MD01G1207300.v1.1

Protein of unknown function (DUF819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0017890
Physical Location & Seq
Reverse (-)
30256173 .. 30259126
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1207300.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGGCTTCTCTCCCCGTCACATGGCCGTTACTCCACCTTCCCGCCACTCCCACTCCATCACTCTCACCAGTCAAACGAGTCAAACCCAACCCTTTTCCGTCTCACGCCGTTAATGGAACGCTTCTCTTGCCGTTGACTCGTCCTCGGAGCCGCAAATGCGTCCAAGCGACAAGGGTAATTACGTCACAGCTCAGGCAGCCTATCGTCGCTCCGGATGACCACTGGGGCTTATGGACCGCCCTGTTCGCCACCGGTGCTTTTGGTCTCTGGTCCGAGAAGACGAAGATTGGTGGGATGGTGAGTGCTGCCCTTGTAAGCACTTTGGTTGGGCTTGCAGCAAGTAATTTTGGCATAATTCCTTTTGAAGCGCCTGCATATTCCATTGTCTTGGAGTTCCTTCTACCATTATCTATTCCATTGCTATTATATAGAGCTGACTTGCGCAGTGTGATGCGGTCCACGGGGACTTTGCTCTTAGCTTTCGTGCTTGGATCAGTTGCGACTATGGTTGGAACTCTCGTGGCATTTCAAATGGTGCCCATGCGATCACTTGGTCCAGATAATTGGAAAATAGCTTCTGCTCTCATGGGTAGCTATATTGGTGGATCTGTTAATTATGTTGCGATTTCAGAGGCTCTCGGTGTTTCTCCATCGGTCATGGCTGCAGGAGTAGCAGCAGACAATGTTATTTGTGCTCTGTATTTCATGGTGTTGTTTGCCTTGGCCTCTAAAATACCTCCCGAGGCCTCAACATCAACTGATGATGTTTCAATGCAAACGGAATCCAATTACGACAGAAAGCTGCCTGTTGTAGAAACTGCCACTGCTCTTGCTACATCGTTTGTGATATGCAAGGCTTCCACATATCTCACAGGATTGTGTGGAATTCCAGGAGGCAGCTTGCCAATGATAACAGCCGCTGTTGTCATATTAGCAACTCTTCTCCCGACACGTTTCGCTTACCTTGCACCTGCTGGGGATACCGTTGCTTTGCTTTTGATGCAGGTATTCTTCGCAGTGTTGGGGGCTAGCGGGAGCATATGGAATGTCATCAACACCGCACCTAGTATCTTTTTCTTTGCTCTAGTTCAAGTGACCGTCCATCTTGCCGTGGTACTTGGATTGGGAAAGCTATTTCACTTGGATTTAAAACTGTTACTCTTGGCTTCCAACGCCAATATCGGAGGGCCAACGACAGCAGGCGGCATGGCCGGGACCAAGGGATGGGATTCTTTGGTTGTTCCTGCAATCCTTGCAGGCATATTTGGAATTGCCATTGCAACATTTCTTGGCATTGGTTTTGGAATGATAGTCCTCAGACGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF819

Protein Analysis

443

Amino Acids

46.29

Weight (kDa)

