MD01G1214100.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009719
Physical Location & Seq
Forward (+)
30754649 .. 30757652
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1214100.v1.1.491

Sequence Viewer

Length: 1875 bp
ATGGAGAAAAGAGTAGAGCTTTTGGTCGTTTTGGTTACTGGGGCAATTTTCTTGCATGTCACTGCCGACCCAGTTGATGATAAGCAAGCCTTGTTTGATTTCATTCACAACATTTCTCACTCAACCTCTATCAAATGGAATGAGAGTTCTTCTGTTTGCAAAACCTGGACCGGCGTGGTCTGTAGCAAAGATCAGTCCGGAGTTATAGAGCTTCATTTGCCTGGAGCTGCCTTACATGGTCCAATCCCACCGAACACTCTCAGCCGGCTTTCGGCGCTTCAGGTTCTAAATCTCAGTTCGAATACCTTGACAGGTCCATTCCCTTCTGATTTTTCCAAACTCAGAAACTTGTCTTCCCTTTATCTTCAGTACAACAATTTCTCCGGCCCATTGCCTTTAGATTTTTCGGTTTGGAAGAATGTTACTGTCATGAATCTCTCAAACAATGCATTTAGTGGGAATATCTCTTCCTCGATTTCAAGTTTGACTCACCTCACAGTTCTGAATCTCGCTAACAATTCGCTTTCGGGTGAAATTCCTGACCTCAATGTTCCTAGTTTGCAGCAGCTTGATTTGGCTAACAATAACCTCTCAGGGAATGTGCCTCAATCTCTTCAAAGATTTCCAAGTTGGGCCTTTTCTGGTAACAGTCTTGCGTCCCTTCCTCCAGCTCTTCCGGTTCAACCACCTAATTCTAAACCGAGGAAGAAAGTGAAACTCGGCAAGCATGCAATGCTAGGTATTGTGATTGGTGGATGTGTTCTGGGATTTGTTTTAATTGCTGTTCTGATGATCATTTGCCGTTACAACAAAGAAGGCGAAAATGGGATAGTTGAAAAACCAAAGAAAGAACTCTTTTCGAAGGAAGGGCTTTCTCGGAAGCATGATAAGAATAACAGGATTTCGTTCTTCGAGGGTTCTAATCTTGCATTTGATCTGGAGGACTTGTTGAGGGCGTCTGCTGACGTTCTTGGCAAGGGAACATTCGGGACGACATATAAGGCAGCTTTAGAAGATGCAACTACTGTTGTGGTGAAGAGGTTGAAGGAAGTGAGTGTGGGAAAAAAGGAATTTGAGCAGCAAATAGAGATTGTGGGAAGTATCAGGCATGAGAATATAGCCTCGCTGAGGGCGTATTACTATTCAAAAGATGAGAAGCTTGTTGTTTATGATTACTATGAGCAGGGAAGCGCCTCGTCCCTGTTACATGCTAAAAGAGGAGAGGGCAGGATTCCATTCGACTGGGAGACTCGACTGAAAATAGCAATCGGTGCAGCAAGAGGCATCGCTCATATCCACACGCAGAATGGTGGGAAACTTGTCCATGGAAACATAAAGGCCTCAAACATCTTCCTCAACTCTCAAGGATACGGTTGTGTGTGCGATGTTGGTTTGCCAACACTGATGGGTCCAACGCCCCCACCAGCAGCGCGTACTGGAGGATACCGAGCCCCAGAAGTGAAAGACACCCGTAAGTCTTCCCCTGCATCTGATGTCTACAGTTTCGGGGTCCTGCTACTTGAGCTTCTCACCGGAAAAAGCCCCATACACACAATAGGTGGCGAAGAAGTTGTTCACTTGGTCCGGTGGGTGAACTCTGTGGTCAGAGAGGAATGGACAGCAGAAGTGTTTGACGTAGAGCTTCTGCGGTATCCAAACATAGAGGAGGAAATGGTAGAGATGTTACAAATAGGAATGTCTTGTGTGGCAAGAATGCCTGAGCAACGACCAAATATGCCGGATTTGGTGAAAAGGGTGGAAGAAATCCGTCAAGTAAACACCGGAACTCGACCATCATCAGGATTATCTACTCCAATCTTAAATACACCGACTCCTCCATCGGAGATAGCAGGACCTTCCTCTCTACCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

625

Amino Acids

68.16

Weight (kDa)

8.04

