pycom01g22480
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009719
Physical Location & Seq
Forward (+)
20059152 .. 20061133
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22480.1

Sequence Viewer

Length: 1875 bp
ATGGAGAAAAGATTAGAGCTTTTGGTCGTTTTGGTTACTGGGGCAATTTTCTTGCATGTCACTGCGGACCCAGTTGATGATAAGCAAGCCTTGTTTGATTTCGTTCACAACATTTCTCACTCAACCTCTATCAAATGGAATGAGAATTCTTCTGTTTGCAAAACCTGGACCGGCGTGGTCTGTAGCAAAGATCAGTCCGGAGTTATAGAGCTTCATTTGCCTGGAGCTGCCTTATATGGTCCAATCCCACCGAGCACTCTCAGCCGCCTTTCAGCGCTTCAGGTTCTAAATCTCAGTTCGAATACCTTGACAGGTCCATTCCCTTCTGATTTTTCCAAACTCAGAAACTTGTCTTCCCTTTGTCTTCAGTACAACAATTTCTCCGGCCCATTGCCTTTAGATTTTTCGGTTTGGAAGAATCTTACTGTCATGAATCTCTCAAACAATGCATTTAGTGGGAATATCCCTTCCTCGATTTCAAGTTTGACTCACCTCACAGTTCTGAATCTCGCTAACAATTGGCTTTCGGGTGAAATTCCTGACCTCAATGTTCCTAGTTTGCAGCAGCTTGATTTGGCTAACAATAACCTCTCAGGGAATGTGCCTCAATCTCTTCAGAAATTTCCAAGTTGGGCCTTTTCAGGCAACACTCTTGCGTTCCTTCCTCCAGCTCTTCCGGTTCAACCACCTAATTCTAAACCGAGGAAGAAAGTGAAACTCGGCAAGCATGCAATGCTAGGTATTGTGATTGGTGGATGTGTTCTGGGATTTGTTTTAATTGCTGTTCTGATGATCATTTGCCGTTACAACAAAAAAGGTGAAAATGGGATAGTTGAAAAACCAAAGAAGGAACTCTTTTCAAAGGAAGGGCTTTCTCGGAAGCATGATAAGAATAACAGGATTTCGTTCTTCGAGGGTTCTAATCTTGCATTTGATCTGGAGGACTTGTTGAGGGCGTCTGCTGACGTTCTTGGCAAGGGAACGTTTGGGACGACATATAAGGCAGCTTTAGAAGATGCAACTACTGTTGTGGTGAAGAGGTTGAAGGAAGTGAGTGTGGGAAAAAAGGAATTTGAGCAGCAAATAGAGATTGTGGGAAGTATCAGGCATGAGAATGCAGCCGCGCTGAGGGCGTATTACTATTCAAAAGATGAGAAGCTTGTTGTTTATGATTACTATGAGCAGGGAAGCGCCTCGTCGCTGTTACATGCTAAAAGAGGAGAGGGCAGGATTCCACTCGACTGGGAGACTCGACTGAAAATAGCAATCGGTGCAGCAAGAGGCATCGCTCACATCCACACGCAGAATGGTGGGAAACTTGTCCATGGAAACATAAAGGCCTCAAACATCTTCCTCAACTCTCAAGGATACGGTTGTGTGTGCGATGTTGGTTTGCCAACACTGATGGGTCCAATGCCCCCACCAGCAGCGCGTACTGGAGGATACCGAGCCCCGGAAGTGAAAGACACCCGTAAGTCTTCCCCTGCATCTGATGTCTACAGTTTCGGGGTCCTGGTACTCGAGCTTCTCACCGGAAAAAGCCCCATACACACAATAGGTGGCGAAGAAGTTGTTCACTTGGTCCGGTGGGTGAACTCTGTGGTCAGAGAGGAATGGACAGCAGAAGTGTTTGACGTGGAGCTTTTGAGGTATCCAAACATAGAGGAGGAAATGGTAGAGATGTTACAAATAGGAATGTCTTGTGTGGCAAGAATGCCTGAGCAACGACCAAATATGCCGGATTTGGTGAAAAGGGTGGAAGAAATTCGTCAAGTAAACACCGGAACTCGACCATCATCAGGAATATCTACTCCAATCTTAAATACGCCGACTCCTCCATCGGAGACAGCAGGACCTTCCTTTCTACCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

625

Amino Acids

68.27

Weight (kDa)

8.44

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 62 1.5e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 82 - 127 3.5e-08 Leucine rich repeat
