MD01G1117300.v1.1

Leukocyte receptor cluster

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009843
Physical Location & Seq
Forward (+)
23105860 .. 23117134
11275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1117300.v1.1.491

Sequence Viewer

Length: 3288 bp
ATGCCCTGCGTTTTGTTTGAAGGTACCGATATTAATTGGCAATCAATTAGGGATGCTAAAGATATGATGAATCAAGGAGGTAATGCTGAGACGATAGCTCCACTGGACCCGAATTCGCTTGAGAATCGGTATAATATTAATGCAAACCAAGGACAGACGCCTTCATATCCTCCTCTTTCAACAGCTGGTTCAGAAGCCTCGTCCTGGAACGTTCATAGGACGGACAATAGCTCGACAGATAATGGGGTTCATTCCCATTCAACCTATCAATACGATCAGCAACCACAGCCGCCTGGGAGTAGTACATCGAGTTTGGGAACAGTAAATGCACCACAAGATTACAATAGCTATGCTTCATACCAACATTCTTCAGATACATATGGTTATGGAAGCGCAGGGTACCCAGGTTACTATAACAACTATCAGCAGCAGTCCAACACTTCCTACTCACAGCCTGTAGGAGCATATCAAAATACAGGTGCTCCTTATCAGCCTCTTTCCTCATTTCAGAATACAGGGTCTTATGCTGGGTCTGCAAGTTATTCAAGCACTTACTACAATCCTGCTGATTATCAGACAGCTGGAGGTTACTCAAGTAGCAGCTACAATAATCAGACTACCGCTTGGAGTGACGGAAATTATGCAAATTATACGCCTCATCAATATTCACAGTACACCCCGGATACCACTGCTGCATATAGTTCTATTACTGCGACTTCAACTTCACAAAATTATGAGCAGCACTACAAGCAATGGGCAGATTATTACAGTCAAACAGAAGTCAGCTGTGCTCCCGGTACTGAGAACATTTCTGTTACAAGTACACCCAATGCAGGATGTCCGGTTCCAGGTGCTACCACCGGGTATCAAACCTCGGACAGCCAGCTACCCCCACCACCACCACTACCTTCATATGCTCCTTCTTGGAGGCCAGAACCCAGTCCATCTGAATTGCCATCAGTGCAGTCTGGTGCTGTAGTTATTGGTGCTCATGATGGTTACTGGAATCATGGGGCTCCAACTTCTCAATCTCAAGTTCACCACAATAGTTCCATGCAACAACACATCCAAAAGCCTATGGATGAAAAGGCTTCTTATGATAGCTTTCAGGATCACCAGAAAACTGCATTTTCTCAAGCACCCAACATGCAGTATCCTGCTTCTCAGCCGGTTCCCCATGCGTCTCATACTTACCAATCACCCTTGCAACCTGTTCGATCCCATGCGTCTCATACTTACCAATCACCCTCACAACCTGTTCCATCCCATGCGTCTCATACTTATCAATCACTGTCCCAACCTGTTCCATCCCATGTGTCTCATACTTATCAGTCACCCTTGCAACCTGTTCCATCCCATGCGTCTCATACTTACCAATCACCCTCCAAACCTGCTCCACCTATAGGTACACAAAGAGATAATAAACTGCAGATTCCAACAAACCCTAGAATCACTTCAAATCCAACCTTTGGGTTACCAAAAACTGACAAGGATAGCTGTACAACCACTGCAGCAGCAAAACCTGCTTATATCAGTGTTTCACTGCCAAAGCCGGTTGACAAGGTGACATCCAGTGCCACTGCTGATTCTCTACTTAAGCCTGGTATGTTTCCCAAGTCGCTGTGTGGTTATGTCGAAAGGGCTTTGGCTCGCTATAAAGATGATAGGCAAAAGGCTGCATGTCAGTCTGTCATGACGGAGATTATCACCCAGGCAACTGCTGATGGAACGCTTTACACACGGGACTGGGATACTGAGCCTATTTTTCCTCTGCCTAATGAAGATGCTGTTAATAAAGAGCCTCCCTCCGTGGGCTTTTTACAGTCTTCAAACCTTGTTTCGTCATTGCCAAAGTACAAAAGAAGTCCGAATAAACAATCTAAAAGTAGATGGGAACCTCTACCAGAGGAGAAGCCAGTTGAGAAACCGGCATCTGTCAACAATGACAGTTTAAAATTTTCTTGGATGAATGTCAATGATAAGCAAAGAAAGCCATGGATGGGGAGTGCTGGGTTCAAAGATGGTAATACAAGTAATGGAAAGTTTACTCCACAGGAGCCGAAAAATGGAAGTAAGATAACCCAGAAACCATTTAAGAAGCAGCGCCTTTCTTATGTTTTAAGTACGGTTGAGAACGATGATGCATCTAGTGATAGTGACAAGGAACAAAGTTTAACCGCATATTACACTGGAGCAATGGGCCTTGAGGATTCACCCGAGGAAAGAAAGAGACGTGAAAATCGCTCTAGGCGTTTTGAAAGAGTGCAAGGACATCGGGCACAAAATAATCACTTCAAACCAAAAAAAGCTGGTGGTGGAAACTTGTATGCCAGAAGGGCTACTGCCTTGGTGCTTAGCAAAAATTTTGAAGATGGTGGCAGCAGGGCAGTTGAGGACATCGACTGGGATTCTCTTACTGTTAAGGGCACCTGCCAGGAGATTGAGAAACGTTATTTGCGCCTCACCTCCGCTCCTGATCCTGCCACTGTAAGGCCAGAGGATGTTCTTGAAAAAGCTCTGCTTATGGTACAAAATTCTCAAAAGAATTACCTATATAAATGTGATCAGTTGAAGTCCATTCGTCAGGATTTGACTGTGCAACGAATTCGCAATCACCTAACAGTAAAGGTGTACGAAACCCATGCTCGCTTAGCAGTGGAAGTTGGGGACCTGCCCGAGTATAATCAGTGCGCGTCACAGTTGAAATCTCTCTATGCTGAAGGTATTGAGGGATGCTATATGGAGTTCTCTGCATACAACTTACTTTGTGTTATCCTTCACTCAAATAATAACAGAGATCTAGTATCATCAATGGCAAGCTTGTCAGCTGAAGCGAAAAGAGATGAAGCTGTTAAGCATGCTCTTGCGGTTCGTTCAGCTGTGACTTCGGGAAATTATGTTATGTTCTTCAGGCTCTACAAGACAGCTCCTAACTTGAGCACCTGCCTTATGGATCTCTATGTTGAAAAGATGCGGTATAAGGCAGTGAGCTGCATCTGCAGATCATATCGGCCTACGATACCTGTTCCTTATGTTGCTCAAATTCTGGGATTTACCACCAGTACACCCACAAACGAGGAAAGTGAGGAGAAGGATTCAGAGGGATTGGACGAATGCATTGAATGGTTGAAAGTACACGGTGCATGCCTCACTGTTGATAACAGCGGAGAAATGCAGCTTGATACAAAGCCTACGTCCTCAAGTCTTTACATGCCGGAGACTGATGCGGTGTCCCACGGAGATGCCACTCTCGCAGTTAACGATTTTTTGACAAGGACACCTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

1096

Amino Acids

120.92

Weight (kDa)

6.27

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 818 - 1025 1.7e-23 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 2438, 2951
Acc36I ACCTGC 5 cut(s) 1399, 1531, 2438, 2679, 2951
Acc65I GGTACC 2 cut(s) 23, 399
AccB1I GGYRCC 3 cut(s) 23, 399, 2426
AccB7I CCANNNNNTGG 1 cut(s) 1469
AccBSI CCGCTC 1 cut(s) 2471
AccII CGCG 1 cut(s) 2693
AciI CCGC 8 cut(s) 290, 621, 2178, 2469, 2867, 2974, 3165, 3227
AclI AACGTT 2 cut(s) 210, 2449
AclWI GGATC 4 cut(s) 1119, 1212, 2471, 2961
AcsI RAATTY 6 cut(s) 112, 1955, 2362, 2533, 2604, 3042
AcuI CTGAAG 4 cut(s) 354, 2739, 2850, 2893
AcyI GRCGYC 1 cut(s) 158
AfiI CCNNNNNNNGG 9 cut(s) 204, 833, 848, 1403, 1469, 1811, 2000, 2066, 2490
AflII CTTAAG 1 cut(s) 1595
AjiI CACGTC 1 cut(s) 2234
AjnI CCWGG 7 cut(s) 203, 292, 403, 847, 1600, 1710, 2433
AloI GAACNNNNNNTCC 2 cut(s) 828, 860
Alw21I GWGCWC 4 cut(s) 484, 793, 991, 2942