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 62 7.7e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 82 - 127 9.1e-09 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 322 - 585 1.7e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 322 - 584 4.2e-31 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1497
AccII CGCG 1 cut(s) 1432
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 1 cut(s) 1648
AcsI RAATTY 2 cut(s) 534, 1070
AcuI CTGAAG 2 cut(s) 263, 350
AcyI GRCGYC 1 cut(s) 956
AfaI GTAC 2 cut(s) 371, 1435
AfiI CCNNNNNNNGG 3 cut(s) 271, 1128, 1799
AgsI TTSAA 6 cut(s) 480, 617, 683, 836, 1045, 1146
AjnI CCWGG 2 cut(s) 164, 220
AloI GAACNNNNNNTCC 2 cut(s) 130, 162
AluBI AGCT 9 cut(s) 19, 211, 227, 568, 671, 1007, 1159, 1525, 1642
AluI AGCT 9 cut(s) 19, 211, 227, 568, 671, 1007, 1159, 1525, 1642
Alw26I GTCTC 1 cut(s) 1241
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 3 cut(s) 385, 633, 1338
ApeKI GCWGC 7 cut(s) 227, 562, 565, 1004, 1078, 1274, 1427
ApoI RAATTY 2 cut(s) 534, 1070
Asp700I GAANNNNTTC 1 cut(s) 1572
AspLEI GCGC 3 cut(s) 277, 1193, 1432
AspS9I GGNCC 9 cut(s) 168, 239, 314, 386, 633, 1409, 1510, 1582, 1853
AsuHPI GGTGA 6 cut(s) 482, 542, 1045, 1522, 1603, 1759
AsuII TTCGAA 2 cut(s) 299, 860
AvaII GGWCC 7 cut(s) 168, 239, 314, 1409, 1510, 1582, 1853
BaeI ACNNNNGTAYC 2 cut(s) 1360, 1393
BanII GRGCYC 1 cut(s) 1453
BbsI GAAGAC 2 cut(s) 345, 1470
BbvCI CCTCAGC 1 cut(s) 1127
BbvI GCAGC 7 cut(s) 214, 574, 577, 1016, 1090, 1286, 1439
BccI CCATC 3 cut(s) 1399, 1801, 1846
BceAI ACGGC 1 cut(s) 786
BciT130I CCWGG 2 cut(s) 166, 222
BciVI GTATCC 3 cut(s) 1361, 1436, 1662
BclI TGATCA 1 cut(s) 792
BcoDI GTCTC 1 cut(s) 1241
BfaI CTAG 2 cut(s) 555, 737
BfmI CTRYAG 2 cut(s) 181, 1498
BfoI RGCGCY 2 cut(s) 278, 1194
BfuI GTATCC 3 cut(s) 1361, 1436, 1662
BisI GCNGC 7 cut(s) 228, 563, 566, 1005, 1079, 1275, 1428
BlsI GCNGC 7 cut(s) 229, 564, 567, 1006, 1080, 1276, 1429
Bme1390I CCNGG 2 cut(s) 166, 222
Bme18I GGWCC 7 cut(s) 168, 239, 314, 1409, 1510, 1582, 1853
BmgT120I GGNCC 9 cut(s) 168, 239, 314, 386, 633, 1409, 1510, 1582, 1853
BmiI GGNNCC 2 cut(s) 1410, 1511
BmrFI CCNGG 2 cut(s) 166, 222
BmrI ACTGGG 4 cut(s) 48, 65, 1252, 1864
BmsI GCATC 3 cut(s) 1006, 1293, 1496
BmuI ACTGGG 4 cut(s) 48, 65, 1252, 1864
BpiI GAAGAC 2 cut(s) 345, 1470
BpmI CTGGAG 4 cut(s) 243, 651, 959, 1458
Bpu10I CCTNAGC 2 cut(s) 1127, 1719
Bpu14I TTCGAA 2 cut(s) 299, 860
BpuEI CTTGAG 2 cut(s) 1347, 1541
BsaHI GRCGYC 1 cut(s) 956
BsaJI CCNNGG 2 cut(s) 701, 1324
BsaWI WCCGGW 5 cut(s) 197, 676, 1532, 1584, 1781
