pycom01g21820

Protein of unknown function (DUF819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0017890
Physical Location & Seq
Reverse (-)
19675409 .. 19678075
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21820.6

Sequence Viewer

Length: 1329 bp
ATGGCTTCTCTCCCCGTCACATGGCCATTACTCCACCTTCCCGCCACTCCCACTCCATCACTCTCACCAGTCAAACGAGTCAAACCCAACCCCTTTCCGTCTCACGCCGTTAATGGGACGCTTCTCTTGCCGTTGACTCGTCCTCGGAGCCGCAAATGCGTCCAAGCGACAAGGGTAATTACGTCACAGCTCAGGCAGCCTATCGTCGCTACCGATGACCATTGGGGCTTATGGACCGCCCTGTTCGCCACCGGTGCTTTTGGCCTCTGGTCCGAGAAGACGAAGATTGGTGGAATGGTGAGTGCTGCCCTTGTGAGCACTTTGGTTGGGCTTGCAGCAAGTAATTTTGGCATAATTCCCTTTGAAGCGCCTGCATATTCCATTGTCTTGGAGTTCCTTCTACCATTATCTATTCCATTGCTATTATATAGAGCTGACTTGCGCAGTGTGATGCGGTCCACAGGGACTTTGCTCTTAGCTTTCGTGCTTGGATCAGTTGCGACTATGGTTGGAACTCTCGTGGCATTTCAAATGGTGCCCATCCGATCACTTGGTCCAGATAATTGGAAAATAGCTTCTGCTCTCATGGGTAGCTATATTGGTGGATCTGTTAATTATGTTGCGATTTCAGAGGCTCTTGGTGTTTCTCCATCAGTCGTGGCTGCAGGAGTAGCAGTAGACAATGTTATTTGTGCTCTGTATTTCATGGTGTTGTTTGCTTTGGCCTCTAAAATACCTCCCGAGGCCTCAACATCAACTGATGATGTTTCAATGCAAACGGAATCCAATTACGACAGAAAGCTGCCAGTTGTAGAAACTGCCACTGCTCTTGCTACATCGTTTGTGATATGCAAGGCTTCCACATATCTTACAGGATTGTGTGGAATTCCAGGAGGCAGCTTGCCAATGATAACAGCCACTGTTGTCATATTAGCAACTCTTCTCCCGACACATTTCGCTTACCTTGCACCTGCTGGGGATACCGTTGCTTTGCTTTTGATGCAGGTATTCTTCGCAGTGTTGGGGGCTAGCGGGAGCATATGGAATGTCATTAACACCGCGCCTAGTATCTTTTTCTTTGCTCTAGTTCAAGTCACCGTCCATCTTGCCGTGGTGCTTGGATTGGGAAAACTATTTCGCTTAGATTTAAAACTGTTACTTTTGGCGTCCAACGCCAATATCGGAGGGCCAACGACAGCAGGCGGAATGGCCGGGACCAAAGGATGGGATTCTTTGGTTGTTCCTGCAATCCTTGCAGGCATTTTTGGAGTTGCCATTGCAACATTTCTTGGCATTGGTTTTGGAATGATAGTCCTCAGACACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF819

Protein Analysis

443

Amino Acids

46.27

Weight (kDa)