Alw26I GTCTC 8 cut(s) 83, 1188, 1233, 1278, 1323, 1368, 2224, 3212
AlwI GGATC 4 cut(s) 1119, 1212, 2471, 2961
Ama87I CYCGRG 2 cut(s) 2216, 2675
AoxI GGCC 4 cut(s) 929, 2200, 2492, 3011
ApoI RAATTY 6 cut(s) 112, 1955, 2362, 2533, 2604, 3042
ArsI GACNNNNNNTTYG 2 cut(s) 3179, 3211
AseI ATTAAT 2 cut(s) 33, 138
Asp700I GAANNNNTTC 1 cut(s) 1453
Asp718I GGTACC 2 cut(s) 23, 399
AspLEI GCGC 4 cut(s) 395, 2106, 2460, 2693
AspS9I GGNCC 3 cut(s) 106, 2200, 2668
AsuC2I CCSGG 3 cut(s) 680, 795, 862
AvaI CYCGRG 2 cut(s) 2216, 2675
AvaII GGWCC 2 cut(s) 106, 2668
BaeGI GKGCMC 2 cut(s) 2281, 2429
BaeI ACNNNNGTAYC 2 cut(s) 391, 424
BanI GGYRCC 3 cut(s) 23, 399, 2426
BanII GRGCYC 1 cut(s) 1018
BbsI GAAGAC 1 cut(s) 1818
Bbv12I GWGCWC 4 cut(s) 484, 793, 991, 2942
BcgI CGANNNNNNTGC 8 cut(s) 1196, 1230, 2255, 2289, 2588, 2622, 2624, 2658
BciT130I CCWGG 7 cut(s) 205, 294, 405, 849, 1602, 1712, 2435
BciVI GTATCC 3 cut(s) 676, 1164, 1744
BclI TGATCA 1 cut(s) 2563
BcnI CCSGG 3 cut(s) 680, 795, 862
BcoDI GTCTC 8 cut(s) 83, 1188, 1233, 1278, 1323, 1368, 2224, 3212
BfaI CTAG 4 cut(s) 1446, 2148, 2247, 2801
BfmI CTRYAG 6 cut(s) 456, 975, 1401, 1427, 1509, 2998
BfoI RGCGCY 1 cut(s) 2107
BfrI CTTAAG 1 cut(s) 1595
BfuAI ACCTGC 5 cut(s) 1399, 1531, 2438, 2679, 2951
BfuI GTATCC 3 cut(s) 676, 1164, 1744
BglI GCCNNNNNGGC 1 cut(s) 2336
BglII AGATCT 1 cut(s) 2797
BlpI GCTNAGC 2 cut(s) 2354, 2650
Bme18I GGWCC 2 cut(s) 106, 2668
BmeT110I CYCGRG 2 cut(s) 2216, 2675
BmgBI CACGTC 1 cut(s) 2234
BmgT120I GGNCC 3 cut(s) 106, 2200, 2668
BmrI ACTGGG 3 cut(s) 933, 1756, 2413
BmuI ACTGGG 3 cut(s) 933, 1756, 2413
BpiI GAAGAC 1 cut(s) 1818
BplI GAGNNNNNCTC 2 cut(s) 1790, 1822
BpmI CTGGAG 2 cut(s) 603, 2211
Bpu1102I GCTNAGC 2 cut(s) 2354, 2650
BpuEI CTTGAG 7 cut(s) 140, 577, 1017, 1119, 2225, 2956, 3184
BpuMI CCSGG 3 cut(s) 680, 795, 862
BsaHI GRCGYC 1 cut(s) 158
BsaWI WCCGGW 1 cut(s) 841
BsaXI ACNNNNNCTCC 6 cut(s) 82, 112, 466, 496, 2455, 2485
Bsc4I CCNNNNNNNGG 9 cut(s) 204, 833, 848, 1403, 1469, 1811, 2000, 2066, 2490
Bse118I RCCGGY 3 cut(s) 1168, 1552, 1927
Bse1I ACTGG 9 cut(s) 108, 939, 1007, 1572, 1751, 1916, 2194, 2408, 3060
Bse3DI GCAATG 3 cut(s) 758, 1844, 2202
BseBI CCWGG 7 cut(s) 205, 294, 405, 849, 1602, 1712, 2435
