MD07G1283700.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009719
Physical Location & Seq
Forward (+)
34649347 .. 34652300
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1283700.v1.1.491

Sequence Viewer

Length: 1890 bp
ATGGGGAAAAGATTAGAGCTTTTGGTCGTTTTGTTCATTGGGGTAATTTTCTTGCATGTCACTGCGGACCCAGTTGAAGATAAGCAAGCTTTGTTGGATTTCATTCACAATATTTCTCACTCGCGCTCTATCAAATGGAATGAGAATTCTTCTGTTTGCGAAACCTGGAACGGCGTGATCTGTAGCGAAGATCAGTCCAGAGTTATAGAGCTTCATTTGCCTGGAGCTGCCTTACTTGGTCCAATCCCACCGAACACTCTCAGCCGGCTTTCGGCGCTTCTGGTTCTAAGTCTAAGGTTGAATAGCTTGACAGGTCCATTCCCTTCTGATTTTAACAAACTCGAAAACTTGTCTTCCCTTTATCTTCAATTCAATAACTTCTCTGGCCCTTTGCCTTTAAATTTTTCGGTTTGGAAGAATCTTACTGTCATGAATCTGTCAAACAATGCATTTAGTGGGAATATCCCTTCCTCGATTTCAAATTTGACTCACCTCACAGTTCTGAATCTCGCTAACAATTCGCTTTCGGGTGAAATTCCTGACCTCAATGTTCCTAGTTTGGAACAGCTTGATTTGGCTAACAATAACCTCTCAGGGATTGTTCCTCAATCTCTTCAAAGATTTCCAAGTTGGGCCTTTTCAGGCAACAGTCTGGCATCAGCCCTTCCTCCAGCTCTTCCTGTTGAACCACCTAATTCTCAACCAAGAAAGAAAACGAGAATCAGTCAGCCTGCAATACTAGGTATTGTGATCGGCGGATGTGTTCTGGGATTCGTTCTAATTGCTTTTTTTATGCTCGTTTGCTGCCGCAATAAGGAAGGCGAAAATGGGAAAGTTGAAAAACCAAAGAAGAAAGAACTCTTTTCAAAGAAAGGGGTTTCTGAGAAGCAAAATAAGGATACCAAGCTTTCCTTCTTTGAGGGTTCTAATCTTGCATTTGATCTGGACGATTTGTTGAGGGCATCTGCTGAGGTTCTTGGCAAGGGAACGTTCGGGACAACATATAAGGCTGCTTTGGAAGATGCAACCAATGCAACCACTGTTGTGGTGAAGAGGTTGAAGGAAGTGAGTGTGGGAAAGAAGGAATTTGAGCAGCAAATGGAGATTGTGGGAAGTATCAAGCATGAGAATGTAGCCGCACTAAGGGCGTATTACTATTCGAAAGATGAGAAGCTTGTTGTGTATGATTACTATGAGCAGGGAAGCGCCTCTTCGATGTTACATGCTAAAAGAGGAGAAGGCAGGATTCCGCTTAACTGGGAGACTCGACTCAAAATATCAATTGGTGCAGCAAGAGGCATAGCTCATATCCACACTCAGAACGCTGGGAAACTTGTCCATGGAAACATAAAGGCCTCAAACATCTTCCTCAATTCTCAAGGATACGGTTGTGTGTGCGATGTTGGTTTGCCAACACTGATGAGTCCAATACCCCCACCAGCAGTGCGTACAGGAGGATACCGAGCCCCAGAAGTGACAGACACCCGCAAGTCTACCCCTGCATCAGATGTCTACAGCTTCGGGGTCCTGATACTCGAGCTTCTCACCGGTAAAAGCCCCATCCACACAACAGACGGGGAAGAAGTTATTCACTTGGTGAGGTGGGTGAACTCTGTGGTCAGAGAGGAATGGACGGCAGAAGTGTTCGATGTAGAGCTTTTAAAGTATCCCAACATAGAGGAGGAAATGGTAGAGATGTTACAAATAGGAATGTCTTGCGTCGCAAGAATGCCGGAGCAACGACCAAAGATGCAGGATGTGGTGAAAAGGGTGGAGGAAATTCGTCAAGTAAACACCGGAACTCGACCATCATCAGGAATTTCGACTCCAATCTTAAATACGCCGCCTCCTCCATTGGAGATAGCAGGACCTTCCTCTGTAACCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

630

Amino Acids

68.96

Weight (kDa)

6.88