LRR_4 PF12799 163 - 201 7.6e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 322 - 585 3.6e-31 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 322 - 584 4.2e-29 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1497
AccII CGCG 2 cut(s) 1124, 1432
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 3 cut(s) 65, 265, 1122
AclI AACGTT 1 cut(s) 983
AcsI RAATTY 5 cut(s) 145, 534, 620, 1070, 1764
AcuI CTGAAG 3 cut(s) 263, 350, 599
AcyI GRCGYC 1 cut(s) 956
AfaI GTAC 3 cut(s) 371, 1435, 1518
AfeI AGCGCT 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 1128, 1453, 1799
AgsI TTSAA 6 cut(s) 480, 683, 836, 861, 1045, 1146
AjiI CACGTC 1 cut(s) 1636
AjnI CCWGG 3 cut(s) 164, 220, 1512
AluBI AGCT 9 cut(s) 19, 211, 227, 568, 671, 1007, 1159, 1525, 1642
AluI AGCT 9 cut(s) 19, 211, 227, 568, 671, 1007, 1159, 1525, 1642
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 2 cut(s) 1241, 1838
Ama87I CYCGRG 1 cut(s) 1520
Aor13HI TCCGGA 1 cut(s) 197
Aor51HI AGCGCT 1 cut(s) 276
AoxI GGCC 3 cut(s) 385, 633, 1338
ApeKI GCWGC 8 cut(s) 227, 562, 565, 1004, 1078, 1118, 1274, 1427
ApoI RAATTY 5 cut(s) 145, 534, 620, 1070, 1764
Asp700I GAANNNNTTC 2 cut(s) 1572, 1764
AspLEI GCGC 4 cut(s) 277, 1126, 1193, 1432
AsuC2I CCSGG 1 cut(s) 1454
AsuHPI GGTGA 7 cut(s) 482, 542, 830, 1045, 1522, 1603, 1759
AsuII TTCGAA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 1520
AvaII GGWCC 8 cut(s) 67, 168, 239, 314, 1409, 1510, 1582, 1853
BaeI ACNNNNGTAYC 2 cut(s) 1360, 1393
BanII GRGCYC 1 cut(s) 1453
BarI GAAGNNNNNNTAC 2 cut(s) 1509, 1541
BbsI GAAGAC 3 cut(s) 345, 356, 1470
Bbv12I GWGCWC 1 cut(s) 257
BbvCI CCTCAGC 1 cut(s) 1127
BbvI GCAGC 8 cut(s) 214, 574, 577, 1016, 1090, 1130, 1286, 1439
BccI CCATC 3 cut(s) 1399, 1801, 1846
BceAI ACGGC 1 cut(s) 786
BciT130I CCWGG 3 cut(s) 166, 222, 1514
BciVI GTATCC 3 cut(s) 1361, 1436, 1662
BclI TGATCA 1 cut(s) 792
BcnI CCSGG 1 cut(s) 1454
BcoDI GTCTC 2 cut(s) 1241, 1838
BfaI CTAG 2 cut(s) 555, 737
BfmI CTRYAG 2 cut(s) 181, 1498
BfoI RGCGCY 2 cut(s) 278, 1194
BfuI GTATCC 3 cut(s) 1361, 1436, 1662
Bme1390I CCNGG 4 cut(s) 166, 222, 1454, 1514
Bme18I GGWCC 8 cut(s) 67, 168, 239, 314, 1409, 1510, 1582, 1853
BmeT110I CYCGRG 1 cut(s) 1520
BmgBI CACGTC 1 cut(s) 1636
BmiI GGNNCC 3 cut(s) 69, 1410, 1511
BmrFI CCNGG 4 cut(s) 166, 222, 1454, 1514
BmrI ACTGGG 4 cut(s) 48, 65, 1252, 1864
BmsI GCATC 3 cut(s) 1006, 1293, 1496
BmuI ACTGGG 4 cut(s) 48, 65, 1252, 1864
BpiI GAAGAC 3 cut(s) 345, 356, 1470
