Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014399

Physical Location & Seq

Genomic Coordinates Reverse (-)

12447969 .. 12450287

Gene Structure 2319 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1151000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGCCAGAGAATATTACTACATCCCCGATGGAGGTGGAGGGCAGTGGCGAAAGCCATGAAGCCCCGGAAAGGATCAACAAGATTTCTGTTGCTTGTGCAGTGGTTGCCTCCATTATATCTATTATATTTGGCTATGATACTGGTGTGATGAGTGGAGCCATGATCTTCATCAAGGAAGACCTCAAAATCGACGATGTACAAGTCGAAGTACTTGCAGGAATACTAAACATTTGTGCTCTAGTGGGATCCTTAGCAGCTGGAAGAACATCTGATTACATCGGTCGGCGTTATACTATTGTTCTAGCCTCCACTATTTTCTTAATAGGTGGAGTGCTAATGGGGTATGGTCCAAACTATGCAGTCCTAATGACTGGGAGGTGCACTGCTGGCATTGGTGTAGGGTTTGCACTAATGATTGCTCCAGTTTACTCTGCAGAAATTTCATCTGCCAAATCAAGAGGCCTTCTAACCTCGCTCCCGGGGTTTTGCATAAGTCTTGGAATCTTAATAGGGTACGTGTCAAATTATTTGTTCGGAAAACTGACTTTGAAACTCGGATGGAGAATTATGCTAGGAGTTTCTGGAATTCCTTCAATTGCCTTAGCTTTTGGGATTCTCAAAATGCCCGAGTCCCCAAGGTGGTTGGTGATGCAAGGTAGGCTAGCAGAAGCTAAGAAAACATTGTTACTAGTTTGTAATTCCAAAGAAGAAGCCGAGCTTCGTTTTCGCGATATCTTGGTTGCTGCTGGAATCGACGAAAATTGCAACGAAGATGTGGTGAAGGTCAGTAATACTACTAGGGGTGAAGGGGCTTGGAAAGAACTATTATTGAGGCCGAAACCCGCTGTCCGCCGGATTTTCATTGCAGCGGTTGGAATCCACTTTTTTCAGCATGCGACAGGGATAGAAGCTGTTGTGTTATATGGTCCAAGAATTTTCAAAAAAGCTGGGGTCGTTGGCAAAGACAAGTTATTGCTTGCCACAGTTGGAGTTGGGGTCACAAAACTTTTGTTTATAGGTGTAGCCACAACGTTCCTGGACAAAGCTGGGAGGAGGCCCCTACTGTTGATCAGCACTGGTGGCATGATTATAGCCCTAGCAAGTTTAGGGTTTGGTTTGACCATGGCCGAGCACTCCAAAGAGAAACATACTTGGCCTCTAAGCTTCAGTATCGTGGCAACTTATGTGTTTGTGTCATTCTTCTCAATTGGCCTGGGGCCCATAACATGGGTTTACAGCACTGAGATTTTTCCTTTGAATCTTAGGGCACAAGGTGCTAGTATTGGGGTGGCTGTGAATAGGCTCTTGAATGCTACAGTTGCTATGAGCTTCATTTCAATTTACAAGGCAATTACAATTGGAGGGGCCTTCTTTATGTTTGCAGGCATAGCTGTTATAGCTTGGTTATTTTTCTTTTTCTTCTTACCAGAGACCAAGGGAAGAACCTTGGAAGAGATGGAAATGGTATTCAGCAAAAATAGTGATGCTAGTAGGTCTAAAAATGGTACCGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0022804 GO:0022857 GO:0022890 GO:0034219 GO:0034220 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098655 GO:0098660 GO:0098662 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

505

Amino Acids

54.17

Weight (kDa)

9.22

