MD02G1004000.v1.1

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012722
Physical Location & Seq
Reverse (-)
283420 .. 287361
3942 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1004000.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGAGGGAGGAGGGCGATCGGCGGACACGATGATGTCAGAGGCGGCGCCACCGTCGCAACCGGACCCTAACCACCCACAGCAACATCCTCCACCGCCACATCAGGCGATGGGGGGAGTGGAGAGCATTCCGGCCACGCTCAGCCACGGCGGCCGATTCATTCAGTACAATATCTTCGGCAACGTCTTTGAGGTCACCGCCAAGTACAAGCCCCCCATCATGCCCATCGGTAAAGGCGCATACGGCATCGTTTGCTCGGCTCTGAATTCGGAGACGAACGAGCACGTTGCGGTAAAGAAGATTGCGAATGCATTCGACAACAGGATCGATGCGAAGAGGACGCTGAGAGAGATCAAGCTGCTGCGACATATGGATCATGAGAATGTTGTTGCGATTCGGGATATAATCCCTCCGCCGCAGAGGAACTTCTTCAATGATGTGTACATTGCGTATGAGCTCATGGACACTGACCTCCATCAAATCATTCGATCCAATCAAGCTCTATCCGAGGAGCACTGTCAGTATTTCTTATATCAGATCCTCCGAGGATTGAAATACATTCACTCTGCAAATGTTCTGCACAGGGACTTAAAACCTAGCAATCTTCTCCTAAATGCCAATTGTGACTTAAAAATATGCGATTTTGGACTAGCTCGAGTCACCTCAGAGACTGATTTTATGACTGAGTATGTTGTTACCAGATGGTACCGTGCCCCAGAGCTACTGTTAAACTCTTCAGATTACACTGCAGCTATTGATGTATGGTCAGTAGGTTGTATATTTATGGAATTGATGGATAGGAAGCCATTATTTCCTGGCAGAGATCACGTGCATCAGCTTCGTCTGCTTATGGAGCTGATTGGCACCCCATCAGAGGCTGAGCTGCAATTTCTAAATGAAAATGCGAAGAGATACATTCGGCAGCTTCCTCTCTACCGTCGACAGTCATTTACCGAGAAGTTTCCTCACGTCCATCCTTCAGCAATTGATCTTGTTGAAAAGATGTTGACATTTGATCCTACTCGGAGAATTACTGTTGAAGATGCCCTAGCTCACCCCTATTTAACATCTCTCCATGACATCAGTGATGAGCCTATTTGCTTGACTCCCTTCAGCTTCGACTTTGAGCAGCATGCACTCTCTGAGGAACAGATGAAAGAGCTGATCTACCGAGAGGCACTTGCATTTAACCCCGAGTACCAGCGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000302 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005802 GO:0005856 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009314 GO:0009403 GO:0009404 GO:0009409 GO:0009411 GO:0009416 GO:0009524 GO:0009555 GO:0009574 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009682 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009791 GO:0009856 GO:0009864 GO:0009867 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010150 GO:0010183 GO:0010224 GO:0010229 GO:0010468 GO:0012505 GO:0015630 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0022414 GO:0022622 GO:0023014 GO:0023052 GO:0031347 GO:0031349 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033993 GO:0034641 GO:0035556 GO:0035670 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042430 GO:0042435 GO:0042493 GO:0042542 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0044706 GO:0045087 GO:0045088 GO:0045089 GO:0046217 GO:0046483 GO:0046677 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0050918 GO:0051301 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0080134 GO:0080135 GO:0080136 GO:0090567 GO:0090693 GO:0097305 GO:0098542 GO:0098791 GO:0099402 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Pkinase

Protein Analysis

404

Amino Acids

46.27

Weight (kDa)

5.83

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 75 - 355 9.6e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 76 - 266 8.1e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012722)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43790
