Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012339

Physical Location & Seq

Genomic Coordinates Reverse (-)

30399445 .. 30403736

Gene Structure 4292 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1210000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGGCTGCTTCGGAAGAGAATAGTGTGTTGTTCCCAATTTTCATTTTGACCATAATGGCACTGCCTCTAGTGCCTTATACAATTACGAAGTTATGTCGTGCTGCATCCAAGAAAGCAAAGAGCATCCACTGTCAGTGCTCTGAGTGCGCTCGGTCAGGAAAGTACCGCAAGTCAATTTTTAAGCGGATTTCAAACTTCTCAACATGGAGTAACTTGACATTAATACTTCTCTGGGTTATCATGATAGTCCTGGTTTATTACATCAAAAATATGAGCCGCGAGATTCAAGTTTTCGAGCCATTCAGTATTCTTGGACTGGAACCTGGAGCTTCTGATTCAGAAATAAAGAAGGCATATAGGAGACTCTCCATACAGTACCATCCAGATAAAAATCCAGATCCAGAGGCCCACAATTACTTTGTGGAGTTCATATCAAAGGCTTATCAGGCTCTGATGGATCCAGTATCTCGTGAGAATTTTGAAAAATATGGCCATCCAGATGGCAGGCAGGGGTTTCAAATGGGCATAGCTCTTCCTCAGTTCCTGCTACACATCGATGGGGCATCTAGTGGAATACTCCTACTCTGGATTGTTGGTATTTGTATTCTATTGCCATTGGTGATAGCTGTTGTATATCTTTCAAGATCGGCAAAATATACCGGCAACTATGTCATGCATCACACATTATCCACTTACGATTACTGCATGAAGCCTTCTCTGGCTCCCAGCAAAGTAATGGATGTCTTCATAAAGGCTGCTGAATATACTGAAATTCCGGTTCGCAGAACTGACGATGAACCCCTTCAGAAGCTTTTTATGCTAGTCAGGAGTGAGTTGAATCTGGACCTTAAGAATATCAAGCAAGAGCAGGCAAAGTTTTGGAAACAGCATCCAGCCCTTGTAAAGACAGAATTGTTGATTCAAGCACAGTTAACTCGTGAGTCAGCATCCTTATCTCCATCTTTGCAAGGTGATTTTAGACGTGTTCTGGAACTTGCCCCTTGCCTCCTTGAAGAATTGATGAAGATAGCAGTTATGCCACGCAATGCCCAGGGGCAGGGATGGCTGAGGCCTGCTATTGGTGTAGTTGAACTTTCTCAGTGTATCATTCAGGCTGTTCCTCTTAGTGCAAGGAAGGCAATTGGAGGATCCAGTGAAGGCATTGCCCCCTTTCTGCAGCTGCCACATTTTAGTGAGGCGGTTATAAAAAAGATAGCCCGCAAGAAGCTGAGAGTGTTTCAGGAGCTTCAGGACATGTCCGTGGAAGATCGTGCTGAGGTACTTTCTCAAACAGCTGGATTCTCATCCGCTGAAGTCCAGGATGTTGAAATGGTATTGCAGACGATGCCTTCGATATCAATTGAAGTTCCAGATGAGACTGAAGGTAAAGATGGTATACAAGAGGGTGACATTGTCACGGTTCATGCTTGGGTCACACTAAAGCGTGCCAACGGACTGATTGGTGCCCTTCCCCACGCCCCATACTTCCCGTTTCACAAGGAAGAAAACTTCTGGTTTTTGCTTGCAGACAGTGTCTCCAATAACGTTTGGTTTTGGCAGAAGGTAAATTTCATGGATGAAGCTGCAGCCATTACTGTTGCTTCCAAGGCAATCGAGGACACAAAGGAGGGTTCGGGAGCAACTATGAAAGAGATTAGTGCTGCTGTCAAAGAAGCAGTCGAGAAGGTGAAGAGCGGGTCTAGACTGATAAAAGGCAGGTTACTGGCCCCAGGGGAGGGGAATTACAATTTGACATGCTACTGCCTATGCGACTCTTGGATTGGTTGTGATAGAAAGACAAACTTGAAGATGAAGATTTTGAAGCGCACACGGGCTGGTACCCGCGGAGGTTTTGTGGCGGAAGAGGGACCAATTACGGAGGATGGTATTGAAGAGGAGGAAGAGAATGAAGATGAGGAATATGATGACGATTGTGAGAGCGAGTACAGCGAAGATGAGGCGGATGAACAAATCGACACAAAAAAGAAAGGCCCTGCTACAAACGGCACTGTCGATAAACAAGGCTCCGGCTCCGATGGCTCAGGTTCGGACGACGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001655 GO:0001889 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006620 GO:0006807 GO:0006810 GO:0006886 GO:0007275 GO:0007568 GO:0008104 GO:0008150 GO:0008152 GO:0010259 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0034613 GO:0042175 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046907 GO:0048513 GO:0048731 GO:0048732 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061008 GO:0070727 GO:0070972 GO:0071702 GO:0071705 GO:0071944 GO:0072001 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko03060 ko04141 map03060 map04141