BsaXI ACNNNNNCTCC 6 cut(s) 365, 395, 932, 962, 1816, 1846
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 1128, 1799
Bse118I RCCGGY 2 cut(s) 170, 264
Bse1I ACTGG 5 cut(s) 43, 71, 1247, 1441, 1870
Bse3DI GCAATG 2 cut(s) 389, 738
BseAI TCCGGA 1 cut(s) 197
BseBI CCWGG 2 cut(s) 166, 222
BseDI CCNNGG 2 cut(s) 701, 1324
BseGI GGATG 1 cut(s) 761
BseLI CCNNNNNNNGG 3 cut(s) 271, 1128, 1799
BseMI GCAATG 2 cut(s) 389, 738
BseMII CTCAG 6 cut(s) 274, 307, 355, 606, 1118, 1710
BseNI ACTGG 5 cut(s) 43, 71, 1247, 1441, 1870
BseRI GAGGAG 3 cut(s) 1233, 1679, 1824
BseXI GCAGC 7 cut(s) 214, 574, 577, 1016, 1090, 1286, 1439
BsgI GTGCAG 1 cut(s) 1293
Bsh1236I CGCG 1 cut(s) 1432
BshFI GGCC 3 cut(s) 387, 635, 1340
BsiSI CCGG 9 cut(s) 171, 198, 265, 384, 677, 1533, 1585, 1739, 1782
BslFI GGGAC 3 cut(s) 643, 1003, 1183
BslI CCNNNNNNNGG 3 cut(s) 271, 1128, 1799
BsmAI GTCTC 1 cut(s) 1241
BsmFI GGGAC 3 cut(s) 643, 1003, 1183
BsmI GAATGC 1 cut(s) 1719
BsnI GGCC 3 cut(s) 387, 635, 1340
Bsp119I TTCGAA 2 cut(s) 299, 860
Bsp1286I GDGCHC 1 cut(s) 1453
Bsp13I TCCGGA 1 cut(s) 197
Bsp143I GATC 3 cut(s) 190, 792, 934
Bsp19I CCATGG 1 cut(s) 1324
BspACI CCGC 1 cut(s) 1648
BspANI GGCC 3 cut(s) 387, 635, 1340
BspCNI CTCAG 6 cut(s) 273, 306, 354, 605, 1119, 1711
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 1 cut(s) 1432
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 2 cut(s) 1410, 1511
BspQI GCTCTTC 1 cut(s) 678
BspT104I TTCGAA 2 cut(s) 299, 860
BsrDI GCAATG 2 cut(s) 389, 738
BsrFI RCCGGY 2 cut(s) 170, 264
BsrI ACTGG 5 cut(s) 43, 71, 1247, 1441, 1870
BssAI RCCGGY 2 cut(s) 170, 264
BssECI CCNNGG 2 cut(s) 701, 1324
BssMI GATC 3 cut(s) 190, 792, 934
BssNI GRCGYC 1 cut(s) 956
BssT1I CCWWGG 1 cut(s) 1324
Bst2UI CCWGG 2 cut(s) 166, 222
Bst4CI ACNGT 6 cut(s) 427, 499, 650, 1027, 1373, 1502
Bst6I CTCTTC 4 cut(s) 472, 618, 678, 1031
BstACI GRCGYC 1 cut(s) 956
BstAPI GCANNNNNTGC 2 cut(s) 733, 1271
BstBI TTCGAA 2 cut(s) 299, 860
BstC8I GCNNGC 4 cut(s) 87, 266, 725, 729
BstDEI CTNAG 6 cut(s) 260, 293, 341, 592, 1127, 1719
BstDSI CCRYGG 1 cut(s) 1324
BstENI CCTNNNNNAGG 1 cut(s) 1126
BstF5I GGATG 1 cut(s) 761
BstFNI CGCG 1 cut(s) 1432
BstH2I RGCGCY 2 cut(s) 278, 1194
BstHHI GCGC 3 cut(s) 277, 1193, 1432
BstKTI GATC 3 cut(s) 193, 795, 937
BstMAI GTCTC 1 cut(s) 1241
BstMBI GATC 3 cut(s) 190, 792, 934
BstMWI GCNNNNNNNGC 5 cut(s) 217, 274, 733, 1271, 1522