BseLI CCNNNNNNNGG 9 cut(s) 204, 833, 848, 1403, 1469, 1811, 2000, 2066, 2490
BseMI GCAATG 3 cut(s) 758, 1844, 2202
BseMII CTCAG 4 cut(s) 78, 792, 1178, 1746
BseNI ACTGG 9 cut(s) 108, 939, 1007, 1572, 1751, 1916, 2194, 2408, 3060
BseRI GAGGAG 3 cut(s) 162, 1922, 3101
BseSI GKGCMC 2 cut(s) 2281, 2429
BseYI CCCAGC 2 cut(s) 527, 2009
BsgI GTGCAG 1 cut(s) 983
Bsh1236I CGCG 1 cut(s) 2693
BshFI GGCC 4 cut(s) 931, 2202, 2494, 3013
BshNI GGYRCC 3 cut(s) 23, 399, 2426
BsiHKAI GWGCWC 4 cut(s) 484, 793, 991, 2942
BsiHKCI CYCGRG 2 cut(s) 2216, 2675
BsiSI CCGG 8 cut(s) 680, 795, 842, 861, 1169, 1553, 1928, 3215
BslFI GGGAC 4 cut(s) 1279, 1757, 2681, 3217
BslI CCNNNNNNNGG 9 cut(s) 204, 833, 848, 1403, 1469, 1811, 2000, 2066, 2490
BsmAI GTCTC 8 cut(s) 83, 1188, 1233, 1278, 1323, 1368, 2224, 3212
BsmBI CGTCTC 6 cut(s) 83, 1188, 1233, 1278, 1368, 2224
BsmFI GGGAC 4 cut(s) 1279, 1757, 2681, 3217
BsmI GAATGC 1 cut(s) 3119
BsnI GGCC 4 cut(s) 931, 2202, 2494, 3013
BsoBI CYCGRG 2 cut(s) 2216, 2675
Bsp1286I GDGCHC 7 cut(s) 484, 793, 991, 1018, 2281, 2429, 2942
Bsp1407I TGTACA 1 cut(s) 1499
Bsp143I GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
Bsp1720I GCTNAGC 2 cut(s) 2354, 2650
Bsp19I CCATGG 1 cut(s) 1994
BspACI CCGC 8 cut(s) 290, 621, 2178, 2469, 2867, 2974, 3165, 3227
BspANI GGCC 4 cut(s) 931, 2202, 2494, 3013
BspCNI CTCAG 4 cut(s) 79, 793, 1177, 1747
BspFNI CGCG 1 cut(s) 2693
BspHI TCATGA 2 cut(s) 991, 1692
BspMAI CTGCAG 3 cut(s) 1431, 1513, 3002
BspMI ACCTGC 5 cut(s) 1399, 1531, 2438, 2679, 2951
BspPI GGATC 4 cut(s) 1119, 1212, 2471, 2961
BspT107I GGYRCC 3 cut(s) 23, 399, 2426
BspTI CTTAAG 1 cut(s) 1595
BsrBI CCGCTC 1 cut(s) 2471
BsrDI GCAATG 3 cut(s) 758, 1844, 2202
BsrFI RCCGGY 3 cut(s) 1168, 1552, 1927
BsrGI TGTACA 1 cut(s) 1499
BsrI ACTGG 9 cut(s) 108, 939, 1007, 1572, 1751, 1916, 2194, 2408, 3060
BssAI RCCGGY 3 cut(s) 1168, 1552, 1927
BssMI GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
BssNI GRCGYC 1 cut(s) 158
BssT1I CCWWGG 3 cut(s) 148, 1994, 2346
Bst2UI CCWGG 7 cut(s) 205, 294, 405, 849, 1602, 1712, 2435
BstACI GRCGYC 1 cut(s) 158
BstAFI CTTAAG 1 cut(s) 1595
BstAPI GCANNNNNTGC 1 cut(s) 1523
BstAUI TGTACA 1 cut(s) 1499
BstC8I GCNNGC 6 cut(s) 884, 1651, 2647, 2818, 2859, 3145
BstDEI CTNAG 6 cut(s) 87, 801, 1164, 1755, 2354, 2650