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 62 2.2e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 79 - 127 6.5e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 324 - 590 1.1e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 324 - 589 5.3e-31 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1493, 1512
AccII CGCG 1 cut(s) 124
AciI CCGC 7 cut(s) 65, 756, 808, 1137, 1250, 1486, 1844
AclI AACGTT 1 cut(s) 989
AcsI RAATTY 7 cut(s) 145, 400, 481, 534, 1085, 1779, 1818
AdeI CACNNNGTG 1 cut(s) 1597
AfaI GTAC 1 cut(s) 1450
AfiI CCNNNNNNNGG 4 cut(s) 271, 814, 1143, 1814
AgeI ACCGGT 1 cut(s) 1547
AjnI CCWGG 2 cut(s) 164, 220
AjuI GAANNNNNNNTTGG 2 cut(s) 896, 928
AleI CACNNNNGTG 1 cut(s) 1043
Alw26I GTCTC 1 cut(s) 1256
Ama87I CYCGRG 1 cut(s) 1535
AoxI GGCC 3 cut(s) 385, 633, 1353
ApeKI GCWGC 5 cut(s) 227, 804, 1010, 1093, 1289
ApoI RAATTY 7 cut(s) 145, 400, 481, 534, 1085, 1779, 1818
AsiGI ACCGGT 1 cut(s) 1547
Asp700I GAANNNNTTC 1 cut(s) 1587
AspLEI GCGC 3 cut(s) 126, 277, 1208
AspS9I GGNCC 7 cut(s) 67, 239, 314, 386, 633, 1525, 1868
AsuHPI GGTGA 7 cut(s) 482, 542, 1060, 1537, 1609, 1618, 1774
AsuII TTCGAA 1 cut(s) 1160
AvaI CYCGRG 1 cut(s) 1535
AvaII GGWCC 5 cut(s) 67, 239, 314, 1525, 1868
BaeI ACNNNNGTAYC 2 cut(s) 1375, 1408
BanII GRGCYC 1 cut(s) 1468
BbsI GAAGAC 1 cut(s) 345
BbvCI CCTCAGC 1 cut(s) 969
BbvI GCAGC 5 cut(s) 214, 791, 997, 1105, 1301
BccI CCATC 2 cut(s) 1568, 1816
BceAI ACGGC 2 cut(s) 187, 1650
BciT130I CCWGG 2 cut(s) 166, 222
BciVI GTATCC 4 cut(s) 892, 1376, 1451, 1677
BcoDI GTCTC 1 cut(s) 1256
BfaI CTAG 2 cut(s) 555, 740
BfmI CTRYAG 2 cut(s) 181, 1513
BfoI RGCGCY 2 cut(s) 278, 1209
BfuI GTATCC 4 cut(s) 892, 1376, 1451, 1677
BisI GCNGC 8 cut(s) 228, 805, 808, 1011, 1094, 1137, 1290, 1844
BlsI GCNGC 8 cut(s) 229, 806, 809, 1012, 1095, 1138, 1291, 1845
Bme1390I CCNGG 2 cut(s) 166, 222
Bme18I GGWCC 5 cut(s) 67, 239, 314, 1525, 1868
BmeT110I CYCGRG 1 cut(s) 1535
BmgT120I GGNCC 7 cut(s) 67, 239, 314, 386, 633, 1525, 1868
BmiI GGNNCC 2 cut(s) 69, 1526
BmrFI CCNGG 2 cut(s) 166, 222
BmrI ACTGGG 3 cut(s) 65, 1267, 1879
BmsI GCATC 5 cut(s) 665, 971, 1012, 1511, 1740
BmuI ACTGGG 3 cut(s) 65, 1267, 1879
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 2 cut(s) 243, 654
Bpu10I CCTNAGC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 1160
BpuEI CTTGAG 1 cut(s) 1362
BsaJI CCNNGG 1 cut(s) 1339
BsaWI WCCGGW 2 cut(s) 1547, 1796
BsaXI ACNNNNNCTCC 2 cut(s) 1831, 1861