BstNI CCWGG 2 cut(s) 166, 222
BstNSI RCATGY 3 cut(s) 59, 731, 1211
BstSCI CCNGG 2 cut(s) 164, 220
BstSFI CTRYAG 2 cut(s) 181, 1498
BstUI CGCG 1 cut(s) 1432
BstV1I GCAGC 7 cut(s) 214, 574, 577, 1016, 1090, 1286, 1439
BstV2I GAAGAC 2 cut(s) 345, 1470
BstXI CCANNNNNNTGG 1 cut(s) 1242
BsuI GTATCC 3 cut(s) 1361, 1436, 1662
BsuRI GGCC 3 cut(s) 387, 635, 1340
BtgI CCRYGG 1 cut(s) 1324
BtgZI GCGATG 2 cut(s) 1270, 1398
BtsCI GGATG 1 cut(s) 761
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 60, 1400
Cac8I GCNNGC 4 cut(s) 87, 266, 725, 729
CciI TCATGA 1 cut(s) 429
CfoI GCGC 3 cut(s) 277, 1193, 1432
Cfr10I RCCGGY 2 cut(s) 170, 264
Cfr13I GGNCC 9 cut(s) 168, 239, 314, 386, 633, 1409, 1510, 1582, 1853
CseI GACGC 2 cut(s) 645, 945
Csp6I GTAC 2 cut(s) 370, 1434
CviAII CATG 8 cut(s) 56, 236, 430, 728, 884, 1109, 1208, 1325
CviQI GTAC 2 cut(s) 370, 1434
DdeI CTNAG 6 cut(s) 260, 293, 341, 592, 1127, 1719
DpnI GATC 3 cut(s) 192, 794, 936
DpnII GATC 3 cut(s) 190, 792, 934
Eam1104I CTCTTC 4 cut(s) 472, 618, 678, 1031
EarI CTCTTC 4 cut(s) 472, 618, 678, 1031
Eco130I CCWWGG 1 cut(s) 1324
Eco147I AGGCCT 1 cut(s) 1340
Eco24I GRGCYC 1 cut(s) 1453
Eco47I GGWCC 7 cut(s) 168, 239, 314, 1409, 1510, 1582, 1853
Eco57I CTGAAG 2 cut(s) 263, 350
EcoNI CCTNNNNNAGG 1 cut(s) 1126
EcoO109I RGGNCCY 2 cut(s) 1510, 1853
EcoRII CCWGG 2 cut(s) 164, 220
EcoT14I CCWWGG 1 cut(s) 1324
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 1453
ErhI CCWWGG 1 cut(s) 1324
FaeI CATG 8 cut(s) 59, 239, 433, 731, 887, 1112, 1211, 1328
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 3 cut(s) 643, 1003, 1183
FatI CATG 8 cut(s) 55, 235, 429, 727, 883, 1108, 1207, 1324
FbaI TGATCA 1 cut(s) 792
FblI GTMKAC 1 cut(s) 1497
Fnu4HI GCNGC 7 cut(s) 228, 563, 566, 1005, 1079, 1275, 1428
FokI GGATG 1 cut(s) 768
FriOI GRGCYC 1 cut(s) 1453
Fsp4HI GCNGC 7 cut(s) 228, 563, 566, 1005, 1079, 1275, 1428
FspBI CTAG 2 cut(s) 555, 737
GlaI GCGC 3 cut(s) 276, 1192, 1431
GluI GCNGC 7 cut(s) 228, 563, 566, 1005, 1079, 1275, 1428
GsuI CTGGAG 4 cut(s) 243, 651, 959, 1458
HaeII RGCGCY 2 cut(s) 278, 1194
HaeIII GGCC 3 cut(s) 387, 635, 1340
HapII CCGG 9 cut(s) 171, 198, 265, 384, 677, 1533, 1585, 1739, 1782
HgaI GACGC 2 cut(s) 645, 945
HhaI GCGC 3 cut(s) 277, 1193, 1432
Hin1I GRCGYC 1 cut(s) 956
Hin1II CATG 8 cut(s) 59, 239, 433, 731, 887, 1112, 1211, 1328
