Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009118

Physical Location & Seq

Genomic Coordinates Reverse (-)

4855219 .. 4862209

Gene Structure 6991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1060500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGGCGGCATCGGAGACCTCCAACGGAACGACCTTGAGGCACGCTTTTGGAAATGTCCTCTCCTTCTTCATCCTCATCCTGATCGGCGTCCTCGCATTTTCGATCCGGCTCTTCTCTGTTATAAAGTACGAGAGTGTTATTCACGAGTTTGATCCGTACTTCAATTACAGAGTCACTCAGTTTCTGACGAAGAATGGAATATACGATTTTTGGAACTGGTTTGATGACCGAACCTGGTATCCTCTTGGTCGTGTGATTGGTGGAACTGTTTACCCTGGATTGACCTTGACAGCAGGATCCCTATGGTGGTTTTTGAATTCATTGAACATTCCTCTTTCCGTGGAAACTATCTGTGTATTTACTGCCCCTATATTCTCTGCTTTCACATCGTGGGCAACTTACCTGCTGACTCGGGAAGTTAAGGGTGCTGGTGCTGGACTAACAGCTGCTGCTCTTATAGCCATGGTCCCGTCATATATTTCTCGGTCAGTGGCTGGAAGCTATGACAATGAAGCTGTAGCTATTTTTGCTTTGATCTTCACTTTCTATCTTTATATAAAGACTCTGAATACAGGGTCCCTCTTCTATGCAACTCTTAATGCCATAGCGTACTTTTACATGGTTTGCTCTTGGGGAGGCTACACATTTATTATTAACCTTATTCCAATGCATGTGCTTCTTTGCATTGTAACTGGCCGCTACTCTTCTCGTCTATACATTGCATATGCTCCTCTTGTTGTTTTGGGAACGTTGTTAGCGGCTTTGGTGCCGGTGGTTGGATTCAATGCAGTCATGACATCAGAACATTTTGCTTCCTTCTTGGTTTTTATTATTTTACATGTGGTCGCTCTTGTGTACTATATCAAAGGGATTCTCTCTCCGAAAATGTTCAAAGTGGCTGTCACGCTTGTAGTATCCATTGGCCTGCTAGTATGTTTAGCTGTGGTAGCAGTACTCGTAGCTCTGGTAGCTTCAAGCCCAACAAAGGGATGGAGTGGGCGAAGTCTAAGTCTACTTGATCCAACTTATGCGAGCAAGTACATACCAATTATTGCTAGTGTTAGTGAGCACCAGCCTCCTACTTGGCCCTCTTACTTTATGGATATTAATGTGTTGGCCTTCTTGGTTCCAGCTGGAATTATTGCATGCTTTTCACCCTTATCAGATGCAAGCTCTTTTGTGATCCTCTATATTGTAACATCAGTATATTTCTCTGGAGTCATGGTGCGACTTATGCTAGTACTTGCTCCAGCAGCGTGCATCATGTCTGGGATTGCTCTGTCTGAAGCTTTTGACGTTTTCACACGATCAATCAAGTTTCAGTTACCCGGTCTTCTAGTGCCTTCCCAAGTTGATGCAGGGGAAACCAGTTCCGAGAACACTGTATCCCAGAATGAAGTGCCGAAAACAGATAAAAGAGCAGACACATTAAAGGAGAGGCCCTCGAGAAAGAACAGGAAGAAGGAAAAGGAACCAGTTGAAAAGCCTTCCATTAAATCTCAAATTGAAAAGAGGCTTCTGGTTTTACCATTTGAGGGATCTATTCTTGCTATATTTCTACTTGTGTTGCTTGGAGCCTTCTATGTGGTTCATTGTGTCTGGGCAGCAGCAGAAGCTTATTCAGCCCCGTCTATTGTTCTAACATCTCATTCACATGATGGTCTTCATGTTTTTGATGATTTTAGAGAGGCTTATGCGTGGCTAAGCCACAACACTGAGGTGGATGATAAAGTTGCCTCTTGGTGGGATTATGGGTACCAAACTACTGCCATGGCTAATCGCACTGTCATTGTTGACAATAATACATGGAACAACACGCATATTGCAACCGTGGGTACTGCCATGTCTTCTCCAGAAAAGGAAGCTTGGGAAATTTTCAACTCGTTGGATGTAAAATATGTCCTCGTTGTTTTTGGAGGTCTTGTTGGCTACCCCAGTGATGATATTAACAAGTTCTTGTGGATGGTCCGAATTGGAGGGGGCGTATTCCCTCATATCAAGGAACCTGATTACTTAAGGGATGGCCAGTATCGCATTGACAATCAGGCGACCCCCACCATGCTAAATTGTCTCATGTACAAGCTCTCTTATTACAGGTTTGTGGACACAGACGGTAAAGGCTTTGATAGGGTGAGGCAAACAGAAATCGGAAAGAAACATTTCAAACTTACTCATTTTGAAGAGGTGTTCACAACTCACCATTGGATGGTTCGGTTATACAAACTAAAACCACCAAAGAACAGGATCCGAGGAAAGACAAAGAAGTCAAAATCGAAATCGAGCTCAACGAGTAGCACAAAGAAGAGCGGAACGAAAAGGAATCCTTGGCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004576 GO:0004579 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008250 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016740 GO:0016757 GO:0016758 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0031984 GO:0032991 GO:0034645 GO:0036211 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043687 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0047484 GO:0048583 GO:0050789 GO:0050896 GO:0065007 GO:0070085 GO:0071704 GO:0071944 GO:0080134 GO:0098796 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00510 ko00513 ko01100 ko04141 map00510 map00513 map01100 map04141

