Rroxscaffold_4G00280690

Protein of unknown function (DUF819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017890
Physical Location & Seq
Forward (+)
3160982 .. 3163567
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280690.1

Sequence Viewer

Length: 1332 bp
ATGGCTTCCGTCACACTCTCCCTTCCCGCCATACCCACTCCATCACTCTCACTTCAATCTCTCACTAAACCATCCAAGCCCAAACCCGAACTCAGACCCAAACCCAGACCCTTTCCCTCCCATACATTAAACAAAACTTCCCTCCGGCCTTTGACTTCTCCCCACCACCGCCGGTTAAGGGTCACGTCACAGCTCCAGCACCCTATCATCTCTCCGGACGACCACTGGGGTTTATGGACCACCCTCTTCGCCACCGGCGCTTTCGGCCTCTGGTCGGAGAAGACCAAGATTGGTGGGATGGTGAGTGCTGCTTTGGTAAGCACTTTAGTTGGGCTTGCAGCTAGTAATGTAGGGATAATTCCTCATGAAGCGGGTGCTTATTCGATTGTGTTGGAGTTTCTTCTGCCATTATCTATTCCATTGCTGTTGTTTAGAGCAGACTTGCGCAATGTGGCTCGGTCCACTGGGACTTTGCTTCTAGCTTTCCTGCTTGGATCAGTTGCGACTATGGTTGGGACTCTGGTGGCGTTTCTGATGGTGCCTATGCGGTCACTTGGTCAAGATAATTGGAAGATTGCGGCTGCTCTGATGGGTAGTTACATTGGTGGATCTGTTAACTATGTTGCGATTTCAGAGGCTCTTGGTGTTTCCCCATCAGTTATGGCTGCAGGAGTAGCAGCAGACAATGTCATTTGCGCGCTCTACTTCATAGTCTTGTTTGGTTTCGCCTCTAAAATACCTCCTGAGGCCTCAATATCAACTAATGATGTTTCAATGAAAATGGAATCCCAAATTGAAAGGAAACTCCCAGTACTAGAAACTGCAACTGCTCTTGCTACAGCTTTTGTGATATGCAAGGTTTCCACACATCTCACAAGATTATGTGGAATTCAAGGAGGCAACCTGCCCTTAATAACAGCTCTTGTAGTAATTTTAGCTACTCTTCTTCCAACTTATTTCACATACCTTGCACCTTCCGGTGAAACTATTGCTGTAGTCTTGATGCAGGTGTTCTTTGCCGTGCTGGGTGCTACTGGGAGCATATGGAATGTGATGATCACTGCTCCTAGTATATTTGTGTTTGCTTTAGTTCAAGTAACTGTCCATCTAGCTTTTGTCCTGGGATTGGGAAAGCTATTTCACTTTGATTTAAAGCTTTTATTTTTGGCATCAAACGCCAATATTGGAGGGCCAACGACAGCAGGTGGAATGGCCACCGCGAAAGGATGGCATTCTTTGGTTGTTCCGGGAATTCTTGCAGGCATTTTTGGTGTTTCCATTGCAACTTTTCTGGGCATTGGTTTTGGAATCACAGTCCTCAAACACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF819

Protein Analysis

443

Amino Acids

46.74

Weight (kDa)