BpmI CTGGAG 4 cut(s) 243, 651, 959, 1458
Bpu10I CCTNAGC 2 cut(s) 1127, 1719
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 1347
BpuMI CCSGG 1 cut(s) 1454
BsaHI GRCGYC 1 cut(s) 956
BsaJI CCNNGG 3 cut(s) 701, 1324, 1452
BsaWI WCCGGW 5 cut(s) 197, 676, 1532, 1584, 1781
BsaXI ACNNNNNCTCC 6 cut(s) 365, 395, 932, 962, 1816, 1846
Bsc4I CCNNNNNNNGG 3 cut(s) 1128, 1453, 1799
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 5 cut(s) 43, 71, 1247, 1441, 1870
Bse3DI GCAATG 2 cut(s) 389, 738
BseAI TCCGGA 1 cut(s) 197
BseBI CCWGG 3 cut(s) 166, 222, 1514
BseDI CCNNGG 3 cut(s) 701, 1324, 1452
BseGI GGATG 2 cut(s) 761, 1293
BseLI CCNNNNNNNGG 3 cut(s) 1128, 1453, 1799
BseMI GCAATG 2 cut(s) 389, 738
BseMII CTCAG 6 cut(s) 274, 307, 355, 606, 1118, 1710
BseNI ACTGG 5 cut(s) 43, 71, 1247, 1441, 1870
BseRI GAGGAG 3 cut(s) 1233, 1679, 1824
BseXI GCAGC 8 cut(s) 214, 574, 577, 1016, 1090, 1130, 1286, 1439
BsgI GTGCAG 1 cut(s) 1293
Bsh1236I CGCG 2 cut(s) 1124, 1432
BshFI GGCC 3 cut(s) 387, 635, 1340
BsiHKAI GWGCWC 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 1520
BsiSI CCGG 9 cut(s) 171, 198, 384, 677, 1454, 1533, 1585, 1739, 1782
BslFI GGGAC 1 cut(s) 1003
BslI CCNNNNNNNGG 3 cut(s) 1128, 1453, 1799
BsmAI GTCTC 2 cut(s) 1241, 1838
BsmFI GGGAC 1 cut(s) 1003
BsmI GAATGC 2 cut(s) 1120, 1719
BsnI GGCC 3 cut(s) 387, 635, 1340
BsoBI CYCGRG 1 cut(s) 1520
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 257, 1453
Bsp13I TCCGGA 1 cut(s) 197
Bsp143I GATC 3 cut(s) 190, 792, 934
Bsp19I CCATGG 1 cut(s) 1324
BspACI CCGC 3 cut(s) 65, 265, 1122
BspANI GGCC 3 cut(s) 387, 635, 1340
BspCNI CTCAG 6 cut(s) 273, 306, 354, 605, 1119, 1711
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 2 cut(s) 1124, 1432
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 3 cut(s) 69, 1410, 1511
BspQI GCTCTTC 1 cut(s) 678
BspT104I TTCGAA 1 cut(s) 299
BsrDI GCAATG 2 cut(s) 389, 738
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 5 cut(s) 43, 71, 1247, 1441, 1870
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 701, 1324, 1452
BssMI GATC 3 cut(s) 190, 792, 934
BssNI GRCGYC 1 cut(s) 956
BssT1I CCWWGG 1 cut(s) 1324
Bst2UI CCWGG 3 cut(s) 166, 222, 1514
Bst4CI ACNGT 5 cut(s) 427, 499, 1027, 1373, 1502
Bst6I CTCTTC 3 cut(s) 618, 678, 1031
BstACI GRCGYC 1 cut(s) 956
BstAPI GCANNNNNTGC 2 cut(s) 733, 1271
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 3 cut(s) 87, 725, 729
