Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010809

Physical Location & Seq

Genomic Coordinates Forward (+)

12866564 .. 12868455

Gene Structure 1892 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1155200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 684 bp

ATGGCAAGTGGTTTTGGGGAGTCAACAAACGTCCCGCCCCAAAGCGTCTCTTGCTCCGGTAACAATGCCAACGATGTGGGAGATTTCGAATGCAATATTTGCTTCGAACTAGCCCAAGACCCGATCATAACCCGTTGCGGCCACCTCTTTTGCTGGCCTTGCCTCTACAGGTGGCTCCACCATCACTCGAATTCACAAGAGTGCCCGTTTTGCAAGGCCCTCATAGAGGAAGAGAAGTTGGTTCCGCTTTATGGTAGGGGCAAGACGCAAACTGACCCAAGATCAAAGTCGTATCCAGGGATCAACATTCCCAATCGCCCGTCTGGGCAGAGGCCTCCAACTGCCCCTCCTCCGAACACCAATCAATTTGCCAATTATGGCTTTGGATTCATGGGGGGATTTGTACCAATGGCAACTCAACGGATTGGTAACTTCACCTTGGCGACTGCATTTGGTGGTCTGTTACCATCTTTGCTTAACGTTCAGTATCACGGGTTCCCGGATGCTACTGTATATGGAACAACTTCTGGTTTCCCTTTCGCACCATTCAATTCATTTCACGGAGGCCATGGTCACGGATTCGTTCAGCCGGAAAACCACCAATGGCAGCCGGCTGATGTTTGGAAGTATTTTTTTTTGTTTATCGGTGTGTTTGTGATCGTGGCGGTTATTTTTGGTTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006928 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016477 GO:0016567 GO:0016740 GO:0018996 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030054 GO:0030154 GO:0030163 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0040011 GO:0040039 GO:0042175 GO:0042221 GO:0042303 GO:0042692 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044449 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0048870 GO:0050896 GO:0051146 GO:0051179 GO:0051603 GO:0051674 GO:0051716 GO:0051788 GO:0055001 GO:0055002 GO:0055120 GO:0061061 GO:0061630 GO:0061659 GO:0070647 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0098796 GO:0098827 GO:0099080 GO:0099081 GO:0099512 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

228

Amino Acids

24.98

Weight (kDa)

6.48

Isoelectric Point (pI)

47.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	29 - 76	7.1e-09	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	30 - 71	8.8e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	30 - 72	1.5e-08	Ring finger domain
zf-RING_5	PF14634	30 - 72	3.1e-08	zinc-RING finger domain
zf-C3HC4	PF00097	31 - 71	3.9e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	31 - 69	8.3e-08	RING-type zinc-finger
RING_XB3-XBAT31	PF24921	31 - 75	2.1e-06	E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-C3HC4_4	PF15227	31 - 71	7.2e-06	zinc finger of C3HC4-type, RING