9.3

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24000
fragaria_vesca FvH4_7g30150
malus_domestica MD01G1207300.v1.1
pyrus_communis pycom01g21820
rosa_chinensis RchiOBHm_Chr1g0378181
rosa_laevigata RLG00000026464
rosa_multiflora Rmu_sc0000625.1_g000011
rosa_roxburghii Rroxscaffold_4G00280690
rosa_rugosa Rorug01G0406800
rosa_samantha Rh1BG385900
rosa_wichuraiana Rw1G037440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 979
Acc16I TGCGCA 1 cut(s) 443
Acc36I ACCTGC 2 cut(s) 979, 994
AccB1I GGYRCC 1 cut(s) 535
AccB7I CCANNNNNTGG 1 cut(s) 1224
AccI GTMKAC 1 cut(s) 678
AccII CGCG 1 cut(s) 1061
AciI CCGC 7 cut(s) 42, 151, 237, 454, 1032, 1059, 1203
AclWI GGATC 2 cut(s) 499, 613
AcoI YGGCCR 2 cut(s) 23, 1209
AcsI RAATTY 1 cut(s) 885
AcyI GRCGYC 1 cut(s) 1166
AfiI CCNNNNNNNGG 3 cut(s) 21, 114, 1224
AgeI ACCGGT 1 cut(s) 251
AgsI TTSAA 4 cut(s) 365, 530, 771, 1091
AjnI CCWGG 1 cut(s) 889
AluBI AGCT 7 cut(s) 190, 434, 479, 575, 594, 802, 900
AluI AGCT 7 cut(s) 190, 434, 479, 575, 594, 802, 900
Alw21I GWGCWC 2 cut(s) 320, 697
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 2 cut(s) 499, 613
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 1 cut(s) 740
AoxI GGCC 6 cut(s) 23, 262, 723, 744, 1187, 1209
ApeKI GCWGC 6 cut(s) 196, 305, 335, 662, 802, 897
ApoI RAATTY 1 cut(s) 885
AsiGI ACCGGT 1 cut(s) 251
AspLEI GCGC 3 cut(s) 370, 444, 1063
AspS9I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
AsuC2I CCSGG 1 cut(s) 1213
AsuHPI GGTGA 3 cut(s) 57, 310, 1087
AsuNHI GCTAGC 1 cut(s) 1028
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 5 cut(s) 234, 270, 456, 554, 1215
BaeGI GKGCMC 1 cut(s) 540
BalI TGGCCA 1 cut(s) 25
BanI GGYRCC 1 cut(s) 535
BauI CACGAG 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 2 cut(s) 320, 697
BbvI GCAGC 6 cut(s) 208, 292, 347, 649, 789, 909
BccI CCATC 5 cut(s) 64, 548, 658, 1110, 1218
BceAI ACGGC 3 cut(s) 92, 115, 1094
BciT130I CCWGG 1 cut(s) 891
BciVI GTATCC 1 cut(s) 973
BcnI CCSGG 1 cut(s) 1213
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 3 cut(s) 1029, 1065, 1085
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 371
BfuAI ACCTGC 2 cut(s) 979, 994
BfuI GTATCC 1 cut(s) 973
BisI GCNGC 7 cut(s) 151, 197, 306, 336, 663, 803, 898
BlsI GCNGC 7 cut(s) 152, 198, 307, 337, 664, 804, 899
Bme1390I CCNGG 2 cut(s) 891, 1213
Bme18I GGWCC 5 cut(s) 234, 270, 456, 554, 1215
BmeT110I CYCGRG 1 cut(s) 740
BmgT120I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
BmiI GGNNCC 3 cut(s) 149, 537, 1216
BmrFI CCNGG 2 cut(s) 891, 1213
BmsI GCATC 2 cut(s) 441, 990
BmtI GCTAGC 1 cut(s) 1032
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 1213
BsaHI GRCGYC 1 cut(s) 1166
BsaJI CCNNGG 3 cut(s) 143, 741, 1110