BstDSI CCRYGG 3 cut(s) 1809, 1994, 3235
BstEII GGTNACC 1 cut(s) 1473
BstFNI CGCG 1 cut(s) 2693
BstH2I RGCGCY 1 cut(s) 2107
BstHHI GCGC 4 cut(s) 395, 2106, 2460, 2693
BstKTI GATC 8 cut(s) 277, 1114, 1220, 2479, 2566, 2800, 2956, 3005
BstMAI GTCTC 8 cut(s) 83, 1188, 1233, 1278, 1323, 1368, 2224, 3212
BstMBI GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
BstNI CCWGG 7 cut(s) 205, 294, 405, 849, 1602, 1712, 2435
BstNSI RCATGY 5 cut(s) 1150, 1683, 2861, 3147, 3214
BstPI GGTNACC 1 cut(s) 1473
BstSFI CTRYAG 6 cut(s) 456, 975, 1401, 1427, 1509, 2998
BstSLI GKGCMC 2 cut(s) 2281, 2429
BstUI CGCG 1 cut(s) 2693
BstV2I GAAGAC 1 cut(s) 1818
BstX2I RGATCY 2 cut(s) 2797, 2953
BstYI RGATCY 2 cut(s) 2797, 2953
BsuI GTATCC 3 cut(s) 676, 1164, 1744
BsuRI GGCC 4 cut(s) 931, 2202, 2494, 3013
BtgI CCRYGG 3 cut(s) 1809, 1994, 3235
BtrI CACGTC 1 cut(s) 2234
BtsI GCAGTG 6 cut(s) 687, 1506, 1541, 1578, 2661, 2991
BveI ACCTGC 5 cut(s) 1399, 1531, 2438, 2679, 2951
Cac8I GCNNGC 6 cut(s) 884, 1651, 2647, 2818, 2859, 3145
CciI TCATGA 2 cut(s) 991, 1692
CfoI GCGC 4 cut(s) 395, 2106, 2460, 2693
Cfr10I RCCGGY 3 cut(s) 1168, 1552, 1927
Cfr13I GGNCC 3 cut(s) 106, 2200, 2668
CseI GACGC 6 cut(s) 166, 1170, 1215, 1260, 1350, 2682
DdeI CTNAG 6 cut(s) 87, 801, 1164, 1755, 2354, 2650
DpnI GATC 8 cut(s) 276, 1113, 1219, 2478, 2565, 2799, 2955, 3004
DpnII GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
DraI TTTAAA 1 cut(s) 1953
Eco130I CCWWGG 3 cut(s) 148, 1994, 2346
Eco24I GRGCYC 1 cut(s) 1018
Eco47I GGWCC 2 cut(s) 106, 2668
Eco57I CTGAAG 4 cut(s) 354, 2739, 2850, 2893
Eco88I CYCGRG 2 cut(s) 2216, 2675
Eco91I GGTNACC 1 cut(s) 1473
EcoO109I RGGNCCY 1 cut(s) 2668
EcoO65I GGTNACC 1 cut(s) 1473
EcoRI GAATTC 2 cut(s) 112, 2604
EcoRII CCWGG 7 cut(s) 203, 292, 403, 847, 1600, 1710, 2433
EcoT14I CCWWGG 3 cut(s) 148, 1994, 2346
EcoT22I ATGCAT 2 cut(s) 2146, 3119
EcoT38I GRGCYC 1 cut(s) 1018
ErhI CCWWGG 3 cut(s) 148, 1994, 2346
Esp3I CGTCTC 6 cut(s) 83, 1188, 1233, 1278, 1368, 2224
FalI AAGNNNNNCTT 3 cut(s) 2505, 2537, 3265
FaqI GGGAC 4 cut(s) 1279, 1757, 2681, 3217
FauNDI CATATG 2 cut(s) 379, 913
FbaI TGATCA 1 cut(s) 2563
FriOI GRGCYC 1 cut(s) 1018
FspBI CTAG 4 cut(s) 1446, 2148, 2247, 2801
GlaI GCGC 4 cut(s) 394, 2105, 2459, 2692
GsaI CCCAGC 2 cut(s) 531, 2013