fragaria_vesca FvH4_1g00480 FvH4_1g00480
malus_domestica MD02G1004000.v1.1 MD15G1147300.v1.1
prunus_persica Prupe.7G267300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084991
rosa_laevigata RLG00000015654
rosa_multiflora Rmu_co8283871.1_g000001 Rmu_sc0001196.1_g000005 Rmu_sc0008647.1_g000018
rosa_roxburghii Rroxscaffold_2G00155650
rosa_rugosa Rorug01G0457600
rosa_samantha Rh2AG005500 Rh2BG006500 Rh2CG007000 Rh2DG006900
rosa_wichuraiana Rw2G000520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 705
AccB1I GGYRCC 3 cut(s) 46, 705, 863
AccI GTMKAC 1 cut(s) 940
AciI CCGC 8 cut(s) 23, 44, 95, 150, 198, 290, 413, 416
AclWI GGATC 5 cut(s) 332, 381, 483, 532, 1010
AcoI YGGCCR 2 cut(s) 132, 151
AcsI RAATTY 1 cut(s) 265
AcuI CTGAAG 3 cut(s) 720, 963, 1096
AcvI CACGTG 1 cut(s) 829
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 5 cut(s) 167, 206, 443, 707, 1199
AfiI CCNNNNNNNGG 1 cut(s) 874
AgsI TTSAA 4 cut(s) 433, 553, 998, 1040
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 1 cut(s) 814
Alw21I GWGCWC 3 cut(s) 285, 459, 516
Alw26I GTCTC 2 cut(s) 266, 662
AlwI GGATC 5 cut(s) 332, 381, 483, 532, 1010
AlwNI CAGNNNCTG 2 cut(s) 671, 878
Ama87I CYCGRG 2 cut(s) 654, 1193
AoxI GGCC 2 cut(s) 132, 151
ApeKI GCWGC 6 cut(s) 358, 361, 749, 883, 922, 1129
ApoI RAATTY 1 cut(s) 265
Asp700I GAANNNNTTC 2 cut(s) 311, 428
Asp718I GGTACC 1 cut(s) 705
AspLEI GCGC 2 cut(s) 49, 239
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 3 cut(s) 187, 652, 1046
AvaI CYCGRG 2 cut(s) 654, 1193
AvaII GGWCC 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 715
BanI GGYRCC 3 cut(s) 46, 705, 863
BanII GRGCYC 1 cut(s) 459
BarI GAAGNNNNNNTAC 2 cut(s) 158, 190
BbrPI CACGTG 1 cut(s) 829
Bbv12I GWGCWC 3 cut(s) 285, 459, 516
BbvI GCAGC 6 cut(s) 345, 348, 761, 870, 934, 1141
BccI CCATC 8 cut(s) 103, 224, 233, 483, 696, 787, 877, 981
BceAI ACGGC 2 cut(s) 163, 259
BcgI CGANNNNNNTGC 4 cut(s) 269, 303, 821, 855
BciT130I CCWGG 1 cut(s) 816
BcoDI GTCTC 2 cut(s) 266, 662
BfaI CTAG 3 cut(s) 597, 650, 1049
BfmI CTRYAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 50
BglI GCCNNNNNGGC 1 cut(s) 150
BisI GCNGC 9 cut(s) 45, 151, 359, 362, 416, 750, 884, 923, 1130
BlpI GCTNAGC 2 cut(s) 140, 879
BlsI GCNGC 9 cut(s) 46, 152, 360, 363, 417, 751, 885, 924, 1131
Bme1390I CCNGG 1 cut(s) 816
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 2 cut(s) 654, 1193
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 4 cut(s) 48, 66, 707, 865
BmrFI CCNGG 1 cut(s) 816
BmsI GCATC 4 cut(s) 255, 319, 841, 1033
Bpu1102I GCTNAGC 2 cut(s) 140, 879
Bsa29I ATCGAT 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 829
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 145, 507, 544
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 1 cut(s) 874
Bse3DI GCAATG 1 cut(s) 444
BseBI CCWGG 1 cut(s) 816
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 3 cut(s) 145, 507, 544
BseGI GGATG 2 cut(s) 85, 973
BseLI CCNNNNNNNGG 1 cut(s) 874
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 6 cut(s) 154, 335, 675, 678, 870, 1134