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 4 cut(s) 37, 67, 660, 690
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 114, 1224
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 68, 806
Bse3DI GCAATG 2 cut(s) 416, 1275
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 3 cut(s) 143, 741, 1110
BseGI GGATG 2 cut(s) 540, 1229
BseLI CCNNNNNNNGG 3 cut(s) 21, 114, 1224
BseMI GCAATG 2 cut(s) 416, 1275
BseMII CTCAG 1 cut(s) 205
BseNI ACTGG 2 cut(s) 68, 806
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 6 cut(s) 208, 292, 347, 649, 789, 909
BseYI CCCAGC 1 cut(s) 974
Bsh1236I CGCG 1 cut(s) 1061
BshFI GGCC 6 cut(s) 25, 264, 725, 746, 1189, 1211
BshNI GGYRCC 1 cut(s) 535
BshTI ACCGGT 1 cut(s) 251
BsiHKAI GWGCWC 2 cut(s) 320, 697
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 2 cut(s) 252, 1212
BslFI GGGAC 3 cut(s) 130, 478, 1228
BslI CCNNNNNNNGG 3 cut(s) 21, 114, 1224
BsmAI GTCTC 1 cut(s) 105
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 3 cut(s) 130, 478, 1228
BsnI GGCC 6 cut(s) 25, 264, 725, 746, 1189, 1211
BsoBI CYCGRG 1 cut(s) 740
Bsp1286I GDGCHC 3 cut(s) 320, 540, 697
Bsp143I GATC 3 cut(s) 491, 545, 605
BspACI CCGC 7 cut(s) 42, 151, 237, 454, 1032, 1059, 1203
BspANI GGCC 6 cut(s) 25, 264, 725, 746, 1189, 1211
BspCNI CTCAG 2 cut(s) 204, 1329
BspFNI CGCG 1 cut(s) 1061
BspLI GGNNCC 3 cut(s) 149, 537, 1216
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 2 cut(s) 979, 994
BspOI GCTAGC 1 cut(s) 1032
BspPI GGATC 2 cut(s) 499, 613
BspT107I GGYRCC 1 cut(s) 535
BsrDI GCAATG 2 cut(s) 416, 1275
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 2 cut(s) 68, 806
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 3 cut(s) 143, 741, 1110
BssMI GATC 3 cut(s) 491, 545, 605
BssNI GRCGYC 1 cut(s) 1166
BssSI CACGAG 1 cut(s) 518
Bst2BI CACGAG 1 cut(s) 518
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 4 cut(s) 922, 985, 1099, 1155
Bst6I CTCTTC 1 cut(s) 945
BstACI GRCGYC 1 cut(s) 1166
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstC8I GCNNGC 6 cut(s) 333, 372, 902, 1030, 1201, 1258
BstDEI CTNAG 4 cut(s) 191, 475, 1141, 1316
BstDSI CCRYGG 1 cut(s) 1110
BstF5I GGATG 2 cut(s) 540, 1229
BstFNI CGCG 1 cut(s) 1061
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 3 cut(s) 370, 444, 1063
BstKTI GATC 3 cut(s) 494, 548, 608
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 3 cut(s) 491, 545, 605
BstNI CCWGG 1 cut(s) 891
BstSCI CCNGG 2 cut(s) 889, 1211
BstSFI CTRYAG 1 cut(s) 663
BstSLI GKGCMC 1 cut(s) 540
BstUI CGCG 1 cut(s) 1061
BstV1I GCAGC 6 cut(s) 208, 292, 347, 649, 789, 909
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 605
BstXI CCANNNNNNTGG 2 cut(s) 388, 564
BstYI RGATCY 1 cut(s) 605
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 6 cut(s) 25, 264, 725, 746, 1189, 1211