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23780 AT2G23780 AT2G23780
fragaria_vesca	FvH4_1g14110
malus_domestica	MD02G1155200.v1.1
prunus_persica	Prupe.7G148100_v2.0.a1 Prupe.7G148100_v2.0.a1
pyrus_communis	pycom02g12290 pycom15g23390
rosa_chinensis	RchiOBHm_Chr2g0102791
rosa_laevigata	RLG00000017179
rosa_roxburghii	Rroxscaffold_2G00140320
rosa_rugosa	Rorug02G0109400 Rorug02G0109500 Rorug02G0109500
rosa_samantha	Rh2AG157900 Rh2BG164400 Rh2CG164100 Rh2DG163200
rosa_wichuraiana	Rw2G012260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	4 cut(s)	35, 138, 245, 665
AclI	AACGTT	1 cut(s)	480
AclWI	GGATC	1 cut(s)	308
AcoI	YGGCCR	1 cut(s)	139
AcsI	RAATTY	1 cut(s)	190
AfaI	GTAC	1 cut(s)	405
AfiI	CCNNNNNNNGG	2 cut(s)	226, 251
AgsI	TTSAA	1 cut(s)	550
AjnI	CCWGG	1 cut(s)	295
AleI	CACNNNNGTG	1 cut(s)	199
Alw26I	GTCTC	1 cut(s)	52
AlwI	GGATC	1 cut(s)	308
AoxI	GGCC	5 cut(s)	139, 155, 216, 332, 565
ApeKI	GCWGC	1 cut(s)	607
ApoI	RAATTY	1 cut(s)	190
Asp700I	GAANNNNTTC	1 cut(s)	523
AspS9I	GGNCC	1 cut(s)	217
AsuC2I	CCSGG	1 cut(s)	500
AsuHPI	GGTGA	1 cut(s)	427
AsuII	TTCGAA	2 cut(s)	87, 105
BaeGI	GKGCMC	1 cut(s)	206
BbvI	GCAGC	1 cut(s)	619
BccI	CCATC	2 cut(s)	189, 475
BciT130I	CCWGG	1 cut(s)	297
BciVI	GTATCC	1 cut(s)	303
BcnI	CCSGG	1 cut(s)	500
BcoDI	GTCTC	1 cut(s)	52
BfaI	CTAG	1 cut(s)	110
BfmI	CTRYAG	1 cut(s)	166
BfuI	GTATCC	1 cut(s)	303
BisI	GCNGC	2 cut(s)	139, 608
BlsI	GCNGC	2 cut(s)	140, 609
Bme1390I	CCNGG	2 cut(s)	297, 500
BmgT120I	GGNCC	1 cut(s)	217
BmiI	GGNNCC	3 cut(s)	176, 243, 497
BmrFI	CCNGG	2 cut(s)	297, 500
BmsI	GCATC	1 cut(s)	493
Bpu14I	TTCGAA	2 cut(s)	87, 105
BpuMI	CCSGG	1 cut(s)	500
BsaJI	CCNNGG	3 cut(s)	296, 438, 568
BsaWI	WCCGGW	1 cut(s)	56
BsaXI	ACNNNNNCTCC	2 cut(s)	331, 361
Bsc4I	CCNNNNNNNGG	2 cut(s)	226, 251
Bse118I	RCCGGY	1 cut(s)	610
BseBI	CCWGG	1 cut(s)	297
BseDI	CCNNGG	3 cut(s)	296, 438, 568
BseGI	GGATG	1 cut(s)	508
BseLI	CCNNNNNNNGG	2 cut(s)	226, 251
BseRI	GAGGAG	1 cut(s)	339
BseSI	GKGCMC	1 cut(s)	206
BseXI	GCAGC	1 cut(s)	619
BshFI	GGCC	5 cut(s)	141, 157, 218, 334, 567
BsiSI	CCGG	4 cut(s)	57, 500, 590, 611
BslFI	GGGAC	1 cut(s)	17
BslI	CCNNNNNNNGG	2 cut(s)	226, 251
BsmAI	GTCTC	1 cut(s)	52
BsmBI	CGTCTC	1 cut(s)	52
BsmFI	GGGAC	1 cut(s)	17
BsmI	GAATGC	1 cut(s)	95
BsnI	GGCC	5 cut(s)	141, 157, 218, 334, 567
Bsp119I	TTCGAA	2 cut(s)	87, 105
Bsp1286I	GDGCHC	1 cut(s)	206
Bsp143I	GATC	4 cut(s)	123, 281, 300, 657
Bsp19I	CCATGG	1 cut(s)	568
BspACI	CCGC	4 cut(s)	35, 138, 245, 665
BspANI	GGCC	5 cut(s)	141, 157, 218, 334, 567
BspLI	GGNNCC	3 cut(s)	176, 243, 497
BspPI	GGATC	1 cut(s)	308
BspT104I	TTCGAA	2 cut(s)	87, 105
