MD15G1147300.v1.1

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012722
Physical Location & Seq
Reverse (-)
10938594 .. 10942321
3728 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1147300.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGAGGGAGGAGGGCCCGCAGCTCAATCGGCGGACACTGTGATGTCGGAGGCGGCGCCACCGTCGCATCCGGACCCCAACCACCCACAGCAACATCCGCCACCGCCGCATCAGGCCATGGGGGGAGTGGAGAGCATTCCGGCCACGCTCAGCCACGGCGGCCGATTCATTCAGTACAACATCTTCGGCAACGTCTTCGAGGTCACCGCCAAGTACAAGCCCCCCATCATGCCCATCGGTAAAGGCGCATACGGCATCGTTTGCTCGGCTTTGAATTCGGAGACGAACGAGCACGTTGCGATAAAGAAGATTGCGAATGCGTTCGACAACAAGATCGATGCGAAGAGGACGCTGAGGGAGATCAAACTGCTGCGGCATATGGATCATGAGAATGTTGTTGCGATCCGGGATATAATCCCTCCACCGCAGAGGAACTCCTTCAATGATGTTTACATTGCGTATGAGCTCATGGACACCGACCTCCACCAAATCATTCGATCCAATCAAGCACTCTCCGAGGAGCACTGTCAGTATTTCTTATATCAGATCCTCCGAGGATTGAAATACATACACTCTGCAAATGTTCTGCACAGGGACTTAAAACCTAGCAATCTTCTATTAAATGCCAATTGTGACTTAAAAATATGTGATTTTGGACTAGCTCGAGTCACCTCTGAGACTGATTTTATGACTGAGTATGTTGTTACAAGATGGTACCGTGCCCCAGAGCTATTGTTAAACTCTTCAGATTACACTGCAGCTATTGATGTATGGTCAGTAGGTTGTATTTTTATGGAATTGATGGATCGGAAGCCATTATTTCCTGGCAGAGATCACGTGCATCAGCTTCGTCTGCTTTTGGAGCTGATTGGCACCCCATCAGAGGCTGAGCTGCAATTTCTAAATGAAAATGCTAAGAGATACATTCGGCAGCTTCCTCTCTACCGTCGACAGTCATTTACCGAGAAGTTTCCTCACGTCCATCCTTCAGCAATTGATCTAGTTGAAAAGATGTTGACATTTGATCCTACTCAGAGAATTACTGTTGAAGATGCCCTAGCTCACCCCTATTTAACATCTCTCCATGACATCAGTGATGAGCCTGTTTGCACGACTCCCTTCAGCTTCGACTTTGAGCAGCATGCACTCTCTGAGGAACAGATGAAAGAGCTGATCTACCGAGAGGCACTTGCATTTAACCCCGAGTATCAGCTACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000302 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005802 GO:0005856 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009314 GO:0009403 GO:0009404 GO:0009409 GO:0009411 GO:0009416 GO:0009524 GO:0009555 GO:0009574 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009682 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009791 GO:0009856 GO:0009864 GO:0009867 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010150 GO:0010183 GO:0010224 GO:0010229 GO:0010468 GO:0012505 GO:0015630 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0022414 GO:0022622 GO:0023014 GO:0023052 GO:0031347 GO:0031349 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033993 GO:0034641 GO:0035556 GO:0035670 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042430 GO:0042435 GO:0042493 GO:0042542 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0044706 GO:0045087 GO:0045088 GO:0045089 GO:0046217 GO:0046483 GO:0046677 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0050918 GO:0051301 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0080134 GO:0080135 GO:0080136 GO:0090567 GO:0090693 GO:0097305 GO:0098542 GO:0098791 GO:0099402 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Pkinase

Protein Analysis

407

Amino Acids

46.27

Weight (kDa)

5.66

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 78 - 358 3.1e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 79 - 269 4.6e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012722)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43790