Pfam Domains

Protein Families

DnaJ Sec63

Protein Analysis

687

Amino Acids

76.61

Weight (kDa)

5.4

Isoelectric Point (pI)

47.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	100 - 160	1.2e-19	DnaJ domain
Sec63	PF02889	222 - 602	1.3e-19	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79940 AT1G79940 AT1G79940 AT1G79940
fragaria_vesca	FvH4_7g30440 FvH4_7g30440
malus_domestica	MD01G1210000.v1.1 MD07G1280100.v1.1
prunus_persica	Prupe.2G302300_v2.0.a1
pyrus_communis	pycom01g22050
rosa_chinensis	RchiOBHm_Chr1g0378591
rosa_laevigata	RLG00000026420
rosa_multiflora	Rmu_sc0002395.1_g000009
rosa_roxburghii	Rroxscaffold_4G00280370
rosa_rugosa	Rorug01G0409800
rosa_samantha	Rh1AG431300 Rh1BG389600 Rh1DG420800
rosa_wichuraiana	Rw1G037780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1205
Acc36I	ACCTGC	1 cut(s)	1707
Acc65I	GGTACC	1 cut(s)	1838
AccB1I	GGYRCC	2 cut(s)	1463, 1838
AccBSI	CCGCTC	1 cut(s)	1695
AccI	GTMKAC	1 cut(s)	1396
AccII	CGCG	2 cut(s)	279, 1845
AclI	AACGTT	1 cut(s)	1545
AclWI	GGATC	5 cut(s)	392, 452, 465, 1143, 1156
AcoI	YGGCCR	1 cut(s)	490
AcsI	RAATTY	3 cut(s)	475, 771, 1567
AcuI	CTGAAG	4 cut(s)	788, 1232, 1332, 1401
AfaI	GTAC	5 cut(s)	164, 377, 1281, 1840, 1946
AfiI	CCNNNNNNNGG	4 cut(s)	1057, 1079, 1735, 1736
AflII	CTTAAG	1 cut(s)	848
AflIII	ACRYGT	2 cut(s)	982, 1254
AjiI	CACGTC	1 cut(s)	983
AjnI	CCWGG	5 cut(s)	249, 322, 1050, 1317, 1729
AluBI	AGCT	9 cut(s)	329, 530, 626, 811, 1180, 1228, 1246, 1295, 1583
AluI	AGCT	9 cut(s)	329, 530, 626, 811, 1180, 1228, 1246, 1295, 1583
Alw21I	GWGCWC	1 cut(s)	140
Alw26I	GTCTC	3 cut(s)	355, 1370, 1540
AlwI	GGATC	5 cut(s)	392, 452, 465, 1143, 1156
AlwNI	CAGNNNCTG	2 cut(s)	451, 544
AoxI	GGCC	5 cut(s)	405, 490, 1070, 1725, 1990
ApeKI	GCWGC	8 cut(s)	5, 101, 755, 1177, 1180, 1583, 1586, 1661
ApoI	RAATTY	3 cut(s)	475, 771, 1567
ArsI	GACNNNNNNTTYG	4 cut(s)	1333, 1365, 1662, 1694
AseI	ATTAAT	1 cut(s)	221
Asp700I	GAANNNNTTC	1 cut(s)	801
Asp718I	GGTACC	1 cut(s)	1838