9.71

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF819 PF05684 81 - 441 1.6e-118 Protein of unknown function (DUF819)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24000
fragaria_vesca FvH4_7g30150
malus_domestica MD01G1207300.v1.1
pyrus_communis pycom01g21820
rosa_chinensis RchiOBHm_Chr1g0378181
rosa_laevigata RLG00000026464
rosa_multiflora Rmu_sc0000625.1_g000011
rosa_roxburghii Rroxscaffold_4G00280690
rosa_rugosa Rorug01G0406800
rosa_samantha Rh1BG385900
rosa_wichuraiana Rw1G037440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 997, 1193
Acc16I TGCGCA 1 cut(s) 446
Acc36I ACCTGC 3 cut(s) 912, 997, 1193
AccB1I GGYRCC 1 cut(s) 538
AccII CGCG 2 cut(s) 698, 1220
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 6 cut(s) 27, 169, 371, 547, 578, 1218
AclWI GGATC 2 cut(s) 502, 616
AcoI YGGCCR 1 cut(s) 1212
AcsI RAATTY 2 cut(s) 888, 1251
AfaI GTAC 1 cut(s) 813
AfiI CCNNNNNNNGG 3 cut(s) 178, 274, 1126
AgsI TTSAA 5 cut(s) 56, 774, 797, 893, 1094
AjiI CACGTC 1 cut(s) 186
AjnI CCWGG 1 cut(s) 1119
AluBI AGCT 9 cut(s) 193, 341, 482, 842, 920, 938, 1112, 1135, 1156
AluI AGCT 9 cut(s) 193, 341, 482, 842, 920, 938, 1112, 1135, 1156
AlwI GGATC 2 cut(s) 502, 616
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 5 cut(s) 146, 265, 747, 1190, 1212
ApeKI GCWGC 5 cut(s) 308, 338, 581, 665, 677
ApoI RAATTY 2 cut(s) 888, 1251
AspLEI GCGC 4 cut(s) 260, 447, 698, 700
AspS9I GGNCC 3 cut(s) 237, 459, 1190
AsuC2I CCSGG 1 cut(s) 1248
AsuHPI GGTGA 2 cut(s) 313, 992
AvaII GGWCC 2 cut(s) 237, 459
AxyI CCTNAGG 1 cut(s) 744
BalI TGGCCA 1 cut(s) 1214
BanI GGYRCC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 5 cut(s) 295, 350, 568, 652, 689
BccI CCATC 8 cut(s) 49, 79, 292, 529, 583, 661, 1113, 1221
BceAI ACGGC 1 cut(s) 1004
BciT130I CCWGG 1 cut(s) 1121
BclI TGATCA 1 cut(s) 1056
BcnI CCSGG 1 cut(s) 1248
BfaI CTAG 5 cut(s) 342, 479, 815, 1068, 1109
BfmI CTRYAG 3 cut(s) 666, 837, 993
BfoI RGCGCY 1 cut(s) 261
BfuAI ACCTGC 3 cut(s) 912, 997, 1193
BisI GCNGC 6 cut(s) 309, 339, 579, 582, 666, 678
BlsI GCNGC 6 cut(s) 310, 340, 580, 583, 667, 679
BmcAI AGTACT 1 cut(s) 813
Bme1390I CCNGG 2 cut(s) 1121, 1248
Bme18I GGWCC 2 cut(s) 237, 459
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 3 cut(s) 237, 459, 1190
BmiI GGNNCC 1 cut(s) 540
BmrFI CCNGG 2 cut(s) 1121, 1248
BmrI ACTGGG 4 cut(s) 235, 474, 803, 1044
BmsI GCATC 2 cut(s) 993, 1178
BmuI ACTGGG 4 cut(s) 235, 474, 803, 1044
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 1248
BsaJI CCNNGG 1 cut(s) 1120
BsaWI WCCGGW 2 cut(s) 214, 977
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 274, 1126
Bse118I RCCGGY 2 cut(s) 171, 254