BtgI CCRYGG 1 cut(s) 1110
BtsCI GGATG 2 cut(s) 540, 1229
BtsI GCAGTG 3 cut(s) 451, 822, 1023
BtsIMutI CAGTG 4 cut(s) 451, 822, 918, 1023
BveI ACCTGC 2 cut(s) 979, 994
Cac8I GCNNGC 6 cut(s) 333, 372, 902, 1030, 1201, 1258
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 3 cut(s) 370, 444, 1063
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
CseI GACGC 3 cut(s) 127, 148, 1155
CspAI ACCGGT 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 811, 846
CviAII CATG 3 cut(s) 21, 586, 706
DdeI CTNAG 4 cut(s) 191, 475, 1141, 1316
DpnI GATC 3 cut(s) 493, 547, 607
DpnII GATC 3 cut(s) 491, 545, 605
DraI TTTAAA 1 cut(s) 1149
EaeI YGGCCR 2 cut(s) 23, 1209
Eam1104I CTCTTC 1 cut(s) 945
EarI CTCTTC 1 cut(s) 945
EciI GGCGGA 1 cut(s) 1218
Eco147I AGGCCT 1 cut(s) 746
Eco47I GGWCC 5 cut(s) 234, 270, 456, 554, 1215
Eco88I CYCGRG 1 cut(s) 740
EcoRI GAATTC 1 cut(s) 885
EcoRII CCWGG 1 cut(s) 889
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 3 cut(s) 24, 589, 709
FaqI GGGAC 3 cut(s) 130, 478, 1228
FatI CATG 3 cut(s) 20, 585, 705
FauI CCCGC 2 cut(s) 49, 1025
FauNDI CATATG 1 cut(s) 1040
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 7 cut(s) 151, 197, 306, 336, 663, 803, 898
FokI GGATG 2 cut(s) 527, 1236
Fsp4HI GCNGC 7 cut(s) 151, 197, 306, 336, 663, 803, 898
FspBI CTAG 3 cut(s) 1029, 1065, 1085
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 3 cut(s) 369, 443, 1062
GluI GCNGC 7 cut(s) 151, 197, 306, 336, 663, 803, 898
GsaI CCCAGC 1 cut(s) 978
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 6 cut(s) 25, 264, 725, 746, 1189, 1211
HapII CCGG 2 cut(s) 252, 1212
HgaI GACGC 3 cut(s) 127, 148, 1155
HhaI GCGC 3 cut(s) 370, 444, 1063
Hin1I GRCGYC 1 cut(s) 1166
Hin1II CATG 3 cut(s) 24, 589, 709
Hin6I GCGC 3 cut(s) 368, 442, 1061
HinP1I GCGC 3 cut(s) 368, 442, 1061
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 4 cut(s) 78, 136, 782, 1229
HpaII CCGG 2 cut(s) 252, 1212
HphI GGTGA 3 cut(s) 57, 310, 1087
Hpy166II GTNNAC 3 cut(s) 135, 459, 679
Hpy188I TCNGA 6 cut(s) 147, 274, 545, 631, 1184, 1319
Hpy188III TCNNGA 3 cut(s) 557, 740, 946
Hpy8I GTNNAC 3 cut(s) 135, 459, 679
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 47, 407
HpyCH4III ACNGT 4 cut(s) 922, 985, 1099, 1155
HpyCH4IV ACGT 1 cut(s) 182
HpyF3I CTNAG 4 cut(s) 191, 475, 1141, 1316
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 1166
Hsp92II CATG 3 cut(s) 24, 589, 709
HspAI GCGC 3 cut(s) 368, 442, 1061
Kzo9I GATC 3 cut(s) 491, 545, 605
LmnI GCTCC 2 cut(s) 147, 1035
Lsp1109I GCAGC 6 cut(s) 208, 292, 347, 649, 789, 909
LweI GCATC 2 cut(s) 441, 990
MaeI CTAG 3 cut(s) 1029, 1065, 1085
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 4 cut(s) 16, 183, 1093, 1155
MalI GATC 3 cut(s) 493, 547, 607