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 814, 1143, 1814
Bse118I RCCGGY 2 cut(s) 264, 1547
Bse1I ACTGG 3 cut(s) 71, 1262, 1885
BseBI CCWGG 2 cut(s) 166, 222
BseDI CCNNGG 1 cut(s) 1339
BseGI GGATG 3 cut(s) 764, 1560, 1762
BseLI CCNNNNNNNGG 4 cut(s) 271, 814, 1143, 1814
BseMII CTCAG 5 cut(s) 274, 606, 873, 960, 1331
BseNI ACTGG 3 cut(s) 71, 1262, 1885
BseRI GAGGAG 3 cut(s) 1248, 1694, 1839
BseXI GCAGC 5 cut(s) 214, 791, 997, 1105, 1301
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 1 cut(s) 1308
Bsh1236I CGCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 387, 635, 1355
BshTI ACCGGT 1 cut(s) 1547
BsiHKCI CYCGRG 1 cut(s) 1535
BsiSI CCGG 4 cut(s) 265, 1548, 1733, 1797
BslFI GGGAC 1 cut(s) 1009
BslI CCNNNNNNNGG 4 cut(s) 271, 814, 1143, 1814
BsmAI GTCTC 1 cut(s) 1256
BsmFI GGGAC 1 cut(s) 1009
BsmI GAATGC 1 cut(s) 1734
BsnI GGCC 3 cut(s) 387, 635, 1355
BsoBI CYCGRG 1 cut(s) 1535
Bsp119I TTCGAA 1 cut(s) 1160
Bsp1286I GDGCHC 1 cut(s) 1468
Bsp143I GATC 4 cut(s) 177, 190, 750, 940
Bsp19I CCATGG 1 cut(s) 1339
BspACI CCGC 7 cut(s) 65, 756, 808, 1137, 1250, 1486, 1844
BspANI GGCC 3 cut(s) 387, 635, 1355
BspCNI CTCAG 5 cut(s) 273, 605, 874, 961, 1330
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 2 cut(s) 69, 1526
BspQI GCTCTTC 1 cut(s) 681
BspT104I TTCGAA 1 cut(s) 1160
BsrFI RCCGGY 2 cut(s) 264, 1547
BsrI ACTGG 3 cut(s) 71, 1262, 1885
BssAI RCCGGY 2 cut(s) 264, 1547
BssECI CCNNGG 1 cut(s) 1339
BssMI GATC 4 cut(s) 177, 190, 750, 940
BssT1I CCWWGG 1 cut(s) 1339
Bst2UI CCWGG 2 cut(s) 166, 222
Bst4CI ACNGT 5 cut(s) 427, 499, 650, 1042, 1388
Bst6I CTCTTC 4 cut(s) 618, 681, 1046, 1216
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstBI TTCGAA 1 cut(s) 1160
BstC8I GCNNGC 3 cut(s) 87, 266, 732
BstDEI CTNAG 8 cut(s) 260, 287, 293, 592, 882, 969, 1142, 1317
BstDSI CCRYGG 1 cut(s) 1339
BstF5I GGATG 3 cut(s) 764, 1560, 1762
BstFNI CGCG 1 cut(s) 124
BstH2I RGCGCY 2 cut(s) 278, 1209
BstHHI GCGC 3 cut(s) 126, 277, 1208
BstKTI GATC 4 cut(s) 180, 193, 753, 943
BstMAI GTCTC 1 cut(s) 1256
BstMBI GATC 4 cut(s) 177, 190, 750, 940
BstMWI GCNNNNNNNGC 4 cut(s) 217, 274, 1031, 1145
BstNI CCWGG 2 cut(s) 166, 222
BstNSI RCATGY 2 cut(s) 59, 1226
BstSCI CCNGG 2 cut(s) 164, 220
BstSFI CTRYAG 2 cut(s) 181, 1513
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 5 cut(s) 214, 791, 997, 1105, 1301