9.41

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF819 PF05684 79 - 440 2.9e-119 Protein of unknown function (DUF819)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24000
fragaria_vesca FvH4_7g30150
malus_domestica MD01G1207300.v1.1
pyrus_communis pycom01g21820
rosa_chinensis RchiOBHm_Chr1g0378181
rosa_laevigata RLG00000026464
rosa_multiflora Rmu_sc0000625.1_g000011
rosa_roxburghii Rroxscaffold_4G00280690
rosa_rugosa Rorug01G0406800
rosa_samantha Rh1BG385900
rosa_wichuraiana Rw1G037440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 979
Acc16I TGCGCA 1 cut(s) 443
Acc36I ACCTGC 2 cut(s) 979, 994
AccB1I GGYRCC 1 cut(s) 535
AccB7I CCANNNNNTGG 1 cut(s) 1224
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 8 cut(s) 42, 151, 237, 454, 918, 1032, 1059, 1203
AclWI GGATC 2 cut(s) 499, 613
AcoI YGGCCR 2 cut(s) 23, 1209
AcsI RAATTY 1 cut(s) 885
AfaI GTAC 1 cut(s) 1116
AfiI CCNNNNNNNGG 2 cut(s) 21, 1224
AflIII ACRYGT 1 cut(s) 950
AgeI ACCGGT 1 cut(s) 251
AgsI TTSAA 4 cut(s) 365, 530, 771, 1091
AjnI CCWGG 1 cut(s) 889
AluBI AGCT 8 cut(s) 190, 434, 479, 575, 594, 802, 900, 1132
AluI AGCT 8 cut(s) 190, 434, 479, 575, 594, 802, 900, 1132
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 2 cut(s) 105, 269
AlwI GGATC 2 cut(s) 499, 613
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 1 cut(s) 740
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 5 cut(s) 23, 723, 744, 1187, 1209
ApeKI GCWGC 7 cut(s) 196, 305, 335, 662, 674, 802, 897
ApoI RAATTY 1 cut(s) 885
AsiGI ACCGGT 1 cut(s) 251
AspLEI GCGC 2 cut(s) 370, 444
AspS9I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
AsuC2I CCSGG 1 cut(s) 1213
AsuHPI GGTGA 2 cut(s) 57, 310
AsuNHI GCTAGC 1 cut(s) 1028
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 5 cut(s) 234, 270, 456, 554, 1215
BaeGI GKGCMC 1 cut(s) 540
BanI GGYRCC 1 cut(s) 535
BauI CACGAG 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 7 cut(s) 208, 292, 347, 649, 686, 789, 909
BccI CCATC 5 cut(s) 64, 289, 658, 1110, 1218
BceAI ACGGC 4 cut(s) 10, 92, 115, 1094
BciT130I CCWGG 1 cut(s) 891
BciVI GTATCC 1 cut(s) 973
BcnI CCSGG 1 cut(s) 1213
BcoDI GTCTC 2 cut(s) 105, 269
BfaI CTAG 3 cut(s) 1029, 1065, 1085
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 371
BfuAI ACCTGC 2 cut(s) 979, 994
BfuI GTATCC 1 cut(s) 973
Bme1390I CCNGG 2 cut(s) 891, 1213
Bme18I GGWCC 5 cut(s) 234, 270, 456, 554, 1215
BmeT110I CYCGRG 1 cut(s) 740
BmgT120I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
BmiI GGNNCC 3 cut(s) 149, 537, 1216
BmrFI CCNGG 2 cut(s) 891, 1213
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 2 cut(s) 441, 990
BmtI GCTAGC 1 cut(s) 1032
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 1213
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 6 cut(s) 143, 459, 720, 741, 1110, 1218
BsaWI WCCGGW 2 cut(s) 211, 251
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 1224
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 68, 227
Bse3DI GCAATG 2 cut(s) 416, 1275
BseAI TCCGGA 1 cut(s) 211
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 6 cut(s) 143, 459, 720, 741, 1110, 1218
BseGI GGATG 3 cut(s) 220, 300, 1229
BseLI CCNNNNNNNGG 2 cut(s) 21, 1224
BseMI GCAATG 2 cut(s) 416, 1275
BseMII CTCAG 1 cut(s) 205
BseNI ACTGG 2 cut(s) 68, 227
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 7 cut(s) 208, 292, 347, 649, 686, 789, 909
BseYI CCCAGC 1 cut(s) 974
BshFI GGCC 5 cut(s) 25, 725, 746, 1189, 1211
BshNI GGYRCC 1 cut(s) 535
BshTI ACCGGT 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 3 cut(s) 212, 252, 1212
BslFI GGGAC 2 cut(s) 478, 1228
BslI CCNNNNNNNGG 2 cut(s) 21, 1224