Pfam Domains

Protein Families

STT3

Protein Analysis

778

Amino Acids

86.79

Weight (kDa)

9.09

Isoelectric Point (pI)

34.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
STT3	PF02516	19 - 422	3.5e-104	Oligosaccharyl transferase STT3, N-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009118)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19690 AT5G19690
fragaria_vesca	FvH4_1g05850 FvH4_1g05850
malus_domestica	MD02G1060500.v1.1 MD15G1194000.v1.1
prunus_persica	Prupe.7G222300_v2.0.a1 Prupe.7G222300_v2.0.a1
pyrus_communis	pycom02g04970 pycom15g17380
rosa_chinensis	RchiOBHm_Chr2g0091511 RchiOBHm_Chr2g0091521 RchiOBHm_Chr2g0091601
rosa_laevigata	RLG00000016232
rosa_multiflora	Rmu_sc0002407.1_g000006
rosa_roxburghii	Rroxscaffold_2G00149970
rosa_rugosa	Rorug02G0018400
rosa_samantha	Rh2AG063600 Rh2BG063000 Rh2CG064700 Rh2DG063100
rosa_wichuraiana	Rw2G005680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	122
AasI	GACNNNNNNGTC	1 cut(s)	2263
Acc36I	ACCTGC	1 cut(s)	411
Acc65I	GGTACC	1 cut(s)	1755
AccB1I	GGYRCC	2 cut(s)	766, 1755
AccB7I	CCANNNNNTGG	1 cut(s)	2206
AccBSI	CCGCTC	1 cut(s)	2307
AccI	GTMKAC	1 cut(s)	1012
AciI	CCGC	4 cut(s)	5, 697, 758, 2307
AclI	AACGTT	1 cut(s)	749
AclWI	GGATC	9 cut(s)	97, 146, 291, 304, 1013, 1177, 1546, 2239, 2252
AcoI	YGGCCR	2 cut(s)	694, 2023
AcsI	RAATTY	2 cut(s)	316, 1872
AcuI	CTGAAG	1 cut(s)	1305
AcyI	GRCGYC	1 cut(s)	87
AdeI	CACNNNGTG	1 cut(s)	390
AfiI	CCNNNNNNNGG	7 cut(s)	306, 776, 985, 986, 1535, 1743, 2206
AflII	CTTAAG	1 cut(s)	2014
AflIII	ACRYGT	1 cut(s)	838
AjnI	CCWGG	2 cut(s)	233, 274
AleI	CACNNNNGTG	1 cut(s)	1718
AloI	GAACNNNNNNTCC	4 cut(s)	223, 255, 1370, 1402
Alw21I	GWGCWC	2 cut(s)	1071, 2285
Alw26I	GTCTC	2 cut(s)	8, 2075
AlwI	GGATC	9 cut(s)	97, 146, 291, 304, 1013, 1177, 1546, 2239, 2252
AlwNI	CAGNNNCTG	3 cut(s)	184, 449, 494
Ama87I	CYCGRG	2 cut(s)	411, 1444
AoxI	GGCC	6 cut(s)	694, 922, 1085, 1116, 1439, 2023