AspLEI	GCGC	2 cut(s)	149, 1827
AspS9I	GGNCC	5 cut(s)	406, 844, 1726, 1868, 1991
AsuHPI	GGTGA	4 cut(s)	631, 983, 1418, 1699
AvaII	GGWCC	2 cut(s)	844, 1868
BaeGI	GKGCMC	1 cut(s)	1468
BalI	TGGCCA	1 cut(s)	492
BamHI	GGATCC	2 cut(s)	457, 1148
BanI	GGYRCC	2 cut(s)	1463, 1838
BauI	CACGAG	2 cut(s)	468, 936
BbsI	GAAGAC	1 cut(s)	736
Bbv12I	GWGCWC	1 cut(s)	140
BbvCI	CCTCAGC	2 cut(s)	1067, 1275
BbvI	GCAGC	7 cut(s)	88, 742, 1167, 1189, 1570, 1598, 1648
BceAI	ACGGC	1 cut(s)	2020
BciT130I	CCWGG	5 cut(s)	251, 324, 1052, 1319, 1731
BcoDI	GTCTC	3 cut(s)	355, 1370, 1540
BfaI	CTAG	4 cut(s)	68, 567, 821, 1701
BfmI	CTRYAG	2 cut(s)	1175, 1584
BfrI	CTTAAG	1 cut(s)	848
BfuAI	ACCTGC	1 cut(s)	1707
BisI	GCNGC	9 cut(s)	6, 102, 277, 756, 1178, 1181, 1584, 1587, 1662
BlsI	GCNGC	9 cut(s)	7, 103, 278, 757, 1179, 1182, 1585, 1588, 1663
Bme1390I	CCNGG	5 cut(s)	251, 324, 1052, 1319, 1731
Bme18I	GGWCC	2 cut(s)	844, 1868
BmgBI	CACGTC	1 cut(s)	983
BmgT120I	GGNCC	5 cut(s)	406, 844, 1726, 1868, 1991
BmrFI	CCNGG	5 cut(s)	251, 324, 1052, 1319, 1731
BmsI	GCATC	7 cut(s)	113, 132, 572, 685, 898, 956, 1335
BpiI	GAAGAC	1 cut(s)	736
BpmI	CTGGAG	1 cut(s)	345
Bpu10I	CCTNAGC	3 cut(s)	1067, 1275, 2041
Bsa29I	ATCGAT	1 cut(s)	555
BsaBI	GATNNNNATC	2 cut(s)	390, 1303
BsaJI	CCNNGG	7 cut(s)	1050, 1051, 1260, 1605, 1729, 1730, 1843
BsaWI	WCCGGW	1 cut(s)	775
BsaXI	ACNNNNNCTCC	4 cut(s)	1520, 1550, 1839, 1869
Bsc4I	CCNNNNNNNGG	4 cut(s)	1057, 1079, 1735, 1736
Bse118I	RCCGGY	1 cut(s)	659
Bse1I	ACTGG	4 cut(s)	321, 461, 1152, 1728
Bse3DI	GCAATG	2 cut(s)	1051, 1161
Bse8I	GATNNNNATC	2 cut(s)	390, 1303
BseBI	CCWGG	5 cut(s)	251, 324, 1052, 1319, 1731
BseCI	ATCGAT	1 cut(s)	555
BseDI	CCNNGG	7 cut(s)	1050, 1051, 1260, 1605, 1729, 1730, 1843
BseJI	GATNNNNATC	2 cut(s)	390, 1303
BseLI	CCNNNNNNNGG	4 cut(s)	1057, 1079, 1735, 1736
BseMI	GCAATG	2 cut(s)	1051, 1161
BseMII	CTCAG	7 cut(s)	132, 551, 1058, 1112, 1220, 1266, 2055
BseNI	ACTGG	4 cut(s)	321, 461, 1152, 1728
BseRI	GAGGAG	1 cut(s)	1910