fragaria_vesca FvH4_1g00480 FvH4_1g00480
malus_domestica MD02G1004000.v1.1 MD15G1147300.v1.1
prunus_persica Prupe.7G267300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084991
rosa_laevigata RLG00000015654
rosa_multiflora Rmu_co8283871.1_g000001 Rmu_sc0001196.1_g000005 Rmu_sc0008647.1_g000018
rosa_roxburghii Rroxscaffold_2G00155650
rosa_rugosa Rorug01G0457600
rosa_samantha Rh2AG005500 Rh2BG006500 Rh2CG007000 Rh2DG006900
rosa_wichuraiana Rw2G000520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 714
AccB1I GGYRCC 3 cut(s) 55, 714, 872
AccI GTMKAC 1 cut(s) 949
AccIII TCCGGA 1 cut(s) 70
AclWI GGATC 6 cut(s) 390, 397, 492, 541, 813, 1019
AcoI YGGCCR 2 cut(s) 141, 160
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 3 cut(s) 729, 972, 1105
AcvI CACGTG 1 cut(s) 838
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 3 cut(s) 176, 215, 716
AfiI CCNNNNNNNGG 1 cut(s) 883
AgsI TTSAA 5 cut(s) 274, 442, 562, 1007, 1049
AjiI CACGTC 1 cut(s) 979
AjnI CCWGG 1 cut(s) 823
Alw21I GWGCWC 3 cut(s) 294, 468, 525
Alw26I GTCTC 2 cut(s) 275, 671
AlwI GGATC 6 cut(s) 390, 397, 492, 541, 813, 1019
AlwNI CAGNNNCTG 1 cut(s) 887
Ama87I CYCGRG 2 cut(s) 663, 1202
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 4 cut(s) 14, 114, 141, 160
ApaI GGGCCC 1 cut(s) 18
ApeKI GCWGC 6 cut(s) 20, 370, 758, 892, 931, 1138
ApoI RAATTY 1 cut(s) 274
Asp700I GAANNNNTTC 2 cut(s) 320, 437
Asp718I GGTACC 1 cut(s) 714
AspLEI GCGC 2 cut(s) 58, 248
AspS9I GGNCC 3 cut(s) 14, 15, 73
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 3 cut(s) 196, 661, 1055
AvaI CYCGRG 2 cut(s) 663, 1202
AvaII GGWCC 1 cut(s) 73
BaeGI GKGCMC 2 cut(s) 18, 724
BanI GGYRCC 3 cut(s) 55, 714, 872
BanII GRGCYC 2 cut(s) 18, 468
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbrPI CACGTG 1 cut(s) 838
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 3 cut(s) 294, 468, 525
BbvCI CCTCAGC 1 cut(s) 353
BbvI GCAGC 6 cut(s) 32, 357, 770, 879, 943, 1150
BccI CCATC 6 cut(s) 233, 242, 705, 796, 886, 990
BceAI ACGGC 2 cut(s) 172, 268
BcgI CGANNNNNNTGC 8 cut(s) 9, 43, 178, 212, 278, 312, 830, 864
BciT130I CCWGG 1 cut(s) 825
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 2 cut(s) 275, 671
BfaI CTAG 4 cut(s) 606, 659, 1001, 1058
BfmI CTRYAG 2 cut(s) 756, 1214
BfoI RGCGCY 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 159
BlpI GCTNAGC 2 cut(s) 149, 888
Bme1390I CCNGG 2 cut(s) 407, 825
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 2 cut(s) 663, 1202
BmgBI CACGTC 1 cut(s) 979
BmgT120I GGNCC 3 cut(s) 14, 15, 73
BmiI GGNNCC 5 cut(s) 16, 57, 75, 716, 874
BmrFI CCNGG 2 cut(s) 407, 825
BmsI GCATC 6 cut(s) 76, 118, 264, 328, 850, 1042
BpiI GAAGAC 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 353
Bpu1102I GCTNAGC 2 cut(s) 149, 888
BpuMI CCSGG 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 838
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 4 cut(s) 117, 154, 516, 553
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 883
Bse3DI GCAATG 1 cut(s) 453
BseAI TCCGGA 1 cut(s) 70
BseBI CCWGG 1 cut(s) 825