ApeKI	GCWGC	5 cut(s)	446, 449, 1253, 1604, 1607
ApoI	RAATTY	2 cut(s)	316, 1872
ArsI	GACNNNNNNTTYG	4 cut(s)	885, 917, 994, 1026
AseI	ATTAAT	1 cut(s)	1107
Asp700I	GAANNNNTTC	2 cut(s)	887, 2159
Asp718I	GGTACC	1 cut(s)	1755
AspS9I	GGNCC	5 cut(s)	466, 576, 1086, 1440, 1966
AsuC2I	CCSGG	1 cut(s)	1329
AsuHPI	GGTGA	3 cut(s)	1146, 2143, 2189
AvaI	CYCGRG	2 cut(s)	411, 1444
AvaII	GGWCC	3 cut(s)	466, 576, 1966
BalI	TGGCCA	1 cut(s)	2025
BamHI	GGATCC	2 cut(s)	296, 2244
BanI	GGYRCC	2 cut(s)	766, 1755
BanII	GRGCYC	1 cut(s)	2285
BauI	CACGAG	1 cut(s)	143
BbsI	GAAGAC	3 cut(s)	1325, 1655, 1839
Bbv12I	GWGCWC	2 cut(s)	1071, 2285
BbvI	GCAGC	5 cut(s)	433, 436, 1265, 1616, 1619
BccI	CCATC	5 cut(s)	984, 1652, 1957, 2015, 2200
BciT130I	CCWGG	2 cut(s)	235, 276
BciVI	GTATCC	3 cut(s)	249, 925, 1396
BcnI	CCSGG	1 cut(s)	1329
BcoDI	GTCTC	2 cut(s)	8, 2075
BfaI	CTAG	4 cut(s)	929, 1056, 1238, 1337
BfmI	CTRYAG	1 cut(s)	516
BfrI	CTTAAG	1 cut(s)	2014
BfuAI	ACCTGC	1 cut(s)	411
BfuI	GTATCC	3 cut(s)	249, 925, 1396
BisI	GCNGC	8 cut(s)	6, 447, 450, 697, 759, 1254, 1605, 1608
BlpI	GCTNAGC	1 cut(s)	1703
BlsI	GCNGC	8 cut(s)	7, 448, 451, 698, 760, 1255, 1606, 1609
BmcAI	AGTACT	2 cut(s)	954, 1242
Bme1390I	CCNGG	3 cut(s)	235, 276, 1329
Bme18I	GGWCC	3 cut(s)	466, 576, 1966
BmeT110I	CYCGRG	2 cut(s)	411, 1444
BmgT120I	GGNCC	5 cut(s)	466, 576, 1086, 1440, 1966
BmrFI	CCNGG	3 cut(s)	235, 276, 1329
BmrI	ACTGGG	1 cut(s)	1929
BmsI	GCATC	4 cut(s)	17, 1156, 1269, 1345
BmuI	ACTGGG	1 cut(s)	1929
BpiI	GAAGAC	3 cut(s)	1325, 1655, 1839
BplI	GAGNNNNNCTC	2 cut(s)	1427, 1459
BpmI	CTGGAG	3 cut(s)	1233, 1236, 1836
Bpu1102I	GCTNAGC	1 cut(s)	1703
BpuEI	CTTGAG	1 cut(s)	55
BpuMI	CCSGG	1 cut(s)	1329
BsaHI	GRCGYC	1 cut(s)	87
BsaI	GGTCTC	1 cut(s)	8
BsaJI	CCNNGG	7 cut(s)	274, 339, 462, 1770, 1830, 2248, 2324
BsaXI	ACNNNNNCTCC	6 cut(s)	1209, 1239, 1908, 1938, 1968, 1998
Bsc4I	CCNNNNNNNGG	7 cut(s)	306, 776, 985, 986, 1535, 1743, 2206
Bse118I	RCCGGY	1 cut(s)	769
Bse1I	ACTGG	6 cut(s)	221, 697, 1368, 1475, 1935, 2026