Isoelectric Point (pI)

29.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	32 - 490	1.8e-104	Sugar (and other) transporter
MFS_1	PF07690	37 - 435	2.2e-28	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014399)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18480 AT4G36670
fragaria_vesca	FvH4_1g13630
malus_domestica	MD02G1151000.v1.1 MD15G1265500.v1.1
prunus_persica	Prupe.7G152100_v2.0.a1
pyrus_communis	pycom15g23120
rosa_chinensis	RchiOBHm_Chr2g0101981
rosa_laevigata	RLG00000017122
rosa_multiflora	Rmu_sc0020823.1_g000008
rosa_roxburghii	Rroxscaffold_2G00140870
rosa_rugosa	Rorug02G0103700
rosa_samantha	Rh2AG151700 Rh2BG157100 Rh2CG157100 Rh2DG157200
rosa_wichuraiana	Rw2G011840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1505
AccB1I	GGYRCC	1 cut(s)	1505
AccB7I	CCANNNNNTGG	1 cut(s)	1227
AccII	CGCG	1 cut(s)	729
AciI	CCGC	3 cut(s)	843, 850, 869
AclI	AACGTT	1 cut(s)	1031
AclWI	GGATC	3 cut(s)	80, 240, 253
AcoI	YGGCCR	1 cut(s)	1125
AcsI	RAATTY	3 cut(s)	438, 585, 933
AcuI	CTGAAG	1 cut(s)	1150
AdeI	CACNNNGTG	1 cut(s)	1274
AfaI	GTAC	4 cut(s)	198, 210, 515, 1507
AfiI	CCNNNNNNNGG	4 cut(s)	31, 69, 639, 1227
AflIII	ACRYGT	1 cut(s)	516
AgsI	TTSAA	6 cut(s)	550, 594, 940, 1258, 1309, 1338
AhlI	ACTAGT	1 cut(s)	688
AjnI	CCWGG	2 cut(s)	1035, 1212
Alw21I	GWGCWC	3 cut(s)	238, 383, 1134
Alw26I	GTCTC	1 cut(s)	1424
Alw44I	GTGCAC	1 cut(s)	379
AlwI	GGATC	3 cut(s)	80, 240, 253
Ama87I	CYCGRG	2 cut(s)	478, 626
AoxI	GGCC	8 cut(s)	460, 833, 1055, 1125, 1154, 1210, 1217, 1365
ApaI	GGGCCC	1 cut(s)	1221
ApaLI	GTGCAC	1 cut(s)	379
ApeKI	GCWGC	3 cut(s)	254, 743, 866
ApoI	RAATTY	3 cut(s)	438, 585, 933
Asp700I	GAANNNNTTC	1 cut(s)	589
Asp718I	GGTACC	1 cut(s)	1505
AspS9I	GGNCC	6 cut(s)	347, 926, 1056, 1217, 1218, 1365
AsuC2I	CCSGG	3 cut(s)	65, 479, 480
AsuHPI	GGTGA	3 cut(s)	658, 790, 815
AsuNHI	GCTAGC	1 cut(s)	661
AvaI	CYCGRG	2 cut(s)	478, 626
AvaII	GGWCC	2 cut(s)	347, 926
BaeGI	GKGCMC	3 cut(s)	383, 1221, 1270
BaeI	ACNNNNGTAYC	2 cut(s)	129, 162
BamHI	GGATCC	1 cut(s)	245
BanI	GGYRCC	1 cut(s)	1505
BanII	GRGCYC	1 cut(s)	1221
BbsI	GAAGAC	1 cut(s)	183