Bse1I ACTGG 4 cut(s) 230, 469, 809, 1039
Bse21I CCTNAGG 1 cut(s) 744
Bse3DI GCAATG 3 cut(s) 419, 454, 1278
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 1 cut(s) 1121
BseDI CCNNGG 1 cut(s) 1120
BseGI GGATG 3 cut(s) 71, 303, 1232
BseLI CCNNNNNNNGG 3 cut(s) 178, 274, 1126
BseMI GCAATG 3 cut(s) 419, 454, 1278
BseMII CTCAG 2 cut(s) 106, 735
BseNI ACTGG 4 cut(s) 230, 469, 809, 1039
BsePI GCGCGC 1 cut(s) 696
BseXI GCAGC 5 cut(s) 295, 350, 568, 652, 689
BseYI CCCAGC 1 cut(s) 1024
Bsh1236I CGCG 2 cut(s) 698, 1220
BshFI GGCC 5 cut(s) 148, 267, 749, 1192, 1214
BshNI GGYRCC 1 cut(s) 538
BsiSI CCGG 6 cut(s) 145, 172, 215, 255, 978, 1247
BslFI GGGAC 2 cut(s) 481, 529
BslI CCNNNNNNNGG 3 cut(s) 178, 274, 1126
BsmFI GGGAC 2 cut(s) 481, 529
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 5 cut(s) 148, 267, 749, 1192, 1214
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 3 cut(s) 494, 608, 1056
BspACI CCGC 6 cut(s) 27, 169, 371, 547, 578, 1218
BspANI GGCC 5 cut(s) 148, 267, 749, 1192, 1214
BspCNI CTCAG 2 cut(s) 105, 736
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 2 cut(s) 698, 1220
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 540
BspMAI CTGCAG 1 cut(s) 670
BspMI ACCTGC 3 cut(s) 912, 997, 1193
BspPI GGATC 2 cut(s) 502, 616
BspT107I GGYRCC 1 cut(s) 538
BsrDI GCAATG 3 cut(s) 419, 454, 1278
BsrFI RCCGGY 2 cut(s) 171, 254
BsrI ACTGG 4 cut(s) 230, 469, 809, 1039
BssAI RCCGGY 2 cut(s) 171, 254
BssECI CCNNGG 1 cut(s) 1120
BssHII GCGCGC 1 cut(s) 696
BssMI GATC 3 cut(s) 494, 608, 1056
Bst2UI CCWGG 1 cut(s) 1121
Bst4CI ACNGT 2 cut(s) 1102, 1315
Bst6I CTCTTC 2 cut(s) 251, 948
BstC8I GCNNGC 3 cut(s) 336, 698, 1261
BstDEI CTNAG 2 cut(s) 92, 744
BstF5I GGATG 3 cut(s) 71, 303, 1232
BstFNI CGCG 2 cut(s) 698, 1220
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 4 cut(s) 260, 447, 698, 700
BstKTI GATC 3 cut(s) 497, 611, 1059
BstMBI GATC 3 cut(s) 494, 608, 1056
BstMWI GCNNNNNNNGC 4 cut(s) 257, 264, 674, 1175
BstNI CCWGG 1 cut(s) 1121
BstSCI CCNGG 2 cut(s) 1119, 1246
BstSFI CTRYAG 3 cut(s) 666, 837, 993
BstUI CGCG 2 cut(s) 698, 1220
BstV1I GCAGC 5 cut(s) 295, 350, 568, 652, 689
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 608
BstYI RGATCY 1 cut(s) 608
Bsu36I CCTNAGG 1 cut(s) 744
BsuRI GGCC 5 cut(s) 148, 267, 749, 1192, 1214
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 3 cut(s) 71, 303, 1232
BtsI GCAGTG 1 cut(s) 1059
BtsIMutI CAGTG 3 cut(s) 223, 462, 1059
BveI ACCTGC 3 cut(s) 912, 997, 1193
Cac8I GCNNGC 3 cut(s) 336, 698, 1261
CciI TCATGA 1 cut(s) 364
CfoI GCGC 4 cut(s) 260, 447, 698, 700
Cfr10I RCCGGY 2 cut(s) 171, 254
Cfr13I GGNCC 3 cut(s) 237, 459, 1190
Csp6I GTAC 1 cut(s) 812