BseSI	GKGCMC	1 cut(s)	1468
BseXI	GCAGC	7 cut(s)	88, 742, 1167, 1189, 1570, 1598, 1648
BseYI	CCCAGC	1 cut(s)	725
Bsh1236I	CGCG	2 cut(s)	279, 1845
BshFI	GGCC	5 cut(s)	407, 492, 1072, 1727, 1992
BshNI	GGYRCC	2 cut(s)	1463, 1838
BshVI	ATCGAT	1 cut(s)	555
BsiHKAI	GWGCWC	1 cut(s)	140
BsiSI	CCGG	3 cut(s)	660, 776, 2028
BslFI	GGGAC	1 cut(s)	1881
BslI	CCNNNNNNNGG	4 cut(s)	1057, 1079, 1735, 1736
BsmAI	GTCTC	3 cut(s)	355, 1370, 1540
BsmFI	GGGAC	1 cut(s)	1881
BsnI	GGCC	5 cut(s)	407, 492, 1072, 1727, 1992
Bsp1286I	GDGCHC	2 cut(s)	140, 1468
Bsp143I	GATC	5 cut(s)	397, 457, 644, 1148, 1267
BspANI	GGCC	5 cut(s)	407, 492, 1072, 1727, 1992
BspCNI	CTCAG	7 cut(s)	133, 550, 1059, 1111, 1221, 1267, 2054
BspDI	ATCGAT	1 cut(s)	555
BspFNI	CGCG	2 cut(s)	279, 1845
BspHI	TCATGA	1 cut(s)	240
BspMAI	CTGCAG	2 cut(s)	1179, 1588
BspMI	ACCTGC	1 cut(s)	1707
BspPI	GGATC	5 cut(s)	392, 452, 465, 1143, 1156
BspQI	GCTCTTC	2 cut(s)	537, 1685
BspT107I	GGYRCC	2 cut(s)	1463, 1838
BspTI	CTTAAG	1 cut(s)	848
BsrBI	CCGCTC	1 cut(s)	1695
BsrDI	GCAATG	2 cut(s)	1051, 1161
BsrFI	RCCGGY	1 cut(s)	659
BsrI	ACTGG	4 cut(s)	321, 461, 1152, 1728
BssAI	RCCGGY	1 cut(s)	659
BssECI	CCNNGG	7 cut(s)	1050, 1051, 1260, 1605, 1729, 1730, 1843
BssMI	GATC	5 cut(s)	397, 457, 644, 1148, 1267
BssNAI	GTATAC	1 cut(s)	1397
BssSI	CACGAG	2 cut(s)	468, 936
BssT1I	CCWWGG	1 cut(s)	1605
Bst1107I	GTATAC	1 cut(s)	1397
Bst2BI	CACGAG	2 cut(s)	468, 936
Bst2UI	CCWGG	5 cut(s)	251, 324, 1052, 1319, 1731
Bst4CI	ACNGT	7 cut(s)	131, 375, 930, 1420, 1532, 1597, 2011
Bst6I	CTCTTC	6 cut(s)	9, 537, 1685, 1857, 1887, 1896
BstAFI	CTTAAG	1 cut(s)	848
BstAPI	GCANNNNNTGC	1 cut(s)	1345
BstC8I	GCNNGC	6 cut(s)	506, 870, 1074, 1219, 1446, 1524
BstDEI	CTNAG	8 cut(s)	141, 537, 1067, 1098, 1124, 1229, 1275, 2041
BstDSI	CCRYGG	2 cut(s)	1260, 1843
BstFNI	CGCG	2 cut(s)	279, 1845
BstHHI	GCGC	2 cut(s)	149, 1827
BstKTI	GATC	5 cut(s)	400, 460, 647, 1151, 1270
BstMAI	GTCTC	3 cut(s)	355, 1370, 1540