Bbv12I	GWGCWC	3 cut(s)	238, 383, 1134
BbvI	GCAGC	3 cut(s)	266, 730, 878
BccI	CCATC	3 cut(s)	22, 552, 1450
BcgI	CGANNNNNNTGC	2 cut(s)	194, 228
BciT130I	CCWGG	2 cut(s)	1037, 1214
BclI	TGATCA	1 cut(s)	1068
BcnI	CCSGG	3 cut(s)	65, 479, 480
BcoDI	GTCTC	1 cut(s)	1424
BcuI	ACTAGT	1 cut(s)	688
BfaI	CTAG	9 cut(s)	239, 302, 572, 662, 689, 798, 1097, 1278, 1488
BfmI	CTRYAG	2 cut(s)	432, 1314
BisI	GCNGC	3 cut(s)	255, 744, 867
BlsI	GCNGC	3 cut(s)	256, 745, 868
BmcAI	AGTACT	1 cut(s)	210
Bme1390I	CCNGG	5 cut(s)	65, 479, 480, 1037, 1214
Bme18I	GGWCC	2 cut(s)	347, 926
BmeT110I	CYCGRG	2 cut(s)	478, 626
BmgT120I	GGNCC	6 cut(s)	347, 926, 1056, 1217, 1218, 1365
BmiI	GGNNCC	7 cut(s)	157, 247, 1058, 1218, 1219, 1366, 1507
BmrFI	CCNGG	5 cut(s)	65, 479, 480, 1037, 1214
BmrI	ACTGGG	1 cut(s)	381
BmsI	GCATC	2 cut(s)	639, 1474
BmtI	GCTAGC	1 cut(s)	665
BmuI	ACTGGG	1 cut(s)	381
BpiI	GAAGAC	1 cut(s)	183
BpmI	CTGGAG	1 cut(s)	405
Bpu10I	CCTNAGC	2 cut(s)	250, 601
BpuMI	CCSGG	3 cut(s)	65, 479, 480
BsaAI	YACGTR	1 cut(s)	517
BsaBI	GATNNNNATC	1 cut(s)	167
BsaI	GGTCTC	1 cut(s)	1424
BsaJI	CCNNGG	8 cut(s)	63, 478, 479, 635, 1122, 1213, 1434, 1446
BsaXI	ACNNNNNCTCC	2 cut(s)	981, 1011
Bsc4I	CCNNNNNNNGG	4 cut(s)	31, 69, 639, 1227
Bse1I	ACTGG	4 cut(s)	145, 376, 422, 1081
Bse3DI	GCAATG	1 cut(s)	861
Bse8I	GATNNNNATC	1 cut(s)	167
BseBI	CCWGG	2 cut(s)	1037, 1214
BseDI	CCNNGG	8 cut(s)	63, 478, 479, 635, 1122, 1213, 1434, 1446
BseGI	GGATG	2 cut(s)	20, 563
BseJI	GATNNNNATC	1 cut(s)	167
BseLI	CCNNNNNNNGG	4 cut(s)	31, 69, 639, 1227
BseMI	GCAATG	1 cut(s)	861
BseMII	CTCAG	1 cut(s)	1233
BseNI	ACTGG	4 cut(s)	145, 376, 422, 1081
BseRI	GAGGAG	1 cut(s)	1066
BseSI	GKGCMC	3 cut(s)	383, 1221, 1270
BseXI	GCAGC	3 cut(s)	266, 730, 878
BseYI	CCCAGC	2 cut(s)	947, 1046
BsgI	GTGCAG	1 cut(s)	117
Bsh1236I	CGCG	1 cut(s)	729
Bsh1285I	CGRYCG	1 cut(s)	283
BshFI	GGCC	8 cut(s)	462, 835, 1057, 1127, 1156, 1212, 1219, 1367
BshNI	GGYRCC	1 cut(s)	1505
BsiEI	CGRYCG	1 cut(s)	283
BsiHKAI	GWGCWC	3 cut(s)	238, 383, 1134
BsiHKCI	CYCGRG	2 cut(s)	478, 626
BsiSI	CCGG	3 cut(s)	65, 479, 853
BslFI	GGGAC	1 cut(s)	616