BstV2I GAAGAC 1 cut(s) 345
BstXI CCANNNNNNTGG 1 cut(s) 1045
BsuI GTATCC 4 cut(s) 892, 1376, 1451, 1677
BsuRI GGCC 3 cut(s) 387, 635, 1355
BtgI CCRYGG 1 cut(s) 1339
BtgZI GCGATG 1 cut(s) 1413
BtsCI GGATG 3 cut(s) 764, 1560, 1762
BtsI GCAGTG 2 cut(s) 60, 1449
BtsIMutI CAGTG 4 cut(s) 60, 1038, 1415, 1449
Cac8I GCNNGC 3 cut(s) 87, 266, 732
CciI TCATGA 1 cut(s) 429
CfoI GCGC 3 cut(s) 126, 277, 1208
Cfr10I RCCGGY 2 cut(s) 264, 1547
Cfr13I GGNCC 7 cut(s) 67, 239, 314, 386, 633, 1525, 1868
CseI GACGC 1 cut(s) 1708
Csp6I GTAC 1 cut(s) 1449
CspAI ACCGGT 1 cut(s) 1547
CviAII CATG 5 cut(s) 56, 430, 1124, 1223, 1340
CviQI GTAC 1 cut(s) 1449
DdeI CTNAG 8 cut(s) 260, 287, 293, 592, 882, 969, 1142, 1317
DpnI GATC 4 cut(s) 179, 192, 752, 942
DpnII GATC 4 cut(s) 177, 190, 750, 940
DraI TTTAAA 2 cut(s) 399, 1662
DraIII CACNNNGTG 1 cut(s) 1597
Eam1104I CTCTTC 4 cut(s) 618, 681, 1046, 1216
EarI CTCTTC 4 cut(s) 618, 681, 1046, 1216
EciI GGCGGA 1 cut(s) 771
Eco130I CCWWGG 1 cut(s) 1339
Eco147I AGGCCT 1 cut(s) 1355
Eco24I GRGCYC 1 cut(s) 1468
Eco47I GGWCC 5 cut(s) 67, 239, 314, 1525, 1868
Eco88I CYCGRG 1 cut(s) 1535
EcoO109I RGGNCCY 2 cut(s) 1525, 1868
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 164, 220
EcoT14I CCWWGG 1 cut(s) 1339
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 1468
ErhI CCWWGG 1 cut(s) 1339
FaeI CATG 5 cut(s) 59, 433, 1127, 1226, 1343
FalI AAGNNNNNCTT 4 cut(s) 896, 928, 1195, 1227
FaqI GGGAC 1 cut(s) 1009
FatI CATG 5 cut(s) 55, 429, 1123, 1222, 1339
FauI CCCGC 1 cut(s) 1493
FblI GTMKAC 2 cut(s) 1493, 1512
Fnu4HI GCNGC 8 cut(s) 228, 805, 808, 1011, 1094, 1137, 1290, 1844
FokI GGATG 3 cut(s) 771, 1547, 1769
FriOI GRGCYC 1 cut(s) 1468
Fsp4HI GCNGC 8 cut(s) 228, 805, 808, 1011, 1094, 1137, 1290, 1844
FspBI CTAG 2 cut(s) 555, 740
GlaI GCGC 3 cut(s) 125, 276, 1207
GluI GCNGC 8 cut(s) 228, 805, 808, 1011, 1094, 1137, 1290, 1844
GsaI CCCAGC 1 cut(s) 1329
GsuI CTGGAG 2 cut(s) 243, 654
HaeII RGCGCY 2 cut(s) 278, 1209
HaeIII GGCC 3 cut(s) 387, 635, 1355
HapII CCGG 4 cut(s) 265, 1548, 1733, 1797
HgaI GACGC 1 cut(s) 1708
HhaI GCGC 3 cut(s) 126, 277, 1208
Hin1II CATG 5 cut(s) 59, 433, 1127, 1226, 1343
Hin6I GCGC 3 cut(s) 124, 275, 1206
HinP1I GCGC 3 cut(s) 124, 275, 1206
HindIII AAGCTT 3 cut(s) 87, 905, 1172
HpaII CCGG 4 cut(s) 265, 1548, 1733, 1797