Hin6I GCGC 3 cut(s) 275, 1191, 1430
HinP1I GCGC 3 cut(s) 275, 1191, 1430
HindIII AAGCTT 1 cut(s) 1157
HinfI GANTC 6 cut(s) 433, 487, 505, 1231, 1249, 1831
HpaII CCGG 9 cut(s) 171, 198, 265, 384, 677, 1533, 1585, 1739, 1782
HphI GGTGA 6 cut(s) 482, 542, 1045, 1522, 1603, 1759
Hpy166II GTNNAC 4 cut(s) 1498, 1576, 1594, 1777
Hpy188I TCNGA 8 cut(s) 328, 344, 504, 789, 879, 1492, 1607, 1843
Hpy188III TCNNGA 6 cut(s) 198, 430, 539, 938, 988, 1800
Hpy8I GTNNAC 4 cut(s) 1498, 1576, 1594, 1777
HpyAV CCTTC 7 cut(s) 333, 671, 809, 856, 860, 1039, 1866
HpyCH4III ACNGT 6 cut(s) 427, 499, 650, 1027, 1373, 1502
HpyCH4IV ACGT 2 cut(s) 966, 1635
HpyCH4V TGCA 9 cut(s) 55, 159, 449, 562, 731, 929, 1019, 1274, 1487
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 274, 733, 1271, 1522
HpyF3I CTNAG 6 cut(s) 260, 293, 341, 592, 1127, 1719
HpySE526I ACGT 2 cut(s) 966, 1635
Hsp92I GRCGYC 1 cut(s) 956
Hsp92II CATG 8 cut(s) 59, 239, 433, 731, 887, 1112, 1211, 1328
HspAI GCGC 3 cut(s) 275, 1191, 1430
Kpn2I TCCGGA 1 cut(s) 197
KroI GCCGGC 1 cut(s) 264
KroNI GCCGGC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 792
Kzo9I GATC 3 cut(s) 190, 792, 934
LguI GCTCTTC 1 cut(s) 678
LmnI GCTCC 1 cut(s) 224
Lsp1109I GCAGC 7 cut(s) 214, 574, 577, 1016, 1090, 1286, 1439
LweI GCATC 3 cut(s) 1006, 1293, 1496
MaeI CTAG 2 cut(s) 555, 737
MaeII ACGT 2 cut(s) 966, 1635
MaeIII GTNAC 7 cut(s) 34, 58, 421, 644, 803, 1203, 1683
MalI GATC 3 cut(s) 192, 794, 936
MboI GATC 3 cut(s) 190, 792, 934
MhlI GDGCHC 1 cut(s) 1453
MluCI AATT 7 cut(s) 45, 376, 517, 534, 691, 777, 1070
MlyI GAGTC 3 cut(s) 481, 1243, 1825
MmeI TCCRAC 1 cut(s) 1436
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 1 cut(s) 197
MroNI GCCGGC 1 cut(s) 264
MroXI GAANNNNTTC 1 cut(s) 1572
MseI TTAA 2 cut(s) 776, 1820
MspI CCGG 9 cut(s) 171, 198, 265, 384, 677, 1533, 1585, 1739, 1782
MspR9I CCNGG 2 cut(s) 166, 222
Mva1269I GAATGC 1 cut(s) 1719
MvaI CCWGG 2 cut(s) 166, 222
MvnI CGCG 1 cut(s) 1432
MwoI GCNNNNNNNGC 5 cut(s) 217, 274, 733, 1271, 1522
NaeI GCCGGC 1 cut(s) 266
NcoI CCATGG 1 cut(s) 1324
NdeII GATC 3 cut(s) 190, 792, 934
NgoMIV GCCGGC 1 cut(s) 264
NlaIII CATG 8 cut(s) 59, 239, 433, 731, 887, 1112, 1211, 1328
NlaIV GGNNCC 2 cut(s) 1410, 1511
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 3 cut(s) 59, 731, 1211
NspV TTCGAA 2 cut(s) 299, 860
PaeI GCATGC 1 cut(s) 731
PagI TCATGA 1 cut(s) 429