BslI	CCNNNNNNNGG	4 cut(s)	31, 69, 639, 1227
BsmAI	GTCTC	1 cut(s)	1424
BsmFI	GGGAC	1 cut(s)	616
BsmI	GAATGC	1 cut(s)	1315
BsnI	GGCC	8 cut(s)	462, 835, 1057, 1127, 1156, 1212, 1219, 1367
Bso31I	GGTCTC	1 cut(s)	1424
BsoBI	CYCGRG	2 cut(s)	478, 626
Bsp120I	GGGCCC	1 cut(s)	1217
Bsp1286I	GDGCHC	5 cut(s)	238, 383, 1134, 1221, 1270
Bsp1407I	TGTACA	1 cut(s)	196
Bsp143I	GATC	4 cut(s)	72, 162, 245, 1068
Bsp19I	CCATGG	1 cut(s)	1122
Bsp68I	TCGCGA	1 cut(s)	729
BspACI	CCGC	3 cut(s)	843, 850, 869
BspANI	GGCC	8 cut(s)	462, 835, 1057, 1127, 1156, 1212, 1219, 1367
BspCNI	CTCAG	1 cut(s)	1234
BspFNI	CGCG	1 cut(s)	729
BspLI	GGNNCC	7 cut(s)	157, 247, 1058, 1218, 1219, 1366, 1507
BspMAI	CTGCAG	1 cut(s)	436
BspOI	GCTAGC	1 cut(s)	665
BspPI	GGATC	3 cut(s)	80, 240, 253
BspT107I	GGYRCC	1 cut(s)	1505
BspTNI	GGTCTC	1 cut(s)	1424
BsrDI	GCAATG	1 cut(s)	861
BsrGI	TGTACA	1 cut(s)	196
BsrI	ACTGG	4 cut(s)	145, 376, 422, 1081
BssECI	CCNNGG	8 cut(s)	63, 478, 479, 635, 1122, 1213, 1434, 1446
BssMI	GATC	4 cut(s)	72, 162, 245, 1068
BssT1I	CCWWGG	4 cut(s)	635, 1122, 1434, 1446
Bst2UI	CCWGG	2 cut(s)	1037, 1214
Bst4CI	ACNGT	4 cut(s)	985, 1065, 1318, 1510
Bst6I	CTCTTC	1 cut(s)	1446
BstAPI	GCANNNNNTGC	2 cut(s)	104, 1274
BstAUI	TGTACA	1 cut(s)	196
BstBAI	YACGTR	1 cut(s)	517
BstC8I	GCNNGC	5 cut(s)	388, 663, 894, 978, 1384
BstDEI	CTNAG	6 cut(s)	250, 601, 672, 1160, 1242, 1262
BstDSI	CCRYGG	1 cut(s)	1122
BstF5I	GGATG	2 cut(s)	20, 563
BstFNI	CGCG	1 cut(s)	729
BstKTI	GATC	4 cut(s)	75, 165, 248, 1071
BstMAI	GTCTC	1 cut(s)	1424
BstMBI	GATC	4 cut(s)	72, 162, 245, 1068
BstMCI	CGRYCG	1 cut(s)	283
BstMWI	GCNNNNNNNGC	8 cut(s)	104, 387, 658, 1080, 1274, 1319, 1388, 1397
BstNI	CCWGG	2 cut(s)	1037, 1214
BstNSI	RCATGY	1 cut(s)	896
BstSCI	CCNGG	5 cut(s)	63, 477, 478, 1035, 1212
BstSFI	CTRYAG	2 cut(s)	432, 1314
BstSLI	GKGCMC	3 cut(s)	383, 1221, 1270
BstUI	CGCG	1 cut(s)	729
BstV1I	GCAGC	3 cut(s)	266, 730, 878
BstV2I	GAAGAC	1 cut(s)	183
BstX2I	RGATCY	1 cut(s)	245
BstYI	RGATCY	1 cut(s)	245
BsuRI	GGCC	8 cut(s)	462, 835, 1057, 1127, 1156, 1212, 1219, 1367
BtgI	CCRYGG	1 cut(s)	1122
BtsCI	GGATG	2 cut(s)	20, 563