BstMBI	GATC	5 cut(s)	397, 457, 644, 1148, 1267
BstNI	CCWGG	5 cut(s)	251, 324, 1052, 1319, 1731
BstNSI	RCATGY	2 cut(s)	1258, 1758
BstSCI	CCNGG	5 cut(s)	249, 322, 1050, 1317, 1729
BstSFI	CTRYAG	2 cut(s)	1175, 1584
BstSLI	GKGCMC	1 cut(s)	1468
BstUI	CGCG	2 cut(s)	279, 1845
BstV1I	GCAGC	7 cut(s)	88, 742, 1167, 1189, 1570, 1598, 1648
BstV2I	GAAGAC	1 cut(s)	736
BstX2I	RGATCY	3 cut(s)	397, 457, 1148
BstXI	CCANNNNNNTGG	1 cut(s)	500
BstYI	RGATCY	3 cut(s)	397, 457, 1148
BstZ17I	GTATAC	1 cut(s)	1397
Bsu15I	ATCGAT	1 cut(s)	555
BsuRI	GGCC	5 cut(s)	407, 492, 1072, 1727, 1992
BsuTUI	ATCGAT	1 cut(s)	555
BtgI	CCRYGG	2 cut(s)	1260, 1843
BtrI	CACGTC	1 cut(s)	983
BtsI	GCAGTG	1 cut(s)	59
BtsIMutI	CAGTG	7 cut(s)	59, 127, 140, 1106, 1159, 1537, 2007
BveI	ACCTGC	1 cut(s)	1707
Cac8I	GCNNGC	6 cut(s)	506, 870, 1074, 1219, 1446, 1524
CaiI	CAGNNNCTG	2 cut(s)	451, 544
CciI	TCATGA	1 cut(s)	240
CfoI	GCGC	2 cut(s)	149, 1827
Cfr10I	RCCGGY	1 cut(s)	659
Cfr13I	GGNCC	5 cut(s)	406, 844, 1726, 1868, 1991
Cfr42I	CCGCGG	1 cut(s)	1846
ClaI	ATCGAT	1 cut(s)	555
Csp6I	GTAC	5 cut(s)	163, 376, 1280, 1839, 1945
CviAII	CATG	8 cut(s)	204, 241, 673, 706, 1255, 1424, 1573, 1755
CviQI	GTAC	5 cut(s)	163, 376, 1280, 1839, 1945
DdeI	CTNAG	8 cut(s)	141, 537, 1067, 1098, 1124, 1229, 1275, 2041
DpnI	GATC	5 cut(s)	399, 459, 646, 1150, 1269
DpnII	GATC	5 cut(s)	397, 457, 644, 1148, 1267
EaeI	YGGCCR	1 cut(s)	490
Eam1104I	CTCTTC	6 cut(s)	9, 537, 1685, 1857, 1887, 1896
EarI	CTCTTC	6 cut(s)	9, 537, 1685, 1857, 1887, 1896
EciI	GGCGGA	2 cut(s)	1874, 1976
Eco130I	CCWWGG	1 cut(s)	1605
Eco147I	AGGCCT	1 cut(s)	1072
Eco32I	GATATC	1 cut(s)	1356
Eco47I	GGWCC	2 cut(s)	844, 1868
Eco57I	CTGAAG	4 cut(s)	788, 1232, 1332, 1401
EcoO109I	RGGNCCY	1 cut(s)	1991
EcoRII	CCWGG	5 cut(s)	249, 322, 1050, 1317, 1729
EcoRV	GATATC	1 cut(s)	1356
EcoT14I	CCWWGG	1 cut(s)	1605
EcoT22I	ATGCAT	1 cut(s)	678
ErhI	CCWWGG	1 cut(s)	1605
FaeI	CATG	8 cut(s)	207, 244, 676, 709, 1258, 1427, 1576, 1758
FalI	AAGNNNNNCTT	2 cut(s)	1787, 1819