BstDEI CTNAG 6 cut(s) 260, 293, 341, 592, 1127, 1719
BstDSI CCRYGG 1 cut(s) 1324
BstF5I GGATG 2 cut(s) 761, 1293
BstFNI CGCG 2 cut(s) 1124, 1432
BstH2I RGCGCY 2 cut(s) 278, 1194
BstHHI GCGC 4 cut(s) 277, 1126, 1193, 1432
BstKTI GATC 3 cut(s) 193, 795, 937
BstMAI GTCTC 2 cut(s) 1241, 1838
BstMBI GATC 3 cut(s) 190, 792, 934
BstMWI GCNNNNNNNGC 5 cut(s) 217, 261, 733, 1130, 1271
BstNI CCWGG 3 cut(s) 166, 222, 1514
BstNSI RCATGY 3 cut(s) 59, 731, 1211
BstSCI CCNGG 4 cut(s) 164, 220, 1452, 1512
BstSFI CTRYAG 2 cut(s) 181, 1498
BstUI CGCG 2 cut(s) 1124, 1432
BstV1I GCAGC 8 cut(s) 214, 574, 577, 1016, 1090, 1130, 1286, 1439
BstV2I GAAGAC 3 cut(s) 345, 356, 1470
BstXI CCANNNNNNTGG 1 cut(s) 1242
BsuI GTATCC 3 cut(s) 1361, 1436, 1662
BsuRI GGCC 3 cut(s) 387, 635, 1340
BtgI CCRYGG 1 cut(s) 1324
BtgZI GCGATG 2 cut(s) 1270, 1398
BtrI CACGTC 1 cut(s) 1636
BtsCI GGATG 2 cut(s) 761, 1293
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 60, 1400
Cac8I GCNNGC 3 cut(s) 87, 725, 729
CciI TCATGA 1 cut(s) 429
CfoI GCGC 4 cut(s) 277, 1126, 1193, 1432
Cfr10I RCCGGY 1 cut(s) 170
CseI GACGC 1 cut(s) 945
Csp6I GTAC 3 cut(s) 370, 1434, 1517
CviAII CATG 7 cut(s) 56, 430, 728, 884, 1109, 1208, 1325
CviQI GTAC 3 cut(s) 370, 1434, 1517
DdeI CTNAG 6 cut(s) 260, 293, 341, 592, 1127, 1719
DpnI GATC 3 cut(s) 192, 794, 936
DpnII GATC 3 cut(s) 190, 792, 934
Eam1104I CTCTTC 3 cut(s) 618, 678, 1031
EarI CTCTTC 3 cut(s) 618, 678, 1031
Eco130I CCWWGG 1 cut(s) 1324
Eco147I AGGCCT 1 cut(s) 1340
Eco24I GRGCYC 1 cut(s) 1453
Eco47I GGWCC 8 cut(s) 67, 168, 239, 314, 1409, 1510, 1582, 1853
Eco47III AGCGCT 1 cut(s) 276
Eco57I CTGAAG 3 cut(s) 263, 350, 599
Eco88I CYCGRG 1 cut(s) 1520
EcoO109I RGGNCCY 2 cut(s) 1510, 1853
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 164, 220, 1512
EcoT14I CCWWGG 1 cut(s) 1324
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 1453
ErhI CCWWGG 1 cut(s) 1324
FaeI CATG 7 cut(s) 59, 433, 731, 887, 1112, 1211, 1328
FalI AAGNNNNNCTT 4 cut(s) 74, 106, 839, 871
FaqI GGGAC 1 cut(s) 1003
FatI CATG 7 cut(s) 55, 429, 727, 883, 1108, 1207, 1324
FbaI TGATCA 1 cut(s) 792
FblI GTMKAC 1 cut(s) 1497
FokI GGATG 2 cut(s) 768, 1280
FriOI GRGCYC 1 cut(s) 1453
FspBI CTAG 2 cut(s) 555, 737
GlaI GCGC 4 cut(s) 276, 1125, 1192, 1431
GsuI CTGGAG 4 cut(s) 243, 651, 959, 1458
HaeII RGCGCY 2 cut(s) 278, 1194
HaeIII GGCC 3 cut(s) 387, 635, 1340
HapII CCGG 9 cut(s) 171, 198, 384, 677, 1454, 1533, 1585, 1739, 1782