BsrFI	RCCGGY	1 cut(s)	610
BssAI	RCCGGY	1 cut(s)	610
BssECI	CCNNGG	3 cut(s)	296, 438, 568
BssMI	GATC	4 cut(s)	123, 281, 300, 657
BssT1I	CCWWGG	2 cut(s)	438, 568
Bst2UI	CCWGG	1 cut(s)	297
Bst4CI	ACNGT	1 cut(s)	511
Bst6I	CTCTTC	1 cut(s)	225
BstAPI	GCANNNNNTGC	1 cut(s)	99
BstBI	TTCGAA	2 cut(s)	87, 105
BstC8I	GCNNGC	2 cut(s)	155, 612
BstDSI	CCRYGG	1 cut(s)	568
BstENI	CCTNNNNNAGG	1 cut(s)	224
BstF5I	GGATG	1 cut(s)	508
BstKTI	GATC	4 cut(s)	126, 284, 303, 660
BstMAI	GTCTC	1 cut(s)	52
BstMBI	GATC	4 cut(s)	123, 281, 300, 657
BstMWI	GCNNNNNNNGC	4 cut(s)	51, 99, 159, 210
BstNI	CCWGG	1 cut(s)	297
BstSCI	CCNGG	2 cut(s)	295, 498
BstSFI	CTRYAG	1 cut(s)	166
BstSLI	GKGCMC	1 cut(s)	206
BstV1I	GCAGC	1 cut(s)	619
BstXI	CCANNNNNNTGG	1 cut(s)	76
BsuI	GTATCC	1 cut(s)	303
BsuRI	GGCC	5 cut(s)	141, 157, 218, 334, 567
BtgI	CCRYGG	1 cut(s)	568
BtsCI	GGATG	1 cut(s)	508
Cac8I	GCNNGC	2 cut(s)	155, 612
Cfr10I	RCCGGY	1 cut(s)	610
Cfr13I	GGNCC	1 cut(s)	217
CseI	GACGC	2 cut(s)	34, 274
Csp6I	GTAC	1 cut(s)	404
CspCI	CAANNNNNGTGG	2 cut(s)	131, 166
CviAII	CATG	2 cut(s)	391, 569
CviQI	GTAC	1 cut(s)	404
DpnI	GATC	4 cut(s)	125, 283, 302, 659
DpnII	GATC	4 cut(s)	123, 281, 300, 657
EaeI	YGGCCR	1 cut(s)	139
Eam1104I	CTCTTC	1 cut(s)	225
EarI	CTCTTC	1 cut(s)	225
Eco130I	CCWWGG	2 cut(s)	438, 568
Eco147I	AGGCCT	1 cut(s)	334
EcoNI	CCTNNNNNAGG	1 cut(s)	224
EcoO109I	RGGNCCY	1 cut(s)	217
EcoRI	GAATTC	1 cut(s)	190
EcoRII	CCWGG	1 cut(s)	295
EcoT14I	CCWWGG	2 cut(s)	438, 568
ErhI	CCWWGG	2 cut(s)	438, 568
Esp3I	CGTCTC	1 cut(s)	52
FaeI	CATG	2 cut(s)	394, 572
FaiI	YATR	8 cut(s)	128, 224, 252, 378, 392, 514, 516, 570
FalI	AAGNNNNNCTT	2 cut(s)	34, 66
FaqI	GGGAC	1 cut(s)	17
FatI	CATG	2 cut(s)	390, 568
FauI	CCCGC	1 cut(s)	42
Fnu4HI	GCNGC	2 cut(s)	139, 608
FokI	GGATG	1 cut(s)	515
Fsp4HI	GCNGC	2 cut(s)	139, 608
FspBI	CTAG	1 cut(s)	110
GluI	GCNGC	2 cut(s)	139, 608
HaeIII	GGCC	5 cut(s)	141, 157, 218, 334, 567
HapII	CCGG	4 cut(s)	57, 500, 590, 611
HgaI	GACGC	2 cut(s)	34, 274
Hin1II	CATG	2 cut(s)	394, 572
HincII	GTYRAC	1 cut(s)	24
HindII	GTYRAC	1 cut(s)	24
HinfI	GANTC	3 cut(s)	20, 387, 579
HpaII	CCGG	4 cut(s)	57, 500, 590, 611
HphI	GGTGA	1 cut(s)	427
Hpy166II	GTNNAC	1 cut(s)	24
Hpy188I	TCNGA	1 cut(s)	354
Hpy8I	GTNNAC	1 cut(s)	24
HpyCH4III	ACNGT	1 cut(s)	511
HpyCH4IV	ACGT	2 cut(s)	30, 480
HpyCH4V	TGCA	3 cut(s)	93, 213, 449
HpyF10VI	GCNNNNNNNGC	4 cut(s)	51, 99, 159, 210
HpySE526I	ACGT	2 cut(s)	30, 480
Hsp92II	CATG	2 cut(s)	394, 572
KroI	GCCGGC	1 cut(s)	610
KroNI	GCCGGC	1 cut(s)	612
Kzo9I	GATC	4 cut(s)	123, 281, 300, 657
LmnI	GCTCC	2 cut(s)	59, 180
Lsp1109I	GCAGC	1 cut(s)	619