Bse3DI	GCAATG	1 cut(s)	717
BseBI	CCWGG	2 cut(s)	235, 276
BseDI	CCNNGG	7 cut(s)	274, 339, 462, 1770, 1830, 2248, 2324
BseGI	GGATG	8 cut(s)	69, 75, 995, 1729, 1894, 1968, 2026, 2211
BseLI	CCNNNNNNNGG	7 cut(s)	306, 776, 985, 986, 1535, 1743, 2206
BseMI	GCAATG	1 cut(s)	717
BseMII	CTCAG	2 cut(s)	191, 1707
BseNI	ACTGG	6 cut(s)	221, 697, 1368, 1475, 1935, 2026
BseRI	GAGGAG	1 cut(s)	720
BseXI	GCAGC	5 cut(s)	433, 436, 1265, 1616, 1619
BshFI	GGCC	6 cut(s)	696, 924, 1087, 1118, 1441, 2025
BshNI	GGYRCC	2 cut(s)	766, 1755
BsiHKAI	GWGCWC	2 cut(s)	1071, 2285
BsiHKCI	CYCGRG	2 cut(s)	411, 1444
BsiSI	CCGG	3 cut(s)	106, 770, 1329
BslFI	GGGAC	2 cut(s)	452, 562
BslI	CCNNNNNNNGG	7 cut(s)	306, 776, 985, 986, 1535, 1743, 2206
BsmAI	GTCTC	2 cut(s)	8, 2075
BsmFI	GGGAC	2 cut(s)	452, 562
BsnI	GGCC	6 cut(s)	696, 924, 1087, 1118, 1441, 2025
Bso31I	GGTCTC	1 cut(s)	8
BsoBI	CYCGRG	2 cut(s)	411, 1444
Bsp1286I	GDGCHC	2 cut(s)	1071, 2285
Bsp1407I	TGTACA	1 cut(s)	2076
Bsp1720I	GCTNAGC	1 cut(s)	1703
Bsp19I	CCATGG	2 cut(s)	462, 1770
BspACI	CCGC	4 cut(s)	5, 697, 758, 2307
BspANI	GGCC	6 cut(s)	696, 924, 1087, 1118, 1441, 2025
BspCNI	CTCAG	2 cut(s)	190, 1708
BspHI	TCATGA	1 cut(s)	792
BspMI	ACCTGC	1 cut(s)	411
BspPI	GGATC	9 cut(s)	97, 146, 291, 304, 1013, 1177, 1546, 2239, 2252
BspQI	GCTCTTC	2 cut(s)	116, 2297
BspT107I	GGYRCC	2 cut(s)	766, 1755
BspTI	CTTAAG	1 cut(s)	2014
BspTNI	GGTCTC	1 cut(s)	8
BsrBI	CCGCTC	1 cut(s)	2307
BsrDI	GCAATG	1 cut(s)	717
BsrFI	RCCGGY	1 cut(s)	769
BsrGI	TGTACA	1 cut(s)	2076
BsrI	ACTGG	6 cut(s)	221, 697, 1368, 1475, 1935, 2026
BssAI	RCCGGY	1 cut(s)	769
BssECI	CCNNGG	7 cut(s)	274, 339, 462, 1770, 1830, 2248, 2324
BssNI	GRCGYC	1 cut(s)	87
BssSI	CACGAG	1 cut(s)	143
BssT1I	CCWWGG	3 cut(s)	462, 1770, 2324
Bst2BI	CACGAG	1 cut(s)	143
Bst2UI	CCWGG	2 cut(s)	235, 276
Bst4CI	ACNGT	5 cut(s)	268, 1384, 1786, 1831, 2114
Bst6I	CTCTTC	5 cut(s)	116, 587, 709, 2175, 2297
BstACI	GRCGYC	1 cut(s)	87