MboI GATC 3 cut(s) 491, 545, 605
MboII GAAGA 4 cut(s) 289, 295, 932, 1003
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 3 cut(s) 320, 540, 697
MlsI TGGCCA 1 cut(s) 25
MluCI AATT 7 cut(s) 177, 343, 354, 562, 613, 787, 885
MluNI TGGCCA 1 cut(s) 25
MlyI GAGTC 2 cut(s) 87, 130
MmeI TCCRAC 2 cut(s) 490, 1194
Mox20I TGGCCA 1 cut(s) 25
MscI TGGCCA 1 cut(s) 25
MseI TTAA 4 cut(s) 111, 612, 1053, 1148
Msp20I TGGCCA 1 cut(s) 25
MspI CCGG 2 cut(s) 252, 1212
MspR9I CCNGG 2 cut(s) 891, 1213
MvaI CCWGG 1 cut(s) 891
MvnI CGCG 1 cut(s) 1061
NciI CCSGG 1 cut(s) 1213
NdeI CATATG 1 cut(s) 1040
NdeII GATC 3 cut(s) 491, 545, 605
NheI GCTAGC 1 cut(s) 1028
NlaIII CATG 3 cut(s) 24, 589, 709
NlaIV GGNNCC 3 cut(s) 149, 537, 1216
NmuCI GTSAC 3 cut(s) 16, 183, 1093
NsbI TGCGCA 1 cut(s) 443
PaqCI CACCTGC 1 cut(s) 979
PceI AGGCCT 1 cut(s) 746
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 2 cut(s) 782, 1229
PflMI CCANNNNNTGG 1 cut(s) 1224
PfoI TCCNGGA 1 cut(s) 889
PinAI ACCGGT 1 cut(s) 251
PkrI GCNGC 7 cut(s) 152, 198, 307, 337, 664, 804, 899
PleI GAGTC 2 cut(s) 86, 130
PpsI GAGTC 2 cut(s) 86, 130
Psp6I CCWGG 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 3 cut(s) 149, 537, 1216
PspPI GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
PstI CTGCAG 1 cut(s) 667
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 605
SaqAI TTAA 4 cut(s) 111, 612, 1053, 1148
SatI GCNGC 7 cut(s) 151, 197, 306, 336, 663, 803, 898
Sau3AI GATC 3 cut(s) 491, 545, 605
Sau96I GGNCC 6 cut(s) 234, 270, 456, 554, 1187, 1215
SchI GAGTC 2 cut(s) 87, 130
ScrFI CCNGG 2 cut(s) 891, 1213
SduI GDGCHC 3 cut(s) 320, 540, 697
SfaNI GCATC 2 cut(s) 441, 990
SfcI CTRYAG 1 cut(s) 663
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 5 cut(s) 234, 270, 456, 554, 1215
Sse9I AATT 7 cut(s) 177, 343, 354, 562, 613, 787, 885
SseBI AGGCCT 1 cut(s) 746
SsiI CCGC 7 cut(s) 42, 151, 237, 454, 1032, 1059, 1203
SspMI CTAG 3 cut(s) 1029, 1065, 1085
StuI AGGCCT 1 cut(s) 746
StyD4I CCNGG 2 cut(s) 889, 1211
TaaI ACNGT 4 cut(s) 922, 985, 1099, 1155
TaiI ACGT 1 cut(s) 185
TasI AATT 7 cut(s) 177, 343, 354, 562, 613, 787, 885
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 782, 1229
Tru1I TTAA 4 cut(s) 111, 612, 1053, 1148
Tru9I TTAA 4 cut(s) 111, 612, 1053, 1148
TscAI CASTG 4 cut(s) 451, 829, 925, 1023
TseFI GTSAC 3 cut(s) 16, 183, 1093
TseI GCWGC 6 cut(s) 196, 305, 335, 662, 802, 897
Tsp45I GTSAC 3 cut(s) 16, 183, 1093
TspDTI ATGAA 1 cut(s) 694
TspGWI ACGGA 2 cut(s) 87, 794
TspRI CASTG 4 cut(s) 451, 829, 925, 1023
Van91I CCANNNNNTGG 1 cut(s) 1224
VpaK11BI GGWCC 5 cut(s) 234, 270, 456, 554, 1215
XapI RAATTY 1 cut(s) 885
XmiI GTMKAC 1 cut(s) 678
XspI CTAG 3 cut(s) 1029, 1065, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.