BtsI	GCAGTG	3 cut(s)	49, 105, 381
BtsIMutI	CAGTG	5 cut(s)	49, 105, 381, 1074, 1239
BtuMI	TCGCGA	1 cut(s)	729
Cac8I	GCNNGC	5 cut(s)	388, 663, 894, 978, 1384
Cfr13I	GGNCC	6 cut(s)	347, 926, 1056, 1217, 1218, 1365
Cfr9I	CCCGGG	1 cut(s)	478
Csp6I	GTAC	4 cut(s)	197, 209, 514, 1506
CviAII	CATG	6 cut(s)	56, 160, 893, 1084, 1123, 1227
CviQI	GTAC	4 cut(s)	197, 209, 514, 1506
DdeI	CTNAG	6 cut(s)	250, 601, 672, 1160, 1242, 1262
DpnI	GATC	4 cut(s)	74, 164, 247, 1070
DpnII	GATC	4 cut(s)	72, 162, 245, 1068
DraIII	CACNNNGTG	1 cut(s)	1274
EaeI	YGGCCR	1 cut(s)	1125
Eam1104I	CTCTTC	1 cut(s)	1446
EarI	CTCTTC	1 cut(s)	1446
EciI	GGCGGA	1 cut(s)	839
Eco130I	CCWWGG	4 cut(s)	635, 1122, 1434, 1446
Eco147I	AGGCCT	1 cut(s)	462
Eco24I	GRGCYC	1 cut(s)	1221
Eco31I	GGTCTC	1 cut(s)	1424
Eco32I	GATATC	1 cut(s)	733
Eco47I	GGWCC	2 cut(s)	347, 926
Eco57I	CTGAAG	1 cut(s)	1150
Eco88I	CYCGRG	2 cut(s)	478, 626
EcoO109I	RGGNCCY	3 cut(s)	1056, 1217, 1365
EcoRI	GAATTC	1 cut(s)	585
EcoRII	CCWGG	2 cut(s)	1035, 1212
EcoRV	GATATC	1 cut(s)	733
EcoT14I	CCWWGG	4 cut(s)	635, 1122, 1434, 1446
EcoT38I	GRGCYC	1 cut(s)	1221
ErhI	CCWWGG	4 cut(s)	635, 1122, 1434, 1446
FaeI	CATG	6 cut(s)	59, 163, 896, 1087, 1126, 1230
FalI	AAGNNNNNCTT	2 cut(s)	702, 734
FaqI	GGGAC	1 cut(s)	616
FatI	CATG	6 cut(s)	55, 159, 892, 1083, 1122, 1226
FauI	CCCGC	1 cut(s)	850
FbaI	TGATCA	1 cut(s)	1068
Fnu4HI	GCNGC	3 cut(s)	255, 744, 867
FokI	GGATG	2 cut(s)	7, 570
FriOI	GRGCYC	1 cut(s)	1221
Fsp4HI	GCNGC	3 cut(s)	255, 744, 867
FspBI	CTAG	9 cut(s)	239, 302, 572, 662, 689, 798, 1097, 1278, 1488
GluI	GCNGC	3 cut(s)	255, 744, 867
GsaI	CCCAGC	2 cut(s)	951, 1050
GsuI	CTGGAG	1 cut(s)	405
HaeIII	GGCC	8 cut(s)	462, 835, 1057, 1127, 1156, 1212, 1219, 1367
HapII	CCGG	3 cut(s)	65, 479, 853
Hin1II	CATG	6 cut(s)	59, 163, 896, 1087, 1126, 1230
HindIII	AAGCTT	1 cut(s)	1162
HinfI	GANTC	6 cut(s)	501, 613, 629, 750, 876, 1258
HpaII	CCGG	3 cut(s)	65, 479, 853
HphI	GGTGA	3 cut(s)	658, 790, 815
Hpy166II	GTNNAC	3 cut(s)	381, 427, 1234
Hpy188I	TCNGA	3 cut(s)	271, 536, 557
Hpy188III	TCNNGA	4 cut(s)	456, 582, 728, 1306
Hpy8I	GTNNAC	3 cut(s)	381, 427, 1234