BstAFI	CTTAAG	1 cut(s)	2014
BstAUI	TGTACA	1 cut(s)	2076
BstC8I	GCNNGC	6 cut(s)	42, 926, 1033, 1147, 1171, 1258
BstDEI	CTNAG	4 cut(s)	177, 1007, 1703, 1716
BstDSI	CCRYGG	4 cut(s)	339, 462, 1770, 1830
BstF5I	GGATG	8 cut(s)	69, 75, 995, 1729, 1894, 1968, 2026, 2211
BstMAI	GTCTC	2 cut(s)	8, 2075
BstMWI	GCNNNNNNNGC	9 cut(s)	458, 527, 947, 968, 1234, 1253, 1613, 1622, 2031
BstNI	CCWGG	2 cut(s)	235, 276
BstNSI	RCATGY	3 cut(s)	674, 842, 1149
BstSCI	CCNGG	3 cut(s)	233, 274, 1327
BstSFI	CTRYAG	1 cut(s)	516
BstV1I	GCAGC	5 cut(s)	433, 436, 1265, 1616, 1619
BstV2I	GAAGAC	3 cut(s)	1325, 1655, 1839
BstX2I	RGATCY	3 cut(s)	296, 1538, 2244
BstYI	RGATCY	3 cut(s)	296, 1538, 2244
BsuI	GTATCC	3 cut(s)	249, 925, 1396
BsuRI	GGCC	6 cut(s)	696, 924, 1087, 1118, 1441, 2025
BtgI	CCRYGG	4 cut(s)	339, 462, 1770, 1830
BtsCI	GGATG	8 cut(s)	69, 75, 995, 1729, 1894, 1968, 2026, 2211
BtsIMutI	CAGTG	5 cut(s)	495, 1380, 1713, 1782, 1942
BveI	ACCTGC	1 cut(s)	411
Cac8I	GCNNGC	6 cut(s)	42, 926, 1033, 1147, 1171, 1258
CaiI	CAGNNNCTG	3 cut(s)	184, 449, 494
CciI	TCATGA	1 cut(s)	792
Cfr10I	RCCGGY	1 cut(s)	769
Cfr13I	GGNCC	5 cut(s)	466, 576, 1086, 1440, 1966
CseI	GACGC	1 cut(s)	76
CsiI	ACCWGGT	1 cut(s)	233
DdeI	CTNAG	4 cut(s)	177, 1007, 1703, 1716
DraIII	CACNNNGTG	1 cut(s)	390
DrdI	GACNNNNNNGTC	1 cut(s)	2263
DseDI	GACNNNNNNGTC	1 cut(s)	2263
EaeI	YGGCCR	2 cut(s)	694, 2023
Eam1104I	CTCTTC	5 cut(s)	116, 587, 709, 2175, 2297
EarI	CTCTTC	5 cut(s)	116, 587, 709, 2175, 2297
Ecl136II	GAGCTC	1 cut(s)	2283
Eco130I	CCWWGG	3 cut(s)	462, 1770, 2324
Eco24I	GRGCYC	1 cut(s)	2285
Eco31I	GGTCTC	1 cut(s)	8
Eco47I	GGWCC	3 cut(s)	466, 576, 1966
Eco53kI	GAGCTC	1 cut(s)	2283
Eco57I	CTGAAG	1 cut(s)	1305
Eco88I	CYCGRG	2 cut(s)	411, 1444
EcoICRI	GAGCTC	1 cut(s)	2283
EcoO109I	RGGNCCY	2 cut(s)	576, 1440
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	2 cut(s)	233, 274
EcoT14I	CCWWGG	3 cut(s)	462, 1770, 2324
EcoT22I	ATGCAT	1 cut(s)	672
EcoT38I	GRGCYC	1 cut(s)	2285