CspCI CAANNNNNGTGG 2 cut(s) 274, 309
CviAII CATG 1 cut(s) 365
CviQI GTAC 1 cut(s) 812
DdeI CTNAG 2 cut(s) 92, 744
DpnI GATC 3 cut(s) 496, 610, 1058
DpnII GATC 3 cut(s) 494, 608, 1056
DraI TTTAAA 1 cut(s) 1152
EaeI YGGCCR 1 cut(s) 1212
Eam1104I CTCTTC 2 cut(s) 251, 948
EarI CTCTTC 2 cut(s) 251, 948
Eco147I AGGCCT 1 cut(s) 749
Eco47I GGWCC 2 cut(s) 237, 459
Eco81I CCTNAGG 1 cut(s) 744
EcoRI GAATTC 2 cut(s) 888, 1251
EcoRII CCWGG 1 cut(s) 1119
FaeI CATG 1 cut(s) 368
FaqI GGGAC 2 cut(s) 481, 529
FatI CATG 1 cut(s) 364
FauI CCCGC 2 cut(s) 34, 364
FauNDI CATATG 1 cut(s) 1043
FbaI TGATCA 1 cut(s) 1056
Fnu4HI GCNGC 6 cut(s) 309, 339, 579, 582, 666, 678
FokI GGATG 3 cut(s) 58, 310, 1239
Fsp4HI GCNGC 6 cut(s) 309, 339, 579, 582, 666, 678
FspBI CTAG 5 cut(s) 342, 479, 815, 1068, 1109
FspI TGCGCA 1 cut(s) 446
GlaI GCGC 4 cut(s) 259, 446, 697, 699
GluI GCNGC 6 cut(s) 309, 339, 579, 582, 666, 678
GsaI CCCAGC 1 cut(s) 1028
GsuI CTGGAG 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 5 cut(s) 148, 267, 749, 1192, 1214
HapII CCGG 6 cut(s) 145, 172, 215, 255, 978, 1247
HhaI GCGC 4 cut(s) 260, 447, 698, 700
Hin1II CATG 1 cut(s) 368
Hin6I GCGC 4 cut(s) 258, 445, 696, 698
HinP1I GCGC 4 cut(s) 258, 445, 696, 698
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HindIII AAGCTT 1 cut(s) 1154
HinfI GANTC 3 cut(s) 517, 785, 1308
HpaI GTTAAC 1 cut(s) 616
HpaII CCGG 6 cut(s) 145, 172, 215, 255, 978, 1247
HphI GGTGA 2 cut(s) 313, 992
Hpy166II GTNNAC 2 cut(s) 462, 616
Hpy188I TCNGA 5 cut(s) 95, 277, 534, 588, 634
Hpy188III TCNNGA 5 cut(s) 215, 365, 560, 743, 1000
Hpy8I GTNNAC 2 cut(s) 462, 616
HpyAV CCTTC 2 cut(s) 32, 984
HpyCH4III ACNGT 2 cut(s) 1102, 1315
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 8 cut(s) 338, 668, 824, 855, 971, 1006, 1259, 1283
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 264, 674, 1175
HpyF3I CTNAG 2 cut(s) 92, 744
HpySE526I ACGT 1 cut(s) 185
Hsp92II CATG 1 cut(s) 368
HspAI GCGC 4 cut(s) 258, 445, 696, 698
Kpn2I TCCGGA 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 1056
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 3 cut(s) 494, 608, 1056
LmnI GCTCC 3 cut(s) 198, 1038, 1069
Lsp1109I GCAGC 5 cut(s) 295, 350, 568, 652, 689
LweI GCATC 2 cut(s) 993, 1178
MaeI CTAG 5 cut(s) 342, 479, 815, 1068, 1109
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 6 cut(s) 10, 181, 186, 549, 596, 1096
MalI GATC 3 cut(s) 496, 610, 1058
MboI GATC 3 cut(s) 494, 608, 1056
MboII GAAGA 6 cut(s) 238, 292, 392, 583, 935, 938
MflI RGATCY 1 cut(s) 608
MlsI TGGCCA 1 cut(s) 1214
MluCI AATT 6 cut(s) 357, 565, 792, 888, 930, 1251
MluNI TGGCCA 1 cut(s) 1214
MlyI GAGTC 1 cut(s) 511