BseRI GAGGAG 2 cut(s) 24, 524
BseSI GKGCMC 1 cut(s) 715
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 6 cut(s) 345, 348, 761, 870, 934, 1141
BsgI GTGCAG 1 cut(s) 563
Bsh1285I CGRYCG 2 cut(s) 20, 154
BshFI GGCC 2 cut(s) 134, 153
BshNI GGYRCC 3 cut(s) 46, 705, 863
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 2 cut(s) 20, 154
BsiHKAI GWGCWC 3 cut(s) 285, 459, 516
BsiHKCI CYCGRG 2 cut(s) 654, 1193
BsiSI CCGG 2 cut(s) 62, 131
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 1 cut(s) 874
BsmAI GTCTC 2 cut(s) 266, 662
BsmBI CGTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 599
BsmI GAATGC 3 cut(s) 126, 311, 313
BsnI GGCC 2 cut(s) 134, 153
BsoBI CYCGRG 2 cut(s) 654, 1193
Bsp1286I GDGCHC 4 cut(s) 285, 459, 516, 715
Bsp1407I TGTACA 1 cut(s) 441
Bsp1720I GCTNAGC 2 cut(s) 140, 879
BspACI CCGC 8 cut(s) 23, 44, 95, 150, 198, 290, 413, 416
BspANI GGCC 2 cut(s) 134, 153
BspCNI CTCAG 6 cut(s) 153, 336, 676, 677, 871, 1135
BspDI ATCGAT 1 cut(s) 327
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 4 cut(s) 48, 66, 707, 865
BspMAI CTGCAG 1 cut(s) 751
BspPI GGATC 5 cut(s) 332, 381, 483, 532, 1010
BspT107I GGYRCC 3 cut(s) 46, 705, 863
BsrDI GCAATG 1 cut(s) 444
BsrGI TGTACA 1 cut(s) 441
BssECI CCNNGG 3 cut(s) 145, 507, 544
BssNI GRCGYC 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 8 cut(s) 54, 518, 710, 726, 938, 945, 1036, 1209
Bst6I CTCTTC 3 cut(s) 329, 739, 902
BstACI GRCGYC 1 cut(s) 47
BstAPI GCANNNNNTGC 1 cut(s) 252
BstAUI TGTACA 1 cut(s) 441
BstBAI YACGTR 1 cut(s) 829
BstC8I GCNNGC 1 cut(s) 1134
BstDEI CTNAG 6 cut(s) 140, 344, 664, 684, 879, 1143
BstDSI CCRYGG 1 cut(s) 145
BstEII GGTNACC 1 cut(s) 193
BstF5I GGATG 2 cut(s) 85, 973
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 2 cut(s) 49, 239
BstMAI GTCTC 2 cut(s) 266, 662
BstMCI CGRYCG 2 cut(s) 20, 154
BstMWI GCNNNNNNNGC 6 cut(s) 55, 150, 243, 252, 844, 853
BstNI CCWGG 1 cut(s) 816
BstNSI RCATGY 1 cut(s) 1136
BstPI GGTNACC 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 814
BstSFI CTRYAG 1 cut(s) 747
BstSLI GKGCMC 1 cut(s) 715
BstV1I GCAGC 6 cut(s) 345, 348, 761, 870, 934, 1141
BstX2I RGATCY 1 cut(s) 537
BstYI RGATCY 1 cut(s) 537
BstZI CGGCCG 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 327
BsuRI GGCC 2 cut(s) 134, 153
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 122
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 2 cut(s) 85, 973
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 4 cut(s) 465, 514, 744, 1090
Cac8I GCNNGC 1 cut(s) 1134
CaiI CAGNNNCTG 2 cut(s) 671, 878
CciI TCATGA 1 cut(s) 376
CfoI GCGC 2 cut(s) 49, 239
Cfr13I GGNCC 1 cut(s) 64
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 1 cut(s) 349
Csp6I GTAC 5 cut(s) 166, 205, 442, 706, 1198
CviAII CATG 5 cut(s) 220, 377, 460, 1076, 1133
CviQI GTAC 5 cut(s) 166, 205, 442, 706, 1198
DdeI CTNAG 6 cut(s) 140, 344, 664, 684, 879, 1143
DinI GGCGCC 1 cut(s) 48
EaeI YGGCCR 2 cut(s) 132, 151
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 3 cut(s) 329, 739, 902
EarI CTCTTC 3 cut(s) 329, 739, 902
EciI GGCGGA 2 cut(s) 38, 402