Hpy99I	CGWCG	2 cut(s)	194, 758
HpyAV	CCTTC	5 cut(s)	473, 600, 775, 800, 1378
HpyCH4III	ACNGT	4 cut(s)	985, 1065, 1318, 1510
HpyCH4IV	ACGT	2 cut(s)	516, 1031
HpyF10VI	GCNNNNNNNGC	8 cut(s)	104, 387, 658, 1080, 1274, 1319, 1388, 1397
HpyF3I	CTNAG	6 cut(s)	250, 601, 672, 1160, 1242, 1262
HpySE526I	ACGT	2 cut(s)	516, 1031
Hsp92II	CATG	6 cut(s)	59, 163, 896, 1087, 1126, 1230
KpnI	GGTACC	1 cut(s)	1509
Ksp22I	TGATCA	1 cut(s)	1068
Kzo9I	GATC	4 cut(s)	72, 162, 245, 1068
LmnI	GCTCC	3 cut(s)	155, 424, 480
Lsp1109I	GCAGC	3 cut(s)	266, 730, 878
LweI	GCATC	2 cut(s)	639, 1474
MaeI	CTAG	9 cut(s)	239, 302, 572, 662, 689, 798, 1097, 1278, 1488
MaeII	ACGT	2 cut(s)	516, 1031
MaeIII	GTNAC	2 cut(s)	684, 997
MalI	GATC	4 cut(s)	74, 164, 247, 1070
MboI	GATC	4 cut(s)	72, 162, 245, 1068
MboII	GAAGA	9 cut(s)	157, 188, 273, 719, 782, 1192, 1411, 1452, 1463
MfeI	CAATTG	3 cut(s)	594, 1206, 1356
MflI	RGATCY	1 cut(s)	245
MhlI	GDGCHC	5 cut(s)	238, 383, 1134, 1221, 1270
MlyI	GAGTC	1 cut(s)	638
MmeI	TCCRAC	2 cut(s)	853, 967
MroXI	GAANNNNTTC	1 cut(s)	589
MseI	TTAA	2 cut(s)	320, 506
MspA1I	CMGCKG	3 cut(s)	257, 845, 869
MspI	CCGG	3 cut(s)	65, 479, 853
MspR9I	CCNGG	5 cut(s)	65, 479, 480, 1037, 1214
MunI	CAATTG	3 cut(s)	594, 1206, 1356
Mva1269I	GAATGC	1 cut(s)	1315
MvaI	CCWGG	2 cut(s)	1037, 1214
MvnI	CGCG	1 cut(s)	729
MwoI	GCNNNNNNNGC	8 cut(s)	104, 387, 658, 1080, 1274, 1319, 1388, 1397
NciI	CCSGG	3 cut(s)	65, 479, 480
NcoI	CCATGG	1 cut(s)	1122
NdeII	GATC	4 cut(s)	72, 162, 245, 1068
NheI	GCTAGC	1 cut(s)	661
NlaIII	CATG	6 cut(s)	59, 163, 896, 1087, 1126, 1230
NlaIV	GGNNCC	7 cut(s)	157, 247, 1058, 1218, 1219, 1366, 1507
NmeAIII	GCCGAG	2 cut(s)	739, 1153
NmuCI	GTSAC	1 cut(s)	997
NruI	TCGCGA	1 cut(s)	729
NspI	RCATGY	1 cut(s)	896
PaeI	GCATGC	1 cut(s)	896
PceI	AGGCCT	1 cut(s)	462
PctI	GAATGC	1 cut(s)	1315
PdmI	GAANNNNTTC	1 cut(s)	589
PfeI	GAWTC	5 cut(s)	501, 613, 750, 876, 1258
PflMI	CCANNNNNTGG	1 cut(s)	1227
PfoI	TCCNGGA	1 cut(s)	1035
PkrI	GCNGC	3 cut(s)	256, 745, 868
PleI	GAGTC	1 cut(s)	637
PpsI	GAGTC	1 cut(s)	637
Ppu21I	YACGTR	1 cut(s)	517
Psp1406I	AACGTT	1 cut(s)	1031