ErhI	CCWWGG	3 cut(s)	462, 1770, 2324
FalI	AAGNNNNNCTT	1 cut(s)	2308
FaqI	GGGAC	2 cut(s)	452, 562
FauNDI	CATATG	1 cut(s)	724
FblI	GTMKAC	1 cut(s)	1012
Fnu4HI	GCNGC	8 cut(s)	6, 447, 450, 697, 759, 1254, 1605, 1608
FokI	GGATG	8 cut(s)	56, 62, 1002, 1736, 1901, 1975, 2033, 2218
FriOI	GRGCYC	1 cut(s)	2285
Fsp4HI	GCNGC	8 cut(s)	6, 447, 450, 697, 759, 1254, 1605, 1608
FspBI	CTAG	4 cut(s)	929, 1056, 1238, 1337
GluI	GCNGC	8 cut(s)	6, 447, 450, 697, 759, 1254, 1605, 1608
GsuI	CTGGAG	3 cut(s)	1233, 1236, 1836
HaeIII	GGCC	6 cut(s)	696, 924, 1087, 1118, 1441, 2025
HapII	CCGG	3 cut(s)	106, 770, 1329
HgaI	GACGC	1 cut(s)	76
Hin1I	GRCGYC	1 cut(s)	87
HincII	GTYRAC	1 cut(s)	1795
HindII	GTYRAC	1 cut(s)	1795
HindIII	AAGCTT	3 cut(s)	1287, 1614, 1863
HinfI	GANTC	7 cut(s)	171, 409, 562, 780, 871, 1218, 2320
HpaII	CCGG	3 cut(s)	106, 770, 1329
HphI	GGTGA	3 cut(s)	1146, 2143, 2189
Hpy166II	GTNNAC	6 cut(s)	271, 856, 1013, 1795, 2104, 2190
Hpy188III	TCNNGA	7 cut(s)	79, 143, 413, 793, 1215, 1446, 1853
Hpy8I	GTNNAC	6 cut(s)	271, 856, 1013, 1795, 2104, 2190
HpyAV	CCTTC	7 cut(s)	73, 826, 1129, 1353, 1456, 1497, 1588
HpyCH4III	ACNGT	5 cut(s)	268, 1384, 1786, 1831, 2114
HpyCH4IV	ACGT	2 cut(s)	749, 1296
HpyF10VI	GCNNNNNNNGC	9 cut(s)	458, 527, 947, 968, 1234, 1253, 1613, 1622, 2031
HpyF3I	CTNAG	4 cut(s)	177, 1007, 1703, 1716
HpySE526I	ACGT	2 cut(s)	749, 1296
Hsp92I	GRCGYC	1 cut(s)	87
KflI	GGGWCCC	1 cut(s)	576
KpnI	GGTACC	1 cut(s)	1759
LguI	GCTCTTC	2 cut(s)	116, 2297
LmnI	GCTCC	3 cut(s)	733, 1252, 1574
Lsp1109I	GCAGC	5 cut(s)	433, 436, 1265, 1616, 1619
LweI	GCATC	4 cut(s)	17, 1156, 1269, 1345
MabI	ACCWGGT	1 cut(s)	233
MaeI	CTAG	4 cut(s)	929, 1056, 1238, 1337
MaeII	ACGT	2 cut(s)	749, 1296
MaeIII	GTNAC	5 cut(s)	172, 688, 901, 1195, 1323
MbiI	CCGCTC	1 cut(s)	2307
MflI	RGATCY	3 cut(s)	296, 1538, 2244
MhlI	GDGCHC	2 cut(s)	1071, 2285
MlsI	TGGCCA	1 cut(s)	2025
MluCI	AATT	8 cut(s)	163, 316, 1047, 1137, 1503, 1872, 1971, 2065