BsmAI GTCTC 2 cut(s) 105, 269
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 2 cut(s) 478, 1228
BsnI GGCC 5 cut(s) 25, 725, 746, 1189, 1211
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 740
Bsp1286I GDGCHC 2 cut(s) 540, 697
Bsp13I TCCGGA 1 cut(s) 211
Bsp143I GATC 3 cut(s) 491, 545, 605
BspACI CCGC 8 cut(s) 42, 151, 237, 454, 918, 1032, 1059, 1203
BspANI GGCC 5 cut(s) 25, 725, 746, 1189, 1211
BspCNI CTCAG 2 cut(s) 204, 1329
BspEI TCCGGA 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 149, 537, 1216
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 2 cut(s) 979, 994
BspOI GCTAGC 1 cut(s) 1032
BspPI GGATC 2 cut(s) 499, 613
BspT107I GGYRCC 1 cut(s) 535
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 2 cut(s) 416, 1275
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 2 cut(s) 68, 227
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 6 cut(s) 143, 459, 720, 741, 1110, 1218
BssMI GATC 3 cut(s) 491, 545, 605
BssSI CACGAG 1 cut(s) 518
BssT1I CCWWGG 2 cut(s) 720, 1218
Bst2BI CACGAG 1 cut(s) 518
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 3 cut(s) 985, 1099, 1155
Bst6I CTCTTC 1 cut(s) 945
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstC8I GCNNGC 6 cut(s) 333, 372, 902, 1030, 1201, 1258
BstDEI CTNAG 3 cut(s) 191, 475, 1316
BstDSI CCRYGG 2 cut(s) 459, 1110
BstF5I GGATG 3 cut(s) 220, 300, 1229
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 2 cut(s) 370, 444
BstKTI GATC 3 cut(s) 494, 548, 608
BstMAI GTCTC 2 cut(s) 105, 269
BstMBI GATC 3 cut(s) 491, 545, 605
BstNI CCWGG 1 cut(s) 891
BstSCI CCNGG 2 cut(s) 889, 1211
BstSFI CTRYAG 1 cut(s) 663
BstSLI GKGCMC 1 cut(s) 540
BstV1I GCAGC 7 cut(s) 208, 292, 347, 649, 686, 789, 909
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 605
BstXI CCANNNNNNTGG 2 cut(s) 388, 564
BstYI RGATCY 1 cut(s) 605
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 5 cut(s) 25, 725, 746, 1189, 1211
BtgI CCRYGG 2 cut(s) 459, 1110
BtsCI GGATG 3 cut(s) 220, 300, 1229
BtsI GCAGTG 3 cut(s) 451, 822, 1023
BtsIMutI CAGTG 4 cut(s) 220, 451, 822, 1023
BveI ACCTGC 2 cut(s) 979, 994
Cac8I GCNNGC 6 cut(s) 333, 372, 902, 1030, 1201, 1258
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 2 cut(s) 370, 444
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
CseI GACGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 1115
CspAI ACCGGT 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 811, 846
CviAII CATG 6 cut(s) 21, 541, 586, 658, 706, 1207
CviQI GTAC 1 cut(s) 1115
DdeI CTNAG 3 cut(s) 191, 475, 1316
DpnI GATC 3 cut(s) 493, 547, 607
DpnII GATC 3 cut(s) 491, 545, 605
DraI TTTAAA 1 cut(s) 1149
EaeI YGGCCR 2 cut(s) 23, 1209
Eam1104I CTCTTC 1 cut(s) 945
EarI CTCTTC 1 cut(s) 945
Eco130I CCWWGG 2 cut(s) 720, 1218
Eco147I AGGCCT 1 cut(s) 746
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 5 cut(s) 234, 270, 456, 554, 1215
Eco88I CYCGRG 1 cut(s) 740
EcoRI GAATTC 1 cut(s) 885
EcoRII CCWGG 1 cut(s) 889
EcoT14I CCWWGG 2 cut(s) 720, 1218
ErhI CCWWGG 2 cut(s) 720, 1218
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 6 cut(s) 24, 544, 589, 661, 709, 1210
FaqI GGGAC 2 cut(s) 478, 1228
FatI CATG 6 cut(s) 20, 540, 585, 657, 705, 1206
FauI CCCGC 2 cut(s) 49, 1025
FauNDI CATATG 1 cut(s) 1040
FokI GGATG 3 cut(s) 227, 307, 1236
FspBI CTAG 3 cut(s) 1029, 1065, 1085
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 2 cut(s) 369, 443
GsaI CCCAGC 1 cut(s) 978
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 5 cut(s) 25, 725, 746, 1189, 1211
HapII CCGG 3 cut(s) 212, 252, 1212
HgaI GACGC 1 cut(s) 148
HhaI GCGC 2 cut(s) 370, 444
Hin1II CATG 6 cut(s) 24, 544, 589, 661, 709, 1210