LweI	GCATC	1 cut(s)	493
MaeI	CTAG	1 cut(s)	110
MaeII	ACGT	2 cut(s)	30, 480
MaeIII	GTNAC	4 cut(s)	59, 428, 462, 572
MalI	GATC	4 cut(s)	125, 283, 302, 659
MboI	GATC	4 cut(s)	123, 281, 300, 657
MboII	GAAGA	1 cut(s)	242
MhlI	GDGCHC	1 cut(s)	206
MluCI	AATT	4 cut(s)	190, 365, 373, 550
MlyI	GAGTC	1 cut(s)	29
MmeI	TCCRAC	1 cut(s)	362
MnlI	CCTC	9 cut(s)	155, 173, 220, 230, 324, 345, 357, 360, 557
MroNI	GCCGGC	1 cut(s)	610
MroXI	GAANNNNTTC	1 cut(s)	523
MseI	TTAA	1 cut(s)	477
MslI	CAYNNNNRTG	1 cut(s)	199
MspI	CCGG	4 cut(s)	57, 500, 590, 611
MspR9I	CCNGG	2 cut(s)	297, 500
Mva1269I	GAATGC	1 cut(s)	95
MvaI	CCWGG	1 cut(s)	297
MwoI	GCNNNNNNNGC	4 cut(s)	51, 99, 159, 210
NaeI	GCCGGC	1 cut(s)	612
NciI	CCSGG	1 cut(s)	500
NcoI	CCATGG	1 cut(s)	568
NdeII	GATC	4 cut(s)	123, 281, 300, 657
NgoMIV	GCCGGC	1 cut(s)	610
NlaIII	CATG	2 cut(s)	394, 572
NlaIV	GGNNCC	3 cut(s)	176, 243, 497
NmuCI	GTSAC	1 cut(s)	572
NspV	TTCGAA	2 cut(s)	87, 105
OliI	CACNNNNGTG	1 cut(s)	199
PceI	AGGCCT	1 cut(s)	334
PctI	GAATGC	1 cut(s)	95
PdiI	GCCGGC	1 cut(s)	612
PdmI	GAANNNNTTC	1 cut(s)	523
PfeI	GAWTC	2 cut(s)	387, 579
PfoI	TCCNGGA	1 cut(s)	498
PkrI	GCNGC	2 cut(s)	140, 609
PleI	GAGTC	1 cut(s)	28
PpsI	GAGTC	1 cut(s)	28
Psp1406I	AACGTT	1 cut(s)	480
Psp6I	CCWGG	1 cut(s)	295
PspGI	CCWGG	1 cut(s)	295
PspN4I	GGNNCC	3 cut(s)	176, 243, 497
PspPI	GGNCC	1 cut(s)	217
PsrI	GAACNNNNNNTAC	2 cut(s)	479, 511
RsaI	GTAC	1 cut(s)	405
RsaNI	GTAC	1 cut(s)	404
RseI	CAYNNNNRTG	1 cut(s)	199
SaqAI	TTAA	1 cut(s)	477
SatI	GCNGC	2 cut(s)	139, 608
Sau3AI	GATC	4 cut(s)	123, 281, 300, 657
Sau96I	GGNCC	1 cut(s)	217
SchI	GAGTC	1 cut(s)	29
ScrFI	CCNGG	2 cut(s)	297, 500
SduI	GDGCHC	1 cut(s)	206
SetI	ASST	5 cut(s)	33, 147, 173, 440, 483
SfaNI	GCATC	1 cut(s)	493
SfcI	CTRYAG	1 cut(s)	166
SfuI	TTCGAA	2 cut(s)	87, 105
SmiMI	CAYNNNNRTG	1 cut(s)	199
Sse9I	AATT	4 cut(s)	190, 365, 373, 550
SseBI	AGGCCT	1 cut(s)	334
SsiI	CCGC	4 cut(s)	35, 138, 245, 665
SspI	AATATT	1 cut(s)	97
SspMI	CTAG	1 cut(s)	110
StuI	AGGCCT	1 cut(s)	334
StyD4I	CCNGG	2 cut(s)	295, 498
StyI	CCWWGG	2 cut(s)	438, 568
TaaI	ACNGT	1 cut(s)	511
TaiI	ACGT	2 cut(s)	33, 483
TaqI	TCGA	3 cut(s)	87, 105, 188
TasI	AATT	4 cut(s)	190, 365, 373, 550
TauI	GCSGC	1 cut(s)	141
TfiI	GAWTC	2 cut(s)	387, 579
Tru1I	TTAA	1 cut(s)	477
Tru9I	TTAA	1 cut(s)	477
TseFI	GTSAC	1 cut(s)	572
TseI	GCWGC	1 cut(s)	607
Tsp45I	GTSAC	1 cut(s)	572
TspDTI	ATGAA	2 cut(s)	379, 543
TspGWI	ACGGA	3 cut(s)	436, 576, 591
XagI	CCTNNNNNAGG	1 cut(s)	224
XapI	RAATTY	1 cut(s)	190
XmnI	GAANNNNTTC	1 cut(s)	523
XspI	CTAG	1 cut(s)	110

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.