Ecl136II GAGCTC 1 cut(s) 457
EclXI CGGCCG 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 64
Eco52I CGGCCG 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 457
Eco57I CTGAAG 3 cut(s) 720, 963, 1096
Eco72I CACGTG 1 cut(s) 829
Eco88I CYCGRG 2 cut(s) 654, 1193
Eco91I GGTNACC 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 457
EcoO65I GGTNACC 1 cut(s) 193
EcoRI GAATTC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 814
EcoT22I ATGCAT 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 459
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
Esp3I CGTCTC 1 cut(s) 266
FaeI CATG 5 cut(s) 223, 380, 463, 1079, 1136
FaqI GGGAC 1 cut(s) 599
FatI CATG 5 cut(s) 219, 376, 459, 1075, 1132
FauNDI CATATG 1 cut(s) 369
FblI GTMKAC 1 cut(s) 940
Fnu4HI GCNGC 9 cut(s) 45, 151, 359, 362, 416, 750, 884, 923, 1130
FokI GGATG 2 cut(s) 72, 960
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 9 cut(s) 45, 151, 359, 362, 416, 750, 884, 923, 1130
FspBI CTAG 3 cut(s) 597, 650, 1049
GlaI GCGC 2 cut(s) 48, 238
GluI GCNGC 9 cut(s) 45, 151, 359, 362, 416, 750, 884, 923, 1130
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 2 cut(s) 134, 153
HapII CCGG 2 cut(s) 62, 131
HgaI GACGC 1 cut(s) 349
HhaI GCGC 2 cut(s) 49, 239
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 5 cut(s) 223, 380, 463, 1079, 1136
Hin6I GCGC 2 cut(s) 47, 237
HinP1I GCGC 2 cut(s) 47, 237
HincII GTYRAC 2 cut(s) 941, 1008
HindII GTYRAC 2 cut(s) 941, 1008
HinfI GANTC 4 cut(s) 156, 394, 657, 1105
HpaII CCGG 2 cut(s) 62, 131
HphI GGTGA 3 cut(s) 187, 652, 1046
Hpy166II GTNNAC 3 cut(s) 442, 941, 1008
Hpy188III TCNNGA 2 cut(s) 377, 398
Hpy8I GTNNAC 3 cut(s) 442, 941, 1008
Hpy99I CGWCG 2 cut(s) 58, 942
HpyAV CCTTC 2 cut(s) 987, 1120
HpyCH4III ACNGT 8 cut(s) 54, 518, 710, 726, 938, 945, 1036, 1209
HpyCH4IV ACGT 4 cut(s) 183, 285, 828, 969
HpyCH4V TGCA 8 cut(s) 311, 569, 580, 749, 832, 886, 1136, 1184
HpyF10VI GCNNNNNNNGC 6 cut(s) 55, 150, 243, 252, 844, 853
HpyF3I CTNAG 6 cut(s) 140, 344, 664, 684, 879, 1143
HpySE526I ACGT 4 cut(s) 183, 285, 828, 969
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 5 cut(s) 223, 380, 463, 1079, 1136
HspAI GCGC 2 cut(s) 47, 237
KasI GGCGCC 1 cut(s) 46
KpnI GGTACC 1 cut(s) 709
LmnI GCTCC 2 cut(s) 511, 853
LpnPI CCDG 9 cut(s) 75, 89, 144, 307, 568, 712, 729, 801, 828
Lsp1109I GCAGC 6 cut(s) 345, 348, 761, 870, 934, 1141
LweI GCATC 4 cut(s) 255, 319, 841, 1033
MaeI CTAG 3 cut(s) 597, 650, 1049
MaeII ACGT 4 cut(s) 183, 285, 828, 969
MaeIII GTNAC 4 cut(s) 193, 623, 658, 694
MboII GAAGA 8 cut(s) 166, 310, 346, 421, 596, 726, 919, 1052
MfeI CAATTG 2 cut(s) 619, 984
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 4 cut(s) 285, 459, 516, 715
MluCI AATT 6 cut(s) 265, 619, 788, 887, 984, 1029
Mly113I GGCGCC 1 cut(s) 47
MlyI GAGTC 2 cut(s) 666, 1099
Mph1103I ATGCAT 1 cut(s) 313
MroXI GAANNNNTTC 2 cut(s) 311, 428
MseI TTAA 5 cut(s) 590, 629, 728, 1064, 1188
MslI CAYNNNNRTG 2 cut(s) 32, 381
MspI CCGG 2 cut(s) 62, 131
MspR9I CCNGG 1 cut(s) 816
MunI CAATTG 2 cut(s) 619, 984