FaqI	GGGAC	1 cut(s)	1881
FatI	CATG	8 cut(s)	203, 240, 672, 705, 1254, 1423, 1572, 1754
FauI	CCCGC	3 cut(s)	1226, 1688, 1850
FblI	GTMKAC	1 cut(s)	1396
Fnu4HI	GCNGC	9 cut(s)	6, 102, 277, 756, 1178, 1181, 1584, 1587, 1662
Fsp4HI	GCNGC	9 cut(s)	6, 102, 277, 756, 1178, 1181, 1584, 1587, 1662
FspBI	CTAG	4 cut(s)	68, 567, 821, 1701
GlaI	GCGC	2 cut(s)	148, 1826
GluI	GCNGC	9 cut(s)	6, 102, 277, 756, 1178, 1181, 1584, 1587, 1662
GsaI	CCCAGC	1 cut(s)	729
GsuI	CTGGAG	1 cut(s)	345
HaeIII	GGCC	5 cut(s)	407, 492, 1072, 1727, 1992
HapII	CCGG	3 cut(s)	660, 776, 2028
HhaI	GCGC	2 cut(s)	149, 1827
Hin1II	CATG	8 cut(s)	207, 244, 676, 709, 1258, 1427, 1576, 1758
Hin6I	GCGC	2 cut(s)	147, 1825
HinP1I	GCGC	2 cut(s)	147, 1825
HincII	GTYRAC	1 cut(s)	933
HindII	GTYRAC	1 cut(s)	933
HindIII	AAGCTT	1 cut(s)	809
HinfI	GANTC	8 cut(s)	283, 335, 363, 838, 919, 941, 1299, 1772
HpaI	GTTAAC	1 cut(s)	933
HpaII	CCGG	3 cut(s)	660, 776, 2028
HphI	GGTGA	4 cut(s)	631, 983, 1418, 1699
Hpy166II	GTNNAC	2 cut(s)	933, 1397
Hpy188I	TCNGA	8 cut(s)	13, 142, 334, 340, 453, 807, 2035, 2050
Hpy8I	GTNNAC	2 cut(s)	933, 1397
Hpy99I	CGWCG	1 cut(s)	2057
HpyCH4III	ACNGT	7 cut(s)	131, 375, 930, 1420, 1532, 1597, 2011
HpyCH4IV	ACGT	2 cut(s)	982, 1545
HpyCH4V	TGCA	9 cut(s)	104, 676, 705, 967, 1130, 1177, 1339, 1526, 1586
HpyF3I	CTNAG	8 cut(s)	141, 537, 1067, 1098, 1124, 1229, 1275, 2041
HpySE526I	ACGT	2 cut(s)	982, 1545
Hsp92II	CATG	8 cut(s)	207, 244, 676, 709, 1258, 1427, 1576, 1758
HspAI	GCGC	2 cut(s)	147, 1825
KpnI	GGTACC	1 cut(s)	1842
KspAI	GTTAAC	1 cut(s)	933
KspI	CCGCGG	1 cut(s)	1846
Kzo9I	GATC	5 cut(s)	397, 457, 644, 1148, 1267
LguI	GCTCTTC	2 cut(s)	537, 1685
LmnI	GCTCC	6 cut(s)	326, 727, 1243, 1637, 2030, 2036
Lsp1109I	GCAGC	7 cut(s)	88, 742, 1167, 1189, 1570, 1598, 1648
LweI	GCATC	7 cut(s)	113, 132, 572, 685, 898, 956, 1335
MaeI	CTAG	4 cut(s)	68, 567, 821, 1701
MaeII	ACGT	2 cut(s)	982, 1545
MaeIII	GTNAC	5 cut(s)	209, 1406, 1414, 1432, 1719
MalI	GATC	5 cut(s)	399, 459, 646, 1150, 1269