HgaI GACGC 1 cut(s) 945
HhaI GCGC 4 cut(s) 277, 1126, 1193, 1432
Hin1I GRCGYC 1 cut(s) 956
Hin1II CATG 7 cut(s) 59, 433, 731, 887, 1112, 1211, 1328
Hin6I GCGC 4 cut(s) 275, 1124, 1191, 1430
HinP1I GCGC 4 cut(s) 275, 1124, 1191, 1430
HindIII AAGCTT 1 cut(s) 1157
HinfI GANTC 7 cut(s) 418, 433, 487, 505, 1231, 1249, 1831
HpaII CCGG 9 cut(s) 171, 198, 384, 677, 1454, 1533, 1585, 1739, 1782
HphI GGTGA 7 cut(s) 482, 542, 830, 1045, 1522, 1603, 1759
Hpy166II GTNNAC 5 cut(s) 106, 1498, 1576, 1594, 1777
Hpy188I TCNGA 9 cut(s) 328, 344, 504, 618, 789, 879, 1492, 1607, 1843
Hpy188III TCNNGA 5 cut(s) 198, 430, 539, 938, 1800
Hpy8I GTNNAC 5 cut(s) 106, 1498, 1576, 1594, 1777
Hpy99I CGWCG 1 cut(s) 1201
HpyAV CCTTC 7 cut(s) 333, 477, 671, 841, 860, 1039, 1866
HpyCH4III ACNGT 5 cut(s) 427, 499, 1027, 1373, 1502
HpyCH4IV ACGT 3 cut(s) 966, 983, 1635
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 261, 733, 1130, 1271
HpyF3I CTNAG 6 cut(s) 260, 293, 341, 592, 1127, 1719
HpySE526I ACGT 3 cut(s) 966, 983, 1635
Hsp92I GRCGYC 1 cut(s) 956
Hsp92II CATG 7 cut(s) 59, 433, 731, 887, 1112, 1211, 1328
HspAI GCGC 4 cut(s) 275, 1124, 1191, 1430
Kpn2I TCCGGA 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 792
Kzo9I GATC 3 cut(s) 190, 792, 934
LguI GCTCTTC 1 cut(s) 678
LmnI GCTCC 2 cut(s) 224, 1639
Lsp1109I GCAGC 8 cut(s) 214, 574, 577, 1016, 1090, 1130, 1286, 1439
LweI GCATC 3 cut(s) 1006, 1293, 1496
MaeI CTAG 2 cut(s) 555, 737
MaeII ACGT 3 cut(s) 966, 983, 1635
MaeIII GTNAC 5 cut(s) 34, 58, 803, 1203, 1683
MalI GATC 3 cut(s) 192, 794, 936
MboI GATC 3 cut(s) 190, 792, 934
MfeI CAATTG 1 cut(s) 517
MhlI GDGCHC 2 cut(s) 257, 1453
MlyI GAGTC 3 cut(s) 481, 1243, 1825
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 1 cut(s) 197
MroXI GAANNNNTTC 2 cut(s) 1572, 1764
MseI TTAA 2 cut(s) 776, 1820
MslI CAYNNNNRTG 1 cut(s) 1113
MspI CCGG 9 cut(s) 171, 198, 384, 677, 1454, 1533, 1585, 1739, 1782
MspR9I CCNGG 4 cut(s) 166, 222, 1454, 1514
MunI CAATTG 1 cut(s) 517
Mva1269I GAATGC 2 cut(s) 1120, 1719
MvaI CCWGG 3 cut(s) 166, 222, 1514
MvnI CGCG 2 cut(s) 1124, 1432
MwoI GCNNNNNNNGC 5 cut(s) 217, 261, 733, 1130, 1271
NciI CCSGG 1 cut(s) 1454
NcoI CCATGG 1 cut(s) 1324
NdeII GATC 3 cut(s) 190, 792, 934
NlaIII CATG 7 cut(s) 59, 433, 731, 887, 1112, 1211, 1328
NlaIV GGNNCC 3 cut(s) 69, 1410, 1511
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 3 cut(s) 59, 731, 1211