PceI AGGCCT 1 cut(s) 1340
PciSI GCTCTTC 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 1130
PctI GAATGC 1 cut(s) 1719
PdiI GCCGGC 1 cut(s) 266
PdmI GAANNNNTTC 1 cut(s) 1572
PfeI GAWTC 3 cut(s) 433, 505, 1231
PkrI GCNGC 7 cut(s) 229, 564, 567, 1006, 1080, 1276, 1429
PleI GAGTC 3 cut(s) 481, 1243, 1825
PpsI GAGTC 3 cut(s) 481, 1243, 1825
PpuMI RGGWCCY 2 cut(s) 1510, 1853
Psp5II RGGWCCY 2 cut(s) 1510, 1853
Psp6I CCWGG 2 cut(s) 164, 220
PspGI CCWGG 2 cut(s) 164, 220
PspN4I GGNNCC 2 cut(s) 1410, 1511
PspPI GGNCC 9 cut(s) 168, 239, 314, 386, 633, 1409, 1510, 1582, 1853
PspPPI RGGWCCY 2 cut(s) 1510, 1853
RsaI GTAC 2 cut(s) 371, 1435
RsaNI GTAC 2 cut(s) 370, 1434
SapI GCTCTTC 1 cut(s) 678
SaqAI TTAA 2 cut(s) 776, 1820
SatI GCNGC 7 cut(s) 228, 563, 566, 1005, 1079, 1275, 1428
Sau3AI GATC 3 cut(s) 190, 792, 934
Sau96I GGNCC 9 cut(s) 168, 239, 314, 386, 633, 1409, 1510, 1582, 1853
SchI GAGTC 3 cut(s) 481, 1243, 1825
ScrFI CCNGG 2 cut(s) 166, 222
SduI GDGCHC 1 cut(s) 1453
SfaNI GCATC 3 cut(s) 1006, 1293, 1496
SfcI CTRYAG 2 cut(s) 181, 1498
SfuI TTCGAA 2 cut(s) 299, 860
SinI GGWCC 7 cut(s) 168, 239, 314, 1409, 1510, 1582, 1853
SmlI CTYRAG 2 cut(s) 1362, 1520
SmoI CTYRAG 2 cut(s) 1362, 1520
SphI GCATGC 1 cut(s) 731
Sse9I AATT 7 cut(s) 45, 376, 517, 534, 691, 777, 1070
SseBI AGGCCT 1 cut(s) 1340
SsiI CCGC 1 cut(s) 1648
SspMI CTAG 2 cut(s) 555, 737
StuI AGGCCT 1 cut(s) 1340
StyD4I CCNGG 2 cut(s) 164, 220
StyI CCWWGG 1 cut(s) 1324
TaaI ACNGT 6 cut(s) 427, 499, 650, 1027, 1373, 1502
TaiI ACGT 2 cut(s) 969, 1638
TaqI TCGA 7 cut(s) 299, 473, 860, 912, 1239, 1252, 1789
TasI AATT 7 cut(s) 45, 376, 517, 534, 691, 777, 1070
TatI WGTACW 1 cut(s) 369
TfiI GAWTC 3 cut(s) 433, 505, 1231
Tru1I TTAA 2 cut(s) 776, 1820
Tru9I TTAA 2 cut(s) 776, 1820
TscAI CASTG 2 cut(s) 67, 1407
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 7 cut(s) 227, 562, 565, 1004, 1078, 1274, 1427
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 3 cut(s) 91, 203, 446
TspGWI ACGGA 1 cut(s) 1757
TspRI CASTG 2 cut(s) 67, 1407
VpaK11BI GGWCC 7 cut(s) 168, 239, 314, 1409, 1510, 1582, 1853
XagI CCTNNNNNAGG 1 cut(s) 1126
XapI RAATTY 2 cut(s) 534, 1070
XceI RCATGY 3 cut(s) 59, 731, 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 1304
XmiI GTMKAC 1 cut(s) 1497
XmnI GAANNNNTTC 1 cut(s) 1572
XspI CTAG 2 cut(s) 555, 737
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.