MbiI	CCGCTC	1 cut(s)	1695
MboI	GATC	5 cut(s)	397, 457, 644, 1148, 1267
MfeI	CAATTG	2 cut(s)	1140, 1359
MflI	RGATCY	3 cut(s)	397, 457, 1148
MhlI	GDGCHC	2 cut(s)	140, 1468
MlsI	TGGCCA	1 cut(s)	492
MluNI	TGGCCA	1 cut(s)	492
MlyI	GAGTC	3 cut(s)	357, 950, 1766
Mox20I	TGGCCA	1 cut(s)	492
Mph1103I	ATGCAT	1 cut(s)	678
MroXI	GAANNNNTTC	1 cut(s)	801
MscI	TGGCCA	1 cut(s)	492
MseI	TTAA	4 cut(s)	180, 221, 849, 932
MslI	CAYNNNNRTG	4 cut(s)	498, 555, 1191, 1259
Msp20I	TGGCCA	1 cut(s)	492
MspA1I	CMGCKG	4 cut(s)	1180, 1295, 1310, 1845
MspCI	CTTAAG	1 cut(s)	848
MspI	CCGG	3 cut(s)	660, 776, 2028
MspR9I	CCNGG	5 cut(s)	251, 324, 1052, 1319, 1731
MunI	CAATTG	2 cut(s)	1140, 1359
MvaI	CCWGG	5 cut(s)	251, 324, 1052, 1319, 1731
MvnI	CGCG	2 cut(s)	279, 1845
NdeII	GATC	5 cut(s)	397, 457, 644, 1148, 1267
NlaIII	CATG	8 cut(s)	207, 244, 676, 709, 1258, 1427, 1576, 1758
NmuCI	GTSAC	3 cut(s)	1406, 1414, 1432
NsiI	ATGCAT	1 cut(s)	678
NspI	RCATGY	2 cut(s)	1258, 1758
PagI	TCATGA	1 cut(s)	240
PasI	CCCWGGG	2 cut(s)	1051, 1730
PceI	AGGCCT	1 cut(s)	1072
PciI	ACATGT	1 cut(s)	1254
PciSI	GCTCTTC	2 cut(s)	537, 1685
PcsI	WCGNNNNNNNCGW	2 cut(s)	1349, 2010
PdmI	GAANNNNTTC	1 cut(s)	801
PfeI	GAWTC	5 cut(s)	283, 335, 838, 919, 1299
PflFI	GACNNNGTC	2 cut(s)	1412, 1532
PfoI	TCCNGGA	1 cut(s)	1317
PkrI	GCNGC	9 cut(s)	7, 103, 278, 757, 1179, 1182, 1585, 1588, 1663
PleI	GAGTC	3 cut(s)	357, 949, 1766
PpsI	GAGTC	3 cut(s)	357, 949, 1766
PscI	ACATGT	1 cut(s)	1254
PshBI	ATTAAT	1 cut(s)	221
PsiI	TTATAA	1 cut(s)	1205
Psp1406I	AACGTT	1 cut(s)	1545
Psp6I	CCWGG	5 cut(s)	249, 322, 1050, 1317, 1729
PspFI	CCCAGC	1 cut(s)	725
PspGI	CCWGG	5 cut(s)	249, 322, 1050, 1317, 1729
PspPI	GGNCC	5 cut(s)	406, 844, 1726, 1868, 1991
PstI	CTGCAG	2 cut(s)	1179, 1588
PstNI	CAGNNNCTG	2 cut(s)	451, 544
PsuI	RGATCY	3 cut(s)	397, 457, 1148
PsyI	GACNNNGTC	2 cut(s)	1412, 1532
PvuII	CAGCTG	2 cut(s)	1180, 1295
RsaI	GTAC	5 cut(s)	164, 377, 1281, 1840, 1946
RsaNI	GTAC	5 cut(s)	163, 376, 1280, 1839, 1945