MmeI TCCRAC 3 cut(s) 255, 372, 974
Mox20I TGGCCA 1 cut(s) 1214
MroI TCCGGA 1 cut(s) 214
MscI TGGCCA 1 cut(s) 1214
MseI TTAA 5 cut(s) 128, 176, 615, 911, 1151
Msp20I TGGCCA 1 cut(s) 1214
MspI CCGG 6 cut(s) 145, 172, 215, 255, 978, 1247
MspR9I CCNGG 2 cut(s) 1121, 1248
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 1 cut(s) 1121
MvnI CGCG 2 cut(s) 698, 1220
MwoI GCNNNNNNNGC 4 cut(s) 257, 264, 674, 1175
NciI CCSGG 1 cut(s) 1248
NdeI CATATG 1 cut(s) 1043
NdeII GATC 3 cut(s) 494, 608, 1056
NlaIII CATG 1 cut(s) 368
NlaIV GGNNCC 1 cut(s) 540
NmuCI GTSAC 4 cut(s) 10, 181, 186, 549
NsbI TGCGCA 1 cut(s) 446
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 2 cut(s) 997, 1193
PauI GCGCGC 1 cut(s) 696
PceI AGGCCT 1 cut(s) 749
PctI GAATGC 1 cut(s) 1231
PfeI GAWTC 2 cut(s) 785, 1308
PflFI GACNNNGTC 1 cut(s) 686
PfoI TCCNGGA 1 cut(s) 1246
PkrI GCNGC 6 cut(s) 310, 340, 580, 583, 667, 679
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 1119
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 1 cut(s) 1119
PspN4I GGNNCC 1 cut(s) 540
PspPI GGNCC 3 cut(s) 237, 459, 1190
PstI CTGCAG 1 cut(s) 670
PsuI RGATCY 1 cut(s) 608
PsyI GACNNNGTC 1 cut(s) 686
PteI GCGCGC 1 cut(s) 696
RsaI GTAC 1 cut(s) 813
RsaNI GTAC 1 cut(s) 812
SaqAI TTAA 5 cut(s) 128, 176, 615, 911, 1151
SatI GCNGC 6 cut(s) 309, 339, 579, 582, 666, 678
Sau3AI GATC 3 cut(s) 494, 608, 1056
Sau96I GGNCC 3 cut(s) 237, 459, 1190
ScaI AGTACT 1 cut(s) 813
SchI GAGTC 1 cut(s) 511
ScrFI CCNGG 2 cut(s) 1121, 1248
SfaNI GCATC 2 cut(s) 993, 1178
SfcI CTRYAG 3 cut(s) 666, 837, 993
SgrAI CRCCGGYG 1 cut(s) 254
SinI GGWCC 2 cut(s) 237, 459
Sse9I AATT 6 cut(s) 357, 565, 792, 888, 930, 1251
SseBI AGGCCT 1 cut(s) 749
SsiI CCGC 6 cut(s) 27, 169, 371, 547, 578, 1218
SspI AATATT 1 cut(s) 1183
SspMI CTAG 5 cut(s) 342, 479, 815, 1068, 1109
StuI AGGCCT 1 cut(s) 749
StyD4I CCNGG 2 cut(s) 1119, 1246
TaaI ACNGT 2 cut(s) 1102, 1315
TaiI ACGT 1 cut(s) 188
TaqI TCGA 1 cut(s) 383
TaqII GACCGA 1 cut(s) 447
TasI AATT 6 cut(s) 357, 565, 792, 888, 930, 1251
TatI WGTACW 1 cut(s) 811
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 2 cut(s) 785, 1308
Tru1I TTAA 5 cut(s) 128, 176, 615, 911, 1151
Tru9I TTAA 5 cut(s) 128, 176, 615, 911, 1151
TscAI CASTG 3 cut(s) 230, 469, 1066
TseFI GTSAC 4 cut(s) 10, 181, 186, 549
TseI GCWGC 5 cut(s) 308, 338, 581, 665, 677
Tsp45I GTSAC 4 cut(s) 10, 181, 186, 549
TspDTI ATGAA 3 cut(s) 381, 697, 791
TspRI CASTG 3 cut(s) 230, 469, 1066
Tth111I GACNNNGTC 1 cut(s) 686
VpaK11BI GGWCC 2 cut(s) 237, 459
XapI RAATTY 2 cut(s) 888, 1251
XspI CTAG 5 cut(s) 342, 479, 815, 1068, 1109
ZrmI AGTACT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.