HphI GGTGA 7 cut(s) 482, 542, 1060, 1537, 1609, 1618, 1774
Hpy166II GTNNAC 4 cut(s) 1494, 1513, 1609, 1792
Hpy188I TCNGA 6 cut(s) 328, 504, 883, 1320, 1507, 1622
Hpy188III TCNNGA 7 cut(s) 198, 430, 539, 944, 994, 1528, 1815
Hpy8I GTNNAC 4 cut(s) 1494, 1513, 1609, 1792
Hpy99I CGWCG 1 cut(s) 1724
HpyAV CCTTC 9 cut(s) 333, 477, 674, 812, 922, 1054, 1075, 1232, 1881
HpyCH4III ACNGT 5 cut(s) 427, 499, 650, 1042, 1388
HpyCH4IV ACGT 1 cut(s) 989
HpyCH4V TGCA 9 cut(s) 55, 449, 734, 935, 1025, 1034, 1289, 1502, 1753
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 274, 1031, 1145
HpyF3I CTNAG 8 cut(s) 260, 287, 293, 592, 882, 969, 1142, 1317
HpySE526I ACGT 1 cut(s) 989
Hsp92II CATG 5 cut(s) 59, 433, 1127, 1226, 1343
HspAI GCGC 3 cut(s) 124, 275, 1206
KroI GCCGGC 1 cut(s) 264
KroNI GCCGGC 1 cut(s) 266
Kzo9I GATC 4 cut(s) 177, 190, 750, 940
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 2 cut(s) 224, 1735
Lsp1109I GCAGC 5 cut(s) 214, 791, 997, 1105, 1301
LweI GCATC 5 cut(s) 665, 971, 1012, 1511, 1740
MaeI CTAG 2 cut(s) 555, 740
MaeII ACGT 1 cut(s) 989
MaeIII GTNAC 5 cut(s) 58, 1218, 1474, 1698, 1879
MalI GATC 4 cut(s) 179, 192, 752, 942
MboI GATC 4 cut(s) 177, 190, 750, 940
MfeI CAATTG 1 cut(s) 1281
MhlI GDGCHC 1 cut(s) 1468
MlyI GAGTC 5 cut(s) 481, 1258, 1263, 1432, 1819
MmeI TCCRAC 1 cut(s) 75
Mph1103I ATGCAT 1 cut(s) 451
MroNI GCCGGC 1 cut(s) 264
MroXI GAANNNNTTC 1 cut(s) 1587
MseI TTAA 5 cut(s) 333, 398, 1254, 1661, 1835
MslI CAYNNNNRTG 2 cut(s) 1043, 1128
MspI CCGG 4 cut(s) 265, 1548, 1733, 1797
MspR9I CCNGG 2 cut(s) 166, 222
MunI CAATTG 1 cut(s) 1281
Mva1269I GAATGC 1 cut(s) 1734
MvaI CCWGG 2 cut(s) 166, 222
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 4 cut(s) 217, 274, 1031, 1145
NaeI GCCGGC 1 cut(s) 266
NcoI CCATGG 1 cut(s) 1339
NdeII GATC 4 cut(s) 177, 190, 750, 940
NgoMIV GCCGGC 1 cut(s) 264
NlaIII CATG 5 cut(s) 59, 433, 1127, 1226, 1343
NlaIV GGNNCC 2 cut(s) 69, 1526
NmuCI GTSAC 2 cut(s) 58, 1474
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 59, 1226
NspV TTCGAA 1 cut(s) 1160
OliI CACNNNNGTG 1 cut(s) 1043
PaeR7I CTCGAG 1 cut(s) 1535
PagI TCATGA 1 cut(s) 429
PceI AGGCCT 1 cut(s) 1355
PciSI GCTCTTC 1 cut(s) 681
PctI GAATGC 1 cut(s) 1734
PdiI GCCGGC 1 cut(s) 266
PdmI GAANNNNTTC 1 cut(s) 1587
PfeI GAWTC 6 cut(s) 418, 433, 505, 720, 771, 1246