RseI	CAYNNNNRTG	4 cut(s)	498, 555, 1191, 1259
SacII	CCGCGG	1 cut(s)	1846
SapI	GCTCTTC	2 cut(s)	537, 1685
SaqAI	TTAA	4 cut(s)	180, 221, 849, 932
SatI	GCNGC	9 cut(s)	6, 102, 277, 756, 1178, 1181, 1584, 1587, 1662
Sau3AI	GATC	5 cut(s)	397, 457, 644, 1148, 1267
Sau96I	GGNCC	5 cut(s)	406, 844, 1726, 1868, 1991
SchI	GAGTC	3 cut(s)	357, 950, 1766
ScrFI	CCNGG	5 cut(s)	251, 324, 1052, 1319, 1731
SduI	GDGCHC	2 cut(s)	140, 1468
SfaNI	GCATC	7 cut(s)	113, 132, 572, 685, 898, 956, 1335
SfcI	CTRYAG	2 cut(s)	1175, 1584
Sfr303I	CCGCGG	1 cut(s)	1846
SgrBI	CCGCGG	1 cut(s)	1846
SinI	GGWCC	2 cut(s)	844, 1868
SmiMI	CAYNNNNRTG	4 cut(s)	498, 555, 1191, 1259
SmlI	CTYRAG	1 cut(s)	848
SmoI	CTYRAG	1 cut(s)	848
SseBI	AGGCCT	1 cut(s)	1072
SspMI	CTAG	4 cut(s)	68, 567, 821, 1701
StuI	AGGCCT	1 cut(s)	1072
StyD4I	CCNGG	5 cut(s)	249, 322, 1050, 1317, 1729
StyI	CCWWGG	1 cut(s)	1605
TaaI	ACNGT	7 cut(s)	131, 375, 930, 1420, 1532, 1597, 2011
TaiI	ACGT	2 cut(s)	985, 1548
TaqI	TCGA	7 cut(s)	294, 555, 1352, 1614, 1680, 1974, 2013
TaqII	GACCGA	1 cut(s)	141
TatI	WGTACW	1 cut(s)	1944
TauI	GCSGC	1 cut(s)	279
TfiI	GAWTC	5 cut(s)	283, 335, 838, 919, 1299
Tru1I	TTAA	4 cut(s)	180, 221, 849, 932
Tru9I	TTAA	4 cut(s)	180, 221, 849, 932
TscAI	CASTG	7 cut(s)	66, 134, 140, 1106, 1159, 1537, 2014
TseFI	GTSAC	3 cut(s)	1406, 1414, 1432
TseI	GCWGC	8 cut(s)	5, 101, 755, 1177, 1180, 1583, 1586, 1661
Tsp45I	GTSAC	3 cut(s)	1406, 1414, 1432
TspGWI	ACGGA	3 cut(s)	1249, 1467, 1892
TspRI	CASTG	7 cut(s)	66, 134, 140, 1106, 1159, 1537, 2014
Tth111I	GACNNNGTC	2 cut(s)	1412, 1532
Vha464I	CTTAAG	1 cut(s)	848
VpaK11BI	GGWCC	2 cut(s)	844, 1868
VspI	ATTAAT	1 cut(s)	221
XapI	RAATTY	3 cut(s)	475, 771, 1567
XbaI	TCTAGA	1 cut(s)	1700
XceI	RCATGY	2 cut(s)	1258, 1758
XcmI	CCANNNNNNNNNTGG	1 cut(s)	733
XmiI	GTMKAC	1 cut(s)	1396
XmnI	GAANNNNTTC	1 cut(s)	801
XspI	CTAG	4 cut(s)	68, 567, 821, 1701
Zsp2I	ATGCAT	1 cut(s)	678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.