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 117, 154, 516, 553
BseGI GGATG 3 cut(s) 67, 94, 982
BseLI CCNNNNNNNGG 1 cut(s) 883
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 7 cut(s) 163, 344, 666, 684, 879, 1046, 1143
BseRI GAGGAG 2 cut(s) 24, 533
BseSI GKGCMC 2 cut(s) 18, 724
BseX3I CGGCCG 1 cut(s) 160
BseXI GCAGC 6 cut(s) 32, 357, 770, 879, 943, 1150
BsgI GTGCAG 1 cut(s) 572
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 4 cut(s) 16, 116, 143, 162
BshNI GGYRCC 3 cut(s) 55, 714, 872
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 3 cut(s) 294, 468, 525
BsiHKCI CYCGRG 2 cut(s) 663, 1202
BsiSI CCGG 3 cut(s) 71, 140, 406
BslFI GGGAC 1 cut(s) 608
BslI CCNNNNNNNGG 1 cut(s) 883
BsmAI GTCTC 2 cut(s) 275, 671
BsmBI CGTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 608
BsmI GAATGC 2 cut(s) 135, 322
BsnI GGCC 4 cut(s) 16, 116, 143, 162
BsoBI CYCGRG 2 cut(s) 663, 1202
Bsp120I GGGCCC 1 cut(s) 14
Bsp1286I GDGCHC 5 cut(s) 18, 294, 468, 525, 724
Bsp13I TCCGGA 1 cut(s) 70
Bsp1720I GCTNAGC 2 cut(s) 149, 888
Bsp19I CCATGG 1 cut(s) 117
BspANI GGCC 4 cut(s) 16, 116, 143, 162
BspCNI CTCAG 7 cut(s) 162, 345, 667, 685, 880, 1045, 1144
BspDI ATCGAT 1 cut(s) 336
BspEI TCCGGA 1 cut(s) 70
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 5 cut(s) 16, 57, 75, 716, 874
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 6 cut(s) 390, 397, 492, 541, 813, 1019
BspT107I GGYRCC 3 cut(s) 55, 714, 872
BsrDI GCAATG 1 cut(s) 453
BssECI CCNNGG 4 cut(s) 117, 154, 516, 553
BssNI GRCGYC 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 825
Bst4CI ACNGT 8 cut(s) 40, 63, 527, 719, 947, 954, 1045, 1218
Bst6I CTCTTC 2 cut(s) 338, 748
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 838
BstC8I GCNNGC 2 cut(s) 18, 1143
BstDEI CTNAG 8 cut(s) 149, 353, 675, 693, 888, 915, 1032, 1152
BstDSI CCRYGG 2 cut(s) 117, 154
BstEII GGTNACC 1 cut(s) 202
BstF5I GGATG 3 cut(s) 67, 94, 982
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 2 cut(s) 58, 248
BstMAI GTCTC 2 cut(s) 275, 671
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 9 cut(s) 29, 64, 97, 106, 159, 252, 261, 853, 862
BstNI CCWGG 1 cut(s) 825
BstNSI RCATGY 1 cut(s) 1145
BstPI GGTNACC 1 cut(s) 202
BstSCI CCNGG 2 cut(s) 405, 823
BstSFI CTRYAG 2 cut(s) 756, 1214
BstSLI GKGCMC 2 cut(s) 18, 724
BstV1I GCAGC 6 cut(s) 32, 357, 770, 879, 943, 1150
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
BstZI CGGCCG 1 cut(s) 160
Bsu15I ATCGAT 1 cut(s) 336
BsuRI GGCC 4 cut(s) 16, 116, 143, 162
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 2 cut(s) 117, 154
BtrI CACGTC 1 cut(s) 979
BtsCI GGATG 3 cut(s) 67, 94, 982
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 4 cut(s) 36, 523, 753, 1099
Cac8I GCNNGC 2 cut(s) 18, 1143
CaiI CAGNNNCTG 1 cut(s) 887
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 58, 248
Cfr13I GGNCC 3 cut(s) 14, 15, 73
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 1 cut(s) 358
Csp6I GTAC 3 cut(s) 175, 214, 715
CviAII CATG 6 cut(s) 118, 229, 386, 469, 1085, 1142
CviQI GTAC 3 cut(s) 175, 214, 715