Psp6I	CCWGG	2 cut(s)	1035, 1212
PspFI	CCCAGC	2 cut(s)	947, 1046
PspGI	CCWGG	2 cut(s)	1035, 1212
PspN4I	GGNNCC	7 cut(s)	157, 247, 1058, 1218, 1219, 1366, 1507
PspOMI	GGGCCC	1 cut(s)	1217
PspPI	GGNCC	6 cut(s)	347, 926, 1056, 1217, 1218, 1365
PstI	CTGCAG	1 cut(s)	436
PsuI	RGATCY	1 cut(s)	245
PvuII	CAGCTG	1 cut(s)	257
RruI	TCGCGA	1 cut(s)	729
RsaI	GTAC	4 cut(s)	198, 210, 515, 1507
RsaNI	GTAC	4 cut(s)	197, 209, 514, 1506
SaqAI	TTAA	2 cut(s)	320, 506
SatI	GCNGC	3 cut(s)	255, 744, 867
Sau3AI	GATC	4 cut(s)	72, 162, 245, 1068
Sau96I	GGNCC	6 cut(s)	347, 926, 1056, 1217, 1218, 1365
ScaI	AGTACT	1 cut(s)	210
SchI	GAGTC	1 cut(s)	638
ScrFI	CCNGG	5 cut(s)	65, 479, 480, 1037, 1214
SduI	GDGCHC	5 cut(s)	238, 383, 1134, 1221, 1270
SfaNI	GCATC	2 cut(s)	639, 1474
SfcI	CTRYAG	2 cut(s)	432, 1314
SinI	GGWCC	2 cut(s)	347, 926
SmaI	CCCGGG	1 cut(s)	480
SpeI	ACTAGT	1 cut(s)	688
SphI	GCATGC	1 cut(s)	896
SseBI	AGGCCT	1 cut(s)	462
SsiI	CCGC	3 cut(s)	843, 850, 869
SspI	AATATT	1 cut(s)	13
SspMI	CTAG	9 cut(s)	239, 302, 572, 662, 689, 798, 1097, 1278, 1488
StuI	AGGCCT	1 cut(s)	462
StyD4I	CCNGG	5 cut(s)	63, 477, 478, 1035, 1212
StyI	CCWWGG	4 cut(s)	635, 1122, 1434, 1446
TaaI	ACNGT	4 cut(s)	985, 1065, 1318, 1510
TaiI	ACGT	2 cut(s)	519, 1034
TaqI	TCGA	3 cut(s)	189, 204, 753
TaqII	GACCGA	1 cut(s)	269
TatI	WGTACW	2 cut(s)	196, 208
TfiI	GAWTC	5 cut(s)	501, 613, 750, 876, 1258
Tru1I	TTAA	2 cut(s)	320, 506
Tru9I	TTAA	2 cut(s)	320, 506
TscAI	CASTG	5 cut(s)	49, 105, 388, 1081, 1246
TseFI	GTSAC	1 cut(s)	997
TseI	GCWGC	3 cut(s)	254, 743, 866
Tsp45I	GTSAC	1 cut(s)	997
TspDTI	ATGAA	5 cut(s)	72, 157, 432, 850, 1321
TspMI	CCCGGG	1 cut(s)	478
TspRI	CASTG	5 cut(s)	49, 105, 388, 1081, 1246
Van91I	CCANNNNNTGG	1 cut(s)	1227
VneI	GTGCAC	1 cut(s)	379
VpaK11BI	GGWCC	2 cut(s)	347, 926
XapI	RAATTY	3 cut(s)	438, 585, 933
XceI	RCATGY	1 cut(s)	896
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1033
XmaI	CCCGGG	1 cut(s)	478
XmnI	GAANNNNTTC	1 cut(s)	589
XspI	CTAG	9 cut(s)	239, 302, 572, 662, 689, 798, 1097, 1278, 1488
ZrmI	AGTACT	1 cut(s)	210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.