MluNI	TGGCCA	1 cut(s)	2025
MlyI	GAGTC	4 cut(s)	180, 403, 556, 1227
MmeI	TCCRAC	4 cut(s)	45, 757, 1046, 1866
Mox20I	TGGCCA	1 cut(s)	2025
Mph1103I	ATGCAT	1 cut(s)	672
MroXI	GAANNNNTTC	2 cut(s)	887, 2159
MscI	TGGCCA	1 cut(s)	2025
MseI	TTAA	9 cut(s)	420, 597, 654, 1107, 1430, 1494, 1947, 2015, 2332
MslI	CAYNNNNRTG	2 cut(s)	1653, 1718
Msp20I	TGGCCA	1 cut(s)	2025
MspA1I	CMGCKG	2 cut(s)	446, 1133
MspCI	CTTAAG	1 cut(s)	2014
MspI	CCGG	3 cut(s)	106, 770, 1329
MspR9I	CCNGG	3 cut(s)	235, 276, 1329
MvaI	CCWGG	2 cut(s)	235, 276
MwoI	GCNNNNNNNGC	9 cut(s)	458, 527, 947, 968, 1234, 1253, 1613, 1622, 2031
NciI	CCSGG	1 cut(s)	1329
NcoI	CCATGG	2 cut(s)	462, 1770
NdeI	CATATG	1 cut(s)	724
NmuCI	GTSAC	2 cut(s)	172, 901
NsiI	ATGCAT	1 cut(s)	672
NspI	RCATGY	3 cut(s)	674, 842, 1149
OliI	CACNNNNGTG	1 cut(s)	1718
PaeI	GCATGC	1 cut(s)	1149
PaeR7I	CTCGAG	1 cut(s)	1444
PagI	TCATGA	1 cut(s)	792
PciI	ACATGT	1 cut(s)	838
PciSI	GCTCTTC	2 cut(s)	116, 2297
PcsI	WCGNNNNNNNCGW	1 cut(s)	2285
PdmI	GAANNNNTTC	2 cut(s)	887, 2159
PfeI	GAWTC	3 cut(s)	780, 871, 2320
PflMI	CCANNNNNTGG	1 cut(s)	2206
PkrI	GCNGC	8 cut(s)	7, 448, 451, 698, 760, 1255, 1606, 1609
PleI	GAGTC	4 cut(s)	179, 403, 556, 1226
PpsI	GAGTC	4 cut(s)	179, 403, 556, 1226
PpuMI	RGGWCCY	1 cut(s)	576
PscI	ACATGT	1 cut(s)	838
PshBI	ATTAAT	1 cut(s)	1107
PsiI	TTATAA	1 cut(s)	122
Psp124BI	GAGCTC	1 cut(s)	2285
Psp1406I	AACGTT	1 cut(s)	749
Psp5II	RGGWCCY	1 cut(s)	576
Psp6I	CCWGG	2 cut(s)	233, 274
PspGI	CCWGG	2 cut(s)	233, 274
PspPI	GGNCC	5 cut(s)	466, 576, 1086, 1440, 1966
PspPPI	RGGWCCY	1 cut(s)	576
PstNI	CAGNNNCTG	3 cut(s)	184, 449, 494
PsuI	RGATCY	3 cut(s)	296, 1538, 2244
PvuII	CAGCTG	2 cut(s)	446, 1133
RseI	CAYNNNNRTG	2 cut(s)	1653, 1718
SacI	GAGCTC	1 cut(s)	2285
SapI	GCTCTTC	2 cut(s)	116, 2297
SaqAI	TTAA	9 cut(s)	420, 597, 654, 1107, 1430, 1494, 1947, 2015, 2332
SatI	GCNGC	8 cut(s)	6, 447, 450, 697, 759, 1254, 1605, 1608
Sau96I	GGNCC	5 cut(s)	466, 576, 1086, 1440, 1966