DdeI CTNAG 8 cut(s) 149, 353, 675, 693, 888, 915, 1032, 1152
DinI GGCGCC 1 cut(s) 57
EaeI YGGCCR 2 cut(s) 141, 160
EagI CGGCCG 1 cut(s) 160
Eam1104I CTCTTC 2 cut(s) 338, 748
EarI CTCTTC 2 cut(s) 338, 748
EciI GGCGGA 2 cut(s) 47, 87
Ecl136II GAGCTC 1 cut(s) 466
EclXI CGGCCG 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 2 cut(s) 18, 468
Eco47I GGWCC 1 cut(s) 73
Eco52I CGGCCG 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 3 cut(s) 729, 972, 1105
Eco72I CACGTG 1 cut(s) 838
Eco88I CYCGRG 2 cut(s) 663, 1202
Eco91I GGTNACC 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 14
EcoO65I GGTNACC 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 2 cut(s) 18, 468
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 6 cut(s) 121, 232, 389, 472, 1088, 1145
FaqI GGGAC 1 cut(s) 608
FatI CATG 6 cut(s) 117, 228, 385, 468, 1084, 1141
FauI CCCGC 1 cut(s) 25
FauNDI CATATG 1 cut(s) 378
FblI GTMKAC 1 cut(s) 949
FokI GGATG 3 cut(s) 54, 81, 969
FriOI GRGCYC 2 cut(s) 18, 468
FspBI CTAG 4 cut(s) 606, 659, 1001, 1058
GlaI GCGC 2 cut(s) 57, 247
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 4 cut(s) 16, 116, 143, 162
HapII CCGG 3 cut(s) 71, 140, 406
HgaI GACGC 1 cut(s) 358
HhaI GCGC 2 cut(s) 58, 248
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 6 cut(s) 121, 232, 389, 472, 1088, 1145
Hin6I GCGC 2 cut(s) 56, 246
HinP1I GCGC 2 cut(s) 56, 246
HincII GTYRAC 2 cut(s) 950, 1017
HindII GTYRAC 2 cut(s) 950, 1017
HinfI GANTC 3 cut(s) 165, 666, 1114
HpaII CCGG 3 cut(s) 71, 140, 406
HphI GGTGA 3 cut(s) 196, 661, 1055
Hpy166II GTNNAC 3 cut(s) 451, 950, 1017
Hpy188III TCNNGA 2 cut(s) 71, 386
Hpy8I GTNNAC 3 cut(s) 451, 950, 1017
Hpy99I CGWCG 2 cut(s) 67, 951
HpyAV CCTTC 3 cut(s) 448, 996, 1129
HpyCH4III ACNGT 8 cut(s) 40, 63, 527, 719, 947, 954, 1045, 1218
HpyCH4IV ACGT 4 cut(s) 192, 294, 837, 978
HpyCH4V TGCA 8 cut(s) 578, 589, 758, 841, 895, 1110, 1145, 1193
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 64, 97, 106, 159, 252, 261, 853, 862
HpyF3I CTNAG 8 cut(s) 149, 353, 675, 693, 888, 915, 1032, 1152
HpySE526I ACGT 4 cut(s) 192, 294, 837, 978
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 6 cut(s) 121, 232, 389, 472, 1088, 1145
HspAI GCGC 2 cut(s) 56, 246
KasI GGCGCC 1 cut(s) 55
Kpn2I TCCGGA 1 cut(s) 70
KpnI GGTACC 1 cut(s) 718
LmnI GCTCC 2 cut(s) 520, 862
LpnPI CCDG 9 cut(s) 84, 98, 153, 419, 577, 738, 810, 837, 1116
Lsp1109I GCAGC 6 cut(s) 32, 357, 770, 879, 943, 1150
LweI GCATC 6 cut(s) 76, 118, 264, 328, 850, 1042
MaeI CTAG 4 cut(s) 606, 659, 1001, 1058
MaeII ACGT 4 cut(s) 192, 294, 837, 978
MaeIII GTNAC 4 cut(s) 202, 632, 667, 703
MboII GAAGA 7 cut(s) 175, 187, 319, 355, 605, 735, 1061
MfeI CAATTG 2 cut(s) 628, 993
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 5 cut(s) 18, 294, 468, 525, 724
MluCI AATT 6 cut(s) 274, 628, 797, 896, 993, 1038
Mly113I GGCGCC 1 cut(s) 56
MlyI GAGTC 2 cut(s) 675, 1108
MmeI TCCRAC 1 cut(s) 27
MroI TCCGGA 1 cut(s) 70
MroXI GAANNNNTTC 2 cut(s) 320, 437
MseI TTAA 6 cut(s) 599, 620, 638, 737, 1073, 1197
MslI CAYNNNNRTG 2 cut(s) 41, 390