GsuI CTGGAG 2 cut(s) 603, 2211
HaeII RGCGCY 1 cut(s) 2107
HaeIII GGCC 4 cut(s) 931, 2202, 2494, 3013
HapII CCGG 8 cut(s) 680, 795, 842, 861, 1169, 1553, 1928, 3215
HgaI GACGC 6 cut(s) 166, 1170, 1215, 1260, 1350, 2682
HhaI GCGC 4 cut(s) 395, 2106, 2460, 2693
Hin1I GRCGYC 1 cut(s) 158
Hin6I GCGC 4 cut(s) 393, 2104, 2458, 2691
HinP1I GCGC 4 cut(s) 393, 2104, 2458, 2691
HincII GTYRAC 3 cut(s) 1558, 1939, 3259
HindII GTYRAC 3 cut(s) 1558, 1939, 3259
HindIII AAGCTT 1 cut(s) 2818
HinfI GANTC 9 cut(s) 70, 124, 1006, 1432, 1449, 1586, 2210, 2408, 3095
HpaI GTTAAC 1 cut(s) 3259
HpaII CCGG 8 cut(s) 680, 795, 842, 861, 1169, 1553, 1928, 3215
Hpy188I TCNGA 9 cut(s) 193, 373, 510, 576, 615, 877, 949, 1869, 3100
Hpy188III TCNNGA 7 cut(s) 992, 1109, 1693, 2474, 2507, 2585, 2889
HpyAV CCTTC 8 cut(s) 14, 171, 918, 930, 2328, 2714, 2786, 3085
HpyCH4IV ACGT 4 cut(s) 210, 2233, 2449, 3194
HpyF3I CTNAG 6 cut(s) 87, 801, 1164, 1755, 2354, 2650
HpySE526I ACGT 4 cut(s) 210, 2233, 2449, 3194
Hsp92I GRCGYC 1 cut(s) 158
HspAI GCGC 4 cut(s) 393, 2104, 2458, 2691
KpnI GGTACC 2 cut(s) 27, 403
Ksp22I TGATCA 1 cut(s) 2563
KspAI GTTAAC 1 cut(s) 3259
Kzo9I GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
MaeI CTAG 4 cut(s) 1446, 2148, 2247, 2801
MaeII ACGT 4 cut(s) 210, 2233, 2449, 3194
MalI GATC 8 cut(s) 276, 1113, 1219, 2478, 2565, 2799, 2955, 3004
MbiI CCGCTC 1 cut(s) 2471
MboI GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
MboII GAAGA 5 cut(s) 360, 1793, 1818, 2381, 2899
MflI RGATCY 2 cut(s) 2797, 2953
MhlI GDGCHC 7 cut(s) 484, 793, 991, 1018, 2281, 2429, 2942
MmeI TCCRAC 4 cut(s) 459, 1043, 1460, 1487
Mph1103I ATGCAT 2 cut(s) 2146, 3119
MroXI GAANNNNTTC 1 cut(s) 1453
MslI CAYNNNNRTG 1 cut(s) 3240
MspA1I CMGCKG 6 cut(s) 185, 581, 786, 2828, 2879, 3165
MspCI CTTAAG 1 cut(s) 1595
MspI CCGG 8 cut(s) 680, 795, 842, 861, 1169, 1553, 1928, 3215
Mva1269I GAATGC 1 cut(s) 3119
MvaI CCWGG 7 cut(s) 205, 294, 405, 849, 1602, 1712, 2435
MvnI CGCG 1 cut(s) 2693
NciI CCSGG 3 cut(s) 680, 795, 862
NcoI CCATGG 1 cut(s) 1994
NdeI CATATG 2 cut(s) 379, 913
NdeII GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
NmuCI GTSAC 6 cut(s) 629, 1332, 1564, 2156, 2694, 2881
NsiI ATGCAT 2 cut(s) 2146, 3119
NspI RCATGY 5 cut(s) 1150, 1683, 2861, 3147, 3214