Hin6I GCGC 2 cut(s) 368, 442
HinP1I GCGC 2 cut(s) 368, 442
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 4 cut(s) 78, 136, 782, 1229
HpaII CCGG 3 cut(s) 212, 252, 1212
HphI GGTGA 2 cut(s) 57, 310
Hpy166II GTNNAC 2 cut(s) 135, 459
Hpy188I TCNGA 5 cut(s) 147, 274, 631, 1184, 1319
Hpy188III TCNNGA 4 cut(s) 212, 557, 740, 946
Hpy8I GTNNAC 2 cut(s) 135, 459
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 47, 407
HpyCH4III ACNGT 3 cut(s) 985, 1099, 1155
HpyCH4IV ACGT 2 cut(s) 182, 952
HpyF3I CTNAG 3 cut(s) 191, 475, 1316
HpySE526I ACGT 2 cut(s) 182, 952
Hsp92II CATG 6 cut(s) 24, 544, 589, 661, 709, 1210
HspAI GCGC 2 cut(s) 368, 442
Kpn2I TCCGGA 1 cut(s) 211
Kzo9I GATC 3 cut(s) 491, 545, 605
LmnI GCTCC 3 cut(s) 147, 214, 1035
Lsp1109I GCAGC 7 cut(s) 208, 292, 347, 649, 686, 789, 909
LweI GCATC 2 cut(s) 441, 990
MaeI CTAG 3 cut(s) 1029, 1065, 1085
MaeII ACGT 2 cut(s) 182, 952
MaeIII GTNAC 5 cut(s) 16, 27, 183, 1093, 1155
MalI GATC 3 cut(s) 493, 547, 607
MboI GATC 3 cut(s) 491, 545, 605
MboII GAAGA 4 cut(s) 289, 295, 932, 1003
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 2 cut(s) 540, 697
MluCI AATT 8 cut(s) 177, 343, 354, 562, 613, 787, 885, 1269
MlyI GAGTC 2 cut(s) 87, 130
MmeI TCCRAC 2 cut(s) 490, 1194
MnlI CCTC 9 cut(s) 153, 625, 736, 736, 747, 757, 887, 1178, 1325
MroI TCCGGA 1 cut(s) 211
MseI TTAA 3 cut(s) 111, 612, 1148
MspA1I CMGCKG 1 cut(s) 920
MspI CCGG 3 cut(s) 212, 252, 1212
MspR9I CCNGG 2 cut(s) 891, 1213
MvaI CCWGG 1 cut(s) 891
NciI CCSGG 1 cut(s) 1213
NdeI CATATG 1 cut(s) 1040
NdeII GATC 3 cut(s) 491, 545, 605
NheI GCTAGC 1 cut(s) 1028
NlaIII CATG 6 cut(s) 24, 544, 589, 661, 709, 1210
NlaIV GGNNCC 3 cut(s) 149, 537, 1216
NmuCI GTSAC 3 cut(s) 16, 183, 1093
NsbI TGCGCA 1 cut(s) 443
PaqCI CACCTGC 1 cut(s) 979
PceI AGGCCT 1 cut(s) 746
PfeI GAWTC 2 cut(s) 782, 1229
PflMI CCANNNNNTGG 1 cut(s) 1224
PfoI TCCNGGA 1 cut(s) 889
PinAI ACCGGT 1 cut(s) 251
PleI GAGTC 2 cut(s) 86, 130
PpsI GAGTC 2 cut(s) 86, 130
Psp6I CCWGG 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 3 cut(s) 149, 537, 1216
PspPI GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
PstI CTGCAG 1 cut(s) 667
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 605
RsaI GTAC 1 cut(s) 1116
RsaNI GTAC 1 cut(s) 1115
SaqAI TTAA 3 cut(s) 111, 612, 1148
Sau3AI GATC 3 cut(s) 491, 545, 605
Sau96I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
SchI GAGTC 2 cut(s) 87, 130
ScrFI CCNGG 2 cut(s) 891, 1213
SduI GDGCHC 2 cut(s) 540, 697
SfaNI GCATC 2 cut(s) 441, 990
SfcI CTRYAG 1 cut(s) 663
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 5 cut(s) 234, 270, 456, 554, 1215
Sse9I AATT 8 cut(s) 177, 343, 354, 562, 613, 787, 885, 1269
SseBI AGGCCT 1 cut(s) 746
SsiI CCGC 8 cut(s) 42, 151, 237, 454, 918, 1032, 1059, 1203
SspMI CTAG 3 cut(s) 1029, 1065, 1085
StuI AGGCCT 1 cut(s) 746
StyD4I CCNGG 2 cut(s) 889, 1211
StyI CCWWGG 2 cut(s) 720, 1218
TaaI ACNGT 3 cut(s) 985, 1099, 1155
TaiI ACGT 2 cut(s) 185, 955
TaqII GACCGA 1 cut(s) 643
TasI AATT 8 cut(s) 177, 343, 354, 562, 613, 787, 885, 1269
TauI GCSGC 3 cut(s) 153, 920, 1206
TfiI GAWTC 2 cut(s) 782, 1229
Tru1I TTAA 3 cut(s) 111, 612, 1148
Tru9I TTAA 3 cut(s) 111, 612, 1148
TscAI CASTG 4 cut(s) 227, 451, 829, 1023
TseFI GTSAC 3 cut(s) 16, 183, 1093
TseI GCWGC 7 cut(s) 196, 305, 335, 662, 674, 802, 897
Tsp45I GTSAC 3 cut(s) 16, 183, 1093
TspDTI ATGAA 1 cut(s) 694
TspGWI ACGGA 2 cut(s) 87, 794
TspRI CASTG 4 cut(s) 227, 451, 829, 1023
Van91I CCANNNNNTGG 1 cut(s) 1224
VpaK11BI GGWCC 5 cut(s) 234, 270, 456, 554, 1215
XapI RAATTY 1 cut(s) 885
XspI CTAG 3 cut(s) 1029, 1065, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.