MD02G1124300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017217
Physical Location & Seq
Forward (+)
10125856 .. 10127200
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1124300.v1.1.491

Sequence Viewer

Length: 414 bp
ATGCACCAATTAGGCGTGAAGAAAAGCAGCAATCTTCTTCTGGGTCCTGGTTCTGCTGCCGTGGCAAACTCTGCAGGCAATGCCCAGCCTCTTTGCCCTAAGCCTCGAAGAGCTGGCCTTGCTATGCCTGAGTTCCTCAACCCTATAAGATGTAACAAGCACAGCCAAAAAAATTCCGATGAGAGAAGTGGGGTTCTGAATATGATCAGTGAAAAGGAAATATATGGTGGAAGGGAGTATGTGTGCACGAAATGCTCACCGGCATGCTACTCAGGCTCTCCACCAGGAAGAACACACAACCCTTTGGTTCATGATGTGCAGTTCCTTCATCAGATGGAGCTTCCTTCTCCACTTACAAGAACAAAGCTCTCAGAAAAATTTGGTTTTAGTACAACCTCTGCCTCTCCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

14.79

Weight (kDa)

9.4

Isoelectric Point (pI)

61.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11180
malus_domestica MD02G1124300.v1.1 MD15G1238300.v1.1
prunus_persica Prupe.7G174100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0098471
rosa_laevigata RLG00000016802
rosa_roxburghii Rroxscaffold_2G00143930
rosa_rugosa Rorug02G0073500
rosa_samantha Rh2AG120800 Rh2BG123600 Rh2CG125200 Rh2DG125800
rosa_wichuraiana Rw2G009470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 172, 377
AfaI GTAC 1 cut(s) 391
AjnI CCWGG 2 cut(s) 46, 283
AluBI AGCT 3 cut(s) 113, 340, 367
AluI AGCT 3 cut(s) 113, 340, 367
Alw21I GWGCWC 1 cut(s) 248
Alw44I GTGCAC 1 cut(s) 244
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 27, 56
ApoI RAATTY 2 cut(s) 172, 377
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 1 cut(s) 249
AvaII GGWCC 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 2 cut(s) 39, 43
BccI CCATC 1 cut(s) 328
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 2 cut(s) 48, 285
BclI TGATCA 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 72
BisI GCNGC 2 cut(s) 28, 57
BlsI GCNGC 2 cut(s) 29, 58
Bme1390I CCNGG 2 cut(s) 48, 285
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 2 cut(s) 48, 285
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bse118I RCCGGY 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 85
BseBI CCWGG 2 cut(s) 48, 285
BseDI CCNNGG 1 cut(s) 60
BseMI GCAATG 1 cut(s) 85
BseMII CTCAG 3 cut(s) 120, 285, 384
BseNI ACTGG 1 cut(s) 407
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 2 cut(s) 39, 43
BseYI CCCAGC 1 cut(s) 84
BsgI GTGCAG 1 cut(s) 338
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 248
BsiSI CCGG 1 cut(s) 260
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 1 cut(s) 204
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 3 cut(s) 121, 284, 383
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 45
BspMAI CTGCAG 1 cut(s) 76
BspQI GCTCTTC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 259
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 1 cut(s) 204
Bst2UI CCWGG 2 cut(s) 48, 285
Bst6I CTCTTC 1 cut(s) 103
BstAPI GCANNNNNTGC 3 cut(s) 71, 80, 252
BstC8I GCNNGC 3 cut(s) 76, 115, 265
BstDEI CTNAG 4 cut(s) 99, 129, 271, 370
BstDSI CCRYGG 1 cut(s) 60
BstKTI GATC 1 cut(s) 207
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 6 cut(s) 62, 71, 80, 119, 252, 273
BstNI CCWGG 2 cut(s) 48, 285
BstNSI RCATGY 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 46, 283
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 39, 43
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 214, 414
Cac8I GCNNGC 3 cut(s) 76, 115, 265
CciI TCATGA 1 cut(s) 310
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 390
CviAII CATG 2 cut(s) 264, 311
CviJI RGCY 8 cut(s) 88, 103, 113, 117, 165, 276, 340, 367
CviKI_1 RGCY 8 cut(s) 88, 103, 113, 117, 165, 276, 340, 367
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 4 cut(s) 99, 129, 271, 370
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 46, 283
FaeI CATG 2 cut(s) 267, 314
FaiI YATR 8 cut(s) 125, 146, 203, 223, 225, 240, 265, 312
FatI CATG 2 cut(s) 263, 310
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 28, 57
Fsp4HI GCNGC 2 cut(s) 28, 57
GluI GCNGC 2 cut(s) 28, 57
GsaI CCCAGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 260
Hin1II CATG 2 cut(s) 267, 314
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 249
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 4 cut(s) 178, 198, 333, 373
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 3 cut(s) 225, 335, 354
HpyCH4V TGCA 4 cut(s) 4, 74, 246, 319
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 71, 80, 119, 252, 273
HpyF3I CTNAG 4 cut(s) 99, 129, 271, 370
Hsp92II CATG 2 cut(s) 267, 314
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 1 cut(s) 204
LguI GCTCTTC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 2 cut(s) 39, 43
MaeIII GTNAC 1 cut(s) 152
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 5 cut(s) 26, 29, 31, 120, 300
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 3 cut(s) 8, 172, 377
MnlI CCTC 5 cut(s) 99, 114, 146, 406, 412
MslI CAYNNNNRTG 1 cut(s) 262
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 2 cut(s) 48, 285
MvaI CCWGG 2 cut(s) 48, 285
MwoI GCNNNNNNNGC 6 cut(s) 62, 71, 80, 119, 252, 273
NdeII GATC 1 cut(s) 204
NlaIII CATG 2 cut(s) 267, 314
NlaIV GGNNCC 1 cut(s) 45
NspI RCATGY 1 cut(s) 267
PaeI GCATGC 1 cut(s) 267
PagI TCATGA 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 29, 58
PpuMI RGGWCCY 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 2 cut(s) 46, 283
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 2 cut(s) 46, 283
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 1 cut(s) 44
PspPPI RGGWCCY 1 cut(s) 44
PstI CTGCAG 1 cut(s) 76
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 262
SapI GCTCTTC 1 cut(s) 103
SatI GCNGC 2 cut(s) 28, 57
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 48, 285
SduI GDGCHC 1 cut(s) 248
SetI ASST 4 cut(s) 115, 342, 369, 398
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 262
SphI GCATGC 1 cut(s) 267
Sse9I AATT 3 cut(s) 8, 172, 377
StyD4I CCNGG 2 cut(s) 46, 283
TaqI TCGA 1 cut(s) 106
TasI AATT 3 cut(s) 8, 172, 377
TatI WGTACW 1 cut(s) 389
TscAI CASTG 2 cut(s) 214, 414
TseI GCWGC 2 cut(s) 27, 56
TspDTI ATGAA 2 cut(s) 299, 317
TspRI CASTG 2 cut(s) 214, 414
VneI GTGCAC 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 44
XapI RAATTY 2 cut(s) 172, 377
XceI RCATGY 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.