ScaI	AGTACT	2 cut(s)	954, 1242
SchI	GAGTC	4 cut(s)	180, 403, 556, 1227
ScrFI	CCNGG	3 cut(s)	235, 276, 1329
SduI	GDGCHC	2 cut(s)	1071, 2285
SexAI	ACCWGGT	1 cut(s)	233
SfaNI	GCATC	4 cut(s)	17, 1156, 1269, 1345
SfcI	CTRYAG	1 cut(s)	516
Sfr274I	CTCGAG	1 cut(s)	1444
SinI	GGWCC	3 cut(s)	466, 576, 1966
SlaI	CTCGAG	1 cut(s)	1444
SmiMI	CAYNNNNRTG	2 cut(s)	1653, 1718
SmlI	CTYRAG	3 cut(s)	34, 1444, 2014
SmoI	CTYRAG	3 cut(s)	34, 1444, 2014
SphI	GCATGC	1 cut(s)	1149
Sse9I	AATT	8 cut(s)	163, 316, 1047, 1137, 1503, 1872, 1971, 2065
SsiI	CCGC	4 cut(s)	5, 697, 758, 2307
SspMI	CTAG	4 cut(s)	929, 1056, 1238, 1337
SstI	GAGCTC	1 cut(s)	2285
StyD4I	CCNGG	3 cut(s)	233, 274, 1327
StyI	CCWWGG	3 cut(s)	462, 1770, 2324
TaaI	ACNGT	5 cut(s)	268, 1384, 1786, 1831, 2114
TaiI	ACGT	2 cut(s)	752, 1299
TaqI	TCGA	4 cut(s)	101, 1445, 2273, 2279
TaqII	GACCGA	2 cut(s)	243, 474
TasI	AATT	8 cut(s)	163, 316, 1047, 1137, 1503, 1872, 1971, 2065
TatI	WGTACW	5 cut(s)	855, 952, 1038, 1240, 2076
TauI	GCSGC	3 cut(s)	8, 699, 761
TfiI	GAWTC	3 cut(s)	780, 871, 2320
Tru1I	TTAA	9 cut(s)	420, 597, 654, 1107, 1430, 1494, 1947, 2015, 2332
Tru9I	TTAA	9 cut(s)	420, 597, 654, 1107, 1430, 1494, 1947, 2015, 2332
TscAI	CASTG	5 cut(s)	495, 1387, 1720, 1789, 1942
TseFI	GTSAC	2 cut(s)	172, 901
TseI	GCWGC	5 cut(s)	446, 449, 1253, 1604, 1607
Tsp45I	GTSAC	2 cut(s)	172, 901
TspDTI	ATGAA	6 cut(s)	58, 309, 525, 1410, 1580, 1655
TspGWI	ACGGA	3 cut(s)	39, 144, 328
TspRI	CASTG	5 cut(s)	495, 1387, 1720, 1789, 1942
Van91I	CCANNNNNTGG	1 cut(s)	2206
Vha464I	CTTAAG	1 cut(s)	2014
VpaK11BI	GGWCC	3 cut(s)	466, 576, 1966
VspI	ATTAAT	1 cut(s)	1107
XapI	RAATTY	2 cut(s)	316, 1872
XceI	RCATGY	3 cut(s)	674, 842, 1149
XcmI	CCANNNNNNNNNTGG	1 cut(s)	987
XhoI	CTCGAG	1 cut(s)	1444
XmiI	GTMKAC	1 cut(s)	1012
XmnI	GAANNNNTTC	2 cut(s)	887, 2159
XspI	CTAG	4 cut(s)	929, 1056, 1238, 1337
ZrmI	AGTACT	2 cut(s)	954, 1242
Zsp2I	ATGCAT	1 cut(s)	672

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.