NspV TTCGAA 1 cut(s) 299
PaeI GCATGC 1 cut(s) 731
PaeR7I CTCGAG 1 cut(s) 1520
PagI TCATGA 1 cut(s) 429
PceI AGGCCT 1 cut(s) 1340
PciSI GCTCTTC 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 989
PctI GAATGC 2 cut(s) 1120, 1719
PdmI GAANNNNTTC 2 cut(s) 1572, 1764
PfeI GAWTC 4 cut(s) 418, 433, 505, 1231
PleI GAGTC 3 cut(s) 481, 1243, 1825
PpsI GAGTC 3 cut(s) 481, 1243, 1825
PpuMI RGGWCCY 2 cut(s) 1510, 1853
Psp1406I AACGTT 1 cut(s) 983
Psp5II RGGWCCY 2 cut(s) 1510, 1853
Psp6I CCWGG 3 cut(s) 164, 220, 1512
PspGI CCWGG 3 cut(s) 164, 220, 1512
PspN4I GGNNCC 3 cut(s) 69, 1410, 1511
PspPPI RGGWCCY 2 cut(s) 1510, 1853
PspXI VCTCGAGB 1 cut(s) 1520
RsaI GTAC 3 cut(s) 371, 1435, 1518
RsaNI GTAC 3 cut(s) 370, 1434, 1517
RseI CAYNNNNRTG 1 cut(s) 1113
SapI GCTCTTC 1 cut(s) 678
SaqAI TTAA 2 cut(s) 776, 1820
Sau3AI GATC 3 cut(s) 190, 792, 934
SchI GAGTC 3 cut(s) 481, 1243, 1825
ScrFI CCNGG 4 cut(s) 166, 222, 1454, 1514
SduI GDGCHC 2 cut(s) 257, 1453
SfaNI GCATC 3 cut(s) 1006, 1293, 1496
SfcI CTRYAG 2 cut(s) 181, 1498
Sfr274I CTCGAG 1 cut(s) 1520
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 8 cut(s) 67, 168, 239, 314, 1409, 1510, 1582, 1853
SlaI CTCGAG 1 cut(s) 1520
SmiMI CAYNNNNRTG 1 cut(s) 1113
SmlI CTYRAG 2 cut(s) 1362, 1520
SmoI CTYRAG 2 cut(s) 1362, 1520
SphI GCATGC 1 cut(s) 731
SseBI AGGCCT 1 cut(s) 1340
SsiI CCGC 3 cut(s) 65, 265, 1122
SspMI CTAG 2 cut(s) 555, 737
StuI AGGCCT 1 cut(s) 1340
StyD4I CCNGG 4 cut(s) 164, 220, 1452, 1512
StyI CCWWGG 1 cut(s) 1324
TaaI ACNGT 5 cut(s) 427, 499, 1027, 1373, 1502
TaiI ACGT 3 cut(s) 969, 986, 1638
TaqI TCGA 7 cut(s) 299, 473, 912, 1239, 1252, 1521, 1789
TatI WGTACW 1 cut(s) 369
TauI GCSGC 2 cut(s) 267, 1124
TfiI GAWTC 4 cut(s) 418, 433, 505, 1231
Tru1I TTAA 2 cut(s) 776, 1820
Tru9I TTAA 2 cut(s) 776, 1820
TscAI CASTG 2 cut(s) 67, 1407
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 8 cut(s) 227, 562, 565, 1004, 1078, 1118, 1274, 1427
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 2 cut(s) 203, 446
TspRI CASTG 2 cut(s) 67, 1407
VpaK11BI GGWCC 8 cut(s) 67, 168, 239, 314, 1409, 1510, 1582, 1853
XapI RAATTY 5 cut(s) 145, 534, 620, 1070, 1764
XceI RCATGY 3 cut(s) 59, 731, 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 1304
XhoI CTCGAG 1 cut(s) 1520
XmiI GTMKAC 1 cut(s) 1497
XmnI GAANNNNTTC 2 cut(s) 1572, 1764
XspI CTAG 2 cut(s) 555, 737
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.