PaeI GCATGC 2 cut(s) 2861, 3147
PagI TCATGA 2 cut(s) 991, 1692
PaqCI CACCTGC 2 cut(s) 2438, 2951
PcsI WCGNNNNNNNCGW 2 cut(s) 2248, 3258
PctI GAATGC 1 cut(s) 3119
PdmI GAANNNNTTC 1 cut(s) 1453
PfeI GAWTC 9 cut(s) 70, 124, 1006, 1432, 1449, 1586, 2210, 2408, 3095
PflMI CCANNNNNTGG 1 cut(s) 1469
PfoI TCCNGGA 1 cut(s) 203
PpuMI RGGWCCY 1 cut(s) 2668
PshBI ATTAAT 2 cut(s) 33, 138
Psp1406I AACGTT 2 cut(s) 210, 2449
Psp5II RGGWCCY 1 cut(s) 2668
Psp6I CCWGG 7 cut(s) 203, 292, 403, 847, 1600, 1710, 2433
PspEI GGTNACC 1 cut(s) 1473
PspFI CCCAGC 2 cut(s) 527, 2009
PspGI CCWGG 7 cut(s) 203, 292, 403, 847, 1600, 1710, 2433
PspPI GGNCC 3 cut(s) 106, 2200, 2668
PspPPI RGGWCCY 1 cut(s) 2668
PstI CTGCAG 3 cut(s) 1431, 1513, 3002
PsuI RGATCY 2 cut(s) 2797, 2953
PvuII CAGCTG 5 cut(s) 185, 581, 786, 2828, 2879
RseI CAYNNNNRTG 1 cut(s) 3240
Sau3AI GATC 8 cut(s) 274, 1111, 1217, 2476, 2563, 2797, 2953, 3002
Sau96I GGNCC 3 cut(s) 106, 2200, 2668
SduI GDGCHC 7 cut(s) 484, 793, 991, 1018, 2281, 2429, 2942
SfcI CTRYAG 6 cut(s) 456, 975, 1401, 1427, 1509, 2998
SinI GGWCC 2 cut(s) 106, 2668
SmiMI CAYNNNNRTG 1 cut(s) 3240
SmlI CTYRAG 8 cut(s) 119, 592, 1032, 1134, 1595, 2204, 2935, 3199
SmoI CTYRAG 8 cut(s) 119, 592, 1032, 1134, 1595, 2204, 2935, 3199
SphI GCATGC 2 cut(s) 2861, 3147
SsiI CCGC 8 cut(s) 290, 621, 2178, 2469, 2867, 2974, 3165, 3227
SspI AATATT 2 cut(s) 136, 665
SspMI CTAG 4 cut(s) 1446, 2148, 2247, 2801
StyI CCWWGG 3 cut(s) 148, 1994, 2346
TaiI ACGT 4 cut(s) 213, 2236, 2452, 3197
TaqI TCGA 5 cut(s) 233, 308, 1216, 1635, 2400
TatI WGTACW 7 cut(s) 302, 672, 821, 1499, 1854, 3062, 3133
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 9 cut(s) 70, 124, 1006, 1432, 1449, 1586, 2210, 2408, 3095
TseFI GTSAC 6 cut(s) 629, 1332, 1564, 2156, 2694, 2881
Tsp45I GTSAC 6 cut(s) 629, 1332, 1564, 2156, 2694, 2881
TspGWI ACGGA 5 cut(s) 236, 648, 1712, 1798, 3252
Van91I CCANNNNNTGG 1 cut(s) 1469
Vha464I CTTAAG 1 cut(s) 1595
VpaK11BI GGWCC 2 cut(s) 106, 2668
VspI ATTAAT 2 cut(s) 33, 138
XapI RAATTY 6 cut(s) 112, 1955, 2362, 2533, 2604, 3042
XceI RCATGY 5 cut(s) 1150, 1683, 2861, 3147, 3214
XmnI GAANNNNTTC 1 cut(s) 1453
XspI CTAG 4 cut(s) 1446, 2148, 2247, 2801
Zsp2I ATGCAT 2 cut(s) 2146, 3119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.