Mva1269I GAATGC 3 cut(s) 126, 311, 313
MvaI CCWGG 1 cut(s) 816
MwoI GCNNNNNNNGC 6 cut(s) 55, 150, 243, 252, 844, 853
NarI GGCGCC 1 cut(s) 47
NdeI CATATG 1 cut(s) 369
NlaIII CATG 5 cut(s) 223, 380, 463, 1079, 1136
NlaIV GGNNCC 4 cut(s) 48, 66, 707, 865
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 3 cut(s) 193, 623, 658
NsiI ATGCAT 1 cut(s) 313
NspI RCATGY 1 cut(s) 1136
PaeI GCATGC 1 cut(s) 1136
PaeR7I CTCGAG 1 cut(s) 654
PagI TCATGA 1 cut(s) 376
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 3 cut(s) 126, 311, 313
PdmI GAANNNNTTC 2 cut(s) 311, 428
PfeI GAWTC 2 cut(s) 156, 394
PkrI GCNGC 9 cut(s) 46, 152, 360, 363, 417, 751, 885, 924, 1131
Ple19I CGATCG 1 cut(s) 20
PleI GAGTC 2 cut(s) 665, 1099
PluTI GGCGCC 1 cut(s) 50
PmaCI CACGTG 1 cut(s) 829
PmlI CACGTG 1 cut(s) 829
PpsI GAGTC 2 cut(s) 665, 1099
Ppu21I YACGTR 1 cut(s) 829
Psp124BI GAGCTC 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 814
PspCI CACGTG 1 cut(s) 829
PspEI GGTNACC 1 cut(s) 193
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 4 cut(s) 48, 66, 707, 865
PspPI GGNCC 1 cut(s) 64
PspXI VCTCGAGB 1 cut(s) 654
PstI CTGCAG 1 cut(s) 751
PstNI CAGNNNCTG 2 cut(s) 671, 878
PsuI RGATCY 1 cut(s) 537
PvuI CGATCG 1 cut(s) 20
RsaI GTAC 5 cut(s) 167, 206, 443, 707, 1199
RsaNI GTAC 5 cut(s) 166, 205, 442, 706, 1198
RseI CAYNNNNRTG 2 cut(s) 32, 381
SacI GAGCTC 1 cut(s) 459
SalI GTCGAC 1 cut(s) 939
SaqAI TTAA 5 cut(s) 590, 629, 728, 1064, 1188
SatI GCNGC 9 cut(s) 45, 151, 359, 362, 416, 750, 884, 923, 1130
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 2 cut(s) 666, 1099
ScrFI CCNGG 1 cut(s) 816
SduI GDGCHC 4 cut(s) 285, 459, 516, 715
SfaNI GCATC 4 cut(s) 255, 319, 841, 1033
SfcI CTRYAG 1 cut(s) 747
SfoI GGCGCC 1 cut(s) 48
Sfr274I CTCGAG 1 cut(s) 654
SinI GGWCC 1 cut(s) 64
SlaI CTCGAG 1 cut(s) 654
SmiMI CAYNNNNRTG 2 cut(s) 32, 381
SmlI CTYRAG 1 cut(s) 654
SmoI CTYRAG 1 cut(s) 654
SphI GCATGC 1 cut(s) 1136
Sse9I AATT 6 cut(s) 265, 619, 788, 887, 984, 1029
SsiI CCGC 8 cut(s) 23, 44, 95, 150, 198, 290, 413, 416
SspDI GGCGCC 1 cut(s) 46
SspMI CTAG 3 cut(s) 597, 650, 1049
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 1 cut(s) 814
TaaI ACNGT 8 cut(s) 54, 518, 710, 726, 938, 945, 1036, 1209
TaiI ACGT 4 cut(s) 186, 288, 831, 972
TaqI TCGA 6 cut(s) 315, 327, 487, 655, 940, 1119
TasI AATT 6 cut(s) 265, 619, 788, 887, 984, 1029
TatI WGTACW 3 cut(s) 165, 204, 441
TauI GCSGC 3 cut(s) 47, 153, 418
TfiI GAWTC 2 cut(s) 156, 394
Tru1I TTAA 5 cut(s) 590, 629, 728, 1064, 1188
Tru9I TTAA 5 cut(s) 590, 629, 728, 1064, 1188
TscAI CASTG 4 cut(s) 472, 521, 751, 1090
TseFI GTSAC 3 cut(s) 193, 623, 658
TseI GCWGC 6 cut(s) 358, 361, 749, 883, 922, 1129
Tsp45I GTSAC 3 cut(s) 193, 623, 658
TspDTI ATGAA 3 cut(s) 148, 912, 1169
TspRI CASTG 4 cut(s) 472, 521, 751, 1090
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 1 cut(s) 265
XceI RCATGY 1 cut(s) 1136
XhoI CTCGAG 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 940
XmnI GAANNNNTTC 2 cut(s) 311, 428
XspI CTAG 3 cut(s) 597, 650, 1049
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.