MspI CCGG 3 cut(s) 71, 140, 406
MspR9I CCNGG 2 cut(s) 407, 825
MunI CAATTG 2 cut(s) 628, 993
Mva1269I GAATGC 2 cut(s) 135, 322
MvaI CCWGG 1 cut(s) 825
MwoI GCNNNNNNNGC 9 cut(s) 29, 64, 97, 106, 159, 252, 261, 853, 862
NarI GGCGCC 1 cut(s) 56
NciI CCSGG 1 cut(s) 407
NcoI CCATGG 1 cut(s) 117
NdeI CATATG 1 cut(s) 378
NlaIII CATG 6 cut(s) 121, 232, 389, 472, 1088, 1145
NlaIV GGNNCC 5 cut(s) 16, 57, 75, 716, 874
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 3 cut(s) 202, 632, 667
NspI RCATGY 1 cut(s) 1145
PaeI GCATGC 1 cut(s) 1145
PaeR7I CTCGAG 1 cut(s) 663
PagI TCATGA 1 cut(s) 385
PctI GAATGC 2 cut(s) 135, 322
PdmI GAANNNNTTC 2 cut(s) 320, 437
PfeI GAWTC 1 cut(s) 165
PfoI TCCNGGA 1 cut(s) 405
PleI GAGTC 2 cut(s) 674, 1108
PluTI GGCGCC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 838
PmlI CACGTG 1 cut(s) 838
PpsI GAGTC 2 cut(s) 674, 1108
Ppu21I YACGTR 1 cut(s) 838
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 823
PspCI CACGTG 1 cut(s) 838
PspEI GGTNACC 1 cut(s) 202
PspGI CCWGG 1 cut(s) 823
PspN4I GGNNCC 5 cut(s) 16, 57, 75, 716, 874
PspOMI GGGCCC 1 cut(s) 14
PspPI GGNCC 3 cut(s) 14, 15, 73
PspXI VCTCGAGB 1 cut(s) 663
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 1 cut(s) 546
RsaI GTAC 3 cut(s) 176, 215, 716
RsaNI GTAC 3 cut(s) 175, 214, 715
RseI CAYNNNNRTG 2 cut(s) 41, 390
SacI GAGCTC 1 cut(s) 468
SalI GTCGAC 1 cut(s) 948
SaqAI TTAA 6 cut(s) 599, 620, 638, 737, 1073, 1197
Sau96I GGNCC 3 cut(s) 14, 15, 73
SchI GAGTC 2 cut(s) 675, 1108
ScrFI CCNGG 2 cut(s) 407, 825
SduI GDGCHC 5 cut(s) 18, 294, 468, 525, 724
SfaNI GCATC 6 cut(s) 76, 118, 264, 328, 850, 1042
SfcI CTRYAG 2 cut(s) 756, 1214
SfoI GGCGCC 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 73
SlaI CTCGAG 1 cut(s) 663
SmiMI CAYNNNNRTG 2 cut(s) 41, 390
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
SphI GCATGC 1 cut(s) 1145
Sse9I AATT 6 cut(s) 274, 628, 797, 896, 993, 1038
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 4 cut(s) 606, 659, 1001, 1058
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 405, 823
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 8 cut(s) 40, 63, 527, 719, 947, 954, 1045, 1218
TaiI ACGT 4 cut(s) 195, 297, 840, 981
TaqI TCGA 7 cut(s) 198, 324, 336, 496, 664, 949, 1128
TasI AATT 6 cut(s) 274, 628, 797, 896, 993, 1038
TatI WGTACW 2 cut(s) 174, 213
TauI GCSGC 4 cut(s) 56, 109, 162, 376
TfiI GAWTC 1 cut(s) 165
Tru1I TTAA 6 cut(s) 599, 620, 638, 737, 1073, 1197
Tru9I TTAA 6 cut(s) 599, 620, 638, 737, 1073, 1197
TscAI CASTG 4 cut(s) 43, 530, 760, 1099
TseFI GTSAC 3 cut(s) 202, 632, 667
TseI GCWGC 6 cut(s) 20, 370, 758, 892, 931, 1138
Tsp45I GTSAC 3 cut(s) 202, 632, 667
TspDTI ATGAA 3 cut(s) 157, 921, 1178
TspRI CASTG 4 cut(s) 43, 530, 760, 1099
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 1 cut(s) 274
XceI RCATGY 1 cut(s) 1145
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XhoI CTCGAG 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 949
XmnI GAANNNNTTC 2 cut(s) 320, 437
XspI CTAG 4 cut(s) 606, 659, 1001, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.