PinAI ACCGGT 1 cut(s) 1547
PkrI GCNGC 8 cut(s) 229, 806, 809, 1012, 1095, 1138, 1291, 1845
PleI GAGTC 5 cut(s) 481, 1258, 1263, 1431, 1819
PpsI GAGTC 5 cut(s) 481, 1258, 1263, 1431, 1819
PpuMI RGGWCCY 2 cut(s) 1525, 1868
Psp1406I AACGTT 1 cut(s) 989
Psp5II RGGWCCY 2 cut(s) 1525, 1868
Psp6I CCWGG 2 cut(s) 164, 220
PspFI CCCAGC 1 cut(s) 1325
PspGI CCWGG 2 cut(s) 164, 220
PspN4I GGNNCC 2 cut(s) 69, 1526
PspPI GGNCC 7 cut(s) 67, 239, 314, 386, 633, 1525, 1868
PspPPI RGGWCCY 2 cut(s) 1525, 1868
PspXI VCTCGAGB 1 cut(s) 1535
RsaI GTAC 1 cut(s) 1450
RsaNI GTAC 1 cut(s) 1449
RseI CAYNNNNRTG 2 cut(s) 1043, 1128
SapI GCTCTTC 1 cut(s) 681
SaqAI TTAA 5 cut(s) 333, 398, 1254, 1661, 1835
SatI GCNGC 8 cut(s) 228, 805, 808, 1011, 1094, 1137, 1290, 1844
Sau3AI GATC 4 cut(s) 177, 190, 750, 940
Sau96I GGNCC 7 cut(s) 67, 239, 314, 386, 633, 1525, 1868
SchI GAGTC 5 cut(s) 481, 1258, 1263, 1432, 1819
ScrFI CCNGG 2 cut(s) 166, 222
SduI GDGCHC 1 cut(s) 1468
SfaNI GCATC 5 cut(s) 665, 971, 1012, 1511, 1740
SfcI CTRYAG 2 cut(s) 181, 1513
Sfr274I CTCGAG 1 cut(s) 1535
SfuI TTCGAA 1 cut(s) 1160
SinI GGWCC 5 cut(s) 67, 239, 314, 1525, 1868
SlaI CTCGAG 1 cut(s) 1535
SmiMI CAYNNNNRTG 2 cut(s) 1043, 1128
SmlI CTYRAG 2 cut(s) 1377, 1535
SmoI CTYRAG 2 cut(s) 1377, 1535
SseBI AGGCCT 1 cut(s) 1355
SsiI CCGC 7 cut(s) 65, 756, 808, 1137, 1250, 1486, 1844
SspI AATATT 1 cut(s) 112
SspMI CTAG 2 cut(s) 555, 740
StuI AGGCCT 1 cut(s) 1355
StyD4I CCNGG 2 cut(s) 164, 220
StyI CCWWGG 1 cut(s) 1339
TaaI ACNGT 5 cut(s) 427, 499, 650, 1042, 1388
TaiI ACGT 1 cut(s) 992
TaqI TCGA 9 cut(s) 342, 473, 1160, 1214, 1267, 1536, 1647, 1804, 1823
TauI GCSGC 3 cut(s) 810, 1139, 1846
TfiI GAWTC 6 cut(s) 418, 433, 505, 720, 771, 1246
Tru1I TTAA 5 cut(s) 333, 398, 1254, 1661, 1835
Tru9I TTAA 5 cut(s) 333, 398, 1254, 1661, 1835
TscAI CASTG 4 cut(s) 67, 1045, 1422, 1449
TseFI GTSAC 2 cut(s) 58, 1474
TseI GCWGC 5 cut(s) 227, 804, 1010, 1093, 1289
Tsp45I GTSAC 2 cut(s) 58, 1474
TspDTI ATGAA 4 cut(s) 25, 91, 203, 446
TspRI CASTG 4 cut(s) 67, 1045, 1422, 1449
VpaK11BI GGWCC 5 cut(s) 67, 239, 314, 1525, 1868
XapI RAATTY 7 cut(s) 145, 400, 481, 534, 1085, 1779, 1818
XceI RCATGY 2 cut(s) 59, 1226
XhoI CTCGAG 1 cut(s) 1535
XmiI GTMKAC 2 cut(s) 1493, 1512
XmnI GAANNNNTTC 1 cut(s) 1587
XspI CTAG 2 cut(s) 555, 740
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.