 cut(s)	1228, 1703
MhlI	GDGCHC	3 cut(s)	671, 731, 1660
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	357, 810, 1065, 1764, 1792, 1829
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	66, 129
MlyI	GAGTC	2 cut(s)	570, 893
MmeI	TCCRAC	3 cut(s)	240, 697, 1110
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	497, 1552
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	6 cut(s)	396, 711, 759, 905, 1164, 1353
MslI	CAYNNNNRTG	4 cut(s)	366, 674, 1122, 1551
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	4 cut(s)	56, 105, 415, 954
MspI	CCGG	2 cut(s)	255, 885
MspR9I	CCNGG	3 cut(s)	640, 886, 1290
MunI	CAATTG	2 cut(s)	810, 1065
Mva1269I	GAATGC	2 cut(s)	469, 883
MvaI	CCWGG	2 cut(s)	640, 1290
MvnI	CGCG	5 cut(s)	56, 105, 206, 315, 343
NarI	GGCGCC	2 cut(s)	66, 129
NciI	CCSGG	1 cut(s)	886
NcoI	CCATGG	3 cut(s)	1049, 1492, 1618
NdeI	CATATG	1 cut(s)	1552
NdeII	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
NheI	GCTAGC	1 cut(s)	797
NlaIV	GGNNCC	8 cut(s)	67, 115, 130, 670, 1075, 1230, 1439, 1440
NmeAIII	GCCGAG	2 cut(s)	105, 1687
NmuCI	GTSAC	2 cut(s)	280, 896
NsbI	TGCGCA	1 cut(s)	1478
NsiI	ATGCAT	2 cut(s)	497, 1552
NspI	RCATGY	1 cut(s)	587
OliI	CACNNNNGTG	1 cut(s)	674
PceI	AGGCCT	1 cut(s)	291
PciI	ACATGT	1 cut(s)	583
PcsI	WCGNNNNNNNCGW	3 cut(s)	231, 306, 312
PctI	GAATGC	2 cut(s)	469, 883
PfeI	GAWTC	5 cut(s)	611, 822, 1082, 1129, 1676
PflFI	GACNNNGTC	1 cut(s)	278
PflMI	CCANNNNNTGG	1 cut(s)	1391
PleI	GAGTC	2 cut(s)	570, 893
PluTI	GGCGCC	2 cut(s)	69, 132
PmaCI	CACGTG	1 cut(s)	1754
PmlI	CACGTG	1 cut(s)	1754
PpsI	GAGTC	2 cut(s)	570, 893
Ppu21I	YACGTR	1 cut(s)	1754
PpuMI	RGGWCCY	1 cut(s)	1438
PscI	ACATGT	1 cut(s)	583
PshAI	GACNNNNGTC	1 cut(s)	705
Psp5II	RGGWCCY	1 cut(s)	1438
Psp6I	CCWGG	2 cut(s)	638, 1288
PspCI	CACGTG	1 cut(s)	1754
PspGI	CCWGG	2 cut(s)	638, 1288
PspN4I	GGNNCC	8 cut(s)	67, 115, 130, 670, 1075, 1230, 1439, 1440
PspPI	GGNCC	4 cut(s)	113, 345, 887, 1438
PspPPI	RGGWCCY	1 cut(s)	1438
PstI	CTGCAG	2 cut(s)	1513, 1531
PsuI	RGATCY	2 cut(s)	1228, 1703
PsyI	GACNNNGTC	1 cut(s)	278
PvuII	CAGCTG	1 cut(s)	415
RsaI	GTAC	9 cut(s)	377, 530, 554, 960, 969, 1176, 1558, 1670, 1808
RsaNI	GTAC	9 cut(s)	376, 529, 553, 959, 968, 1175, 1557, 1669, 1807
RseI	CAYNNNNRTG	4 cut(s)	366, 674, 1122, 1551
SacII	CCGCGG	2 cut(s)	57, 106
SaqAI	TTAA	6 cut(s)	396, 711, 759, 905, 1164, 1353
Sau3AI	GATC	6 cut(s)	144, 919, 1228, 1264, 1416, 1703
Sau96I	GGNCC	4 cut(s)	113, 345, 887, 1438
ScaI	AGTACT	1 cut(s)	1670
SchI	GAGTC	2 cut(s)	570, 893
ScrFI	CCNGG	3 cut(s)	640, 886, 1290
SduI	GDGCHC	3 cut(s)	671, 731, 1660
SfaNI	GCATC	8 cut(s)	306, 916, 1135, 1263, 1336, 1454, 1546, 1720
SfcI	CTRYAG	2 cut(s)	1509, 1527
SfoI	GGCGCC	2 cut(s)	67, 130
Sfr303I	CCGCGG	2 cut(s)	57, 106
SgrBI	CCGCGG	2 cut(s)	57, 106
SinI	GGWCC	3 cut(s)	113, 345, 1438
SmiMI	CAYNNNNRTG	4 cut(s)	366, 674, 1122, 1551
SmlI	CTYRAG	1 cut(s)	437
SmoI	CTYRAG	1 cut(s)	437
Sse9I	AATT	6 cut(s)	357, 810, 1065, 1764, 1792, 1829
SseBI	AGGCCT	1 cut(s)	291
SspDI	GGCGCC	2 cut(s)	65, 128
SspMI	CTAG	5 cut(s)	174, 798, 1634, 1698, 1797
StuI	AGGCCT	1 cut(s)	291
StyD4I	CCNGG	3 cut(s)	638, 884, 1288
StyI	CCWWGG	4 cut(s)	1049, 1492, 1618, 1824
TaaI	ACNGT	6 cut(s)	504, 673, 1331, 1456, 1655, 1673
TaiI	ACGT	5 cut(s)	338, 709, 1033, 1191, 1756
TaqI	TCGA	4 cut(s)	225, 309, 1660, 1722
TasI	AATT	6 cut(s)	357, 810, 1065, 1764, 1792, 1829
TatI	WGTACW	3 cut(s)	528, 958, 1668
TauI	GCSGC	9 cut(s)	41, 59, 66, 108, 182, 194, 1182, 1314, 1736
TfiI	GAWTC	5 cut(s)	611, 822, 1082, 1129, 1676
Tru1I	TTAA	6 cut(s)	396, 711, 759, 905, 1164, 1353
Tru9I	TTAA	6 cut(s)	396, 711, 759, 905, 1164, 1353
TscAI	CASTG	6 cut(s)	736, 759, 1252, 1518, 1528, 1732
TseFI	GTSAC	2 cut(s)	280, 896
TseI	GCWGC	9 cut(s)	35, 734, 749, 881, 951, 1358, 1526, 1529, 1577
Tsp45I	GTSAC	2 cut(s)	280, 896
TspDTI	ATGAA	6 cut(s)	350, 710, 1030, 1126, 1496, 1681
TspGWI	ACGGA	3 cut(s)	159, 1241, 1446
TspRI	CASTG	6 cut(s)	736, 759, 1252, 1518, 1528, 1732
Tth111I	GACNNNGTC	1 cut(s)	278
Van91I	CCANNNNNTGG	1 cut(s)	1391
VneI	GTGCAC	1 cut(s)	727
VpaK11BI	GGWCC	3 cut(s)	113, 345, 1438
XapI	RAATTY	2 cut(s)	1764, 1829
XbaI	TCTAGA	2 cut(s)	173, 1633
XceI	RCATGY	1 cut(s)	587
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1831
XspI	CTAG	5 cut(s)	174, 798, 1634, 1698, 1797
ZrmI	AGTACT	1 cut(s)	1670
Zsp2I	ATGCAT	2 cut(s)	497, 1552

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.